CmaCh06G006710.1 (mRNA) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh06G006710.1
Type	mRNA
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	transmembrane emp24 domain-containing protein p24beta2-like
Location	Cma_Chr06: 3335327 .. 3336941 (+)
Sequence length	796
RNA-Seq Expression	CmaCh06G006710.1
Synteny	CmaCh06G006710.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCATTCCTTGAATTCATTCGATTCTGTTCATTTGTGGAGTTCAAGAACAAAATGGAGATGGGAAGTGGGAAGTCAAAATACAGGTTTTTTATTTTCATGGCGGCTTTGTTCATATCATCACACCCAGTTCTGGGGATTAGATTTGTGATCGACAAGGAAGAATGCTTCTCTCACAATGTTGAATTCATCAGAGATACCATTTATCTCTCTTTTGTCGTCGTTAGCGTTAATACCTGGCGGCATCATGCTCACAATGGCGTTGATCTTTTGGTGGCCACTTCTTTCTCTCTCTCTCTCTCTCTCTTAGATTCATCCAGATTGTTCATATCATTTGCATGTTGTTTATGAGATGTTCGGCTCGTGAATTATCAGGGTTAAGACTTTGAATCTGATTTTACATACGTGTTGCTGTTACCTACCTTACAGAGGTCAAGGAACTGAGCCTTTTGTTTCGTATGTATGGATGAATGAGATTCACATTAGGGTTTAAATATATGTTGTCTATGAGATGTTCGGCTCGTGCATTATCAGGGTTAAGACTTTGAATCTGATTTTACATGCGTGTTGCTGTTACCTACCTTACAGAGGTCAAGGAACTGAGCCTTTTGTTTCGTATGTATGGATGAATGAGATTCACATTAGGGTTTGAATATATGTTGTTATAAGTTTTTTCAAGGTGTTTGTATTCGGTTTATGGATTAAGTTTTTGTACGACACAACAAGTTGTATACAAGTTTTTTTCTTATTTGAAGCTAGCTAGTGACTGTCAATTCCATGTGGATAGTAACATTTACATATCATATATAATACAAATCAATTTCATAAATCTTGTTTTAGGCTTGTTATGATTAAATGTGATTTGCAAAGTACAGGTGAAAGGGCCTTTAGGGGAGAGAATTGAAGAAATTAGAGGGAAAACAAGTGAGAAATTTGAGTTTATTTCTCTTAGAAAAGGGCGATATCATTTCTGCTTCATCAACAACTCACCCCACCAAGAGACCATAGAATTTGATATCCATATCAATCATTACTATGAGTTCTATGATGATGATGAACAACATGTAAAAGATGGTATTGATTCCTTTGATGAACCCTAAAGTTTTTACTTTGAAATTCATACTTCCCTTTACTTTTCTGCTCATCTCATCTGGTTTGAAATGAAATTTACAGAACATTTGAGCCCTTTATTGGCTCAAATAAAGAAGTTGGGAGAAACCTTGGGCGTTATTGAGTTTGAACAGCAATGGTTAGAGGCACAGACAGAGCGACAAGCCAAAGGTATTGTTCATTTATTAACCCATTTCATATACAATCTATTCCTTTGTATTAATTCAAATATCAGATCTAAACAATAACTTCTAGCATTTAGTAAGTAAGTGAAGTACCTTTTTTGCAGTGAATGAAGCAATGAGCACAATGGCGATTCTGAAGGCTGTTCTCGAATCAGCAGCACTCATCGGTGCAAGCAGTCTTCAAGTTTTCCTTCTGCGTCGCCTGTTCGAGAAAAAAGTTGGAAGCCTGTATTCGAGCACCTACAGAATATAATCTCATATCCGAACTCCAGCAGACATACAACAACAAGCATGGCAGAACAAGTGCTGGAAAGGTTGAGGAG

mRNA sequence

CCATTCCTTGAATTCATTCGATTCTGTTCATTTGTGGAGTTCAAGAACAAAATGGAGATGGGAAGTGGGAAGTCAAAATACAGGTTTTTTATTTTCATGGCGGCTTTGTTCATATCATCACACCCAGTTCTGGGGATTAGATTTGTGATCGACAAGGAAGAATGCTTCTCTCACAATGTTGAATTCATCAGAGATACCATTTATCTCTCTTTTGTCGTCGTTAGCGTTAATACCTGGCGGCATCATGCTCACAATGGCGTTGATCTTTTGGTGAAAGGGCCTTTAGGGGAGAGAATTGAAGAAATTAGAGGGAAAACAAGTGAGAAATTTGAGTTTATTTCTCTTAGAAAAGGGCGATATCATTTCTGCTTCATCAACAACTCACCCCACCAAGAGACCATAGAATTTGATATCCATATCAATCATTACTATGAGTTCTATGATGATGATGAACAACATGTAAAAGATGAACATTTGAGCCCTTTATTGGCTCAAATAAAGAAGTTGGGAGAAACCTTGGGCGTTATTGAGTTTGAACAGCAATGGTTAGAGGCACAGACAGAGCGACAAGCCAAAGTGAATGAAGCAATGAGCACAATGGCGATTCTGAAGGCTGTTCTCGAATCAGCAGCACTCATCGGTGCAAGCAGTCTTCAAGTTTTCCTTCTGCGTCGCCTGTTCGAGAAAAAAGTTGGAAGCCTGTATTCGAGCACCTACAGAATATAATCTCATATCCGAACTCCAGCAGACATACAACAACAAGCATGGCAGAACAAGTGCTGGAAAGGTTGAGGAG

Coding sequence (CDS)

ATGGAGATGGGAAGTGGGAAGTCAAAATACAGGTTTTTTATTTTCATGGCGGCTTTGTTCATATCATCACACCCAGTTCTGGGGATTAGATTTGTGATCGACAAGGAAGAATGCTTCTCTCACAATGTTGAATTCATCAGAGATACCATTTATCTCTCTTTTGTCGTCGTTAGCGTTAATACCTGGCGGCATCATGCTCACAATGGCGTTGATCTTTTGGTGAAAGGGCCTTTAGGGGAGAGAATTGAAGAAATTAGAGGGAAAACAAGTGAGAAATTTGAGTTTATTTCTCTTAGAAAAGGGCGATATCATTTCTGCTTCATCAACAACTCACCCCACCAAGAGACCATAGAATTTGATATCCATATCAATCATTACTATGAGTTCTATGATGATGATGAACAACATGTAAAAGATGAACATTTGAGCCCTTTATTGGCTCAAATAAAGAAGTTGGGAGAAACCTTGGGCGTTATTGAGTTTGAACAGCAATGGTTAGAGGCACAGACAGAGCGACAAGCCAAAGTGAATGAAGCAATGAGCACAATGGCGATTCTGAAGGCTGTTCTCGAATCAGCAGCACTCATCGGTGCAAGCAGTCTTCAAGTTTTCCTTCTGCGTCGCCTGTTCGAGAAAAAAGTTGGAAGCCTGTATTCGAGCACCTACAGAATATAA

Protein sequence

MEMGSGKSKYRFFIFMAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVNTWRHHAHNGVDLLVKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQHVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAALIGASSLQVFLLRRLFEKKVGSLYSSTYRI

Homology

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BLAST of CmaCh06G006710.1 vs. ExPASy Swiss-Prot
Match: Q9S7M9 (Transmembrane emp24 domain-containing protein p24beta2 OS=Arabidopsis thaliana OX=3702 GN=At3g07680 PE=1 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 1.7e-53
Identity = 110/202 (54.46%), Postives = 143/202 (70.79%), Query Frame = 0


Query: 14  IFMAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVNTWRHHAHNGVDLL 73
           I +  L  S    LGIRFVID+EECFSH  E+  DT+++SFVV+  ++  H   +GVDL+
Sbjct: 7   IVLLGLLWSFQATLGIRFVIDREECFSHKAEYEGDTLHVSFVVIKSDSQWHFNEDGVDLV 66

Query: 74  VKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDD 133
           + GP GE+I + R + S K +F+  +KG Y FCF N SP+ ETI+FD+ + H + +YD  
Sbjct: 67  IHGPTGEQIHDFREQISAKHDFVVQKKGVYRFCFTNKSPYHETIDFDVQLGH-FAYYD-- 126

Query: 134 EQHVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESA 193
            QH KDEH +PL+ QI KL E L  I+FEQ WLEAQT+RQA VNE MS  A+ KA+ ES 
Sbjct: 127 -QHAKDEHFTPLMEQISKLEEALYNIQFEQHWLEAQTDRQAIVNENMSKRAVHKALFESF 186

Query: 194 ALIGASSLQVFLLRRLFEKKVG 216
           ALIGAS LQV+LLRRLFE+K+G
Sbjct: 187 ALIGASFLQVYLLRRLFERKLG 204

[Close]

BLAST of CmaCh06G006710.1 vs. ExPASy Swiss-Prot
Match: Q9LIL4 (Transmembrane emp24 domain-containing protein p24beta3 OS=Arabidopsis thaliana OX=3702 GN=At3g22845 PE=2 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 4.1e-23
Identity = 67/209 (32.06%), Postives = 108/209 (51.67%), Query Frame = 0


Query: 7   KSKYRFFIFMAALFISS-HPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVNTWRHH 66
           ++K   F+ +  + ++S + +  +   ++ EEC    V +  DT+  +FVVV  + +   
Sbjct: 5   QAKIHVFVLIGLILLNSINQISSLSVTVNDEECVQEYVLYEGDTVSGNFVVVDHDIFWGS 64

Query: 67  AHNGVDLLVKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINH 126
            H G+D  V  P G  ++ ++G + +KFEF + + G Y FCF N     ET+ F IH+ H
Sbjct: 65  DHPGLDFTVTSPAGNIVQTLKGTSGDKFEFKAPKSGMYKFCFHNPYSTPETVSFYIHVGH 124

Query: 127 YYEFYDDDEQHVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAI 186
                 ++    KDEHL P+  +I +L E L  +  EQ++L+A+  R    NE+     I
Sbjct: 125 ----IPNEHDLAKDEHLDPVNVKIAELREALESVVAEQKYLKARDTRHRHTNESTRKRVI 184

Query: 187 LKAVLESAALIGASSLQVFLLRRLFEKKV 215
              V E   L  AS LQV  +R+LF K V
Sbjct: 185 FYTVGEYIFLAAASGLQVLYIRKLFSKSV 209

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BLAST of CmaCh06G006710.1 vs. ExPASy TrEMBL
Match: A0A6J1KYW1 (transmembrane emp24 domain-containing protein p24beta2-like OS=Cucurbita maxima OX=3661 GN=LOC111498823 PE=3 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 6.5e-112
Identity = 209/209 (100.00%), Postives = 209/209 (100.00%), Query Frame = 0


Query: 16  MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVNTWRHHAHNGVDLLVK 75
           MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVNTWRHHAHNGVDLLVK
Sbjct: 1   MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVNTWRHHAHNGVDLLVK 60

Query: 76  GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ 135
           GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ
Sbjct: 61  GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ 120

Query: 136 HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAAL 195
           HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAAL
Sbjct: 121 HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAAL 180

Query: 196 IGASSLQVFLLRRLFEKKVGSLYSSTYRI 225
           IGASSLQVFLLRRLFEKKVGSLYSSTYRI
Sbjct: 181 IGASSLQVFLLRRLFEKKVGSLYSSTYRI 209

[Close]

BLAST of CmaCh06G006710.1 vs. ExPASy TrEMBL
Match: A0A6J1GJG3 (transmembrane emp24 domain-containing protein p24beta2-like OS=Cucurbita moschata OX=3662 GN=LOC111454883 PE=3 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 2.5e-111
Identity = 208/209 (99.52%), Postives = 209/209 (100.00%), Query Frame = 0


Query: 16  MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVNTWRHHAHNGVDLLVK 75
           MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSV+TWRHHAHNGVDLLVK
Sbjct: 1   MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVHTWRHHAHNGVDLLVK 60

Query: 76  GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ 135
           GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ
Sbjct: 61  GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ 120

Query: 136 HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAAL 195
           HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAAL
Sbjct: 121 HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAAL 180

Query: 196 IGASSLQVFLLRRLFEKKVGSLYSSTYRI 225
           IGASSLQVFLLRRLFEKKVGSLYSSTYRI
Sbjct: 181 IGASSLQVFLLRRLFEKKVGSLYSSTYRI 209

[Close]

BLAST of CmaCh06G006710.1 vs. ExPASy TrEMBL
Match: A0A6J1CS53 (transmembrane emp24 domain-containing protein p24beta2-like OS=Momordica charantia OX=3673 GN=LOC111013807 PE=3 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 1.3e-72
Identity = 150/224 (66.96%), Postives = 174/224 (77.68%), Query Frame = 0


Query: 1   MEMGSGKSKYRFFIFMAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVN 60
           M +G  K   R  +    + +S+  V G RFVIDKEECFSHNVEF RDTI+LSFVVVSV+
Sbjct: 1   MRIGKSKGWLRIAMVGLLVSVSAEAVFGFRFVIDKEECFSHNVEFTRDTIHLSFVVVSVH 60

Query: 61  TWRHHAHNGVDLLVKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFD 120
               HAHNGVDL VKGP GE IEE +GKTSEKF+F++  KG Y FCF N SPH ETI+FD
Sbjct: 61  ASWRHAHNGVDLSVKGPKGEGIEEHKGKTSEKFKFVAHFKGPYRFCFTNKSPHHETIDFD 120

Query: 121 IHINHYYEFYDDDEQHVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAM 180
           IHI  Y+E YD D QHV++EHLSPL+ QI+KL E L ++EFEQQWLEAQTERQ KVNEAM
Sbjct: 121 IHITRYFEPYDHD-QHVQNEHLSPLMDQIEKLREALDIVEFEQQWLEAQTERQVKVNEAM 180

Query: 181 STMAILKAVLESAALIGASSLQVFLLRRLFEKKVGSLYSSTYRI 225
           S  AILKA+LESAALIGAS LQV LL RLFEK+VG L+S++Y I
Sbjct: 181 SRWAILKALLESAALIGASGLQVCLLCRLFEKRVGVLHSNSYTI 223

[Close]

BLAST of CmaCh06G006710.1 vs. ExPASy TrEMBL
Match: A0A6J5XME6 (GOLD domain-containing protein OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCUS34477 PE=3 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 7.5e-60
Identity = 121/202 (59.90%), Postives = 154/202 (76.24%), Query Frame = 0


Query: 14  IFMAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVNTWRHHAHNGVDLL 73
           + +    +S   V GIRFVID+EECFSHNV+F  DT+++SFVV+ V+   H+  +GVDL+
Sbjct: 11  VLLLTFLVSLQAVFGIRFVIDREECFSHNVQFEGDTVHISFVVIKVDASWHYTQDGVDLV 70

Query: 74  VKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDD 133
           VKGP GE+I + R KTSEKF+F++  KG + FCF N SP+ ETI+FDIH++H + +YD  
Sbjct: 71  VKGPSGEQIHDFRDKTSEKFDFVTRHKGVHQFCFTNKSPYHETIDFDIHVSH-FTYYD-- 130

Query: 134 EQHVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESA 193
            QH KDEH +PLL Q+ KL E L  I+FEQ WLEAQTERQA VNEAMS  AI KA  ESA
Sbjct: 131 -QHAKDEHFNPLLEQVSKLEEALYNIQFEQHWLEAQTERQAIVNEAMSRRAIHKAFFESA 190

Query: 194 ALIGASSLQVFLLRRLFEKKVG 216
           AL+GAS+LQV+LLRRLFE+K+G
Sbjct: 191 ALVGASALQVYLLRRLFERKLG 208

[Close]

BLAST of CmaCh06G006710.1 vs. ExPASy TrEMBL
Match: M5WCT6 (GOLD domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_6G024700 PE=3 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 1.7e-59
Identity = 120/202 (59.41%), Postives = 154/202 (76.24%), Query Frame = 0


Query: 14  IFMAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVNTWRHHAHNGVDLL 73
           + +    +S   V GIRFVID+EECFSHNV++  DT+++SFVV+ V+   H+  +GVDL+
Sbjct: 11  VLLLTFLVSLQAVFGIRFVIDREECFSHNVQYEGDTVHISFVVIKVDASWHYTQDGVDLV 70

Query: 74  VKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDD 133
           VKGP GE+I + R KTSEKF+F++  KG + FCF N SP+ ETI+FDIH++H + +YD  
Sbjct: 71  VKGPSGEQIHDFRDKTSEKFDFVTRHKGVHQFCFTNKSPYHETIDFDIHVSH-FTYYD-- 130

Query: 134 EQHVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESA 193
            QH KDEH +PLL Q+ KL E L  I+FEQ WLEAQTERQA VNEAMS  AI KA  ESA
Sbjct: 131 -QHAKDEHFNPLLEQVSKLEEALYNIQFEQHWLEAQTERQAIVNEAMSRRAIHKAFFESA 190

Query: 194 ALIGASSLQVFLLRRLFEKKVG 216
           AL+GAS+LQV+LLRRLFE+K+G
Sbjct: 191 ALVGASALQVYLLRRLFERKLG 208

[Close]

BLAST of CmaCh06G006710.1 vs. NCBI nr
Match: KAG6596756.1 (Transmembrane emp24 domain-containing protein p24beta2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028293.1 Transmembrane emp24 domain-containing protein p24beta2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 441.0 bits (1133), Expect = 6.0e-120
Identity = 223/224 (99.55%), Postives = 224/224 (100.00%), Query Frame = 0


Query: 1   MEMGSGKSKYRFFIFMAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVN 60
           MEMGSGKSKYRFFIFMAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSV+
Sbjct: 1   MEMGSGKSKYRFFIFMAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVH 60

Query: 61  TWRHHAHNGVDLLVKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFD 120
           TWRHHAHNGVDLLVKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFD
Sbjct: 61  TWRHHAHNGVDLLVKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFD 120

Query: 121 IHINHYYEFYDDDEQHVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAM 180
           IHINHYYEFYDDDEQHVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAM
Sbjct: 121 IHINHYYEFYDDDEQHVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAM 180

Query: 181 STMAILKAVLESAALIGASSLQVFLLRRLFEKKVGSLYSSTYRI 225
           STMAILKAVLESAALIGASSLQVFLLRRLFEKKVGSLYSSTYRI
Sbjct: 181 STMAILKAVLESAALIGASSLQVFLLRRLFEKKVGSLYSSTYRI 224

[Close]

BLAST of CmaCh06G006710.1 vs. NCBI nr
Match: XP_023005965.1 (transmembrane emp24 domain-containing protein p24beta2-like [Cucurbita maxima])

HSP 1 Score: 413.3 bits (1061), Expect = 1.3e-111
Identity = 209/209 (100.00%), Postives = 209/209 (100.00%), Query Frame = 0


Query: 16  MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVNTWRHHAHNGVDLLVK 75
           MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVNTWRHHAHNGVDLLVK
Sbjct: 1   MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVNTWRHHAHNGVDLLVK 60

Query: 76  GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ 135
           GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ
Sbjct: 61  GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ 120

Query: 136 HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAAL 195
           HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAAL
Sbjct: 121 HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAAL 180

Query: 196 IGASSLQVFLLRRLFEKKVGSLYSSTYRI 225
           IGASSLQVFLLRRLFEKKVGSLYSSTYRI
Sbjct: 181 IGASSLQVFLLRRLFEKKVGSLYSSTYRI 209

[Close]

BLAST of CmaCh06G006710.1 vs. NCBI nr
Match: XP_022952151.1 (transmembrane emp24 domain-containing protein p24beta2-like [Cucurbita moschata])

HSP 1 Score: 411.4 bits (1056), Expect = 5.1e-111
Identity = 208/209 (99.52%), Postives = 209/209 (100.00%), Query Frame = 0


Query: 16  MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVNTWRHHAHNGVDLLVK 75
           MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSV+TWRHHAHNGVDLLVK
Sbjct: 1   MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVHTWRHHAHNGVDLLVK 60

Query: 76  GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ 135
           GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ
Sbjct: 61  GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ 120

Query: 136 HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAAL 195
           HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAAL
Sbjct: 121 HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAAL 180

Query: 196 IGASSLQVFLLRRLFEKKVGSLYSSTYRI 225
           IGASSLQVFLLRRLFEKKVGSLYSSTYRI
Sbjct: 181 IGASSLQVFLLRRLFEKKVGSLYSSTYRI 209

[Close]

BLAST of CmaCh06G006710.1 vs. NCBI nr
Match: XP_023539502.1 (transmembrane emp24 domain-containing protein p24beta2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 328.6 bits (841), Expect = 4.3e-86
Identity = 159/160 (99.38%), Postives = 160/160 (100.00%), Query Frame = 0


Query: 16  MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVNTWRHHAHNGVDLLVK 75
           MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSV+TWRHHAHNGVDLLVK
Sbjct: 1   MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVHTWRHHAHNGVDLLVK 60

Query: 76  GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ 135
           GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ
Sbjct: 61  GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ 120

Query: 136 HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAK 176
           HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAK
Sbjct: 121 HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAK 160

[Close]

BLAST of CmaCh06G006710.1 vs. NCBI nr
Match: XP_022144018.1 (transmembrane emp24 domain-containing protein p24beta2-like [Momordica charantia])

HSP 1 Score: 282.7 bits (722), Expect = 2.7e-72
Identity = 150/224 (66.96%), Postives = 174/224 (77.68%), Query Frame = 0


Query: 1   MEMGSGKSKYRFFIFMAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVN 60
           M +G  K   R  +    + +S+  V G RFVIDKEECFSHNVEF RDTI+LSFVVVSV+
Sbjct: 1   MRIGKSKGWLRIAMVGLLVSVSAEAVFGFRFVIDKEECFSHNVEFTRDTIHLSFVVVSVH 60

Query: 61  TWRHHAHNGVDLLVKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFD 120
               HAHNGVDL VKGP GE IEE +GKTSEKF+F++  KG Y FCF N SPH ETI+FD
Sbjct: 61  ASWRHAHNGVDLSVKGPKGEGIEEHKGKTSEKFKFVAHFKGPYRFCFTNKSPHHETIDFD 120

Query: 121 IHINHYYEFYDDDEQHVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAM 180
           IHI  Y+E YD D QHV++EHLSPL+ QI+KL E L ++EFEQQWLEAQTERQ KVNEAM
Sbjct: 121 IHITRYFEPYDHD-QHVQNEHLSPLMDQIEKLREALDIVEFEQQWLEAQTERQVKVNEAM 180

Query: 181 STMAILKAVLESAALIGASSLQVFLLRRLFEKKVGSLYSSTYRI 225
           S  AILKA+LESAALIGAS LQV LL RLFEK+VG L+S++Y I
Sbjct: 181 SRWAILKALLESAALIGASGLQVCLLCRLFEKRVGVLHSNSYTI 223

[Close]

BLAST of CmaCh06G006710.1 vs. TAIR 10
Match: AT3G07680.1 (emp24/gp25L/p24 family/GOLD family protein )

HSP 1 Score: 210.7 bits (535), Expect = 1.2e-54
Identity = 110/202 (54.46%), Postives = 143/202 (70.79%), Query Frame = 0


Query: 14  IFMAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVNTWRHHAHNGVDLL 73
           I +  L  S    LGIRFVID+EECFSH  E+  DT+++SFVV+  ++  H   +GVDL+
Sbjct: 7   IVLLGLLWSFQATLGIRFVIDREECFSHKAEYEGDTLHVSFVVIKSDSQWHFNEDGVDLV 66

Query: 74  VKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDD 133
           + GP GE+I + R + S K +F+  +KG Y FCF N SP+ ETI+FD+ + H + +YD  
Sbjct: 67  IHGPTGEQIHDFREQISAKHDFVVQKKGVYRFCFTNKSPYHETIDFDVQLGH-FAYYD-- 126

Query: 134 EQHVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESA 193
            QH KDEH +PL+ QI KL E L  I+FEQ WLEAQT+RQA VNE MS  A+ KA+ ES 
Sbjct: 127 -QHAKDEHFTPLMEQISKLEEALYNIQFEQHWLEAQTDRQAIVNENMSKRAVHKALFESF 186

Query: 194 ALIGASSLQVFLLRRLFEKKVG 216
           ALIGAS LQV+LLRRLFE+K+G
Sbjct: 187 ALIGASFLQVYLLRRLFERKLG 204

[Close]

BLAST of CmaCh06G006710.1 vs. TAIR 10
Match: AT3G22845.1 (emp24/gp25L/p24 family/GOLD family protein )

HSP 1 Score: 109.8 bits (273), Expect = 2.9e-24
Identity = 67/209 (32.06%), Postives = 108/209 (51.67%), Query Frame = 0


Query: 7   KSKYRFFIFMAALFISS-HPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVNTWRHH 66
           ++K   F+ +  + ++S + +  +   ++ EEC    V +  DT+  +FVVV  + +   
Sbjct: 5   QAKIHVFVLIGLILLNSINQISSLSVTVNDEECVQEYVLYEGDTVSGNFVVVDHDIFWGS 64

Query: 67  AHNGVDLLVKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINH 126
            H G+D  V  P G  ++ ++G + +KFEF + + G Y FCF N     ET+ F IH+ H
Sbjct: 65  DHPGLDFTVTSPAGNIVQTLKGTSGDKFEFKAPKSGMYKFCFHNPYSTPETVSFYIHVGH 124

Query: 127 YYEFYDDDEQHVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAI 186
                 ++    KDEHL P+  +I +L E L  +  EQ++L+A+  R    NE+     I
Sbjct: 125 ----IPNEHDLAKDEHLDPVNVKIAELREALESVVAEQKYLKARDTRHRHTNESTRKRVI 184

Query: 187 LKAVLESAALIGASSLQVFLLRRLFEKKV 215
              V E   L  AS LQV  +R+LF K V
Sbjct: 185 FYTVGEYIFLAAASGLQVLYIRKLFSKSV 209

The following BLAST results are available for this feature:

BLAST of CmaCh06G006710.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9S7M9	1.7e-53	54.46	Transmembrane emp24 domain-containing protein p24beta2 OS=Arabidopsis thaliana O...	[more]
Q9LIL4	4.1e-23	32.06	Transmembrane emp24 domain-containing protein p24beta3 OS=Arabidopsis thaliana O...	[more]

BLAST of CmaCh06G006710.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KYW1	6.5e-112	100.00	transmembrane emp24 domain-containing protein p24beta2-like OS=Cucurbita maxima ...	[more]
A0A6J1GJG3	2.5e-111	99.52	transmembrane emp24 domain-containing protein p24beta2-like OS=Cucurbita moschat...	[more]
A0A6J1CS53	1.3e-72	66.96	transmembrane emp24 domain-containing protein p24beta2-like OS=Momordica charant...	[more]
A0A6J5XME6	7.5e-60	59.90	GOLD domain-containing protein OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCUS34...	[more]
M5WCT6	1.7e-59	59.41	GOLD domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_6G024700 PE=3 ...	[more]

BLAST of CmaCh06G006710.1 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6596756.1	6.0e-120	99.55	Transmembrane emp24 domain-containing protein p24beta2, partial [Cucurbita argyr...	[more]
XP_023005965.1	1.3e-111	100.00	transmembrane emp24 domain-containing protein p24beta2-like [Cucurbita maxima]	[more]
XP_022952151.1	5.1e-111	99.52	transmembrane emp24 domain-containing protein p24beta2-like [Cucurbita moschata]	[more]
XP_023539502.1	4.3e-86	99.38	transmembrane emp24 domain-containing protein p24beta2-like [Cucurbita pepo subs...	[more]
XP_022144018.1	2.7e-72	66.96	transmembrane emp24 domain-containing protein p24beta2-like [Momordica charantia...	[more]

BLAST of CmaCh06G006710.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G07680.1	1.2e-54	54.46	emp24/gp25L/p24 family/GOLD family protein	[more]
AT3G22845.1	2.9e-24	32.06	emp24/gp25L/p24 family/GOLD family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009038	GOLD domain	SMART	SM01190	EMP24_GP25L_2	coord: 28..211 e-value: 7.2E-31 score: 118.6
IPR009038	GOLD domain	PFAM	PF01105	EMP24_GP25L	coord: 35..211 e-value: 4.3E-27 score: 95.2
IPR009038	GOLD domain	PROSITE	PS50866	GOLD	coord: 36..123 score: 11.675441
None	No IPR available	PANTHER	PTHR22811:SF167	TMP21-RELATED PROTEIN-RELATED	coord: 25..215
IPR015720	Transmembrane emp24 domain-containing protein	PANTHER	PTHR22811	TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN	coord: 25..215

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CmaCh06G006710	CmaCh06G006710	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh06G006710.1:exon:1314	CmaCh06G006710.1:exon:1314	exon
CmaCh06G006710.1:exon:1315	CmaCh06G006710.1:exon:1315	exon
CmaCh06G006710.1:exon:1316	CmaCh06G006710.1:exon:1316	exon
CmaCh06G006710.1:exon:1317	CmaCh06G006710.1:exon:1317	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh06G006710.1:five_prime_utr	CmaCh06G006710.1:five_prime_utr	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh06G006710.1:cds	CmaCh06G006710.1:cds	CDS
CmaCh06G006710.1:cds	CmaCh06G006710.1:cds_2	CDS
CmaCh06G006710.1:cds	CmaCh06G006710.1:cds_3	CDS
CmaCh06G006710.1:cds	CmaCh06G006710.1:cds_4	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CmaCh06G006710.1:three_prime_utr	CmaCh06G006710.1:three_prime_utr	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CmaCh06G006710.1	CmaCh06G006710.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006888	endoplasmic reticulum to Golgi vesicle-mediated transport
biological_process	GO:0007030	Golgi organization
biological_process	GO:0006886	intracellular protein transport
cellular_component	GO:0030134	COPII-coated ER to Golgi transport vesicle
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0005793	endoplasmic reticulum-Golgi intermediate compartment
cellular_component	GO:0005794	Golgi apparatus
cellular_component	GO:0016021	integral component of membrane