CmaCh06G006710 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh06G006710
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptiontransmembrane emp24 domain-containing protein p24beta2-like
LocationCma_Chr06: 3335327 .. 3336941 (+)
RNA-Seq ExpressionCmaCh06G006710
SyntenyCmaCh06G006710
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCATTCCTTGAATTCATTCGATTCTGTTCATTTGTGGAGTTCAAGAACAAAATGGAGATGGGAAGTGGGAAGTCAAAATACAGGTTTTTTATTTTCATGGCGGCTTTGTTCATATCATCACACCCAGTTCTGGGGATTAGATTTGTGATCGACAAGGAAGAATGCTTCTCTCACAATGTTGAATTCATCAGAGATACCATTTATCTCTCTTTTGTCGTCGTTAGCGTTAATACCTGGCGGCATCATGCTCACAATGGCGTTGATCTTTTGGTGGCCACTTCTTTCTCTCTCTCTCTCTCTCTCTTAGATTCATCCAGATTGTTCATATCATTTGCATGTTGTTTATGAGATGTTCGGCTCGTGAATTATCAGGGTTAAGACTTTGAATCTGATTTTACATACGTGTTGCTGTTACCTACCTTACAGAGGTCAAGGAACTGAGCCTTTTGTTTCGTATGTATGGATGAATGAGATTCACATTAGGGTTTAAATATATGTTGTCTATGAGATGTTCGGCTCGTGCATTATCAGGGTTAAGACTTTGAATCTGATTTTACATGCGTGTTGCTGTTACCTACCTTACAGAGGTCAAGGAACTGAGCCTTTTGTTTCGTATGTATGGATGAATGAGATTCACATTAGGGTTTGAATATATGTTGTTATAAGTTTTTTCAAGGTGTTTGTATTCGGTTTATGGATTAAGTTTTTGTACGACACAACAAGTTGTATACAAGTTTTTTTCTTATTTGAAGCTAGCTAGTGACTGTCAATTCCATGTGGATAGTAACATTTACATATCATATATAATACAAATCAATTTCATAAATCTTGTTTTAGGCTTGTTATGATTAAATGTGATTTGCAAAGTACAGGTGAAAGGGCCTTTAGGGGAGAGAATTGAAGAAATTAGAGGGAAAACAAGTGAGAAATTTGAGTTTATTTCTCTTAGAAAAGGGCGATATCATTTCTGCTTCATCAACAACTCACCCCACCAAGAGACCATAGAATTTGATATCCATATCAATCATTACTATGAGTTCTATGATGATGATGAACAACATGTAAAAGATGGTATTGATTCCTTTGATGAACCCTAAAGTTTTTACTTTGAAATTCATACTTCCCTTTACTTTTCTGCTCATCTCATCTGGTTTGAAATGAAATTTACAGAACATTTGAGCCCTTTATTGGCTCAAATAAAGAAGTTGGGAGAAACCTTGGGCGTTATTGAGTTTGAACAGCAATGGTTAGAGGCACAGACAGAGCGACAAGCCAAAGGTATTGTTCATTTATTAACCCATTTCATATACAATCTATTCCTTTGTATTAATTCAAATATCAGATCTAAACAATAACTTCTAGCATTTAGTAAGTAAGTGAAGTACCTTTTTTGCAGTGAATGAAGCAATGAGCACAATGGCGATTCTGAAGGCTGTTCTCGAATCAGCAGCACTCATCGGTGCAAGCAGTCTTCAAGTTTTCCTTCTGCGTCGCCTGTTCGAGAAAAAAGTTGGAAGCCTGTATTCGAGCACCTACAGAATATAATCTCATATCCGAACTCCAGCAGACATACAACAACAAGCATGGCAGAACAAGTGCTGGAAAGGTTGAGGAG

mRNA sequence

CCATTCCTTGAATTCATTCGATTCTGTTCATTTGTGGAGTTCAAGAACAAAATGGAGATGGGAAGTGGGAAGTCAAAATACAGGTTTTTTATTTTCATGGCGGCTTTGTTCATATCATCACACCCAGTTCTGGGGATTAGATTTGTGATCGACAAGGAAGAATGCTTCTCTCACAATGTTGAATTCATCAGAGATACCATTTATCTCTCTTTTGTCGTCGTTAGCGTTAATACCTGGCGGCATCATGCTCACAATGGCGTTGATCTTTTGGTGAAAGGGCCTTTAGGGGAGAGAATTGAAGAAATTAGAGGGAAAACAAGTGAGAAATTTGAGTTTATTTCTCTTAGAAAAGGGCGATATCATTTCTGCTTCATCAACAACTCACCCCACCAAGAGACCATAGAATTTGATATCCATATCAATCATTACTATGAGTTCTATGATGATGATGAACAACATGTAAAAGATGAACATTTGAGCCCTTTATTGGCTCAAATAAAGAAGTTGGGAGAAACCTTGGGCGTTATTGAGTTTGAACAGCAATGGTTAGAGGCACAGACAGAGCGACAAGCCAAAGTGAATGAAGCAATGAGCACAATGGCGATTCTGAAGGCTGTTCTCGAATCAGCAGCACTCATCGGTGCAAGCAGTCTTCAAGTTTTCCTTCTGCGTCGCCTGTTCGAGAAAAAAGTTGGAAGCCTGTATTCGAGCACCTACAGAATATAATCTCATATCCGAACTCCAGCAGACATACAACAACAAGCATGGCAGAACAAGTGCTGGAAAGGTTGAGGAG

Coding sequence (CDS)

ATGGAGATGGGAAGTGGGAAGTCAAAATACAGGTTTTTTATTTTCATGGCGGCTTTGTTCATATCATCACACCCAGTTCTGGGGATTAGATTTGTGATCGACAAGGAAGAATGCTTCTCTCACAATGTTGAATTCATCAGAGATACCATTTATCTCTCTTTTGTCGTCGTTAGCGTTAATACCTGGCGGCATCATGCTCACAATGGCGTTGATCTTTTGGTGAAAGGGCCTTTAGGGGAGAGAATTGAAGAAATTAGAGGGAAAACAAGTGAGAAATTTGAGTTTATTTCTCTTAGAAAAGGGCGATATCATTTCTGCTTCATCAACAACTCACCCCACCAAGAGACCATAGAATTTGATATCCATATCAATCATTACTATGAGTTCTATGATGATGATGAACAACATGTAAAAGATGAACATTTGAGCCCTTTATTGGCTCAAATAAAGAAGTTGGGAGAAACCTTGGGCGTTATTGAGTTTGAACAGCAATGGTTAGAGGCACAGACAGAGCGACAAGCCAAAGTGAATGAAGCAATGAGCACAATGGCGATTCTGAAGGCTGTTCTCGAATCAGCAGCACTCATCGGTGCAAGCAGTCTTCAAGTTTTCCTTCTGCGTCGCCTGTTCGAGAAAAAAGTTGGAAGCCTGTATTCGAGCACCTACAGAATATAA

Protein sequence

MEMGSGKSKYRFFIFMAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVNTWRHHAHNGVDLLVKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQHVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAALIGASSLQVFLLRRLFEKKVGSLYSSTYRI
Homology
BLAST of CmaCh06G006710 vs. ExPASy Swiss-Prot
Match: Q9S7M9 (Transmembrane emp24 domain-containing protein p24beta2 OS=Arabidopsis thaliana OX=3702 GN=At3g07680 PE=1 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 1.7e-53
Identity = 110/202 (54.46%), Postives = 143/202 (70.79%), Query Frame = 0

Query: 14  IFMAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVNTWRHHAHNGVDLL 73
           I +  L  S    LGIRFVID+EECFSH  E+  DT+++SFVV+  ++  H   +GVDL+
Sbjct: 7   IVLLGLLWSFQATLGIRFVIDREECFSHKAEYEGDTLHVSFVVIKSDSQWHFNEDGVDLV 66

Query: 74  VKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDD 133
           + GP GE+I + R + S K +F+  +KG Y FCF N SP+ ETI+FD+ + H + +YD  
Sbjct: 67  IHGPTGEQIHDFREQISAKHDFVVQKKGVYRFCFTNKSPYHETIDFDVQLGH-FAYYD-- 126

Query: 134 EQHVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESA 193
            QH KDEH +PL+ QI KL E L  I+FEQ WLEAQT+RQA VNE MS  A+ KA+ ES 
Sbjct: 127 -QHAKDEHFTPLMEQISKLEEALYNIQFEQHWLEAQTDRQAIVNENMSKRAVHKALFESF 186

Query: 194 ALIGASSLQVFLLRRLFEKKVG 216
           ALIGAS LQV+LLRRLFE+K+G
Sbjct: 187 ALIGASFLQVYLLRRLFERKLG 204

BLAST of CmaCh06G006710 vs. ExPASy Swiss-Prot
Match: Q9LIL4 (Transmembrane emp24 domain-containing protein p24beta3 OS=Arabidopsis thaliana OX=3702 GN=At3g22845 PE=2 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 4.1e-23
Identity = 67/209 (32.06%), Postives = 108/209 (51.67%), Query Frame = 0

Query: 7   KSKYRFFIFMAALFISS-HPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVNTWRHH 66
           ++K   F+ +  + ++S + +  +   ++ EEC    V +  DT+  +FVVV  + +   
Sbjct: 5   QAKIHVFVLIGLILLNSINQISSLSVTVNDEECVQEYVLYEGDTVSGNFVVVDHDIFWGS 64

Query: 67  AHNGVDLLVKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINH 126
            H G+D  V  P G  ++ ++G + +KFEF + + G Y FCF N     ET+ F IH+ H
Sbjct: 65  DHPGLDFTVTSPAGNIVQTLKGTSGDKFEFKAPKSGMYKFCFHNPYSTPETVSFYIHVGH 124

Query: 127 YYEFYDDDEQHVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAI 186
                 ++    KDEHL P+  +I +L E L  +  EQ++L+A+  R    NE+     I
Sbjct: 125 ----IPNEHDLAKDEHLDPVNVKIAELREALESVVAEQKYLKARDTRHRHTNESTRKRVI 184

Query: 187 LKAVLESAALIGASSLQVFLLRRLFEKKV 215
              V E   L  AS LQV  +R+LF K V
Sbjct: 185 FYTVGEYIFLAAASGLQVLYIRKLFSKSV 209

BLAST of CmaCh06G006710 vs. ExPASy TrEMBL
Match: A0A6J1KYW1 (transmembrane emp24 domain-containing protein p24beta2-like OS=Cucurbita maxima OX=3661 GN=LOC111498823 PE=3 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 6.5e-112
Identity = 209/209 (100.00%), Postives = 209/209 (100.00%), Query Frame = 0

Query: 16  MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVNTWRHHAHNGVDLLVK 75
           MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVNTWRHHAHNGVDLLVK
Sbjct: 1   MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVNTWRHHAHNGVDLLVK 60

Query: 76  GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ 135
           GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ
Sbjct: 61  GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ 120

Query: 136 HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAAL 195
           HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAAL
Sbjct: 121 HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAAL 180

Query: 196 IGASSLQVFLLRRLFEKKVGSLYSSTYRI 225
           IGASSLQVFLLRRLFEKKVGSLYSSTYRI
Sbjct: 181 IGASSLQVFLLRRLFEKKVGSLYSSTYRI 209

BLAST of CmaCh06G006710 vs. ExPASy TrEMBL
Match: A0A6J1GJG3 (transmembrane emp24 domain-containing protein p24beta2-like OS=Cucurbita moschata OX=3662 GN=LOC111454883 PE=3 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 2.5e-111
Identity = 208/209 (99.52%), Postives = 209/209 (100.00%), Query Frame = 0

Query: 16  MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVNTWRHHAHNGVDLLVK 75
           MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSV+TWRHHAHNGVDLLVK
Sbjct: 1   MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVHTWRHHAHNGVDLLVK 60

Query: 76  GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ 135
           GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ
Sbjct: 61  GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ 120

Query: 136 HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAAL 195
           HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAAL
Sbjct: 121 HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAAL 180

Query: 196 IGASSLQVFLLRRLFEKKVGSLYSSTYRI 225
           IGASSLQVFLLRRLFEKKVGSLYSSTYRI
Sbjct: 181 IGASSLQVFLLRRLFEKKVGSLYSSTYRI 209

BLAST of CmaCh06G006710 vs. ExPASy TrEMBL
Match: A0A6J1CS53 (transmembrane emp24 domain-containing protein p24beta2-like OS=Momordica charantia OX=3673 GN=LOC111013807 PE=3 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 1.3e-72
Identity = 150/224 (66.96%), Postives = 174/224 (77.68%), Query Frame = 0

Query: 1   MEMGSGKSKYRFFIFMAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVN 60
           M +G  K   R  +    + +S+  V G RFVIDKEECFSHNVEF RDTI+LSFVVVSV+
Sbjct: 1   MRIGKSKGWLRIAMVGLLVSVSAEAVFGFRFVIDKEECFSHNVEFTRDTIHLSFVVVSVH 60

Query: 61  TWRHHAHNGVDLLVKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFD 120
               HAHNGVDL VKGP GE IEE +GKTSEKF+F++  KG Y FCF N SPH ETI+FD
Sbjct: 61  ASWRHAHNGVDLSVKGPKGEGIEEHKGKTSEKFKFVAHFKGPYRFCFTNKSPHHETIDFD 120

Query: 121 IHINHYYEFYDDDEQHVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAM 180
           IHI  Y+E YD D QHV++EHLSPL+ QI+KL E L ++EFEQQWLEAQTERQ KVNEAM
Sbjct: 121 IHITRYFEPYDHD-QHVQNEHLSPLMDQIEKLREALDIVEFEQQWLEAQTERQVKVNEAM 180

Query: 181 STMAILKAVLESAALIGASSLQVFLLRRLFEKKVGSLYSSTYRI 225
           S  AILKA+LESAALIGAS LQV LL RLFEK+VG L+S++Y I
Sbjct: 181 SRWAILKALLESAALIGASGLQVCLLCRLFEKRVGVLHSNSYTI 223

BLAST of CmaCh06G006710 vs. ExPASy TrEMBL
Match: A0A6J5XME6 (GOLD domain-containing protein OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCUS34477 PE=3 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 7.5e-60
Identity = 121/202 (59.90%), Postives = 154/202 (76.24%), Query Frame = 0

Query: 14  IFMAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVNTWRHHAHNGVDLL 73
           + +    +S   V GIRFVID+EECFSHNV+F  DT+++SFVV+ V+   H+  +GVDL+
Sbjct: 11  VLLLTFLVSLQAVFGIRFVIDREECFSHNVQFEGDTVHISFVVIKVDASWHYTQDGVDLV 70

Query: 74  VKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDD 133
           VKGP GE+I + R KTSEKF+F++  KG + FCF N SP+ ETI+FDIH++H + +YD  
Sbjct: 71  VKGPSGEQIHDFRDKTSEKFDFVTRHKGVHQFCFTNKSPYHETIDFDIHVSH-FTYYD-- 130

Query: 134 EQHVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESA 193
            QH KDEH +PLL Q+ KL E L  I+FEQ WLEAQTERQA VNEAMS  AI KA  ESA
Sbjct: 131 -QHAKDEHFNPLLEQVSKLEEALYNIQFEQHWLEAQTERQAIVNEAMSRRAIHKAFFESA 190

Query: 194 ALIGASSLQVFLLRRLFEKKVG 216
           AL+GAS+LQV+LLRRLFE+K+G
Sbjct: 191 ALVGASALQVYLLRRLFERKLG 208

BLAST of CmaCh06G006710 vs. ExPASy TrEMBL
Match: M5WCT6 (GOLD domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_6G024700 PE=3 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 1.7e-59
Identity = 120/202 (59.41%), Postives = 154/202 (76.24%), Query Frame = 0

Query: 14  IFMAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVNTWRHHAHNGVDLL 73
           + +    +S   V GIRFVID+EECFSHNV++  DT+++SFVV+ V+   H+  +GVDL+
Sbjct: 11  VLLLTFLVSLQAVFGIRFVIDREECFSHNVQYEGDTVHISFVVIKVDASWHYTQDGVDLV 70

Query: 74  VKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDD 133
           VKGP GE+I + R KTSEKF+F++  KG + FCF N SP+ ETI+FDIH++H + +YD  
Sbjct: 71  VKGPSGEQIHDFRDKTSEKFDFVTRHKGVHQFCFTNKSPYHETIDFDIHVSH-FTYYD-- 130

Query: 134 EQHVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESA 193
            QH KDEH +PLL Q+ KL E L  I+FEQ WLEAQTERQA VNEAMS  AI KA  ESA
Sbjct: 131 -QHAKDEHFNPLLEQVSKLEEALYNIQFEQHWLEAQTERQAIVNEAMSRRAIHKAFFESA 190

Query: 194 ALIGASSLQVFLLRRLFEKKVG 216
           AL+GAS+LQV+LLRRLFE+K+G
Sbjct: 191 ALVGASALQVYLLRRLFERKLG 208

BLAST of CmaCh06G006710 vs. NCBI nr
Match: KAG6596756.1 (Transmembrane emp24 domain-containing protein p24beta2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028293.1 Transmembrane emp24 domain-containing protein p24beta2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 441.0 bits (1133), Expect = 6.0e-120
Identity = 223/224 (99.55%), Postives = 224/224 (100.00%), Query Frame = 0

Query: 1   MEMGSGKSKYRFFIFMAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVN 60
           MEMGSGKSKYRFFIFMAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSV+
Sbjct: 1   MEMGSGKSKYRFFIFMAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVH 60

Query: 61  TWRHHAHNGVDLLVKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFD 120
           TWRHHAHNGVDLLVKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFD
Sbjct: 61  TWRHHAHNGVDLLVKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFD 120

Query: 121 IHINHYYEFYDDDEQHVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAM 180
           IHINHYYEFYDDDEQHVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAM
Sbjct: 121 IHINHYYEFYDDDEQHVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAM 180

Query: 181 STMAILKAVLESAALIGASSLQVFLLRRLFEKKVGSLYSSTYRI 225
           STMAILKAVLESAALIGASSLQVFLLRRLFEKKVGSLYSSTYRI
Sbjct: 181 STMAILKAVLESAALIGASSLQVFLLRRLFEKKVGSLYSSTYRI 224

BLAST of CmaCh06G006710 vs. NCBI nr
Match: XP_023005965.1 (transmembrane emp24 domain-containing protein p24beta2-like [Cucurbita maxima])

HSP 1 Score: 413.3 bits (1061), Expect = 1.3e-111
Identity = 209/209 (100.00%), Postives = 209/209 (100.00%), Query Frame = 0

Query: 16  MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVNTWRHHAHNGVDLLVK 75
           MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVNTWRHHAHNGVDLLVK
Sbjct: 1   MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVNTWRHHAHNGVDLLVK 60

Query: 76  GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ 135
           GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ
Sbjct: 61  GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ 120

Query: 136 HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAAL 195
           HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAAL
Sbjct: 121 HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAAL 180

Query: 196 IGASSLQVFLLRRLFEKKVGSLYSSTYRI 225
           IGASSLQVFLLRRLFEKKVGSLYSSTYRI
Sbjct: 181 IGASSLQVFLLRRLFEKKVGSLYSSTYRI 209

BLAST of CmaCh06G006710 vs. NCBI nr
Match: XP_022952151.1 (transmembrane emp24 domain-containing protein p24beta2-like [Cucurbita moschata])

HSP 1 Score: 411.4 bits (1056), Expect = 5.1e-111
Identity = 208/209 (99.52%), Postives = 209/209 (100.00%), Query Frame = 0

Query: 16  MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVNTWRHHAHNGVDLLVK 75
           MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSV+TWRHHAHNGVDLLVK
Sbjct: 1   MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVHTWRHHAHNGVDLLVK 60

Query: 76  GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ 135
           GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ
Sbjct: 61  GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ 120

Query: 136 HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAAL 195
           HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAAL
Sbjct: 121 HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESAAL 180

Query: 196 IGASSLQVFLLRRLFEKKVGSLYSSTYRI 225
           IGASSLQVFLLRRLFEKKVGSLYSSTYRI
Sbjct: 181 IGASSLQVFLLRRLFEKKVGSLYSSTYRI 209

BLAST of CmaCh06G006710 vs. NCBI nr
Match: XP_023539502.1 (transmembrane emp24 domain-containing protein p24beta2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 328.6 bits (841), Expect = 4.3e-86
Identity = 159/160 (99.38%), Postives = 160/160 (100.00%), Query Frame = 0

Query: 16  MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVNTWRHHAHNGVDLLVK 75
           MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSV+TWRHHAHNGVDLLVK
Sbjct: 1   MAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVHTWRHHAHNGVDLLVK 60

Query: 76  GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ 135
           GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ
Sbjct: 61  GPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDDEQ 120

Query: 136 HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAK 176
           HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAK
Sbjct: 121 HVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAK 160

BLAST of CmaCh06G006710 vs. NCBI nr
Match: XP_022144018.1 (transmembrane emp24 domain-containing protein p24beta2-like [Momordica charantia])

HSP 1 Score: 282.7 bits (722), Expect = 2.7e-72
Identity = 150/224 (66.96%), Postives = 174/224 (77.68%), Query Frame = 0

Query: 1   MEMGSGKSKYRFFIFMAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVN 60
           M +G  K   R  +    + +S+  V G RFVIDKEECFSHNVEF RDTI+LSFVVVSV+
Sbjct: 1   MRIGKSKGWLRIAMVGLLVSVSAEAVFGFRFVIDKEECFSHNVEFTRDTIHLSFVVVSVH 60

Query: 61  TWRHHAHNGVDLLVKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFD 120
               HAHNGVDL VKGP GE IEE +GKTSEKF+F++  KG Y FCF N SPH ETI+FD
Sbjct: 61  ASWRHAHNGVDLSVKGPKGEGIEEHKGKTSEKFKFVAHFKGPYRFCFTNKSPHHETIDFD 120

Query: 121 IHINHYYEFYDDDEQHVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAM 180
           IHI  Y+E YD D QHV++EHLSPL+ QI+KL E L ++EFEQQWLEAQTERQ KVNEAM
Sbjct: 121 IHITRYFEPYDHD-QHVQNEHLSPLMDQIEKLREALDIVEFEQQWLEAQTERQVKVNEAM 180

Query: 181 STMAILKAVLESAALIGASSLQVFLLRRLFEKKVGSLYSSTYRI 225
           S  AILKA+LESAALIGAS LQV LL RLFEK+VG L+S++Y I
Sbjct: 181 SRWAILKALLESAALIGASGLQVCLLCRLFEKRVGVLHSNSYTI 223

BLAST of CmaCh06G006710 vs. TAIR 10
Match: AT3G07680.1 (emp24/gp25L/p24 family/GOLD family protein )

HSP 1 Score: 210.7 bits (535), Expect = 1.2e-54
Identity = 110/202 (54.46%), Postives = 143/202 (70.79%), Query Frame = 0

Query: 14  IFMAALFISSHPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVNTWRHHAHNGVDLL 73
           I +  L  S    LGIRFVID+EECFSH  E+  DT+++SFVV+  ++  H   +GVDL+
Sbjct: 7   IVLLGLLWSFQATLGIRFVIDREECFSHKAEYEGDTLHVSFVVIKSDSQWHFNEDGVDLV 66

Query: 74  VKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINHYYEFYDDD 133
           + GP GE+I + R + S K +F+  +KG Y FCF N SP+ ETI+FD+ + H + +YD  
Sbjct: 67  IHGPTGEQIHDFREQISAKHDFVVQKKGVYRFCFTNKSPYHETIDFDVQLGH-FAYYD-- 126

Query: 134 EQHVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAILKAVLESA 193
            QH KDEH +PL+ QI KL E L  I+FEQ WLEAQT+RQA VNE MS  A+ KA+ ES 
Sbjct: 127 -QHAKDEHFTPLMEQISKLEEALYNIQFEQHWLEAQTDRQAIVNENMSKRAVHKALFESF 186

Query: 194 ALIGASSLQVFLLRRLFEKKVG 216
           ALIGAS LQV+LLRRLFE+K+G
Sbjct: 187 ALIGASFLQVYLLRRLFERKLG 204

BLAST of CmaCh06G006710 vs. TAIR 10
Match: AT3G22845.1 (emp24/gp25L/p24 family/GOLD family protein )

HSP 1 Score: 109.8 bits (273), Expect = 2.9e-24
Identity = 67/209 (32.06%), Postives = 108/209 (51.67%), Query Frame = 0

Query: 7   KSKYRFFIFMAALFISS-HPVLGIRFVIDKEECFSHNVEFIRDTIYLSFVVVSVNTWRHH 66
           ++K   F+ +  + ++S + +  +   ++ EEC    V +  DT+  +FVVV  + +   
Sbjct: 5   QAKIHVFVLIGLILLNSINQISSLSVTVNDEECVQEYVLYEGDTVSGNFVVVDHDIFWGS 64

Query: 67  AHNGVDLLVKGPLGERIEEIRGKTSEKFEFISLRKGRYHFCFINNSPHQETIEFDIHINH 126
            H G+D  V  P G  ++ ++G + +KFEF + + G Y FCF N     ET+ F IH+ H
Sbjct: 65  DHPGLDFTVTSPAGNIVQTLKGTSGDKFEFKAPKSGMYKFCFHNPYSTPETVSFYIHVGH 124

Query: 127 YYEFYDDDEQHVKDEHLSPLLAQIKKLGETLGVIEFEQQWLEAQTERQAKVNEAMSTMAI 186
                 ++    KDEHL P+  +I +L E L  +  EQ++L+A+  R    NE+     I
Sbjct: 125 ----IPNEHDLAKDEHLDPVNVKIAELREALESVVAEQKYLKARDTRHRHTNESTRKRVI 184

Query: 187 LKAVLESAALIGASSLQVFLLRRLFEKKV 215
              V E   L  AS LQV  +R+LF K V
Sbjct: 185 FYTVGEYIFLAAASGLQVLYIRKLFSKSV 209

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9S7M91.7e-5354.46Transmembrane emp24 domain-containing protein p24beta2 OS=Arabidopsis thaliana O... [more]
Q9LIL44.1e-2332.06Transmembrane emp24 domain-containing protein p24beta3 OS=Arabidopsis thaliana O... [more]
Match NameE-valueIdentityDescription
A0A6J1KYW16.5e-112100.00transmembrane emp24 domain-containing protein p24beta2-like OS=Cucurbita maxima ... [more]
A0A6J1GJG32.5e-11199.52transmembrane emp24 domain-containing protein p24beta2-like OS=Cucurbita moschat... [more]
A0A6J1CS531.3e-7266.96transmembrane emp24 domain-containing protein p24beta2-like OS=Momordica charant... [more]
A0A6J5XME67.5e-6059.90GOLD domain-containing protein OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCUS34... [more]
M5WCT61.7e-5959.41GOLD domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_6G024700 PE=3 ... [more]
Match NameE-valueIdentityDescription
KAG6596756.16.0e-12099.55Transmembrane emp24 domain-containing protein p24beta2, partial [Cucurbita argyr... [more]
XP_023005965.11.3e-111100.00transmembrane emp24 domain-containing protein p24beta2-like [Cucurbita maxima][more]
XP_022952151.15.1e-11199.52transmembrane emp24 domain-containing protein p24beta2-like [Cucurbita moschata][more]
XP_023539502.14.3e-8699.38transmembrane emp24 domain-containing protein p24beta2-like [Cucurbita pepo subs... [more]
XP_022144018.12.7e-7266.96transmembrane emp24 domain-containing protein p24beta2-like [Momordica charantia... [more]
Match NameE-valueIdentityDescription
AT3G07680.11.2e-5454.46emp24/gp25L/p24 family/GOLD family protein [more]
AT3G22845.12.9e-2432.06emp24/gp25L/p24 family/GOLD family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR009038GOLD domainSMARTSM01190EMP24_GP25L_2coord: 28..211
e-value: 7.2E-31
score: 118.6
IPR009038GOLD domainPFAMPF01105EMP24_GP25Lcoord: 35..211
e-value: 4.3E-27
score: 95.2
IPR009038GOLD domainPROSITEPS50866GOLDcoord: 36..123
score: 11.675441
NoneNo IPR availablePANTHERPTHR22811:SF167TMP21-RELATED PROTEIN-RELATEDcoord: 25..215
IPR015720Transmembrane emp24 domain-containing proteinPANTHERPTHR22811TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEINcoord: 25..215

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh06G006710.1CmaCh06G006710.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006888 endoplasmic reticulum to Golgi vesicle-mediated transport
biological_process GO:0007030 Golgi organization
biological_process GO:0006886 intracellular protein transport
cellular_component GO:0030134 COPII-coated ER to Golgi transport vesicle
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0005793 endoplasmic reticulum-Golgi intermediate compartment
cellular_component GO:0005794 Golgi apparatus
cellular_component GO:0016021 integral component of membrane