CmaCh03G003810.1 (mRNA) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh03G003810.1 vs. ExPASy Swiss-Prot
Match: Q9LFD2 (Laccase-8 OS=Arabidopsis thaliana OX=3702 GN=LAC8 PE=2 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 3.3e-14
Identity = 83/304 (27.30%), Postives = 133/304 (43.75%), Query Frame = 0

Query: 3   VEDMTVRRLSRDLVITAVNGEYPGPTIDVQDDDVLIVHLSNNSPYDLTIHCFLYH--FQA 62
           ++D+ V+ L ++ +I A NG  PGPTI+V++ D L+V++ NNS Y++TIH   +H  FQ 
Sbjct: 34  IQDVVVKPLCKEQIIPAANGSLPGPTINVREGDTLVVNVINNSTYNVTIH---WHGVFQL 93

Query: 63  WSIPVAKRVGRRSGKYNPMSDTARWKLHVQIQYQRIRRNFMVACPLVLATRH-----RPR 122
            S+ +    G       P+     +     I  Q     +      + AT H     RP 
Sbjct: 94  KSVWMD---GANMITQCPIQPGYNFTYQFDITGQEGTLLWHAHVVNLRATLHGALVIRP- 153

Query: 123 RSSHPPPSPAYRCCTQSPTKRFQYCWVSGGMPTLSTLKRKASPPAAVL----MAPTPTPL 182
           RS  P P P        P K     +       +  L+ + +P +       +A    P 
Sbjct: 154 RSGRPYPFP-------KPYKEVPIVFQQWWDTDVRLLQLRPAPVSDAYLINGLAGDSYPC 213

Query: 183 MDSP--ETSTLAPKDLL--------------------ASIVAVDATYTVPYVTDVIVLAP 242
            ++       +  K  L                     ++VAVDA Y+ PY+TDV++L P
Sbjct: 214 SENRMFNLKVVQGKTYLLRIVNAALNTHLFFKIANHNVTVVAVDAVYSTPYLTDVMILTP 273

Query: 243 GQTTDVLVNADQPLGSYYMAARVWLMHCHL---DVHLPWGLAMGFEVENGPTPSTRLPPP 271
           GQT D L+ ADQ +G YYMA   ++    +   D+    GL +     +  +P+  L P 
Sbjct: 274 GQTVDALLTADQAIGKYYMATLPYISAIGIPTPDIKPTRGLIVYQGATSSSSPAEPLMPV 323

BLAST of CmaCh03G003810.1 vs. ExPASy Swiss-Prot
Match: Q9LFD1 (Laccase-9 OS=Arabidopsis thaliana OX=3702 GN=LAC9 PE=2 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 9.5e-14
Identity = 85/306 (27.78%), Postives = 127/306 (41.50%), Query Frame = 0

Query: 3   VEDMTVRRLSRDLVITAVNGEYPGPTIDVQDDDVLIVHLSNNSPYDLTIHCFLYH--FQA 62
           V+D+ V  L ++ +I  VNG  PGPTI+V++ D L+VH+ N S Y++TIH   +H  FQ 
Sbjct: 34  VKDVVVTPLCKEQMIPIVNGSLPGPTINVREGDTLVVHVINKSTYNVTIH---WHGVFQL 93

Query: 63  WSIPVAKRVGRRSGKYNPMSDTARWKLHVQIQYQRIRRNFMVACPLVLATRH-----RPR 122
            S+ +    G       P+  +  +     I  Q     +      + AT H     RP 
Sbjct: 94  KSVWMD---GANMITQCPIQPSNNFTYQFDITGQEGTLLWHAHVVNLRATIHGALIIRP- 153

Query: 123 RSSHPPPSPAYRCCTQSPTKRFQYCWVSGGMPTLSTLKRKASPPAAVLMA------PTPT 182
           RS  P P P        P K     +       +  L+ + +P +   +         P 
Sbjct: 154 RSGRPYPFP-------KPYKEVPLIFQQWWDTDVRLLELRPAPVSDAYLINGLAGDSYPC 213

Query: 183 PLMDSPETSTLAPKDLL--------------------ASIVAVDATYTVPYVTDVIVLAP 242
                     +  K  L                     ++VAVDA YT PY+TDV++L P
Sbjct: 214 SKNRMFNLKVVQGKTYLLRIINAALNTHLFFKIANHNVTVVAVDAVYTTPYLTDVMILTP 273

Query: 243 GQTTDVLVNADQPLGSYYMAARVWLMHCHLDVHLPWGLAMGFEVENGPT----PSTRLPP 272
           GQT D ++ ADQP+G+YYMA   +     +          G  V  G T    P+    P
Sbjct: 274 GQTIDAILTADQPIGTYYMAIIPYFSAIGVPASPDTKPTRGLIVYEGATSSSSPTKPWMP 325

BLAST of CmaCh03G003810.1 vs. ExPASy Swiss-Prot
Match: Q69L99 (Laccase-14 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC14 PE=3 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 1.2e-13
Identity = 36/75 (48.00%), Postives = 45/75 (60.00%), Query Frame = 0

Query: 199 PYVTDVIVLAPGQTTDVLVNADQPLGSYYMAARVWLMHCHLDVHLPWGLAMGFEVENGPT 258
           P   + I + PG    +   AD P         VWLMHCHL+ HLP+GLAM F+V++GPT
Sbjct: 517 PQQRNTIAVPPGGWAVIRFTADNP--------GVWLMHCHLEAHLPFGLAMAFDVQDGPT 576

Query: 259 PSTRLPPPPHDLPKC 274
           P   LPPPP+D P C
Sbjct: 577 PDAMLPPPPNDYPPC 583

HSP 2 Score: 76.6 bits (187), Expect = 4.7e-13
Identity = 80/273 (29.30%), Postives = 123/273 (45.05%), Query Frame = 0

Query: 3   VEDMTVRRLSRDLVITAVNGEYPGPTIDVQDDDVLIVHLSNNSPYDLTIHC--FLYHFQA 62
           V ++TV RL +  VITAVNG++PGP ++ ++ D L+V + NNSPY++TIH    L    A
Sbjct: 39  VGNLTVERLGQRQVITAVNGQFPGPKVEARNGDTLLVRVVNNSPYNITIHWHGVLQRLSA 98

Query: 63  WS--------IPVAKRVGRRSG---KYN--PMSDTARWKLHVQIQYQRIRRNFMVACPLV 122
           W+         P+    G  S    ++N      T  W  HV      +    ++     
Sbjct: 99  WADGPAMVTQCPILPGSGAGSSYTYRFNVTGQEGTLWWHAHVSFLRATVYGALLI----- 158

Query: 123 LATRHRPRRS-SHPPPSP---------AYRCCTQSPTKRFQYCWVSGGMPTLSTLKRKAS 182
                RPR    +P P+P          +   + +     +  +++GG P  S       
Sbjct: 159 -----RPRPGVPYPFPAPHAEHTLLLGEWWNASATLVDVERQAFLTGGQPANSVALTING 218

Query: 183 PPAA---------VLMAPTPTPLMDSPETS-------TLAPKDLLASIVAVDATYTVPYV 234
            P           + +A   T L+     +        +A  +   ++VAVDA YT PY 
Sbjct: 219 MPGLSHAHKEMHHLRVARGNTYLLRLVNAALNYQLFFKVAAHNF--TVVAVDACYTDPYH 278

BLAST of CmaCh03G003810.1 vs. ExPASy Swiss-Prot
Match: Q2QYS3 (Laccase-23 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC23 PE=3 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 2.8e-13
Identity = 77/266 (28.95%), Postives = 117/266 (43.98%), Query Frame = 0

Query: 3   VEDMTVRRLSRDLVITAVNGEYPGPTIDVQDDDVLIVHLSNNSPYDLTIHCFLYHFQAWS 62
           + + TV+RL +   I  VNG++PGPT+++++ D LI++L N   Y++T+H        W 
Sbjct: 31  IRETTVKRLCKSQSIMTVNGQFPGPTLEIKEGDSLIINLINRGRYNVTLH--------WH 90

Query: 63  IPVAKRVGRRSG-KYNPM-----SDTARWKLHVQIQYQRI---RRNFMVACPLVLATRHR 122
                R G   G +Y          + R++  V  Q   +     +  +   +  A   R
Sbjct: 91  GVRQMRTGWSDGPEYVTQCPVRPGQSYRYRFTVAAQEGTLWWHAHSSWLRATVYGALLIR 150

Query: 123 PRRSSHPP----------PSPAYRCCTQSPTKRFQYCWVSGGMPTLS-TLKRKASPPAAV 182
           PR  +  P          P         +P    +    +G  P +S  L   A P    
Sbjct: 151 PRDGTSYPFHVQPTRELAPILLGEWWDMNPVDVVRAATRTGAAPNISDALTVNAQPGDLY 210

Query: 183 LMAPTPTPL--MDSPETSTL------APKDLLAS-------IVAVDATYTVPYVTDVIVL 234
             +   T    + S ET+ L         +L  S       +VA DA+YT PY T +++L
Sbjct: 211 SCSSHDTAFFPVTSGETNLLRFINAALNTELFVSLAGHNMTVVAADASYTKPYTTSLLLL 270

HSP 2 Score: 60.5 bits (145), Expect = 3.5e-08
Identity = 26/42 (61.90%), Postives = 29/42 (69.05%), Query Frame = 0

Query: 232 VWLMHCHLDVHLPWGLAMGFEVENGPTPSTRLPPPPHDLPKC 274
           VWLMHCHLDVH+ WGLAM F V++G      L  PP DLP C
Sbjct: 526 VWLMHCHLDVHITWGLAMAFLVDDGVGELQSLEAPPPDLPLC 567

BLAST of CmaCh03G003810.1 vs. ExPASy Swiss-Prot
Match: Q2RBK2 (Putative laccase-17 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC17 PE=3 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 3.6e-13
Identity = 77/266 (28.95%), Postives = 117/266 (43.98%), Query Frame = 0

Query: 3   VEDMTVRRLSRDLVITAVNGEYPGPTIDVQDDDVLIVHLSNNSPYDLTIHCFLYHFQAWS 62
           + + TV+RL +   I  VNG++PGPT+++ + D LI++L N   Y++T+H        W 
Sbjct: 31  IRETTVKRLCKSHNIMTVNGQFPGPTLEINEGDSLIINLINRGRYNMTLH--------WH 90

Query: 63  IPVAKRVGRRSG-KYNPM-----SDTARWKLHVQIQYQRI---RRNFMVACPLVLATRHR 122
                R G   G +Y          + R++  V  Q   +     +  +   +  A   R
Sbjct: 91  GVRQMRTGWSDGPEYVTQCPVRPGQSYRYRFTVAAQEGTLWWHAHSSWLRATVYGALLIR 150

Query: 123 PRRSSHPP----------PSPAYRCCTQSPTKRFQYCWVSGGMPTLS-TLKRKASPPAAV 182
           PR  +  P          P         +P    +    +G  P +S  L   A P    
Sbjct: 151 PRDGTSYPFDVQPTRELAPILLGEWWDMNPVDVVRAATRTGAAPNISDALTVNAQPGDLY 210

Query: 183 LMAPTPTPL--MDSPETSTL------APKDLLAS-------IVAVDATYTVPYVTDVIVL 234
             +   T +  + S ET+ L         +L  S       +VA DA+YT PY T +++L
Sbjct: 211 SCSSHDTAVFPVTSGETNLLRFINAALNTELFVSLAGHNMTVVAADASYTKPYTTSLLLL 270

HSP 2 Score: 60.5 bits (145), Expect = 3.5e-08
Identity = 26/42 (61.90%), Postives = 29/42 (69.05%), Query Frame = 0

Query: 232 VWLMHCHLDVHLPWGLAMGFEVENGPTPSTRLPPPPHDLPKC 274
           VWLMHCHLDVH+ WGLAM F V++G      L  PP DLP C
Sbjct: 526 VWLMHCHLDVHITWGLAMAFLVDDGVGELQSLEAPPPDLPLC 567

BLAST of CmaCh03G003810.1 vs. ExPASy TrEMBL
Match: A0A6J1GEQ7 (Laccase OS=Cucurbita moschata OX=3662 GN=LOC111453486 PE=3 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 1.2e-30
Identity = 126/304 (41.45%), Postives = 150/304 (49.34%), Query Frame = 0

Query: 2   TVEDMTVRRLSRDLVITAVNGEYPGPTIDVQDDDVLIVHLSNNSPYDLTIHCFLYH--FQ 61
           TVEDMTVRRL RD VITAVNGEYPGPTI VQDDDVLIVH+SNNSPYDLTIH   +H  FQ
Sbjct: 29  TVEDMTVRRLCRDQVITAVNGEYPGPTIHVQDDDVLIVHVSNNSPYDLTIH---WHGVFQ 88

Query: 62  AWSIPVAKRVGRRSGKYNPMSDTARWKLHVQIQYQRIRRNFMVACPLVLATRH-----RP 121
             S   A   G  +    P+     +     I+ Q     +      + AT H     RP
Sbjct: 89  LLS---AWADGPENVTQCPIRPGGNYTYRFNIKGQEGTLWWHAHSSWLRATVHGALLIRP 148

Query: 122 RRSSHPPPSPAYRCCTQSPTKRFQYC---WVSGGMPTLS----TLKRKASPPAAVLMAPT 181
            +S  P P        Q P K+       W +  +  +        R  +   A  +   
Sbjct: 149 -KSGLPLP-------YQKPYKKVPILLGEWWNANVVHVEEEGLAAGRGPNGSDAYTINGL 208

Query: 182 PTPLMDSPETSTLAPK------DLL-------------------ASIVAVDATYTVPYVT 241
           P  L   P+  T   K       LL                    ++VAVDATYTVPYVT
Sbjct: 209 PGNLYPCPQNQTYQLKMARGKTSLLQVVNAALNNQLFFKLANHNLTVVAVDATYTVPYVT 268

Query: 242 DVIVLAPGQTTDVLVNADQPLGSYYMAARVWLMHCHLDVHLPWGLAMGFEVENG-PTPST 266
           DVIVLAPGQTTDVLVNADQPLGSYYMAAR +       +  P  +     + +G PT ST
Sbjct: 269 DVIVLAPGQTTDVLVNADQPLGSYYMAARPY-ADTRPQIAFPNTITRAIVIYDGAPTNST 317

BLAST of CmaCh03G003810.1 vs. ExPASy TrEMBL
Match: A0A6J1GEQ7 (Laccase OS=Cucurbita moschata OX=3662 GN=LOC111453486 PE=3 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 2.5e-17
Identity = 41/42 (97.62%), Postives = 41/42 (97.62%), Query Frame = 0

Query: 232 VWLMHCHLDVHLPWGLAMGFEVENGPTPSTRLPPPPHDLPKC 274
           VWLMHCHLDVHLPWGLAMGFEV NGPTPSTRLPPPPHDLPKC
Sbjct: 527 VWLMHCHLDVHLPWGLAMGFEVANGPTPSTRLPPPPHDLPKC 568

HSP 2 Score: 138.7 bits (348), Expect = 3.7e-29
Identity = 107/329 (32.52%), Postives = 144/329 (43.77%), Query Frame = 0

Query: 3   VEDMTVRRLSRDLVITAVNGEYPGPTIDVQDDDVLIVHLSNNSPYDLTIHC--FLYHFQA 62
           VED+  + L R  VITAVNG  PGPTI+ ++ D +I+H+ N SPY+LTIH    +     
Sbjct: 1   VEDIDFQLLCRPQVITAVNGTLPGPTINAREGDTVIIHVFNKSPYNLTIHWHGIIQFLTP 60

Query: 63  WS--------IPVAKRVGRRSGKYN--PMSDTARWKLHVQIQYQRIRRNFMV-------- 122
           WS         P+    G  + ++N      T  W  H       +    ++        
Sbjct: 61  WSDGPEYVTQCPIPSN-GSYTYQFNLTGQEGTLWWHAHSSFLRATVYGALIIKPRLGHSY 120

Query: 123 -------ACPLVLA---------TRHRPRRSSHPPPSPAYRCCTQSPTKRFQYCWVSGGM 182
                    PLV+                 + +PP           P   F     S   
Sbjct: 121 PYPKVYQEVPLVVGEWWNANVEEVERNATDTGNPPLESIASTINGFPGDLFN---CSQDK 180

Query: 183 PTLSTLKRKASPPAAVLMAPTPTPLMDSPETSTLAPKDLLASIVAVDATYTVPYVTDVIV 242
             +  +KR  +    V+ A      ++      +A   L  ++VA+DA YT  Y TDVIV
Sbjct: 181 TYMLKVKRGKTYLLRVINA-----ALNEQHFFKVANHSL--TVVAMDAIYTEHYNTDVIV 240

Query: 243 LAPGQTTDVLVNADQPLGSYYMAAR----------------------VWLMHCHLDVHLP 274
           LAPGQT DVL+  +Q +GSYYM                         +W MHCHL+ HLP
Sbjct: 241 LAPGQTVDVLLRTNQAVGSYYMVFTPYRSSSVGTNNVTTRGVIVYDGIWFMHCHLETHLP 300

BLAST of CmaCh03G003810.1 vs. ExPASy TrEMBL
Match: A0A1S3BPD5 (Laccase OS=Cucumis melo OX=3656 GN=LOC103491871 PE=3 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 6.6e-26
Identity = 107/294 (36.39%), Postives = 140/294 (47.62%), Query Frame = 0

Query: 2   TVEDMTVRRLSRDLVITAVNGEYPGPTIDVQDDDVLIVHLSNNSPYDLTIHCFLYHFQAW 61
           TVEDM+VRRL RD VITAVNGEYPGPTI VQD+DVL+VH++NNSP+DLTIH        W
Sbjct: 33  TVEDMSVRRLCRDQVITAVNGEYPGPTIHVQDEDVLVVHVTNNSPHDLTIH--------W 92

Query: 62  SIPVAKRVGRRSGKYN----PMSDTARWKLHVQIQYQRIRRNFMVACPLVLATRH----- 121
                   G   G  N    P+    ++    +I+ Q     +      + AT H     
Sbjct: 93  HGVFQLLSGWADGPENITQCPIRPGKKYTYRFKIKGQEGTLWWHAHSSWLRATVHGALLI 152

Query: 122 RPRRSSHPPPSPAYRCCTQSPTKRFQYCWVSGGMPTLSTLKRKASPPAAVLMAPTPTPLM 181
           RP+     P    Y+       + +    V      L+T  R  +   A  +   P  L 
Sbjct: 153 RPKFDLPLPYPTPYKKIPILLGEWWNANVVQVEQEGLAT-GRGPNGSDAYTINGHPGNLY 212

Query: 182 DSPETSTLAPKDLLA-------------------------SIVAVDATYTVPYVTDVIVL 241
              +  T   K +                           ++VAVDATYT PY+TDVIVL
Sbjct: 213 PCSQNQTYELKMVRGKTYLLQVINVALNNQLFFKLANHKFTVVAVDATYTDPYITDVIVL 272

Query: 242 APGQTTDVLVNADQPLGSYYMAARVWLMHCHLDVHLPWGLAMGFEVENGPTPST 262
           APGQTTDVLV A+QP+GSYYMAAR +       +  P  +     + +G +PST
Sbjct: 273 APGQTTDVLVKANQPIGSYYMAARPY-ADTQPQIGFPNSITRAVVIYDGASPST 316

BLAST of CmaCh03G003810.1 vs. ExPASy TrEMBL
Match: A0A6J1IKA9 (Laccase OS=Cucurbita maxima OX=3661 GN=LOC111478200 PE=3 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 6.2e-24
Identity = 117/317 (36.91%), Postives = 143/317 (45.11%), Query Frame = 0

Query: 2   TVEDMTVRRLSRDLVITAVNGEYPGPTIDVQDDDVLIVHLSNNSPYDLTIHCFLYHFQAW 61
           TV+DMTVRRL RD  ITAVNGEYPGPTI V D D+L+VH+SN SPYDLTIH        W
Sbjct: 32  TVQDMTVRRLCRDQEITAVNGEYPGPTIHVHDGDILVVHVSNKSPYDLTIH--------W 91

Query: 62  SIPVAKRVGRRSGKYN----PMSDTARWKLHVQIQYQRIRRNFMVACPLVLATRH----- 121
                   G   G  N    P+     +    +I+ Q     +      + AT H     
Sbjct: 92  HGVFQLLSGWADGPENVTQCPIRPGGNYTYRFKIKEQEGTLWWHAHSSWLRATVHGALLI 151

Query: 122 RPRRSSHPPPSPAYRCCTQSPTKRFQYC----W------------VSGGMPTLSTLKRKA 181
           RP +S  P P P        P K+        W             +G  P +S      
Sbjct: 152 RP-KSGRPLPYP-------KPYKKIPILLGEWWNANVVHVEEEGLATGSGPNISDAYTIN 211

Query: 182 SPPA-----------AVLMAPTPTPLMDSPETS-------TLAPKDLLASIVAVDATYTV 241
             P             + MA   T L+     +        LA  +L  ++VAVDA YT 
Sbjct: 212 GLPGNLYPCSQNQTYTLKMARGKTYLLQVINAALNNQFFFKLANHNL--TVVAVDAVYTD 271

Query: 242 PYVTDVIVLAPGQTTDVLVNADQPLGSYYMAARVWLMHCHLDVHLPWGLAMGFEVENGPT 272
           PYVTDVIVLAPGQTTDVLV ADQP  SYYMAAR +       +     +     V NG +
Sbjct: 272 PYVTDVIVLAPGQTTDVLVKADQPPRSYYMAARPY-ADARPPIDFSDTITRAIVVYNGAS 329

BLAST of CmaCh03G003810.1 vs. ExPASy TrEMBL
Match: A0A5A7TPK9 (Laccase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold78209G00430 PE=3 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 1.1e-23
Identity = 103/290 (35.52%), Postives = 136/290 (46.90%), Query Frame = 0

Query: 6   MTVRRLSRDLVITAVNGEYPGPTIDVQDDDVLIVHLSNNSPYDLTIHCFLYHFQAWSIPV 65
           M+VRRL RD VITAVNGEYPGPTI VQD+DVL+VH++NNSP+DLTIH        W    
Sbjct: 1   MSVRRLCRDQVITAVNGEYPGPTIHVQDEDVLVVHVTNNSPHDLTIH--------WHGVF 60

Query: 66  AKRVGRRSGKYN----PMSDTARWKLHVQIQYQRIRRNFMVACPLVLATRH-----RPRR 125
               G   G  N    P+    ++    +I+ Q     +      + AT H     RP+ 
Sbjct: 61  QLLSGWADGPENITQCPIRPGKKYTYRFKIKGQEGTLWWHAHSSWLRATVHGALLIRPKF 120

Query: 126 SSHPPPSPAYRCCTQSPTKRFQYCWVSGGMPTLSTLKRKASPPAAVLMAPTPTPLMDSPE 185
               P    Y+       + +    V      L+T  R  +   A  +   P  L    +
Sbjct: 121 DLPLPYPTPYKKIPILLGEWWNANVVQVEQEGLAT-GRGPNGSDAYTINGHPGNLYPCSQ 180

Query: 186 TSTLAPKDLLA-------------------------SIVAVDATYTVPYVTDVIVLAPGQ 245
             T   K +                           ++VAVDATYT PY+TDVIVLAPGQ
Sbjct: 181 NQTYELKMVRGKTYLLQVINVALNNQLFFKLANHKFTVVAVDATYTDPYITDVIVLAPGQ 240

Query: 246 TTDVLVNADQPLGSYYMAARVWLMHCHLDVHLPWGLAMGFEVENGPTPST 262
           TTDVLV A+QP+GSYYMAAR +       +  P  +     + +G +PST
Sbjct: 241 TTDVLVKANQPIGSYYMAARPY-ADTQPQIGFPNSITRAVVIYDGASPST 280

BLAST of CmaCh03G003810.1 vs. NCBI nr
Match: XP_022950373.1 (LOW QUALITY PROTEIN: laccase-7-like [Cucurbita moschata])

HSP 1 Score: 143.7 bits (361), Expect = 2.4e-30
Identity = 126/304 (41.45%), Postives = 150/304 (49.34%), Query Frame = 0

Query: 2   TVEDMTVRRLSRDLVITAVNGEYPGPTIDVQDDDVLIVHLSNNSPYDLTIHCFLYH--FQ 61
           TVEDMTVRRL RD VITAVNGEYPGPTI VQDDDVLIVH+SNNSPYDLTIH   +H  FQ
Sbjct: 29  TVEDMTVRRLCRDQVITAVNGEYPGPTIHVQDDDVLIVHVSNNSPYDLTIH---WHGVFQ 88

Query: 62  AWSIPVAKRVGRRSGKYNPMSDTARWKLHVQIQYQRIRRNFMVACPLVLATRH-----RP 121
             S   A   G  +    P+     +     I+ Q     +      + AT H     RP
Sbjct: 89  LLS---AWADGPENVTQCPIRPGGNYTYRFNIKGQEGTLWWHAHSSWLRATVHGALLIRP 148

Query: 122 RRSSHPPPSPAYRCCTQSPTKRFQYC---WVSGGMPTLS----TLKRKASPPAAVLMAPT 181
            +S  P P        Q P K+       W +  +  +        R  +   A  +   
Sbjct: 149 -KSGLPLP-------YQKPYKKVPILLGEWWNANVVHVEEEGLAAGRGPNGSDAYTINGL 208

Query: 182 PTPLMDSPETSTLAPK------DLL-------------------ASIVAVDATYTVPYVT 241
           P  L   P+  T   K       LL                    ++VAVDATYTVPYVT
Sbjct: 209 PGNLYPCPQNQTYQLKMARGKTSLLQVVNAALNNQLFFKLANHNLTVVAVDATYTVPYVT 268

Query: 242 DVIVLAPGQTTDVLVNADQPLGSYYMAARVWLMHCHLDVHLPWGLAMGFEVENG-PTPST 266
           DVIVLAPGQTTDVLVNADQPLGSYYMAAR +       +  P  +     + +G PT ST
Sbjct: 269 DVIVLAPGQTTDVLVNADQPLGSYYMAARPY-ADTRPQIAFPNTITRAIVIYDGAPTNST 317

BLAST of CmaCh03G003810.1 vs. NCBI nr
Match: XP_022950373.1 (LOW QUALITY PROTEIN: laccase-7-like [Cucurbita moschata])

HSP 1 Score: 99.4 bits (246), Expect = 5.2e-17
Identity = 41/42 (97.62%), Postives = 41/42 (97.62%), Query Frame = 0

Query: 232 VWLMHCHLDVHLPWGLAMGFEVENGPTPSTRLPPPPHDLPKC 274
           VWLMHCHLDVHLPWGLAMGFEV NGPTPSTRLPPPPHDLPKC
Sbjct: 527 VWLMHCHLDVHLPWGLAMGFEVANGPTPSTRLPPPPHDLPKC 568

HSP 2 Score: 138.7 bits (348), Expect = 7.7e-29
Identity = 107/329 (32.52%), Postives = 144/329 (43.77%), Query Frame = 0

Query: 3   VEDMTVRRLSRDLVITAVNGEYPGPTIDVQDDDVLIVHLSNNSPYDLTIHC--FLYHFQA 62
           VED+  + L R  VITAVNG  PGPTI+ ++ D +I+H+ N SPY+LTIH    +     
Sbjct: 1   VEDIDFQLLCRPQVITAVNGTLPGPTINAREGDTVIIHVFNKSPYNLTIHWHGIIQFLTP 60

Query: 63  WS--------IPVAKRVGRRSGKYN--PMSDTARWKLHVQIQYQRIRRNFMV-------- 122
           WS         P+    G  + ++N      T  W  H       +    ++        
Sbjct: 61  WSDGPEYVTQCPIPSN-GSYTYQFNLTGQEGTLWWHAHSSFLRATVYGALIIKPRLGHSY 120

Query: 123 -------ACPLVLA---------TRHRPRRSSHPPPSPAYRCCTQSPTKRFQYCWVSGGM 182
                    PLV+                 + +PP           P   F     S   
Sbjct: 121 PYPKVYQEVPLVVGEWWNANVEEVERNATDTGNPPLESIASTINGFPGDLFN---CSQDK 180

Query: 183 PTLSTLKRKASPPAAVLMAPTPTPLMDSPETSTLAPKDLLASIVAVDATYTVPYVTDVIV 242
             +  +KR  +    V+ A      ++      +A   L  ++VA+DA YT  Y TDVIV
Sbjct: 181 TYMLKVKRGKTYLLRVINA-----ALNEQHFFKVANHSL--TVVAMDAIYTEHYNTDVIV 240

Query: 243 LAPGQTTDVLVNADQPLGSYYMAAR----------------------VWLMHCHLDVHLP 274
           LAPGQT DVL+  +Q +GSYYM                         +W MHCHL+ HLP
Sbjct: 241 LAPGQTVDVLLRTNQAVGSYYMVFTPYRSSSVGTNNVTTRGVIVYDGIWFMHCHLETHLP 300

BLAST of CmaCh03G003810.1 vs. NCBI nr
Match: PPD80157.1 (hypothetical protein GOBAR_DD22901 [Gossypium barbadense])

HSP 1 Score: 135.6 bits (340), Expect = 6.5e-28
Identity = 114/317 (35.96%), Postives = 148/317 (46.69%), Query Frame = 0

Query: 3   VEDMTVRRLSRDLVITAVNGEYPGPTIDVQDDDVLIVHLSNNSPYDLTIHCFLYH--FQA 62
           V+++TV RL    +I AVNG +PGP I V++ D LIVH+ N SPY+LT+H   +H  FQ 
Sbjct: 31  VQNLTVTRLCNRQLIVAVNGSFPGPRIRVREGDTLIVHVYNKSPYNLTVH---WHGVFQK 90

Query: 63  WSIPVAKRVGRRSGKYNPMSDTARWKLHVQIQYQRIRRNFMVACPLVLATRH-----RPR 122
            S       G       PM    ++    +I  Q     +      + AT H     RP 
Sbjct: 91  LS---CWADGPNMITQCPMLPGNKYTYKFKISGQEGTLWWHAHVSWLRATVHGALIIRP- 150

Query: 123 RSSHPPPSPAYRCCTQSPTKRFQYCWVSGGM-------PTLSTLKRKASPPAAVLMAPTP 182
           +S H  P P      +  T      W +  M        +    K K  P    L+    
Sbjct: 151 KSGHKYPFPKPH---REVTIILGEWWNANVMDVEDEAVTSGDMYKLKVEPGKTYLLRIVN 210

Query: 183 TPLMDSPETSTLAPKDLLASIVAVDATYTVPYVTDVIVLAPGQTTDVLVNADQPLGSYYM 242
           T L +      +A  +L  ++VAVDA YT PYVT++I++APGQT D L+ ADQ +GSYYM
Sbjct: 211 TAL-NGQLFYKIANHNL--TVVAVDAAYTKPYVTNIIMIAPGQTVDALLVADQDIGSYYM 270

Query: 243 AAR--------------------------------VWLMHCHLDVHLPWGLAMGFEVENG 274
           AA                                 VW+MHCHLD HL +GL   F VENG
Sbjct: 271 AASPYSTSVGIPYDNTTTRGVVVYDGAPPIXDNPGVWIMHCHLDSHLTYGLGTVFMVENG 330

BLAST of CmaCh03G003810.1 vs. NCBI nr
Match: KAG7033800.1 (Laccase-7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 130.2 bits (326), Expect = 2.7e-26
Identity = 110/285 (38.60%), Postives = 142/285 (49.82%), Query Frame = 0

Query: 6   MTVRRLSRDLVITAVNGEYPGPTIDVQDDDVLIVHLSNNSPYDLTIHCFLYHFQAWSIPV 65
           MTVRRL RD VITAVNGEYPGPT+ VQDDDVLIVH+SNNSPYDLTIH   +H     +  
Sbjct: 1   MTVRRLCRDQVITAVNGEYPGPTVHVQDDDVLIVHVSNNSPYDLTIH---WHGVFQLLSA 60

Query: 66  AKRVGRRSGKYN--PMSDTARWKLH-------------------VQIQYQRIRRNFMVAC 125
               G  + ++N      T  W  H                   + + YQ+  +      
Sbjct: 61  WADGGNYTYRFNIKGQEGTLWWHAHSSWLRATVHGAILIRPKSGLPLPYQKPYKK----V 120

Query: 126 PLVLATRHRPRRSSHPPPSPAYRCCTQSPTKRFQYCWVSGGMPTLS---TLKRKASPPAA 185
           P++L          H            + +  +    + G +   S   T + K +    
Sbjct: 121 PILLGEWWNANVVVHVEEEGLAAGRGPNGSDAYTINGLPGNLYPCSQNQTYQLKMARGKT 180

Query: 186 VLMAPTPTPLMDSPETSTLAPKDLLASIVAVDATYTVPYVTDVIVLAPGQTTDVLVNADQ 245
            L+      L ++     LA  +L  ++VAVDATYTVPYVTDVIVLAPGQTTDVLVNADQ
Sbjct: 181 YLLQVINAAL-NNQLFFKLANHNL--TVVAVDATYTVPYVTDVIVLAPGQTTDVLVNADQ 240

Query: 246 PLGSYYMAARVWLMHCHLDVHLPWGLAMGFEVENG-PTPSTRLPP 266
            LGSYYMAAR +       +  P  +     + +G PT ST + P
Sbjct: 241 LLGSYYMAARPY-ADTRPQIAFPNTITRAIVIYDGAPTNSTPIMP 274

BLAST of CmaCh03G003810.1 vs. NCBI nr
Match: XP_038883561.1 (laccase-7-like [Benincasa hispida])

HSP 1 Score: 128.3 bits (321), Expect = 1.0e-25
Identity = 110/269 (40.89%), Postives = 134/269 (49.81%), Query Frame = 0

Query: 2   TVEDMTVRRLSRDLVITAVNGEYPGPTIDVQDDDVLIVHLSNNSPYDLTIHCFLYH--FQ 61
           TV+DMTVRRL R+  ITAVNGEYPGPTI VQD DVL+VH++NNSPYDLTIH   +H  FQ
Sbjct: 30  TVQDMTVRRLCREQEITAVNGEYPGPTIHVQDGDVLVVHVTNNSPYDLTIH---WHGVFQ 89

Query: 62  AWSIPVAKRVGRRSGKYNPMSDTARWKLHVQIQYQRIRRNFMVACPLVLATRH-----RP 121
             S   A   G  +    P+     +    QI+ Q     +      + AT H     RP
Sbjct: 90  LLS---AWADGPENITQCPIRPGGNYTYKFQIKEQEGTLWWHAHSSWLRATVHGALLIRP 149

Query: 122 RRSSHPPPSPAYRCCTQSPTKRFQYC----W------------VSGGMPTLSTLKRKASP 181
            +S+ P P P        P K+        W             +G  P +S        
Sbjct: 150 -KSNRPLPYP-------KPYKKIPILLGEWWNANVVHVEEEGLATGSGPNISDAYTINGL 209

Query: 182 PAAVL-MAPTPTPLMDSPETSTLAPKDLLA---------------SIVAVDATYTVPYVT 232
           P  +   +   T  +      T   + + A               ++VAVDATYT PYVT
Sbjct: 210 PGNLYPCSQNQTYQLKMVRGKTYLLQVINAALNNQFFFKFANHNFTVVAVDATYTDPYVT 269

BLAST of CmaCh03G003810.1 vs. TAIR 10
Match: AT5G01040.1 (laccase 8 )

HSP 1 Score: 80.5 bits (197), Expect = 2.3e-15
Identity = 83/304 (27.30%), Postives = 133/304 (43.75%), Query Frame = 0

Query: 3   VEDMTVRRLSRDLVITAVNGEYPGPTIDVQDDDVLIVHLSNNSPYDLTIHCFLYH--FQA 62
           ++D+ V+ L ++ +I A NG  PGPTI+V++ D L+V++ NNS Y++TIH   +H  FQ 
Sbjct: 34  IQDVVVKPLCKEQIIPAANGSLPGPTINVREGDTLVVNVINNSTYNVTIH---WHGVFQL 93

Query: 63  WSIPVAKRVGRRSGKYNPMSDTARWKLHVQIQYQRIRRNFMVACPLVLATRH-----RPR 122
            S+ +    G       P+     +     I  Q     +      + AT H     RP 
Sbjct: 94  KSVWMD---GANMITQCPIQPGYNFTYQFDITGQEGTLLWHAHVVNLRATLHGALVIRP- 153

Query: 123 RSSHPPPSPAYRCCTQSPTKRFQYCWVSGGMPTLSTLKRKASPPAAVL----MAPTPTPL 182
           RS  P P P        P K     +       +  L+ + +P +       +A    P 
Sbjct: 154 RSGRPYPFP-------KPYKEVPIVFQQWWDTDVRLLQLRPAPVSDAYLINGLAGDSYPC 213

Query: 183 MDSP--ETSTLAPKDLL--------------------ASIVAVDATYTVPYVTDVIVLAP 242
            ++       +  K  L                     ++VAVDA Y+ PY+TDV++L P
Sbjct: 214 SENRMFNLKVVQGKTYLLRIVNAALNTHLFFKIANHNVTVVAVDAVYSTPYLTDVMILTP 273

Query: 243 GQTTDVLVNADQPLGSYYMAARVWLMHCHL---DVHLPWGLAMGFEVENGPTPSTRLPPP 271
           GQT D L+ ADQ +G YYMA   ++    +   D+    GL +     +  +P+  L P 
Sbjct: 274 GQTVDALLTADQAIGKYYMATLPYISAIGIPTPDIKPTRGLIVYQGATSSSSPAEPLMPV 323

BLAST of CmaCh03G003810.1 vs. TAIR 10
Match: AT5G01050.1 (Laccase/Diphenol oxidase family protein )

HSP 1 Score: 79.0 bits (193), Expect = 6.8e-15
Identity = 85/306 (27.78%), Postives = 127/306 (41.50%), Query Frame = 0

Query: 3   VEDMTVRRLSRDLVITAVNGEYPGPTIDVQDDDVLIVHLSNNSPYDLTIHCFLYH--FQA 62
           V+D+ V  L ++ +I  VNG  PGPTI+V++ D L+VH+ N S Y++TIH   +H  FQ 
Sbjct: 34  VKDVVVTPLCKEQMIPIVNGSLPGPTINVREGDTLVVHVINKSTYNVTIH---WHGVFQL 93

Query: 63  WSIPVAKRVGRRSGKYNPMSDTARWKLHVQIQYQRIRRNFMVACPLVLATRH-----RPR 122
            S+ +    G       P+  +  +     I  Q     +      + AT H     RP 
Sbjct: 94  KSVWMD---GANMITQCPIQPSNNFTYQFDITGQEGTLLWHAHVVNLRATIHGALIIRP- 153

Query: 123 RSSHPPPSPAYRCCTQSPTKRFQYCWVSGGMPTLSTLKRKASPPAAVLMA------PTPT 182
           RS  P P P        P K     +       +  L+ + +P +   +         P 
Sbjct: 154 RSGRPYPFP-------KPYKEVPLIFQQWWDTDVRLLELRPAPVSDAYLINGLAGDSYPC 213

Query: 183 PLMDSPETSTLAPKDLL--------------------ASIVAVDATYTVPYVTDVIVLAP 242
                     +  K  L                     ++VAVDA YT PY+TDV++L P
Sbjct: 214 SKNRMFNLKVVQGKTYLLRIINAALNTHLFFKIANHNVTVVAVDAVYTTPYLTDVMILTP 273

Query: 243 GQTTDVLVNADQPLGSYYMAARVWLMHCHLDVHLPWGLAMGFEVENGPT----PSTRLPP 272
           GQT D ++ ADQP+G+YYMA   +     +          G  V  G T    P+    P
Sbjct: 274 GQTIDAILTADQPIGTYYMAIIPYFSAIGVPASPDTKPTRGLIVYEGATSSSSPTKPWMP 325

BLAST of CmaCh03G003810.1 vs. TAIR 10
Match: AT3G09220.1 (laccase 7 )

HSP 1 Score: 73.9 bits (180), Expect = 2.2e-13
Identity = 30/41 (73.17%), Postives = 34/41 (82.93%), Query Frame = 0

Query: 233 WLMHCHLDVHLPWGLAMGFEVENGPTPSTRLPPPPHDLPKC 274
           W+ HCH+DVHLP+GL M F V+NGPT ST LPPPP DLPKC
Sbjct: 527 WIFHCHIDVHLPFGLGMIFVVKNGPTKSTTLPPPPPDLPKC 567

HSP 2 Score: 73.2 bits (178), Expect = 3.7e-13
Identity = 78/268 (29.10%), Postives = 108/268 (40.30%), Query Frame = 0

Query: 3   VEDMTVRRLSRDLVITAVNGEYPGPTIDVQDDDVLIVHLSNNSPYDLTIHC--FLYHFQA 62
           V+++TV RL +  VIT VNG  PGPTI V++ D L++H+ N+SP+++TIH     +    
Sbjct: 32  VQNLTVSRLCKRQVITVVNGSLPGPTIRVKEGDSLVIHVLNHSPHNITIHWHGIFHKLTV 91

Query: 63  WSIPVAKRVGRRSGKYNPMSDTARWKLHVQIQYQRIRRNFMVACPLVLATRH-----RPR 122
           W+       G       P+    R+     I  Q     +      + AT +     RP 
Sbjct: 92  WA------DGPSMITQCPIQPGQRYAYRFNITGQEGTLWWHAHASFLRATVYGALVIRP- 151

Query: 123 RSSHPPPSPAYRCCTQSPTKRFQYCWVSGGMPTLSTLKRKASPPAAVLMAPTPTPLMDSP 182
           +S H  P P        P K     +       +  L+  A              +   P
Sbjct: 152 KSGHSYPFP-------KPHKEVPILFGEWWNTDVVALEEAAIATGVPPNNSDAYTINGRP 211

Query: 183 ETSTLAPKDLLAS--------------------------------IVAVDATYTVPYVTD 231
                  KD + S                                +VA DA YT PYVTD
Sbjct: 212 GNLYPCSKDRMFSLNVVKGKRYLLRIINAAMNIQLFFKIANHRLTVVAADAVYTAPYVTD 271

BLAST of CmaCh03G003810.1 vs. TAIR 10
Match: AT1G18140.1 (laccase 1 )

HSP 1 Score: 72.8 bits (177), Expect = 4.8e-13
Identity = 34/75 (45.33%), Postives = 43/75 (57.33%), Query Frame = 0

Query: 199 PYVTDVIVLAPGQTTDVLVNADQPLGSYYMAARVWLMHCHLDVHLPWGLAMGFEVENGPT 258
           P   +   +  G    + +NAD P         VW +HCHL+ H  WGLAMGF V++GP 
Sbjct: 515 PPERNTFAVPTGGWAAIRINADNP--------GVWFIHCHLEQHTSWGLAMGFIVKDGPL 574

Query: 259 PSTRLPPPPHDLPKC 274
           PS  L PPPHDLP+C
Sbjct: 575 PSQTLLPPPHDLPQC 581

HSP 2 Score: 43.1 bits (100), Expect = 4.1e-04
Identity = 19/50 (38.00%), Postives = 30/50 (60.00%), Query Frame = 0

Query: 3  VEDMTVRRLSRDLVITAVNGEYPGPTIDVQDDDVLIVHLSNNSPYDLTIH 53
          VE   V RL     +  VNG+YPGPT+ V + D++ + ++N   ++ TIH
Sbjct: 35 VEWKKVTRLCHTKQLLTVNGQYPGPTVAVHEGDIVEIKVTNRIAHNTTIH 84

BLAST of CmaCh03G003810.1 vs. TAIR 10
Match: AT5G05390.1 (laccase 12 )

HSP 1 Score: 67.8 bits (164), Expect = 1.6e-11
Identity = 70/264 (26.52%), Postives = 106/264 (40.15%), Query Frame = 0

Query: 3   VEDMTVRRLSRDLVITAVNGEYPGPTIDVQDDDVLIVHLSNNSPYDLTIHCFLYHFQAWS 62
           +++  V+RL +      VNG +PGPT++V + D L V + N + Y++TIH        W 
Sbjct: 33  IQETPVKRLCKTRNAITVNGMFPGPTLEVNNGDTLEVKVHNRARYNITIH--------WH 92

Query: 63  IPVAKRVGRRSG----KYNPMSDTARWKLHVQIQYQ------RIRRNFMVACPLVLATRH 122
                R G   G       P+     +     IQ Q          +++ A        H
Sbjct: 93  GVRQIRTGWADGPEFVTQCPIRPGKSYTYRFTIQGQEGTLWWHAHSSWLRATVYGALIIH 152

Query: 123 RPRRSSHPPPSPAYRCC-------TQSPTKRFQYCWVSGGMPTLSTLKRKASPPAAVLMA 182
               SS P P P  +           +P         +G  P +S        P  +   
Sbjct: 153 PTPGSSFPFPKPDRQTALMLGEWWNANPVDVINQATRTGAAPNISDAYTINGQPGDLYNC 212

Query: 183 PTPTPL---MDSPETSTL-------------APKDLLASIVAVDATYTVPYVTDVIVLAP 234
            T   +   ++S ETS L                +   ++V  DA+Y  P+ T V++L P
Sbjct: 213 STKETVVVPINSGETSLLRVINAALNQPLFFTVANHKLTVVGADASYLKPFTTKVLMLGP 272

HSP 2 Score: 65.9 bits (159), Expect = 5.9e-11
Identity = 28/42 (66.67%), Postives = 30/42 (71.43%), Query Frame = 0

Query: 232 VWLMHCHLDVHLPWGLAMGFEVENGPTPSTRLPPPPHDLPKC 274
           VWLMHCHLDVH+ WGLAM F V+NG      L  PPHDLP C
Sbjct: 524 VWLMHCHLDVHIKWGLAMAFLVDNGVGELETLEAPPHDLPIC 565