CcUC07G135880.1 (mRNA) Watermelon (PI 537277) v1

Overview
NameCcUC07G135880.1
TypemRNA
OrganismCitrullus colocynthis (Watermelon (PI 537277) v1)
DescriptionSubtilisin-like protease SBT3.4
LocationCicolChr07: 19012101 .. 19016749 (+)
Sequence length2289
RNA-Seq ExpressionCcUC07G135880.1
SyntenyCcUC07G135880.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTCAATTTTCCGCAGCTTATATGTTTACTCAATTTGATCAGCTTCTTCATATCGTACACCTCGAACATAACCAACATGATCCCAACTCCAGCATCGTCGAGATTCATCATAATTTCTTGTCTACTCTGCTGGGAAGGTAATCTAAATTCTAAATTTCAAAACTCTTCCGAGATTGAATTCAACTAAAACTCTGATCATCTTATTGAAGTAAAAAGAAGGCTTCGAAATCCATGGTGTACAGTTATACACATGCCTTTTCTGGGTTTGCTGCAAAGCTCACAAAATCTCAAGCTTACCAGCTTTCTGGTATTTGAACATACAATTTTTTCTGTATTTCCGACGTGGGTTTTGTTAATAACTCGGTTCTTTGATTTTATGAAATGAACTCTTAGAAATGCGTGGTGTGGTTCGAGTTCTTCCAAGTGTTCTTTACGATCTCCATACAACTAGAAGCTGGGATTTTCTCGGCCTTTCTCCTTCTCCATCTCCCTCAAATCTTCTGCAGATGAGTAATTTGGGTGACGGAATAATCATTGGAGTAGTGGATACAGGTCAGTTTAAGTTTTTCGGTTTAGTGGTGATTTAATATGGTATTCCGATAAGTAATAACCATTTCCGATACAGGAATTTGGCCGGAAACGGAGGCGTTCGACGATCAAGGATTGGGGCCGGTGCCAGTTCGGTGGAAAGGCGAGTGCCAATCAGGGGAACAATTCAAAGCGACGAGAGATTGCAATAGAAAAATTATAGGAGCCAAATGGTACGTTGATGGGTTCATAGCCGAATTTGGGGAATTCAACAGATCAGCAGTGAAGGAATTCATGTCTCCAAGAGATTCAAATGGACATGGGACTCAAACGGCGAGCACCGCCGCCGGTTCGTTCGTGAAAAATGTCAGTTACAGAGGCCTCGGCGGTGGGACTGCAAGGGGCGGCGCACCAGCAGCGCGGTTGGCAGTGTACAAGGCATGTTGGAGCTTGCCCGGTGGCGGTGGCCAGTGTTCGGCGGCGGACGTATTGAAGGGAATGGACGACGCCGTACACGACGGCGTTGATGTATTGTCTCTATCTTTGGGGAAGAGTGCTCCTCTGTTTGCGGAGGTGGATGAAGAGAACGTGGTTGCGATTGGGTCATTCCGGGCAAACATGAGGAATATTTCTGTGGTCTGTTCTGCCGGTAACGACGGGCCTTCTGCAGAGACGGTGATCAACACTTCCCCTTGGATCTTGAGTGTTGCTGCAACTTCTATTGACAGAACTTTTTTGGGCTCTATTACTCTCGGAAATAACAACACTTTTCTGATAAGAGACAGACGCAAATGCTTCTAATGAAATTACTTTTACCAAATCGTTCTAAACAAATTGTAAATCATAAATTCTCATCTTAATCTTATTTTTGTGGCAGAGTCAAACCCTATACACTAGAAAGGAGATTGGATATAGTGGCTTGGTATATCCAAGGTCTGGGTTGAACCAGATCATCGCAGGGTTAGTTAACCTAAAAAACCATTTTCCCCCATCATCTATTAATACCCATATGAATATGAATATGAATACTAACTACAAGATGGATAACTTATAAAGTATTAACATTGATATTTGAACGTGAGTGAATGACCCAATTTGTAGGTTATGTGATTCAATAACATTAGTGAATGGTAGTAATTGGATGAGTGGAAAAGTGGTTCTCTGCTTCAGCACAATGGTGGGGATGAATCCAATAGCCATAACAAGAGCTGTGGAAGCTGTGAAAGTAGGAAATGGCTTGGGGTTAATTGTTGCTAGGCATCCTGATGATATCTGGTTTACATGTGACAATGATTTCCCTTGTTTTCAAGTCAATTTTGAAGTTGGCACCAAAATCTTCTATTACATCCGGGCAACAAGGTAATGTCCTCGAAACTTAAATTGTATACACATAATATGTTATTTATGATTTCAAGTTGATCCCATTGTGCCTTTGCATCTATATCATTTGATATTTCTCTATAATAATAAAATTGCTCTCTTCCTTTGTCCGTAGACGTATATAACACATTGTTAGTAAATGACGTAAATTTGTATATCAATTACTCTACGTTCTTTATTCTTTACTTGTTAGTTTTGTTTCTGTCTTAACATCCCTCTTTAATTTTTCGTTACTTTGATTTTGCTTAATGAAATGTATAGATCTCCAATGGTAAAGCTGAGCCCTTCAAGGACCATCACAGGCAACCCCATTTCAGCACATATAGCATACTTCTCATCAAGAGGTCCCAATTCTGTTGCACCAGCAATTCTGAAGGTAACTTAAATAAGTATTTGTACATATATATTATGGTTTGTTGATGTTATTTTTTCTGAATCTAAGTATTGAACAGCCAGATATTGGTGCTCCAGGTGTGGCAATATTAGTAGCCACTTCCCCATTTGACATAACAAATGATGGAGGTTTTACTATACAAACAGGAACTTCCATAGCAACCCCTCACATTTCAGCCATTGTGGCTCTTCTCAAGTCACTTCATCCTACTTGGTCTCCTGCTGCCATCAAATCTGCCATTATCACAACTGGTATTCCCAATTTCTCCTTACATTTTTCTTTTCCTCAACTCGATAATCATTTTGGACTTTTGAAAATTAAATTTGGACGTCTCTCACCATTTCTTTACGGTAGTTTTTGTTTTTCTCAAGAACATTTGAATTTTTAGACAAATTTTAAGACCAACAAGAAGGTTTTAGAAACTAATCTTTTTTCCTTAGTTTTCAAGACTTTGCCTTGAATTTTATTTTCTTTTATTTATTTATTATTATTTTTTTTTTTATAAAATCTAAATAACTAATCAAAGAATTTCTTGGGTGGAAATAGTATTTTTAAGCTATTTTTAAGTTTTAAAAATGATGGCTTGTTTGATGAAGAATCTATTTTATTTTTTTTTCTATTTTTTAAATTTAAGCATGTTTCCCCCAAATTTTTAATTATGATTTTTAAATTTGTTAACTAAACACACTAGAATATCTAGTAAAATAAAAAAATATTGAAACTATCCATATATAGTTTTCAAAATTTGACTTAGTTTTTGAAACCTTAGTAGAAAATAGATAATAAGATATAGGAAAATAGCAAGTTGAAGCAATATTTATAAGCGGCATTTTTAAAAGTTGAAAACAAAGAAGTAAATGGTTATCAAAAGAGGCTCAAATGGATATCAAAGGAGAACATAGGACCTATTTGGTAGGCAATTTGAAAGCAAGGGATTCAAAGAAAACAAAATTTGTATTTCATGTTTTGAGGTTTGTGTTTAATAAGAGATTAAAAAATTAAATTCCAATATAAACAATTTTTCAAAATGTGTTTGATAATATATTTATAAACCAAATATTATGTTTAATATAAACTATAATTCTTGTTTTACGTCAATTTTTTTTATAACAATTATTTTATAATATCATATAATATTTAATCTATTATACAACACATATTTTAATTTTACAAAAATAATTGAGTTTATAACATGAAATACTATATATATATATTTTTATTCTTTTTATCTTTTCTAATATAATTCTTCATTCTTAAATTTTAGATTCAAAATTTGGATACACAGAAAACATAAAAATCTTGTTTGTGAAATTTCCACGAAAGAGAATTCGAAAACAATTTTTGAAGACATAGTCCACACTATCTACCAAACACATATTCGCTTAATTCAGTGAATCAGGAGATAGAAAACATAAATCAGAATTTGAATTACCTACCAAACATGTCTTTAGTTTACAATATGTAGTTTGAACTTTTAAAATCATGTAGATAACAAAATAAAGATACTCACAGATGAAAGTATAATGTCGATAATTGTCTAAAAAAAAGTAATGTTTATAAGCTTAATTTTCATAAATCAAAAACCAAAAATAAAACCAGGCTTAGTTTTAAAAAAACCAATTTTTTTTTATTGAAATTTGACTAATAATTTAAATGCTATTTTAAGAAAGACGAACAGCATTATACAAAAAGTTAAAGAGAAAATAAGCACAATTTAAAAAAAAAATTCAACTGATTAAGATTTAAAGCTAAAATAAATGAATTATTTTGAGTTGCAGCATGGAGCAGGCATCCTTCTGGGGTACCAATTCTGGCTGAAGGATCCCCACATAAACTTGGCAATCCATTTGACTATGGAGGAGGAATTGTTAACCCAAATGCAGCTGCAAACCCAGGCCTCATTTACGACATGTCGACTTCCGATTACATCTCTTACTTTTGCTCAATGGGCTACAAAAACTCTGCCATATCTTTTCTAACAGAGCAAAAAACAGAGTGCCCATCAAAACCCCAAATTTCTTTGTTGGATCTAAATCTACCTTCCATAACAGTCCCAGCACTTACAAACTCAACCACTATCACTCGAACTGTCACTAATGTTGGTAATTTGACCTCCATTTACAAAGCTTTGATTCACCCTCCAATGGGAATCAATGTCACTGTGGACCCTCTTGTCTTACTCTTCAATGCTACTGTCACCAATTTGTCCTTCAAGGTTATGATTTCAACCCTTTTAGAGATGGATTATGGTTTTTCTTTCGGAGCTATCATTTGGAGTGATGGAGTGCATCTTGTTACAACTCCATTGTCAGTTAGAACTCAAATTCTACATTCTTCTACGTAG

mRNA sequence

ATGATTCAATTTTCCGCAGCTTATATGTTTACTCAATTTGATCAGCTTCTTCATATCGTACACCTCGAACATAACCAACATGATCCCAACTCCAGCATCGTCGAGATTCATCATAATTTCTTGTCTACTCTGCTGGGAAGTAAAAAGAAGGCTTCGAAATCCATGGTGTACAGTTATACACATGCCTTTTCTGGGTTTGCTGCAAAGCTCACAAAATCTCAAGCTTACCAGCTTTCTGAAATGCGTGGTGTGGTTCGAGTTCTTCCAAGTGTTCTTTACGATCTCCATACAACTAGAAGCTGGGATTTTCTCGGCCTTTCTCCTTCTCCATCTCCCTCAAATCTTCTGCAGATGAGTAATTTGGGTGACGGAATAATCATTGGAGTAGTGGATACAGGAATTTGGCCGGAAACGGAGGCGTTCGACGATCAAGGATTGGGGCCGGTGCCAGTTCGGTGGAAAGGCGAGTGCCAATCAGGGGAACAATTCAAAGCGACGAGAGATTGCAATAGAAAAATTATAGGAGCCAAATGGTACGTTGATGGGTTCATAGCCGAATTTGGGGAATTCAACAGATCAGCAGTGAAGGAATTCATGTCTCCAAGAGATTCAAATGGACATGGGACTCAAACGGCGAGCACCGCCGCCGGTTCGTTCGTGAAAAATGTCAGTTACAGAGGCCTCGGCGGTGGGACTGCAAGGGGCGGCGCACCAGCAGCGCGGTTGGCAGTGTACAAGGCATGTTGGAGCTTGCCCGGTGGCGGTGGCCAGTGTTCGGCGGCGGACGTATTGAAGGGAATGGACGACGCCGTACACGACGGCGTTGATGTATTGTCTCTATCTTTGGGGAAGAGTGCTCCTCTGTTTGCGGAGGTGGATGAAGAGAACGTGGTTGCGATTGGGTCATTCCGGGCAAACATGAGGAATATTTCTGTGGTCTGTTCTGCCGGTAACGACGGGCCTTCTGCAGAGACGGTGATCAACACTTCCCCTTGGATCTTGAGTGTTGCTGCAACTTCTATTGACAGAACTTTTTTGGGCTCTATTACTCTCGGAAATAACAACACTTTTCTGAGTCAAACCCTATACACTAGAAAGGAGATTGGATATAGTGGCTTGGTATATCCAAGGTCTGGGTTGAACCAGATCATCGCAGGGTTATGTGATTCAATAACATTAGTGAATGGTAGTAATTGGATGAGTGGAAAAGTGGTTCTCTGCTTCAGCACAATGGTGGGGATGAATCCAATAGCCATAACAAGAGCTGTGGAAGCTGTGAAAGTAGGAAATGGCTTGGGGTTAATTGTTGCTAGGCATCCTGATGATATCTGGTTTACATGTGACAATGATTTCCCTTGTTTTCAAGTCAATTTTGAAGTTGGCACCAAAATCTTCTATTACATCCGGGCAACAAGATCTCCAATGGTAAAGCTGAGCCCTTCAAGGACCATCACAGGCAACCCCATTTCAGCACATATAGCATACTTCTCATCAAGAGGTCCCAATTCTGTTGCACCAGCAATTCTGAAGCCAGATATTGGTGCTCCAGGTGTGGCAATATTAGTAGCCACTTCCCCATTTGACATAACAAATGATGGAGGTTTTACTATACAAACAGGAACTTCCATAGCAACCCCTCACATTTCAGCCATTGTGGCTCTTCTCAAGTCACTTCATCCTACTTGGTCTCCTGCTGCCATCAAATCTGCCATTATCACAACTGCATGGAGCAGGCATCCTTCTGGGGTACCAATTCTGGCTGAAGGATCCCCACATAAACTTGGCAATCCATTTGACTATGGAGGAGGAATTGTTAACCCAAATGCAGCTGCAAACCCAGGCCTCATTTACGACATGTCGACTTCCGATTACATCTCTTACTTTTGCTCAATGGGCTACAAAAACTCTGCCATATCTTTTCTAACAGAGCAAAAAACAGAGTGCCCATCAAAACCCCAAATTTCTTTGTTGGATCTAAATCTACCTTCCATAACAGTCCCAGCACTTACAAACTCAACCACTATCACTCGAACTGTCACTAATGTTGGTAATTTGACCTCCATTTACAAAGCTTTGATTCACCCTCCAATGGGAATCAATGTCACTGTGGACCCTCTTGTCTTACTCTTCAATGCTACTGTCACCAATTTGTCCTTCAAGGTTATGATTTCAACCCTTTTAGAGATGGATTATGGTTTTTCTTTCGGAGCTATCATTTGGAGTGATGGAGTGCATCTTGTTACAACTCCATTGTCAGTTAGAACTCAAATTCTACATTCTTCTACGTAG

Coding sequence (CDS)

ATGATTCAATTTTCCGCAGCTTATATGTTTACTCAATTTGATCAGCTTCTTCATATCGTACACCTCGAACATAACCAACATGATCCCAACTCCAGCATCGTCGAGATTCATCATAATTTCTTGTCTACTCTGCTGGGAAGTAAAAAGAAGGCTTCGAAATCCATGGTGTACAGTTATACACATGCCTTTTCTGGGTTTGCTGCAAAGCTCACAAAATCTCAAGCTTACCAGCTTTCTGAAATGCGTGGTGTGGTTCGAGTTCTTCCAAGTGTTCTTTACGATCTCCATACAACTAGAAGCTGGGATTTTCTCGGCCTTTCTCCTTCTCCATCTCCCTCAAATCTTCTGCAGATGAGTAATTTGGGTGACGGAATAATCATTGGAGTAGTGGATACAGGAATTTGGCCGGAAACGGAGGCGTTCGACGATCAAGGATTGGGGCCGGTGCCAGTTCGGTGGAAAGGCGAGTGCCAATCAGGGGAACAATTCAAAGCGACGAGAGATTGCAATAGAAAAATTATAGGAGCCAAATGGTACGTTGATGGGTTCATAGCCGAATTTGGGGAATTCAACAGATCAGCAGTGAAGGAATTCATGTCTCCAAGAGATTCAAATGGACATGGGACTCAAACGGCGAGCACCGCCGCCGGTTCGTTCGTGAAAAATGTCAGTTACAGAGGCCTCGGCGGTGGGACTGCAAGGGGCGGCGCACCAGCAGCGCGGTTGGCAGTGTACAAGGCATGTTGGAGCTTGCCCGGTGGCGGTGGCCAGTGTTCGGCGGCGGACGTATTGAAGGGAATGGACGACGCCGTACACGACGGCGTTGATGTATTGTCTCTATCTTTGGGGAAGAGTGCTCCTCTGTTTGCGGAGGTGGATGAAGAGAACGTGGTTGCGATTGGGTCATTCCGGGCAAACATGAGGAATATTTCTGTGGTCTGTTCTGCCGGTAACGACGGGCCTTCTGCAGAGACGGTGATCAACACTTCCCCTTGGATCTTGAGTGTTGCTGCAACTTCTATTGACAGAACTTTTTTGGGCTCTATTACTCTCGGAAATAACAACACTTTTCTGAGTCAAACCCTATACACTAGAAAGGAGATTGGATATAGTGGCTTGGTATATCCAAGGTCTGGGTTGAACCAGATCATCGCAGGGTTATGTGATTCAATAACATTAGTGAATGGTAGTAATTGGATGAGTGGAAAAGTGGTTCTCTGCTTCAGCACAATGGTGGGGATGAATCCAATAGCCATAACAAGAGCTGTGGAAGCTGTGAAAGTAGGAAATGGCTTGGGGTTAATTGTTGCTAGGCATCCTGATGATATCTGGTTTACATGTGACAATGATTTCCCTTGTTTTCAAGTCAATTTTGAAGTTGGCACCAAAATCTTCTATTACATCCGGGCAACAAGATCTCCAATGGTAAAGCTGAGCCCTTCAAGGACCATCACAGGCAACCCCATTTCAGCACATATAGCATACTTCTCATCAAGAGGTCCCAATTCTGTTGCACCAGCAATTCTGAAGCCAGATATTGGTGCTCCAGGTGTGGCAATATTAGTAGCCACTTCCCCATTTGACATAACAAATGATGGAGGTTTTACTATACAAACAGGAACTTCCATAGCAACCCCTCACATTTCAGCCATTGTGGCTCTTCTCAAGTCACTTCATCCTACTTGGTCTCCTGCTGCCATCAAATCTGCCATTATCACAACTGCATGGAGCAGGCATCCTTCTGGGGTACCAATTCTGGCTGAAGGATCCCCACATAAACTTGGCAATCCATTTGACTATGGAGGAGGAATTGTTAACCCAAATGCAGCTGCAAACCCAGGCCTCATTTACGACATGTCGACTTCCGATTACATCTCTTACTTTTGCTCAATGGGCTACAAAAACTCTGCCATATCTTTTCTAACAGAGCAAAAAACAGAGTGCCCATCAAAACCCCAAATTTCTTTGTTGGATCTAAATCTACCTTCCATAACAGTCCCAGCACTTACAAACTCAACCACTATCACTCGAACTGTCACTAATGTTGGTAATTTGACCTCCATTTACAAAGCTTTGATTCACCCTCCAATGGGAATCAATGTCACTGTGGACCCTCTTGTCTTACTCTTCAATGCTACTGTCACCAATTTGTCCTTCAAGGTTATGATTTCAACCCTTTTAGAGATGGATTATGGTTTTTCTTTCGGAGCTATCATTTGGAGTGATGGAGTGCATCTTGTTACAACTCCATTGTCAGTTAGAACTCAAATTCTACATTCTTCTACGTAG

Protein sequence

MIQFSAAYMFTQFDQLLHIVHLEHNQHDPNSSIVEIHHNFLSTLLGSKKKASKSMVYSYTHAFSGFAAKLTKSQAYQLSEMRGVVRVLPSVLYDLHTTRSWDFLGLSPSPSPSNLLQMSNLGDGIIIGVVDTGIWPETEAFDDQGLGPVPVRWKGECQSGEQFKATRDCNRKIIGAKWYVDGFIAEFGEFNRSAVKEFMSPRDSNGHGTQTASTAAGSFVKNVSYRGLGGGTARGGAPAARLAVYKACWSLPGGGGQCSAADVLKGMDDAVHDGVDVLSLSLGKSAPLFAEVDEENVVAIGSFRANMRNISVVCSAGNDGPSAETVINTSPWILSVAATSIDRTFLGSITLGNNNTFLSQTLYTRKEIGYSGLVYPRSGLNQIIAGLCDSITLVNGSNWMSGKVVLCFSTMVGMNPIAITRAVEAVKVGNGLGLIVARHPDDIWFTCDNDFPCFQVNFEVGTKIFYYIRATRSPMVKLSPSRTITGNPISAHIAYFSSRGPNSVAPAILKPDIGAPGVAILVATSPFDITNDGGFTIQTGTSIATPHISAIVALLKSLHPTWSPAAIKSAIITTAWSRHPSGVPILAEGSPHKLGNPFDYGGGIVNPNAAANPGLIYDMSTSDYISYFCSMGYKNSAISFLTEQKTECPSKPQISLLDLNLPSITVPALTNSTTITRTVTNVGNLTSIYKALIHPPMGINVTVDPLVLLFNATVTNLSFKVMISTLLEMDYGFSFGAIIWSDGVHLVTTPLSVRTQILHSST
Homology
BLAST of CcUC07G135880.1 vs. NCBI nr
Match: XP_022136960.1 (subtilisin-like protease SBT3.5 isoform X2 [Momordica charantia])

HSP 1 Score: 1157.9 bits (2994), Expect = 0.0e+00
Identity = 578/744 (77.69%), Postives = 649/744 (87.23%), Query Frame = 0

Query: 15  QLLHIVHLEHNQHDPNSSIVEIHHNFLSTLLGSKKKASKSMVYSYTHAFSGFAAKLTKSQ 74
           +LLHIV+L   +          HH+ L+T+LGSKKKASK+ VYSY HAFSGFAAKLTKSQ
Sbjct: 31  ELLHIVYLGEKKQ---------HHDLLATILGSKKKASKATVYSYKHAFSGFAAKLTKSQ 90

Query: 75  AYQLSEMRGVVRVLPSVLYDLHTTRSWDFLGLSPSPSPSNLLQMSNLGDGIIIGVVDTGI 134
           A+QL+E+ GV+RVLP+VLYDLHTTRSWDFLG+SPSP   NLL  SNLGDGIIIG++D+GI
Sbjct: 91  AHQLAEIPGVLRVLPNVLYDLHTTRSWDFLGVSPSPYSPNLLHSSNLGDGIIIGIIDSGI 150

Query: 135 WPETEAFDDQGLGPVPVRWKGECQSGEQFKATRDCNRKIIGAKWYVDGFIAEFGEFNRSA 194
           WPE+EAF+D GLGPVP RW+G C+SGE+F A+R+CNRKIIGA+WYVDGF AEFGEFNRS+
Sbjct: 151 WPESEAFNDDGLGPVPRRWRGTCKSGEKFNASRNCNRKIIGARWYVDGFKAEFGEFNRSS 210

Query: 195 VKEFMSPRDSNGHGTQTASTAAGSFVKNVSYRGLGGGTARGGAPAARLAVYKACWSLPGG 254
             EF+SPRDSNGHGTQTASTAAGSFV NVSYRGL GGT RGGAP ARLA+YKACWSLP G
Sbjct: 211 ATEFISPRDSNGHGTQTASTAAGSFVSNVSYRGLAGGTLRGGAPRARLAMYKACWSLPSG 270

Query: 255 GGQCSAADVLKGMDDAVHDGVDVLSLSLGKSAPLFAEVDEENVVAIGSFRANMRNISVVC 314
           GGQC+AADVLK +DDAV D VDVLSLSLGKS PLF EVDEENVVAIGSF A  RNI VVC
Sbjct: 271 GGQCAAADVLKALDDAVQDRVDVLSLSLGKSVPLFPEVDEENVVAIGSFHAISRNIPVVC 330

Query: 315 SAGNDGPSAETVINTSPWILSVAATSIDRTFLGSITLGNNNTFLSQTLYTRKEIGYSGLV 374
           SAGNDGPS+ETV+NTSPWIL+VAA+S+DRTFL +I LGNNNTFL QTL++ +EIGYSGLV
Sbjct: 331 SAGNDGPSSETVLNTSPWILNVAASSMDRTFLATIVLGNNNTFLGQTLFSGEEIGYSGLV 390

Query: 375 YPRSGLNQIIAGLCDSITLVNGSNWMSGKVVLCFSTMVGMNPIAITRAVEAVKVGNGLGL 434
           YP SGL Q +AGLC+ + LVNGS WM+GKVVLCFS  V MNPIAI+RA EAVK GNG+GL
Sbjct: 391 YPGSGLRQEVAGLCELVPLVNGSKWMAGKVVLCFSRAVAMNPIAISRAAEAVKAGNGVGL 450

Query: 435 IVARHPDDIWFTCDNDFPCFQVNFEVGTKIFYYIRATRSPMVKLSPSRTITGNPISAHIA 494
           IVARHPDDIWF C +DFPCF V+ E+G+KIFYYIRAT SP+V+L  SRTITGNPISAHIA
Sbjct: 451 IVARHPDDIWFACADDFPCFLVDLEIGSKIFYYIRATSSPLVRLGRSRTITGNPISAHIA 510

Query: 495 YFSSRGPNSVAPAILKPDIGAPGVAILVATSPFDITNDGGFTIQTGTSIATPHISAIVAL 554
           YFSSRGPNSVAPAILKPD+ APGVAIL ATSP D TND GFTIQTGTSIATPH+SAIVAL
Sbjct: 511 YFSSRGPNSVAPAILKPDVAAPGVAILAATSPLDPTNDRGFTIQTGTSIATPHVSAIVAL 570

Query: 555 LKSLHPTWSPAAIKSAIITTAWSRH-PSGVPILAEGSPHKLGNPFDYGGGIVNPNAAANP 614
           LKSLHPTWSPAAIKSAI+TTAW+ H  SGVPIL EGSP K G+PFDYGGGIVNPNAAANP
Sbjct: 571 LKSLHPTWSPAAIKSAIVTTAWNTHSSSGVPILTEGSPWKRGDPFDYGGGIVNPNAAANP 630

Query: 615 GLIYDMSTSDYISYFCSMGYKNSAISFLTEQKTECPSKPQISLLDLNLPSITVPALTNST 674
           GLIYDM+T+DYISYFCSMGY NSAIS LT+ KTECP++ QIS+LDLNLPSITVPAL+N  
Sbjct: 631 GLIYDMATADYISYFCSMGYNNSAISVLTQDKTECPTQDQISILDLNLPSITVPALSNWN 690

Query: 675 TITRTVTNVGNLTSIYKALIHPPMGINVTVDPLVLLFNATVTNLSFKVMISTLLEMDYGF 734
           T+TRTVTNVGNLTS+Y+ +I PP+G  V V+P VL FNATV  LSFKV I +LLEMDYGF
Sbjct: 691 TVTRTVTNVGNLTSVYRVVIDPPIGTRVEVEPPVLAFNATVRKLSFKVTIWSLLEMDYGF 750

Query: 735 SFGAIIWSDGVHLVTTPLSVRTQI 758
           SFG+IIWSDGVHLV +PLSVR QI
Sbjct: 751 SFGSIIWSDGVHLVQSPLSVRIQI 765

BLAST of CcUC07G135880.1 vs. NCBI nr
Match: XP_022136958.1 (subtilisin-like protease SBT3.5 isoform X1 [Momordica charantia])

HSP 1 Score: 1149.8 bits (2973), Expect = 0.0e+00
Identity = 578/754 (76.66%), Postives = 649/754 (86.07%), Query Frame = 0

Query: 15  QLLHIVHLEHNQHDPNSSIVEIHHNFLSTLLGSKKKASKSMVYSYTHAFSGFAAKLTKSQ 74
           +LLHIV+L   +          HH+ L+T+LGSKKKASK+ VYSY HAFSGFAAKLTKSQ
Sbjct: 31  ELLHIVYLGEKKQ---------HHDLLATILGSKKKASKATVYSYKHAFSGFAAKLTKSQ 90

Query: 75  AYQLSEMRGVVRVLPSVLYDLHTTRSWDFLGLSPSPSPSNLLQMSNLGDGIIIGVVDTGI 134
           A+QL+E+ GV+RVLP+VLYDLHTTRSWDFLG+SPSP   NLL  SNLGDGIIIG++D+GI
Sbjct: 91  AHQLAEIPGVLRVLPNVLYDLHTTRSWDFLGVSPSPYSPNLLHSSNLGDGIIIGIIDSGI 150

Query: 135 WPETEAFDDQGLGPVPVRWKGECQSGEQFKATRDCNRKIIGAKWYVDGFIAEFGEFNRSA 194
           WPE+EAF+D GLGPVP RW+G C+SGE+F A+R+CNRKIIGA+WYVDGF AEFGEFNRS+
Sbjct: 151 WPESEAFNDDGLGPVPRRWRGTCKSGEKFNASRNCNRKIIGARWYVDGFKAEFGEFNRSS 210

Query: 195 VKEFMSPRDSNGHGTQTASTAAGSFVKNVSYRGLGGGTARGGAPAARLAVYKACWSLPGG 254
             EF+SPRDSNGHGTQTASTAAGSFV NVSYRGL GGT RGGAP ARLA+YKACWSLP G
Sbjct: 211 ATEFISPRDSNGHGTQTASTAAGSFVSNVSYRGLAGGTLRGGAPRARLAMYKACWSLPSG 270

Query: 255 GGQCSAADVLKGMDDAVHDGVDVLSLSLGKSAPLFAEVDEENVVAIGSFRANMRNISVVC 314
           GGQC+AADVLK +DDAV D VDVLSLSLGKS PLF EVDEENVVAIGSF A  RNI VVC
Sbjct: 271 GGQCAAADVLKALDDAVQDRVDVLSLSLGKSVPLFPEVDEENVVAIGSFHAISRNIPVVC 330

Query: 315 SAGNDGPSAETVINTSPWILSVAATSIDRTFLGSITLGNNNTFL----------SQTLYT 374
           SAGNDGPS+ETV+NTSPWIL+VAA+S+DRTFL +I LGNNNTFL           QTL++
Sbjct: 331 SAGNDGPSSETVLNTSPWILNVAASSMDRTFLATIVLGNNNTFLVGLIWPYLLQGQTLFS 390

Query: 375 RKEIGYSGLVYPRSGLNQIIAGLCDSITLVNGSNWMSGKVVLCFSTMVGMNPIAITRAVE 434
            +EIGYSGLVYP SGL Q +AGLC+ + LVNGS WM+GKVVLCFS  V MNPIAI+RA E
Sbjct: 391 GEEIGYSGLVYPGSGLRQEVAGLCELVPLVNGSKWMAGKVVLCFSRAVAMNPIAISRAAE 450

Query: 435 AVKVGNGLGLIVARHPDDIWFTCDNDFPCFQVNFEVGTKIFYYIRATRSPMVKLSPSRTI 494
           AVK GNG+GLIVARHPDDIWF C +DFPCF V+ E+G+KIFYYIRAT SP+V+L  SRTI
Sbjct: 451 AVKAGNGVGLIVARHPDDIWFACADDFPCFLVDLEIGSKIFYYIRATSSPLVRLGRSRTI 510

Query: 495 TGNPISAHIAYFSSRGPNSVAPAILKPDIGAPGVAILVATSPFDITNDGGFTIQTGTSIA 554
           TGNPISAHIAYFSSRGPNSVAPAILKPD+ APGVAIL ATSP D TND GFTIQTGTSIA
Sbjct: 511 TGNPISAHIAYFSSRGPNSVAPAILKPDVAAPGVAILAATSPLDPTNDRGFTIQTGTSIA 570

Query: 555 TPHISAIVALLKSLHPTWSPAAIKSAIITTAWSRH-PSGVPILAEGSPHKLGNPFDYGGG 614
           TPH+SAIVALLKSLHPTWSPAAIKSAI+TTAW+ H  SGVPIL EGSP K G+PFDYGGG
Sbjct: 571 TPHVSAIVALLKSLHPTWSPAAIKSAIVTTAWNTHSSSGVPILTEGSPWKRGDPFDYGGG 630

Query: 615 IVNPNAAANPGLIYDMSTSDYISYFCSMGYKNSAISFLTEQKTECPSKPQISLLDLNLPS 674
           IVNPNAAANPGLIYDM+T+DYISYFCSMGY NSAIS LT+ KTECP++ QIS+LDLNLPS
Sbjct: 631 IVNPNAAANPGLIYDMATADYISYFCSMGYNNSAISVLTQDKTECPTQDQISILDLNLPS 690

Query: 675 ITVPALTNSTTITRTVTNVGNLTSIYKALIHPPMGINVTVDPLVLLFNATVTNLSFKVMI 734
           ITVPAL+N  T+TRTVTNVGNLTS+Y+ +I PP+G  V V+P VL FNATV  LSFKV I
Sbjct: 691 ITVPALSNWNTVTRTVTNVGNLTSVYRVVIDPPIGTRVEVEPPVLAFNATVRKLSFKVTI 750

Query: 735 STLLEMDYGFSFGAIIWSDGVHLVTTPLSVRTQI 758
            +LLEMDYGFSFG+IIWSDGVHLV +PLSVR QI
Sbjct: 751 WSLLEMDYGFSFGSIIWSDGVHLVQSPLSVRIQI 775

BLAST of CcUC07G135880.1 vs. NCBI nr
Match: XP_021679638.1 (subtilisin-like protease SBT3.3 [Hevea brasiliensis])

HSP 1 Score: 916.0 bits (2366), Expect = 2.2e-262
Identity = 456/744 (61.29%), Postives = 580/744 (77.96%), Query Frame = 0

Query: 17  LHIVHLEHNQHDPNSSIVEIHHNFLSTLLGSKKKASKSMVYSYTHAFSGFAAKLTKSQAY 76
           +HIV+L   QHD    I   HH+ L+ ++GSK+ AS+ +VYSY H FSGFAAKLT+SQA 
Sbjct: 49  VHIVYLGEKQHDDPKLITNSHHDMLADVVGSKELASQLIVYSYKHGFSGFAAKLTESQAQ 108

Query: 77  QLSEMRGVVRVLPSVLYDLHTTRSWDFLGLSPSPSPSNLLQMSNLGDGIIIGVVDTGIWP 136
           +L+E+ GVVRV+P+ L+ L TTRSWDFLGLS S SP N LQ SN+GDG+IIGV DTGIWP
Sbjct: 109 KLAELPGVVRVIPNSLHKLQTTRSWDFLGLS-SYSPVNALQRSNMGDGVIIGVFDTGIWP 168

Query: 137 ETEAFDDQGLGPVPVRWKGECQSGEQFKATRDCNRKIIGAKWYVDGFIAEFGE-FNRSAV 196
           E++AF D+GLGP+P RWKG C+SG+QF AT +CN+KIIGA+WY+DGF+AE+G+  N S  
Sbjct: 169 ESKAFSDEGLGPIPFRWKGVCKSGKQFNATLNCNKKIIGARWYIDGFLAEYGKPLNTSED 228

Query: 197 KEFMSPRDSNGHGTQTASTAAGSFVKNVSYRGLGGGTARGGAPAARLAVYKACWSLPGGG 256
            EF+SPRD+NGHGT TASTAAG+F++NVSY+GLG GT RGGAP ARLA+YK CW++   G
Sbjct: 229 LEFLSPRDANGHGTHTASTAAGAFIRNVSYKGLGPGTIRGGAPRARLAIYKVCWNV--FG 288

Query: 257 GQCSAADVLKGMDDAVHDGVDVLSLSLGKSAPLFAEVDEENVVAIGSFRANMRNISVVCS 316
           GQCS+AD+LK  D+A++DGVDVLSLS+G S PLF+++DE + +A GSF A  R I+VVC 
Sbjct: 289 GQCSSADMLKAFDEAIYDGVDVLSLSIGSSIPLFSDIDERDGIATGSFHAVARGITVVCG 348

Query: 317 AGNDGPSAETVINTSPWILSVAATSIDRTFLGSITLGNNNTFLSQTLYTRKEIGYSGLVY 376
           A NDGPSA+TV NT+PWIL+VAA+++DR F   ITLGNN TFL Q ++T KEIG+ GLVY
Sbjct: 349 AANDGPSAQTVQNTAPWILTVAASTMDRAFPTPITLGNNKTFLGQAMFTGKEIGFRGLVY 408

Query: 377 PR-SGLNQIIAGLCDSITLVNGSNWMSGKVVLCFSTMVGMNPIAITRAVEAVKVGNGLGL 436
           P  SGL+   AG+C S++L   +  ++GKVVLCF++M      A+T A E VK   G+GL
Sbjct: 409 PEASGLDPNAAGVCQSLSL--NATLVAGKVVLCFTSMA--RRAAVTSAAEVVKAAGGVGL 468

Query: 437 IVARHPDDIWFTCDNDFPCFQVNFEVGTKIFYYIRATRSPMVKLSPSRTITGNPISAHIA 496
           IVA++P D  + C  DFPC +V++E+GT+I +YIR+ RSP VKLS S+TI GNP+   +A
Sbjct: 469 IVAKNPSDALYPCSGDFPCVEVDYEIGTRILFYIRSNRSPAVKLSHSKTIVGNPLLPKVA 528

Query: 497 YFSSRGPNSVAPAILKPDIGAPGVAILVATSPFDITNDGGFTIQTGTSIATPHISAIVAL 556
           YFSSRGPNS+APAILKPDI APGV IL ATSP D   D G+ + +GTS+ATPH+S IVAL
Sbjct: 529 YFSSRGPNSIAPAILKPDITAPGVNILAATSPLDRFEDNGYAMHSGTSMATPHVSGIVAL 588

Query: 557 LKSLHPTWSPAAIKSAIITTAWSRHPSGVPILAEGSPHKLGNPFDYGGGIVNPNAAANPG 616
           LK+LHP WSPAAIKSA++TTAW  HPSG PI AEGSP KL NPFD+GGGI NPN AA+PG
Sbjct: 589 LKALHPDWSPAAIKSALVTTAWRNHPSGYPIFAEGSPQKLANPFDFGGGIANPNGAADPG 648

Query: 617 LIYDMSTSDYISYFCSMGYKNSAISFLTEQKTECPSKPQISLLDLNLPSITVPALTNSTT 676
           L+YDM T+DYI Y C MGY N+AIS LT Q TECPSK + S+LD+NLPS+T+P L  S T
Sbjct: 649 LLYDMDTADYIHYLCVMGYNNAAISRLTGQPTECPSK-EPSILDVNLPSLTIPNLRKSIT 708

Query: 677 ITRTVTNVGNLTSIYKALIHPPMGINVTVDPLVLLFNATVTNLSFKVMISTLLEMDYGFS 736
           +TRTVTN G   SIY+A+I PP GI V+V P VLLF+     ++F V I+   +++ G+ 
Sbjct: 709 LTRTVTNAGAPNSIYRAVIEPPFGIIVSVKPDVLLFSNQTKKINFTVTINATHQVNTGYY 768

Query: 737 FGAIIWSDGVHLVTTPLSVRTQIL 759
           FG++ W+DG+H+V +PLSVRT++L
Sbjct: 769 FGSLSWTDGIHMVKSPLSVRTEML 784

BLAST of CcUC07G135880.1 vs. NCBI nr
Match: XP_030951705.1 (subtilisin-like protease SBT3.5 [Quercus lobata])

HSP 1 Score: 907.5 bits (2344), Expect = 7.7e-260
Identity = 453/746 (60.72%), Postives = 582/746 (78.02%), Query Frame = 0

Query: 17  LHIVHLEHNQHDPNSSIVEIHHNFLSTLLGSKKKASKSMVYSYTHAFSGFAAKLTKSQAY 76
           +HI++L   Q+D    I ++HH+ L+ ++GS + AS  MVYSY H FSGFAAKLTKSQA 
Sbjct: 36  VHIIYLGERQYDEPKLITDLHHDMLAKVVGSNEIASDLMVYSYKHGFSGFAAKLTKSQAQ 95

Query: 77  QLSEMRGVVRVLPSVLYDLHTTRSWDFLGLSPSPSPSNLLQMSNLGDGIIIGVVDTGIWP 136
           +L+E+ GVVRV+P+ L+ L TTRSWDFLGLS S SP+N+L  SN+GDG IIGV DTG+WP
Sbjct: 96  KLAELPGVVRVIPNRLHKLQTTRSWDFLGLS-SHSPNNILHSSNMGDGAIIGVFDTGLWP 155

Query: 137 ETEAFDDQGLGPVPVRWKGECQSGEQFKATRDCNRKIIGAKWYVDGFIAEFGE-FNRSAV 196
           E+  F D+GLGPVP RW+G C+SGE+F A+  CNRKIIGA+WY++GF+AE+G+  N S  
Sbjct: 156 ESRVFRDEGLGPVPPRWRGVCKSGEKFNASTHCNRKIIGARWYINGFLAEYGKPLNTSGD 215

Query: 197 KEFMSPRDSNGHGTQTASTAAGSFVKNVSYRGLGGGTARGGAPAARLAVYKACWSLPGGG 256
           +EF+SPRD+NGHGT TASTA GSFV NVSY+GLG GT RGGAP ARLA+YK CW +   G
Sbjct: 216 QEFLSPRDANGHGTHTASTAGGSFVGNVSYKGLGSGTVRGGAPRARLAIYKVCWDIL--G 275

Query: 257 GQCSAADVLKGMDDAVHDGVDVLSLSLGKSAPLFAEVDEENVVAIGSFRANMRNISVVCS 316
           GQCS+AD+LK  DDA+HDGVDVLSLS+G S PLF++VDE + +A GSF A  R I+VVC 
Sbjct: 276 GQCSSADILKAFDDAIHDGVDVLSLSIGSSIPLFSDVDERDGIATGSFHAVARGITVVCG 335

Query: 317 AGNDGPSAETVINTSPWILSVAATSIDRTFLGSITLGNNNTFLSQTLYTRKEIGYSGLVY 376
           A N+GPSA TV NT+PWIL+VAA+++DR F  SITLGNN T L Q ++T KEIG++ L+Y
Sbjct: 336 AANEGPSAYTVHNTAPWILTVAASTMDRAFPTSITLGNNKTLLGQAIFTGKEIGFTDLIY 395

Query: 377 PRS-GLNQIIAGLCDSITLVNGSNWMSGKVVLCFSTMVGMNPIAITRAVEAVKVGNGLGL 436
             S   +   AG+C+ ++L N S  +SGKVVLCF+++      A+ +A   VK   G+G+
Sbjct: 396 LESTKFDPNAAGMCEDLSLNNIS--VSGKVVLCFTSIA--RRTALVQASGVVKEAGGVGV 455

Query: 437 IVARHPDDIWFTCDNDFPCFQVNFEVGTKIFYYIRATRSPMVKLSPSRTITGNPISAHIA 496
           I+A+ P+D  ++CD DFPC +V++E+GT+I +YIR+TRSP+VKLSPS+TI G P+S  +A
Sbjct: 456 IIAKKPNDALYSCDGDFPCIEVDYEIGTRILFYIRSTRSPLVKLSPSKTIVGKPVSPKVA 515

Query: 497 YFSSRGPNSVAPAILKPDIGAPGVAILVATSPFDITNDGGFTIQTGTSIATPHISAIVAL 556
           YFSSRGPNS+APAILKPDI APG  IL ATSP     DGG+ + +GTS++TPHIS IVAL
Sbjct: 516 YFSSRGPNSIAPAILKPDITAPGANILAATSPLGQIMDGGYAMLSGTSMSTPHISGIVAL 575

Query: 557 LKSLHPTWSPAAIKSAIITTAWSRHPSGVPILAEGSPHKLGNPFDYGGGIVNPNAAANPG 616
           LK LHP WSPAAIKSA+ITTAW   PSG+PI AEG P  L NPFD+GGGIVN N AA+PG
Sbjct: 576 LKVLHPHWSPAAIKSALITTAWQTGPSGLPIFAEGFPQNLANPFDFGGGIVNANGAADPG 635

Query: 617 LIYDMSTSDYISYFCSMGYKNSAISFLTEQKTECPSKPQISLLDLNLPSITVPALTNSTT 676
           L+YDM T+DYI Y C+M Y NSAIS LT + T CPS  ++S+LD+NLPSIT+P+L NS+T
Sbjct: 636 LVYDMGTTDYIHYLCAMAYNNSAISRLTGKSTVCPS-TKVSILDVNLPSITIPSLRNSST 695

Query: 677 ITRTVTNVGNLTSIYKALIHPPMGINVTVDPLVLLFNATVTNLSFKVMISTLLEMDYGFS 736
           ITRTVTNVG L +IY+A+I PP+GI V+V P VL+FN+T+  +SFKV +ST  ++D G+ 
Sbjct: 696 ITRTVTNVGGLKTIYRAVIDPPIGIMVSVKPDVLVFNSTIKKISFKVTVSTTHKVDTGYY 755

Query: 737 FGAIIWSDGVHLVTTPLSVRTQILHS 761
           FG++ W++GVH V +PLSVRT++L S
Sbjct: 756 FGSLTWTNGVHAVRSPLSVRTELLQS 773

BLAST of CcUC07G135880.1 vs. NCBI nr
Match: PQP94706.1 (subtilisin-like protease SBT3.4 [Prunus yedoensis var. nudiflora])

HSP 1 Score: 905.6 bits (2339), Expect = 2.9e-259
Identity = 449/744 (60.35%), Postives = 582/744 (78.23%), Query Frame = 0

Query: 17  LHIVHLEHNQHDPNSSIVEIHHNFLSTLLGSKKKASKSMVYSYTHAFSGFAAKLTKSQAY 76
           +HIV+L   QHD    + + HH+ L+T++GSK+ AS+ MVYSY H FSGFAAKLT+SQA 
Sbjct: 32  VHIVYLGERQHDNPKLLTDTHHDLLATIVGSKELASELMVYSYRHGFSGFAAKLTESQAQ 91

Query: 77  QLSEMRGVVRVLPSVLYDLHTTRSWDFLGLSPSPSPSNLLQMSNLGDGIIIGVVDTGIWP 136
           +LSE+ GVVRV+P+ L+ L TTRSWDFLGLS S SPSN+L  S++GDG+IIGV+DTGIWP
Sbjct: 92  KLSELPGVVRVIPNSLHKLQTTRSWDFLGLS-SQSPSNILHKSSMGDGVIIGVLDTGIWP 151

Query: 137 ETEAFDDQGLGPVPVRWKGECQSGEQFKATRDCNRKIIGAKWYVDGFIAEFGE-FNRSAV 196
           E+E+F+++GLGPVP  WKG C+SG++F AT DCNRKIIGA+W++DG +AE+G+  NRS  
Sbjct: 152 ESESFNEKGLGPVPSHWKGVCESGDRFNATIDCNRKIIGARWFIDGLLAEYGKPLNRST- 211

Query: 197 KEFMSPRDSNGHGTQTASTAAGSFVKNVSYRGLGGGTARGGAPAARLAVYKACWSLPGGG 256
            EF+SPRD++GHGT T+STAAGSFV NVSY+GLG GT +GGAP ARLA+YK CW +   G
Sbjct: 212 -EFLSPRDAHGHGTHTSSTAAGSFVTNVSYKGLGHGTIKGGAPNARLAIYKVCWKVL--G 271

Query: 257 GQCSAADVLKGMDDAVHDGVDVLSLSLGKSAPLFAEVDEENVVAIGSFRANMRNISVVCS 316
           GQCS+AD+LK  D+A+HDGVDVLSLS+G S PLF+EVDE + +A GSF A  R I+VVC+
Sbjct: 272 GQCSSADILKAFDEAIHDGVDVLSLSIGFSIPLFSEVDEHDGIATGSFHAVARGITVVCA 331

Query: 317 AGNDGPSAETVINTSPWILSVAATSIDRTFLGSITLGNNNTFLSQTLYTRKEIGYSGLVY 376
           A NDGPSAETV NT+PWI++VAA+++DR+F  SITLGNN TFL   ++T  EIG++ L+Y
Sbjct: 332 AANDGPSAETVQNTAPWIITVAASTMDRSFPTSITLGNNKTFLGLAMFTGPEIGFASLIY 391

Query: 377 PRS-GLNQIIAGLCDSITLVNGSNWMSGKVVLCFSTMVGMNPIAITRAVEAVKVGNGLGL 436
           P S G++   AG+C  ++L      ++GKVVLCF+T+ GM  IAIT A  AVK   G+GL
Sbjct: 392 PESKGVDPTAAGVCQFLSL--NKTMVAGKVVLCFTTVSGM--IAITSASAAVKEAGGVGL 451

Query: 437 IVARHPDDIWFTCDNDFPCFQVNFEVGTKIFYYIRATRSPMVKLSPSRTITGNPISAHIA 496
           IVA++P D  + C+ DFPC +V++E+GT+I +YIR+TRSP+VKL P +T  G P+SA +A
Sbjct: 452 IVAKNPSDALYPCNEDFPCIEVDYEIGTRILFYIRSTRSPLVKLRPPKTFIGKPLSAKVA 511

Query: 497 YFSSRGPNSVAPAILKPDIGAPGVAILVATSPFDITNDGGFTIQTGTSIATPHISAIVAL 556
           YFSSRGPNS+ PAILKPDI APGV IL ATSP D   +GG+ + +GTS++TPH++ IVAL
Sbjct: 512 YFSSRGPNSITPAILKPDIAAPGVNILAATSPLDALGEGGYVMHSGTSMSTPHVAGIVAL 571

Query: 557 LKSLHPTWSPAAIKSAIITTAWSRHPSGVPILAEGSPHKLGNPFDYGGGIVNPNAAANPG 616
           LK++HP WSPAAIKSA++TTAW   PSG+PI AEGSP KL NPFD+GGGI+NPN AA+PG
Sbjct: 572 LKAMHPNWSPAAIKSALVTTAWRNGPSGLPIFAEGSPQKLANPFDFGGGIMNPNGAADPG 631

Query: 617 LIYDMSTSDYISYFCSMGYKNSAISFLTEQKTECPSKPQISLLDLNLPSITVPALTNSTT 676
           L+YD+  + Y+ Y C+ GY NSAIS L  Q T+CP K + S+LD+NLPSIT+P+L N  T
Sbjct: 632 LVYDIGKAGYMQYLCARGYNNSAISRLVGQNTKCPIK-KPSILDMNLPSITIPSLKNPIT 691

Query: 677 ITRTVTNVGNLTSIYKALIHPPMGINVTVDPLVLLFNATVTNLSFKVMISTLLEMDYGFS 736
           I R+VTNVG   SIY+A I PP G  V+V+P  L+FN+TV  L F + IS +  M+ G+ 
Sbjct: 692 IKRSVTNVGAPESIYRATIEPPFGTIVSVNPNALVFNSTVRKLDFMITISAIHRMNTGYY 751

Query: 737 FGAIIWSDGVHLVTTPLSVRTQIL 759
           FG++ W+DGVH+V  PLSVRT+ L
Sbjct: 752 FGSLSWADGVHVVRIPLSVRTEFL 765

BLAST of CcUC07G135880.1 vs. ExPASy Swiss-Prot
Match: Q9MAP7 (Subtilisin-like protease SBT3.5 OS=Arabidopsis thaliana OX=3702 GN=SBT3.5 PE=1 SV=1)

HSP 1 Score: 808.1 bits (2086), Expect = 8.3e-233
Identity = 406/747 (54.35%), Postives = 544/747 (72.82%), Query Frame = 0

Query: 17  LHIVHLEHNQHDPNSSIVEIHHNFLSTLLGSKKKASKSMVYSYTHAFSGFAAKLTKSQAY 76
           +HIV+L   QHD    + E HH  LS+LLGSK  A +SMVYSY H FSGFAAKLT+SQA 
Sbjct: 29  VHIVYLGEKQHDDPEFVSESHHQMLSSLLGSKVDAHESMVYSYRHGFSGFAAKLTESQAK 88

Query: 77  QLSEMRGVVRVLPSVLYDLHTTRSWDFLGLSPSPSPSNLLQMSNLGDGIIIGVVDTGIWP 136
           +L++   VV V+    Y+L TTR+WD+LGLS + +P+NLL  +N+GD +IIG +DTG+WP
Sbjct: 89  KLADSPEVVHVMADSFYELATTRTWDYLGLSVA-NPNNLLNDTNMGDQVIIGFIDTGVWP 148

Query: 137 ETEAFDDQGLGPVPVRWKGECQSGEQFKATRDCNRKIIGAKWYVDGFIAEFGEFNRSAVK 196
           E+E+F+D G+GP+P  WKG C+SGE+F +T +CNRK+IGAK++++GF+AE   FN +  +
Sbjct: 149 ESESFNDNGVGPIPSHWKGGCESGEKFIST-NCNRKLIGAKYFINGFLAENEGFNTTESR 208

Query: 197 EFMSPRDSNGHGTQTASTAAGSFVKNVSYRGLGGGTARGGAPAARLAVYKACWSLPG-GG 256
           +++S RD  GHGT TAS A GSFV N+SY+GL GG  RGGAP AR+A+YKACW +   G 
Sbjct: 209 DYISARDFIGHGTHTASIAGGSFVPNISYKGLAGGNLRGGAPRARIAIYKACWYVDQLGA 268

Query: 257 GQCSAADVLKGMDDAVHDGVDVLSLSLGKSAPLFAEVDEENVVAIGSFRANMRNISVVCS 316
             CS++D+LK MD+++HDGVDVLSLSLG   PL+ E D  + +A G+F A  + I VVC+
Sbjct: 269 VACSSSDILKAMDESMHDGVDVLSLSLGAQIPLYPETDLRDRIATGAFHAVAKGIIVVCA 328

Query: 317 AGNDGPSAETVINTSPWILSVAATSIDRTFLGSITLGNNNTFLSQTLYTRKEIGYSGLVY 376
            GN GP+A+TV+NT+PWI++VAAT++DR+F   ITLGN    L Q LYT +E+G++ LVY
Sbjct: 329 GGNSGPAAQTVLNTAPWIITVAATTLDRSFPTPITLGNRKVILGQALYTGQELGFTSLVY 388

Query: 377 PRSG--LNQIIAGLCDSITLVNGSNWMSGKVVLCFSTMVGMNPIAITRAVEAVKVGNGLG 436
           P +    N+  +G+C+ + L N +  M+GKVVLCF+T       A++RA   VK   GLG
Sbjct: 389 PENAGFTNETFSGVCERLNL-NPNRTMAGKVVLCFTTNTLFT--AVSRAASYVKAAGGLG 448

Query: 437 LIVARHPDDIWFTCDNDFPCFQVNFEVGTKIFYYIRATRSPMVKLSPSRTITGNPISAHI 496
           +I+AR+P      C +DFPC  +++E+GT +  YIR+TRSP+VK+ PSRT+ G P+   +
Sbjct: 449 VIIARNPGYNLTPCRDDFPCVAIDYELGTDVLLYIRSTRSPVVKIQPSRTLVGQPVGTKV 508

Query: 497 AYFSSRGPNSVAPAILKPDIGAPGVAILVATSPFDITNDGGFTIQTGTSIATPHISAIVA 556
           A FSSRGPNS++PAILKPDIGAPGV+IL ATSP   ++ GGF I  GTS+A P ++ +VA
Sbjct: 509 ATFSSRGPNSISPAILKPDIGAPGVSILAATSPDSNSSVGGFDILAGTSMAAPVVAGVVA 568

Query: 557 LLKSLHPTWSPAAIKSAIITTAWSRHPSGVPILAEGSPHKLGNPFDYGGGIVNPNAAANP 616
           LLK+LHP WSPAA +SAI+TTAW   P G  I AEGS  K+ +PFDYGGGIVNP  AA+P
Sbjct: 569 LLKALHPNWSPAAFRSAIVTTAWRTDPFGEQIFAEGSSRKVADPFDYGGGIVNPEKAADP 628

Query: 617 GLIYDMSTSDYISYFCSMGYKNSAISFLTEQKTECPSKPQISLLDLNLPSITVPALTNST 676
           GLIYDM   DYI Y CS GY +S+I+ L    T C S P+ S+LD+NLPSIT+P L +  
Sbjct: 629 GLIYDMGPRDYILYLCSAGYNDSSITQLVGNVTVC-STPKTSVLDVNLPSITIPDLKDEV 688

Query: 677 TITRTVTNVGNLTSIYKALIHPPMGINVTVDPLVLLFNATVTNLSFKVMISTLLEMDYGF 736
           T+TRTVTNVG + S+YK ++ PP+GI V V P  L+FN+   N+SF V +ST  +++ GF
Sbjct: 689 TLTRTVTNVGTVDSVYKVVVEPPLGIQVVVAPETLVFNSKTKNVSFTVRVSTTHKINTGF 748

Query: 737 SFGAIIWSDGVHLVTTPLSVRTQILHS 761
            FG +IW+D +H VT P+SVRTQIL +
Sbjct: 749 YFGNLIWTDSMHNVTIPVSVRTQILQN 769

BLAST of CcUC07G135880.1 vs. ExPASy Swiss-Prot
Match: Q9SZY3 (Subtilisin-like protease SBT3.8 OS=Arabidopsis thaliana OX=3702 GN=SBT3.8 PE=3 SV=1)

HSP 1 Score: 801.6 bits (2069), Expect = 7.8e-231
Identity = 407/747 (54.48%), Postives = 536/747 (71.75%), Query Frame = 0

Query: 17  LHIVHLEHNQHDPNSSIVEIHHNFLSTLLGSKKKASKSMVYSYTHAFSGFAAKLTKSQAY 76
           +HIV+L   QHD    + E HH  L +LLGSK+ A  SMV+SY H FSGFAAKLTKSQA 
Sbjct: 30  VHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDAHSSMVHSYRHGFSGFAAKLTKSQAK 89

Query: 77  QLSEMRGVVRVLPSVLYDLHTTRSWDFLGLSPSPSPSNLLQMSNLGDGIIIGVVDTGIWP 136
           +L+++  VV V P   Y L TTR+WD+LGLS + +P NLL  +N+G+ +IIG+VD+G+WP
Sbjct: 90  KLADLPEVVHVTPDSFYQLDTTRTWDYLGLSVA-NPKNLLNDTNMGEEVIIGIVDSGVWP 149

Query: 137 ETEAFDDQGLGPVPVRWKGECQSGEQFKATRDCNRKIIGAKWYVDGFIAEFGEFNRSAVK 196
           E+E F+D G+GPVP  WKG C SGE F +++ CN+K+IGAK++++GF+A    FN +   
Sbjct: 150 ESEVFNDNGIGPVPSHWKGGCVSGENFTSSQ-CNKKLIGAKYFINGFLATHESFNSTESL 209

Query: 197 EFMSPRDSNGHGTQTASTAAGSFVKNVSYRGLGGGTARGGAPAARLAVYKACWSLPGGG- 256
           +F+SPRD +GHGT  A+ A GS+V ++SY+GL GGT RGGAP AR+A+YKACW L     
Sbjct: 210 DFISPRDRSGHGTHVATIAGGSYVPSISYKGLAGGTVRGGAPRARIAMYKACWYLDRFDI 269

Query: 257 GQCSAADVLKGMDDAVHDGVDVLSLSLGKSAPLFAEVDEENVVAIGSFRANMRNISVVCS 316
             CS+AD+LK MD+A+HDGVDVLSLS+G   P F E D   V+A G+F A ++ I+VVCS
Sbjct: 270 NTCSSADILKAMDEAMHDGVDVLSLSIGYRFPYFPETDVRAVIATGAFHAVLKGITVVCS 329

Query: 317 AGNDGPSAETVINTSPWILSVAATSIDRTFLGSITLGNNNTFLSQTLYTRKEIGYSGLVY 376
            GN GP+A+TV NT+PWIL+VAAT++DR+F   ITLGNN   L Q +YT  E+G++ LVY
Sbjct: 330 GGNSGPAAQTVGNTAPWILTVAATTLDRSFPTPITLGNNKLILGQAMYTGPELGFTSLVY 389

Query: 377 PRS--GLNQIIAGLCDSITLVNGSNWMSGKVVLCFSTMVGMNPIAITRAVEAVKVGNGLG 436
           P +    N+  +G C+ +   N ++ M+GKVVLCF+T      I ++ AV  VK   GLG
Sbjct: 390 PENPGNSNESFSGDCE-LLFFNSNHTMAGKVVLCFTT--STRYITVSSAVSYVKEAGGLG 449

Query: 437 LIVARHPDDIWFTCDNDFPCFQVNFEVGTKIFYYIRATRSPMVKLSPSRTITGNPISAHI 496
           +IVAR+P D    C++DFPC  V++E+GT I  YIR+T  P+VK+ PS+T+ G P+   +
Sbjct: 450 VIVARNPGDNLSPCEDDFPCVAVDYELGTDILLYIRSTGLPVVKIQPSKTLVGQPVGTKV 509

Query: 497 AYFSSRGPNSVAPAILKPDIGAPGVAILVATSPFDITNDGGFTIQTGTSIATPHISAIVA 556
           A FSSRGPNS+ PAILKPDI APGV+IL AT+     ND GF   +GTS+A P IS +VA
Sbjct: 510 ADFSSRGPNSIEPAILKPDIAAPGVSILAATTTNKTFNDRGFIFLSGTSMAAPTISGVVA 569

Query: 557 LLKSLHPTWSPAAIKSAIITTAWSRHPSGVPILAEGSPHKLGNPFDYGGGIVNPNAAANP 616
           LLK+LH  WSPAAI+SAI+TTAW   P G  I AEGSP KL +PFDYGGG+VNP  AA P
Sbjct: 570 LLKALHRDWSPAAIRSAIVTTAWRTDPFGEQIFAEGSPRKLADPFDYGGGLVNPEKAAKP 629

Query: 617 GLIYDMSTSDYISYFCSMGYKNSAISFLTEQKTECPSKPQISLLDLNLPSITVPALTNST 676
           GL+YD+   DY+ Y CS+GY  ++IS L  + T C S P+ S+LD NLPSIT+P L +  
Sbjct: 630 GLVYDLGLEDYVLYMCSVGYNETSISQLVGKGTVC-SNPKPSVLDFNLPSITIPNLKDEV 689

Query: 677 TITRTVTNVGNLTSIYKALIHPPMGINVTVDPLVLLFNATVTNLSFKVMISTLLEMDYGF 736
           T+TRT+TNVG L S+YK +I PP+GI VTV P  LLFN+T   +SFKV +ST  +++ G+
Sbjct: 690 TLTRTLTNVGQLESVYKVVIEPPIGIQVTVTPETLLFNSTTKRVSFKVKVSTTHKINTGY 749

Query: 737 SFGAIIWSDGVHLVTTPLSVRTQILHS 761
            FG++ WSD +H VT PLSVRTQIL +
Sbjct: 750 FFGSLTWSDSLHNVTIPLSVRTQILQN 770

BLAST of CcUC07G135880.1 vs. ExPASy Swiss-Prot
Match: Q9MAP5 (Subtilisin-like protease SBT3.3 OS=Arabidopsis thaliana OX=3702 GN=SBT3.3 PE=2 SV=1)

HSP 1 Score: 795.0 bits (2052), Expect = 7.3e-229
Identity = 406/753 (53.92%), Postives = 540/753 (71.71%), Query Frame = 0

Query: 11  TQFDQLLHIVHLEHNQHDPNSSIVEIHHNFLSTLLGSKKKASKSMVYSYTHAFSGFAAKL 70
           ++ +  +HIV+L   +H     + E HH  L++LLGSKK A  SMVYSY H FSGFAAKL
Sbjct: 26  SETESKVHIVYLGEKKHHDPEFVTESHHQMLASLLGSKKDADDSMVYSYRHGFSGFAAKL 85

Query: 71  TKSQAYQLSEMRGVVRVLPSVLYDLHTTRSWDFLGLSPSPSPSNLLQMSNLGDGIIIGVV 130
           TKSQA +++++  VV V+P   ++L TTR+W++LGLS S +P NLL  +N+GD +IIGV+
Sbjct: 86  TKSQAKKIADLPEVVHVIPDGFHELATTRTWEYLGLS-SANPKNLLNDTNMGDQVIIGVI 145

Query: 131 DTGIWPETEAFDDQGLGPVPVRWKGECQSGEQFKATRDCNRKIIGAKWYVDGFIAEFGEF 190
           DTG+WPE+E+F+D G+GP+P +WKG C+SGE F++T DCNRK+IGAK++++GF+AE   F
Sbjct: 146 DTGVWPESESFNDNGVGPIPRKWKGGCESGENFRST-DCNRKLIGAKYFINGFLAENKGF 205

Query: 191 NRSAVKEFMSPRDSNGHGTQTASTAAGSFVKNVSYRGLGGGTARGGAPAARLAVYKACWS 250
           N +  ++++S RD +GHGT  AS A GSFV NVSY+GL GGT RGGAP AR+A+YKACW 
Sbjct: 206 NTTESRDYISARDFDGHGTHVASIAGGSFVPNVSYKGLAGGTLRGGAPRARIAMYKACWF 265

Query: 251 LPG-GGGQCSAADVLKGMDDAVHDGVDVLSLSLGKSAPLFAEVDEENVVAIGSFRANMRN 310
                G  CS +D++K +D+A+HDGVDVLS+SL    PL +E D  +  A G F A  + 
Sbjct: 266 HEELKGVTCSDSDIMKAIDEAIHDGVDVLSISLVGQIPLNSETDIRDEFATGLFHAVAKG 325

Query: 311 ISVVCSAGNDGPSAETVINTSPWILSVAATSIDRTFLGSITLGNNNTFLSQTLYTRKEIG 370
           I VVC+ GNDGP+A+TV+N +PWIL+VAAT++DR+F   ITLGNN   L Q  YT  E+G
Sbjct: 326 IVVVCAGGNDGPAAQTVVNIAPWILTVAATTLDRSFPTPITLGNNKVILGQATYTGPELG 385

Query: 371 YSGLVYPRSGL--NQIIAGLCDSITLVNGSNWMSGKVVLCFSTMVGMNPIAITRAVEAVK 430
            + LVYP +    N+  +G+C+S+ L N +  M+ KVVLCF+        AI+RA   VK
Sbjct: 386 LTSLVYPENARNNNETFSGVCESLNL-NPNYTMAMKVVLCFT--ASRTNAAISRAASFVK 445

Query: 431 VGNGLGLIVARHPDDIWFTCDNDFPCFQVNFEVGTKIFYYIRATRSPMVKLSPSRTITGN 490
              GLGLI++R+P      C++DFPC  V++E+GT I  YIR+TRSP+VK+  SRT++G 
Sbjct: 446 AAGGLGLIISRNPVYTLSPCNDDFPCVAVDYELGTDILSYIRSTRSPVVKIQRSRTLSGQ 505

Query: 491 PISAHIAYFSSRGPNSVAPAILKPDIGAPGVAILVATSPFDITNDGGFTIQTGTSIATPH 550
           P+   +  FSSRGPNS++PAILKPDI APGV IL ATSP D  N GGF + +GTS+ATP 
Sbjct: 506 PVGTKVVNFSSRGPNSMSPAILKPDIAAPGVRILAATSPNDTLNVGGFAMLSGTSMATPV 565

Query: 551 ISAIVALLKSLHPTWSPAAIKSAIITTAWSRHPSGVPILAEGSPHKLGNPFDYGGGIVNP 610
           IS ++ALLK+LHP WSPAA +SAI+TTAW   P G  I AEGS  K+ +PFDYGGGIVNP
Sbjct: 566 ISGVIALLKALHPEWSPAAFRSAIVTTAWRTDPFGEQIFAEGSSRKVSDPFDYGGGIVNP 625

Query: 611 NAAANPGLIYDMSTSDYISYFCSMGYKNSAISFLTEQKTECPSKPQISLLDLNLPSITVP 670
             AA PGLIYDM   DYI Y CS GY +S+IS L  Q T C S P+ S+LD+NLPSIT+P
Sbjct: 626 EKAAEPGLIYDMGPQDYILYLCSAGYNDSSISQLVGQITVC-SNPKPSVLDVNLPSITIP 685

Query: 671 ALTNSTTITRTVTNVGNLTSIYKALIHPPMGINVTVDPLVLLFNATVTNLSFKVMISTLL 730
            L +  T+TRTVTNVG + S+YK  + PP+G+ V V P  L+FN+   ++SF V +ST  
Sbjct: 686 NLKDEVTLTRTVTNVGLVDSVYKVSVEPPLGVRVVVTPETLVFNSKTISVSFTVRVSTTH 745

Query: 731 EMDYGFSFGAIIWSDGVHLVTTPLSVRTQILHS 761
           +++ G+ FG++ W+D VH V  PLSVRTQIL +
Sbjct: 746 KINTGYYFGSLTWTDSVHNVVIPLSVRTQILQN 772

BLAST of CcUC07G135880.1 vs. ExPASy Swiss-Prot
Match: Q9ZSB0 (Subtilisin-like protease SBT3.9 OS=Arabidopsis thaliana OX=3702 GN=SBT3.9 PE=3 SV=1)

HSP 1 Score: 790.0 bits (2039), Expect = 2.3e-227
Identity = 407/749 (54.34%), Postives = 532/749 (71.03%), Query Frame = 0

Query: 10  FTQFDQLLHIVHLEHNQHDPNSSIVEIHHNFLSTLLGSKKKASKSMVYSYTHAFSGFAAK 69
           F   +  +++V+L   +HD   S+ E HH  L +LLGSK+    S+VYSY H FSGFAAK
Sbjct: 22  FVVAESKVYVVYLGEKEHDNPESVTESHHQMLWSLLGSKEAVLDSIVYSYRHGFSGFAAK 81

Query: 70  LTKSQAYQLSEMRGVVRVLPSVLYDLHTTRSWDFLGLSPSPSPSNLLQMSNLGDGIIIGV 129
           LT+SQA Q+SE+  VV+V+P+ LY++ TTR+WD+LG+SP  S S LLQ +N+G  +I+GV
Sbjct: 82  LTESQAQQISELPEVVQVIPNTLYEMTTTRTWDYLGVSPGNSDS-LLQKANMGYNVIVGV 141

Query: 130 VDTGIWPETEAFDDQGLGPVPVRWKGECQSGEQFKATRDCNRKIIGAKWYVDGFIAEFGE 189
           +D+G+WPE+E F+D+G GP+P RWKG C+SGE F A+  CNRK+IGAK++VDG +AEFG 
Sbjct: 142 IDSGVWPESEMFNDKGFGPIPSRWKGGCESGELFNASIHCNRKLIGAKYFVDGLVAEFGV 201

Query: 190 FNRSAVKEFMSPRDSNGHGTQTASTAAGSFVKNVSYRGLGGGTARGGAPAARLAVYKACW 249
            NR+   E++SPRD  GHGT  AST  GSF+ NVSY GLG GTARGGAP   +AVYKACW
Sbjct: 202 VNRTQNPEYLSPRDFAGHGTHVASTIGGSFLPNVSYVGLGRGTARGGAPGVHIAVYKACW 261

Query: 250 SLPGGGGQCSAADVLKGMDDAVHDGVDVLSLSLGKSAPLFAEVDEENVVAIGSFRANMRN 309
           S     G CS ADVLK MD+A+HDGVD+LSLSLG S PLF E +     ++G+F A  + 
Sbjct: 262 S-----GYCSGADVLKAMDEAIHDGVDILSLSLGPSVPLFPETEH---TSVGAFHAVAKG 321

Query: 310 ISVVCSAGNDGPSAETVINTSPWILSVAATSIDRTFLGSITLGNNNTFLSQTLYTRKEIG 369
           I VV +AGN GP+A+T+ N +PW+L+VAAT+ DR+F  +ITLGNN T L Q +Y   E+G
Sbjct: 322 IPVVIAAGNAGPTAQTISNVAPWVLTVAATTQDRSFPTAITLGNNITILGQAIYGGPELG 381

Query: 370 YSGLVYPRSGLNQIIAGLCDSITLVNGSNWMSGKVVLCFSTMVGMNPIAITRAVEAVKVG 429
           + GL YP S L    +G C+ ++  N ++ M GKVVLCF+     N      A+ AV   
Sbjct: 382 FVGLTYPESPL----SGDCEKLS-ANPNSTMEGKVVLCFAASTPSN-----AAIAAVINA 441

Query: 430 NGLGLIVARHPDDIWFTCDNDFPCFQVNFEVGTKIFYYIRATRSPMVKLSPSRTITGNPI 489
            GLGLI+A++P     T    FP   ++FE+GT I +YIR+TRSP+VK+  S+T+ G  +
Sbjct: 442 GGLGLIMAKNPTHS-LTPTRKFPWVSIDFELGTDILFYIRSTRSPIVKIQASKTLFGQSV 501

Query: 490 SAHIAYFSSRGPNSVAPAILKPDIGAPGVAILVATSPFDITNDGGFTIQTGTSIATPHIS 549
           S  +A FSSRGPNSV+PAILKPDI APGV IL A SP    NDGGF + +GTS+ATP +S
Sbjct: 502 STKVATFSSRGPNSVSPAILKPDIAAPGVNILAAISPNSSINDGGFAMMSGTSMATPVVS 561

Query: 550 AIVALLKSLHPTWSPAAIKSAIITTAWSRHPSGVPILAEGSPHKLGNPFDYGGGIVNPNA 609
            +V LLKSLHP WSP+AIKSAI+TTAW   PSG PI A+GS  KL +PFDYGGG++NP  
Sbjct: 562 GVVVLLKSLHPDWSPSAIKSAIVTTAWRTDPSGEPIFADGSSRKLADPFDYGGGLINPEK 621

Query: 610 AANPGLIYDMSTSDYISYFCSMGYKNSAISFLTEQKTECPSKPQISLLDLNLPSITVPAL 669
           A  PGLIYDM+T DY+ Y CS+ Y + +IS +  + T CP+ P+ S+LDLNLPSIT+P L
Sbjct: 622 AVKPGLIYDMTTDDYVMYMCSVDYSDISISRVLGKITVCPN-PKPSVLDLNLPSITIPNL 681

Query: 670 TNSTTITRTVTNVGNLTSIYKALIHPPMGINVTVDPLVLLFNATVTNLSFKVMISTLLEM 729
               T+TRTVTNVG + S+YK +I PP GINV V P  L+F+ T T  SF V +ST  ++
Sbjct: 682 RGEVTLTRTVTNVGPVNSVYKVVIDPPTGINVAVTPAELVFDYTTTKRSFTVRVSTTHKV 741

Query: 730 DYGFSFGAIIWSDGVHLVTTPLSVRTQIL 759
           + G+ FG++ W+D +H V  P+SVRTQIL
Sbjct: 742 NTGYYFGSLTWTDNMHNVAIPVSVRTQIL 749

BLAST of CcUC07G135880.1 vs. ExPASy Swiss-Prot
Match: F4HPF1 (Subtilisin-like protease SBT3.4 OS=Arabidopsis thaliana OX=3702 GN=SBT3.4 PE=3 SV=1)

HSP 1 Score: 786.2 bits (2029), Expect = 3.4e-226
Identity = 401/747 (53.68%), Postives = 533/747 (71.35%), Query Frame = 0

Query: 17  LHIVHLEHNQHDPNSSIVEIHHNFLSTLLGSKKKASKSMVYSYTHAFSGFAAKLTKSQAY 76
           +HIV+L   QHD    + E HH  LS+LLGSK  A +SMVYSY H FSGFAAKLTKSQA 
Sbjct: 29  VHIVYLGEKQHDDPKFVTESHHQMLSSLLGSKDDAHESMVYSYRHGFSGFAAKLTKSQAK 88

Query: 77  QLSEMRGVVRVLPSVLYDLHTTRSWDFLGLSPSPSPSNLLQMSNLGDGIIIGVVDTGIWP 136
           ++++   V+ V+P   Y+L TTR WD+LG S   S  NL+  +N+GD  IIGV+DTG+WP
Sbjct: 89  KIADSPEVIHVIPDSYYELATTRIWDYLGPSADNS-KNLVSDTNMGDQTIIGVIDTGVWP 148

Query: 137 ETEAFDDQGLGPVPVRWKGECQSGEQFKATRDCNRKIIGAKWYVDGFIAEFGEFNRSAVK 196
           E+E+F+D G+GPVP  WKG C+ GE F +T +CNRK+IGAK++++GF+AE  +FN +   
Sbjct: 149 ESESFNDYGVGPVPSHWKGGCEPGENFIST-NCNRKLIGAKYFINGFLAE-NQFNATESP 208

Query: 197 EFMSPRDSNGHGTQTASTAAGSFVKNVSYRGLGGGTARGGAPAARLAVYKACWSLPG-GG 256
           +++S RD +GHGT  AS A GSFV NVSY+GLG GT RGGAP AR+A+YKACW +    G
Sbjct: 209 DYISARDFDGHGTHVASIAGGSFVPNVSYKGLGRGTLRGGAPRARIAMYKACWYINELDG 268

Query: 257 GQCSAADVLKGMDDAVHDGVDVLSLSLGKSAPLFAEVDEENVVAIGSFRANMRNISVVCS 316
             CS +D++K +D+A+HDGVDVLS+SLG   PL +E D  + +A G+F A  + I VVC+
Sbjct: 269 VTCSFSDIMKAIDEAIHDGVDVLSISLGGRVPLNSETDLRDGIATGAFHAVAKGIVVVCA 328

Query: 317 AGNDGPSAETVINTSPWILSVAATSIDRTFLGSITLGNNNTFLSQTLYTRKEIGYSGLVY 376
            GN GPS++TV+NT+PWIL+VAAT++DR+F   I LGNN   L Q +Y   E+G++ LVY
Sbjct: 329 GGNAGPSSQTVVNTAPWILTVAATTLDRSFATPIILGNNQVILGQAMYIGPELGFTSLVY 388

Query: 377 PRSGLNQI--IAGLCDSITLVNGSNWMSGKVVLCFSTMVGMNPIAITRAVEAVKVGNGLG 436
           P    N I   +G+C+S+ L N +  M+GKVVLCF+T    +   ++ A   VK   GLG
Sbjct: 389 PEDPGNSIDTFSGVCESLNL-NSNRTMAGKVVLCFTT--ARDFTVVSTAASIVKAAGGLG 448

Query: 437 LIVARHPDDIWFTCDNDFPCFQVNFEVGTKIFYYIRATRSPMVKLSPSRTITGNPISAHI 496
           LI+AR+P      C +DFPC  ++ E+GT I +YIR T SP+VK+ PSRT+ G P+   +
Sbjct: 449 LIIARNPGYNLAPCSDDFPCVAIDNELGTDILFYIRYTGSPVVKIQPSRTLVGEPVGTKV 508

Query: 497 AYFSSRGPNSVAPAILKPDIGAPGVAILVATSPFDITNDGGFTIQTGTSIATPHISAIVA 556
           A FSSRGPNS++PAILKPDI APGV+IL ATSP D  N GGF +++GTS+A P IS ++A
Sbjct: 509 ATFSSRGPNSISPAILKPDIAAPGVSILAATSPNDTLNAGGFVMRSGTSMAAPVISGVIA 568

Query: 557 LLKSLHPTWSPAAIKSAIITTAWSRHPSGVPILAEGSPHKLGNPFDYGGGIVNPNAAANP 616
           LLKSLHP WSPAA +SAI+TTAW   P G  I AE S  K+ +PFDYGGG+VNP  AA P
Sbjct: 569 LLKSLHPDWSPAAFRSAIVTTAWRTDPFGEQIAAESSSLKVPDPFDYGGGLVNPEKAAEP 628

Query: 617 GLIYDMSTSDYISYFCSMGYKNSAISFLTEQKTECPSKPQISLLDLNLPSITVPALTNST 676
           GLI DM + DY+ Y CS GY +S+IS L  + T C S P+ S+LD+NLPSIT+P L +  
Sbjct: 629 GLILDMDSQDYVLYLCSAGYNDSSISRLVGKVTVC-SNPKPSVLDINLPSITIPNLKDEV 688

Query: 677 TITRTVTNVGNLTSIYKALIHPPMGINVTVDPLVLLFNATVTNLSFKVMISTLLEMDYGF 736
           T+TRTVTNVG + S+YK L+ PP+GI V V P  L+FN+   ++SF V++ST  +++ GF
Sbjct: 689 TLTRTVTNVGPVDSVYKVLVEPPLGIQVVVTPETLVFNSKTKSVSFTVIVSTTHKINTGF 748

Query: 737 SFGAIIWSDGVHLVTTPLSVRTQILHS 761
            FG++ W+D +H V  P+SVRTQIL +
Sbjct: 749 YFGSLTWTDSIHNVVIPVSVRTQILQN 768

BLAST of CcUC07G135880.1 vs. ExPASy TrEMBL
Match: A0A6J1C5D1 (subtilisin-like protease SBT3.5 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111008525 PE=3 SV=1)

HSP 1 Score: 1157.9 bits (2994), Expect = 0.0e+00
Identity = 578/744 (77.69%), Postives = 649/744 (87.23%), Query Frame = 0

Query: 15  QLLHIVHLEHNQHDPNSSIVEIHHNFLSTLLGSKKKASKSMVYSYTHAFSGFAAKLTKSQ 74
           +LLHIV+L   +          HH+ L+T+LGSKKKASK+ VYSY HAFSGFAAKLTKSQ
Sbjct: 31  ELLHIVYLGEKKQ---------HHDLLATILGSKKKASKATVYSYKHAFSGFAAKLTKSQ 90

Query: 75  AYQLSEMRGVVRVLPSVLYDLHTTRSWDFLGLSPSPSPSNLLQMSNLGDGIIIGVVDTGI 134
           A+QL+E+ GV+RVLP+VLYDLHTTRSWDFLG+SPSP   NLL  SNLGDGIIIG++D+GI
Sbjct: 91  AHQLAEIPGVLRVLPNVLYDLHTTRSWDFLGVSPSPYSPNLLHSSNLGDGIIIGIIDSGI 150

Query: 135 WPETEAFDDQGLGPVPVRWKGECQSGEQFKATRDCNRKIIGAKWYVDGFIAEFGEFNRSA 194
           WPE+EAF+D GLGPVP RW+G C+SGE+F A+R+CNRKIIGA+WYVDGF AEFGEFNRS+
Sbjct: 151 WPESEAFNDDGLGPVPRRWRGTCKSGEKFNASRNCNRKIIGARWYVDGFKAEFGEFNRSS 210

Query: 195 VKEFMSPRDSNGHGTQTASTAAGSFVKNVSYRGLGGGTARGGAPAARLAVYKACWSLPGG 254
             EF+SPRDSNGHGTQTASTAAGSFV NVSYRGL GGT RGGAP ARLA+YKACWSLP G
Sbjct: 211 ATEFISPRDSNGHGTQTASTAAGSFVSNVSYRGLAGGTLRGGAPRARLAMYKACWSLPSG 270

Query: 255 GGQCSAADVLKGMDDAVHDGVDVLSLSLGKSAPLFAEVDEENVVAIGSFRANMRNISVVC 314
           GGQC+AADVLK +DDAV D VDVLSLSLGKS PLF EVDEENVVAIGSF A  RNI VVC
Sbjct: 271 GGQCAAADVLKALDDAVQDRVDVLSLSLGKSVPLFPEVDEENVVAIGSFHAISRNIPVVC 330

Query: 315 SAGNDGPSAETVINTSPWILSVAATSIDRTFLGSITLGNNNTFLSQTLYTRKEIGYSGLV 374
           SAGNDGPS+ETV+NTSPWIL+VAA+S+DRTFL +I LGNNNTFL QTL++ +EIGYSGLV
Sbjct: 331 SAGNDGPSSETVLNTSPWILNVAASSMDRTFLATIVLGNNNTFLGQTLFSGEEIGYSGLV 390

Query: 375 YPRSGLNQIIAGLCDSITLVNGSNWMSGKVVLCFSTMVGMNPIAITRAVEAVKVGNGLGL 434
           YP SGL Q +AGLC+ + LVNGS WM+GKVVLCFS  V MNPIAI+RA EAVK GNG+GL
Sbjct: 391 YPGSGLRQEVAGLCELVPLVNGSKWMAGKVVLCFSRAVAMNPIAISRAAEAVKAGNGVGL 450

Query: 435 IVARHPDDIWFTCDNDFPCFQVNFEVGTKIFYYIRATRSPMVKLSPSRTITGNPISAHIA 494
           IVARHPDDIWF C +DFPCF V+ E+G+KIFYYIRAT SP+V+L  SRTITGNPISAHIA
Sbjct: 451 IVARHPDDIWFACADDFPCFLVDLEIGSKIFYYIRATSSPLVRLGRSRTITGNPISAHIA 510

Query: 495 YFSSRGPNSVAPAILKPDIGAPGVAILVATSPFDITNDGGFTIQTGTSIATPHISAIVAL 554
           YFSSRGPNSVAPAILKPD+ APGVAIL ATSP D TND GFTIQTGTSIATPH+SAIVAL
Sbjct: 511 YFSSRGPNSVAPAILKPDVAAPGVAILAATSPLDPTNDRGFTIQTGTSIATPHVSAIVAL 570

Query: 555 LKSLHPTWSPAAIKSAIITTAWSRH-PSGVPILAEGSPHKLGNPFDYGGGIVNPNAAANP 614
           LKSLHPTWSPAAIKSAI+TTAW+ H  SGVPIL EGSP K G+PFDYGGGIVNPNAAANP
Sbjct: 571 LKSLHPTWSPAAIKSAIVTTAWNTHSSSGVPILTEGSPWKRGDPFDYGGGIVNPNAAANP 630

Query: 615 GLIYDMSTSDYISYFCSMGYKNSAISFLTEQKTECPSKPQISLLDLNLPSITVPALTNST 674
           GLIYDM+T+DYISYFCSMGY NSAIS LT+ KTECP++ QIS+LDLNLPSITVPAL+N  
Sbjct: 631 GLIYDMATADYISYFCSMGYNNSAISVLTQDKTECPTQDQISILDLNLPSITVPALSNWN 690

Query: 675 TITRTVTNVGNLTSIYKALIHPPMGINVTVDPLVLLFNATVTNLSFKVMISTLLEMDYGF 734
           T+TRTVTNVGNLTS+Y+ +I PP+G  V V+P VL FNATV  LSFKV I +LLEMDYGF
Sbjct: 691 TVTRTVTNVGNLTSVYRVVIDPPIGTRVEVEPPVLAFNATVRKLSFKVTIWSLLEMDYGF 750

Query: 735 SFGAIIWSDGVHLVTTPLSVRTQI 758
           SFG+IIWSDGVHLV +PLSVR QI
Sbjct: 751 SFGSIIWSDGVHLVQSPLSVRIQI 765

BLAST of CcUC07G135880.1 vs. ExPASy TrEMBL
Match: A0A6J1C8Y6 (subtilisin-like protease SBT3.5 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111008525 PE=3 SV=1)

HSP 1 Score: 1149.8 bits (2973), Expect = 0.0e+00
Identity = 578/754 (76.66%), Postives = 649/754 (86.07%), Query Frame = 0

Query: 15  QLLHIVHLEHNQHDPNSSIVEIHHNFLSTLLGSKKKASKSMVYSYTHAFSGFAAKLTKSQ 74
           +LLHIV+L   +          HH+ L+T+LGSKKKASK+ VYSY HAFSGFAAKLTKSQ
Sbjct: 31  ELLHIVYLGEKKQ---------HHDLLATILGSKKKASKATVYSYKHAFSGFAAKLTKSQ 90

Query: 75  AYQLSEMRGVVRVLPSVLYDLHTTRSWDFLGLSPSPSPSNLLQMSNLGDGIIIGVVDTGI 134
           A+QL+E+ GV+RVLP+VLYDLHTTRSWDFLG+SPSP   NLL  SNLGDGIIIG++D+GI
Sbjct: 91  AHQLAEIPGVLRVLPNVLYDLHTTRSWDFLGVSPSPYSPNLLHSSNLGDGIIIGIIDSGI 150

Query: 135 WPETEAFDDQGLGPVPVRWKGECQSGEQFKATRDCNRKIIGAKWYVDGFIAEFGEFNRSA 194
           WPE+EAF+D GLGPVP RW+G C+SGE+F A+R+CNRKIIGA+WYVDGF AEFGEFNRS+
Sbjct: 151 WPESEAFNDDGLGPVPRRWRGTCKSGEKFNASRNCNRKIIGARWYVDGFKAEFGEFNRSS 210

Query: 195 VKEFMSPRDSNGHGTQTASTAAGSFVKNVSYRGLGGGTARGGAPAARLAVYKACWSLPGG 254
             EF+SPRDSNGHGTQTASTAAGSFV NVSYRGL GGT RGGAP ARLA+YKACWSLP G
Sbjct: 211 ATEFISPRDSNGHGTQTASTAAGSFVSNVSYRGLAGGTLRGGAPRARLAMYKACWSLPSG 270

Query: 255 GGQCSAADVLKGMDDAVHDGVDVLSLSLGKSAPLFAEVDEENVVAIGSFRANMRNISVVC 314
           GGQC+AADVLK +DDAV D VDVLSLSLGKS PLF EVDEENVVAIGSF A  RNI VVC
Sbjct: 271 GGQCAAADVLKALDDAVQDRVDVLSLSLGKSVPLFPEVDEENVVAIGSFHAISRNIPVVC 330

Query: 315 SAGNDGPSAETVINTSPWILSVAATSIDRTFLGSITLGNNNTFL----------SQTLYT 374
           SAGNDGPS+ETV+NTSPWIL+VAA+S+DRTFL +I LGNNNTFL           QTL++
Sbjct: 331 SAGNDGPSSETVLNTSPWILNVAASSMDRTFLATIVLGNNNTFLVGLIWPYLLQGQTLFS 390

Query: 375 RKEIGYSGLVYPRSGLNQIIAGLCDSITLVNGSNWMSGKVVLCFSTMVGMNPIAITRAVE 434
            +EIGYSGLVYP SGL Q +AGLC+ + LVNGS WM+GKVVLCFS  V MNPIAI+RA E
Sbjct: 391 GEEIGYSGLVYPGSGLRQEVAGLCELVPLVNGSKWMAGKVVLCFSRAVAMNPIAISRAAE 450

Query: 435 AVKVGNGLGLIVARHPDDIWFTCDNDFPCFQVNFEVGTKIFYYIRATRSPMVKLSPSRTI 494
           AVK GNG+GLIVARHPDDIWF C +DFPCF V+ E+G+KIFYYIRAT SP+V+L  SRTI
Sbjct: 451 AVKAGNGVGLIVARHPDDIWFACADDFPCFLVDLEIGSKIFYYIRATSSPLVRLGRSRTI 510

Query: 495 TGNPISAHIAYFSSRGPNSVAPAILKPDIGAPGVAILVATSPFDITNDGGFTIQTGTSIA 554
           TGNPISAHIAYFSSRGPNSVAPAILKPD+ APGVAIL ATSP D TND GFTIQTGTSIA
Sbjct: 511 TGNPISAHIAYFSSRGPNSVAPAILKPDVAAPGVAILAATSPLDPTNDRGFTIQTGTSIA 570

Query: 555 TPHISAIVALLKSLHPTWSPAAIKSAIITTAWSRH-PSGVPILAEGSPHKLGNPFDYGGG 614
           TPH+SAIVALLKSLHPTWSPAAIKSAI+TTAW+ H  SGVPIL EGSP K G+PFDYGGG
Sbjct: 571 TPHVSAIVALLKSLHPTWSPAAIKSAIVTTAWNTHSSSGVPILTEGSPWKRGDPFDYGGG 630

Query: 615 IVNPNAAANPGLIYDMSTSDYISYFCSMGYKNSAISFLTEQKTECPSKPQISLLDLNLPS 674
           IVNPNAAANPGLIYDM+T+DYISYFCSMGY NSAIS LT+ KTECP++ QIS+LDLNLPS
Sbjct: 631 IVNPNAAANPGLIYDMATADYISYFCSMGYNNSAISVLTQDKTECPTQDQISILDLNLPS 690

Query: 675 ITVPALTNSTTITRTVTNVGNLTSIYKALIHPPMGINVTVDPLVLLFNATVTNLSFKVMI 734
           ITVPAL+N  T+TRTVTNVGNLTS+Y+ +I PP+G  V V+P VL FNATV  LSFKV I
Sbjct: 691 ITVPALSNWNTVTRTVTNVGNLTSVYRVVIDPPIGTRVEVEPPVLAFNATVRKLSFKVTI 750

Query: 735 STLLEMDYGFSFGAIIWSDGVHLVTTPLSVRTQI 758
            +LLEMDYGFSFG+IIWSDGVHLV +PLSVR QI
Sbjct: 751 WSLLEMDYGFSFGSIIWSDGVHLVQSPLSVRIQI 775

BLAST of CcUC07G135880.1 vs. ExPASy TrEMBL
Match: A0A7N2KV16 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 907.5 bits (2344), Expect = 3.7e-260
Identity = 453/746 (60.72%), Postives = 582/746 (78.02%), Query Frame = 0

Query: 17  LHIVHLEHNQHDPNSSIVEIHHNFLSTLLGSKKKASKSMVYSYTHAFSGFAAKLTKSQAY 76
           +HI++L   Q+D    I ++HH+ L+ ++GS + AS  MVYSY H FSGFAAKLTKSQA 
Sbjct: 36  VHIIYLGERQYDEPKLITDLHHDMLAKVVGSNEIASDLMVYSYKHGFSGFAAKLTKSQAQ 95

Query: 77  QLSEMRGVVRVLPSVLYDLHTTRSWDFLGLSPSPSPSNLLQMSNLGDGIIIGVVDTGIWP 136
           +L+E+ GVVRV+P+ L+ L TTRSWDFLGLS S SP+N+L  SN+GDG IIGV DTG+WP
Sbjct: 96  KLAELPGVVRVIPNRLHKLQTTRSWDFLGLS-SHSPNNILHSSNMGDGAIIGVFDTGLWP 155

Query: 137 ETEAFDDQGLGPVPVRWKGECQSGEQFKATRDCNRKIIGAKWYVDGFIAEFGE-FNRSAV 196
           E+  F D+GLGPVP RW+G C+SGE+F A+  CNRKIIGA+WY++GF+AE+G+  N S  
Sbjct: 156 ESRVFRDEGLGPVPPRWRGVCKSGEKFNASTHCNRKIIGARWYINGFLAEYGKPLNTSGD 215

Query: 197 KEFMSPRDSNGHGTQTASTAAGSFVKNVSYRGLGGGTARGGAPAARLAVYKACWSLPGGG 256
           +EF+SPRD+NGHGT TASTA GSFV NVSY+GLG GT RGGAP ARLA+YK CW +   G
Sbjct: 216 QEFLSPRDANGHGTHTASTAGGSFVGNVSYKGLGSGTVRGGAPRARLAIYKVCWDIL--G 275

Query: 257 GQCSAADVLKGMDDAVHDGVDVLSLSLGKSAPLFAEVDEENVVAIGSFRANMRNISVVCS 316
           GQCS+AD+LK  DDA+HDGVDVLSLS+G S PLF++VDE + +A GSF A  R I+VVC 
Sbjct: 276 GQCSSADILKAFDDAIHDGVDVLSLSIGSSIPLFSDVDERDGIATGSFHAVARGITVVCG 335

Query: 317 AGNDGPSAETVINTSPWILSVAATSIDRTFLGSITLGNNNTFLSQTLYTRKEIGYSGLVY 376
           A N+GPSA TV NT+PWIL+VAA+++DR F  SITLGNN T L Q ++T KEIG++ L+Y
Sbjct: 336 AANEGPSAYTVHNTAPWILTVAASTMDRAFPTSITLGNNKTLLGQAIFTGKEIGFTDLIY 395

Query: 377 PRS-GLNQIIAGLCDSITLVNGSNWMSGKVVLCFSTMVGMNPIAITRAVEAVKVGNGLGL 436
             S   +   AG+C+ ++L N S  +SGKVVLCF+++      A+ +A   VK   G+G+
Sbjct: 396 LESTKFDPNAAGMCEDLSLNNIS--VSGKVVLCFTSIA--RRTALVQASGVVKEAGGVGV 455

Query: 437 IVARHPDDIWFTCDNDFPCFQVNFEVGTKIFYYIRATRSPMVKLSPSRTITGNPISAHIA 496
           I+A+ P+D  ++CD DFPC +V++E+GT+I +YIR+TRSP+VKLSPS+TI G P+S  +A
Sbjct: 456 IIAKKPNDALYSCDGDFPCIEVDYEIGTRILFYIRSTRSPLVKLSPSKTIVGKPVSPKVA 515

Query: 497 YFSSRGPNSVAPAILKPDIGAPGVAILVATSPFDITNDGGFTIQTGTSIATPHISAIVAL 556
           YFSSRGPNS+APAILKPDI APG  IL ATSP     DGG+ + +GTS++TPHIS IVAL
Sbjct: 516 YFSSRGPNSIAPAILKPDITAPGANILAATSPLGQIMDGGYAMLSGTSMSTPHISGIVAL 575

Query: 557 LKSLHPTWSPAAIKSAIITTAWSRHPSGVPILAEGSPHKLGNPFDYGGGIVNPNAAANPG 616
           LK LHP WSPAAIKSA+ITTAW   PSG+PI AEG P  L NPFD+GGGIVN N AA+PG
Sbjct: 576 LKVLHPHWSPAAIKSALITTAWQTGPSGLPIFAEGFPQNLANPFDFGGGIVNANGAADPG 635

Query: 617 LIYDMSTSDYISYFCSMGYKNSAISFLTEQKTECPSKPQISLLDLNLPSITVPALTNSTT 676
           L+YDM T+DYI Y C+M Y NSAIS LT + T CPS  ++S+LD+NLPSIT+P+L NS+T
Sbjct: 636 LVYDMGTTDYIHYLCAMAYNNSAISRLTGKSTVCPS-TKVSILDVNLPSITIPSLRNSST 695

Query: 677 ITRTVTNVGNLTSIYKALIHPPMGINVTVDPLVLLFNATVTNLSFKVMISTLLEMDYGFS 736
           ITRTVTNVG L +IY+A+I PP+GI V+V P VL+FN+T+  +SFKV +ST  ++D G+ 
Sbjct: 696 ITRTVTNVGGLKTIYRAVIDPPIGIMVSVKPDVLVFNSTIKKISFKVTVSTTHKVDTGYY 755

Query: 737 FGAIIWSDGVHLVTTPLSVRTQILHS 761
           FG++ W++GVH V +PLSVRT++L S
Sbjct: 756 FGSLTWTNGVHAVRSPLSVRTELLQS 773

BLAST of CcUC07G135880.1 vs. ExPASy TrEMBL
Match: A0A314XQK5 (Subtilisin-like protease SBT3.4 OS=Prunus yedoensis var. nudiflora OX=2094558 GN=Pyn_15811 PE=3 SV=1)

HSP 1 Score: 905.6 bits (2339), Expect = 1.4e-259
Identity = 449/744 (60.35%), Postives = 582/744 (78.23%), Query Frame = 0

Query: 17  LHIVHLEHNQHDPNSSIVEIHHNFLSTLLGSKKKASKSMVYSYTHAFSGFAAKLTKSQAY 76
           +HIV+L   QHD    + + HH+ L+T++GSK+ AS+ MVYSY H FSGFAAKLT+SQA 
Sbjct: 32  VHIVYLGERQHDNPKLLTDTHHDLLATIVGSKELASELMVYSYRHGFSGFAAKLTESQAQ 91

Query: 77  QLSEMRGVVRVLPSVLYDLHTTRSWDFLGLSPSPSPSNLLQMSNLGDGIIIGVVDTGIWP 136
           +LSE+ GVVRV+P+ L+ L TTRSWDFLGLS S SPSN+L  S++GDG+IIGV+DTGIWP
Sbjct: 92  KLSELPGVVRVIPNSLHKLQTTRSWDFLGLS-SQSPSNILHKSSMGDGVIIGVLDTGIWP 151

Query: 137 ETEAFDDQGLGPVPVRWKGECQSGEQFKATRDCNRKIIGAKWYVDGFIAEFGE-FNRSAV 196
           E+E+F+++GLGPVP  WKG C+SG++F AT DCNRKIIGA+W++DG +AE+G+  NRS  
Sbjct: 152 ESESFNEKGLGPVPSHWKGVCESGDRFNATIDCNRKIIGARWFIDGLLAEYGKPLNRST- 211

Query: 197 KEFMSPRDSNGHGTQTASTAAGSFVKNVSYRGLGGGTARGGAPAARLAVYKACWSLPGGG 256
            EF+SPRD++GHGT T+STAAGSFV NVSY+GLG GT +GGAP ARLA+YK CW +   G
Sbjct: 212 -EFLSPRDAHGHGTHTSSTAAGSFVTNVSYKGLGHGTIKGGAPNARLAIYKVCWKVL--G 271

Query: 257 GQCSAADVLKGMDDAVHDGVDVLSLSLGKSAPLFAEVDEENVVAIGSFRANMRNISVVCS 316
           GQCS+AD+LK  D+A+HDGVDVLSLS+G S PLF+EVDE + +A GSF A  R I+VVC+
Sbjct: 272 GQCSSADILKAFDEAIHDGVDVLSLSIGFSIPLFSEVDEHDGIATGSFHAVARGITVVCA 331

Query: 317 AGNDGPSAETVINTSPWILSVAATSIDRTFLGSITLGNNNTFLSQTLYTRKEIGYSGLVY 376
           A NDGPSAETV NT+PWI++VAA+++DR+F  SITLGNN TFL   ++T  EIG++ L+Y
Sbjct: 332 AANDGPSAETVQNTAPWIITVAASTMDRSFPTSITLGNNKTFLGLAMFTGPEIGFASLIY 391

Query: 377 PRS-GLNQIIAGLCDSITLVNGSNWMSGKVVLCFSTMVGMNPIAITRAVEAVKVGNGLGL 436
           P S G++   AG+C  ++L      ++GKVVLCF+T+ GM  IAIT A  AVK   G+GL
Sbjct: 392 PESKGVDPTAAGVCQFLSL--NKTMVAGKVVLCFTTVSGM--IAITSASAAVKEAGGVGL 451

Query: 437 IVARHPDDIWFTCDNDFPCFQVNFEVGTKIFYYIRATRSPMVKLSPSRTITGNPISAHIA 496
           IVA++P D  + C+ DFPC +V++E+GT+I +YIR+TRSP+VKL P +T  G P+SA +A
Sbjct: 452 IVAKNPSDALYPCNEDFPCIEVDYEIGTRILFYIRSTRSPLVKLRPPKTFIGKPLSAKVA 511

Query: 497 YFSSRGPNSVAPAILKPDIGAPGVAILVATSPFDITNDGGFTIQTGTSIATPHISAIVAL 556
           YFSSRGPNS+ PAILKPDI APGV IL ATSP D   +GG+ + +GTS++TPH++ IVAL
Sbjct: 512 YFSSRGPNSITPAILKPDIAAPGVNILAATSPLDALGEGGYVMHSGTSMSTPHVAGIVAL 571

Query: 557 LKSLHPTWSPAAIKSAIITTAWSRHPSGVPILAEGSPHKLGNPFDYGGGIVNPNAAANPG 616
           LK++HP WSPAAIKSA++TTAW   PSG+PI AEGSP KL NPFD+GGGI+NPN AA+PG
Sbjct: 572 LKAMHPNWSPAAIKSALVTTAWRNGPSGLPIFAEGSPQKLANPFDFGGGIMNPNGAADPG 631

Query: 617 LIYDMSTSDYISYFCSMGYKNSAISFLTEQKTECPSKPQISLLDLNLPSITVPALTNSTT 676
           L+YD+  + Y+ Y C+ GY NSAIS L  Q T+CP K + S+LD+NLPSIT+P+L N  T
Sbjct: 632 LVYDIGKAGYMQYLCARGYNNSAISRLVGQNTKCPIK-KPSILDMNLPSITIPSLKNPIT 691

Query: 677 ITRTVTNVGNLTSIYKALIHPPMGINVTVDPLVLLFNATVTNLSFKVMISTLLEMDYGFS 736
           I R+VTNVG   SIY+A I PP G  V+V+P  L+FN+TV  L F + IS +  M+ G+ 
Sbjct: 692 IKRSVTNVGAPESIYRATIEPPFGTIVSVNPNALVFNSTVRKLDFMITISAIHRMNTGYY 751

Query: 737 FGAIIWSDGVHLVTTPLSVRTQIL 759
           FG++ W+DGVH+V  PLSVRT+ L
Sbjct: 752 FGSLSWADGVHVVRIPLSVRTEFL 765

BLAST of CcUC07G135880.1 vs. ExPASy TrEMBL
Match: A0A314UH94 (Subtilisin-like protease SBT3.4 OS=Prunus yedoensis var. nudiflora OX=2094558 GN=Pyn_29477 PE=3 SV=1)

HSP 1 Score: 904.8 bits (2337), Expect = 2.4e-259
Identity = 448/744 (60.22%), Postives = 582/744 (78.23%), Query Frame = 0

Query: 17  LHIVHLEHNQHDPNSSIVEIHHNFLSTLLGSKKKASKSMVYSYTHAFSGFAAKLTKSQAY 76
           +HIV+L   QHD    + + HH+ L+T++GSK+ AS+ MVYSY H FSGFAAKLT+SQA 
Sbjct: 32  VHIVYLGERQHDNPKLLTDTHHDLLATIVGSKELASELMVYSYRHGFSGFAAKLTESQAQ 91

Query: 77  QLSEMRGVVRVLPSVLYDLHTTRSWDFLGLSPSPSPSNLLQMSNLGDGIIIGVVDTGIWP 136
           +LSE+ GVVRV+P+ L+ L TTRSWDFLGLS S SPSN+L  S++GDG+IIGV+DTGIWP
Sbjct: 92  KLSELPGVVRVIPNSLHKLQTTRSWDFLGLS-SQSPSNILHKSSMGDGVIIGVLDTGIWP 151

Query: 137 ETEAFDDQGLGPVPVRWKGECQSGEQFKATRDCNRKIIGAKWYVDGFIAEFGE-FNRSAV 196
           E+E+F+++GLGPVP  WKG C+SG++F AT DCNRKIIGA+W++DG +AE+G+  NRS  
Sbjct: 152 ESESFNEKGLGPVPSHWKGVCESGDRFNATIDCNRKIIGARWFIDGLLAEYGKPLNRST- 211

Query: 197 KEFMSPRDSNGHGTQTASTAAGSFVKNVSYRGLGGGTARGGAPAARLAVYKACWSLPGGG 256
            EF+SPRD++GHGT T+STAAGSFV NVSY+GLG GT +GGAP ARLA+YK CW +   G
Sbjct: 212 -EFLSPRDAHGHGTHTSSTAAGSFVTNVSYKGLGHGTIKGGAPNARLAIYKVCWKVL--G 271

Query: 257 GQCSAADVLKGMDDAVHDGVDVLSLSLGKSAPLFAEVDEENVVAIGSFRANMRNISVVCS 316
           GQCS+AD+LK  D+A+HDGVDVLSLS+G S PLF+EVDE + +A GSF A  R I+VVC+
Sbjct: 272 GQCSSADILKAFDEAIHDGVDVLSLSIGFSIPLFSEVDEHDGIATGSFHAVARGITVVCA 331

Query: 317 AGNDGPSAETVINTSPWILSVAATSIDRTFLGSITLGNNNTFLSQTLYTRKEIGYSGLVY 376
           A NDGPSAETV NT+PWI++VAA+++DR+F  SITLGNN TFL   ++T  EIG++ L+Y
Sbjct: 332 AANDGPSAETVQNTAPWIITVAASTMDRSFPTSITLGNNKTFLGLAMFTGPEIGFASLIY 391

Query: 377 PRS-GLNQIIAGLCDSITLVNGSNWMSGKVVLCFSTMVGMNPIAITRAVEAVKVGNGLGL 436
           P S GL+   AG+C S++L      ++GKVVLCF+T+      AIT A  AVK   G+GL
Sbjct: 392 PESKGLDPTAAGVCQSLSL--SKTMVAGKVVLCFTTV--SRRTAITSASAAVKEAGGVGL 451

Query: 437 IVARHPDDIWFTCDNDFPCFQVNFEVGTKIFYYIRATRSPMVKLSPSRTITGNPISAHIA 496
           IVA++P D+ + C+ DFPC +V++E+GT+I +YIR+TRSP+VKL P +T  G P+SA +A
Sbjct: 452 IVAKNPSDVLYPCNEDFPCIEVDYEIGTRILFYIRSTRSPLVKLRPPKTFIGKPLSAKVA 511

Query: 497 YFSSRGPNSVAPAILKPDIGAPGVAILVATSPFDITNDGGFTIQTGTSIATPHISAIVAL 556
           YFSSRGPNS+ PAILKPDI APGV IL ATSP D   +GG+ + +GTS++TPH++ IVAL
Sbjct: 512 YFSSRGPNSITPAILKPDIAAPGVNILAATSPLDALGEGGYVMHSGTSMSTPHVAGIVAL 571

Query: 557 LKSLHPTWSPAAIKSAIITTAWSRHPSGVPILAEGSPHKLGNPFDYGGGIVNPNAAANPG 616
           LK++HP WSPAAIKSA++TTAW   PSG+PI AEGSP KL NPFD+GGGI+NPN AA+PG
Sbjct: 572 LKAMHPNWSPAAIKSALVTTAWRNGPSGLPIFAEGSPQKLANPFDFGGGIMNPNGAADPG 631

Query: 617 LIYDMSTSDYISYFCSMGYKNSAISFLTEQKTECPSKPQISLLDLNLPSITVPALTNSTT 676
           L+YD+  + Y+ Y C+ GY NSAIS L  Q T+CP K + S+LD+NLPSIT+P+L N  T
Sbjct: 632 LVYDIGKAGYMQYLCARGYNNSAISRLVGQNTKCPIK-KPSILDMNLPSITIPSLKNPIT 691

Query: 677 ITRTVTNVGNLTSIYKALIHPPMGINVTVDPLVLLFNATVTNLSFKVMISTLLEMDYGFS 736
           I R+VTNVG   SIY+A I PP+G  V+V+P  L+FN+TV  L F + IS +  M+ G+ 
Sbjct: 692 IKRSVTNVGAPESIYRATIEPPIGTIVSVNPNALVFNSTVRKLDFTITISAIHRMNTGYY 751

Query: 737 FGAIIWSDGVHLVTTPLSVRTQIL 759
           FG++ W+DGVH+V  PLSVRT+ L
Sbjct: 752 FGSLSWADGVHVVRIPLSVRTEFL 765

BLAST of CcUC07G135880.1 vs. TAIR 10
Match: AT1G32940.1 (Subtilase family protein )

HSP 1 Score: 808.1 bits (2086), Expect = 5.9e-234
Identity = 406/747 (54.35%), Postives = 544/747 (72.82%), Query Frame = 0

Query: 17  LHIVHLEHNQHDPNSSIVEIHHNFLSTLLGSKKKASKSMVYSYTHAFSGFAAKLTKSQAY 76
           +HIV+L   QHD    + E HH  LS+LLGSK  A +SMVYSY H FSGFAAKLT+SQA 
Sbjct: 29  VHIVYLGEKQHDDPEFVSESHHQMLSSLLGSKVDAHESMVYSYRHGFSGFAAKLTESQAK 88

Query: 77  QLSEMRGVVRVLPSVLYDLHTTRSWDFLGLSPSPSPSNLLQMSNLGDGIIIGVVDTGIWP 136
           +L++   VV V+    Y+L TTR+WD+LGLS + +P+NLL  +N+GD +IIG +DTG+WP
Sbjct: 89  KLADSPEVVHVMADSFYELATTRTWDYLGLSVA-NPNNLLNDTNMGDQVIIGFIDTGVWP 148

Query: 137 ETEAFDDQGLGPVPVRWKGECQSGEQFKATRDCNRKIIGAKWYVDGFIAEFGEFNRSAVK 196
           E+E+F+D G+GP+P  WKG C+SGE+F +T +CNRK+IGAK++++GF+AE   FN +  +
Sbjct: 149 ESESFNDNGVGPIPSHWKGGCESGEKFIST-NCNRKLIGAKYFINGFLAENEGFNTTESR 208

Query: 197 EFMSPRDSNGHGTQTASTAAGSFVKNVSYRGLGGGTARGGAPAARLAVYKACWSLPG-GG 256
           +++S RD  GHGT TAS A GSFV N+SY+GL GG  RGGAP AR+A+YKACW +   G 
Sbjct: 209 DYISARDFIGHGTHTASIAGGSFVPNISYKGLAGGNLRGGAPRARIAIYKACWYVDQLGA 268

Query: 257 GQCSAADVLKGMDDAVHDGVDVLSLSLGKSAPLFAEVDEENVVAIGSFRANMRNISVVCS 316
             CS++D+LK MD+++HDGVDVLSLSLG   PL+ E D  + +A G+F A  + I VVC+
Sbjct: 269 VACSSSDILKAMDESMHDGVDVLSLSLGAQIPLYPETDLRDRIATGAFHAVAKGIIVVCA 328

Query: 317 AGNDGPSAETVINTSPWILSVAATSIDRTFLGSITLGNNNTFLSQTLYTRKEIGYSGLVY 376
            GN GP+A+TV+NT+PWI++VAAT++DR+F   ITLGN    L Q LYT +E+G++ LVY
Sbjct: 329 GGNSGPAAQTVLNTAPWIITVAATTLDRSFPTPITLGNRKVILGQALYTGQELGFTSLVY 388

Query: 377 PRSG--LNQIIAGLCDSITLVNGSNWMSGKVVLCFSTMVGMNPIAITRAVEAVKVGNGLG 436
           P +    N+  +G+C+ + L N +  M+GKVVLCF+T       A++RA   VK   GLG
Sbjct: 389 PENAGFTNETFSGVCERLNL-NPNRTMAGKVVLCFTTNTLFT--AVSRAASYVKAAGGLG 448

Query: 437 LIVARHPDDIWFTCDNDFPCFQVNFEVGTKIFYYIRATRSPMVKLSPSRTITGNPISAHI 496
           +I+AR+P      C +DFPC  +++E+GT +  YIR+TRSP+VK+ PSRT+ G P+   +
Sbjct: 449 VIIARNPGYNLTPCRDDFPCVAIDYELGTDVLLYIRSTRSPVVKIQPSRTLVGQPVGTKV 508

Query: 497 AYFSSRGPNSVAPAILKPDIGAPGVAILVATSPFDITNDGGFTIQTGTSIATPHISAIVA 556
           A FSSRGPNS++PAILKPDIGAPGV+IL ATSP   ++ GGF I  GTS+A P ++ +VA
Sbjct: 509 ATFSSRGPNSISPAILKPDIGAPGVSILAATSPDSNSSVGGFDILAGTSMAAPVVAGVVA 568

Query: 557 LLKSLHPTWSPAAIKSAIITTAWSRHPSGVPILAEGSPHKLGNPFDYGGGIVNPNAAANP 616
           LLK+LHP WSPAA +SAI+TTAW   P G  I AEGS  K+ +PFDYGGGIVNP  AA+P
Sbjct: 569 LLKALHPNWSPAAFRSAIVTTAWRTDPFGEQIFAEGSSRKVADPFDYGGGIVNPEKAADP 628

Query: 617 GLIYDMSTSDYISYFCSMGYKNSAISFLTEQKTECPSKPQISLLDLNLPSITVPALTNST 676
           GLIYDM   DYI Y CS GY +S+I+ L    T C S P+ S+LD+NLPSIT+P L +  
Sbjct: 629 GLIYDMGPRDYILYLCSAGYNDSSITQLVGNVTVC-STPKTSVLDVNLPSITIPDLKDEV 688

Query: 677 TITRTVTNVGNLTSIYKALIHPPMGINVTVDPLVLLFNATVTNLSFKVMISTLLEMDYGF 736
           T+TRTVTNVG + S+YK ++ PP+GI V V P  L+FN+   N+SF V +ST  +++ GF
Sbjct: 689 TLTRTVTNVGTVDSVYKVVVEPPLGIQVVVAPETLVFNSKTKNVSFTVRVSTTHKINTGF 748

Query: 737 SFGAIIWSDGVHLVTTPLSVRTQILHS 761
            FG +IW+D +H VT P+SVRTQIL +
Sbjct: 749 YFGNLIWTDSMHNVTIPVSVRTQILQN 769

BLAST of CcUC07G135880.1 vs. TAIR 10
Match: AT4G10540.1 (Subtilase family protein )

HSP 1 Score: 801.6 bits (2069), Expect = 5.5e-232
Identity = 407/747 (54.48%), Postives = 536/747 (71.75%), Query Frame = 0

Query: 17  LHIVHLEHNQHDPNSSIVEIHHNFLSTLLGSKKKASKSMVYSYTHAFSGFAAKLTKSQAY 76
           +HIV+L   QHD    + E HH  L +LLGSK+ A  SMV+SY H FSGFAAKLTKSQA 
Sbjct: 30  VHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDAHSSMVHSYRHGFSGFAAKLTKSQAK 89

Query: 77  QLSEMRGVVRVLPSVLYDLHTTRSWDFLGLSPSPSPSNLLQMSNLGDGIIIGVVDTGIWP 136
           +L+++  VV V P   Y L TTR+WD+LGLS + +P NLL  +N+G+ +IIG+VD+G+WP
Sbjct: 90  KLADLPEVVHVTPDSFYQLDTTRTWDYLGLSVA-NPKNLLNDTNMGEEVIIGIVDSGVWP 149

Query: 137 ETEAFDDQGLGPVPVRWKGECQSGEQFKATRDCNRKIIGAKWYVDGFIAEFGEFNRSAVK 196
           E+E F+D G+GPVP  WKG C SGE F +++ CN+K+IGAK++++GF+A    FN +   
Sbjct: 150 ESEVFNDNGIGPVPSHWKGGCVSGENFTSSQ-CNKKLIGAKYFINGFLATHESFNSTESL 209

Query: 197 EFMSPRDSNGHGTQTASTAAGSFVKNVSYRGLGGGTARGGAPAARLAVYKACWSLPGGG- 256
           +F+SPRD +GHGT  A+ A GS+V ++SY+GL GGT RGGAP AR+A+YKACW L     
Sbjct: 210 DFISPRDRSGHGTHVATIAGGSYVPSISYKGLAGGTVRGGAPRARIAMYKACWYLDRFDI 269

Query: 257 GQCSAADVLKGMDDAVHDGVDVLSLSLGKSAPLFAEVDEENVVAIGSFRANMRNISVVCS 316
             CS+AD+LK MD+A+HDGVDVLSLS+G   P F E D   V+A G+F A ++ I+VVCS
Sbjct: 270 NTCSSADILKAMDEAMHDGVDVLSLSIGYRFPYFPETDVRAVIATGAFHAVLKGITVVCS 329

Query: 317 AGNDGPSAETVINTSPWILSVAATSIDRTFLGSITLGNNNTFLSQTLYTRKEIGYSGLVY 376
            GN GP+A+TV NT+PWIL+VAAT++DR+F   ITLGNN   L Q +YT  E+G++ LVY
Sbjct: 330 GGNSGPAAQTVGNTAPWILTVAATTLDRSFPTPITLGNNKLILGQAMYTGPELGFTSLVY 389

Query: 377 PRS--GLNQIIAGLCDSITLVNGSNWMSGKVVLCFSTMVGMNPIAITRAVEAVKVGNGLG 436
           P +    N+  +G C+ +   N ++ M+GKVVLCF+T      I ++ AV  VK   GLG
Sbjct: 390 PENPGNSNESFSGDCE-LLFFNSNHTMAGKVVLCFTT--STRYITVSSAVSYVKEAGGLG 449

Query: 437 LIVARHPDDIWFTCDNDFPCFQVNFEVGTKIFYYIRATRSPMVKLSPSRTITGNPISAHI 496
           +IVAR+P D    C++DFPC  V++E+GT I  YIR+T  P+VK+ PS+T+ G P+   +
Sbjct: 450 VIVARNPGDNLSPCEDDFPCVAVDYELGTDILLYIRSTGLPVVKIQPSKTLVGQPVGTKV 509

Query: 497 AYFSSRGPNSVAPAILKPDIGAPGVAILVATSPFDITNDGGFTIQTGTSIATPHISAIVA 556
           A FSSRGPNS+ PAILKPDI APGV+IL AT+     ND GF   +GTS+A P IS +VA
Sbjct: 510 ADFSSRGPNSIEPAILKPDIAAPGVSILAATTTNKTFNDRGFIFLSGTSMAAPTISGVVA 569

Query: 557 LLKSLHPTWSPAAIKSAIITTAWSRHPSGVPILAEGSPHKLGNPFDYGGGIVNPNAAANP 616
           LLK+LH  WSPAAI+SAI+TTAW   P G  I AEGSP KL +PFDYGGG+VNP  AA P
Sbjct: 570 LLKALHRDWSPAAIRSAIVTTAWRTDPFGEQIFAEGSPRKLADPFDYGGGLVNPEKAAKP 629

Query: 617 GLIYDMSTSDYISYFCSMGYKNSAISFLTEQKTECPSKPQISLLDLNLPSITVPALTNST 676
           GL+YD+   DY+ Y CS+GY  ++IS L  + T C S P+ S+LD NLPSIT+P L +  
Sbjct: 630 GLVYDLGLEDYVLYMCSVGYNETSISQLVGKGTVC-SNPKPSVLDFNLPSITIPNLKDEV 689

Query: 677 TITRTVTNVGNLTSIYKALIHPPMGINVTVDPLVLLFNATVTNLSFKVMISTLLEMDYGF 736
           T+TRT+TNVG L S+YK +I PP+GI VTV P  LLFN+T   +SFKV +ST  +++ G+
Sbjct: 690 TLTRTLTNVGQLESVYKVVIEPPIGIQVTVTPETLLFNSTTKRVSFKVKVSTTHKINTGY 749

Query: 737 SFGAIIWSDGVHLVTTPLSVRTQILHS 761
            FG++ WSD +H VT PLSVRTQIL +
Sbjct: 750 FFGSLTWSDSLHNVTIPLSVRTQILQN 770

BLAST of CcUC07G135880.1 vs. TAIR 10
Match: AT1G32960.1 (Subtilase family protein )

HSP 1 Score: 795.0 bits (2052), Expect = 5.2e-230
Identity = 406/753 (53.92%), Postives = 540/753 (71.71%), Query Frame = 0

Query: 11  TQFDQLLHIVHLEHNQHDPNSSIVEIHHNFLSTLLGSKKKASKSMVYSYTHAFSGFAAKL 70
           ++ +  +HIV+L   +H     + E HH  L++LLGSKK A  SMVYSY H FSGFAAKL
Sbjct: 26  SETESKVHIVYLGEKKHHDPEFVTESHHQMLASLLGSKKDADDSMVYSYRHGFSGFAAKL 85

Query: 71  TKSQAYQLSEMRGVVRVLPSVLYDLHTTRSWDFLGLSPSPSPSNLLQMSNLGDGIIIGVV 130
           TKSQA +++++  VV V+P   ++L TTR+W++LGLS S +P NLL  +N+GD +IIGV+
Sbjct: 86  TKSQAKKIADLPEVVHVIPDGFHELATTRTWEYLGLS-SANPKNLLNDTNMGDQVIIGVI 145

Query: 131 DTGIWPETEAFDDQGLGPVPVRWKGECQSGEQFKATRDCNRKIIGAKWYVDGFIAEFGEF 190
           DTG+WPE+E+F+D G+GP+P +WKG C+SGE F++T DCNRK+IGAK++++GF+AE   F
Sbjct: 146 DTGVWPESESFNDNGVGPIPRKWKGGCESGENFRST-DCNRKLIGAKYFINGFLAENKGF 205

Query: 191 NRSAVKEFMSPRDSNGHGTQTASTAAGSFVKNVSYRGLGGGTARGGAPAARLAVYKACWS 250
           N +  ++++S RD +GHGT  AS A GSFV NVSY+GL GGT RGGAP AR+A+YKACW 
Sbjct: 206 NTTESRDYISARDFDGHGTHVASIAGGSFVPNVSYKGLAGGTLRGGAPRARIAMYKACWF 265

Query: 251 LPG-GGGQCSAADVLKGMDDAVHDGVDVLSLSLGKSAPLFAEVDEENVVAIGSFRANMRN 310
                G  CS +D++K +D+A+HDGVDVLS+SL    PL +E D  +  A G F A  + 
Sbjct: 266 HEELKGVTCSDSDIMKAIDEAIHDGVDVLSISLVGQIPLNSETDIRDEFATGLFHAVAKG 325

Query: 311 ISVVCSAGNDGPSAETVINTSPWILSVAATSIDRTFLGSITLGNNNTFLSQTLYTRKEIG 370
           I VVC+ GNDGP+A+TV+N +PWIL+VAAT++DR+F   ITLGNN   L Q  YT  E+G
Sbjct: 326 IVVVCAGGNDGPAAQTVVNIAPWILTVAATTLDRSFPTPITLGNNKVILGQATYTGPELG 385

Query: 371 YSGLVYPRSGL--NQIIAGLCDSITLVNGSNWMSGKVVLCFSTMVGMNPIAITRAVEAVK 430
            + LVYP +    N+  +G+C+S+ L N +  M+ KVVLCF+        AI+RA   VK
Sbjct: 386 LTSLVYPENARNNNETFSGVCESLNL-NPNYTMAMKVVLCFT--ASRTNAAISRAASFVK 445

Query: 431 VGNGLGLIVARHPDDIWFTCDNDFPCFQVNFEVGTKIFYYIRATRSPMVKLSPSRTITGN 490
              GLGLI++R+P      C++DFPC  V++E+GT I  YIR+TRSP+VK+  SRT++G 
Sbjct: 446 AAGGLGLIISRNPVYTLSPCNDDFPCVAVDYELGTDILSYIRSTRSPVVKIQRSRTLSGQ 505

Query: 491 PISAHIAYFSSRGPNSVAPAILKPDIGAPGVAILVATSPFDITNDGGFTIQTGTSIATPH 550
           P+   +  FSSRGPNS++PAILKPDI APGV IL ATSP D  N GGF + +GTS+ATP 
Sbjct: 506 PVGTKVVNFSSRGPNSMSPAILKPDIAAPGVRILAATSPNDTLNVGGFAMLSGTSMATPV 565

Query: 551 ISAIVALLKSLHPTWSPAAIKSAIITTAWSRHPSGVPILAEGSPHKLGNPFDYGGGIVNP 610
           IS ++ALLK+LHP WSPAA +SAI+TTAW   P G  I AEGS  K+ +PFDYGGGIVNP
Sbjct: 566 ISGVIALLKALHPEWSPAAFRSAIVTTAWRTDPFGEQIFAEGSSRKVSDPFDYGGGIVNP 625

Query: 611 NAAANPGLIYDMSTSDYISYFCSMGYKNSAISFLTEQKTECPSKPQISLLDLNLPSITVP 670
             AA PGLIYDM   DYI Y CS GY +S+IS L  Q T C S P+ S+LD+NLPSIT+P
Sbjct: 626 EKAAEPGLIYDMGPQDYILYLCSAGYNDSSISQLVGQITVC-SNPKPSVLDVNLPSITIP 685

Query: 671 ALTNSTTITRTVTNVGNLTSIYKALIHPPMGINVTVDPLVLLFNATVTNLSFKVMISTLL 730
            L +  T+TRTVTNVG + S+YK  + PP+G+ V V P  L+FN+   ++SF V +ST  
Sbjct: 686 NLKDEVTLTRTVTNVGLVDSVYKVSVEPPLGVRVVVTPETLVFNSKTISVSFTVRVSTTH 745

Query: 731 EMDYGFSFGAIIWSDGVHLVTTPLSVRTQILHS 761
           +++ G+ FG++ W+D VH V  PLSVRTQIL +
Sbjct: 746 KINTGYYFGSLTWTDSVHNVVIPLSVRTQILQN 772

BLAST of CcUC07G135880.1 vs. TAIR 10
Match: AT4G10520.1 (Subtilase family protein )

HSP 1 Score: 790.0 bits (2039), Expect = 1.7e-228
Identity = 407/749 (54.34%), Postives = 532/749 (71.03%), Query Frame = 0

Query: 10  FTQFDQLLHIVHLEHNQHDPNSSIVEIHHNFLSTLLGSKKKASKSMVYSYTHAFSGFAAK 69
           F   +  +++V+L   +HD   S+ E HH  L +LLGSK+    S+VYSY H FSGFAAK
Sbjct: 22  FVVAESKVYVVYLGEKEHDNPESVTESHHQMLWSLLGSKEAVLDSIVYSYRHGFSGFAAK 81

Query: 70  LTKSQAYQLSEMRGVVRVLPSVLYDLHTTRSWDFLGLSPSPSPSNLLQMSNLGDGIIIGV 129
           LT+SQA Q+SE+  VV+V+P+ LY++ TTR+WD+LG+SP  S S LLQ +N+G  +I+GV
Sbjct: 82  LTESQAQQISELPEVVQVIPNTLYEMTTTRTWDYLGVSPGNSDS-LLQKANMGYNVIVGV 141

Query: 130 VDTGIWPETEAFDDQGLGPVPVRWKGECQSGEQFKATRDCNRKIIGAKWYVDGFIAEFGE 189
           +D+G+WPE+E F+D+G GP+P RWKG C+SGE F A+  CNRK+IGAK++VDG +AEFG 
Sbjct: 142 IDSGVWPESEMFNDKGFGPIPSRWKGGCESGELFNASIHCNRKLIGAKYFVDGLVAEFGV 201

Query: 190 FNRSAVKEFMSPRDSNGHGTQTASTAAGSFVKNVSYRGLGGGTARGGAPAARLAVYKACW 249
            NR+   E++SPRD  GHGT  AST  GSF+ NVSY GLG GTARGGAP   +AVYKACW
Sbjct: 202 VNRTQNPEYLSPRDFAGHGTHVASTIGGSFLPNVSYVGLGRGTARGGAPGVHIAVYKACW 261

Query: 250 SLPGGGGQCSAADVLKGMDDAVHDGVDVLSLSLGKSAPLFAEVDEENVVAIGSFRANMRN 309
           S     G CS ADVLK MD+A+HDGVD+LSLSLG S PLF E +     ++G+F A  + 
Sbjct: 262 S-----GYCSGADVLKAMDEAIHDGVDILSLSLGPSVPLFPETEH---TSVGAFHAVAKG 321

Query: 310 ISVVCSAGNDGPSAETVINTSPWILSVAATSIDRTFLGSITLGNNNTFLSQTLYTRKEIG 369
           I VV +AGN GP+A+T+ N +PW+L+VAAT+ DR+F  +ITLGNN T L Q +Y   E+G
Sbjct: 322 IPVVIAAGNAGPTAQTISNVAPWVLTVAATTQDRSFPTAITLGNNITILGQAIYGGPELG 381

Query: 370 YSGLVYPRSGLNQIIAGLCDSITLVNGSNWMSGKVVLCFSTMVGMNPIAITRAVEAVKVG 429
           + GL YP S L    +G C+ ++  N ++ M GKVVLCF+     N      A+ AV   
Sbjct: 382 FVGLTYPESPL----SGDCEKLS-ANPNSTMEGKVVLCFAASTPSN-----AAIAAVINA 441

Query: 430 NGLGLIVARHPDDIWFTCDNDFPCFQVNFEVGTKIFYYIRATRSPMVKLSPSRTITGNPI 489
            GLGLI+A++P     T    FP   ++FE+GT I +YIR+TRSP+VK+  S+T+ G  +
Sbjct: 442 GGLGLIMAKNPTHS-LTPTRKFPWVSIDFELGTDILFYIRSTRSPIVKIQASKTLFGQSV 501

Query: 490 SAHIAYFSSRGPNSVAPAILKPDIGAPGVAILVATSPFDITNDGGFTIQTGTSIATPHIS 549
           S  +A FSSRGPNSV+PAILKPDI APGV IL A SP    NDGGF + +GTS+ATP +S
Sbjct: 502 STKVATFSSRGPNSVSPAILKPDIAAPGVNILAAISPNSSINDGGFAMMSGTSMATPVVS 561

Query: 550 AIVALLKSLHPTWSPAAIKSAIITTAWSRHPSGVPILAEGSPHKLGNPFDYGGGIVNPNA 609
            +V LLKSLHP WSP+AIKSAI+TTAW   PSG PI A+GS  KL +PFDYGGG++NP  
Sbjct: 562 GVVVLLKSLHPDWSPSAIKSAIVTTAWRTDPSGEPIFADGSSRKLADPFDYGGGLINPEK 621

Query: 610 AANPGLIYDMSTSDYISYFCSMGYKNSAISFLTEQKTECPSKPQISLLDLNLPSITVPAL 669
           A  PGLIYDM+T DY+ Y CS+ Y + +IS +  + T CP+ P+ S+LDLNLPSIT+P L
Sbjct: 622 AVKPGLIYDMTTDDYVMYMCSVDYSDISISRVLGKITVCPN-PKPSVLDLNLPSITIPNL 681

Query: 670 TNSTTITRTVTNVGNLTSIYKALIHPPMGINVTVDPLVLLFNATVTNLSFKVMISTLLEM 729
               T+TRTVTNVG + S+YK +I PP GINV V P  L+F+ T T  SF V +ST  ++
Sbjct: 682 RGEVTLTRTVTNVGPVNSVYKVVIDPPTGINVAVTPAELVFDYTTTKRSFTVRVSTTHKV 741

Query: 730 DYGFSFGAIIWSDGVHLVTTPLSVRTQIL 759
           + G+ FG++ W+D +H V  P+SVRTQIL
Sbjct: 742 NTGYYFGSLTWTDNMHNVAIPVSVRTQIL 749

BLAST of CcUC07G135880.1 vs. TAIR 10
Match: AT1G32950.1 (Subtilase family protein )

HSP 1 Score: 786.2 bits (2029), Expect = 2.4e-227
Identity = 401/747 (53.68%), Postives = 533/747 (71.35%), Query Frame = 0

Query: 17  LHIVHLEHNQHDPNSSIVEIHHNFLSTLLGSKKKASKSMVYSYTHAFSGFAAKLTKSQAY 76
           +HIV+L   QHD    + E HH  LS+LLGSK  A +SMVYSY H FSGFAAKLTKSQA 
Sbjct: 29  VHIVYLGEKQHDDPKFVTESHHQMLSSLLGSKDDAHESMVYSYRHGFSGFAAKLTKSQAK 88

Query: 77  QLSEMRGVVRVLPSVLYDLHTTRSWDFLGLSPSPSPSNLLQMSNLGDGIIIGVVDTGIWP 136
           ++++   V+ V+P   Y+L TTR WD+LG S   S  NL+  +N+GD  IIGV+DTG+WP
Sbjct: 89  KIADSPEVIHVIPDSYYELATTRIWDYLGPSADNS-KNLVSDTNMGDQTIIGVIDTGVWP 148

Query: 137 ETEAFDDQGLGPVPVRWKGECQSGEQFKATRDCNRKIIGAKWYVDGFIAEFGEFNRSAVK 196
           E+E+F+D G+GPVP  WKG C+ GE F +T +CNRK+IGAK++++GF+AE  +FN +   
Sbjct: 149 ESESFNDYGVGPVPSHWKGGCEPGENFIST-NCNRKLIGAKYFINGFLAE-NQFNATESP 208

Query: 197 EFMSPRDSNGHGTQTASTAAGSFVKNVSYRGLGGGTARGGAPAARLAVYKACWSLPG-GG 256
           +++S RD +GHGT  AS A GSFV NVSY+GLG GT RGGAP AR+A+YKACW +    G
Sbjct: 209 DYISARDFDGHGTHVASIAGGSFVPNVSYKGLGRGTLRGGAPRARIAMYKACWYINELDG 268

Query: 257 GQCSAADVLKGMDDAVHDGVDVLSLSLGKSAPLFAEVDEENVVAIGSFRANMRNISVVCS 316
             CS +D++K +D+A+HDGVDVLS+SLG   PL +E D  + +A G+F A  + I VVC+
Sbjct: 269 VTCSFSDIMKAIDEAIHDGVDVLSISLGGRVPLNSETDLRDGIATGAFHAVAKGIVVVCA 328

Query: 317 AGNDGPSAETVINTSPWILSVAATSIDRTFLGSITLGNNNTFLSQTLYTRKEIGYSGLVY 376
            GN GPS++TV+NT+PWIL+VAAT++DR+F   I LGNN   L Q +Y   E+G++ LVY
Sbjct: 329 GGNAGPSSQTVVNTAPWILTVAATTLDRSFATPIILGNNQVILGQAMYIGPELGFTSLVY 388

Query: 377 PRSGLNQI--IAGLCDSITLVNGSNWMSGKVVLCFSTMVGMNPIAITRAVEAVKVGNGLG 436
           P    N I   +G+C+S+ L N +  M+GKVVLCF+T    +   ++ A   VK   GLG
Sbjct: 389 PEDPGNSIDTFSGVCESLNL-NSNRTMAGKVVLCFTT--ARDFTVVSTAASIVKAAGGLG 448

Query: 437 LIVARHPDDIWFTCDNDFPCFQVNFEVGTKIFYYIRATRSPMVKLSPSRTITGNPISAHI 496
           LI+AR+P      C +DFPC  ++ E+GT I +YIR T SP+VK+ PSRT+ G P+   +
Sbjct: 449 LIIARNPGYNLAPCSDDFPCVAIDNELGTDILFYIRYTGSPVVKIQPSRTLVGEPVGTKV 508

Query: 497 AYFSSRGPNSVAPAILKPDIGAPGVAILVATSPFDITNDGGFTIQTGTSIATPHISAIVA 556
           A FSSRGPNS++PAILKPDI APGV+IL ATSP D  N GGF +++GTS+A P IS ++A
Sbjct: 509 ATFSSRGPNSISPAILKPDIAAPGVSILAATSPNDTLNAGGFVMRSGTSMAAPVISGVIA 568

Query: 557 LLKSLHPTWSPAAIKSAIITTAWSRHPSGVPILAEGSPHKLGNPFDYGGGIVNPNAAANP 616
           LLKSLHP WSPAA +SAI+TTAW   P G  I AE S  K+ +PFDYGGG+VNP  AA P
Sbjct: 569 LLKSLHPDWSPAAFRSAIVTTAWRTDPFGEQIAAESSSLKVPDPFDYGGGLVNPEKAAEP 628

Query: 617 GLIYDMSTSDYISYFCSMGYKNSAISFLTEQKTECPSKPQISLLDLNLPSITVPALTNST 676
           GLI DM + DY+ Y CS GY +S+IS L  + T C S P+ S+LD+NLPSIT+P L +  
Sbjct: 629 GLILDMDSQDYVLYLCSAGYNDSSISRLVGKVTVC-SNPKPSVLDINLPSITIPNLKDEV 688

Query: 677 TITRTVTNVGNLTSIYKALIHPPMGINVTVDPLVLLFNATVTNLSFKVMISTLLEMDYGF 736
           T+TRTVTNVG + S+YK L+ PP+GI V V P  L+FN+   ++SF V++ST  +++ GF
Sbjct: 689 TLTRTVTNVGPVDSVYKVLVEPPLGIQVVVTPETLVFNSKTKSVSFTVIVSTTHKINTGF 748

Query: 737 SFGAIIWSDGVHLVTTPLSVRTQILHS 761
            FG++ W+D +H V  P+SVRTQIL +
Sbjct: 749 YFGSLTWTDSIHNVVIPVSVRTQILQN 768

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022136960.10.0e+0077.69subtilisin-like protease SBT3.5 isoform X2 [Momordica charantia][more]
XP_022136958.10.0e+0076.66subtilisin-like protease SBT3.5 isoform X1 [Momordica charantia][more]
XP_021679638.12.2e-26261.29subtilisin-like protease SBT3.3 [Hevea brasiliensis][more]
XP_030951705.17.7e-26060.72subtilisin-like protease SBT3.5 [Quercus lobata][more]
PQP94706.12.9e-25960.35subtilisin-like protease SBT3.4 [Prunus yedoensis var. nudiflora][more]
Match NameE-valueIdentityDescription
Q9MAP78.3e-23354.35Subtilisin-like protease SBT3.5 OS=Arabidopsis thaliana OX=3702 GN=SBT3.5 PE=1 S... [more]
Q9SZY37.8e-23154.48Subtilisin-like protease SBT3.8 OS=Arabidopsis thaliana OX=3702 GN=SBT3.8 PE=3 S... [more]
Q9MAP57.3e-22953.92Subtilisin-like protease SBT3.3 OS=Arabidopsis thaliana OX=3702 GN=SBT3.3 PE=2 S... [more]
Q9ZSB02.3e-22754.34Subtilisin-like protease SBT3.9 OS=Arabidopsis thaliana OX=3702 GN=SBT3.9 PE=3 S... [more]
F4HPF13.4e-22653.68Subtilisin-like protease SBT3.4 OS=Arabidopsis thaliana OX=3702 GN=SBT3.4 PE=3 S... [more]
Match NameE-valueIdentityDescription
A0A6J1C5D10.0e+0077.69subtilisin-like protease SBT3.5 isoform X2 OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A6J1C8Y60.0e+0076.66subtilisin-like protease SBT3.5 isoform X1 OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A7N2KV163.7e-26060.72Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1[more]
A0A314XQK51.4e-25960.35Subtilisin-like protease SBT3.4 OS=Prunus yedoensis var. nudiflora OX=2094558 GN... [more]
A0A314UH942.4e-25960.22Subtilisin-like protease SBT3.4 OS=Prunus yedoensis var. nudiflora OX=2094558 GN... [more]
Match NameE-valueIdentityDescription
AT1G32940.15.9e-23454.35Subtilase family protein [more]
AT4G10540.15.5e-23254.48Subtilase family protein [more]
AT1G32960.15.2e-23053.92Subtilase family protein [more]
AT4G10520.11.7e-22854.34Subtilase family protein [more]
AT1G32950.12.4e-22753.68Subtilase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (PI 537277) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 539..555
score: 48.44
coord: 203..216
score: 50.03
coord: 122..141
score: 33.19
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 18..96
e-value: 3.8E-14
score: 53.1
NoneNo IPR availableGENE3D2.60.40.2310coord: 628..756
e-value: 5.1E-33
score: 115.6
NoneNo IPR availableGENE3D3.50.30.30coord: 342..485
e-value: 3.1E-154
score: 516.2
NoneNo IPR availablePANTHERPTHR43806PEPTIDASE S8coord: 14..760
NoneNo IPR availablePANTHERPTHR43806:SF50SUBTILISIN-LIKE PROTEASE SBT3.1-RELATEDcoord: 14..760
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 100..611
score: 27.298273
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 348..478
e-value: 9.22908E-19
score: 80.9207
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3D3.30.70.80Peptidase S8 propeptide/proteinase inhibitor I9coord: 10..96
e-value: 5.8E-23
score: 83.1
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 658..753
e-value: 3.4E-22
score: 78.5
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 124..624
e-value: 3.1E-154
score: 516.2
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 23..680
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 122..575
e-value: 1.0E-44
score: 152.9
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 540..550
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 93..576
e-value: 2.29217E-116
score: 351.515

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CcUC07G135880CcUC07G135880gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
CcUC07G135880.1-exonCcUC07G135880.1-exon-CicolChr07:19012101..19012240exon
CcUC07G135880.1-exonCcUC07G135880.1-exon-CicolChr07:19012313..19012410exon
CcUC07G135880.1-exonCcUC07G135880.1-exon-CicolChr07:19012497..19012655exon
CcUC07G135880.1-exonCcUC07G135880.1-exon-CicolChr07:19012729..19013405exon
CcUC07G135880.1-exonCcUC07G135880.1-exon-CicolChr07:19013509..19013591exon
CcUC07G135880.1-exonCcUC07G135880.1-exon-CicolChr07:19013733..19013990exon
CcUC07G135880.1-exonCcUC07G135880.1-exon-CicolChr07:19014273..19014387exon
CcUC07G135880.1-exonCcUC07G135880.1-exon-CicolChr07:19014464..19014656exon
CcUC07G135880.1-exonCcUC07G135880.1-exon-CicolChr07:19016184..19016749exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CcUC07G135880.1-cdsCcUC07G135880.1-cds-CicolChr07:19012101..19012240CDS
CcUC07G135880.1-cdsCcUC07G135880.1-cds-CicolChr07:19012313..19012410CDS
CcUC07G135880.1-cdsCcUC07G135880.1-cds-CicolChr07:19012497..19012655CDS
CcUC07G135880.1-cdsCcUC07G135880.1-cds-CicolChr07:19012729..19013405CDS
CcUC07G135880.1-cdsCcUC07G135880.1-cds-CicolChr07:19013509..19013591CDS
CcUC07G135880.1-cdsCcUC07G135880.1-cds-CicolChr07:19013733..19013990CDS
CcUC07G135880.1-cdsCcUC07G135880.1-cds-CicolChr07:19014273..19014387CDS
CcUC07G135880.1-cdsCcUC07G135880.1-cds-CicolChr07:19014464..19014656CDS
CcUC07G135880.1-cdsCcUC07G135880.1-cds-CicolChr07:19016184..19016749CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CcUC07G135880.1CcUC07G135880.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity