Carg28230-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg28230-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Histone H2B
Location	Carg_Chr12: 6875620 .. 6876045 (-)
Sequence length	426
RNA-Seq Expression	Carg28230-RA
Synteny	Carg28230-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGCCGAAGGCTGAGAAGAAGCCCGCCGAGAAGAAGCCGGCAGAGGAGAAGAAGGCCGAGAAGGCTCCGGCGGAGAAGAAGCCGAGAGCAGAGAAGAAGCTTCCCAAAGACGGGGCCGATAAGAAGAGGAAGAAGGCGAAGAAGAGCGTGGAGACTTACAAGATCTACATCTTCAAGGTCCTCAAGCAAGTTCATCCTGACATTGGAATCTCCAGTAAGGCCATGGGGATTATGAACAGTTTCATCAACGATATCTTCGAGAAGCTCGCTCAGGAATCCTCCAAACTCGCTCGCTACAACAAGAAACCGACCATCACCTCTCGGGAGATTCAAACCGCTGTGCGCCTTGTTCTTCCTGGTGAGTTGGCTAAGCATGCCGTCTCTGAGGGCACCAAGGCTGTTACCAAGTTTACTAGCTCTTAG

mRNA sequence

ATGGCGCCGAAGGCTGAGAAGAAGCCCGCCGAGAAGAAGCCGGCAGAGGAGAAGAAGGCCGAGAAGGCTCCGGCGGAGAAGAAGCCGAGAGCAGAGAAGAAGCTTCCCAAAGACGGGGCCGATAAGAAGAGGAAGAAGGCGAAGAAGAGCGTGGAGACTTACAAGATCTACATCTTCAAGGTCCTCAAGCAAGTTCATCCTGACATTGGAATCTCCAGTAAGGCCATGGGGATTATGAACAGTTTCATCAACGATATCTTCGAGAAGCTCGCTCAGGAATCCTCCAAACTCGCTCGCTACAACAAGAAACCGACCATCACCTCTCGGGAGATTCAAACCGCTGTGCGCCTTGTTCTTCCTGGTGAGTTGGCTAAGCATGCCGTCTCTGAGGGCACCAAGGCTGTTACCAAGTTTACTAGCTCTTAG

Coding sequence (CDS)

ATGGCGCCGAAGGCTGAGAAGAAGCCCGCCGAGAAGAAGCCGGCAGAGGAGAAGAAGGCCGAGAAGGCTCCGGCGGAGAAGAAGCCGAGAGCAGAGAAGAAGCTTCCCAAAGACGGGGCCGATAAGAAGAGGAAGAAGGCGAAGAAGAGCGTGGAGACTTACAAGATCTACATCTTCAAGGTCCTCAAGCAAGTTCATCCTGACATTGGAATCTCCAGTAAGGCCATGGGGATTATGAACAGTTTCATCAACGATATCTTCGAGAAGCTCGCTCAGGAATCCTCCAAACTCGCTCGCTACAACAAGAAACCGACCATCACCTCTCGGGAGATTCAAACCGCTGTGCGCCTTGTTCTTCCTGGTGAGTTGGCTAAGCATGCCGTCTCTGAGGGCACCAAGGCTGTTACCAAGTTTACTAGCTCTTAG

Protein sequence

MAPKAEKKPAEKKPAEEKKAEKAPAEKKPRAEKKLPKDGADKKRKKAKKSVETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREIQTAVRLVLPGELAKHAVSEGTKAVTKFTSS

Homology

[Close]

BLAST of Carg28230-RA vs. NCBI nr
Match: KAG6586017.1 (hypothetical protein SDJN03_18750, partial [Cucurbita argyrosperma subsp. sororia] >KAG7020793.1 hypothetical protein SDJN02_17481, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 251.1 bits (640), Expect = 5.5e-63
Identity = 141/141 (100.00%), Postives = 141/141 (100.00%), Query Frame = 0


Query: 1   MAPKAEKKPAEKKPAEEKKAEKAPAEKKPRAEKKLPKDGADKKRKKAKKSVETYKIYIFK 60
           MAPKAEKKPAEKKPAEEKKAEKAPAEKKPRAEKKLPKDGADKKRKKAKKSVETYKIYIFK
Sbjct: 1   MAPKAEKKPAEKKPAEEKKAEKAPAEKKPRAEKKLPKDGADKKRKKAKKSVETYKIYIFK 60

Query: 61  VLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREIQTAVRLVLP 120
           VLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREIQTAVRLVLP
Sbjct: 61  VLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREIQTAVRLVLP 120

Query: 121 GELAKHAVSEGTKAVTKFTSS 142
           GELAKHAVSEGTKAVTKFTSS
Sbjct: 121 GELAKHAVSEGTKAVTKFTSS 141

[Close]

BLAST of Carg28230-RA vs. NCBI nr
Match: XP_022937557.1 (histone H2B-like [Cucurbita moschata] >XP_022937558.1 histone H2B-like [Cucurbita moschata] >XP_022969534.1 histone H2B [Cucurbita maxima] >XP_023537508.1 histone H2B [Cucurbita pepo subsp. pepo] >XP_023537509.1 histone H2B [Cucurbita pepo subsp. pepo] >KAG6586018.1 hypothetical protein SDJN03_18751, partial [Cucurbita argyrosperma subsp. sororia] >KAG7020794.1 hypothetical protein SDJN02_17482, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 250.0 bits (637), Expect = 1.2e-62
Identity = 140/141 (99.29%), Postives = 141/141 (100.00%), Query Frame = 0


Query: 1   MAPKAEKKPAEKKPAEEKKAEKAPAEKKPRAEKKLPKDGADKKRKKAKKSVETYKIYIFK 60
           MAPKAEKKPAEKKPAEEKKAEKAPAEKKPRAEKKLPKDGADKK+KKAKKSVETYKIYIFK
Sbjct: 1   MAPKAEKKPAEKKPAEEKKAEKAPAEKKPRAEKKLPKDGADKKKKKAKKSVETYKIYIFK 60

Query: 61  VLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREIQTAVRLVLP 120
           VLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREIQTAVRLVLP
Sbjct: 61  VLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREIQTAVRLVLP 120

Query: 121 GELAKHAVSEGTKAVTKFTSS 142
           GELAKHAVSEGTKAVTKFTSS
Sbjct: 121 GELAKHAVSEGTKAVTKFTSS 141

[Close]

BLAST of Carg28230-RA vs. NCBI nr
Match: XP_022969535.1 (histone H2B-like [Cucurbita maxima])

HSP 1 Score: 243.8 bits (621), Expect = 8.8e-61
Identity = 139/141 (98.58%), Postives = 140/141 (99.29%), Query Frame = 0


Query: 1   MAPKAEKKPAEKKPAEEKKAEKAPAEKKPRAEKKLPKDGADKKRKKAKKSVETYKIYIFK 60
           MAPKAEKKPAEKKPAEEKKA+KAPAEKKPRAEKKLPKDGADKK KKAKKSVETYKIYIFK
Sbjct: 1   MAPKAEKKPAEKKPAEEKKADKAPAEKKPRAEKKLPKDGADKK-KKAKKSVETYKIYIFK 60

Query: 61  VLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREIQTAVRLVLP 120
           VLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREIQTAVRLVLP
Sbjct: 61  VLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREIQTAVRLVLP 120

Query: 121 GELAKHAVSEGTKAVTKFTSS 142
           GELAKHAVSEGTKAVTKFTSS
Sbjct: 121 GELAKHAVSEGTKAVTKFTSS 140

[Close]

BLAST of Carg28230-RA vs. NCBI nr
Match: XP_022964661.1 (histone H2B.7-like [Cucurbita moschata] >XP_022970348.1 histone H2B.7-like [Cucurbita maxima] >XP_023519437.1 histone H2B.7-like [Cucurbita pepo subsp. pepo] >KAG6583589.1 Histone H2B, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019290.1 Histone H2B, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 241.5 bits (615), Expect = 4.4e-60
Identity = 137/142 (96.48%), Postives = 140/142 (98.59%), Query Frame = 0


Query: 1   MAPKAEKKPAEKKP-AEEKKAEKAPAEKKPRAEKKLPKDGADKKRKKAKKSVETYKIYIF 60
           MAPKAEKKPAEKKP AEEKKAEKAPAEKKPRAEKKLPKD ADKK+K+AKKS+ETYKIYIF
Sbjct: 1   MAPKAEKKPAEKKPAAEEKKAEKAPAEKKPRAEKKLPKDAADKKKKRAKKSIETYKIYIF 60

Query: 61  KVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREIQTAVRLVL 120
           KVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREIQTAVRLVL
Sbjct: 61  KVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREIQTAVRLVL 120

Query: 121 PGELAKHAVSEGTKAVTKFTSS 142
           PGELAKHAVSEGTKAVTKFTSS
Sbjct: 121 PGELAKHAVSEGTKAVTKFTSS 142

[Close]

BLAST of Carg28230-RA vs. NCBI nr
Match: XP_030959009.1 (probable histone H2B.1 [Quercus lobata])

HSP 1 Score: 241.5 bits (615), Expect = 4.4e-60
Identity = 134/141 (95.04%), Postives = 140/141 (99.29%), Query Frame = 0


Query: 1   MAPKAEKKPAEKKPAEEKKAEKAPAEKKPRAEKKLPKDGADKKRKKAKKSVETYKIYIFK 60
           MAPKAEKKPAEKKPAEEKKAEKAPAEKKPRAEKKLPKD ++KK+K++KKSVETYKIYIFK
Sbjct: 1   MAPKAEKKPAEKKPAEEKKAEKAPAEKKPRAEKKLPKDSSEKKKKRSKKSVETYKIYIFK 60

Query: 61  VLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREIQTAVRLVLP 120
           VLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESS+LARYNKKPTITSREIQTAVRLVLP
Sbjct: 61  VLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSREIQTAVRLVLP 120

Query: 121 GELAKHAVSEGTKAVTKFTSS 142
           GELAKHAVSEGTKAVTKFTSS
Sbjct: 121 GELAKHAVSEGTKAVTKFTSS 141

[Close]

BLAST of Carg28230-RA vs. ExPASy Swiss-Prot
Match: O22582 (Histone H2B OS=Gossypium hirsutum OX=3635 GN=HIS2B PE=2 SV=3)

HSP 1 Score: 227.3 bits (578), Expect = 1.1e-58
Identity = 132/147 (89.80%), Postives = 138/147 (93.88%), Query Frame = 0


Query: 1   MAPKAEKKPAEKKPAEEKK--AEKAPAEKKPRAEKKLPKDG----ADKKRKKAKKSVETY 60
           MAPKAEKKPAEKKPAEEKK  AEKAPAEKKP+A KKLPK+G     DKK+K+ KKSVETY
Sbjct: 1   MAPKAEKKPAEKKPAEEKKAVAEKAPAEKKPKAGKKLPKEGGAAAGDKKKKRVKKSVETY 60

Query: 61  KIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREIQTA 120
           KIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQE+S+LARYNKKPTITSREIQTA
Sbjct: 61  KIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQEASRLARYNKKPTITSREIQTA 120

Query: 121 VRLVLPGELAKHAVSEGTKAVTKFTSS 142
           VRLVLPGELAKHAVSEGTKAVTKFTSS
Sbjct: 121 VRLVLPGELAKHAVSEGTKAVTKFTSS 147

[Close]

BLAST of Carg28230-RA vs. ExPASy Swiss-Prot
Match: O65821 (Histone H2B.1 OS=Solanum lycopersicum OX=4081 GN=H2B-1 PE=2 SV=4)

HSP 1 Score: 226.5 bits (576), Expect = 1.9e-58
Identity = 129/142 (90.85%), Postives = 136/142 (95.77%), Query Frame = 0


Query: 1   MAPKAEKKPAEKKPAEEKKAEKAPAEKKPRAEKKLPKD-GADKKRKKAKKSVETYKIYIF 60
           MAPKA KKPAEKKP EEKKAE+ PAEKKP+A KKLPKD GADKK+KK+KKSVETYKIYIF
Sbjct: 1   MAPKAGKKPAEKKPVEEKKAEEVPAEKKPKAGKKLPKDAGADKKKKKSKKSVETYKIYIF 60

Query: 61  KVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREIQTAVRLVL 120
           KVLKQVHPDIGISSK+MGIMNSFINDIFEKLAQESS+LAR NKKPTITSREIQTAVRLVL
Sbjct: 61  KVLKQVHPDIGISSKSMGIMNSFINDIFEKLAQESSRLARINKKPTITSREIQTAVRLVL 120

Query: 121 PGELAKHAVSEGTKAVTKFTSS 142
           PGELAKHAVSEGTKAVTKFTS+
Sbjct: 121 PGELAKHAVSEGTKAVTKFTSN 142

[Close]

BLAST of Carg28230-RA vs. ExPASy Swiss-Prot
Match: Q9LZT0 (Histone H2B.7 OS=Arabidopsis thaliana OX=3702 GN=At3g46030 PE=1 SV=3)

HSP 1 Score: 225.3 bits (573), Expect = 4.2e-58
Identity = 132/145 (91.03%), Postives = 135/145 (93.10%), Query Frame = 0


Query: 1   MAPKAEKKPAEKKPAEEK-KAEKAPAEKKPRAEKKLPKD---GADKKRKKAKKSVETYKI 60
           MAPKAEKKPAEKKP EEK KAEKAPAEKKP+A KKLPK+   G DKK+K  KKSVETYKI
Sbjct: 1   MAPKAEKKPAEKKPVEEKSKAEKAPAEKKPKAGKKLPKEAGAGGDKKKKMKKKSVETYKI 60

Query: 61  YIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREIQTAVR 120
           YIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLA ESSKLARYNKKPTITSREIQTAVR
Sbjct: 61  YIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLASESSKLARYNKKPTITSREIQTAVR 120

Query: 121 LVLPGELAKHAVSEGTKAVTKFTSS 142
           LVLPGELAKHAVSEGTKAVTKFTSS
Sbjct: 121 LVLPGELAKHAVSEGTKAVTKFTSS 145

[Close]

BLAST of Carg28230-RA vs. ExPASy Swiss-Prot
Match: P40283 (Histone H2B.11 OS=Arabidopsis thaliana OX=3702 GN=At5g59910 PE=1 SV=5)

HSP 1 Score: 223.8 bits (569), Expect = 1.2e-57
Identity = 132/150 (88.00%), Postives = 136/150 (90.67%), Query Frame = 0


Query: 1   MAPKAEKKPAEKKPAEEK------KAEKAPAEKKPRAEKKLPKD---GADKKRKKAKKSV 60
           MAPKAEKKPAEKKPA EK      KAEKAPAEKKP+A KKLPK+   G DKK+K  KKSV
Sbjct: 1   MAPKAEKKPAEKKPASEKPVEEKSKAEKAPAEKKPKAGKKLPKEAGAGGDKKKKMKKKSV 60

Query: 61  ETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREI 120
           ETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQE+SKLARYNKKPTITSREI
Sbjct: 61  ETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQEASKLARYNKKPTITSREI 120

Query: 121 QTAVRLVLPGELAKHAVSEGTKAVTKFTSS 142
           QTAVRLVLPGELAKHAVSEGTKAVTKFTSS
Sbjct: 121 QTAVRLVLPGELAKHAVSEGTKAVTKFTSS 150

[Close]

BLAST of Carg28230-RA vs. ExPASy Swiss-Prot
Match: O23629 (Histone H2B.6 OS=Arabidopsis thaliana OX=3702 GN=H2B PE=1 SV=3)

HSP 1 Score: 221.5 bits (563), Expect = 6.1e-57
Identity = 131/150 (87.33%), Postives = 135/150 (90.00%), Query Frame = 0


Query: 1   MAPKAEKKPAEKKPAEEK------KAEKAPAEKKPRAEKKLPKD---GADKKRKKAKKSV 60
           MAP+AEKKPAEKKPA EK      KAEKAPAEKKP+A KKLPK+   G DKK+K  KKSV
Sbjct: 1   MAPRAEKKPAEKKPAAEKPVEEKSKAEKAPAEKKPKAGKKLPKEAGAGGDKKKKMKKKSV 60

Query: 61  ETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREI 120
           ETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLA ESSKLARYNKKPTITSREI
Sbjct: 61  ETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLASESSKLARYNKKPTITSREI 120

Query: 121 QTAVRLVLPGELAKHAVSEGTKAVTKFTSS 142
           QTAVRLVLPGELAKHAVSEGTKAVTKFTSS
Sbjct: 121 QTAVRLVLPGELAKHAVSEGTKAVTKFTSS 150

[Close]

BLAST of Carg28230-RA vs. ExPASy TrEMBL
Match: A0A6J1FBJ0 (Histone H2B OS=Cucurbita moschata OX=3662 GN=LOC111443930 PE=3 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 5.9e-63
Identity = 140/141 (99.29%), Postives = 141/141 (100.00%), Query Frame = 0


Query: 1   MAPKAEKKPAEKKPAEEKKAEKAPAEKKPRAEKKLPKDGADKKRKKAKKSVETYKIYIFK 60
           MAPKAEKKPAEKKPAEEKKAEKAPAEKKPRAEKKLPKDGADKK+KKAKKSVETYKIYIFK
Sbjct: 1   MAPKAEKKPAEKKPAEEKKAEKAPAEKKPRAEKKLPKDGADKKKKKAKKSVETYKIYIFK 60

Query: 61  VLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREIQTAVRLVLP 120
           VLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREIQTAVRLVLP
Sbjct: 61  VLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREIQTAVRLVLP 120

Query: 121 GELAKHAVSEGTKAVTKFTSS 142
           GELAKHAVSEGTKAVTKFTSS
Sbjct: 121 GELAKHAVSEGTKAVTKFTSS 141

[Close]

BLAST of Carg28230-RA vs. ExPASy TrEMBL
Match: A0A6J1I2W4 (Histone H2B OS=Cucurbita maxima OX=3661 GN=LOC111468523 PE=3 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 5.9e-63
Identity = 140/141 (99.29%), Postives = 141/141 (100.00%), Query Frame = 0


Query: 1   MAPKAEKKPAEKKPAEEKKAEKAPAEKKPRAEKKLPKDGADKKRKKAKKSVETYKIYIFK 60
           MAPKAEKKPAEKKPAEEKKAEKAPAEKKPRAEKKLPKDGADKK+KKAKKSVETYKIYIFK
Sbjct: 1   MAPKAEKKPAEKKPAEEKKAEKAPAEKKPRAEKKLPKDGADKKKKKAKKSVETYKIYIFK 60

Query: 61  VLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREIQTAVRLVLP 120
           VLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREIQTAVRLVLP
Sbjct: 61  VLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREIQTAVRLVLP 120

Query: 121 GELAKHAVSEGTKAVTKFTSS 142
           GELAKHAVSEGTKAVTKFTSS
Sbjct: 121 GELAKHAVSEGTKAVTKFTSS 141

[Close]

BLAST of Carg28230-RA vs. ExPASy TrEMBL
Match: A0A6J1I068 (Histone H2B OS=Cucurbita maxima OX=3661 GN=LOC111468524 PE=3 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 4.3e-61
Identity = 139/141 (98.58%), Postives = 140/141 (99.29%), Query Frame = 0


Query: 1   MAPKAEKKPAEKKPAEEKKAEKAPAEKKPRAEKKLPKDGADKKRKKAKKSVETYKIYIFK 60
           MAPKAEKKPAEKKPAEEKKA+KAPAEKKPRAEKKLPKDGADKK KKAKKSVETYKIYIFK
Sbjct: 1   MAPKAEKKPAEKKPAEEKKADKAPAEKKPRAEKKLPKDGADKK-KKAKKSVETYKIYIFK 60

Query: 61  VLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREIQTAVRLVLP 120
           VLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREIQTAVRLVLP
Sbjct: 61  VLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREIQTAVRLVLP 120

Query: 121 GELAKHAVSEGTKAVTKFTSS 142
           GELAKHAVSEGTKAVTKFTSS
Sbjct: 121 GELAKHAVSEGTKAVTKFTSS 140

[Close]

BLAST of Carg28230-RA vs. ExPASy TrEMBL
Match: A0A6J1I594 (Histone H2B OS=Cucurbita maxima OX=3661 GN=LOC111469341 PE=3 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 2.1e-60
Identity = 137/142 (96.48%), Postives = 140/142 (98.59%), Query Frame = 0


Query: 1   MAPKAEKKPAEKKP-AEEKKAEKAPAEKKPRAEKKLPKDGADKKRKKAKKSVETYKIYIF 60
           MAPKAEKKPAEKKP AEEKKAEKAPAEKKPRAEKKLPKD +DKK+KKAKKS+ETYKIYIF
Sbjct: 1   MAPKAEKKPAEKKPAAEEKKAEKAPAEKKPRAEKKLPKDASDKKKKKAKKSIETYKIYIF 60

Query: 61  KVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREIQTAVRLVL 120
           KVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREIQTAVRLVL
Sbjct: 61  KVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREIQTAVRLVL 120

Query: 121 PGELAKHAVSEGTKAVTKFTSS 142
           PGELAKHAVSEGTKAVTKFTSS
Sbjct: 121 PGELAKHAVSEGTKAVTKFTSS 142

[Close]

BLAST of Carg28230-RA vs. ExPASy TrEMBL
Match: A0A6J1I3M0 (Histone H2B OS=Cucurbita maxima OX=3661 GN=LOC111469340 PE=3 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 2.1e-60
Identity = 137/142 (96.48%), Postives = 140/142 (98.59%), Query Frame = 0


Query: 1   MAPKAEKKPAEKKP-AEEKKAEKAPAEKKPRAEKKLPKDGADKKRKKAKKSVETYKIYIF 60
           MAPKAEKKPAEKKP AEEKKAEKAPAEKKPRAEKKLPKD ADKK+K+AKKS+ETYKIYIF
Sbjct: 1   MAPKAEKKPAEKKPAAEEKKAEKAPAEKKPRAEKKLPKDAADKKKKRAKKSIETYKIYIF 60

Query: 61  KVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREIQTAVRLVL 120
           KVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREIQTAVRLVL
Sbjct: 61  KVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREIQTAVRLVL 120

Query: 121 PGELAKHAVSEGTKAVTKFTSS 142
           PGELAKHAVSEGTKAVTKFTSS
Sbjct: 121 PGELAKHAVSEGTKAVTKFTSS 142

[Close]

BLAST of Carg28230-RA vs. TAIR 10
Match: AT3G46030.1 (Histone superfamily protein )

HSP 1 Score: 225.3 bits (573), Expect = 3.0e-59
Identity = 132/145 (91.03%), Postives = 135/145 (93.10%), Query Frame = 0


Query: 1   MAPKAEKKPAEKKPAEEK-KAEKAPAEKKPRAEKKLPKD---GADKKRKKAKKSVETYKI 60
           MAPKAEKKPAEKKP EEK KAEKAPAEKKP+A KKLPK+   G DKK+K  KKSVETYKI
Sbjct: 1   MAPKAEKKPAEKKPVEEKSKAEKAPAEKKPKAGKKLPKEAGAGGDKKKKMKKKSVETYKI 60

Query: 61  YIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREIQTAVR 120
           YIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLA ESSKLARYNKKPTITSREIQTAVR
Sbjct: 61  YIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLASESSKLARYNKKPTITSREIQTAVR 120

Query: 121 LVLPGELAKHAVSEGTKAVTKFTSS 142
           LVLPGELAKHAVSEGTKAVTKFTSS
Sbjct: 121 LVLPGELAKHAVSEGTKAVTKFTSS 145

[Close]

BLAST of Carg28230-RA vs. TAIR 10
Match: AT5G59910.1 (Histone superfamily protein )

HSP 1 Score: 223.8 bits (569), Expect = 8.8e-59
Identity = 132/150 (88.00%), Postives = 136/150 (90.67%), Query Frame = 0


Query: 1   MAPKAEKKPAEKKPAEEK------KAEKAPAEKKPRAEKKLPKD---GADKKRKKAKKSV 60
           MAPKAEKKPAEKKPA EK      KAEKAPAEKKP+A KKLPK+   G DKK+K  KKSV
Sbjct: 1   MAPKAEKKPAEKKPASEKPVEEKSKAEKAPAEKKPKAGKKLPKEAGAGGDKKKKMKKKSV 60

Query: 61  ETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREI 120
           ETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQE+SKLARYNKKPTITSREI
Sbjct: 61  ETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQEASKLARYNKKPTITSREI 120

Query: 121 QTAVRLVLPGELAKHAVSEGTKAVTKFTSS 142
           QTAVRLVLPGELAKHAVSEGTKAVTKFTSS
Sbjct: 121 QTAVRLVLPGELAKHAVSEGTKAVTKFTSS 150

[Close]

BLAST of Carg28230-RA vs. TAIR 10
Match: AT3G45980.1 (Histone superfamily protein )

HSP 1 Score: 221.5 bits (563), Expect = 4.4e-58
Identity = 131/150 (87.33%), Postives = 135/150 (90.00%), Query Frame = 0


Query: 1   MAPKAEKKPAEKKPAEEK------KAEKAPAEKKPRAEKKLPKD---GADKKRKKAKKSV 60
           MAP+AEKKPAEKKPA EK      KAEKAPAEKKP+A KKLPK+   G DKK+K  KKSV
Sbjct: 1   MAPRAEKKPAEKKPAAEKPVEEKSKAEKAPAEKKPKAGKKLPKEAGAGGDKKKKMKKKSV 60

Query: 61  ETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREI 120
           ETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLA ESSKLARYNKKPTITSREI
Sbjct: 61  ETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLASESSKLARYNKKPTITSREI 120

Query: 121 QTAVRLVLPGELAKHAVSEGTKAVTKFTSS 142
           QTAVRLVLPGELAKHAVSEGTKAVTKFTSS
Sbjct: 121 QTAVRLVLPGELAKHAVSEGTKAVTKFTSS 150

[Close]

BLAST of Carg28230-RA vs. TAIR 10
Match: AT2G28720.1 (Histone superfamily protein )

HSP 1 Score: 218.4 bits (555), Expect = 3.7e-57
Identity = 129/151 (85.43%), Postives = 135/151 (89.40%), Query Frame = 0


Query: 1   MAPKAEKKPAEKKPA------EEKKAEKAPAEKKPRAEKKLPKD----GADKKRKKAKKS 60
           MAPKA KKPAEKKPA      EEK AEKAPAEKKP+A KKLPK+    G +KK+K+ KKS
Sbjct: 1   MAPKAGKKPAEKKPAEKAPAEEEKVAEKAPAEKKPKAGKKLPKEAVTGGVEKKKKRVKKS 60

Query: 61  VETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSRE 120
            ETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQE+SKLARYNKKPTITSRE
Sbjct: 61  TETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQEASKLARYNKKPTITSRE 120

Query: 121 IQTAVRLVLPGELAKHAVSEGTKAVTKFTSS 142
           IQTAVRLVLPGELAKHAVSEGTKAVTKFTSS
Sbjct: 121 IQTAVRLVLPGELAKHAVSEGTKAVTKFTSS 151

[Close]

BLAST of Carg28230-RA vs. TAIR 10
Match: AT1G07790.1 (Histone superfamily protein )

HSP 1 Score: 217.6 bits (553), Expect = 6.3e-57
Identity = 128/148 (86.49%), Postives = 135/148 (91.22%), Query Frame = 0


Query: 1   MAPKAEKKPAEKKPA------EEKKAEKAPAEKKPRAEKKL-PKDGADKKRKKAKKSVET 60
           MAP+AEKKPAEKK A      E K AEKAPAEKKP+A KKL PK+  DKK+K++KK+VET
Sbjct: 1   MAPRAEKKPAEKKTAAERPVEENKAAEKAPAEKKPKAGKKLPPKEAGDKKKKRSKKNVET 60

Query: 61  YKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREIQT 120
           YKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREIQT
Sbjct: 61  YKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSKLARYNKKPTITSREIQT 120

Query: 121 AVRLVLPGELAKHAVSEGTKAVTKFTSS 142
           AVRLVLPGELAKHAVSEGTKAVTKFTSS
Sbjct: 121 AVRLVLPGELAKHAVSEGTKAVTKFTSS 148

The following BLAST results are available for this feature:

BLAST of Carg28230-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6586017.1	5.5e-63	100.00	hypothetical protein SDJN03_18750, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022937557.1	1.2e-62	99.29	histone H2B-like [Cucurbita moschata] >XP_022937558.1 histone H2B-like [Cucurbit...	[more]
XP_022969535.1	8.8e-61	98.58	histone H2B-like [Cucurbita maxima]	[more]
XP_022964661.1	4.4e-60	96.48	histone H2B.7-like [Cucurbita moschata] >XP_022970348.1 histone H2B.7-like [Cucu...	[more]
XP_030959009.1	4.4e-60	95.04	probable histone H2B.1 [Quercus lobata]	[more]

BLAST of Carg28230-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22582	1.1e-58	89.80	Histone H2B OS=Gossypium hirsutum OX=3635 GN=HIS2B PE=2 SV=3	[more]
O65821	1.9e-58	90.85	Histone H2B.1 OS=Solanum lycopersicum OX=4081 GN=H2B-1 PE=2 SV=4	[more]
Q9LZT0	4.2e-58	91.03	Histone H2B.7 OS=Arabidopsis thaliana OX=3702 GN=At3g46030 PE=1 SV=3	[more]
P40283	1.2e-57	88.00	Histone H2B.11 OS=Arabidopsis thaliana OX=3702 GN=At5g59910 PE=1 SV=5	[more]
O23629	6.1e-57	87.33	Histone H2B.6 OS=Arabidopsis thaliana OX=3702 GN=H2B PE=1 SV=3	[more]

BLAST of Carg28230-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FBJ0	5.9e-63	99.29	Histone H2B OS=Cucurbita moschata OX=3662 GN=LOC111443930 PE=3 SV=1	[more]
A0A6J1I2W4	5.9e-63	99.29	Histone H2B OS=Cucurbita maxima OX=3661 GN=LOC111468523 PE=3 SV=1	[more]
A0A6J1I068	4.3e-61	98.58	Histone H2B OS=Cucurbita maxima OX=3661 GN=LOC111468524 PE=3 SV=1	[more]
A0A6J1I594	2.1e-60	96.48	Histone H2B OS=Cucurbita maxima OX=3661 GN=LOC111469341 PE=3 SV=1	[more]
A0A6J1I3M0	2.1e-60	96.48	Histone H2B OS=Cucurbita maxima OX=3661 GN=LOC111469340 PE=3 SV=1	[more]

BLAST of Carg28230-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G46030.1	3.0e-59	91.03	Histone superfamily protein	[more]
AT5G59910.1	8.8e-59	88.00	Histone superfamily protein	[more]
AT3G45980.1	4.4e-58	87.33	Histone superfamily protein	[more]
AT2G28720.1	3.7e-57	85.43	Histone superfamily protein	[more]
AT1G07790.1	6.3e-57	86.49	Histone superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000558	Histone H2B	PRINTS	PR00621	HISTONEH2B	coord: 73..93 score: 75.74 coord: 112..125 score: 92.45 coord: 54..72 score: 80.55 coord: 125..138 score: 92.65 coord: 95..112 score: 76.51
IPR000558	Histone H2B	SMART	SM00427	h2b3	coord: 44..140 e-value: 1.8E-72 score: 256.7
IPR000558	Histone H2B	PANTHER	PTHR23428	HISTONE H2B	coord: 5..141
IPR000558	Histone H2B	PROSITE	PS00357	HISTONE_H2B	coord: 109..131
IPR007125	Histone H2A/H2B/H3	PFAM	PF00125	Histone	coord: 5..117 e-value: 1.8E-22 score: 80.1
IPR009072	Histone-fold	GENE3D	1.10.20.10	Histone, subunit A	coord: 2..141 e-value: 1.3E-58 score: 198.5
IPR009072	Histone-fold	SUPERFAMILY	47113	Histone-fold	coord: 26..141
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..49
None	No IPR available	PANTHER	PTHR23428:SF278	HISTONE H2B	coord: 5..141

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg28230	Carg28230	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg28230-RA:1	Carg28230-RA:1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg28230-RA:cds	Carg28230-RA:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg28230-RA	Carg28230-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006334	nucleosome assembly
cellular_component	GO:0000786	nucleosome
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0046982	protein heterodimerization activity