Carg23921-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg23921-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Villin-4
Location	Carg_Chr05: 4050789 .. 4053192 (-)
Sequence length	816
RNA-Seq Expression	Carg23921-RA
Synteny	Carg23921-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACACAGGTTTGGCGAGTCAGTGGCAACGAAAAGATTCTCATTCCTACCTCTGATCAGTCAAAATTTTACAGTGGGGATTGCTATATCTTTCAATACTCGTATCCTGGAGACGATAAAGATGAATATCTTATAGGAACGTGGTTTGGGAAGCAAAGCGTTGAGGTAATAAATGCTCAAAAAACTTCTAGATGCCTCAACCTGAAGAAAAAAAAAAAAAATCACACCGAAATTTAAAGTTACTTATCGTTCATTTATGCATTCAATACCTCACTTTAACATGATGGTTGATGGTTATGGGAGAAGTTAAAGTCGTAAATTATTGATATGTTAAAATATCTTTTGGAAACTATGAATTAGGAAGGAATGCTTCAACTGTAATTTGTTGTGTTGGGATAAATCCGTAACATCAATTTAAGTACACCTTTATGCTTCTTTCTGTTAGGTTTATAGTTTACCTTGACCAAGAATTTGGGACATGGTTTATCATTTGCTAGTTTGATTATAGGACTTCGAACATGATCTATTACTCATTAACAGATCTTGCATCTTAGATTTCAATCGTCCTTGTATGCTCTCTCTTTATGAGAGAAATTTTGGCTAAGAGTATTGAAATTAGAATAGGAGGCTCAAAAGTAGGACTTCTTCACATAATGGACACTCTTTTTTTTGTGTATCTAATGTTTCCCCCCTCATACTCTACACGCATTTTGTACTACATGAATACATTCTAATCTGAGGCCTTATTATTGTTGGGATTCGTTTTTGCTCATCTCCTCCAGGAAGAAAGAGCTACAGCATTATCACTGGTAAACAAGATGGTTGAGTCATTAAAGTTTCTCCCAGTCCAGGTAGGTTCATCACTTGGGGTTGTGATTTCCAATTGATGTACATTCATTTCTTTGGTATCTTCTTGTTTCTGATTCATTTCATTGTATTATAGGCTTGCCTTTACGAAGGGAATGAACCGATTCAATTTTATTCAATTTTCCAGAGCTTTGTTGTCTTCAAGGTTGGTTAGATATAATATAATCTGCAGTTAGAGAAAGTTGTAGGAGAATATTTTTTTCTTCTTATTCCATACATGAATTTTGATTTCCTAAATGATAATGTGATGAAATACATACTTCAGGGAGGCCTAAGTAAGGGATACAAGAATTATGTTGCGGAGAAGGAAATTCAAGATGAAACTTACCAAGAGGATGGGATTGCATTATTTAGAGTCCAGGGCTCTGGACCCGAAAATATGCAAGCAATACAAGTAGACCCGGTACTTGTACTATAGCATTTCGTTAGTTCTTTAATCATGCACACATATGACAAAGATGTCTTTAGTTAACATGATTGCAAAAGGTACAGTAGAAAGATTAAAGTTTATTTAATCTTTTCTATAGTCTTATCACTCTCCTCCATATGAGAAAAGGTATGTGTGAACCATTGGTATCAATTAGAGAGAAAATTTCACAAGAGCTCCTGAAATTGATTATCATAATAAGTCACTACTAAACATAGCATAACTAGCGTAACTCAAAGACTACATTGACATCATTTTGATCACTACTAAACTTGAAAGGTTAAATTAATAGATTATGATGTATTTAAATCTTTATGTATATATTCTTACCTTTCCCCATCTCTTTTCTCCCTCGGCAGGTTGCATCGTCTTTAAATTCCTCCTACTGCTACATATTGAATAGCAGCTCCTCTGTCTTTACATGGTCTGGAAGCCTCACAAACTCAGATAATCAAGAGCTTGTTGAGCGATTGCTGGATTTGATAAAGGTTGTGTAGTTCTCTCAGCTTGGACACAAGAGTTTGATTTCTAAACAGAGCTTCTTGACAAATGTGTTCTGTATGAAAAAAACCTGATTGCATAAATTTGTTGCAATCTATCATTTCACATCCATACGCCTTTCACTTTGGCAAAGAAGTCATGCTTATCTGAGTTTGAGTTTTGTTTAACAAGATAGGCCAGAGTTTTGTTTATCTCGTTACCTTTCTTTTTATTGATTTCTCACTTGTGTTTCAATTGCCAGCAGCCCAATGTACAATCTAGACCACAAAAAGAAGGTACAGAATCTGAACAATTTTGGAATTTGCTGGGAGGCAAATCTGAATATCCTAGCCAAAAAATCAGTCGAGAGGCTGAAAGTGATCCACACCTATTTTCTTGTACCTTCTCTGGAGGTTAGTATAATTCTTCCTGCTAGTGAAAAATTTATGCTTTGTCCTGTACTCTTCCATTCCGCTGTACTTCTAAATCTGACCCCTTTTCCTGTGAACTTGTGATGGCCATGATGAATTTGCAGGAAATTTGAAGGTACATTCTTTTCTCGTTCTGATCCATAAATTTCCTATAATTTATACTCTCATGTTCTTTCATTTCTTCTCAATACAAGCTTAG

mRNA sequence

ATGACACAGGTTTGGCGAGTCAGTGGCAACGAAAAGATTCTCATTCCTACCTCTGATCAGTCAAAATTTTACAGTGGGGATTGCTATATCTTTCAATACTCGTATCCTGGAGACGATAAAGATGAATATCTTATAGGAACGTGGTTTGGGAAGCAAAGCGTTGAGGAAGAAAGAGCTACAGCATTATCACTGGTAAACAAGATGGTTGAGTCATTAAAGTTTCTCCCAGTCCAGGCTTGCCTTTACGAAGGGAATGAACCGATTCAATTTTATTCAATTTTCCAGAGCTTTGTTGTCTTCAAGGGAGGCCTAAGTAAGGGATACAAGAATTATGTTGCGGAGAAGGAAATTCAAGATGAAACTTACCAAGAGGATGGGATTGCATTATTTAGAGTCCAGGGCTCTGGACCCGAAAATATGCAAGCAATACAAGTAGACCCGGTTGCATCGTCTTTAAATTCCTCCTACTGCTACATATTGAATAGCAGCTCCTCTGTCTTTACATGGTCTGGAAGCCTCACAAACTCAGATAATCAAGAGCTTGTTGAGCGATTGCTGGATTTGATAAAGCAGCCCAATGTACAATCTAGACCACAAAAAGAAGGTACAGAATCTGAACAATTTTGGAATTTGCTGGGAGGCAAATCTGAATATCCTAGCCAAAAAATCAGTCGAGAGGCTGAAAGTGATCCACACCTATTTTCTTGTACCTTCTCTGGAGGAAATTTGAAGGTACATTCTTTTCTCGTTCTGATCCATAAATTTCCTATAATTTATACTCTCATGTTCTTTCATTTCTTCTCAATACAAGCTTAG

Coding sequence (CDS)

ATGACACAGGTTTGGCGAGTCAGTGGCAACGAAAAGATTCTCATTCCTACCTCTGATCAGTCAAAATTTTACAGTGGGGATTGCTATATCTTTCAATACTCGTATCCTGGAGACGATAAAGATGAATATCTTATAGGAACGTGGTTTGGGAAGCAAAGCGTTGAGGAAGAAAGAGCTACAGCATTATCACTGGTAAACAAGATGGTTGAGTCATTAAAGTTTCTCCCAGTCCAGGCTTGCCTTTACGAAGGGAATGAACCGATTCAATTTTATTCAATTTTCCAGAGCTTTGTTGTCTTCAAGGGAGGCCTAAGTAAGGGATACAAGAATTATGTTGCGGAGAAGGAAATTCAAGATGAAACTTACCAAGAGGATGGGATTGCATTATTTAGAGTCCAGGGCTCTGGACCCGAAAATATGCAAGCAATACAAGTAGACCCGGTTGCATCGTCTTTAAATTCCTCCTACTGCTACATATTGAATAGCAGCTCCTCTGTCTTTACATGGTCTGGAAGCCTCACAAACTCAGATAATCAAGAGCTTGTTGAGCGATTGCTGGATTTGATAAAGCAGCCCAATGTACAATCTAGACCACAAAAAGAAGGTACAGAATCTGAACAATTTTGGAATTTGCTGGGAGGCAAATCTGAATATCCTAGCCAAAAAATCAGTCGAGAGGCTGAAAGTGATCCACACCTATTTTCTTGTACCTTCTCTGGAGGAAATTTGAAGGTACATTCTTTTCTCGTTCTGATCCATAAATTTCCTATAATTTATACTCTCATGTTCTTTCATTTCTTCTCAATACAAGCTTAG

Protein sequence

MTQVWRVSGNEKILIPTSDQSKFYSGDCYIFQYSYPGDDKDEYLIGTWFGKQSVEEERATALSLVNKMVESLKFLPVQACLYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDETYQEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQELVERLLDLIKQPNVQSRPQKEGTESEQFWNLLGGKSEYPSQKISREAESDPHLFSCTFSGGNLKVHSFLVLIHKFPIIYTLMFFHFFSIQA

Homology

[Close]

BLAST of Carg23921-RA vs. NCBI nr
Match: KAG7029831.1 (Villin-4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 545.0 bits (1403), Expect = 3.6e-151
Identity = 271/271 (100.00%), Postives = 271/271 (100.00%), Query Frame = 0


Query: 1   MTQVWRVSGNEKILIPTSDQSKFYSGDCYIFQYSYPGDDKDEYLIGTWFGKQSVEEERAT 60
           MTQVWRVSGNEKILIPTSDQSKFYSGDCYIFQYSYPGDDKDEYLIGTWFGKQSVEEERAT
Sbjct: 1   MTQVWRVSGNEKILIPTSDQSKFYSGDCYIFQYSYPGDDKDEYLIGTWFGKQSVEEERAT 60

Query: 61  ALSLVNKMVESLKFLPVQACLYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDE 120
           ALSLVNKMVESLKFLPVQACLYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDE
Sbjct: 61  ALSLVNKMVESLKFLPVQACLYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDE 120

Query: 121 TYQEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQE 180
           TYQEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQE
Sbjct: 121 TYQEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQE 180

Query: 181 LVERLLDLIKQPNVQSRPQKEGTESEQFWNLLGGKSEYPSQKISREAESDPHLFSCTFSG 240
           LVERLLDLIKQPNVQSRPQKEGTESEQFWNLLGGKSEYPSQKISREAESDPHLFSCTFSG
Sbjct: 181 LVERLLDLIKQPNVQSRPQKEGTESEQFWNLLGGKSEYPSQKISREAESDPHLFSCTFSG 240

Query: 241 GNLKVHSFLVLIHKFPIIYTLMFFHFFSIQA 272
           GNLKVHSFLVLIHKFPIIYTLMFFHFFSIQA
Sbjct: 241 GNLKVHSFLVLIHKFPIIYTLMFFHFFSIQA 271

[Close]

BLAST of Carg23921-RA vs. NCBI nr
Match: XP_022997656.1 (villin-4-like isoform X1 [Cucurbita maxima] >XP_022997657.1 villin-4-like isoform X1 [Cucurbita maxima] >XP_022997658.1 villin-4-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 486.1 bits (1250), Expect = 2.0e-133
Identity = 240/243 (98.77%), Postives = 242/243 (99.59%), Query Frame = 0


Query: 3   QVWRVSGNEKILIPTSDQSKFYSGDCYIFQYSYPGDDKDEYLIGTWFGKQSVEEERATAL 62
           QVWRVSGNEKILIPTSDQSKFYSGDCYIFQYSYPGDDKDEYLIGTWFGK+SVEEERATAL
Sbjct: 395 QVWRVSGNEKILIPTSDQSKFYSGDCYIFQYSYPGDDKDEYLIGTWFGKRSVEEERATAL 454

Query: 63  SLVNKMVESLKFLPVQACLYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDETY 122
           SLVNKMVESLKFLPVQA LYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDETY
Sbjct: 455 SLVNKMVESLKFLPVQARLYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDETY 514

Query: 123 QEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQELV 182
           QEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQELV
Sbjct: 515 QEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQELV 574

Query: 183 ERLLDLIKQPNVQSRPQKEGTESEQFWNLLGGKSEYPSQKISREAESDPHLFSCTFSGGN 242
           ERLLDLIKQPNVQSRPQKEGTESEQFWNLLGGKSEYPSQK+SREAESDPHLFSCTFSGGN
Sbjct: 575 ERLLDLIKQPNVQSRPQKEGTESEQFWNLLGGKSEYPSQKVSREAESDPHLFSCTFSGGN 634

Query: 243 LKV 246
           LKV
Sbjct: 635 LKV 637

[Close]

BLAST of Carg23921-RA vs. NCBI nr
Match: KAG6598881.1 (Villin-4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 486.1 bits (1250), Expect = 2.0e-133
Identity = 242/243 (99.59%), Postives = 242/243 (99.59%), Query Frame = 0


Query: 3   QVWRVSGNEKILIPTSDQSKFYSGDCYIFQYSYPGDDKDEYLIGTWFGKQSVEEERATAL 62
           QVWRVSGNEKILIPTSDQSKFYSGDCYIFQYSYPGDDKDEYLIGTWFGKQSVEEERATAL
Sbjct: 400 QVWRVSGNEKILIPTSDQSKFYSGDCYIFQYSYPGDDKDEYLIGTWFGKQSVEEERATAL 459

Query: 63  SLVNKMVESLKFLPVQACLYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDETY 122
           SLVNKMVESLKFLPVQACLYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDETY
Sbjct: 460 SLVNKMVESLKFLPVQACLYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDETY 519

Query: 123 QEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQELV 182
           QEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQELV
Sbjct: 520 QEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQELV 579

Query: 183 ERLLDLIKQPNVQSRPQKEGTESEQFWNLLGGKSEYPSQKISREAESDPHLFSCTFSGGN 242
           ERLLDLIK PNVQSRPQKEGTESEQFWNLLGGKSEYPSQKISREAESDPHLFSCTFSGGN
Sbjct: 580 ERLLDLIK-PNVQSRPQKEGTESEQFWNLLGGKSEYPSQKISREAESDPHLFSCTFSGGN 639

Query: 243 LKV 246
           LKV
Sbjct: 640 LKV 641

[Close]

BLAST of Carg23921-RA vs. NCBI nr
Match: XP_022929553.1 (villin-4-like isoform X1 [Cucurbita moschata] >XP_022929554.1 villin-4-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 485.7 bits (1249), Expect = 2.6e-133
Identity = 241/243 (99.18%), Postives = 241/243 (99.18%), Query Frame = 0


Query: 3   QVWRVSGNEKILIPTSDQSKFYSGDCYIFQYSYPGDDKDEYLIGTWFGKQSVEEERATAL 62
           QVWRVSGNEKILIP SDQSKFYSGDCYIFQYSYPGDDKDEYLIGTWFGKQSVEEERATAL
Sbjct: 395 QVWRVSGNEKILIPISDQSKFYSGDCYIFQYSYPGDDKDEYLIGTWFGKQSVEEERATAL 454

Query: 63  SLVNKMVESLKFLPVQACLYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDETY 122
           SLVNKMVESLKFLPVQA LYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDETY
Sbjct: 455 SLVNKMVESLKFLPVQARLYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDETY 514

Query: 123 QEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQELV 182
           QEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQELV
Sbjct: 515 QEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQELV 574

Query: 183 ERLLDLIKQPNVQSRPQKEGTESEQFWNLLGGKSEYPSQKISREAESDPHLFSCTFSGGN 242
           ERLLDLIKQPNVQSRPQKEGTESEQFWNLLGGKSEYPSQKISREAESDPHLFSCTFSGGN
Sbjct: 575 ERLLDLIKQPNVQSRPQKEGTESEQFWNLLGGKSEYPSQKISREAESDPHLFSCTFSGGN 634

Query: 243 LKV 246
           LKV
Sbjct: 635 LKV 637

[Close]

BLAST of Carg23921-RA vs. NCBI nr
Match: XP_023521363.1 (villin-4-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023545663.1 villin-4-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 484.6 bits (1246), Expect = 5.7e-133
Identity = 240/243 (98.77%), Postives = 241/243 (99.18%), Query Frame = 0


Query: 3   QVWRVSGNEKILIPTSDQSKFYSGDCYIFQYSYPGDDKDEYLIGTWFGKQSVEEERATAL 62
           QVWRVSGNEKILIPTSDQSKFYSGDCYIFQYSYPGDDKDEYLIGTWFGKQSVEEERATAL
Sbjct: 395 QVWRVSGNEKILIPTSDQSKFYSGDCYIFQYSYPGDDKDEYLIGTWFGKQSVEEERATAL 454

Query: 63  SLVNKMVESLKFLPVQACLYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDETY 122
           SLVNKMVESLKFLPVQA LYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDETY
Sbjct: 455 SLVNKMVESLKFLPVQARLYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDETY 514

Query: 123 QEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQELV 182
           QEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQELV
Sbjct: 515 QEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQELV 574

Query: 183 ERLLDLIKQPNVQSRPQKEGTESEQFWNLLGGKSEYPSQKISREAESDPHLFSCTFSGGN 242
           ERLLDLIKQPNVQSR QKEGTESEQFWNLLGGKSEYPSQK+SREAESDPHLFSCTFSGGN
Sbjct: 575 ERLLDLIKQPNVQSRSQKEGTESEQFWNLLGGKSEYPSQKVSREAESDPHLFSCTFSGGN 634

Query: 243 LKV 246
           LKV
Sbjct: 635 LKV 637

[Close]

BLAST of Carg23921-RA vs. ExPASy Swiss-Prot
Match: O65570 (Villin-4 OS=Arabidopsis thaliana OX=3702 GN=VLN4 PE=1 SV=1)

HSP 1 Score: 364.8 bits (935), Expect = 8.6e-100
Identity = 172/243 (70.78%), Postives = 209/243 (86.01%), Query Frame = 0


Query: 3   QVWRVSGNEKILIPTSDQSKFYSGDCYIFQYSYPGDDKDEYLIGTWFGKQSVEEERATAL 62
           QVWRV+G  K L+  +D SKFYSGDCY+FQYSYPG++K+E LIGTWFGKQSVEEER +A+
Sbjct: 395 QVWRVNGQAKTLLQAADHSKFYSGDCYVFQYSYPGEEKEEVLIGTWFGKQSVEEERGSAV 454

Query: 63  SLVNKMVESLKFLPVQACLYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDETY 122
           S+ +KMVES+KF+P QA +YEG EPIQF+ I QSF+VFKGG+S GYK Y+AEKE+ D+TY
Sbjct: 455 SMASKMVESMKFVPAQARIYEGKEPIQFFVIMQSFIVFKGGISSGYKKYIAEKEVDDDTY 514

Query: 123 QEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQELV 182
            E+G+ALFR+QGSGPENMQAIQVDPVA+SLNSSY YIL++ SSVFTW+G+L+ + +QEL 
Sbjct: 515 NENGVALFRIQGSGPENMQAIQVDPVAASLNSSYYYILHNDSSVFTWAGNLSTATDQELA 574

Query: 183 ERLLDLIKQPNVQSRPQKEGTESEQFWNLLGGKSEYPSQKISREAESDPHLFSCTFSGGN 242
           ER LDLIK PN QSR QKEG+ESEQFW LLGGK+EY SQK+++E E DPHLFSCTF+   
Sbjct: 575 ERQLDLIK-PNQQSRAQKEGSESEQFWELLGGKAEYSSQKLTKEPERDPHLFSCTFTKEV 634

Query: 243 LKV 246
           LKV
Sbjct: 635 LKV 636

[Close]

BLAST of Carg23921-RA vs. ExPASy Swiss-Prot
Match: Q0J716 (Villin-5 OS=Oryza sativa subsp. japonica OX=39947 GN=VLN5 PE=3 SV=1)

HSP 1 Score: 359.0 bits (920), Expect = 4.7e-98
Identity = 172/243 (70.78%), Postives = 205/243 (84.36%), Query Frame = 0


Query: 3   QVWRVSGNEKILIPTSDQSKFYSGDCYIFQYSYPGDDKDEYLIGTWFGKQSVEEERATAL 62
           QVWR++  +KIL+P++DQSKFY+GDCYIFQY YPGDDK+E LIG+WFGK+S+EE+R TA+
Sbjct: 398 QVWRINDKDKILLPSADQSKFYTGDCYIFQYMYPGDDKEECLIGSWFGKKSIEEDRVTAI 457

Query: 63  SLVNKMVESLKFLPVQACLYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDETY 122
           SL +KMVES KF  VQ  LYEG EPIQF+ IFQSF VFKGGLS GYK ++AE  I D+TY
Sbjct: 458 SLASKMVESAKFQAVQTRLYEGKEPIQFFVIFQSFQVFKGGLSSGYKKFIAENGIDDDTY 517

Query: 123 QEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQELV 182
            EDG+ALFR+QGSGPENMQAIQVD  ASSLNSSY YIL+  ++VFTW+G+LT S +QE+V
Sbjct: 518 LEDGLALFRIQGSGPENMQAIQVDAAASSLNSSYSYILHDGNTVFTWTGNLTTSLDQEVV 577

Query: 183 ERLLDLIKQPNVQSRPQKEGTESEQFWNLLGGKSEYPSQKISREAESDPHLFSCTFSGGN 242
           ER LD+IK PN QSR QKEG+E++QFW+LLGGKSEYPSQKI R  ESDPHLFSC    GN
Sbjct: 578 ERQLDIIK-PNSQSRSQKEGSETDQFWSLLGGKSEYPSQKIGRANESDPHLFSCILPKGN 637

Query: 243 LKV 246
           LK+
Sbjct: 638 LKI 639

[Close]

BLAST of Carg23921-RA vs. ExPASy Swiss-Prot
Match: Q9LVC6 (Villin-5 OS=Arabidopsis thaliana OX=3702 GN=VLN5 PE=2 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 4.4e-96
Identity = 162/242 (66.94%), Postives = 207/242 (85.54%), Query Frame = 0


Query: 3   QVWRVSGNEKILIPTSDQSKFYSGDCYIFQYSYPGDDKDEYLIGTWFGKQSVEEERATAL 62
           QVWR++  EKIL+  ++QSKFYSGDCYI QYSYPG+D++E+L+GTWFGKQSVEE+RA+A+
Sbjct: 397 QVWRINCEEKILLEAAEQSKFYSGDCYILQYSYPGEDREEHLVGTWFGKQSVEEDRASAI 456

Query: 63  SLVNKMVESLKFLPVQACLYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDETY 122
           SL NKMVES+KF+P QA + EG EPIQF+ I QSF+ FKGG+S  +K Y+AE +I D TY
Sbjct: 457 SLANKMVESMKFVPAQARINEGKEPIQFFVIMQSFITFKGGVSDAFKKYIAENDIPDTTY 516

Query: 123 QEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQELV 182
           + +G+ALFRVQGSGPENMQAIQ++  ++ LNSS+CYIL+  S+VFTW G+LT+S++QEL+
Sbjct: 517 EAEGVALFRVQGSGPENMQAIQIEAASAGLNSSHCYILHGDSTVFTWCGNLTSSEDQELM 576

Query: 183 ERLLDLIKQPNVQSRPQKEGTESEQFWNLLGGKSEYPSQKISREAESDPHLFSCTFSGGN 242
           ER+LDLIK PN  ++ QKEG+ESEQFW LLGGKSEYPSQKI R+ ESDPHLFSCT++  +
Sbjct: 577 ERMLDLIK-PNEPTKAQKEGSESEQFWELLGGKSEYPSQKIKRDGESDPHLFSCTYTNES 636

Query: 243 LK 245
           LK
Sbjct: 637 LK 637

[Close]

BLAST of Carg23921-RA vs. ExPASy Swiss-Prot
Match: Q67U26 (Villin-3 OS=Oryza sativa subsp. japonica OX=39947 GN=VLN3 PE=2 SV=2)

HSP 1 Score: 340.1 bits (871), Expect = 2.3e-92
Identity = 162/243 (66.67%), Postives = 202/243 (83.13%), Query Frame = 0


Query: 3   QVWRVSGNEKILIPTSDQSKFYSGDCYIFQYSYPGDDKDEYLIGTWFGKQSVEEERATAL 62
           QVWRV+G EK  +  S+Q KFYSGDCYIFQYSYPG++ +E LIGTWFGK+SV++E+ TA+
Sbjct: 397 QVWRVNGTEKTFLSFSEQCKFYSGDCYIFQYSYPGEEGEECLIGTWFGKKSVQDEKTTAI 456

Query: 63  SLVNKMVESLKFLPVQACLYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDETY 122
           S+ +KMVESLKF  V   LYEG EP +F+SIFQ+ V+FKGG+S GYK +V+E  I+D+TY
Sbjct: 457 SVASKMVESLKFQAVMVRLYEGKEPAEFFSIFQNLVIFKGGVSTGYKKFVSENGIEDDTY 516

Query: 123 QEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQELV 182
            E+G+ALFRVQGSGPENMQAIQVD  A+SLNSSYCY+L+   ++FTW G+L++S +QEL 
Sbjct: 517 SENGVALFRVQGSGPENMQAIQVDTAATSLNSSYCYVLHDGDTLFTWIGNLSSSMDQELA 576

Query: 183 ERLLDLIKQPNVQSRPQKEGTESEQFWNLLGGKSEYPSQKISREAESDPHLFSCTFSGGN 242
           ER LD+IK PN+QSR  KEG+E +QFW LLG KSEYPSQKI+++ ESDPHLFSCTFS G 
Sbjct: 577 ERQLDVIK-PNLQSRMLKEGSEYDQFWKLLGVKSEYPSQKIAKDQESDPHLFSCTFSKGV 636

Query: 243 LKV 246
           LKV
Sbjct: 637 LKV 638

[Close]

BLAST of Carg23921-RA vs. ExPASy Swiss-Prot
Match: B8ATT7 (Villin-4 OS=Oryza sativa subsp. indica OX=39946 GN=VLN4 PE=3 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 1.5e-88
Identity = 162/253 (64.03%), Postives = 200/253 (79.05%), Query Frame = 0


Query: 3   QVWRVSGNEKILIPTSDQSKFYSGDCYIFQYSYPGDDKDEYLIGTWFGKQSVEEERATAL 62
           QVWRV+G+ K L+ +SDQSK Y+GDCYIFQY+Y GDDK+E LIGTWFGK+SVEE+R +A+
Sbjct: 398 QVWRVNGDGKTLLSSSDQSKLYTGDCYIFQYTYTGDDKEECLIGTWFGKKSVEEDRTSAI 457

Query: 63  SLVNKMVESLKFLPVQACLYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDETY 122
           SL +KM ++ KF   QA LYEG EPIQF+ IFQS  VFKGGLS GYKN++A     D+TY
Sbjct: 458 SLASKMFQAAKFQAAQARLYEGKEPIQFFVIFQSLQVFKGGLSSGYKNFIAVNGTDDDTY 517

Query: 123 QEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQELV 182
            E G+ALFR+QGSG ENMQAIQVD V+SSLNSSYCYIL++ ++VFTW+G+LT S + +LV
Sbjct: 518 VEGGLALFRIQGSGSENMQAIQVDAVSSSLNSSYCYILHNGNTVFTWTGNLTTSLDNDLV 577

Query: 183 ERLLDLIKQPNVQSRPQKEGTESEQFWNLLGGKSEYPSQKISREAESDPHLFSCTFSGGN 242
           ER LD+IK P++ SR QKEG E++QFW LLGGK +Y ++KI +E ESDPHLFSC  S  N
Sbjct: 578 ERQLDVIK-PDLPSRSQKEGRETDQFWELLGGKCKYSNKKIGKENESDPHLFSCILSKEN 637

Query: 243 LKVHSFLVLIHKF 256
           LKV      IH F
Sbjct: 638 LKVKE----IHHF 645

[Close]

BLAST of Carg23921-RA vs. ExPASy TrEMBL
Match: A0A6J1K5Q4 (villin-4-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492524 PE=4 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 9.5e-134
Identity = 240/243 (98.77%), Postives = 242/243 (99.59%), Query Frame = 0


Query: 3   QVWRVSGNEKILIPTSDQSKFYSGDCYIFQYSYPGDDKDEYLIGTWFGKQSVEEERATAL 62
           QVWRVSGNEKILIPTSDQSKFYSGDCYIFQYSYPGDDKDEYLIGTWFGK+SVEEERATAL
Sbjct: 395 QVWRVSGNEKILIPTSDQSKFYSGDCYIFQYSYPGDDKDEYLIGTWFGKRSVEEERATAL 454

Query: 63  SLVNKMVESLKFLPVQACLYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDETY 122
           SLVNKMVESLKFLPVQA LYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDETY
Sbjct: 455 SLVNKMVESLKFLPVQARLYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDETY 514

Query: 123 QEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQELV 182
           QEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQELV
Sbjct: 515 QEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQELV 574

Query: 183 ERLLDLIKQPNVQSRPQKEGTESEQFWNLLGGKSEYPSQKISREAESDPHLFSCTFSGGN 242
           ERLLDLIKQPNVQSRPQKEGTESEQFWNLLGGKSEYPSQK+SREAESDPHLFSCTFSGGN
Sbjct: 575 ERLLDLIKQPNVQSRPQKEGTESEQFWNLLGGKSEYPSQKVSREAESDPHLFSCTFSGGN 634

Query: 243 LKV 246
           LKV
Sbjct: 635 LKV 637

[Close]

BLAST of Carg23921-RA vs. ExPASy TrEMBL
Match: A0A6J1EP34 (villin-4-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436105 PE=4 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 1.2e-133
Identity = 241/243 (99.18%), Postives = 241/243 (99.18%), Query Frame = 0


Query: 3   QVWRVSGNEKILIPTSDQSKFYSGDCYIFQYSYPGDDKDEYLIGTWFGKQSVEEERATAL 62
           QVWRVSGNEKILIP SDQSKFYSGDCYIFQYSYPGDDKDEYLIGTWFGKQSVEEERATAL
Sbjct: 395 QVWRVSGNEKILIPISDQSKFYSGDCYIFQYSYPGDDKDEYLIGTWFGKQSVEEERATAL 454

Query: 63  SLVNKMVESLKFLPVQACLYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDETY 122
           SLVNKMVESLKFLPVQA LYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDETY
Sbjct: 455 SLVNKMVESLKFLPVQARLYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDETY 514

Query: 123 QEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQELV 182
           QEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQELV
Sbjct: 515 QEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQELV 574

Query: 183 ERLLDLIKQPNVQSRPQKEGTESEQFWNLLGGKSEYPSQKISREAESDPHLFSCTFSGGN 242
           ERLLDLIKQPNVQSRPQKEGTESEQFWNLLGGKSEYPSQKISREAESDPHLFSCTFSGGN
Sbjct: 575 ERLLDLIKQPNVQSRPQKEGTESEQFWNLLGGKSEYPSQKISREAESDPHLFSCTFSGGN 634

Query: 243 LKV 246
           LKV
Sbjct: 635 LKV 637

[Close]

BLAST of Carg23921-RA vs. ExPASy TrEMBL
Match: A0A6J1KAG3 (villin-4-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111492524 PE=4 SV=1)

HSP 1 Score: 479.6 bits (1233), Expect = 8.9e-132
Identity = 239/243 (98.35%), Postives = 241/243 (99.18%), Query Frame = 0


Query: 3   QVWRVSGNEKILIPTSDQSKFYSGDCYIFQYSYPGDDKDEYLIGTWFGKQSVEEERATAL 62
           QVWRVSGNEKILIPTSDQSKFYSGDCYIFQYSYPGDDKDEYLIGTWFGK+SVEEERATAL
Sbjct: 395 QVWRVSGNEKILIPTSDQSKFYSGDCYIFQYSYPGDDKDEYLIGTWFGKRSVEEERATAL 454

Query: 63  SLVNKMVESLKFLPVQACLYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDETY 122
           SLVNKMVESLKFLPVQA LYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDETY
Sbjct: 455 SLVNKMVESLKFLPVQARLYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDETY 514

Query: 123 QEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQELV 182
           QEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQELV
Sbjct: 515 QEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQELV 574

Query: 183 ERLLDLIKQPNVQSRPQKEGTESEQFWNLLGGKSEYPSQKISREAESDPHLFSCTFSGGN 242
           ERLLDLIK PNVQSRPQKEGTESEQFWNLLGGKSEYPSQK+SREAESDPHLFSCTFSGGN
Sbjct: 575 ERLLDLIK-PNVQSRPQKEGTESEQFWNLLGGKSEYPSQKVSREAESDPHLFSCTFSGGN 634

Query: 243 LKV 246
           LKV
Sbjct: 635 LKV 636

[Close]

BLAST of Carg23921-RA vs. ExPASy TrEMBL
Match: A0A6J1EUQ1 (villin-4-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111436105 PE=4 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 1.2e-131
Identity = 240/243 (98.77%), Postives = 240/243 (98.77%), Query Frame = 0


Query: 3   QVWRVSGNEKILIPTSDQSKFYSGDCYIFQYSYPGDDKDEYLIGTWFGKQSVEEERATAL 62
           QVWRVSGNEKILIP SDQSKFYSGDCYIFQYSYPGDDKDEYLIGTWFGKQSVEEERATAL
Sbjct: 395 QVWRVSGNEKILIPISDQSKFYSGDCYIFQYSYPGDDKDEYLIGTWFGKQSVEEERATAL 454

Query: 63  SLVNKMVESLKFLPVQACLYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDETY 122
           SLVNKMVESLKFLPVQA LYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDETY
Sbjct: 455 SLVNKMVESLKFLPVQARLYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDETY 514

Query: 123 QEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQELV 182
           QEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQELV
Sbjct: 515 QEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQELV 574

Query: 183 ERLLDLIKQPNVQSRPQKEGTESEQFWNLLGGKSEYPSQKISREAESDPHLFSCTFSGGN 242
           ERLLDLIK PNVQSRPQKEGTESEQFWNLLGGKSEYPSQKISREAESDPHLFSCTFSGGN
Sbjct: 575 ERLLDLIK-PNVQSRPQKEGTESEQFWNLLGGKSEYPSQKISREAESDPHLFSCTFSGGN 634

Query: 243 LKV 246
           LKV
Sbjct: 635 LKV 636

[Close]

BLAST of Carg23921-RA vs. ExPASy TrEMBL
Match: A0A6J1FCF4 (villin-4 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111444348 PE=3 SV=1)

HSP 1 Score: 468.0 bits (1203), Expect = 2.7e-128
Identity = 232/253 (91.70%), Postives = 243/253 (96.05%), Query Frame = 0


Query: 3   QVWRVSGNEKILIPTSDQSKFYSGDCYIFQYSYPGDDKDEYLIGTWFGKQSVEEERATAL 62
           QVWRVSGNEKILIP SDQSKFYSGDCYIFQYSYPGDD+DEYLIGTWFGKQSVEEERA+AL
Sbjct: 395 QVWRVSGNEKILIPASDQSKFYSGDCYIFQYSYPGDDRDEYLIGTWFGKQSVEEERASAL 454

Query: 63  SLVNKMVESLKFLPVQACLYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDETY 122
           SLVNKMVESLKFLPVQA +YEG+EPIQF+SIFQ FVVFKGGLSKGYKNYVAEKEIQDETY
Sbjct: 455 SLVNKMVESLKFLPVQARIYEGSEPIQFHSIFQGFVVFKGGLSKGYKNYVAEKEIQDETY 514

Query: 123 QEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQELV 182
           QEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSS+VFTW+GSLT+SDNQELV
Sbjct: 515 QEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSTVFTWTGSLTSSDNQELV 574

Query: 183 ERLLDLIKQPNVQSRPQKEGTESEQFWNLLGGKSEYPSQKISREAESDPHLFSCTFSGGN 242
           ERLLDLIKQPNVQSRPQKEGTESEQFWNLLGGKSEYPSQKI+R+AESDPHLFSCTFS GN
Sbjct: 575 ERLLDLIKQPNVQSRPQKEGTESEQFWNLLGGKSEYPSQKIARDAESDPHLFSCTFSRGN 634

Query: 243 LKVHSFLVLIHKF 256
           LKV    V +H F
Sbjct: 635 LKV----VEVHNF 643

[Close]

BLAST of Carg23921-RA vs. TAIR 10
Match: AT4G30160.1 (villin 4 )

HSP 1 Score: 364.8 bits (935), Expect = 6.1e-101
Identity = 172/243 (70.78%), Postives = 209/243 (86.01%), Query Frame = 0


Query: 3   QVWRVSGNEKILIPTSDQSKFYSGDCYIFQYSYPGDDKDEYLIGTWFGKQSVEEERATAL 62
           QVWRV+G  K L+  +D SKFYSGDCY+FQYSYPG++K+E LIGTWFGKQSVEEER +A+
Sbjct: 395 QVWRVNGQAKTLLQAADHSKFYSGDCYVFQYSYPGEEKEEVLIGTWFGKQSVEEERGSAV 454

Query: 63  SLVNKMVESLKFLPVQACLYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDETY 122
           S+ +KMVES+KF+P QA +YEG EPIQF+ I QSF+VFKGG+S GYK Y+AEKE+ D+TY
Sbjct: 455 SMASKMVESMKFVPAQARIYEGKEPIQFFVIMQSFIVFKGGISSGYKKYIAEKEVDDDTY 514

Query: 123 QEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQELV 182
            E+G+ALFR+QGSGPENMQAIQVDPVA+SLNSSY YIL++ SSVFTW+G+L+ + +QEL 
Sbjct: 515 NENGVALFRIQGSGPENMQAIQVDPVAASLNSSYYYILHNDSSVFTWAGNLSTATDQELA 574

Query: 183 ERLLDLIKQPNVQSRPQKEGTESEQFWNLLGGKSEYPSQKISREAESDPHLFSCTFSGGN 242
           ER LDLIK PN QSR QKEG+ESEQFW LLGGK+EY SQK+++E E DPHLFSCTF+   
Sbjct: 575 ERQLDLIK-PNQQSRAQKEGSESEQFWELLGGKAEYSSQKLTKEPERDPHLFSCTFTKEV 634

Query: 243 LKV 246
           LKV
Sbjct: 635 LKV 636

[Close]

BLAST of Carg23921-RA vs. TAIR 10
Match: AT4G30160.2 (villin 4 )

HSP 1 Score: 364.8 bits (935), Expect = 6.1e-101
Identity = 173/247 (70.04%), Postives = 210/247 (85.02%), Query Frame = 0


Query: 3   QVWRVSGNEKILIPTSDQSKFYSGDCYIFQYSYPGDDKDEYLIGTWFGKQSVEEERATAL 62
           QVWRV+G  K L+  +D SKFYSGDCY+FQYSYPG++K+E LIGTWFGKQSVEEER +A+
Sbjct: 395 QVWRVNGQAKTLLQAADHSKFYSGDCYVFQYSYPGEEKEEVLIGTWFGKQSVEEERGSAV 454

Query: 63  SLVNKMVESLKFLPVQACLYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDETY 122
           S+ +KMVES+KF+P QA +YEG EPIQF+ I QSF+VFKGG+S GYK Y+AEKE+ D+TY
Sbjct: 455 SMASKMVESMKFVPAQARIYEGKEPIQFFVIMQSFIVFKGGISSGYKKYIAEKEVDDDTY 514

Query: 123 QEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQELV 182
            E+G+ALFR+QGSGPENMQAIQVDPVA+SLNSSY YIL++ SSVFTW+G+L+ + +QEL 
Sbjct: 515 NENGVALFRIQGSGPENMQAIQVDPVAASLNSSYYYILHNDSSVFTWAGNLSTATDQELA 574

Query: 183 ERLLDLIKQPNVQSRPQKEGTESEQFWNLLGGKSEYPSQKISREAESDPHLFSCTFSGGN 242
           ER LDLIK PN QSR QKEG+ESEQFW LLGGK+EY SQK+++E E DPHLFSCTF+   
Sbjct: 575 ERQLDLIK-PNQQSRAQKEGSESEQFWELLGGKAEYSSQKLTKEPERDPHLFSCTFTKEV 634

Query: 243 LKVHSFL 250
           LKV   L
Sbjct: 635 LKVRILL 640

[Close]

BLAST of Carg23921-RA vs. TAIR 10
Match: AT5G57320.1 (villin, putative )

HSP 1 Score: 352.4 bits (903), Expect = 3.1e-97
Identity = 162/242 (66.94%), Postives = 207/242 (85.54%), Query Frame = 0


Query: 3   QVWRVSGNEKILIPTSDQSKFYSGDCYIFQYSYPGDDKDEYLIGTWFGKQSVEEERATAL 62
           QVWR++  EKIL+  ++QSKFYSGDCYI QYSYPG+D++E+L+GTWFGKQSVEE+RA+A+
Sbjct: 397 QVWRINCEEKILLEAAEQSKFYSGDCYILQYSYPGEDREEHLVGTWFGKQSVEEDRASAI 456

Query: 63  SLVNKMVESLKFLPVQACLYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDETY 122
           SL NKMVES+KF+P QA + EG EPIQF+ I QSF+ FKGG+S  +K Y+AE +I D TY
Sbjct: 457 SLANKMVESMKFVPAQARINEGKEPIQFFVIMQSFITFKGGVSDAFKKYIAENDIPDTTY 516

Query: 123 QEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQELV 182
           + +G+ALFRVQGSGPENMQAIQ++  ++ LNSS+CYIL+  S+VFTW G+LT+S++QEL+
Sbjct: 517 EAEGVALFRVQGSGPENMQAIQIEAASAGLNSSHCYILHGDSTVFTWCGNLTSSEDQELM 576

Query: 183 ERLLDLIKQPNVQSRPQKEGTESEQFWNLLGGKSEYPSQKISREAESDPHLFSCTFSGGN 242
           ER+LDLIK PN  ++ QKEG+ESEQFW LLGGKSEYPSQKI R+ ESDPHLFSCT++  +
Sbjct: 577 ERMLDLIK-PNEPTKAQKEGSESEQFWELLGGKSEYPSQKIKRDGESDPHLFSCTYTNES 636

Query: 243 LK 245
           LK
Sbjct: 637 LK 637

[Close]

BLAST of Carg23921-RA vs. TAIR 10
Match: AT2G41740.1 (villin 2 )

HSP 1 Score: 229.6 bits (584), Expect = 3.1e-60
Identity = 115/244 (47.13%), Postives = 156/244 (63.93%), Query Frame = 0


Query: 3   QVWRVSGNEKILIPTSDQSKFYSGDCYIFQYSY-PGDDKDEYLIGTWFGKQSVEEERATA 62
           +VW V+G  K  +P  D  K YSGDCY+  Y+Y  G+ KDEY +  WFGK+S+ E++ TA
Sbjct: 393 EVWYVNGKVKTPLPKEDIGKLYSGDCYLVLYTYHSGERKDEYFLSCWFGKKSIPEDQDTA 452

Query: 63  LSLVNKMVESLKFLPVQACLYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDET 122
           + L N M  SLK  PVQ  +YEG EP QF ++FQ  VV KGGLS GYK+ + E E  DET
Sbjct: 453 IRLANTMSNSLKGRPVQGRIYEGKEPPQFVALFQPMVVLKGGLSSGYKSSMGESESTDET 512

Query: 123 YQEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQEL 182
           Y  + IAL +V G+G  N +A+QV+ VA+SLNS  C++L S +S+F W G+ +  +  EL
Sbjct: 513 YTPESIALVQVSGTGVHNNKAVQVETVATSLNSYECFLLQSGTSMFLWHGNQSTHEQLEL 572

Query: 183 VERLLDLIKQPNVQSRPQKEGTESEQFWNLLGGKSEYPSQKISREAESDPHLFSCTFSGG 242
             ++ + +K P +  +  KEGTES  FW  LGGK  + S+K S E   DPHLFS  F+ G
Sbjct: 573 ATKVAEFLK-PGITLKHAKEGTESSTFWFALGGKQNFTSKKASSETIRDPHLFSFAFNRG 632

Query: 243 NLKV 246
             +V
Sbjct: 633 KFQV 635

[Close]

BLAST of Carg23921-RA vs. TAIR 10
Match: AT2G29890.2 (villin-like 1 )

HSP 1 Score: 227.6 bits (579), Expect = 1.2e-59
Identity = 112/240 (46.67%), Postives = 164/240 (68.33%), Query Frame = 0


Query: 3   QVWRVSGNEKILIPTSDQSKFYSGDCYIFQYSYPGDDKDEYLIGTWFGKQSVEEERATAL 62
           +VWRV G++  L+   DQ+K ++GDCY+ QY Y   ++ E+L+  W G +S++++RA A+
Sbjct: 392 KVWRVDGDDVSLLSIPDQTKLFTGDCYLVQYKYTYKERTEHLLYVWIGCESIQQDRADAI 451

Query: 63  SLVNKMVESLKFLPVQACLYEGNEPIQFYSIFQSFVVFKGGLSKGYKNYVAEKEIQDETY 122
           +  + +V + K   V   +Y+GNEP +F+ +FQS VVFKGGLS+ YK  +AEKE   E Y
Sbjct: 452 TNASAIVGTTKGESVLCHIYQGNEPSRFFPMFQSLVVFKGGLSRRYKVLLAEKEKIGEEY 511

Query: 123 QEDGIALFRVQGSGPENMQAIQVDPVASSLNSSYCYILNSSSSVFTWSGSLTNSDNQELV 182
            E+  +LFRV G+ P NMQAIQV+ VA+SLNSSY YIL   +S FTW G L++  + E++
Sbjct: 512 NENKASLFRVVGTSPRNMQAIQVNLVATSLNSSYSYILQYGASAFTWIGKLSSDSDHEVL 571

Query: 183 ERLLDLIKQPNVQSRPQKEGTESEQFWNLLGGKSEYPSQKISREAESDPHLFSCTFSGGN 242
           +R+L  +   + Q    +EG E++ FWNLLGGKSEYP +K  R+   +PHLF+C+ S GN
Sbjct: 572 DRMLYFL-DTSCQPIYIREGNETDTFWNLLGGKSEYPKEKEMRKQIEEPHLFTCSCSSGN 630

The following BLAST results are available for this feature:

BLAST of Carg23921-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7029831.1	3.6e-151	100.00	Villin-4, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022997656.1	2.0e-133	98.77	villin-4-like isoform X1 [Cucurbita maxima] >XP_022997657.1 villin-4-like isofor...	[more]
KAG6598881.1	2.0e-133	99.59	Villin-4, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022929553.1	2.6e-133	99.18	villin-4-like isoform X1 [Cucurbita moschata] >XP_022929554.1 villin-4-like isof...	[more]
XP_023521363.1	5.7e-133	98.77	villin-4-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023545663.1 villin-4-l...	[more]

BLAST of Carg23921-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O65570	8.6e-100	70.78	Villin-4 OS=Arabidopsis thaliana OX=3702 GN=VLN4 PE=1 SV=1	[more]
Q0J716	4.7e-98	70.78	Villin-5 OS=Oryza sativa subsp. japonica OX=39947 GN=VLN5 PE=3 SV=1	[more]
Q9LVC6	4.4e-96	66.94	Villin-5 OS=Arabidopsis thaliana OX=3702 GN=VLN5 PE=2 SV=1	[more]
Q67U26	2.3e-92	66.67	Villin-3 OS=Oryza sativa subsp. japonica OX=39947 GN=VLN3 PE=2 SV=2	[more]
B8ATT7	1.5e-88	64.03	Villin-4 OS=Oryza sativa subsp. indica OX=39946 GN=VLN4 PE=3 SV=1	[more]

BLAST of Carg23921-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K5Q4	9.5e-134	98.77	villin-4-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492524 PE=4 SV=1	[more]
A0A6J1EP34	1.2e-133	99.18	villin-4-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436105 PE=4 SV=1	[more]
A0A6J1KAG3	8.9e-132	98.35	villin-4-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111492524 PE=4 SV=1	[more]
A0A6J1EUQ1	1.2e-131	98.77	villin-4-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111436105 PE=4 SV=1	[more]
A0A6J1FCF4	2.7e-128	91.70	villin-4 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111444348 PE=3 SV=1	[more]

BLAST of Carg23921-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G30160.1	6.1e-101	70.78	villin 4	[more]
AT4G30160.2	6.1e-101	70.04	villin 4	[more]
AT5G57320.1	3.1e-97	66.94	villin, putative	[more]
AT2G41740.1	3.1e-60	47.13	villin 2	[more]
AT2G29890.2	1.2e-59	46.67	villin-like 1	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007122	Villin/Gelsolin	PRINTS	PR00597	GELSOLIN	coord: 201..220 score: 54.09 coord: 19..35 score: 47.59 coord: 77..97 score: 38.96 coord: 140..160 score: 45.24 coord: 44..62 score: 32.78
IPR007122	Villin/Gelsolin	SMART	SM00262	VILL_6	coord: 127..215 e-value: 2.5E-15 score: 66.9 coord: 1..97 e-value: 2.6E-19 score: 80.2
IPR007122	Villin/Gelsolin	PANTHER	PTHR11977	VILLIN	coord: 3..249
IPR029006	ADF-H/Gelsolin-like domain superfamily	GENE3D	3.40.20.10	Severin	coord: 131..222 e-value: 3.2E-24 score: 86.8
IPR029006	ADF-H/Gelsolin-like domain superfamily	GENE3D	3.40.20.10	Severin	coord: 1..130 e-value: 1.6E-27 score: 98.0
IPR007123	Gelsolin-like domain	PFAM	PF00626	Gelsolin	coord: 15..90 e-value: 2.1E-5 score: 24.3
None	No IPR available	PANTHER	PTHR11977:SF51	VILLIN-4	coord: 3..249
None	No IPR available	CDD	cd11293	gelsolin_S4_like	coord: 2..94 e-value: 1.05325E-37 score: 126.617
None	No IPR available	CDD	cd11288	gelsolin_S5_like	coord: 125..217 e-value: 1.19554E-32 score: 113.48
None	No IPR available	SUPERFAMILY	55753	Actin depolymerizing proteins	coord: 119..231
None	No IPR available	SUPERFAMILY	55753	Actin depolymerizing proteins	coord: 3..136

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg23921	Carg23921	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg23921-RA:7	Carg23921-RA:7	exon
Carg23921-RA:6	Carg23921-RA:6	exon
Carg23921-RA:5	Carg23921-RA:5	exon
Carg23921-RA:4	Carg23921-RA:4	exon
Carg23921-RA:3	Carg23921-RA:3	exon
Carg23921-RA:2	Carg23921-RA:2	exon
Carg23921-RA:1	Carg23921-RA:1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg23921-RA:cds	Carg23921-RA:cds_7	CDS
Carg23921-RA:cds	Carg23921-RA:cds_6	CDS
Carg23921-RA:cds	Carg23921-RA:cds_5	CDS
Carg23921-RA:cds	Carg23921-RA:cds_4	CDS
Carg23921-RA:cds	Carg23921-RA:cds_3	CDS
Carg23921-RA:cds	Carg23921-RA:cds_2	CDS
Carg23921-RA:cds	Carg23921-RA:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg23921-RA	Carg23921-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051014	actin filament severing
biological_process	GO:0051693	actin filament capping
molecular_function	GO:0051015	actin filament binding