Carg23718-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg23718-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Carbonic anhydrase
Location	Carg_Chr12: 6391636 .. 6393136 (-)
Sequence length	801
RNA-Seq Expression	Carg23718-RA
Synteny	Carg23718-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGAAGCTCTCTTTTCATGCGTTGTTTTGCACATTTTTGTGTGCCTTCCTTTTGGTTTCATGGCCTGCAATGTCTCAAGAAGTTGGTAGGGAACCTATTGTGTCATATACTTTATTATGATATGAGCTTTGTTTGTATAATATTTAAATGTTTGTTTGATATTGCATGCAGAGGATCAGAAGGAGTTTGATTATAATGAAGAGGGAAAGAGAGGGCCTTCCCATTGGGGAGATCTTAAGAGAGAGTGGCATAGCTGCAAGGCTGGGCTAATGCAATCTCCCATTGATCTATTGCACGAGCGAGTTCGAATTGTACCCCATTTTACGAACCTCAAGACCGAGTATAAGCCTTCTAATGCAACTCTTAAGAACCGAGGCCATGATATTATGGTAATTGTTGTAATATCAACTTTTGCTTATAATTATGTTTAAAAGAATACCCATTTGAGTATGGATTGTGATGGTTATTGCAGTTGAAATGGGGGTGAAGGGGCTGGCTATATGGAAGTTAATGGAACTCGGTACTTTCTTAAGCAATTCCATTGGCATTCGCCCTCTGAGCATACAATCAATGGCAGAAGATTTGCTTTAGAAGCCCATTTGGTGCACACCAAAAGTTCAACAGGAAGTGTTTGCTGTCATTGGTGTTCTCTACAAAATTGGACGGCCTGATTATTTCTTGTCTAAGGTAAATGTTCAAATATAATTTTTGAAGATGAGAAATGATTAAAATTGTTATGTTCTAAATTTAACACTTTTTTTTCTTAGATGAAAGAGTATTTGGAAGAAGTTTGTGATACAAAAGAAGATAAAGCCATTGATTTGGCTGATCCGTCCCAATTAGAGATGCTAAGCAGTTTATATTATCGATATATTGGCTCTCTAACCGTTCCACCGTGTTCTCAAAATGTGCTTTGGACCATAGTCAGAAAGGTATTTTTTTTTTCTCAAGTTAGATCCTCCTATATATATTCATATATTTATGTTTCACAAGTACTACATTTTTTTTAATGCTTAAATAATTTTTATACGTTTTTTTTCAGGTTAAGACGGTTTCACCTGAACAAGTGAAATTGCTTCGAGTGGCTGTTCATGATGTAAGCAATATAATTTTATGTCCATTGAAAATTTAGTTTTTAAATGAATTTTGAAATTTTTATTTAATCCTCTCGAATTTCTATTCAAGTATTTTCATATTCGTTGCTTTGATTTGTATTCAGATATATTTATTTTTTTAATTTTGTAATTAGGTTTTACGTATTTAGTTCATTTATCGTAGCATTATAATTTTCAAAAACTCTATGATCTGATTTAGAACGGATTAAATTACTTGCTCCTTATTAGCAATATTATATTCTTAAAAAAAAAGACAAAAACTAAAATTTTATGATATATAATATGGTTTTAATTGGTTTATATATATGCAGGACTCAGACACTAATGCGAGGCCTTTACAACCGCTTAATGATCGAAAGATTCAACTCCGAGTCAAATCATGA

mRNA sequence

ATGGAGAAGCTCTCTTTTCATGCGTTGTTTTGCACATTTTTGTGTGCCTTCCTTTTGGTTTCATGGCCTGCAATGTCTCAAGAAGTTGAGGATCAGAAGGAGTTTGATTATAATGAAGAGGGAAAGAGAGGGCCTTCCCATTGGGGAGATCTTAAGAGAGAGTGGCATAGCTGCAAGGCTGGGCTAATGCAATCTCCCATTGATCTATTGCACGAGCGAGTTCGAATTGTACCCCATTTTACGAACCTCAAGACCGAGTATAAGCCTTCTAATGCAACTCTTAAGAACCGAGGCCATGATATTATGAATACCCATTTGAGTATGGATTGTGATGGGGCTGGCTATATGGAAGTTAATGGAACTCGGTACTTTCTTAAGCAATTCCATTGGCATTCGCCCTCTGAGCATACAATCAATGGCAGAAGATTTGCTTTAGAAGCCCATTTGGAAGTGTTTGCTGTCATTGGTGTTCTCTACAAAATTGGACGGCCTGATTATTTCTTGTCTAAGATGAAAGAGTATTTGGAAGAAGTTTGTGATACAAAAGAAGATAAAGCCATTGATTTGGCTGATCCGTCCCAATTAGAGATGCTAAGCAGTTTATATTATCGATATATTGGCTCTCTAACCGTTCCACCGTGTTCTCAAAATGTGCTTTGGACCATAGTCAGAAAGGTTAAGACGGTTTCACCTGAACAAGTGAAATTGCTTCGAGTGGCTGTTCATGATGACTCAGACACTAATGCGAGGCCTTTACAACCGCTTAATGATCGAAAGATTCAACTCCGAGTCAAATCATGA

Coding sequence (CDS)

ATGGAGAAGCTCTCTTTTCATGCGTTGTTTTGCACATTTTTGTGTGCCTTCCTTTTGGTTTCATGGCCTGCAATGTCTCAAGAAGTTGAGGATCAGAAGGAGTTTGATTATAATGAAGAGGGAAAGAGAGGGCCTTCCCATTGGGGAGATCTTAAGAGAGAGTGGCATAGCTGCAAGGCTGGGCTAATGCAATCTCCCATTGATCTATTGCACGAGCGAGTTCGAATTGTACCCCATTTTACGAACCTCAAGACCGAGTATAAGCCTTCTAATGCAACTCTTAAGAACCGAGGCCATGATATTATGAATACCCATTTGAGTATGGATTGTGATGGGGCTGGCTATATGGAAGTTAATGGAACTCGGTACTTTCTTAAGCAATTCCATTGGCATTCGCCCTCTGAGCATACAATCAATGGCAGAAGATTTGCTTTAGAAGCCCATTTGGAAGTGTTTGCTGTCATTGGTGTTCTCTACAAAATTGGACGGCCTGATTATTTCTTGTCTAAGATGAAAGAGTATTTGGAAGAAGTTTGTGATACAAAAGAAGATAAAGCCATTGATTTGGCTGATCCGTCCCAATTAGAGATGCTAAGCAGTTTATATTATCGATATATTGGCTCTCTAACCGTTCCACCGTGTTCTCAAAATGTGCTTTGGACCATAGTCAGAAAGGTTAAGACGGTTTCACCTGAACAAGTGAAATTGCTTCGAGTGGCTGTTCATGATGACTCAGACACTAATGCGAGGCCTTTACAACCGCTTAATGATCGAAAGATTCAACTCCGAGTCAAATCATGA

Protein sequence

MEKLSFHALFCTFLCAFLLVSWPAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKAGLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMNTHLSMDCDGAGYMEVNGTRYFLKQFHWHSPSEHTINGRRFALEAHLEVFAVIGVLYKIGRPDYFLSKMKEYLEEVCDTKEDKAIDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKVKTVSPEQVKLLRVAVHDDSDTNARPLQPLNDRKIQLRVKS

Homology

[Close]

BLAST of Carg23718-RA vs. NCBI nr
Match: KAG7020783.1 (Alpha carbonic anhydrase 7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 553.9 bits (1426), Expect = 7.5e-154
Identity = 266/266 (100.00%), Postives = 266/266 (100.00%), Query Frame = 0


Query: 1   MEKLSFHALFCTFLCAFLLVSWPAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60
           MEKLSFHALFCTFLCAFLLVSWPAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA
Sbjct: 1   MEKLSFHALFCTFLCAFLLVSWPAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60

Query: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMNTHLSMDCDGAGYMEVNG 120
           GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMNTHLSMDCDGAGYMEVNG
Sbjct: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMNTHLSMDCDGAGYMEVNG 120

Query: 121 TRYFLKQFHWHSPSEHTINGRRFALEAHLEVFAVIGVLYKIGRPDYFLSKMKEYLEEVCD 180
           TRYFLKQFHWHSPSEHTINGRRFALEAHLEVFAVIGVLYKIGRPDYFLSKMKEYLEEVCD
Sbjct: 121 TRYFLKQFHWHSPSEHTINGRRFALEAHLEVFAVIGVLYKIGRPDYFLSKMKEYLEEVCD 180

Query: 181 TKEDKAIDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKVKTVSPEQVKLLRVA 240
           TKEDKAIDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKVKTVSPEQVKLLRVA
Sbjct: 181 TKEDKAIDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKVKTVSPEQVKLLRVA 240

Query: 241 VHDDSDTNARPLQPLNDRKIQLRVKS 267
           VHDDSDTNARPLQPLNDRKIQLRVKS
Sbjct: 241 VHDDSDTNARPLQPLNDRKIQLRVKS 266

[Close]

BLAST of Carg23718-RA vs. NCBI nr
Match: KAG7020784.1 (Alpha carbonic anhydrase 7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 510.0 bits (1312), Expect = 1.2e-140
Identity = 253/272 (93.01%), Postives = 255/272 (93.75%), Query Frame = 0


Query: 1   MEKLSFHALFCTFLCAFLLVSWPAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60
           MEKLSFHALFCTFLCAFLLVSWPAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA
Sbjct: 1   MEKLSFHALFCTFLCAFLLVSWPAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60

Query: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMNTHLSMDCDGAGYMEVNG 120
           GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIM        +GAGYMEVNG
Sbjct: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMLKW----GEGAGYMEVNG 120

Query: 121 TRYFLKQFHWHSPSEHTINGRRFALEAHL------EVFAVIGVLYKIGRPDYFLSKMKEY 180
           TRYFLKQFHWHSPSEHTINGRRFALEAHL         AVIGVLYKIGRPDYFLSKMKEY
Sbjct: 121 TRYFLKQFHWHSPSEHTINGRRFALEAHLVHQSSTGSVAVIGVLYKIGRPDYFLSKMKEY 180

Query: 181 LEEVCDTKEDKAIDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKVKTVSPEQV 240
           LEEVCDTKEDKAIDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKVKTVSPEQV
Sbjct: 181 LEEVCDTKEDKAIDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKVKTVSPEQV 240

Query: 241 KLLRVAVHDDSDTNARPLQPLNDRKIQLRVKS 267
           KLLRVAVHDDSDTNARPLQPLNDRKIQLR+KS
Sbjct: 241 KLLRVAVHDDSDTNARPLQPLNDRKIQLRIKS 268

[Close]

BLAST of Carg23718-RA vs. NCBI nr
Match: KAG6583573.1 (Alpha carbonic anhydrase 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 509.6 bits (1311), Expect = 1.6e-140
Identity = 253/272 (93.01%), Postives = 255/272 (93.75%), Query Frame = 0


Query: 1   MEKLSFHALFCTFLCAFLLVSWPAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60
           MEKLSFHALFCTFLCAFL+VSWPAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA
Sbjct: 1   MEKLSFHALFCTFLCAFLMVSWPAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60

Query: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMNTHLSMDCDGAGYMEVNG 120
           GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIM        +GAGYMEVNG
Sbjct: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMLKW----GEGAGYMEVNG 120

Query: 121 TRYFLKQFHWHSPSEHTINGRRFALEAHL------EVFAVIGVLYKIGRPDYFLSKMKEY 180
           TRYFLKQFHWHSPSEHTINGRRFALEAHL         AVIGVLYKIGRPDYFLSKMKEY
Sbjct: 121 TRYFLKQFHWHSPSEHTINGRRFALEAHLVHQSSTGSVAVIGVLYKIGRPDYFLSKMKEY 180

Query: 181 LEEVCDTKEDKAIDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKVKTVSPEQV 240
           LEEVCDTKEDKAIDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKVKTVSPEQV
Sbjct: 181 LEEVCDTKEDKAIDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKVKTVSPEQV 240

Query: 241 KLLRVAVHDDSDTNARPLQPLNDRKIQLRVKS 267
           KLLRVAVHDDSDTNARPLQPLNDRKIQLRVKS
Sbjct: 241 KLLRVAVHDDSDTNARPLQPLNDRKIQLRVKS 268

[Close]

BLAST of Carg23718-RA vs. NCBI nr
Match: KAG6583574.1 (Alpha carbonic anhydrase 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 509.2 bits (1310), Expect = 2.1e-140
Identity = 252/272 (92.65%), Postives = 255/272 (93.75%), Query Frame = 0


Query: 1   MEKLSFHALFCTFLCAFLLVSWPAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60
           MEKLSFHALFCTFLCAFL+VSWPAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA
Sbjct: 1   MEKLSFHALFCTFLCAFLMVSWPAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60

Query: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMNTHLSMDCDGAGYMEVNG 120
           GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIM        +GAGYMEVNG
Sbjct: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMLKW----GEGAGYMEVNG 120

Query: 121 TRYFLKQFHWHSPSEHTINGRRFALEAHL------EVFAVIGVLYKIGRPDYFLSKMKEY 180
           TRYFLKQFHWHSPSEHTINGRRFALEAHL         AVIGVLYKIGRPDYFLSKMKEY
Sbjct: 121 TRYFLKQFHWHSPSEHTINGRRFALEAHLVHQSSTGSVAVIGVLYKIGRPDYFLSKMKEY 180

Query: 181 LEEVCDTKEDKAIDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKVKTVSPEQV 240
           LEEVCDTKEDKAIDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKVKTVSPEQV
Sbjct: 181 LEEVCDTKEDKAIDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKVKTVSPEQV 240

Query: 241 KLLRVAVHDDSDTNARPLQPLNDRKIQLRVKS 267
           KLLRVAVHDDSDTNARPLQPLNDRKIQLR+KS
Sbjct: 241 KLLRVAVHDDSDTNARPLQPLNDRKIQLRIKS 268

[Close]

BLAST of Carg23718-RA vs. NCBI nr
Match: XP_022964926.1 (alpha carbonic anhydrase 7-like [Cucurbita moschata])

HSP 1 Score: 500.7 bits (1288), Expect = 7.5e-138
Identity = 248/272 (91.18%), Postives = 253/272 (93.01%), Query Frame = 0


Query: 1   MEKLSFHALFCTFLCAFLLVSWPAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60
           MEKLSFHALFCTFLCAFL+VSWPAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA
Sbjct: 1   MEKLSFHALFCTFLCAFLMVSWPAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60

Query: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMNTHLSMDCDGAGYMEVNG 120
           GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIM        +GAGYMEVNG
Sbjct: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMLKW----GEGAGYMEVNG 120

Query: 121 TRYFLKQFHWHSPSEHTINGRRFALEAHL------EVFAVIGVLYKIGRPDYFLSKMKEY 180
           TRYFLKQFHWHSPSEHTINGRRFALEAHL         AVIGVLYKIGRPDYFLSKMKEY
Sbjct: 121 TRYFLKQFHWHSPSEHTINGRRFALEAHLVHQSPTGSVAVIGVLYKIGRPDYFLSKMKEY 180

Query: 181 LEEVCDTKEDKAIDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKVKTVSPEQV 240
           LEEVCDTKEDKAIDL  PSQLEMLSSLYY+YIGSLTVPPCSQNVLWTIVRKVKTVSPEQV
Sbjct: 181 LEEVCDTKEDKAIDLGHPSQLEMLSSLYYQYIGSLTVPPCSQNVLWTIVRKVKTVSPEQV 240

Query: 241 KLLRVAVHDDSDTNARPLQPLNDRKIQLRVKS 267
           KLLRVAVHDDSDTNARPLQPL+DR+IQLRVKS
Sbjct: 241 KLLRVAVHDDSDTNARPLQPLHDRRIQLRVKS 268

[Close]

BLAST of Carg23718-RA vs. ExPASy Swiss-Prot
Match: Q8L817 (Alpha carbonic anhydrase 7 OS=Arabidopsis thaliana OX=3702 GN=ACA7 PE=2 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 1.0e-73
Identity = 143/265 (53.96%), Postives = 188/265 (70.94%), Query Frame = 0


Query: 6   FHALFCTFLCAFLLVSWPAMSQ-EVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKAGLMQ 65
           F ALF  F    + +S  A S  EVED++EF+Y +  ++GP  WG+LK EW  C  G MQ
Sbjct: 12  FVALFSIF--TIVSISSAASSHGEVEDEREFNYKKNDEKGPERWGELKPEWEMCGKGEMQ 71

Query: 66  SPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMNTHLSMDCDGAGYMEVNGTRYF 125
           SPIDL++ERV IV H   L  +Y PSNATLKNRGHDIM   L  + DGAG +++NG  Y 
Sbjct: 72  SPIDLMNERVNIVSHLGRLNRDYNPSNATLKNRGHDIM---LKFE-DGAGTIKINGFEYE 131

Query: 126 LKQFHWHSPSEHTINGRRFALEAHL------EVFAVIGVLYKIGRPDYFLSKMKEYLEEV 185
           L+Q HWHSPSEHTINGRRFALE H+         AV+ VLYKIGR D F+  +++ LE +
Sbjct: 132 LQQLHWHSPSEHTINGRRFALELHMVHEGRNRRMAVVTVLYKIGRADTFIRSLEKELEGI 191

Query: 186 CDTKE-DKAIDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKVKTVSPEQVKLL 245
            + +E +K + + DP+++++ S  YYRY GSLT PPC+QNV W++VRKV+TV+ +QVKLL
Sbjct: 192 AEMEEAEKNVGMIDPTKIKIGSRKYYRYTGSLTTPPCTQNVTWSVVRKVRTVTRKQVKLL 251

Query: 246 RVAVHDDSDTNARPLQPLNDRKIQL 263
           RVAVHDD+++NARP+QP N R + L
Sbjct: 252 RVAVHDDANSNARPVQPTNKRIVHL 270

[Close]

BLAST of Carg23718-RA vs. ExPASy Swiss-Prot
Match: Q9FM99 (Alpha carbonic anhydrase 8 OS=Arabidopsis thaliana OX=3702 GN=ACA8 PE=3 SV=3)

HSP 1 Score: 248.8 bits (634), Expect = 6.8e-65
Identity = 125/247 (50.61%), Postives = 166/247 (67.21%), Query Frame = 0


Query: 23  PAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKAGLMQSPIDLLHERVRIVPHFTN 82
           PA   EVED+ EF Y  +G +GP+ WG L  EW  C  G MQSPIDL  + V +   F  
Sbjct: 128 PAPGGEVEDETEFSYETKGNKGPAKWGTLDAEWKMCGIGKMQSPIDLRDKNVVVSNKFGL 187

Query: 83  LKTEYKPSNATLKNRGHDIMNTHLSMDCDGAGYMEVNGTRYFLKQFHWHSPSEHTINGRR 142
           L+++Y PSN T+KNRGHDIM         G G + + GTRY L+Q HWHSPSEHTING+R
Sbjct: 188 LRSQYLPSNTTIKNRGHDIM-LKFKGGNKGIG-VTIRGTRYQLQQLHWHSPSEHTINGKR 247

Query: 143 FALEAHL------EVFAVIGVLYKIGRPDYFLSKMKEYLEEVCDT-KEDKAIDLADPSQL 202
           FALE HL      + +AV+  LY +G  D FL  +++ L+++ DT   ++ + + DP +L
Sbjct: 248 FALEEHLVHESKDKRYAVVAFLYNLGASDPFLFSLEKQLKKITDTHASEEHVGIIDPKKL 307

Query: 203 EMLSSLYYRYIGSLTVPPCSQNVLWTIVRKVKTVSPEQVKLLRVAVHDDSDTNARPLQPL 262
              S  YYRY GSLT PPCS+NV+W++ ++++TVS +QVKLLRVAVHD SD+NARPLQ +
Sbjct: 308 SFESKHYYRYSGSLTAPPCSENVIWSVSKEIRTVSSKQVKLLRVAVHDASDSNARPLQAV 367

[Close]

BLAST of Carg23718-RA vs. ExPASy Swiss-Prot
Match: F4HUC4 (Alpha carbonic anhydrase 5 OS=Arabidopsis thaliana OX=3702 GN=ACA5 PE=3 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 1.7e-63
Identity = 123/266 (46.24%), Postives = 176/266 (66.17%), Query Frame = 0


Query: 7   HALFCTFLCAFLLVSWPAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKAGLMQSP 66
           +  F  F+   ++ S P    EVED+ +F+Y ++G++GP +WG LK EW  C  G MQSP
Sbjct: 8   YVFFLIFISITIVSSSPDHG-EVEDETQFNYEKKGEKGPENWGRLKPEWAMCGKGNMQSP 67

Query: 67  IDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMNTHLSMDCDGAGY-MEVNGTRYFL 126
           IDL  +RV I  +   L+++Y PSNAT+KNRGHDIM   +  +   AG  + +NGT Y L
Sbjct: 68  IDLTDKRVLIDHNLGYLRSQYLPSNATIKNRGHDIM---MKFEGGNAGLGITINGTEYKL 127

Query: 127 KQFHWHSPSEHTINGRRFALEAHL------EVFAVIGVLYKIGRPDYFLSKMKEYLEEVC 186
           +Q HWHSPSEHT+NG+RF LE H+         AV+   YK+G+PDYFL  ++ YL+ + 
Sbjct: 128 QQIHWHSPSEHTLNGKRFVLEEHMVHQSKDGRNAVVAFFYKLGKPDYFLLTLERYLKRIT 187

Query: 187 DTKEDKA-IDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKVKTVSPEQVKLLR 246
           DT E +  +++  P      S  YYR+IGSLT PPCS+NV+WTI ++++TV+ +Q+ +LR
Sbjct: 188 DTHESQEFVEMVHPRTFGFESKHYYRFIGSLTTPPCSENVIWTISKEMRTVTLKQLIMLR 247

Query: 247 VAVHDDSDTNARPLQPLNDRKIQLRV 265
           V VHD S++NARPLQ  N+R + L +
Sbjct: 248 VTVHDQSNSNARPLQRKNERPVALYI 269

[Close]

BLAST of Carg23718-RA vs. ExPASy Swiss-Prot
Match: Q84UV8 (Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3 OS=Nicotiana langsdorffii x Nicotiana sanderae OX=164110 GN=NEC3 PE=1 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 2.2e-63
Identity = 128/260 (49.23%), Postives = 177/260 (68.08%), Query Frame = 0


Query: 9   LFCTFLCAFLLVSWPAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKAGLMQSPID 68
           LF +FL  FL   + A S EV+D+ EF Y+E+ + GP++WG+++ +W  C +G +QSPID
Sbjct: 10  LFISFL--FLSSVFLARSGEVDDESEFSYDEKSENGPANWGNIRPDWKEC-SGKLQSPID 69

Query: 69  LLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMNTHLSMDCDGAGYMEVNGTRYFLKQF 128
           +   R  +V +   L+ +YKPSNATL NRGHDIM     +  D  GY+++N T+Y LKQ 
Sbjct: 70  IFDLRAEVVSNLRILQKDYKPSNATLLNRGHDIM-----LRLDDGGYLKINETQYQLKQL 129

Query: 129 HWHSPSEHTINGRRFALEAHL------EVFAVIGVLYKIGR-PDYFLSKMKEYLEEVCDT 188
           HWH+PSEHTING RF LEAHL        F VIG++Y+IG  PD FLS ++  L+   + 
Sbjct: 130 HWHTPSEHTINGERFNLEAHLVHESNNGKFVVIGIVYEIGLWPDPFLSMIENDLKVPANK 189

Query: 189 KE-DKAIDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKVKTVSPEQVKLLRVA 248
           K  ++ I + DP+Q+++    Y+RYIGSLT PPC++ V+W I RKVKTV+  Q+KLL+ A
Sbjct: 190 KGIERGIGIIDPNQIKLDGKKYFRYIGSLTTPPCTEGVVWIIDRKVKTVTRRQIKLLQEA 249

Query: 249 VHDDSDTNARPLQPLNDRKI 261
           VHD  +TNARP QP N+R I
Sbjct: 250 VHDGFETNARPTQPENERYI 261

[Close]

BLAST of Carg23718-RA vs. ExPASy Swiss-Prot
Match: F4IHR4 (Alpha carbonic anhydrase 2 OS=Arabidopsis thaliana OX=3702 GN=ACA2 PE=2 SV=2)

HSP 1 Score: 229.2 bits (583), Expect = 5.5e-59
Identity = 118/239 (49.37%), Postives = 153/239 (64.02%), Query Frame = 0


Query: 1   MEKLSFHALFCTFLCAFL----LVSWPAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWH 60
           M+K+S        L +F+     +S     +EVED+ EF Y    + GP+ WG L+ EW 
Sbjct: 1   MDKISIRCFIFLVLTSFVTTVSCLSAATDYREVEDEHEFSYEWNQENGPAKWGKLRPEWK 60

Query: 61  SCKAGLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMNTHLSMDCDGAGYM 120
            C  G MQSPIDL+++RVR+V H   L   YKP NATLKNRGHD+M   L    +G+G +
Sbjct: 61  MCGKGEMQSPIDLMNKRVRLVTHLKKLTRHYKPCNATLKNRGHDMM---LKFGEEGSGSI 120

Query: 121 EVNGTRYFLKQFHWHSPSEHTINGRRFALEAHL------EVFAVIGVLYKIGRPDYFLSK 180
            VNGT Y L Q HWHSPSEHT+NGRRFALE H+         AV+ VLYKIGRPD FL  
Sbjct: 121 TVNGTEYKLLQLHWHSPSEHTMNGRRFALELHMVHENINGSLAVVTVLYKIGRPDSFLGL 180

Query: 181 MKEYLEEVCDTKE-DKAIDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKVKT 229
           ++  L  + D  E +K +D+ DP  +++ S  +YRYIGSLT PPC+QNV+WT+V+KV T
Sbjct: 181 LENKLSAITDQNEAEKYVDVIDPRDIKIGSRKFYRYIGSLTTPPCTQNVIWTVVKKVNT 236

[Close]

BLAST of Carg23718-RA vs. ExPASy TrEMBL
Match: A0A6J1HKB2 (Carbonic anhydrase OS=Cucurbita moschata OX=3662 GN=LOC111464883 PE=3 SV=1)

HSP 1 Score: 500.7 bits (1288), Expect = 3.6e-138
Identity = 248/272 (91.18%), Postives = 253/272 (93.01%), Query Frame = 0


Query: 1   MEKLSFHALFCTFLCAFLLVSWPAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60
           MEKLSFHALFCTFLCAFL+VSWPAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA
Sbjct: 1   MEKLSFHALFCTFLCAFLMVSWPAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60

Query: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMNTHLSMDCDGAGYMEVNG 120
           GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIM        +GAGYMEVNG
Sbjct: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMLKW----GEGAGYMEVNG 120

Query: 121 TRYFLKQFHWHSPSEHTINGRRFALEAHL------EVFAVIGVLYKIGRPDYFLSKMKEY 180
           TRYFLKQFHWHSPSEHTINGRRFALEAHL         AVIGVLYKIGRPDYFLSKMKEY
Sbjct: 121 TRYFLKQFHWHSPSEHTINGRRFALEAHLVHQSPTGSVAVIGVLYKIGRPDYFLSKMKEY 180

Query: 181 LEEVCDTKEDKAIDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKVKTVSPEQV 240
           LEEVCDTKEDKAIDL  PSQLEMLSSLYY+YIGSLTVPPCSQNVLWTIVRKVKTVSPEQV
Sbjct: 181 LEEVCDTKEDKAIDLGHPSQLEMLSSLYYQYIGSLTVPPCSQNVLWTIVRKVKTVSPEQV 240

Query: 241 KLLRVAVHDDSDTNARPLQPLNDRKIQLRVKS 267
           KLLRVAVHDDSDTNARPLQPL+DR+IQLRVKS
Sbjct: 241 KLLRVAVHDDSDTNARPLQPLHDRRIQLRVKS 268

[Close]

BLAST of Carg23718-RA vs. ExPASy TrEMBL
Match: A0A6J1HYT6 (Carbonic anhydrase OS=Cucurbita maxima OX=3661 GN=LOC111469328 PE=3 SV=1)

HSP 1 Score: 495.0 bits (1273), Expect = 2.0e-136
Identity = 245/272 (90.07%), Postives = 252/272 (92.65%), Query Frame = 0


Query: 1   MEKLSFHALFCTFLCAFLLVSWPAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60
           M+KLSFHALFCTFLCAFLLVSW AMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA
Sbjct: 1   MKKLSFHALFCTFLCAFLLVSWRAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60

Query: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMNTHLSMDCDGAGYMEVNG 120
           GLMQSPIDLLHERVRIVPHFTNLKTEYKP NATLKNRGHDIM        +GAGYMEVNG
Sbjct: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPCNATLKNRGHDIMLKW----GEGAGYMEVNG 120

Query: 121 TRYFLKQFHWHSPSEHTINGRRFALEAHL------EVFAVIGVLYKIGRPDYFLSKMKEY 180
           TRYFLKQFHWHSPSEHTINGRRFALEAHL         AVIGVLYKIGRPDYFLSKM+EY
Sbjct: 121 TRYFLKQFHWHSPSEHTINGRRFALEAHLVHQSPTGSVAVIGVLYKIGRPDYFLSKMEEY 180

Query: 181 LEEVCDTKEDKAIDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKVKTVSPEQV 240
           LEEV DT+EDKAIDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRK++TVSPEQV
Sbjct: 181 LEEVSDTREDKAIDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKIRTVSPEQV 240

Query: 241 KLLRVAVHDDSDTNARPLQPLNDRKIQLRVKS 267
           KLLRVAVHDDSDTNARPLQPLNDRKIQLR+KS
Sbjct: 241 KLLRVAVHDDSDTNARPLQPLNDRKIQLRIKS 268

[Close]

BLAST of Carg23718-RA vs. ExPASy TrEMBL
Match: A0A6J1I3K8 (Carbonic anhydrase OS=Cucurbita maxima OX=3661 GN=LOC111469329 PE=3 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 7.6e-136
Identity = 245/272 (90.07%), Postives = 252/272 (92.65%), Query Frame = 0


Query: 1   MEKLSFHALFCTFLCAFLLVSWPAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60
           M+KLSFHALFCTFLCAFLLVSW AMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA
Sbjct: 1   MKKLSFHALFCTFLCAFLLVSWRAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60

Query: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMNTHLSMDCDGAGYMEVNG 120
           GLMQSPIDLLHERVRIVPHFTNLKTEYKP NATLKNRGHDIM        +GAGYMEVNG
Sbjct: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPCNATLKNRGHDIMLKW----GEGAGYMEVNG 120

Query: 121 TRYFLKQFHWHSPSEHTINGRRFALEAHL------EVFAVIGVLYKIGRPDYFLSKMKEY 180
           TRYFLKQFHWHSPSEHTINGRRFALEAHL         AVIGVLYKIGRPDYFLSKM+E+
Sbjct: 121 TRYFLKQFHWHSPSEHTINGRRFALEAHLVHQSPTGSVAVIGVLYKIGRPDYFLSKMEEH 180

Query: 181 LEEVCDTKEDKAIDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKVKTVSPEQV 240
           LEEV DT+EDKAIDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRK++TVSPEQV
Sbjct: 181 LEEVSDTQEDKAIDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKIRTVSPEQV 240

Query: 241 KLLRVAVHDDSDTNARPLQPLNDRKIQLRVKS 267
           KLLRVAVHDDSDTNARPLQPLNDRKIQLRVKS
Sbjct: 241 KLLRVAVHDDSDTNARPLQPLNDRKIQLRVKS 268

[Close]

BLAST of Carg23718-RA vs. ExPASy TrEMBL
Match: A0A6J1HME8 (Carbonic anhydrase OS=Cucurbita moschata OX=3662 GN=LOC111464898 PE=3 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 5.9e-120
Identity = 217/239 (90.79%), Postives = 220/239 (92.05%), Query Frame = 0


Query: 1   MEKLSFHALFCTFLCAFLLVSWPAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60
           MEKLSFHALFCTFLCAFL+VSWPAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA
Sbjct: 1   MEKLSFHALFCTFLCAFLMVSWPAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60

Query: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMNTHLSMDCDGAGYMEVNG 120
           GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIM        +GAGYMEVNG
Sbjct: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMLKW----GEGAGYMEVNG 120

Query: 121 TRYFLKQFHWHSPSEHTINGRRFALEAHL------EVFAVIGVLYKIGRPDYFLSKMKEY 180
           TRYFLKQFHWHSPSEHTINGRRFALEAHL         AVIGVLYKIGRPDYFLSKMKEY
Sbjct: 121 TRYFLKQFHWHSPSEHTINGRRFALEAHLVHQSSTGSVAVIGVLYKIGRPDYFLSKMKEY 180

Query: 181 LEEVCDTKEDKAIDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKVKTVSPEQ 234
           LEEVCDTKEDKAIDL DPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKV+TVS EQ
Sbjct: 181 LEEVCDTKEDKAIDLGDPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKVRTVSLEQ 235

[Close]

BLAST of Carg23718-RA vs. ExPASy TrEMBL
Match: A0A6J1KFK2 (Carbonic anhydrase OS=Cucurbita maxima OX=3661 GN=LOC111493412 PE=3 SV=1)

HSP 1 Score: 422.2 bits (1084), Expect = 1.6e-114
Identity = 205/272 (75.37%), Postives = 231/272 (84.93%), Query Frame = 0


Query: 1   MEKLSFHALFCTFLCAFLLVSWPAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKA 60
           MEKL FH +F  FLCAFLLVSWP M QEVEDQKEFDY+ EG RGP+HWGDLKREWH CK 
Sbjct: 1   MEKLPFHVIFGAFLCAFLLVSWPVMCQEVEDQKEFDYDAEGDRGPTHWGDLKREWHGCKT 60

Query: 61  GLMQSPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMNTHLSMDCDGAGYMEVNG 120
           GLMQSPIDLLH+RVRIV HFTNLK +YKPSNATLKNRGHDIM         GAG + VNG
Sbjct: 61  GLMQSPIDLLHKRVRIVSHFTNLKIDYKPSNATLKNRGHDIMLKW----SGGAGSINVNG 120

Query: 121 TRYFLKQFHWHSPSEHTINGRRFALEAHL------EVFAVIGVLYKIGRPDYFLSKMKEY 180
           TRYFL+Q HWHSPSEHTINGRRFALEAHL         AVIG+LY IGRPD+FLSK++EY
Sbjct: 121 TRYFLRQCHWHSPSEHTINGRRFALEAHLVHQSQTGKVAVIGLLYNIGRPDHFLSKLEEY 180

Query: 181 LEEVCDTKEDKAIDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKVKTVSPEQV 240
           LE++ +++E   +++ DPS L+M SSLYYRYIGSLTVPPCSQNVLWTIVR+++TV+PEQV
Sbjct: 181 LEDISNSREYDELNVIDPSLLKMQSSLYYRYIGSLTVPPCSQNVLWTIVREIRTVAPEQV 240

Query: 241 KLLRVAVHDDSDTNARPLQPLNDRKIQLRVKS 267
           KLLRVAVHDDSDTNARPLQPLNDRKIQLR+K+
Sbjct: 241 KLLRVAVHDDSDTNARPLQPLNDRKIQLRIKN 268

[Close]

BLAST of Carg23718-RA vs. TAIR 10
Match: AT1G08080.1 (alpha carbonic anhydrase 7 )

HSP 1 Score: 278.1 bits (710), Expect = 7.4e-75
Identity = 143/265 (53.96%), Postives = 188/265 (70.94%), Query Frame = 0


Query: 6   FHALFCTFLCAFLLVSWPAMSQ-EVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKAGLMQ 65
           F ALF  F    + +S  A S  EVED++EF+Y +  ++GP  WG+LK EW  C  G MQ
Sbjct: 12  FVALFSIF--TIVSISSAASSHGEVEDEREFNYKKNDEKGPERWGELKPEWEMCGKGEMQ 71

Query: 66  SPIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMNTHLSMDCDGAGYMEVNGTRYF 125
           SPIDL++ERV IV H   L  +Y PSNATLKNRGHDIM   L  + DGAG +++NG  Y 
Sbjct: 72  SPIDLMNERVNIVSHLGRLNRDYNPSNATLKNRGHDIM---LKFE-DGAGTIKINGFEYE 131

Query: 126 LKQFHWHSPSEHTINGRRFALEAHL------EVFAVIGVLYKIGRPDYFLSKMKEYLEEV 185
           L+Q HWHSPSEHTINGRRFALE H+         AV+ VLYKIGR D F+  +++ LE +
Sbjct: 132 LQQLHWHSPSEHTINGRRFALELHMVHEGRNRRMAVVTVLYKIGRADTFIRSLEKELEGI 191

Query: 186 CDTKE-DKAIDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKVKTVSPEQVKLL 245
            + +E +K + + DP+++++ S  YYRY GSLT PPC+QNV W++VRKV+TV+ +QVKLL
Sbjct: 192 AEMEEAEKNVGMIDPTKIKIGSRKYYRYTGSLTTPPCTQNVTWSVVRKVRTVTRKQVKLL 251

Query: 246 RVAVHDDSDTNARPLQPLNDRKIQL 263
           RVAVHDD+++NARP+QP N R + L
Sbjct: 252 RVAVHDDANSNARPVQPTNKRIVHL 270

[Close]

BLAST of Carg23718-RA vs. TAIR 10
Match: AT1G08065.1 (alpha carbonic anhydrase 5 )

HSP 1 Score: 244.2 bits (622), Expect = 1.2e-64
Identity = 123/266 (46.24%), Postives = 176/266 (66.17%), Query Frame = 0


Query: 7   HALFCTFLCAFLLVSWPAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKAGLMQSP 66
           +  F  F+   ++ S P    EVED+ +F+Y ++G++GP +WG LK EW  C  G MQSP
Sbjct: 8   YVFFLIFISITIVSSSPDHG-EVEDETQFNYEKKGEKGPENWGRLKPEWAMCGKGNMQSP 67

Query: 67  IDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMNTHLSMDCDGAGY-MEVNGTRYFL 126
           IDL  +RV I  +   L+++Y PSNAT+KNRGHDIM   +  +   AG  + +NGT Y L
Sbjct: 68  IDLTDKRVLIDHNLGYLRSQYLPSNATIKNRGHDIM---MKFEGGNAGLGITINGTEYKL 127

Query: 127 KQFHWHSPSEHTINGRRFALEAHL------EVFAVIGVLYKIGRPDYFLSKMKEYLEEVC 186
           +Q HWHSPSEHT+NG+RF LE H+         AV+   YK+G+PDYFL  ++ YL+ + 
Sbjct: 128 QQIHWHSPSEHTLNGKRFVLEEHMVHQSKDGRNAVVAFFYKLGKPDYFLLTLERYLKRIT 187

Query: 187 DTKEDKA-IDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKVKTVSPEQVKLLR 246
           DT E +  +++  P      S  YYR+IGSLT PPCS+NV+WTI ++++TV+ +Q+ +LR
Sbjct: 188 DTHESQEFVEMVHPRTFGFESKHYYRFIGSLTTPPCSENVIWTISKEMRTVTLKQLIMLR 247

Query: 247 VAVHDDSDTNARPLQPLNDRKIQLRV 265
           V VHD S++NARPLQ  N+R + L +
Sbjct: 248 VTVHDQSNSNARPLQRKNERPVALYI 269

[Close]

BLAST of Carg23718-RA vs. TAIR 10
Match: AT2G28210.1 (alpha carbonic anhydrase 2 )

HSP 1 Score: 227.6 bits (579), Expect = 1.1e-59
Identity = 112/211 (53.08%), Postives = 142/211 (67.30%), Query Frame = 0


Query: 25  MSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKAGLMQSPIDLLHERVRIVPHFTNLK 84
           M + VED+ EF Y    + GP+ WG L+ EW  C  G MQSPIDL+++RVR+V H   L 
Sbjct: 1   MDEYVEDEHEFSYEWNQENGPAKWGKLRPEWKMCGKGEMQSPIDLMNKRVRLVTHLKKLT 60

Query: 85  TEYKPSNATLKNRGHDIMNTHLSMDCDGAGYMEVNGTRYFLKQFHWHSPSEHTINGRRFA 144
             YKP NATLKNRGHD+M   L    +G+G + VNGT Y L Q HWHSPSEHT+NGRRFA
Sbjct: 61  RHYKPCNATLKNRGHDMM---LKFGEEGSGSITVNGTEYKLLQLHWHSPSEHTMNGRRFA 120

Query: 145 LEAHL------EVFAVIGVLYKIGRPDYFLSKMKEYLEEVCDTKE-DKAIDLADPSQLEM 204
           LE H+         AV+ VLYKIGRPD FL  ++  L  + D  E +K +D+ DP  +++
Sbjct: 121 LELHMVHENINGSLAVVTVLYKIGRPDSFLGLLENKLSAITDQNEAEKYVDVIDPRDIKI 180

Query: 205 LSSLYYRYIGSLTVPPCSQNVLWTIVRKVKT 229
            S  +YRYIGSLT PPC+QNV+WT+V+KV T
Sbjct: 181 GSRKFYRYIGSLTTPPCTQNVIWTVVKKVNT 208

[Close]

BLAST of Carg23718-RA vs. TAIR 10
Match: AT4G20990.1 (alpha carbonic anhydrase 4 )

HSP 1 Score: 204.1 bits (518), Expect = 1.4e-52
Identity = 110/261 (42.15%), Postives = 161/261 (61.69%), Query Frame = 0


Query: 9   LFCTFLCAFLLVSWPAMS---QEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKAGLMQS 68
           +F   +C F+ +S+P +S    EV+D+  F Y ++ ++GP  WG +   W  C  G  QS
Sbjct: 8   IFFMAMC-FIYLSFPNISHAHSEVDDETPFTYEQKTEKGPEGWGKINPHWKVCNTGRYQS 67

Query: 69  PIDLLHERVRIVPHFTNLKTEYKPSNATLKNRGHDIMNTHLSMDCDGAGYMEVNGTRYFL 128
           PIDL +ERV ++ H      +YKP+ A + NRGHDIM   +S   D AG M +  T + L
Sbjct: 68  PIDLTNERVSLI-HDQAWTRQYKPAPAVITNRGHDIM---VSWKGD-AGKMTIRKTDFNL 127

Query: 129 KQFHWHSPSEHTINGRRFALEAHL------EVFAVIGVLYKIGRPDYFLSKMKEYLEEVC 188
            Q HWHSPSEHT+NG R+ LE H+         AVIGVLYK+G P+ FL+K+   ++ V 
Sbjct: 128 VQCHWHSPSEHTVNGTRYDLELHMVHTSARGRTAVIGVLYKLGEPNEFLTKLLNGIKAV- 187

Query: 189 DTKEDKAIDLADPSQLEMLSSLYYRYIGSLTVPPCSQNVLWTIVRKVKTVSPEQVKLLRV 248
              ++  + + DP ++   +  +YRYIGSLTVPPC++ V+WT+V++V T+S EQ+  LR 
Sbjct: 188 -GNKEINLGMIDPREIRFQTRKFYRYIGSLTVPPCTEGVIWTVVKRVNTISMEQITALRQ 247

Query: 249 AVHDDSDTNARPLQPLNDRKI 261
           AV D  +TN+RP+Q    R +
Sbjct: 248 AVDDGFETNSRPVQDSKGRSV 260

[Close]

BLAST of Carg23718-RA vs. TAIR 10
Match: AT5G56330.1 (alpha carbonic anhydrase 8 )

HSP 1 Score: 188.0 bits (476), Expect = 1.0e-47
Identity = 106/246 (43.09%), Postives = 137/246 (55.69%), Query Frame = 0


Query: 23  PAMSQEVEDQKEFDYNEEGKRGPSHWGDLKREWHSCKAGLMQSPIDLLHERVRIVPHFTN 82
           PA   EVED+ EF Y  +G +GP+ WG L  EW  C  G MQSPIDL  + V +   F  
Sbjct: 128 PAPGGEVEDETEFSYETKGNKGPAKWGTLDAEWKMCGIGKMQSPIDLRDKNVVVSNKFGL 187

Query: 83  LKTEYKPSNATLKNRGHDIMNTHLSMDCDGAGYMEVNGTRYFLKQFHWHSPSEHTINGRR 142
           L+++Y PSN T+KNRGHDIM         G G + + GTRY L+Q HWHSPSEHTING+R
Sbjct: 188 LRSQYLPSNTTIKNRGHDIM-LKFKGGNKGIG-VTIRGTRYQLQQLHWHSPSEHTINGKR 247

Query: 143 FALEAHL------EVFAVIGVLYKIGRPDYFLSKMKEYLEEVCDTKEDKAIDLADPSQLE 202
           FALE HL      + +AV+  LY +G  D FL  +++ L+++ DT   +           
Sbjct: 248 FALEEHLVHESKDKRYAVVAFLYNLGASDPFLFSLEKQLKKITDTHASE----------- 307

Query: 203 MLSSLYYRYIGSLTVPPCSQNVLWTIVRKVKTVSPEQVKLLRVAVHDDSDTNARPLQPLN 262
                                        ++TVS +QVKLLRVAVHD SD+NARPLQ +N
Sbjct: 308 ---------------------------EHIRTVSSKQVKLLRVAVHDASDSNARPLQAVN 333

The following BLAST results are available for this feature:

BLAST of Carg23718-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7020783.1	7.5e-154	100.00	Alpha carbonic anhydrase 7, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG7020784.1	1.2e-140	93.01	Alpha carbonic anhydrase 7, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6583573.1	1.6e-140	93.01	Alpha carbonic anhydrase 7, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG6583574.1	2.1e-140	92.65	Alpha carbonic anhydrase 7, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022964926.1	7.5e-138	91.18	alpha carbonic anhydrase 7-like [Cucurbita moschata]	[more]

BLAST of Carg23718-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L817	1.0e-73	53.96	Alpha carbonic anhydrase 7 OS=Arabidopsis thaliana OX=3702 GN=ACA7 PE=2 SV=1	[more]
Q9FM99	6.8e-65	50.61	Alpha carbonic anhydrase 8 OS=Arabidopsis thaliana OX=3702 GN=ACA8 PE=3 SV=3	[more]
F4HUC4	1.7e-63	46.24	Alpha carbonic anhydrase 5 OS=Arabidopsis thaliana OX=3702 GN=ACA5 PE=3 SV=1	[more]
Q84UV8	2.2e-63	49.23	Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3 OS...	[more]
F4IHR4	5.5e-59	49.37	Alpha carbonic anhydrase 2 OS=Arabidopsis thaliana OX=3702 GN=ACA2 PE=2 SV=2	[more]

BLAST of Carg23718-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HKB2	3.6e-138	91.18	Carbonic anhydrase OS=Cucurbita moschata OX=3662 GN=LOC111464883 PE=3 SV=1	[more]
A0A6J1HYT6	2.0e-136	90.07	Carbonic anhydrase OS=Cucurbita maxima OX=3661 GN=LOC111469328 PE=3 SV=1	[more]
A0A6J1I3K8	7.6e-136	90.07	Carbonic anhydrase OS=Cucurbita maxima OX=3661 GN=LOC111469329 PE=3 SV=1	[more]
A0A6J1HME8	5.9e-120	90.79	Carbonic anhydrase OS=Cucurbita moschata OX=3662 GN=LOC111464898 PE=3 SV=1	[more]
A0A6J1KFK2	1.6e-114	75.37	Carbonic anhydrase OS=Cucurbita maxima OX=3661 GN=LOC111493412 PE=3 SV=1	[more]

BLAST of Carg23718-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G08080.1	7.4e-75	53.96	alpha carbonic anhydrase 7	[more]
AT1G08065.1	1.2e-64	46.24	alpha carbonic anhydrase 5	[more]
AT2G28210.1	1.1e-59	53.08	alpha carbonic anhydrase 2	[more]
AT4G20990.1	1.4e-52	42.15	alpha carbonic anhydrase 4	[more]
AT5G56330.1	1.0e-47	43.09	alpha carbonic anhydrase 8	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001148	Alpha carbonic anhydrase domain	SMART	SM01057	Carb_anhydrase_2a	coord: 37..264 e-value: 1.5E-58 score: 210.5
IPR001148	Alpha carbonic anhydrase domain	PFAM	PF00194	Carb_anhydrase	coord: 36..261 e-value: 7.0E-37 score: 127.4
IPR001148	Alpha carbonic anhydrase domain	PROSITE	PS51144	ALPHA_CA_2	coord: 33..264 score: 44.177395
IPR036398	Alpha carbonic anhydrase domain superfamily	GENE3D	3.10.200.10	Alpha carbonic anhydrase	coord: 31..265 e-value: 8.7E-66 score: 223.8
IPR036398	Alpha carbonic anhydrase domain superfamily	SUPERFAMILY	51069	Carbonic anhydrase	coord: 31..261
None	No IPR available	PANTHER	PTHR18952:SF201	ALPHA CARBONIC ANHYDRASE 3	coord: 11..263
IPR023561	Carbonic anhydrase, alpha-class	PANTHER	PTHR18952	CARBONIC ANHYDRASE	coord: 11..263
IPR041891	Carbonic anhydrase, prokaryotic-like	CDD	cd03124	alpha_CA_prokaryotic_like	coord: 44..262 e-value: 9.40117E-85 score: 250.652

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg23718	Carg23718	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg23718-RA:8	Carg23718-RA:8	exon
Carg23718-RA:7	Carg23718-RA:7	exon
Carg23718-RA:6	Carg23718-RA:6	exon
Carg23718-RA:5	Carg23718-RA:5	exon
Carg23718-RA:4	Carg23718-RA:4	exon
Carg23718-RA:3	Carg23718-RA:3	exon
Carg23718-RA:2	Carg23718-RA:2	exon
Carg23718-RA:1	Carg23718-RA:1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg23718-RA:cds	Carg23718-RA:cds_8	CDS
Carg23718-RA:cds	Carg23718-RA:cds_7	CDS
Carg23718-RA:cds	Carg23718-RA:cds_6	CDS
Carg23718-RA:cds	Carg23718-RA:cds_5	CDS
Carg23718-RA:cds	Carg23718-RA:cds_4	CDS
Carg23718-RA:cds	Carg23718-RA:cds_3	CDS
Carg23718-RA:cds	Carg23718-RA:cds_2	CDS
Carg23718-RA:cds	Carg23718-RA:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg23718-RA	Carg23718-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006730	one-carbon metabolic process
cellular_component	GO:0009570	chloroplast stroma
molecular_function	GO:0004089	carbonate dehydratase activity
molecular_function	GO:0008270	zinc ion binding