Carg22070-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg22070-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Cold regulated protein 27, putative isoform 2
Location	Carg_Chr19: 6259133 .. 6261816 (+)
Sequence length	1113
RNA-Seq Expression	Carg22070-RA
Synteny	Carg22070-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCTCTCTCTCTCTCTCTCTCTCTTCGTATCTGCACGAGCCTGAATCCCGAGAGGGTTTCGGACCTAAATTTTCGCCCACGATTTCCATTTTTTCTTTGATTTGATTTTCTATATTTGGCTGTTCCGAACGATTTCGCTTTGTCTTTGATCTCCCGGAATGGTAGACGCTCATACGCCGTCGTCCATGTCGACGCCGGTTGCAGATCGAGAATCACCCATGTCGGTCTCTGCTAGCGACTTGACTCGTTTGAACTCCGAATCCTACTCTTCTTCCAATACCGTTGATGCGTCCGAGGGGGATTTGCCTTTGATGCCTTCTCATCATTGTTTTCAACTGGATGCGGTAAATTGTGACTCTATCTCTTTTGTTTTTGCTGTTTCTTGGAAGTATGATTTTGAGTTTTTCCTTGGTATGAACGAGTTTTTTGCGGACTGTGCTTTGTTTTTCTGCTTTGAACGTAGCGCCTGGAGTTCGTGTGACCTAGTTTTTGTGCTCTCGAAACTAATATCCTTGGTTACGAGTGATTCTTTTTCTCTTGGTGGTGTCTTTAGAAATTTTTAATGGTAGAGTGGTTTTGGGCACCTCCCATTTGAATGTTTAGTGTTAGGGCATATTCAAGTTAAATCGTGGATGAATTTCGAGTTAAGTCTTTGTGATTCAATTTTGTTTTTTTGTTTTTAAAAGAAAACATTTTACTCTTTGAATTCCCCTTGTTTTAGTTGTTATTTTCTCTTGTAGGACACTCATTTTATTTGTATCAAATGTTTTAGCTCTAAGTTTTAACTCATATTAAGGAACAATTTGAAAATTTCACGTGAAACATGAGGAATGGTTTTACTAATTGTTTTACTAATTGGAAATTTCAGGTGCAAACACATTGGACAGAAGAGAAACACAGATTATTTCTTGATTCTTTAGAAGCATCTTTTGTCCAAGATTTGCATCGATATAGGCATCTTCGTGCTTTGTCCTCGAAACAGAAAATGCGTCGGAAGCCGATCATGCCTAATATATCTTCTTCAGAGGTGAGGCAATACATCCACAGCAAGAGTGGGAGTGAGTTCCCTATATCATTCCTCTACGTCCTTAATGATTTCCTCTTGTTTTATTTCTTTTCTTAGTCGATGGTCCTTCAGGATGCCTCCTGGCAGAAGGCTAACGTTGGGAGAACTGATCCTTTATTGGATAAGACTGCCGATTCTCCCGCTATTTTAGAGGATGTATGGATAAATCATTTCCCTTCTGCAGGAAATCAGTGTACTCTGGAAACTACTGATGTTCTTGAAGAATGTGGAAGTTGTAGTGATAGGAGTCACATAAGAAACAAGACAAACTCCCGTCAATCTGCCAGAAGTTCTCAGCAGATTCCTCCATGCTGTCGTGATTCTCATACAATCGTTACAGGTACCCGAACACATTGTTCATGTTTTCCAAACCCTTTTCCAGAGTCTCACTTTTCGTTGATTCTACCGTCTAATATACTTAAAATTTGTAATCTATTCCCTTATTATGCATGTACACTATTGATGATGATCGGCCATATGCTTCATACTTTGTTGTTGTGATCTTCCAATCATATTTCGTAATGTTGAAGGTGTTTGCAATAATTATACTAGTAGTAGTATTGACTTAAGCACCATTTAGATGCACTATTTCTCTGATTTAAGTTTGTGTTGGTGTACCAATATGACCATTACTTTGTGATGTTACCATTAGTTGCCACATTGACTGCACCACTTGAAGTCCTGCAAACGTTTTTGCTTGAATTTTGTGTTCATCAAATGTGTTTCCTATGAGAGGGTTTCTGAAATACGACTTAGTGCATCCGAGTTGTAAAATTTGTACGTCAAGCTTAGGATATTATGTTATCTTCCTAGCAATGGAAAATCTATATGTTCTTAATCGGATAGTTTCAATTTGGTTTTCAGTGTTTTTGTAACTTTGTTCCCTTAGCTTGATTTATAGTGTTTACACCTGGGTCACAAGATTTAACGTCACAGTTCTTCATTCTATGTTACAATTAAGAACTCACGGGCAAACTGATTGCGTATCTTATCAATTTGAAGTTGTTTGATGTATATTTATGCTTGAAGAAGTAGAAGTCCTTTATGTAAAGTTGGATGAACATTGTTGTACGGGAAAAAAAAAGAGAATAAAACCGAGTAGAAATTACTAGGATGGACAGCATTCTGATTATTGATGAACCTCTAGATGAAGCCAGGCTAATGTAGACTGATTCTAGGACAGTAGCTGTTTAGGGATGAATCAAAGCAGAAATTCTTGGGTTCATAACATTTCAGTCAAATTAGAGCATCCATGTTAAGGAATTTGCCTTCTTTTATGAATGTTTAATGCTTGATGTGATTTTTCTGTTTTCATACTAACTTAGTGTCTATTATCTCTTCATTGCAGAAGTTTCAGATCAAAACTTTGTGGATACTCATCCAGGAGAGTTGTCTGATGGCACGCCCATTACAAAACGATTAAAGAAAGCTTCCTCTGATTCTTCAGGCAACAACCGGGTAAAACTCGGGATTGCCATTGATTTCTCTTTGGAGAACCAATAATTGCATTTTACAAATGATCCAAGACCGAATATGCTGCTATTTCATCTCTTAGGTTGCACCATATAAAAGATTATCATCAGAAAGATAAAAGCCAGATGCAAAAACTTTGTGCCTCC

mRNA sequence

TCTCTCTCTCTCTCTCTCTCTCTTCGTATCTGCACGAGCCTGAATCCCGAGAGGGTTTCGGACCTAAATTTTCGCCCACGATTTCCATTTTTTCTTTGATTTGATTTTCTATATTTGGCTGTTCCGAACGATTTCGCTTTGTCTTTGATCTCCCGGAATGGTAGACGCTCATACGCCGTCGTCCATGTCGACGCCGGTTGCAGATCGAGAATCACCCATGTCGGTCTCTGCTAGCGACTTGACTCGTTTGAACTCCGAATCCTACTCTTCTTCCAATACCGTTGATGCGTCCGAGGGGGATTTGCCTTTGATGCCTTCTCATCATTGTTTTCAACTGGATGCGGTGCAAACACATTGGACAGAAGAGAAACACAGATTATTTCTTGATTCTTTAGAAGCATCTTTTGTCCAAGATTTGCATCGATATAGGCATCTTCGTGCTTTGTCCTCGAAACAGAAAATGCGTCGGAAGCCGATCATGCCTAATATATCTTCTTCAGAGGTGAGGCAATACATCCACAGCAAGAGTGGGAGTGAGTTCCCTATATCATTCCTCTACTCGATGGTCCTTCAGGATGCCTCCTGGCAGAAGGCTAACGTTGGGAGAACTGATCCTTTATTGGATAAGACTGCCGATTCTCCCGCTATTTTAGAGGATGTATGGATAAATCATTTCCCTTCTGCAGGAAATCAGTGTACTCTGGAAACTACTGATGTTCTTGAAGAATGTGGAAGTTGTAGTGATAGGAGTCACATAAGAAACAAGACAAACTCCCGTCAATCTGCCAGAAGTTCTCAGCAGATTCCTCCATGCTGTCGTGATTCTCATACAATCGTTACAGAAGTTTCAGATCAAAACTTTGTGGATACTCATCCAGGAGAGTTGTCTGATGGCACGCCCATTACAAAACGATTAAAGAAAGCTTCCTCTGATTCTTCAGGCAACAACCGGGTAAAACTCGGGATTGCCATTGATTTCTCTTTGGAGAACCAATAATTGCATTTTACAAATGATCCAAGACCGAATATGCTGCTATTTCATCTCTTAGGTTGCACCATATAAAAGATTATCATCAGAAAGATAAAAGCCAGATGCAAAAACTTTGTGCCTCC

Coding sequence (CDS)

ATGGTAGACGCTCATACGCCGTCGTCCATGTCGACGCCGGTTGCAGATCGAGAATCACCCATGTCGGTCTCTGCTAGCGACTTGACTCGTTTGAACTCCGAATCCTACTCTTCTTCCAATACCGTTGATGCGTCCGAGGGGGATTTGCCTTTGATGCCTTCTCATCATTGTTTTCAACTGGATGCGGTGCAAACACATTGGACAGAAGAGAAACACAGATTATTTCTTGATTCTTTAGAAGCATCTTTTGTCCAAGATTTGCATCGATATAGGCATCTTCGTGCTTTGTCCTCGAAACAGAAAATGCGTCGGAAGCCGATCATGCCTAATATATCTTCTTCAGAGGTGAGGCAATACATCCACAGCAAGAGTGGGAGTGAGTTCCCTATATCATTCCTCTACTCGATGGTCCTTCAGGATGCCTCCTGGCAGAAGGCTAACGTTGGGAGAACTGATCCTTTATTGGATAAGACTGCCGATTCTCCCGCTATTTTAGAGGATGTATGGATAAATCATTTCCCTTCTGCAGGAAATCAGTGTACTCTGGAAACTACTGATGTTCTTGAAGAATGTGGAAGTTGTAGTGATAGGAGTCACATAAGAAACAAGACAAACTCCCGTCAATCTGCCAGAAGTTCTCAGCAGATTCCTCCATGCTGTCGTGATTCTCATACAATCGTTACAGAAGTTTCAGATCAAAACTTTGTGGATACTCATCCAGGAGAGTTGTCTGATGGCACGCCCATTACAAAACGATTAAAGAAAGCTTCCTCTGATTCTTCAGGCAACAACCGGGTAAAACTCGGGATTGCCATTGATTTCTCTTTGGAGAACCAATAA

Protein sequence

MVDAHTPSSMSTPVADRESPMSVSASDLTRLNSESYSSSNTVDASEGDLPLMPSHHCFQLDAVQTHWTEEKHRLFLDSLEASFVQDLHRYRHLRALSSKQKMRRKPIMPNISSSEVRQYIHSKSGSEFPISFLYSMVLQDASWQKANVGRTDPLLDKTADSPAILEDVWINHFPSAGNQCTLETTDVLEECGSCSDRSHIRNKTNSRQSARSSQQIPPCCRDSHTIVTEVSDQNFVDTHPGELSDGTPITKRLKKASSDSSGNNRVKLGIAIDFSLENQ

Homology

[Close]

BLAST of Carg22070-RA vs. NCBI nr
Match: KAG7011651.1 (hypothetical protein SDJN02_26557 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 554.3 bits (1427), Expect = 6.0e-154
Identity = 279/279 (100.00%), Postives = 279/279 (100.00%), Query Frame = 0


Query: 1   MVDAHTPSSMSTPVADRESPMSVSASDLTRLNSESYSSSNTVDASEGDLPLMPSHHCFQL 60
           MVDAHTPSSMSTPVADRESPMSVSASDLTRLNSESYSSSNTVDASEGDLPLMPSHHCFQL
Sbjct: 1   MVDAHTPSSMSTPVADRESPMSVSASDLTRLNSESYSSSNTVDASEGDLPLMPSHHCFQL 60

Query: 61  DAVQTHWTEEKHRLFLDSLEASFVQDLHRYRHLRALSSKQKMRRKPIMPNISSSEVRQYI 120
           DAVQTHWTEEKHRLFLDSLEASFVQDLHRYRHLRALSSKQKMRRKPIMPNISSSEVRQYI
Sbjct: 61  DAVQTHWTEEKHRLFLDSLEASFVQDLHRYRHLRALSSKQKMRRKPIMPNISSSEVRQYI 120

Query: 121 HSKSGSEFPISFLYSMVLQDASWQKANVGRTDPLLDKTADSPAILEDVWINHFPSAGNQC 180
           HSKSGSEFPISFLYSMVLQDASWQKANVGRTDPLLDKTADSPAILEDVWINHFPSAGNQC
Sbjct: 121 HSKSGSEFPISFLYSMVLQDASWQKANVGRTDPLLDKTADSPAILEDVWINHFPSAGNQC 180

Query: 181 TLETTDVLEECGSCSDRSHIRNKTNSRQSARSSQQIPPCCRDSHTIVTEVSDQNFVDTHP 240
           TLETTDVLEECGSCSDRSHIRNKTNSRQSARSSQQIPPCCRDSHTIVTEVSDQNFVDTHP
Sbjct: 181 TLETTDVLEECGSCSDRSHIRNKTNSRQSARSSQQIPPCCRDSHTIVTEVSDQNFVDTHP 240

Query: 241 GELSDGTPITKRLKKASSDSSGNNRVKLGIAIDFSLENQ 280
           GELSDGTPITKRLKKASSDSSGNNRVKLGIAIDFSLENQ
Sbjct: 241 GELSDGTPITKRLKKASSDSSGNNRVKLGIAIDFSLENQ 279

[Close]

BLAST of Carg22070-RA vs. NCBI nr
Match: XP_022952080.1 (uncharacterized protein LOC111454842 isoform X1 [Cucurbita moschata])

HSP 1 Score: 502.3 bits (1292), Expect = 2.7e-138
Identity = 259/279 (92.83%), Postives = 259/279 (92.83%), Query Frame = 0


Query: 1   MVDAHTPSSMSTPVADRESPMSVSASDLTRLNSESYSSSNTVDASEGDLPLMPSHHCFQL 60
           MVDAHTPSSMS PVADRESPMSVSASDLTRLNSESYSSSNTVDASEGDLPLMPSHHCFQL
Sbjct: 1   MVDAHTPSSMSPPVADRESPMSVSASDLTRLNSESYSSSNTVDASEGDLPLMPSHHCFQL 60

Query: 61  DAVQTHWTEEKHRLFLDSLEASFVQDLHRYRHLRALSSKQKMRRKPIMPNISSSEVRQYI 120
           DAVQTHWTEEKHRLFLDSLEASFVQDLHRYRHLRALSSKQKMRRKPIMPNISSSE     
Sbjct: 61  DAVQTHWTEEKHRLFLDSLEASFVQDLHRYRHLRALSSKQKMRRKPIMPNISSSE----- 120

Query: 121 HSKSGSEFPISFLYSMVLQDASWQKANVGRTDPLLDKTADSPAILEDVWINHFPSAGNQC 180
                         SMVLQDASWQKANVGRTDPLLDKTADSPAILEDVWINHFPSAGNQC
Sbjct: 121 --------------SMVLQDASWQKANVGRTDPLLDKTADSPAILEDVWINHFPSAGNQC 180

Query: 181 TLETTDVLEECGSCSDRSHIRNKTNSRQSARSSQQIPPCCRDSHTIVTEVSDQNFVDTHP 240
           TLETTDVLEECGSCSDRSHIRNKTNSRQSARSSQQIPPCCRDSHTIVTEVSDQNFVDTHP
Sbjct: 181 TLETTDVLEECGSCSDRSHIRNKTNSRQSARSSQQIPPCCRDSHTIVTEVSDQNFVDTHP 240

Query: 241 GELSDGTPITKRLKKASSDSSGNNRVKLGIAIDFSLENQ 280
           GELSDGTPITKRLKKASSDSSGNNRVKLGIAIDFSLENQ
Sbjct: 241 GELSDGTPITKRLKKASSDSSGNNRVKLGIAIDFSLENQ 260

[Close]

BLAST of Carg22070-RA vs. NCBI nr
Match: XP_022952081.1 (uncharacterized protein LOC111454842 isoform X2 [Cucurbita moschata])

HSP 1 Score: 477.6 bits (1228), Expect = 7.2e-131
Identity = 246/266 (92.48%), Postives = 246/266 (92.48%), Query Frame = 0


Query: 1   MVDAHTPSSMSTPVADRESPMSVSASDLTRLNSESYSSSNTVDASEGDLPLMPSHHCFQL 60
           MVDAHTPSSMS PVADRESPMSVSASDLTRLNSESYSSSNTVDASEGDLPLMPSHHCFQL
Sbjct: 1   MVDAHTPSSMSPPVADRESPMSVSASDLTRLNSESYSSSNTVDASEGDLPLMPSHHCFQL 60

Query: 61  DAVQTHWTEEKHRLFLDSLEASFVQDLHRYRHLRALSSKQKMRRKPIMPNISSSEVRQYI 120
           DAVQTHWTEEKHRLFLDSLEASFVQDLHRYRHLRALSSKQKMRRKPIMPNISSSE     
Sbjct: 61  DAVQTHWTEEKHRLFLDSLEASFVQDLHRYRHLRALSSKQKMRRKPIMPNISSSE----- 120

Query: 121 HSKSGSEFPISFLYSMVLQDASWQKANVGRTDPLLDKTADSPAILEDVWINHFPSAGNQC 180
                         SMVLQDASWQKANVGRTDPLLDKTADSPAILEDVWINHFPSAGNQC
Sbjct: 121 --------------SMVLQDASWQKANVGRTDPLLDKTADSPAILEDVWINHFPSAGNQC 180

Query: 181 TLETTDVLEECGSCSDRSHIRNKTNSRQSARSSQQIPPCCRDSHTIVTEVSDQNFVDTHP 240
           TLETTDVLEECGSCSDRSHIRNKTNSRQSARSSQQIPPCCRDSHTIVTEVSDQNFVDTHP
Sbjct: 181 TLETTDVLEECGSCSDRSHIRNKTNSRQSARSSQQIPPCCRDSHTIVTEVSDQNFVDTHP 240

Query: 241 GELSDGTPITKRLKKASSDSSGNNRV 267
           GELSDGTPITKRLKKASSDSSGNNRV
Sbjct: 241 GELSDGTPITKRLKKASSDSSGNNRV 247

[Close]

BLAST of Carg22070-RA vs. NCBI nr
Match: XP_023553838.1 (uncharacterized protein LOC111811294 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 469.5 bits (1207), Expect = 2.0e-128
Identity = 243/279 (87.10%), Postives = 248/279 (88.89%), Query Frame = 0


Query: 1   MVDAHTPSSMSTPVADRESPMSVSASDLTRLNSESYSSSNTVDASEGDLPLMPSHHCFQL 60
           MVDAHTPSSMS PVADRESPMSVSASDLTRLNSESYSSSNTVDASEGDLPLMPSHHCFQL
Sbjct: 1   MVDAHTPSSMSPPVADRESPMSVSASDLTRLNSESYSSSNTVDASEGDLPLMPSHHCFQL 60

Query: 61  DAVQTHWTEEKHRLFLDSLEASFVQDLHRYRHLRALSSKQKMRRKPIMPNISSSEVRQYI 120
           DAVQTHWT+EKHRLFLDSLEASFVQDLHRYRHLRA SSKQK+RRK IMPNISSSE     
Sbjct: 61  DAVQTHWTDEKHRLFLDSLEASFVQDLHRYRHLRAFSSKQKVRRKLIMPNISSSE----- 120

Query: 121 HSKSGSEFPISFLYSMVLQDASWQKANVGRTDPLLDKTADSPAILEDVWINHFPSAGNQC 180
                         S+VLQDASWQKAN+GRTDPLL KTADSPAILEDVWINHFPSAG  C
Sbjct: 121 --------------SLVLQDASWQKANIGRTDPLLHKTADSPAILEDVWINHFPSAGKPC 180

Query: 181 TLETTDVLEECGSCSDRSHIRNKTNSRQSARSSQQIPPCCRDSHTIVTEVSDQNFVDTHP 240
           TLETTDVLEECGSCSDR HIRNKTNS QSARSSQQIP CCRDSHTI TEVSDQNFVDTHP
Sbjct: 181 TLETTDVLEECGSCSDRRHIRNKTNSCQSARSSQQIPSCCRDSHTIFTEVSDQNFVDTHP 240

Query: 241 GELSDGTPITKRLKKASSDSSGNNRVKLGIAIDFSLENQ 280
           GELSDGTPITKRLKKASSDSSGN+RVKLGIA D SLENQ
Sbjct: 241 GELSDGTPITKRLKKASSDSSGNDRVKLGIAFDLSLENQ 260

[Close]

BLAST of Carg22070-RA vs. NCBI nr
Match: XP_022972372.1 (uncharacterized protein LOC111470946 isoform X1 [Cucurbita maxima])

HSP 1 Score: 469.2 bits (1206), Expect = 2.5e-128
Identity = 244/279 (87.46%), Postives = 248/279 (88.89%), Query Frame = 0


Query: 1   MVDAHTPSSMSTPVADRESPMSVSASDLTRLNSESYSSSNTVDASEGDLPLMPSHHCFQL 60
           MVDAHTPSSMS PVADRES MSVSASDLTRLNSESYSSSNTVDASEGDLPLMPSHHCFQL
Sbjct: 1   MVDAHTPSSMSPPVADRESSMSVSASDLTRLNSESYSSSNTVDASEGDLPLMPSHHCFQL 60

Query: 61  DAVQTHWTEEKHRLFLDSLEASFVQDLHRYRHLRALSSKQKMRRKPIMPNISSSEVRQYI 120
           DAVQTHWT+EKHRLFLD+LEASFVQDLHRYRHLRA SSKQKMRRKPIMPNISSSE     
Sbjct: 61  DAVQTHWTDEKHRLFLDALEASFVQDLHRYRHLRAFSSKQKMRRKPIMPNISSSE----- 120

Query: 121 HSKSGSEFPISFLYSMVLQDASWQKANVGRTDPLLDKTADSPAILEDVWINHFPSAGNQC 180
                         SMVLQDASWQKAN+GRTDPLL KTADSPAILEDVWINHFPSAGNQ 
Sbjct: 121 --------------SMVLQDASWQKANIGRTDPLLHKTADSPAILEDVWINHFPSAGNQR 180

Query: 181 TLETTDVLEECGSCSDRSHIRNKTNSRQSARSSQQIPPCCRDSHTIVTEVSDQNFVDTHP 240
           T ETTDVLEECGSCSDRSHIRNKTNS QSARSSQQIPPCCRDSHTIV EVSDQNFVDTHP
Sbjct: 181 TPETTDVLEECGSCSDRSHIRNKTNSCQSARSSQQIPPCCRDSHTIVAEVSDQNFVDTHP 240

Query: 241 GELSDGTPITKRLKKASSDSSGNNRVKLGIAIDFSLENQ 280
           GELS GTPITKRLKKASSDS GN+RVKLGIA D SLENQ
Sbjct: 241 GELSGGTPITKRLKKASSDSLGNDRVKLGIAFDLSLENQ 260

[Close]

BLAST of Carg22070-RA vs. ExPASy Swiss-Prot
Match: Q8L8T7 (Cold-regulated protein 27 OS=Arabidopsis thaliana OX=3702 GN=COR27 PE=1 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 8.0e-08
Identity = 67/214 (31.31%), Postives = 94/214 (43.93%), Query Frame = 0


Query: 30  RLNSESYSSSNTVDASEGDLPLMPSHHCFQLDAVQTHWTEEKHRLFLDSLEASFVQDLHR 89
           R + +S  SS+   + EG+     S +    + ++T WT EKH L+L S+EASFV  L  
Sbjct: 13  RFSDDSDDSSDDASSVEGE--TTSSMYSAGKEYMETEWTNEKHSLYLKSMEASFVDQL-- 72

Query: 90  YRHLRALSSKQKMRRKPIMPNISSSEVRQYIHSKSGSEFPISFLYSMVLQDASWQKANVG 149
           Y  L AL   +         N+S S       ++ GS    S     VL D  WQK NV 
Sbjct: 73  YNSLGALGKNE---------NVSES-------TRFGSGRKPSQEQFKVLHDGFWQKINVK 132

Query: 150 RTDPLLD--KTADSPAILEDVWINHF-PSAGNQCTLETTDVLEECGSCSDRSHIRNKTNS 209
           + +  ++     +S   L   WI H+ P    Q  +  TD  E        S+ +    S
Sbjct: 133 QPEHRINGRHGGNSHEFLRSPWIKHYKPLVKTQ--IPVTD--EPENQVVSSSNGKKGICS 192

Query: 210 RQSARSSQQIPPCCRDSHTI---VTEVSDQNFVD 238
             SA S +Q+    RD   I     EVSDQNFV+
Sbjct: 193 SGSASSLKQLSSHSRDHDQISVGEAEVSDQNFVN 202

[Close]

BLAST of Carg22070-RA vs. ExPASy TrEMBL
Match: A0A6J1GKR5 (uncharacterized protein LOC111454842 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111454842 PE=4 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 1.3e-138
Identity = 259/279 (92.83%), Postives = 259/279 (92.83%), Query Frame = 0


Query: 1   MVDAHTPSSMSTPVADRESPMSVSASDLTRLNSESYSSSNTVDASEGDLPLMPSHHCFQL 60
           MVDAHTPSSMS PVADRESPMSVSASDLTRLNSESYSSSNTVDASEGDLPLMPSHHCFQL
Sbjct: 1   MVDAHTPSSMSPPVADRESPMSVSASDLTRLNSESYSSSNTVDASEGDLPLMPSHHCFQL 60

Query: 61  DAVQTHWTEEKHRLFLDSLEASFVQDLHRYRHLRALSSKQKMRRKPIMPNISSSEVRQYI 120
           DAVQTHWTEEKHRLFLDSLEASFVQDLHRYRHLRALSSKQKMRRKPIMPNISSSE     
Sbjct: 61  DAVQTHWTEEKHRLFLDSLEASFVQDLHRYRHLRALSSKQKMRRKPIMPNISSSE----- 120

Query: 121 HSKSGSEFPISFLYSMVLQDASWQKANVGRTDPLLDKTADSPAILEDVWINHFPSAGNQC 180
                         SMVLQDASWQKANVGRTDPLLDKTADSPAILEDVWINHFPSAGNQC
Sbjct: 121 --------------SMVLQDASWQKANVGRTDPLLDKTADSPAILEDVWINHFPSAGNQC 180

Query: 181 TLETTDVLEECGSCSDRSHIRNKTNSRQSARSSQQIPPCCRDSHTIVTEVSDQNFVDTHP 240
           TLETTDVLEECGSCSDRSHIRNKTNSRQSARSSQQIPPCCRDSHTIVTEVSDQNFVDTHP
Sbjct: 181 TLETTDVLEECGSCSDRSHIRNKTNSRQSARSSQQIPPCCRDSHTIVTEVSDQNFVDTHP 240

Query: 241 GELSDGTPITKRLKKASSDSSGNNRVKLGIAIDFSLENQ 280
           GELSDGTPITKRLKKASSDSSGNNRVKLGIAIDFSLENQ
Sbjct: 241 GELSDGTPITKRLKKASSDSSGNNRVKLGIAIDFSLENQ 260

[Close]

BLAST of Carg22070-RA vs. ExPASy TrEMBL
Match: A0A6J1GJA3 (uncharacterized protein LOC111454842 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111454842 PE=4 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 3.5e-131
Identity = 246/266 (92.48%), Postives = 246/266 (92.48%), Query Frame = 0


Query: 1   MVDAHTPSSMSTPVADRESPMSVSASDLTRLNSESYSSSNTVDASEGDLPLMPSHHCFQL 60
           MVDAHTPSSMS PVADRESPMSVSASDLTRLNSESYSSSNTVDASEGDLPLMPSHHCFQL
Sbjct: 1   MVDAHTPSSMSPPVADRESPMSVSASDLTRLNSESYSSSNTVDASEGDLPLMPSHHCFQL 60

Query: 61  DAVQTHWTEEKHRLFLDSLEASFVQDLHRYRHLRALSSKQKMRRKPIMPNISSSEVRQYI 120
           DAVQTHWTEEKHRLFLDSLEASFVQDLHRYRHLRALSSKQKMRRKPIMPNISSSE     
Sbjct: 61  DAVQTHWTEEKHRLFLDSLEASFVQDLHRYRHLRALSSKQKMRRKPIMPNISSSE----- 120

Query: 121 HSKSGSEFPISFLYSMVLQDASWQKANVGRTDPLLDKTADSPAILEDVWINHFPSAGNQC 180
                         SMVLQDASWQKANVGRTDPLLDKTADSPAILEDVWINHFPSAGNQC
Sbjct: 121 --------------SMVLQDASWQKANVGRTDPLLDKTADSPAILEDVWINHFPSAGNQC 180

Query: 181 TLETTDVLEECGSCSDRSHIRNKTNSRQSARSSQQIPPCCRDSHTIVTEVSDQNFVDTHP 240
           TLETTDVLEECGSCSDRSHIRNKTNSRQSARSSQQIPPCCRDSHTIVTEVSDQNFVDTHP
Sbjct: 181 TLETTDVLEECGSCSDRSHIRNKTNSRQSARSSQQIPPCCRDSHTIVTEVSDQNFVDTHP 240

Query: 241 GELSDGTPITKRLKKASSDSSGNNRV 267
           GELSDGTPITKRLKKASSDSSGNNRV
Sbjct: 241 GELSDGTPITKRLKKASSDSSGNNRV 247

[Close]

BLAST of Carg22070-RA vs. ExPASy TrEMBL
Match: A0A6J1I4M4 (uncharacterized protein LOC111470946 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111470946 PE=4 SV=1)

HSP 1 Score: 469.2 bits (1206), Expect = 1.2e-128
Identity = 244/279 (87.46%), Postives = 248/279 (88.89%), Query Frame = 0


Query: 1   MVDAHTPSSMSTPVADRESPMSVSASDLTRLNSESYSSSNTVDASEGDLPLMPSHHCFQL 60
           MVDAHTPSSMS PVADRES MSVSASDLTRLNSESYSSSNTVDASEGDLPLMPSHHCFQL
Sbjct: 1   MVDAHTPSSMSPPVADRESSMSVSASDLTRLNSESYSSSNTVDASEGDLPLMPSHHCFQL 60

Query: 61  DAVQTHWTEEKHRLFLDSLEASFVQDLHRYRHLRALSSKQKMRRKPIMPNISSSEVRQYI 120
           DAVQTHWT+EKHRLFLD+LEASFVQDLHRYRHLRA SSKQKMRRKPIMPNISSSE     
Sbjct: 61  DAVQTHWTDEKHRLFLDALEASFVQDLHRYRHLRAFSSKQKMRRKPIMPNISSSE----- 120

Query: 121 HSKSGSEFPISFLYSMVLQDASWQKANVGRTDPLLDKTADSPAILEDVWINHFPSAGNQC 180
                         SMVLQDASWQKAN+GRTDPLL KTADSPAILEDVWINHFPSAGNQ 
Sbjct: 121 --------------SMVLQDASWQKANIGRTDPLLHKTADSPAILEDVWINHFPSAGNQR 180

Query: 181 TLETTDVLEECGSCSDRSHIRNKTNSRQSARSSQQIPPCCRDSHTIVTEVSDQNFVDTHP 240
           T ETTDVLEECGSCSDRSHIRNKTNS QSARSSQQIPPCCRDSHTIV EVSDQNFVDTHP
Sbjct: 181 TPETTDVLEECGSCSDRSHIRNKTNSCQSARSSQQIPPCCRDSHTIVAEVSDQNFVDTHP 240

Query: 241 GELSDGTPITKRLKKASSDSSGNNRVKLGIAIDFSLENQ 280
           GELS GTPITKRLKKASSDS GN+RVKLGIA D SLENQ
Sbjct: 241 GELSGGTPITKRLKKASSDSLGNDRVKLGIAFDLSLENQ 260

[Close]

BLAST of Carg22070-RA vs. ExPASy TrEMBL
Match: A0A6J1I9R8 (uncharacterized protein LOC111470946 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111470946 PE=4 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 2.3e-122
Identity = 233/266 (87.59%), Postives = 237/266 (89.10%), Query Frame = 0


Query: 1   MVDAHTPSSMSTPVADRESPMSVSASDLTRLNSESYSSSNTVDASEGDLPLMPSHHCFQL 60
           MVDAHTPSSMS PVADRES MSVSASDLTRLNSESYSSSNTVDASEGDLPLMPSHHCFQL
Sbjct: 1   MVDAHTPSSMSPPVADRESSMSVSASDLTRLNSESYSSSNTVDASEGDLPLMPSHHCFQL 60

Query: 61  DAVQTHWTEEKHRLFLDSLEASFVQDLHRYRHLRALSSKQKMRRKPIMPNISSSEVRQYI 120
           DAVQTHWT+EKHRLFLD+LEASFVQDLHRYRHLRA SSKQKMRRKPIMPNISSSE     
Sbjct: 61  DAVQTHWTDEKHRLFLDALEASFVQDLHRYRHLRAFSSKQKMRRKPIMPNISSSE----- 120

Query: 121 HSKSGSEFPISFLYSMVLQDASWQKANVGRTDPLLDKTADSPAILEDVWINHFPSAGNQC 180
                         SMVLQDASWQKAN+GRTDPLL KTADSPAILEDVWINHFPSAGNQ 
Sbjct: 121 --------------SMVLQDASWQKANIGRTDPLLHKTADSPAILEDVWINHFPSAGNQR 180

Query: 181 TLETTDVLEECGSCSDRSHIRNKTNSRQSARSSQQIPPCCRDSHTIVTEVSDQNFVDTHP 240
           T ETTDVLEECGSCSDRSHIRNKTNS QSARSSQQIPPCCRDSHTIV EVSDQNFVDTHP
Sbjct: 181 TPETTDVLEECGSCSDRSHIRNKTNSCQSARSSQQIPPCCRDSHTIVAEVSDQNFVDTHP 240

Query: 241 GELSDGTPITKRLKKASSDSSGNNRV 267
           GELS GTPITKRLKKASSDS GN+RV
Sbjct: 241 GELSGGTPITKRLKKASSDSLGNDRV 247

[Close]

BLAST of Carg22070-RA vs. ExPASy TrEMBL
Match: A0A6J1GJF7 (uncharacterized protein LOC111454842 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111454842 PE=4 SV=1)

HSP 1 Score: 419.5 bits (1077), Expect = 1.1e-113
Identity = 226/279 (81.00%), Postives = 226/279 (81.00%), Query Frame = 0


Query: 1   MVDAHTPSSMSTPVADRESPMSVSASDLTRLNSESYSSSNTVDASEGDLPLMPSHHCFQL 60
           MVDAHTPSSMS PVADRESPMSVSASDLTRLNSESYSSSNTVDASEGDLPLMPSHHCFQL
Sbjct: 1   MVDAHTPSSMSPPVADRESPMSVSASDLTRLNSESYSSSNTVDASEGDLPLMPSHHCFQL 60

Query: 61  DAVQTHWTEEKHRLFLDSLEASFVQDLHRYRHLRALSSKQKMRRKPIMPNISSSEVRQYI 120
           DAVQTHWTEEKHRLFLDSLEASFVQDLHRYRHLRALSSKQKMRRKPIMPNISSSEVRQYI
Sbjct: 61  DAVQTHWTEEKHRLFLDSLEASFVQDLHRYRHLRALSSKQKMRRKPIMPNISSSEVRQYI 120

Query: 121 HSKSGSEFPISFLYSMVLQDASWQKANVGRTDPLLDKTADSPAILEDVWINHFPSAGNQC 180
           HSKSG                                                    NQC
Sbjct: 121 HSKSGR---------------------------------------------------NQC 180

Query: 181 TLETTDVLEECGSCSDRSHIRNKTNSRQSARSSQQIPPCCRDSHTIVTEVSDQNFVDTHP 240
           TLETTDVLEECGSCSDRSHIRNKTNSRQSARSSQQIPPCCRDSHTIVTEVSDQNFVDTHP
Sbjct: 181 TLETTDVLEECGSCSDRSHIRNKTNSRQSARSSQQIPPCCRDSHTIVTEVSDQNFVDTHP 228

Query: 241 GELSDGTPITKRLKKASSDSSGNNRVKLGIAIDFSLENQ 280
           GELSDGTPITKRLKKASSDSSGNNRVKLGIAIDFSLENQ
Sbjct: 241 GELSDGTPITKRLKKASSDSSGNNRVKLGIAIDFSLENQ 228

[Close]

BLAST of Carg22070-RA vs. TAIR 10
Match: AT5G42900.3 (cold regulated gene 27 )

HSP 1 Score: 59.7 bits (143), Expect = 4.3e-09
Identity = 67/213 (31.46%), Postives = 94/213 (44.13%), Query Frame = 0


Query: 30  RLNSESYSSSNTVDASEGDLPLMPSHHCFQLDAVQTHWTEEKHRLFLDSLEASFVQDLHR 89
           R + +S  SS+   + EG+     S +    + ++T WT EKH L+L S+EASFV  L  
Sbjct: 13  RFSDDSDDSSDDASSVEGE--TTSSMYSAGKEYMETEWTNEKHSLYLKSMEASFVDQL-- 72

Query: 90  YRHLRALSSKQKMRRKPIMPNISSSEVRQYIHSKSGSEFPISFLYSMVLQDASWQKANVG 149
           Y  L AL   +         N+S S       ++ GS    S     VL D  WQK NV 
Sbjct: 73  YNSLGALGKNE---------NVSES-------TRFGSGRKPSQEQFKVLHDGFWQKINVK 132

Query: 150 RTDPLLD--KTADSPAILEDVWINHF-PSAGNQCTLETTDVLEECGSCSDRSHIRNKTNS 209
           + +  ++     +S   L   WI H+ P    Q  +  TD  E        S+ +    S
Sbjct: 133 QPEHRINGRHGGNSHEFLRSPWIKHYKPLVKTQ--IPVTD--EPENQVVSSSNGKKGICS 192

Query: 210 RQSARSSQQIPPCCRDSHTIVT--EVSDQNFVD 238
             SA S +Q+    RD   I    EVSDQNFV+
Sbjct: 193 SGSASSLKQLSSHSRDHDQISVGEEVSDQNFVN 201

[Close]

BLAST of Carg22070-RA vs. TAIR 10
Match: AT5G42900.1 (cold regulated gene 27 )

HSP 1 Score: 59.3 bits (142), Expect = 5.7e-09
Identity = 67/214 (31.31%), Postives = 94/214 (43.93%), Query Frame = 0


Query: 30  RLNSESYSSSNTVDASEGDLPLMPSHHCFQLDAVQTHWTEEKHRLFLDSLEASFVQDLHR 89
           R + +S  SS+   + EG+     S +    + ++T WT EKH L+L S+EASFV  L  
Sbjct: 13  RFSDDSDDSSDDASSVEGE--TTSSMYSAGKEYMETEWTNEKHSLYLKSMEASFVDQL-- 72

Query: 90  YRHLRALSSKQKMRRKPIMPNISSSEVRQYIHSKSGSEFPISFLYSMVLQDASWQKANVG 149
           Y  L AL   +         N+S S       ++ GS    S     VL D  WQK NV 
Sbjct: 73  YNSLGALGKNE---------NVSES-------TRFGSGRKPSQEQFKVLHDGFWQKINVK 132

Query: 150 RTDPLLD--KTADSPAILEDVWINHF-PSAGNQCTLETTDVLEECGSCSDRSHIRNKTNS 209
           + +  ++     +S   L   WI H+ P    Q  +  TD  E        S+ +    S
Sbjct: 133 QPEHRINGRHGGNSHEFLRSPWIKHYKPLVKTQ--IPVTD--EPENQVVSSSNGKKGICS 192

Query: 210 RQSARSSQQIPPCCRDSHTI---VTEVSDQNFVD 238
             SA S +Q+    RD   I     EVSDQNFV+
Sbjct: 193 SGSASSLKQLSSHSRDHDQISVGEAEVSDQNFVN 202

[Close]

BLAST of Carg22070-RA vs. TAIR 10
Match: AT5G42900.2 (cold regulated gene 27 )

HSP 1 Score: 59.3 bits (142), Expect = 5.7e-09
Identity = 67/214 (31.31%), Postives = 94/214 (43.93%), Query Frame = 0


Query: 30  RLNSESYSSSNTVDASEGDLPLMPSHHCFQLDAVQTHWTEEKHRLFLDSLEASFVQDLHR 89
           R + +S  SS+   + EG+     S +    + ++T WT EKH L+L S+EASFV  L  
Sbjct: 13  RFSDDSDDSSDDASSVEGE--TTSSMYSAGKEYMETEWTNEKHSLYLKSMEASFVDQL-- 72

Query: 90  YRHLRALSSKQKMRRKPIMPNISSSEVRQYIHSKSGSEFPISFLYSMVLQDASWQKANVG 149
           Y  L AL   +         N+S S       ++ GS    S     VL D  WQK NV 
Sbjct: 73  YNSLGALGKNE---------NVSES-------TRFGSGRKPSQEQFKVLHDGFWQKINVK 132

Query: 150 RTDPLLD--KTADSPAILEDVWINHF-PSAGNQCTLETTDVLEECGSCSDRSHIRNKTNS 209
           + +  ++     +S   L   WI H+ P    Q  +  TD  E        S+ +    S
Sbjct: 133 QPEHRINGRHGGNSHEFLRSPWIKHYKPLVKTQ--IPVTD--EPENQVVSSSNGKKGICS 192

Query: 210 RQSARSSQQIPPCCRDSHTI---VTEVSDQNFVD 238
             SA S +Q+    RD   I     EVSDQNFV+
Sbjct: 193 SGSASSLKQLSSHSRDHDQISVGEAEVSDQNFVN 202

The following BLAST results are available for this feature:

BLAST of Carg22070-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7011651.1	6.0e-154	100.00	hypothetical protein SDJN02_26557 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022952080.1	2.7e-138	92.83	uncharacterized protein LOC111454842 isoform X1 [Cucurbita moschata]	[more]
XP_022952081.1	7.2e-131	92.48	uncharacterized protein LOC111454842 isoform X2 [Cucurbita moschata]	[more]
XP_023553838.1	2.0e-128	87.10	uncharacterized protein LOC111811294 isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022972372.1	2.5e-128	87.46	uncharacterized protein LOC111470946 isoform X1 [Cucurbita maxima]	[more]

BLAST of Carg22070-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L8T7	8.0e-08	31.31	Cold-regulated protein 27 OS=Arabidopsis thaliana OX=3702 GN=COR27 PE=1 SV=1	[more]

BLAST of Carg22070-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GKR5	1.3e-138	92.83	uncharacterized protein LOC111454842 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1GJA3	3.5e-131	92.48	uncharacterized protein LOC111454842 isoform X2 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1I4M4	1.2e-128	87.46	uncharacterized protein LOC111470946 isoform X1 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1I9R8	2.3e-122	87.59	uncharacterized protein LOC111470946 isoform X2 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1GJF7	1.1e-113	81.00	uncharacterized protein LOC111454842 isoform X3 OS=Cucurbita moschata OX=3662 GN...	[more]

BLAST of Carg22070-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G42900.3	4.3e-09	31.46	cold regulated gene 27	[more]
AT5G42900.1	5.7e-09	31.31	cold regulated gene 27	[more]
AT5G42900.2	5.7e-09	31.31	cold regulated gene 27	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..48
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 196..218
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..45
None	No IPR available	PANTHER	PTHR33676:SF17	REGULATED GENE 27, PUTATIVE ISOFORM 2-RELATED	coord: 23..266
IPR044678	Cold-regulated protein 27/28	PANTHER	PTHR33676	COLD REGULATED PROTEIN 27	coord: 23..266

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg22070	Carg22070	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg22070-RA:1	Carg22070-RA:1	exon
Carg22070-RA:2	Carg22070-RA:2	exon
Carg22070-RA:3	Carg22070-RA:3	exon
Carg22070-RA:4	Carg22070-RA:4	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg22070-RA:five_prime_utr	Carg22070-RA:five_prime_utr	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg22070-RA:cds	Carg22070-RA:cds	CDS
Carg22070-RA:cds	Carg22070-RA:cds_2	CDS
Carg22070-RA:cds	Carg22070-RA:cds_3	CDS
Carg22070-RA:cds	Carg22070-RA:cds_4	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg22070-RA:three_prime_utr	Carg22070-RA:three_prime_utr	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg22070-RA	Carg22070-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042752	regulation of circadian rhythm
biological_process	GO:0009409	response to cold