Carg18054-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg18054-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Calcium-binding EF-hand family protein
Location	Carg_Chr11: 11911085 .. 11911432 (-)
Sequence length	348
RNA-Seq Expression	Carg18054-RA
Synteny	Carg18054-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGCGTAGAATTACTAGACAGCGCCACCGTAGTGAGCTTCGTGGAGGACGAGGAAGCCTTCGGCGCCGCCATTAGGGAGCGTTTCAGGCGCCTCGATGTGGACGGCGATGGCGCGCTGTCGTACACAGAGATGTTGAAGGAGTTTGGAAGCGATGAGGTTTGTTGTGCGTACGAAAGTGAGTCGAATGGAAAAGTTGATTTGGAGAAGTTCATGAGGGGAATGAAGAAGATGATGATGGCCATTGCTGAGGGATTAGGGTTCTTGCCGATTCATATGGCGCTGCAAAAAGATGGGTTGTTGATGAAAGCTGTTCAGAGAGAAGCCGCTGCCATGGTAGATGCTTGA

mRNA sequence

ATGAGCGTAGAATTACTAGACAGCGCCACCGTAGTGAGCTTCGTGGAGGACGAGGAAGCCTTCGGCGCCGCCATTAGGGAGCGTTTCAGGCGCCTCGATGTGGACGGCGATGGCGCGCTGTCGTACACAGAGATGTTGAAGGAGTTTGGAAGCGATGAGGTTTGTTGTGCGTACGAAAGTGAGTCGAATGGAAAAGTTGATTTGGAGAAGTTCATGAGGGGAATGAAGAAGATGATGATGGCCATTGCTGAGGGATTAGGGTTCTTGCCGATTCATATGGCGCTGCAAAAAGATGGGTTGTTGATGAAAGCTGTTCAGAGAGAAGCCGCTGCCATGGTAGATGCTTGA

Coding sequence (CDS)

ATGAGCGTAGAATTACTAGACAGCGCCACCGTAGTGAGCTTCGTGGAGGACGAGGAAGCCTTCGGCGCCGCCATTAGGGAGCGTTTCAGGCGCCTCGATGTGGACGGCGATGGCGCGCTGTCGTACACAGAGATGTTGAAGGAGTTTGGAAGCGATGAGGTTTGTTGTGCGTACGAAAGTGAGTCGAATGGAAAAGTTGATTTGGAGAAGTTCATGAGGGGAATGAAGAAGATGATGATGGCCATTGCTGAGGGATTAGGGTTCTTGCCGATTCATATGGCGCTGCAAAAAGATGGGTTGTTGATGAAAGCTGTTCAGAGAGAAGCCGCTGCCATGGTAGATGCTTGA

Protein sequence

MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKEFGSDEVCCAYESESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQKDGLLMKAVQREAAAMVDA

Homology

[Close]

BLAST of Carg18054-RA vs. NCBI nr
Match: KAG7022828.1 (hypothetical protein SDJN02_16564, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 223.0 bits (567), Expect = 1.3e-54
Identity = 115/115 (100.00%), Postives = 115/115 (100.00%), Query Frame = 0


Query: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKEFGSDEVCCAYES 60
           MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKEFGSDEVCCAYES
Sbjct: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKEFGSDEVCCAYES 60

Query: 61  ESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQKDGLLMKAVQREAAAMVDA 116
           ESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQKDGLLMKAVQREAAAMVDA
Sbjct: 61  ESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQKDGLLMKAVQREAAAMVDA 115

[Close]

BLAST of Carg18054-RA vs. NCBI nr
Match: XP_022930694.1 (uncharacterized protein LOC111437091 [Cucurbita moschata] >XP_023530313.1 uncharacterized protein LOC111792919 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 219.9 bits (559), Expect = 1.1e-53
Identity = 113/115 (98.26%), Postives = 114/115 (99.13%), Query Frame = 0


Query: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKEFGSDEVCCAYES 60
           MSVELLDSATVVSFVEDEEAFGAAIRERFR LDVDGDGALSYTEMLKEFGSDEVCCAYE+
Sbjct: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRHLDVDGDGALSYTEMLKEFGSDEVCCAYET 60

Query: 61  ESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQKDGLLMKAVQREAAAMVDA 116
           ESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQKDGLLMKAVQREAAAMVDA
Sbjct: 61  ESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQKDGLLMKAVQREAAAMVDA 115

[Close]

BLAST of Carg18054-RA vs. NCBI nr
Match: KAG6589126.1 (hypothetical protein SDJN03_17691, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 213.0 bits (541), Expect = 1.4e-51
Identity = 109/112 (97.32%), Postives = 110/112 (98.21%), Query Frame = 0


Query: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKEFGSDEVCCAYES 60
           MSVELLDSATVVSFVEDEEAFGAAIRERFR LDVDGDGALSYTEMLKEFGSDEVCC YE+
Sbjct: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRHLDVDGDGALSYTEMLKEFGSDEVCCVYET 60

Query: 61  ESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQKDGLLMKAVQREAAAM 113
           ESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQKDGLLMKAVQREAAAM
Sbjct: 61  ESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQKDGLLMKAVQREAAAM 112

[Close]

BLAST of Carg18054-RA vs. NCBI nr
Match: XP_038887184.1 (uncharacterized protein LOC120077371 [Benincasa hispida])

HSP 1 Score: 136.7 bits (343), Expect = 1.2e-28
Identity = 77/132 (58.33%), Postives = 89/132 (67.42%), Query Frame = 0


Query: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKE----------FG 60
           MSVELLD AT+V+FVEDEEAF    RE F  LD DGDG LSY EMLKE          FG
Sbjct: 1   MSVELLDGATIVNFVEDEEAFNGWTREHFNHLDTDGDGFLSYAEMLKELHSLRVSEADFG 60

Query: 61  SD-------------EVCCAYESESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQK 110
           +D              V   ++ +SNGKVD+++FM GMK MM+AIAEG+GFLPI MAL+K
Sbjct: 61  TDVKPDADDLSRLYGAVFGQFDRDSNGKVDVDEFMMGMKNMMLAIAEGMGFLPIQMALEK 120

[Close]

BLAST of Carg18054-RA vs. NCBI nr
Match: XP_008455505.1 (PREDICTED: uncharacterized protein LOC103495656 [Cucumis melo] >KAA0059136.1 uncharacterized protein E6C27_scaffold430G00460 [Cucumis melo var. makuwa] >TYK21611.1 uncharacterized protein E5676_scaffold150G00080 [Cucumis melo var. makuwa])

HSP 1 Score: 133.7 bits (335), Expect = 1.0e-27
Identity = 76/132 (57.58%), Postives = 89/132 (67.42%), Query Frame = 0


Query: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKEF----------- 60
           MSVELLD AT+V+FVEDEEAF    RE F RLD D DG LSY EMLKEF           
Sbjct: 1   MSVELLDGATIVNFVEDEEAFNGWTREHFTRLDTDRDGFLSYAEMLKEFYTLRVSEADFG 60

Query: 61  -----GSDEVCCAY-------ESESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQK 110
                 +DE+   Y       + +S+GKVD+++FM GMK MM+AIAEG+GFLPI MAL+K
Sbjct: 61  TDVKLDADELSQLYGAVFKQFDRDSDGKVDVDEFMMGMKNMMLAIAEGMGFLPIQMALEK 120

[Close]

BLAST of Carg18054-RA vs. ExPASy TrEMBL
Match: A0A6J1ES74 (uncharacterized protein LOC111437091 OS=Cucurbita moschata OX=3662 GN=LOC111437091 PE=4 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 5.4e-54
Identity = 113/115 (98.26%), Postives = 114/115 (99.13%), Query Frame = 0


Query: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKEFGSDEVCCAYES 60
           MSVELLDSATVVSFVEDEEAFGAAIRERFR LDVDGDGALSYTEMLKEFGSDEVCCAYE+
Sbjct: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRHLDVDGDGALSYTEMLKEFGSDEVCCAYET 60

Query: 61  ESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQKDGLLMKAVQREAAAMVDA 116
           ESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQKDGLLMKAVQREAAAMVDA
Sbjct: 61  ESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQKDGLLMKAVQREAAAMVDA 115

[Close]

BLAST of Carg18054-RA vs. ExPASy TrEMBL
Match: A0A5A7UY59 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold150G00080 PE=4 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 5.1e-28
Identity = 76/132 (57.58%), Postives = 89/132 (67.42%), Query Frame = 0


Query: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKEF----------- 60
           MSVELLD AT+V+FVEDEEAF    RE F RLD D DG LSY EMLKEF           
Sbjct: 1   MSVELLDGATIVNFVEDEEAFNGWTREHFTRLDTDRDGFLSYAEMLKEFYTLRVSEADFG 60

Query: 61  -----GSDEVCCAY-------ESESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQK 110
                 +DE+   Y       + +S+GKVD+++FM GMK MM+AIAEG+GFLPI MAL+K
Sbjct: 61  TDVKLDADELSQLYGAVFKQFDRDSDGKVDVDEFMMGMKNMMLAIAEGMGFLPIQMALEK 120

[Close]

BLAST of Carg18054-RA vs. ExPASy TrEMBL
Match: A0A1S3C0M6 (uncharacterized protein LOC103495656 OS=Cucumis melo OX=3656 GN=LOC103495656 PE=4 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 5.1e-28
Identity = 76/132 (57.58%), Postives = 89/132 (67.42%), Query Frame = 0


Query: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKEF----------- 60
           MSVELLD AT+V+FVEDEEAF    RE F RLD D DG LSY EMLKEF           
Sbjct: 1   MSVELLDGATIVNFVEDEEAFNGWTREHFTRLDTDRDGFLSYAEMLKEFYTLRVSEADFG 60

Query: 61  -----GSDEVCCAY-------ESESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQK 110
                 +DE+   Y       + +S+GKVD+++FM GMK MM+AIAEG+GFLPI MAL+K
Sbjct: 61  TDVKLDADELSQLYGAVFKQFDRDSDGKVDVDEFMMGMKNMMLAIAEGMGFLPIQMALEK 120

[Close]

BLAST of Carg18054-RA vs. ExPASy TrEMBL
Match: A0A6J1C2T1 (uncharacterized protein LOC111007741 OS=Momordica charantia OX=3673 GN=LOC111007741 PE=4 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 1.9e-27
Identity = 73/131 (55.73%), Postives = 90/131 (68.70%), Query Frame = 0


Query: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKE----------FG 60
           MSVE+LDSAT+++FVEDEEAFGA IRERFR LD D DG LSY EML+E          FG
Sbjct: 1   MSVEILDSATIINFVEDEEAFGAWIRERFRHLDADRDGVLSYAEMLQELHSLRVYETHFG 60

Query: 61  SD-------------EVCCAYESESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQK 109
            D              V   ++  SNG+VDLE+FM G K++M+AIAEG+GFLP+ MAL++
Sbjct: 61  VDAKPDPDELSRLYGAVFMQFDRNSNGEVDLEEFMAGTKRIMVAIAEGMGFLPLQMALEE 120

[Close]

BLAST of Carg18054-RA vs. ExPASy TrEMBL
Match: A0A438IL73 (EF-hand domain-containing protein OS=Vitis vinifera OX=29760 GN=CK203_026186 PE=4 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 7.6e-24
Identity = 63/112 (56.25%), Postives = 80/112 (71.43%), Query Frame = 0


Query: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKEFGSDEVCCAYES 60
           MSVE+LD AT+VSFVED+EAF   I  RF  LD + DG LSY EMLKEF S      ++ 
Sbjct: 1   MSVEVLDGATIVSFVEDKEAFNGLIHHRFSNLDTNHDGFLSYPEMLKEFQS----LRFDH 60

Query: 61  ESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQKDGLLMKAVQREAAAM 113
           ++NG VDLE+F +  K+MM+A+A GLGFLP+ M L++D  L KAV+REA  M
Sbjct: 61  DANGAVDLEEFRKETKQMMLAMANGLGFLPVQMVLEEDSFLKKAVEREAMKM 108

[Close]

BLAST of Carg18054-RA vs. TAIR 10
Match: AT2G44310.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 81.6 bits (200), Expect = 4.4e-16
Identity = 56/140 (40.00%), Postives = 76/140 (54.29%), Query Frame = 0


Query: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKEFGS--------- 60
           M V L+D +TV SFV+DEE F  ++ ERF  LD++ DG LS +E+ K F S         
Sbjct: 1   MGVVLIDGSTVRSFVDDEEQFKKSVDERFAALDLNKDGVLSRSELRKAFESMRLLESHFG 60

Query: 61  -------DEVCCAYES-------ESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMAL-- 116
                  DE+   Y+S       + +G VDLE+F   MKK+++AIA+GLG  PI M L  
Sbjct: 61  VDVVTPQDELTNLYDSIFEKFDTDQSGSVDLEEFRSEMKKIVLAIADGLGSCPITMVLDD 120

[Close]

BLAST of Carg18054-RA vs. TAIR 10
Match: AT1G54530.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 48.5 bits (114), Expect = 4.1e-06
Identity = 32/119 (26.89%), Postives = 62/119 (52.10%), Query Frame = 0


Query: 5   LLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKEFGS-----DEVCCAYE 64
           ++ S T++ F+ D ++F +   + F+ LD+D +G LS +E+ +   +      EV    E
Sbjct: 5   VITSKTLIGFLSDTKSFESITNDYFQILDLDKNGMLSPSELRQGLNNVVAVESEVAPGDE 64

Query: 65  SESNGKVDLEKFMRGM---------KKMMMAIAEGLGFLPIHMALQKDGLLMKAVQREA 110
           +++      E+F   +          +++ A+A G+G  P+ M +  DGL+MKAV  E+
Sbjct: 65  TDNVYNAIFERFGEDLVPKNFRDLIAEILTAMARGIGNSPVIMVVHNDGLIMKAVLHES 123

[Close]

BLAST of Carg18054-RA vs. TAIR 10
Match: AT4G38810.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 42.7 bits (99), Expect = 2.3e-04
Identity = 32/116 (27.59%), Postives = 56/116 (48.28%), Query Frame = 0


Query: 4   ELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEM---LKEFGS--------- 63
           ++LD + +V  VE+E+ F   + ++F++LD D DG LS TE+   + + G+         
Sbjct: 13  QVLDGSDIVELVENEKVFDKFVEQKFQQLDQDEDGKLSVTELQPAVADIGAALGLPAQGT 72

Query: 64  --------DEVCCAYESESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQKDG 100
                    EV   +   S  KV   +F   +  +++ +A GL   PI + L+ DG
Sbjct: 73  SPDSDHIYSEVLNEFTHGSQEKVSKTEFKEVLSDILLGMAAGLKRDPI-VILRMDG 127

The following BLAST results are available for this feature:

BLAST of Carg18054-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7022828.1	1.3e-54	100.00	hypothetical protein SDJN02_16564, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022930694.1	1.1e-53	98.26	uncharacterized protein LOC111437091 [Cucurbita moschata] >XP_023530313.1 unchar...	[more]
KAG6589126.1	1.4e-51	97.32	hypothetical protein SDJN03_17691, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_038887184.1	1.2e-28	58.33	uncharacterized protein LOC120077371 [Benincasa hispida]	[more]
XP_008455505.1	1.0e-27	57.58	PREDICTED: uncharacterized protein LOC103495656 [Cucumis melo] >KAA0059136.1 unc...	[more]

BLAST of Carg18054-RA vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg18054-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1ES74	5.4e-54	98.26	uncharacterized protein LOC111437091 OS=Cucurbita moschata OX=3662 GN=LOC1114370...	[more]
A0A5A7UY59	5.1e-28	57.58	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A1S3C0M6	5.1e-28	57.58	uncharacterized protein LOC103495656 OS=Cucumis melo OX=3656 GN=LOC103495656 PE=...	[more]
A0A6J1C2T1	1.9e-27	55.73	uncharacterized protein LOC111007741 OS=Momordica charantia OX=3673 GN=LOC111007...	[more]
A0A438IL73	7.6e-24	56.25	EF-hand domain-containing protein OS=Vitis vinifera OX=29760 GN=CK203_026186 PE=...	[more]

BLAST of Carg18054-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G44310.1	4.4e-16	40.00	Calcium-binding EF-hand family protein	[more]
AT1G54530.1	4.1e-06	26.89	Calcium-binding EF hand family protein	[more]
AT4G38810.2	2.3e-04	27.59	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.10.238.10		coord: 11..109 e-value: 8.4E-8 score: 33.7
None	No IPR available	PANTHER	PTHR34574	CALCIUM-BINDING EF-HAND FAMILY PROTEIN-RELATED	coord: 1..50
None	No IPR available	PANTHER	PTHR34574	CALCIUM-BINDING EF-HAND FAMILY PROTEIN-RELATED	coord: 54..110
None	No IPR available	PANTHER	PTHR34574:SF12	CALCIUM-BINDING EF HAND FAMILY PROTEIN	coord: 54..110 coord: 1..50
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 33..45
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 20..55 score: 8.711163
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 19..86

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg18054	Carg18054	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg18054-RA:1	Carg18054-RA:1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg18054-RA:cds	Carg18054-RA:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg18054-RA	Carg18054-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding