Carg18054 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg18054
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionCalcium-binding EF-hand family protein
LocationCarg_Chr11: 11911085 .. 11911432 (-)
RNA-Seq ExpressionCarg18054
SyntenyCarg18054
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCGTAGAATTACTAGACAGCGCCACCGTAGTGAGCTTCGTGGAGGACGAGGAAGCCTTCGGCGCCGCCATTAGGGAGCGTTTCAGGCGCCTCGATGTGGACGGCGATGGCGCGCTGTCGTACACAGAGATGTTGAAGGAGTTTGGAAGCGATGAGGTTTGTTGTGCGTACGAAAGTGAGTCGAATGGAAAAGTTGATTTGGAGAAGTTCATGAGGGGAATGAAGAAGATGATGATGGCCATTGCTGAGGGATTAGGGTTCTTGCCGATTCATATGGCGCTGCAAAAAGATGGGTTGTTGATGAAAGCTGTTCAGAGAGAAGCCGCTGCCATGGTAGATGCTTGA

mRNA sequence

ATGAGCGTAGAATTACTAGACAGCGCCACCGTAGTGAGCTTCGTGGAGGACGAGGAAGCCTTCGGCGCCGCCATTAGGGAGCGTTTCAGGCGCCTCGATGTGGACGGCGATGGCGCGCTGTCGTACACAGAGATGTTGAAGGAGTTTGGAAGCGATGAGGTTTGTTGTGCGTACGAAAGTGAGTCGAATGGAAAAGTTGATTTGGAGAAGTTCATGAGGGGAATGAAGAAGATGATGATGGCCATTGCTGAGGGATTAGGGTTCTTGCCGATTCATATGGCGCTGCAAAAAGATGGGTTGTTGATGAAAGCTGTTCAGAGAGAAGCCGCTGCCATGGTAGATGCTTGA

Coding sequence (CDS)

ATGAGCGTAGAATTACTAGACAGCGCCACCGTAGTGAGCTTCGTGGAGGACGAGGAAGCCTTCGGCGCCGCCATTAGGGAGCGTTTCAGGCGCCTCGATGTGGACGGCGATGGCGCGCTGTCGTACACAGAGATGTTGAAGGAGTTTGGAAGCGATGAGGTTTGTTGTGCGTACGAAAGTGAGTCGAATGGAAAAGTTGATTTGGAGAAGTTCATGAGGGGAATGAAGAAGATGATGATGGCCATTGCTGAGGGATTAGGGTTCTTGCCGATTCATATGGCGCTGCAAAAAGATGGGTTGTTGATGAAAGCTGTTCAGAGAGAAGCCGCTGCCATGGTAGATGCTTGA

Protein sequence

MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKEFGSDEVCCAYESESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQKDGLLMKAVQREAAAMVDA
Homology
BLAST of Carg18054 vs. NCBI nr
Match: KAG7022828.1 (hypothetical protein SDJN02_16564, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 223.0 bits (567), Expect = 1.3e-54
Identity = 115/115 (100.00%), Postives = 115/115 (100.00%), Query Frame = 0

Query: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKEFGSDEVCCAYES 60
           MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKEFGSDEVCCAYES
Sbjct: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKEFGSDEVCCAYES 60

Query: 61  ESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQKDGLLMKAVQREAAAMVDA 116
           ESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQKDGLLMKAVQREAAAMVDA
Sbjct: 61  ESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQKDGLLMKAVQREAAAMVDA 115

BLAST of Carg18054 vs. NCBI nr
Match: XP_022930694.1 (uncharacterized protein LOC111437091 [Cucurbita moschata] >XP_023530313.1 uncharacterized protein LOC111792919 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 219.9 bits (559), Expect = 1.1e-53
Identity = 113/115 (98.26%), Postives = 114/115 (99.13%), Query Frame = 0

Query: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKEFGSDEVCCAYES 60
           MSVELLDSATVVSFVEDEEAFGAAIRERFR LDVDGDGALSYTEMLKEFGSDEVCCAYE+
Sbjct: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRHLDVDGDGALSYTEMLKEFGSDEVCCAYET 60

Query: 61  ESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQKDGLLMKAVQREAAAMVDA 116
           ESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQKDGLLMKAVQREAAAMVDA
Sbjct: 61  ESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQKDGLLMKAVQREAAAMVDA 115

BLAST of Carg18054 vs. NCBI nr
Match: KAG6589126.1 (hypothetical protein SDJN03_17691, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 213.0 bits (541), Expect = 1.4e-51
Identity = 109/112 (97.32%), Postives = 110/112 (98.21%), Query Frame = 0

Query: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKEFGSDEVCCAYES 60
           MSVELLDSATVVSFVEDEEAFGAAIRERFR LDVDGDGALSYTEMLKEFGSDEVCC YE+
Sbjct: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRHLDVDGDGALSYTEMLKEFGSDEVCCVYET 60

Query: 61  ESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQKDGLLMKAVQREAAAM 113
           ESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQKDGLLMKAVQREAAAM
Sbjct: 61  ESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQKDGLLMKAVQREAAAM 112

BLAST of Carg18054 vs. NCBI nr
Match: XP_038887184.1 (uncharacterized protein LOC120077371 [Benincasa hispida])

HSP 1 Score: 136.7 bits (343), Expect = 1.2e-28
Identity = 77/132 (58.33%), Postives = 89/132 (67.42%), Query Frame = 0

Query: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKE----------FG 60
           MSVELLD AT+V+FVEDEEAF    RE F  LD DGDG LSY EMLKE          FG
Sbjct: 1   MSVELLDGATIVNFVEDEEAFNGWTREHFNHLDTDGDGFLSYAEMLKELHSLRVSEADFG 60

Query: 61  SD-------------EVCCAYESESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQK 110
           +D              V   ++ +SNGKVD+++FM GMK MM+AIAEG+GFLPI MAL+K
Sbjct: 61  TDVKPDADDLSRLYGAVFGQFDRDSNGKVDVDEFMMGMKNMMLAIAEGMGFLPIQMALEK 120

BLAST of Carg18054 vs. NCBI nr
Match: XP_008455505.1 (PREDICTED: uncharacterized protein LOC103495656 [Cucumis melo] >KAA0059136.1 uncharacterized protein E6C27_scaffold430G00460 [Cucumis melo var. makuwa] >TYK21611.1 uncharacterized protein E5676_scaffold150G00080 [Cucumis melo var. makuwa])

HSP 1 Score: 133.7 bits (335), Expect = 1.0e-27
Identity = 76/132 (57.58%), Postives = 89/132 (67.42%), Query Frame = 0

Query: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKEF----------- 60
           MSVELLD AT+V+FVEDEEAF    RE F RLD D DG LSY EMLKEF           
Sbjct: 1   MSVELLDGATIVNFVEDEEAFNGWTREHFTRLDTDRDGFLSYAEMLKEFYTLRVSEADFG 60

Query: 61  -----GSDEVCCAY-------ESESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQK 110
                 +DE+   Y       + +S+GKVD+++FM GMK MM+AIAEG+GFLPI MAL+K
Sbjct: 61  TDVKLDADELSQLYGAVFKQFDRDSDGKVDVDEFMMGMKNMMLAIAEGMGFLPIQMALEK 120

BLAST of Carg18054 vs. ExPASy TrEMBL
Match: A0A6J1ES74 (uncharacterized protein LOC111437091 OS=Cucurbita moschata OX=3662 GN=LOC111437091 PE=4 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 5.4e-54
Identity = 113/115 (98.26%), Postives = 114/115 (99.13%), Query Frame = 0

Query: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKEFGSDEVCCAYES 60
           MSVELLDSATVVSFVEDEEAFGAAIRERFR LDVDGDGALSYTEMLKEFGSDEVCCAYE+
Sbjct: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRHLDVDGDGALSYTEMLKEFGSDEVCCAYET 60

Query: 61  ESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQKDGLLMKAVQREAAAMVDA 116
           ESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQKDGLLMKAVQREAAAMVDA
Sbjct: 61  ESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQKDGLLMKAVQREAAAMVDA 115

BLAST of Carg18054 vs. ExPASy TrEMBL
Match: A0A5A7UY59 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold150G00080 PE=4 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 5.1e-28
Identity = 76/132 (57.58%), Postives = 89/132 (67.42%), Query Frame = 0

Query: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKEF----------- 60
           MSVELLD AT+V+FVEDEEAF    RE F RLD D DG LSY EMLKEF           
Sbjct: 1   MSVELLDGATIVNFVEDEEAFNGWTREHFTRLDTDRDGFLSYAEMLKEFYTLRVSEADFG 60

Query: 61  -----GSDEVCCAY-------ESESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQK 110
                 +DE+   Y       + +S+GKVD+++FM GMK MM+AIAEG+GFLPI MAL+K
Sbjct: 61  TDVKLDADELSQLYGAVFKQFDRDSDGKVDVDEFMMGMKNMMLAIAEGMGFLPIQMALEK 120

BLAST of Carg18054 vs. ExPASy TrEMBL
Match: A0A1S3C0M6 (uncharacterized protein LOC103495656 OS=Cucumis melo OX=3656 GN=LOC103495656 PE=4 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 5.1e-28
Identity = 76/132 (57.58%), Postives = 89/132 (67.42%), Query Frame = 0

Query: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKEF----------- 60
           MSVELLD AT+V+FVEDEEAF    RE F RLD D DG LSY EMLKEF           
Sbjct: 1   MSVELLDGATIVNFVEDEEAFNGWTREHFTRLDTDRDGFLSYAEMLKEFYTLRVSEADFG 60

Query: 61  -----GSDEVCCAY-------ESESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQK 110
                 +DE+   Y       + +S+GKVD+++FM GMK MM+AIAEG+GFLPI MAL+K
Sbjct: 61  TDVKLDADELSQLYGAVFKQFDRDSDGKVDVDEFMMGMKNMMLAIAEGMGFLPIQMALEK 120

BLAST of Carg18054 vs. ExPASy TrEMBL
Match: A0A6J1C2T1 (uncharacterized protein LOC111007741 OS=Momordica charantia OX=3673 GN=LOC111007741 PE=4 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 1.9e-27
Identity = 73/131 (55.73%), Postives = 90/131 (68.70%), Query Frame = 0

Query: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKE----------FG 60
           MSVE+LDSAT+++FVEDEEAFGA IRERFR LD D DG LSY EML+E          FG
Sbjct: 1   MSVEILDSATIINFVEDEEAFGAWIRERFRHLDADRDGVLSYAEMLQELHSLRVYETHFG 60

Query: 61  SD-------------EVCCAYESESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQK 109
            D              V   ++  SNG+VDLE+FM G K++M+AIAEG+GFLP+ MAL++
Sbjct: 61  VDAKPDPDELSRLYGAVFMQFDRNSNGEVDLEEFMAGTKRIMVAIAEGMGFLPLQMALEE 120

BLAST of Carg18054 vs. ExPASy TrEMBL
Match: A0A438IL73 (EF-hand domain-containing protein OS=Vitis vinifera OX=29760 GN=CK203_026186 PE=4 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 7.6e-24
Identity = 63/112 (56.25%), Postives = 80/112 (71.43%), Query Frame = 0

Query: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKEFGSDEVCCAYES 60
           MSVE+LD AT+VSFVED+EAF   I  RF  LD + DG LSY EMLKEF S      ++ 
Sbjct: 1   MSVEVLDGATIVSFVEDKEAFNGLIHHRFSNLDTNHDGFLSYPEMLKEFQS----LRFDH 60

Query: 61  ESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQKDGLLMKAVQREAAAM 113
           ++NG VDLE+F +  K+MM+A+A GLGFLP+ M L++D  L KAV+REA  M
Sbjct: 61  DANGAVDLEEFRKETKQMMLAMANGLGFLPVQMVLEEDSFLKKAVEREAMKM 108

BLAST of Carg18054 vs. TAIR 10
Match: AT2G44310.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 81.6 bits (200), Expect = 4.4e-16
Identity = 56/140 (40.00%), Postives = 76/140 (54.29%), Query Frame = 0

Query: 1   MSVELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKEFGS--------- 60
           M V L+D +TV SFV+DEE F  ++ ERF  LD++ DG LS +E+ K F S         
Sbjct: 1   MGVVLIDGSTVRSFVDDEEQFKKSVDERFAALDLNKDGVLSRSELRKAFESMRLLESHFG 60

Query: 61  -------DEVCCAYES-------ESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMAL-- 116
                  DE+   Y+S       + +G VDLE+F   MKK+++AIA+GLG  PI M L  
Sbjct: 61  VDVVTPQDELTNLYDSIFEKFDTDQSGSVDLEEFRSEMKKIVLAIADGLGSCPITMVLDD 120

BLAST of Carg18054 vs. TAIR 10
Match: AT1G54530.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 48.5 bits (114), Expect = 4.1e-06
Identity = 32/119 (26.89%), Postives = 62/119 (52.10%), Query Frame = 0

Query: 5   LLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEMLKEFGS-----DEVCCAYE 64
           ++ S T++ F+ D ++F +   + F+ LD+D +G LS +E+ +   +      EV    E
Sbjct: 5   VITSKTLIGFLSDTKSFESITNDYFQILDLDKNGMLSPSELRQGLNNVVAVESEVAPGDE 64

Query: 65  SESNGKVDLEKFMRGM---------KKMMMAIAEGLGFLPIHMALQKDGLLMKAVQREA 110
           +++      E+F   +          +++ A+A G+G  P+ M +  DGL+MKAV  E+
Sbjct: 65  TDNVYNAIFERFGEDLVPKNFRDLIAEILTAMARGIGNSPVIMVVHNDGLIMKAVLHES 123

BLAST of Carg18054 vs. TAIR 10
Match: AT4G38810.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 42.7 bits (99), Expect = 2.3e-04
Identity = 32/116 (27.59%), Postives = 56/116 (48.28%), Query Frame = 0

Query: 4   ELLDSATVVSFVEDEEAFGAAIRERFRRLDVDGDGALSYTEM---LKEFGS--------- 63
           ++LD + +V  VE+E+ F   + ++F++LD D DG LS TE+   + + G+         
Sbjct: 13  QVLDGSDIVELVENEKVFDKFVEQKFQQLDQDEDGKLSVTELQPAVADIGAALGLPAQGT 72

Query: 64  --------DEVCCAYESESNGKVDLEKFMRGMKKMMMAIAEGLGFLPIHMALQKDG 100
                    EV   +   S  KV   +F   +  +++ +A GL   PI + L+ DG
Sbjct: 73  SPDSDHIYSEVLNEFTHGSQEKVSKTEFKEVLSDILLGMAAGLKRDPI-VILRMDG 127

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7022828.11.3e-54100.00hypothetical protein SDJN02_16564, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022930694.11.1e-5398.26uncharacterized protein LOC111437091 [Cucurbita moschata] >XP_023530313.1 unchar... [more]
KAG6589126.11.4e-5197.32hypothetical protein SDJN03_17691, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_038887184.11.2e-2858.33uncharacterized protein LOC120077371 [Benincasa hispida][more]
XP_008455505.11.0e-2757.58PREDICTED: uncharacterized protein LOC103495656 [Cucumis melo] >KAA0059136.1 unc... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1ES745.4e-5498.26uncharacterized protein LOC111437091 OS=Cucurbita moschata OX=3662 GN=LOC1114370... [more]
A0A5A7UY595.1e-2857.58Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3C0M65.1e-2857.58uncharacterized protein LOC103495656 OS=Cucumis melo OX=3656 GN=LOC103495656 PE=... [more]
A0A6J1C2T11.9e-2755.73uncharacterized protein LOC111007741 OS=Momordica charantia OX=3673 GN=LOC111007... [more]
A0A438IL737.6e-2456.25EF-hand domain-containing protein OS=Vitis vinifera OX=29760 GN=CK203_026186 PE=... [more]
Match NameE-valueIdentityDescription
AT2G44310.14.4e-1640.00Calcium-binding EF-hand family protein [more]
AT1G54530.14.1e-0626.89Calcium-binding EF hand family protein [more]
AT4G38810.22.3e-0427.59Calcium-binding EF-hand family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.238.10coord: 11..109
e-value: 8.4E-8
score: 33.7
NoneNo IPR availablePANTHERPTHR34574CALCIUM-BINDING EF-HAND FAMILY PROTEIN-RELATEDcoord: 1..50
NoneNo IPR availablePANTHERPTHR34574CALCIUM-BINDING EF-HAND FAMILY PROTEIN-RELATEDcoord: 54..110
NoneNo IPR availablePANTHERPTHR34574:SF12CALCIUM-BINDING EF HAND FAMILY PROTEINcoord: 54..110
coord: 1..50
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 33..45
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 20..55
score: 8.711163
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 19..86

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg18054-RACarg18054-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding