Carg12755-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg12755-RA
TypemRNA
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionBeta-glucosidase
LocationCarg_Chr09: 4846117 .. 4852011 (-)
Sequence length1011
RNA-Seq ExpressionCarg12755-RA
SyntenyCarg12755-RA
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGAACAAGAACAACAAGAAATCAAAAGATCAGATTTTCCCGACCATTTCTTGTTCGGAACTGCCACTTCCTCGTACCAGGCACTGATTTCTCTCATCTTCTTCATCTTCTTCGTTTCCATTTCTGGTTTTGAAGCTTTTAGGGCACTTTATTGTACAGATCGAAGGAGCTTTTCTTGAAGATGGAAGGGGAACGAGTAACTGGGATGCGTTTTCCCACATTCCAGGTCTGACCCACTTCCTAATTTCACTTTCCCATCAAAACCCACTTCCCCGATTTGCGTATAACTTTCGTTATGATTCGGAATTTTTGAGATGAGGTTGGGTTTGATTTGCAGGAAGATATTGAGCTGATGCATTCCATGGGGATGAATGCTTATCGGTTTTCCATTTCCTGGACCCGAATTTTGCCAAGTAAGCTCCCCTCCTTTTCTCATTACCCAATTAGTGTAAAAAATTTCAAAAACCGGATAGACTTCCTCTCGTTAGGTTTCGGTGTAACAACACAAGCCCACAGCTAGCAGATATTGTCGTGTTTCTGCTTGTCCCACCGGCTTCCCTTCAAAGTTTTTAAAAATACATCTGTTAAGAAGAGGTTTCCATATCATTCTAGAGAAGGTTTCGTTCTCATCCCCGACCGACGTGGGATCTCACAATCCACCCCTTCTAGGCCAGCGTCCTTGCACCTCGGGTCCACCCCCAAAGTTTTCACACCCTTCTAAAAGATGTTTTGTACTCCTCCCCAACCGATGTGAGATCTCACAATCCACCCCCTTCTAGGTCAGCGTCCGTGCACCTCTAGTCCTCCCCCGAAGTTTTCACACCCTTCTAAAGGATGTCTCGTTCTCCTCCCCAACTGATGTGTAATCTCACAATCCATCCCCTTCTGAGCCAGTGTCCTTGCACCTTGGGTCCACCCCCCTTCGAGGCTCAATCTACTCGCTAGCACATCGCTCGATGTCTAGCTCTGATACCATTTATAATAGCCTAAACCCACTATTGTCCTATTTCGGCTTTCCCTTTCGACTTTCCTTTCAGGTTTTTAGATCACACCTACTAGTAAAAGGTTTTTACACACTTTTTTTTGGGATAAAATTAAAATTAAAAAAAACAAAATTATAATTTAATTGGTTAACAAAATTATTATTTAAAAAAAAAGAAATTAGGTTAATATTTATTTTTTAATTTATTGACGTTAGCATTTGTTTATTTGTCAAGAAGAAAATGACAATTAGGAATAATAATTAAATACAATAAAGTCTACATTTGACTATTATTTAATGTGAGGAATGTGCCTTACCAAATAATTAAAAAAAAATTAATATATGCTTTTTAAACGAATCATAATCTTTATTTATTTATTTATTATTATTATTATTTGGAAATGCAATCTATCTCAAAGATTTTATATATATATAAAAATATATTAAAATATTTAAAATGCGATCATAATCTTTAATCATTGATACGAATAATAAAAATTATTGAAAGATTATATTAATAATTTAATTTTGTAATAATAATAATAATAATATAGGAGGACGATTTGGTAAAATCAACAGAAGAGGGATCTCTTTCTACAATAAAGTAATTGATCGTTTGTTGCTAAGAGGTACCTCTCCTATTTCCTTTTGTTACTTTTACCCCTTTTTTTAGGGTTAATTACAATTTTAGTTTTAATTTATCTATAATTATGAATTGGGCAGGCATAGAGCCTTTTGTGACCATTCACCATCATGACCTCCCTGATGAGCTGGACAAAAGATATGGAAGTTGGATGAGCTCTCAAATGCAGTAATTTCTCCCCCTTTTTGTCTATTTTAATACTCGATGACTAGATAAATTCGGTGGATTCGGGTTGGGTTATGAATTTATTTTGGTTAGATCGGATCGGATAAATGAAAATTTTATGGGTTGAGTTGGTTCGTGGGTTTCTGTATGTGAGATCTTACGTTGGCCGGAGAGTGAACGAAACATTTCTTATAATGGTGTGGAAATCTCTCTGTAGTAGACGCGTTTTAAAAGGGAGGCTGATAGGGATATGTAACGAGCCAAAACGAACAATATCTATTGGCGGTGGGCTTGAGCTATTACAAATGATATCAGAGTCAAACATTAAGTGGTGTGTCAGCGAGGATGCTGGACTCCAAGGGGGTGGATTGTGAGATCCCACATTAGTTGGAGAAGGGAACGAAACATTTCTTATAATGGTGTGGAAACCTCTTTATAGTATATGCGTTTTAAAACTGTGAGGTTGATGGCAATATGTAACTCTCCTCATAGACACTAAACTGCGTGTCAGCGAGGACACTGGAGCTCAAGGGGTGGATTGTGAGATCCCACGTTAGTTAGAGAGAGGAATGAAACATTCCTTATAATGGTGTGGAAACCTCTGTATAGTAGACGCGTTTTAAAACTGTGAGGTTGATGGCAATACGTAACTCTCCAAAGTAGACAATATCTACTACGGATGGACTTGGGTTATTACAAACGGTATCATAGCCAAAACTAAGTGATGTATCAGCGGGACGCTGGACCCCAAGAGAGTGGATTGTGAGATCCCACATTAGTTGGAGAGGGGAACGAAACATTCCTTATAATGACATGGAAACCTATCCCTAGTAGAGTTTTAAAATTGTGAAGTTGACGGCGACACGTAATGCTCCAAAGCGGACAATATTTACTAACGATGGGTTTGGGCGGTTACACCCTTAATTTTTTTTTGTTTTGATTGAAAAAATTTACGATTCAAATCGTTGGGTTGGGTCTAAAAAATAACACGACCTGACCCGACCCAACCCATGAACACCTCTGCTCCTTTTAAGCTTTATTTGTGGGTGTCAACTAACACAATGAGGACAATCCAATGATTTATTGTTTTAGGGAAGATTTTGTGTATTTTGCACAAATATGCTTTGAAGAGTTCGGAGACAGAGTGAAGCATTGGATAACCATAAACGAGCCAAATTTGGTGGTATTAATGGCTTATATAAAAGGAGTTTATCCACCGGCTCACTGCTCGCCACCTTTTGGAAATTGCTCGGTTGGCAACTCCGATATCGAGCCTCTCATCGTCATGCACAACATGTTGCTTGCCCATGCCAAGGCCGTTCGACTTTATCGCACCCATTTTCAAGTAGCATTCTTCTCATCCTCTCCTTTTATTCATAATATGTTTTTAAAAAATTGTAATAATTTTATTAAATTCGAGCTTAGGATTAGTTAAAACAGTTATAAAATTATATGATTCATTAATTTATATATAACTTATTTGTAGGGCAAACAAGGCGGATCCATAGGCATAGTGGCATTTGGACACATGTATGAACCATTCACCGACAATGAATTGGACAGACAAGCTGCGGACAGAGCCTTGATTTTCAACTTTGCTTGGTCAGTTCTTTTTCCTTTTCTTTTGATATACCATGGTGTCGGAATATGGTGTCGGAATATGTAACAGTCAAGCTCACCGCTAGCAGAGATTGTCCTCTTTAAAATTTTCTTCTCGATTTTTCCCGCCTAAGGTTTCCACACTAGGTATTTCGTTCTCCTCCCCAACCCATGTGAGATATCACAATCCACCCCATTAGGGTCCAAACCAATGTGAGATATCACAATCCGTCCCTTTCAGGGCGAGCGTAAGGTTTCCACACCCTTATAAAGGGTGTTTCGTTCTCCTCCCCAACCAATGTAGGATATTGTCCTCCCCCTTCAGGACCCAGCGTCCAATGTCTTCACTGGCACTCTTCCTTTCTCCAATCGATGTGGTACCATCACCAAATCCACCTCCTTCGGTCACAGCGTCCTTATTGGCATACCGCCTCGTGCTACTCCCTTCAAGGCCCAGCGTTATAAGGGTGTTTCGTTCTCCTCCCCAACCAATGTGGGATATTGTCCACCTCCTTCAGGACCCAGTGTCCAACGTCCTCACTGACACTCGTTCCTTTCTCCATTCAATGTGGGACCACCACCAAGTCCACCCCCTTTCAGGGCCATCGTCTTTACTAGCACACCACCTCGTGTCTACCCCCTTGGGGGAACAGTCTACTCGCCGACAAATTACCTAGTGTCTAGCTCTGATACCATTTGTAACAACTCAAGCCCACCGTAGACTTTTCCTTTCGAACTTCCCCTTGAGACTTTAAAACACGTCTGTCAGGGAAAGGATGTTTCGTTCTCCTCCCAACTAATGTGGGATATAACAAAACATGCTATAATCGACTAAATTTAGTGAAGAAAACATGAACAGGGTATACGATCCGATCGTGTACGGAGATTATCCAAAGGAAATGCAAGCAATATTAGGAAGCCAACTTCCACGCTTCTCAAAGGCAGAGAAAAAGGTTGTAAAGGGAAGTTTGGACTACATTTATGTGAACCATTACACCACACTTTACACAAAGGACTGCCTTCATTCTATCTGCTCCGACGATGCAAATCGTCCAATTAAAGGCTTCCTCGACACCACCGGCTACCGGGACGGTGTTTCCATTGGTGACCCAGTATGCTCTTTCCCCCCTTTATCTTCTTGTGTTTATAAATACAACATGTGAGTGTGTAAATGATAAAGTTACCCTTTTTACATGACAGACTGGAGTTGACAGATTCTTTGTTGTTCCAAGAGGTTTGGAAAAGATCATTAATTATATCAAAGAAAGGTATCCCGACAACCCCATTTATGTCACTGAAAATGGTAAGTTGAGCTAAGTTTATCACATGGGTATGATTTAAATTTTTAATTTAGGTTTTAAGATATTGAAAATTTTGTTTTTTTTCAAGGTTACTCTGCACCGTCTAGTGACGGTAACAACGTTGAAGACATGGTAAATGACACGAAACGAGTCAATTATCACAAGAACTACCTCGCTTCCTTGGCTAAAGCAATGAGGTAAATAAACAACCTATTTACCTTCTTCATACAACCCCGTTATGTTTTTCATCTAGATTTCGCGTATATATATATTTAAAATATTTTCTATTTTTATTAAATCTGGGTAGGGTTGTTCATACGATCCAATGTCCCAGGTTGAGTATTTGGAAAACAAAAAATTTGATTTGGTTCGTGAGTCAAAACTTTTTTTAATTAGGTCAACCCGACCCACCAGAAATAGGGTTCACAACCCAACTCTATTCTACTTTTAAAATTTAAAATTGTTACGAAGATTAAAAATAATTAACATTTATAACGATGAAAGTGATATGTTGTGTTTGTAAATGTTTATATTTGAATTTTATGAGATTTTAGCTATTACACCTATCAAGAATAAATAGATTTTTTAAAAATAATTAAAAAAAATGAATAACTCAATGACCCGACCCAAAACTACGTCCAAACACACTACTAACGGATATTGTCCGTTTTGACCCGTTACATATCGTTATAAGCCTCACAATTTTAAAATGCGTCTACCAGGGAGAAGTTTCTATGTCCTTATAATAAATATTTCGTTTTACTCTCTAACCCACGTGGGATTTCATACATTGTGAACTCTATTCAAGTCATTCGGGTCATCAACCCAACCAACCTAAACGTGTGGGACAAAAAAGAAATTAAATAATGTAATCCATACCTTACATTATAATTATTTTTATTATTTTTTTTTATGACAGGAATGGTGCTGACGTGAGAGGGTATTTTGCATGGTCGTTAATGGACAACTTCGAATGGGCCGATGGGTACGGTACGAGGTTTGGGCTTTTGTATGTGGACCGACAGACACTTGAGCGTCGGCCCAAACTGTCGGCCCAGTGGTTTGCTTCCTTTCTCGGCGGACATCCACAAGCACTTGCTAAACCTTCATCCATATTGAATACAAATGCCTTTGATTCCCTCATGTAATATTAATGTAACAACTTTATAAAAAAAATTATTAATATTGAATGGATATTTTTG

mRNA sequence

ATGAAGAACAAGAACAACAAGAAATCAAAAGATCAGATTTTCCCGACCATTTCTTGTTCGGAACTGCCACTTCCTCGGCACTTTATTGTACAGATCGAAGGAGCTTTTCTTGAAGATGGAAGGGGAACGAGTAACTGGGATGCGTTTTCCCACATTCCAGGAGGACGATTTGGTAAAATCAACAGAAGAGGGATCTCTTTCTACAATAAAGTAATTGATCGTTTGTTGCTAAGAGGCATAGAGCCTTTTGTGACCATTCACCATCATGACCTCCCTGATGAGCTGGACAAAAGATATGGAAGGCAAACAAGGCGGATCCATAGGCATAGTGGCATTTGGACACATGCAGAGAAAAAGGTTGTAAAGGGAAGTTTGGACTACATTTATGTGAACCATTACACCACACTTTACACAAAGGACTGCCTTCATTCTATCTGCTCCGACGATGCAAATCGTCCAATTAAAGGCTTCCTCGACACCACCGGCTACCGGGACGGTGTTTCCATTGGTGACCCAACTGGAGTTGACAGATTCTTTGTTGTTCCAAGAGGTTTGGAAAAGATCATTAATTATATCAAAGAAAGGTATCCCGACAACCCCATTTATGTCACTGAAAATGGTTACTCTGCACCGTCTAGTGACGGTAACAACGTTGAAGACATGGTAAATGACACGAAACGAGTCAATTATCACAAGAACTACCTCGCTTCCTTGGCTAAAGCAATGAGGAATGGTGCTGACGTGAGAGGGTATTTTGCATGGTCGTTAATGGACAACTTCGAATGGGCCGATGGGTACGGTACGAGGTTTGGGCTTTTGTATGTGGACCGACAGACACTTGAGCGTCGGCCCAAACTGTCGGCCCAGTGGTTTGCTTCCTTTCTCGGCGGACATCCACAAGCACTTGCTAAACCTTCATCCATATTGAATACAAATGCCTTTGATTCCCTCATGTAATATTAATGTAACAACTTTATAAAAAAAATTATTAATATTGAATGGATATTTTTG

Coding sequence (CDS)

ATGAAGAACAAGAACAACAAGAAATCAAAAGATCAGATTTTCCCGACCATTTCTTGTTCGGAACTGCCACTTCCTCGGCACTTTATTGTACAGATCGAAGGAGCTTTTCTTGAAGATGGAAGGGGAACGAGTAACTGGGATGCGTTTTCCCACATTCCAGGAGGACGATTTGGTAAAATCAACAGAAGAGGGATCTCTTTCTACAATAAAGTAATTGATCGTTTGTTGCTAAGAGGCATAGAGCCTTTTGTGACCATTCACCATCATGACCTCCCTGATGAGCTGGACAAAAGATATGGAAGGCAAACAAGGCGGATCCATAGGCATAGTGGCATTTGGACACATGCAGAGAAAAAGGTTGTAAAGGGAAGTTTGGACTACATTTATGTGAACCATTACACCACACTTTACACAAAGGACTGCCTTCATTCTATCTGCTCCGACGATGCAAATCGTCCAATTAAAGGCTTCCTCGACACCACCGGCTACCGGGACGGTGTTTCCATTGGTGACCCAACTGGAGTTGACAGATTCTTTGTTGTTCCAAGAGGTTTGGAAAAGATCATTAATTATATCAAAGAAAGGTATCCCGACAACCCCATTTATGTCACTGAAAATGGTTACTCTGCACCGTCTAGTGACGGTAACAACGTTGAAGACATGGTAAATGACACGAAACGAGTCAATTATCACAAGAACTACCTCGCTTCCTTGGCTAAAGCAATGAGGAATGGTGCTGACGTGAGAGGGTATTTTGCATGGTCGTTAATGGACAACTTCGAATGGGCCGATGGGTACGGTACGAGGTTTGGGCTTTTGTATGTGGACCGACAGACACTTGAGCGTCGGCCCAAACTGTCGGCCCAGTGGTTTGCTTCCTTTCTCGGCGGACATCCACAAGCACTTGCTAAACCTTCATCCATATTGAATACAAATGCCTTTGATTCCCTCATGTAA

Protein sequence

MKNKNNKKSKDQIFPTISCSELPLPRHFIVQIEGAFLEDGRGTSNWDAFSHIPGGRFGKINRRGISFYNKVIDRLLLRGIEPFVTIHHHDLPDELDKRYGRQTRRIHRHSGIWTHAEKKVVKGSLDYIYVNHYTTLYTKDCLHSICSDDANRPIKGFLDTTGYRDGVSIGDPTGVDRFFVVPRGLEKIINYIKERYPDNPIYVTENGYSAPSSDGNNVEDMVNDTKRVNYHKNYLASLAKAMRNGADVRGYFAWSLMDNFEWADGYGTRFGLLYVDRQTLERRPKLSAQWFASFLGGHPQALAKPSSILNTNAFDSLM
Homology
BLAST of Carg12755-RA vs. NCBI nr
Match: KAG7024855.1 (putative beta-glucosidase 15, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 671.4 bits (1731), Expect = 3.9e-189
Identity = 318/318 (100.00%), Postives = 318/318 (100.00%), Query Frame = 0

Query: 1   MKNKNNKKSKDQIFPTISCSELPLPRHFIVQIEGAFLEDGRGTSNWDAFSHIPGGRFGKI 60
           MKNKNNKKSKDQIFPTISCSELPLPRHFIVQIEGAFLEDGRGTSNWDAFSHIPGGRFGKI
Sbjct: 1   MKNKNNKKSKDQIFPTISCSELPLPRHFIVQIEGAFLEDGRGTSNWDAFSHIPGGRFGKI 60

Query: 61  NRRGISFYNKVIDRLLLRGIEPFVTIHHHDLPDELDKRYGRQTRRIHRHSGIWTHAEKKV 120
           NRRGISFYNKVIDRLLLRGIEPFVTIHHHDLPDELDKRYGRQTRRIHRHSGIWTHAEKKV
Sbjct: 61  NRRGISFYNKVIDRLLLRGIEPFVTIHHHDLPDELDKRYGRQTRRIHRHSGIWTHAEKKV 120

Query: 121 VKGSLDYIYVNHYTTLYTKDCLHSICSDDANRPIKGFLDTTGYRDGVSIGDPTGVDRFFV 180
           VKGSLDYIYVNHYTTLYTKDCLHSICSDDANRPIKGFLDTTGYRDGVSIGDPTGVDRFFV
Sbjct: 121 VKGSLDYIYVNHYTTLYTKDCLHSICSDDANRPIKGFLDTTGYRDGVSIGDPTGVDRFFV 180

Query: 181 VPRGLEKIINYIKERYPDNPIYVTENGYSAPSSDGNNVEDMVNDTKRVNYHKNYLASLAK 240
           VPRGLEKIINYIKERYPDNPIYVTENGYSAPSSDGNNVEDMVNDTKRVNYHKNYLASLAK
Sbjct: 181 VPRGLEKIINYIKERYPDNPIYVTENGYSAPSSDGNNVEDMVNDTKRVNYHKNYLASLAK 240

Query: 241 AMRNGADVRGYFAWSLMDNFEWADGYGTRFGLLYVDRQTLERRPKLSAQWFASFLGGHPQ 300
           AMRNGADVRGYFAWSLMDNFEWADGYGTRFGLLYVDRQTLERRPKLSAQWFASFLGGHPQ
Sbjct: 241 AMRNGADVRGYFAWSLMDNFEWADGYGTRFGLLYVDRQTLERRPKLSAQWFASFLGGHPQ 300

Query: 301 ALAKPSSILNTNAFDSLM 319
           ALAKPSSILNTNAFDSLM
Sbjct: 301 ALAKPSSILNTNAFDSLM 318

BLAST of Carg12755-RA vs. NCBI nr
Match: XP_022935755.1 (beta-glucosidase 18-like [Cucurbita moschata])

HSP 1 Score: 486.9 bits (1252), Expect = 1.3e-133
Identity = 275/489 (56.24%), Postives = 282/489 (57.67%), Query Frame = 0

Query: 25  PRHFI-------VQIEGAFLEDGRGTSNWDAFSHIPG----------------------- 84
           P HF+        QIEGAFLEDGRGTSNWDAFSHIPG                       
Sbjct: 44  PDHFLFGTATSSYQIEGAFLEDGRGTSNWDAFSHIPGKIKNNDTGDVADDHYHRFLEDIE 103

Query: 85  ----------------------GRFGKINRRGISFYNKVIDRLLLRGIEPFVTIHHHDLP 144
                                 GRFGKINRRGISFYNKVIDRLLLRGIEPFVTIHHHDLP
Sbjct: 104 LMHSMGMNAYRFSISWTRILPRGRFGKINRRGISFYNKVIDRLLLRGIEPFVTIHHHDLP 163

Query: 145 DELDKRYG---------------------------------------------------- 204
           DELDKRYG                                                    
Sbjct: 164 DELDKRYGSWMSSQMQEDFVYFARICFEEFGDRVKHWMTINEPNLVVLMAYIKGVYPPAH 223

Query: 205 ----------------------------RQTRRIHR------------------------ 264
                                        +  R++R                        
Sbjct: 224 CSPPFGNCSVGNSDIEPLIVMHNMLLAHAKAVRLYRTHFQGKQGGSIGLVAFGHMYEPFT 283

Query: 265 --------------HSGIWTH-------------------------AEKKVVKGSLDYIY 319
                          +  W +                         AEKKV+KGSLDYIY
Sbjct: 284 DNEFDRQAVDRALIFNFAWVYDPIVYGDYPKEMQTILGSQLPRFSKAEKKVLKGSLDYIY 343

BLAST of Carg12755-RA vs. NCBI nr
Match: XP_022976476.1 (beta-glucosidase 18-like [Cucurbita maxima])

HSP 1 Score: 476.5 bits (1225), Expect = 1.8e-130
Identity = 267/489 (54.60%), Postives = 279/489 (57.06%), Query Frame = 0

Query: 25  PRHFI-------VQIEGAFLEDGRGTSNWDAFSHIPG----------------------- 84
           P HF+        QIEGAFLEDGRGTSNWDAFSHIPG                       
Sbjct: 44  PEHFLFGTATSSYQIEGAFLEDGRGTSNWDAFSHIPGKIKNNDTGDVADDHYHRFLEDIE 103

Query: 85  ----------------------GRFGKINRRGISFYNKVIDRLLLRGIEPFVTIHHHDLP 144
                                 GRFGKINRRGISFYNKVIDRLLLRGIEPFVTIHHHDLP
Sbjct: 104 LMHSMGMNAYRFSISWTRILPRGRFGKINRRGISFYNKVIDRLLLRGIEPFVTIHHHDLP 163

Query: 145 DELDKRYG---------------------------------------------------- 204
           DELDKRYG                                                    
Sbjct: 164 DELDKRYGSWMSSQMQEDFVYFARICFEEFGDRVKHWITINEPNLVVLMAYIKGVYPPAH 223

Query: 205 ----------------------------RQTRRIHR------------------------ 264
                                        +  R++R                        
Sbjct: 224 CSPPFGNCSVGNSDIEPLIVMHNMLLAHAKAVRLYRTHFQGKQGGSIGLVAFGHMYEPFT 283

Query: 265 --------------HSGIWTH-------------------------AEKKVVKGSLDYIY 319
                          +  W +                         AEKK++KGSLDYIY
Sbjct: 284 DNEFDRQAVDRALIFNFAWVYDPIVYGDYPKEMQAILGSQLPRFSKAEKKILKGSLDYIY 343

BLAST of Carg12755-RA vs. NCBI nr
Match: XP_023535085.1 (beta-glucosidase 18-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 474.6 bits (1220), Expect = 6.9e-130
Identity = 268/489 (54.81%), Postives = 278/489 (56.85%), Query Frame = 0

Query: 25  PRHFI-------VQIEGAFLEDGRGTSNWDAFSHIPG----------------------- 84
           P HF+        QIEGAFLEDGRGTSNWDAFSHIPG                       
Sbjct: 44  PDHFLFGTATSSYQIEGAFLEDGRGTSNWDAFSHIPGKIKNNDTGDVADDHYHRFLEDIE 103

Query: 85  ----------------------GRFGKINRRGISFYNKVIDRLLLRGIEPFVTIHHHDLP 144
                                 GRFGKINRRGISFYNKVIDRLLLRGIEPFVTIHHHDLP
Sbjct: 104 LMHSMGMNAYRFSISWTRILPRGRFGKINRRGISFYNKVIDRLLLRGIEPFVTIHHHDLP 163

Query: 145 DELDKRYG---------------------------------------------------- 204
           DELDKRYG                                                    
Sbjct: 164 DELDKRYGSWMSSQMQEDFVYFARICFEEFGDRVKHWMTINEPNLVVLMAYIKGVYPPAH 223

Query: 205 ----------------------------RQTRRIHR------------------------ 264
                                        +  R++R                        
Sbjct: 224 CSPPFGNCSVGNSDIEPLIVMHNMLLAHAKAVRLYRTHFQGKQGGSIGLVAFGHMYEPFT 283

Query: 265 --------------HSGIWTH-------------------------AEKKVVKGSLDYIY 319
                          +  W +                         AEKKV+KGSLDYIY
Sbjct: 284 DNEFDRQAADRALIFNFAWVYDPIVYGDYPKEMQAILGSQLPRFSKAEKKVLKGSLDYIY 343

BLAST of Carg12755-RA vs. NCBI nr
Match: XP_038899221.1 (beta-glucosidase 18-like isoform X1 [Benincasa hispida])

HSP 1 Score: 413.3 bits (1061), Expect = 1.9e-111
Identity = 233/489 (47.65%), Postives = 259/489 (52.97%), Query Frame = 0

Query: 25  PRHFI-------VQIEGAFLEDGRGTSNWDAFSHIPG----------------------- 84
           P HF         QIEG ++EDGRG SNWD FSHIPG                       
Sbjct: 40  PEHFFFGTSTSSYQIEGGYVEDGRGMSNWDVFSHIPGKVKNNDTGDVADDHYHRFMEDIE 99

Query: 85  ----------------------GRFGKINRRGISFYNKVIDRLLLRGIEPFVTIHHHDLP 144
                                 GRFGK+NRRGI+FYNK+ID LLL+GIEPFVTIHHHDLP
Sbjct: 100 LMYSMGMNAYRFSISWTRILPKGRFGKVNRRGITFYNKIIDHLLLKGIEPFVTIHHHDLP 159

Query: 145 DELDKRYG-----------------------------------------RQTRRIH---- 204
           DELDKRYG                                           TR ++    
Sbjct: 160 DELDKRYGSWMSFQMQEDFVYFAKICFKEFGDRVKHWLTINEPNLVTLMAYTRGVYPPAH 219

Query: 205 --------------------RHSGIWTHA------------------------------- 264
                                H+ +  HA                               
Sbjct: 220 CSPPFGNCSVGNSDIEPLIVMHNMLLAHAKAVRLYRTHFQEKQGGLIGIVVFGHMYEPLT 279

Query: 265 -----------------------------------------------EKKVVKGSLDYIY 319
                                                          EK +++GSLDYI 
Sbjct: 280 DHQFDIQAVDRALIFNFAWVYDPIVYGDYPKEMREVFGNQLPSFSDSEKNIIRGSLDYIC 339

BLAST of Carg12755-RA vs. ExPASy Swiss-Prot
Match: Q7XSK0 (Beta-glucosidase 18 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU18 PE=1 SV=2)

HSP 1 Score: 209.1 bits (531), Expect = 7.1e-53
Identity = 132/454 (29.07%), Postives = 180/454 (39.65%), Query Frame = 0

Query: 31  QIEGAFLEDGRGTSNWDAFSHIPG------------------------------------ 90
           QIEGA+LE  +  SNWD F+H+PG                                    
Sbjct: 46  QIEGAYLEGNKSLSNWDVFTHLPGNIKDGSNGDIADDHYHRYEEDVELMNSLGVNAYRFS 105

Query: 91  ---------GRFGKINRRGISFYNKVIDRLLLRGIEPFVTIHHHDLPDELDKRYGRQTRR 150
                    GRFG +N  GI FYNK+ID +LL+GI+PFVT+ H+D+P EL+ RYG     
Sbjct: 106 ISWSRILPKGRFGGVNPAGIDFYNKLIDSILLKGIQPFVTLTHYDIPQELEDRYGAWLNA 165

Query: 151 ------------------------------------------------------------ 210
                                                                       
Sbjct: 166 EIQSDFGHFADVCFGAFGDRVKYWTTFNEPNVAVRHGYMLGTYPPSRCSPPFGHCARGGD 225

Query: 211 ------IHRHSGIWTHA------------------------------------------- 270
                 +  H+ I +HA                                           
Sbjct: 226 SHAEPYVAAHNVILSHATAIEIYKRKYQSKQRGMIGMVLYSTWYEPLRDVPEDRLATERA 285

Query: 271 -----------------------------------EKKVVKGSLDYIYVNHYTTLYTKDC 296
                                              +++ ++  LD+I VNHYTTLY +DC
Sbjct: 286 LAFETPWFLDPLVYGDYPPEMRQILGGRLPSFSPEDRRKLRYKLDFIGVNHYTTLYARDC 345

BLAST of Carg12755-RA vs. ExPASy Swiss-Prot
Match: Q7XPY7 (Probable inactive beta-glucosidase 14 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU14 PE=2 SV=2)

HSP 1 Score: 201.1 bits (510), Expect = 1.9e-50
Identity = 138/457 (30.20%), Postives = 179/457 (39.17%), Query Frame = 0

Query: 31  QIEGAFLEDGRGTSNWDAFSH--------------------------------------- 90
           Q+EG +LE  +G SNWD F+H                                       
Sbjct: 43  QVEGGYLEGNKGLSNWDVFTHKQGTIEDGSNGDTANDHYHRYMEDIELMHSLGVNSYRFS 102

Query: 91  ------IPGGRFGKINRRGISFYNKVIDRLLLRGIEPFVTIHHHDLPDELDKRYGRQTR- 150
                 +P GRFG +N  G++FYN +ID L+ +GI+PFVTI H+D+P ELD+RYG     
Sbjct: 103 ISWARILPKGRFGDVNPDGVAFYNALIDGLVQKGIQPFVTICHYDIPHELDERYGGWLSP 162

Query: 151 ------------------------------------------------------------ 210
                                                                       
Sbjct: 163 EIQKDFSYFAEVCFKLFGDRIKFWTTFNQPNLSIKFSYMDGFYSPGRCSEPFGKCALGNS 222

Query: 211 ----RIHRHSGIWTHA-------------------------------------------- 270
                +  H+ I +HA                                            
Sbjct: 223 SIEPYVAGHNIILSHANAVSVYRNKYQGKQGGQIGIALSITWYEPFRNTTIDLLAVKRAL 282

Query: 271 ------------------EKKVVKG-----------------SLDYIYVNHYTTLYTKDC 298
                             E + V G                  LD+I +NHYTT Y KDC
Sbjct: 283 SFGASWFLDPILLGDYPTEMREVLGQSLPKFTSKQKNRLQSTKLDFIGLNHYTTCYVKDC 342

BLAST of Carg12755-RA vs. ExPASy Swiss-Prot
Match: Q7XSK2 (Beta-glucosidase 16 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU16 PE=1 SV=2)

HSP 1 Score: 196.4 bits (498), Expect = 4.8e-49
Identity = 129/455 (28.35%), Postives = 184/455 (40.44%), Query Frame = 0

Query: 31  QIEGAFLEDGRGTSNWDAFSH--------------------------------------- 90
           QIEGA+L+D +G +NWD F+H                                       
Sbjct: 47  QIEGAYLDDNKGLNNWDVFTHTQAGRISDGRNGDVADDHYHRYTEDVDILHNLGVNSYRF 106

Query: 91  -------IPGGRFGKINRRGISFYNKVIDRLLLRGIEPFVTIHHHDLPDELDKRYG---- 150
                  +P GR G +N  GI+FYN++I+ LL +GI+PFVT++H D+P EL+ RYG    
Sbjct: 107 SISWARILPRGRLGGVNSAGIAFYNRLINALLQKGIQPFVTLNHFDIPHELETRYGGWLG 166

Query: 151 ------------------------------------------------------------ 210
                                                                       
Sbjct: 167 AAIREEFEYYSDVCFNAFGDRVRFWTTFNEPNLSTRHQYILGEFPPNHCSPPFGNCSSGD 226

Query: 211 ------------------------------------------------------RQTRRI 270
                                                                 R  RR 
Sbjct: 227 SRREPYAAAHNILLSHAAAVHNYKTNYQAKQGGSIGIVIAVKWYEPLTNSTEDVRAARRA 286

Query: 271 HRHSGIW-------------------------THAEKKVVKGS-LDYIYVNHYTTLYTKD 296
                 W                         T  EKK+++ + +D+I +NHYT +Y KD
Sbjct: 287 LAFEVDWFLDPIFFGDYPREMREILSSNLPKFTPEEKKLLQNNKVDFIGINHYTAIYAKD 346

BLAST of Carg12755-RA vs. ExPASy Swiss-Prot
Match: Q7XPY5 (Putative beta-glucosidase 15 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU15 PE=5 SV=2)

HSP 1 Score: 189.5 bits (480), Expect = 5.8e-47
Identity = 96/227 (42.29%), Postives = 137/227 (60.35%), Query Frame = 0

Query: 71  VIDRLLLRGIEPFVTIHHHDLPDELDKRYGRQTRRIHRHSGIWTHAEKKVVKGSLDYIYV 130
           + D+L LR ++P   I   + P E+ +       +       +T  EKK+++  +D+I +
Sbjct: 131 ITDKLSLRFLDP---IFFGEYPREMREILSSNLPK-------FTPEEKKLLQNKVDFIGI 190

Query: 131 NHYTTLYTKDCLHSICSDDANRPIKGFLDTTGYRDGVSIGDPTGVDRFFVVPRGLEKIIN 190
           N YT +Y KDC++S C+ +        + TTG R+G  IG PT    +FVVP  +E  + 
Sbjct: 191 NQYTAIYAKDCIYSPCALNTYEG-NALVYTTGVRNGAKIGKPTAFSTYFVVPESIESAVM 250

Query: 191 YIKERYPDNPIYVTENGYSAPSSDGNNVEDMVNDTKRVNYHKNYLASLAKAMRNGADVRG 250
           Y+  RY D  IY+TENGYS  S    N+ED++ND +RVNY + YL  L+ A+R GA+V G
Sbjct: 251 YVNGRYKDTTIYITENGYSQHSD--TNMEDLINDVERVNYLQGYLKYLSSAVRKGANVGG 310

Query: 251 YFAWSLMDNFEWADGYGTRFGLLYVDRQTLERRPKLSAQWFASFLGG 298
           YF WSL+DNFEW  GY  +FGL +VD  T ER PK+SA+W+  FL G
Sbjct: 311 YFMWSLIDNFEWVFGYTIKFGLYHVDFDTQERIPKMSAKWYRDFLTG 344

BLAST of Carg12755-RA vs. ExPASy Swiss-Prot
Match: O80689 (Beta-glucosidase 45 OS=Arabidopsis thaliana OX=3702 GN=BGLU45 PE=1 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 3.5e-44
Identity = 126/474 (26.58%), Postives = 184/474 (38.82%), Query Frame = 0

Query: 23  PLPRHFI-------VQIEGAFLEDGRGTSNWDAFSH------------------------ 82
           P P  F+        Q EGAFL DG+  +NWD F+H                        
Sbjct: 37  PFPSDFLFGTASSAYQYEGAFLTDGKSLNNWDVFTHKNPGKILDKNNADRAVDQYNRFLE 96

Query: 83  ----------------------IPGGRFGKINRRGISFYNKVIDRLLLRGIEPFVTIHHH 142
                                 +P GRFG+IN  GI +YN  ID L+ RGI+PFVT++H 
Sbjct: 97  DIQLMSFLGVNSYRFSISWCRILPRGRFGEINYLGIKYYNIFIDALISRGIKPFVTLNHV 156

Query: 143 DLPDELD-----------------------KRYGRQTRR--------------------- 202
           D P EL+                       K +G + +                      
Sbjct: 157 DYPQELEDRFQSWLNPEMQKEFGYLADICFKHFGNRVKYWTTLNEPNQQLILGYLTGKFP 216

Query: 203 ---------------------IHRHSGIWTHAE---------KKVVKGSL---------- 262
                                I  H+ I  HA+         +K  KGS+          
Sbjct: 217 PSRCSSPYGNCSQGNSETEPFIAAHNMILAHAKAVNIYKTKYQKEQKGSIGIVVQTSWFE 276

Query: 263 ------------------------------------------------------------ 299
                                                                       
Sbjct: 277 PISDSNADKEAAERAQSFYSNWILDPVIYGKYPKEMVDILGPALPQFSSNEVKNLEKSRA 336

BLAST of Carg12755-RA vs. ExPASy TrEMBL
Match: A0A6J1FBJ3 (beta-glucosidase 18-like OS=Cucurbita moschata OX=3662 GN=LOC111442578 PE=3 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 6.5e-134
Identity = 275/489 (56.24%), Postives = 282/489 (57.67%), Query Frame = 0

Query: 25  PRHFI-------VQIEGAFLEDGRGTSNWDAFSHIPG----------------------- 84
           P HF+        QIEGAFLEDGRGTSNWDAFSHIPG                       
Sbjct: 44  PDHFLFGTATSSYQIEGAFLEDGRGTSNWDAFSHIPGKIKNNDTGDVADDHYHRFLEDIE 103

Query: 85  ----------------------GRFGKINRRGISFYNKVIDRLLLRGIEPFVTIHHHDLP 144
                                 GRFGKINRRGISFYNKVIDRLLLRGIEPFVTIHHHDLP
Sbjct: 104 LMHSMGMNAYRFSISWTRILPRGRFGKINRRGISFYNKVIDRLLLRGIEPFVTIHHHDLP 163

Query: 145 DELDKRYG---------------------------------------------------- 204
           DELDKRYG                                                    
Sbjct: 164 DELDKRYGSWMSSQMQEDFVYFARICFEEFGDRVKHWMTINEPNLVVLMAYIKGVYPPAH 223

Query: 205 ----------------------------RQTRRIHR------------------------ 264
                                        +  R++R                        
Sbjct: 224 CSPPFGNCSVGNSDIEPLIVMHNMLLAHAKAVRLYRTHFQGKQGGSIGLVAFGHMYEPFT 283

Query: 265 --------------HSGIWTH-------------------------AEKKVVKGSLDYIY 319
                          +  W +                         AEKKV+KGSLDYIY
Sbjct: 284 DNEFDRQAVDRALIFNFAWVYDPIVYGDYPKEMQTILGSQLPRFSKAEKKVLKGSLDYIY 343

BLAST of Carg12755-RA vs. ExPASy TrEMBL
Match: A0A6J1IH03 (beta-glucosidase 18-like OS=Cucurbita maxima OX=3661 GN=LOC111476863 PE=3 SV=1)

HSP 1 Score: 476.5 bits (1225), Expect = 8.8e-131
Identity = 267/489 (54.60%), Postives = 279/489 (57.06%), Query Frame = 0

Query: 25  PRHFI-------VQIEGAFLEDGRGTSNWDAFSHIPG----------------------- 84
           P HF+        QIEGAFLEDGRGTSNWDAFSHIPG                       
Sbjct: 44  PEHFLFGTATSSYQIEGAFLEDGRGTSNWDAFSHIPGKIKNNDTGDVADDHYHRFLEDIE 103

Query: 85  ----------------------GRFGKINRRGISFYNKVIDRLLLRGIEPFVTIHHHDLP 144
                                 GRFGKINRRGISFYNKVIDRLLLRGIEPFVTIHHHDLP
Sbjct: 104 LMHSMGMNAYRFSISWTRILPRGRFGKINRRGISFYNKVIDRLLLRGIEPFVTIHHHDLP 163

Query: 145 DELDKRYG---------------------------------------------------- 204
           DELDKRYG                                                    
Sbjct: 164 DELDKRYGSWMSSQMQEDFVYFARICFEEFGDRVKHWITINEPNLVVLMAYIKGVYPPAH 223

Query: 205 ----------------------------RQTRRIHR------------------------ 264
                                        +  R++R                        
Sbjct: 224 CSPPFGNCSVGNSDIEPLIVMHNMLLAHAKAVRLYRTHFQGKQGGSIGLVAFGHMYEPFT 283

Query: 265 --------------HSGIWTH-------------------------AEKKVVKGSLDYIY 319
                          +  W +                         AEKK++KGSLDYIY
Sbjct: 284 DNEFDRQAVDRALIFNFAWVYDPIVYGDYPKEMQAILGSQLPRFSKAEKKILKGSLDYIY 343

BLAST of Carg12755-RA vs. ExPASy TrEMBL
Match: A0A1S3BVA7 (beta-glucosidase 18-like OS=Cucumis melo OX=3656 GN=LOC103493887 PE=3 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 3.8e-110
Identity = 235/500 (47.00%), Postives = 261/500 (52.20%), Query Frame = 0

Query: 7   KKSKDQIFPTISCSELPLPRHFIVQIEGAFLEDGRGTSNWDAFSHIPG------------ 66
           KKSKDQIF TIS  ELP P     +++G F+EDGRG SNWD FSHIPG            
Sbjct: 45  KKSKDQIFQTISSLELPHPP---TRLKGGFVEDGRGKSNWDVFSHIPGKIKDNDTGDVAD 104

Query: 67  ---------------------------------GRFGKINRRGISFYNKVIDRLLLRGIE 126
                                            GRFG++NRRGI FYNK+ID LLL+GIE
Sbjct: 105 DHYHRFTEDIELMSSMGMNAYRFSISWTRILPKGRFGEVNRRGIIFYNKIIDNLLLKGIE 164

Query: 127 PFVTIHHHDLPDELDKRYGRQTRR------------------------------------ 186
           PFVTIHHHDLPDELDKRYG                                         
Sbjct: 165 PFVTIHHHDLPDELDKRYGSWMSSQMQEDFVYFARICFEEFGDRVKHWITINEPNLVTLM 224

Query: 187 -----------------------------IHRHSGIWTHA-------------------- 246
                                        I  H+ +  HA                    
Sbjct: 225 AYIRGVYPPAHCSPPFGNCSAGNSDIEPLIVMHNMLLAHAKAVFLYRTHFQKKQGGSIGI 284

Query: 247 ----------------------------------------------------------EK 306
                                                                     EK
Sbjct: 285 TAVGHMYEPLTNHEFDIEAVDRALIFNFAWVYDPIVYGDYPKEMREVFGSQLPSFSKTEK 344

Query: 307 KVVKGSLDYIYVNHYTTLYTKDCLHSICSDDANRPIKGFLDTTGYRDGVSIGDPTGVDRF 318
            ++KGSLDYI VNHYTTLYTKDCLHS CS+  +RPIKGFLDTTGYRD VSIGDPTGVDRF
Sbjct: 345 SIIKGSLDYICVNHYTTLYTKDCLHSPCSNGGDRPIKGFLDTTGYRDSVSIGDPTGVDRF 404

BLAST of Carg12755-RA vs. ExPASy TrEMBL
Match: A0A1S3BZ78 (beta-glucosidase 18-like OS=Cucumis melo OX=3656 GN=LOC103495056 PE=3 SV=1)

HSP 1 Score: 387.5 bits (994), Expect = 5.4e-104
Identity = 215/475 (45.26%), Postives = 251/475 (52.84%), Query Frame = 0

Query: 32  IEGAFLEDGRGTSNWDAFSHIPG------------------------------------- 91
           IEGA+LEDG+G SNWD FSH+PG                                     
Sbjct: 22  IEGAYLEDGKGLSNWDVFSHLPGRIRNNDTGDVADDHYHRFLEDIELMHSMGMNSYRFSI 81

Query: 92  --------GRFGKINRRGISFYNKVIDRLLLRGIEPFVTIHHHDLPDELDKRYGR----- 151
                   GRFGK+NRRGI+FYNK+ID LLL+GIEPFVTIHHHDLP ELDKRYG      
Sbjct: 82  SWTRILPKGRFGKVNRRGIAFYNKIIDNLLLKGIEPFVTIHHHDLPAELDKRYGSWMSAE 141

Query: 152 ------------------------------------QTRRIH------------------ 211
                                                T+ ++                  
Sbjct: 142 MQEDFVYFAKICFEEFGDRVKYWITINEPNVITLMGYTKGVYPPGHCSPPFGNCSIGNAD 201

Query: 212 ------RHSGIWTHA--------------------------------------------- 271
                  H+ +  HA                                             
Sbjct: 202 IEPLIVMHNMLLAHAKAVRLYCTHFQKKQGGSIGIVAYGHMYEPLTDHEYDIKAAERAFI 261

Query: 272 ---------------------------------EKKVVKGSLDYIYVNHYTTLYTKDCLH 319
                                            EK +++GSLD++ VNHYTT YTKDCLH
Sbjct: 262 FIFAWVYDPIVYGDYPKEMQEILGSQLPSFSDIEKNIIRGSLDFVCVNHYTTFYTKDCLH 321

BLAST of Carg12755-RA vs. ExPASy TrEMBL
Match: A0A0A0L4S1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G638400 PE=3 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 2.7e-103
Identity = 221/489 (45.19%), Postives = 245/489 (50.10%), Query Frame = 0

Query: 25  PRHFI-------VQIEGAFLEDGRGTSNWDAFSHIPG----------------------- 84
           P HF         QIEG ++EDGRGTSNWD FSHIPG                       
Sbjct: 36  PNHFFFGTSTSSYQIEGGYVEDGRGTSNWDVFSHIPGNIKNSDTGDVADDHYHRFMEDIE 95

Query: 85  ----------------------GRFGKINRRGISFYNKVIDRLLLRGIEPFVTIHHHDLP 144
                                 GRFGK+NRRGI FYNK+ID LLL+GIEPFVTIHHHDLP
Sbjct: 96  MMSSMGMNAYRFSISWTRILPKGRFGKVNRRGIVFYNKIIDNLLLKGIEPFVTIHHHDLP 155

Query: 145 DELDKRYGRQTRR----------------------------------------------- 204
           DELDKRYG                                                    
Sbjct: 156 DELDKRYGSWMSSHMQEDFVYFAKICFKEFGDRVKHWITINEPNLVTLMGYIKGVYPPAH 215

Query: 205 ------------------IHRHSGIWTHA------------------------------- 264
                             I  H+ +  HA                               
Sbjct: 216 CSPPFGNCSVGNSDIEPLIVMHNMLLAHAKAVFIYRTQFQKKQGGSIGLVAYCHMYEPLT 275

Query: 265 -----------------------------------------------EKKVVKGSLDYIY 319
                                                          EK ++KGSLDYI 
Sbjct: 276 NNEFDLQAVDRALIFSFAWVYDPIVYGDYPKEMREVFGSQLPSFSNTEKNIIKGSLDYIC 335

BLAST of Carg12755-RA vs. TAIR 10
Match: AT1G61820.1 (beta glucosidase 46 )

HSP 1 Score: 184.1 bits (466), Expect = 1.7e-46
Identity = 131/471 (27.81%), Postives = 182/471 (38.64%), Query Frame = 0

Query: 23  PLPRHFI-------VQIEGAFLEDGRGTSNWDAFSH------------------------ 82
           P P  F+        Q EGAFL DG+G +NWD F+H                        
Sbjct: 34  PFPSDFLFGTASSAFQYEGAFLTDGKGLNNWDVFAHENPGKIVDGSNGDIATDQYHRYME 93

Query: 83  ----------------------IPGGRFGKINRRGISFYNKVIDRLLLRGIEPFVTIHHH 142
                                 +P GRFG IN +GI +YN +ID L+ +GI PFVT++H 
Sbjct: 94  DIQSMNFLGVNSYRLSISWSRVLPNGRFGVINYKGIKYYNNLIDALIKKGITPFVTLNHF 153

Query: 143 DLPDELDKR--------------------------------------------------- 202
           D P EL+ R                                                   
Sbjct: 154 DYPQELENRFKSWLSSEMQKDFGYLADICFKHFGDRVKHWITINEPNQHISLAYRSGLFP 213

Query: 203 -------YGRQTRR-------IHRHSGIWTHA------------EKKVVKGSL------- 262
                  YG  T         I  H+ I  HA            E+K + G +       
Sbjct: 214 PARCSMPYGNCTHGNSETEPFIAAHNMILAHAKAIQIYRTKYQREQKGIIGIVVQTSWFE 273

Query: 263 ------------------------------------------------------------ 296
                                                                       
Sbjct: 274 PISDSIADKNAAERAQSFYSNWILDPVVYGKYPEEMVNLLGSALPKFSSNEMNSLMSYKS 333

BLAST of Carg12755-RA vs. TAIR 10
Match: AT1G61810.1 (beta-glucosidase 45 )

HSP 1 Score: 180.3 bits (456), Expect = 2.5e-45
Identity = 126/474 (26.58%), Postives = 184/474 (38.82%), Query Frame = 0

Query: 23  PLPRHFI-------VQIEGAFLEDGRGTSNWDAFSH------------------------ 82
           P P  F+        Q EGAFL DG+  +NWD F+H                        
Sbjct: 37  PFPSDFLFGTASSAYQYEGAFLTDGKSLNNWDVFTHKNPGKILDKNNADRAVDQYNRFLE 96

Query: 83  ----------------------IPGGRFGKINRRGISFYNKVIDRLLLRGIEPFVTIHHH 142
                                 +P GRFG+IN  GI +YN  ID L+ RGI+PFVT++H 
Sbjct: 97  DIQLMSFLGVNSYRFSISWCRILPRGRFGEINYLGIKYYNIFIDALISRGIKPFVTLNHV 156

Query: 143 DLPDELD-----------------------KRYGRQTRR--------------------- 202
           D P EL+                       K +G + +                      
Sbjct: 157 DYPQELEDRFQSWLNPEMQKEFGYLADICFKHFGNRVKYWTTLNEPNQQLILGYLTGKFP 216

Query: 203 ---------------------IHRHSGIWTHAE---------KKVVKGSL---------- 262
                                I  H+ I  HA+         +K  KGS+          
Sbjct: 217 PSRCSSPYGNCSQGNSETEPFIAAHNMILAHAKAVNIYKTKYQKEQKGSIGIVVQTSWFE 276

Query: 263 ------------------------------------------------------------ 299
                                                                       
Sbjct: 277 PISDSNADKEAAERAQSFYSNWILDPVIYGKYPKEMVDILGPALPQFSSNEVKNLEKSRA 336

BLAST of Carg12755-RA vs. TAIR 10
Match: AT4G21760.1 (beta-glucosidase 47 )

HSP 1 Score: 179.9 bits (455), Expect = 3.3e-45
Identity = 132/463 (28.51%), Postives = 179/463 (38.66%), Query Frame = 0

Query: 31  QIEGAFLEDGRGTSNWDAFSHI-------------------------------------- 90
           Q EGA+L DG+  SNWD F++I                                      
Sbjct: 73  QYEGAYLTDGKTLSNWDVFTNISGKIADGSHGKVAVDHYHRYPGDLDLMEDLGVNSYRLS 132

Query: 91  -------PGGRFGKINRRGISFYNKVIDRLLLRGIEPFVTIHHHDLPDELDKRYGR---- 150
                  P GRFG +N  GI  YN++I+ +L  GIEPFVT+ H+D+P EL+ RYG     
Sbjct: 133 LSWARILPKGRFGDVNMGGIDHYNRMINDILKTGIEPFVTLTHYDIPQELEYRYGSWLNP 192

Query: 151 QTRR--------IHRHSG------------------------------------------ 210
           Q R           RH G                                          
Sbjct: 193 QIREDFEHYANICFRHFGDRVKFWSTFNEPNVQVILGYRTGTYPPSRCSKPFGNCSCGDS 252

Query: 211 ----------------------------------------IW------------------ 270
                                                   IW                  
Sbjct: 253 YIEPLVAAHNIILSHLAAVNLYRTKFQEQQRGQIGIVMNTIWFEPISDSLADRLAADRAQ 312

Query: 271 -------------------------------THAEKKVVKGSLDYIYVNHYTTLYTKDCL 305
                                          T  + K  K +LD+I +N YT+ Y KDCL
Sbjct: 313 AFYLTWFLDPVVFGRYPREMREILGDDLPEFTKDDLKSSKNALDFIGINQYTSRYAKDCL 372

BLAST of Carg12755-RA vs. TAIR 10
Match: AT1G61820.3 (beta glucosidase 46 )

HSP 1 Score: 165.6 bits (418), Expect = 6.4e-41
Identity = 77/171 (45.03%), Postives = 111/171 (64.91%), Query Frame = 0

Query: 126 DYIYVNHYTTLYTKDCLHSIC-SDDANRPIKGFLDTTGYRDGVSIGDPTGVDRFFVVPRG 185
           D++ +NHYT+ + +DCL + C S D     +G       +  VSIG+ T V+   + P G
Sbjct: 195 DFLGINHYTSYFIQDCLITACNSGDGASKSEGLALKLDRKGNVSIGELTDVNWQHIDPNG 254

Query: 186 LEKIINYIKERYPDNPIYVTENGYSAPSSDGNNVEDMVNDTKRVNYHKNYLASLAKAMRN 245
             K++NY+K RY + P+Y+TENG+         VE++++DTKR+ Y   YL +L  AMR+
Sbjct: 255 FRKMLNYLKNRYHNIPMYITENGFGQLQKPETTVEELLHDTKRIQYLSGYLDALKAAMRD 314

Query: 246 GADVRGYFAWSLMDNFEWADGYGTRFGLLYVDRQTLERRPKLSAQWFASFL 296
           GA+V+GYFAWSL+DNFEW  GY  RFGL +VD  TL+R PK SA W+ +F+
Sbjct: 315 GANVKGYFAWSLLDNFEWLYGYKVRFGLFHVDFTTLKRTPKQSATWYKNFI 365

BLAST of Carg12755-RA vs. TAIR 10
Match: AT1G61810.3 (beta-glucosidase 45 )

HSP 1 Score: 164.1 bits (414), Expect = 1.9e-40
Identity = 120/458 (26.20%), Postives = 174/458 (37.99%), Query Frame = 0

Query: 23  PLPRHFI-------VQIEGAFLEDGRGTSNWDAFSH------------------------ 82
           P P  F+        Q EGAFL DG+  +NWD F+H                        
Sbjct: 37  PFPSDFLFGTASSAYQYEGAFLTDGKSLNNWDVFTHKNPGKILDKNNADRAVDQYNRFLE 96

Query: 83  ----------------------IPGGRFGKINRRGISFYNKVIDRLLLRGIEPFVTIHHH 142
                                 +P GRFG+IN  GI +YN  ID L+ RGI+PFVT++H 
Sbjct: 97  DIQLMSFLGVNSYRFSISWCRILPRGRFGEINYLGIKYYNIFIDALISRGIKPFVTLNHV 156

Query: 143 DLPDELD-----------------------KRYGRQTRR--------------------- 202
           D P EL+                       K +G + +                      
Sbjct: 157 DYPQELEDRFQSWLNPEMQKEFGYLADICFKHFGNRVKYWTTLNEPNQQLILGYLTGKFP 216

Query: 203 ---------------------IHRHSGIWTHAE---------KKVVKGSL---------- 262
                                I  H+ I  HA+         +K  KGS+          
Sbjct: 217 PSRCSSPYGNCSQGNSETEPFIAAHNMILAHAKAVNIYKTKYQKEQKGSIGIVVQTSWFE 276

Query: 263 ------------------------------------------------------------ 283
                                                                       
Sbjct: 277 PISDSNADKEAAERAQSFYSNWILDPVIYGKYPKEMVDILGPALPQFSSNEVKNLEKSRA 336

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7024855.13.9e-189100.00putative beta-glucosidase 15, partial [Cucurbita argyrosperma subsp. argyrosperm... [more]
XP_022935755.11.3e-13356.24beta-glucosidase 18-like [Cucurbita moschata][more]
XP_022976476.11.8e-13054.60beta-glucosidase 18-like [Cucurbita maxima][more]
XP_023535085.16.9e-13054.81beta-glucosidase 18-like [Cucurbita pepo subsp. pepo][more]
XP_038899221.11.9e-11147.65beta-glucosidase 18-like isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q7XSK07.1e-5329.07Beta-glucosidase 18 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU18 PE=1 SV=2[more]
Q7XPY71.9e-5030.20Probable inactive beta-glucosidase 14 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q7XSK24.8e-4928.35Beta-glucosidase 16 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU16 PE=1 SV=2[more]
Q7XPY55.8e-4742.29Putative beta-glucosidase 15 OS=Oryza sativa subsp. japonica OX=39947 GN=BGLU15 ... [more]
O806893.5e-4426.58Beta-glucosidase 45 OS=Arabidopsis thaliana OX=3702 GN=BGLU45 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FBJ36.5e-13456.24beta-glucosidase 18-like OS=Cucurbita moschata OX=3662 GN=LOC111442578 PE=3 SV=1[more]
A0A6J1IH038.8e-13154.60beta-glucosidase 18-like OS=Cucurbita maxima OX=3661 GN=LOC111476863 PE=3 SV=1[more]
A0A1S3BVA73.8e-11047.00beta-glucosidase 18-like OS=Cucumis melo OX=3656 GN=LOC103493887 PE=3 SV=1[more]
A0A1S3BZ785.4e-10445.26beta-glucosidase 18-like OS=Cucumis melo OX=3656 GN=LOC103495056 PE=3 SV=1[more]
A0A0A0L4S12.7e-10345.19Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G638400 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G61820.11.7e-4627.81beta glucosidase 46 [more]
AT1G61810.12.5e-4526.58beta-glucosidase 45 [more]
AT4G21760.13.3e-4528.51beta-glucosidase 47 [more]
AT1G61820.36.4e-4145.03beta glucosidase 46 [more]
AT1G61810.31.9e-4026.20beta-glucosidase 45 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001360Glycoside hydrolase family 1PRINTSPR00131GLHYDRLASE1coord: 269..281
score: 49.85
coord: 124..138
score: 32.62
coord: 224..235
score: 35.67
coord: 245..262
score: 63.78
coord: 201..209
score: 56.74
IPR001360Glycoside hydrolase family 1PFAMPF00232Glyco_hydro_1coord: 113..294
e-value: 2.5E-43
score: 148.2
coord: 52..105
e-value: 1.7E-12
score: 46.5
IPR001360Glycoside hydrolase family 1PANTHERPTHR10353GLYCOSYL HYDROLASEcoord: 51..100
coord: 114..302
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 16..105
e-value: 3.8E-18
score: 67.2
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 106..296
e-value: 3.9E-63
score: 215.5
NoneNo IPR availablePANTHERPTHR10353:SF175BETA-GLUCOSIDASE 18-LIKEcoord: 114..302
NoneNo IPR availablePANTHERPTHR10353:SF175BETA-GLUCOSIDASE 18-LIKEcoord: 51..100
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 30..295

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg12755Carg12755gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg12755-RA:9Carg12755-RA:9exon
Carg12755-RA:8Carg12755-RA:8exon
Carg12755-RA:7Carg12755-RA:7exon
Carg12755-RA:6Carg12755-RA:6exon
Carg12755-RA:5Carg12755-RA:5exon
Carg12755-RA:4Carg12755-RA:4exon
Carg12755-RA:3Carg12755-RA:3exon
Carg12755-RA:2Carg12755-RA:2exon
Carg12755-RA:1Carg12755-RA:1exon


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg12755-RA:three_prime_utrCarg12755-RA:three_prime_utrthree_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg12755-RA:cdsCarg12755-RA:cds_9CDS
Carg12755-RA:cdsCarg12755-RA:cds_8CDS
Carg12755-RA:cdsCarg12755-RA:cds_7CDS
Carg12755-RA:cdsCarg12755-RA:cds_6CDS
Carg12755-RA:cdsCarg12755-RA:cds_5CDS
Carg12755-RA:cdsCarg12755-RA:cds_4CDS
Carg12755-RA:cdsCarg12755-RA:cds_3CDS
Carg12755-RA:cdsCarg12755-RA:cds_2CDS
Carg12755-RA:cdsCarg12755-RA:cdsCDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg12755-RACarg12755-RA-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds