Carg04638-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg04638-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Glucan endo-1,3-beta-D-glucosidase
Location	Carg_Chr11: 12818108 .. 12820291 (+)
Sequence length	1389
RNA-Seq Expression	Carg04638-RA
Synteny	Carg04638-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGCGCCTAACCCTCTCTCTTCTTCTTCGTCTTCCTCCTCCTCCTCTCCGCGAGCTTTCCTGTTCTTACAGAGGCTGGTTCCATTGGTGTCAACTATGGCAGAATCGCCAACAATCTCCCCTCCGCCCTCAAGGTCGTTAACCTCCTCAAATCCCGCGGCCTTCAAAGGGTTAAAGTTTACGACACCGATCCGGCTGTTCTCAGAGCCATTTCCGGCTCTGGCATTAAAGTCACAGTCGATTTGCCCAACGAACTCCTCTTCGCCGCCGCCAAACGCCTCACCTTTGCCTACGCATGGGTCGAAAAGAACATCGTCGCCTACTACCCTTCCACGGAAATCGAAGCCATTGCTGTCGGCAACGAAGTCTTTGTCGACCCACATAACACGACGTCGTTTCTCATTCCGGCCATGAAGAACATTCACCAAGCCCTCGTTAAATACAACCTCCATTCCTCCATTAAGGTCTCTTCTCCTATAGCCTTAAGCGCCCTGCAAAACTCTTACCCTTCCTCCGCCGGTTCATTCCGTCCGGAGCTTATCGAATCGGTTTTCCGGCCAATGCTGGAGTTTCTCCGCCAGACTGGCTCCTATTTGATGGTTAACGCTTACCCATTTTTCGCTTACGAGTCTAACTCCGATGTCATTTCTTTAGACTACGCTCTTTTCCGGGAGAACCCCGGCGTGGTGGATGCCGGTAGCGGTTACCGGTACTTCAGTTTATTCGACGCCCAAATCGACGCCGTTTTCGCCGCCATGTCGGCTCTGAAATACGACGACATTAAAATGGTGGTTACCGAAACTGGGTGGCCGTCCAAAGGCGACGAGAACGAGATTGGCGCTAGTGTCGAAAACGCCGCCGCATACAACGGGAATCTGGTCCGGCGGATTTTGAGCGGCGGCGGAACTCCATTGCGGCCGAAAGCAGATCTGACCGTCTATTTATTCGCCCTTTTCAACGAAAACAAAAAGAACGGTCCCACATCGGAAAGAAACTACGGCTTATTTTACCCTAACGAAGAATCAGTTTACGATATCCCTTTCACAACAGAGGGGTTGAAAGACTACCACGACAAACCATCGCCGTCGCCGTCGCCGGCTACCGGTGGCGACGGGAAGTCCGGACCAGTCCACGGCGATGGGAATGTTTCAAAAAGTCAAACGGGGAATACGTGGTGCGTTGCGAGTGGTGAAGCGGGGAAAGAGAAGCTGCAGGCGAGTCTAGATTATGCTTGTGGTGAAGGAGGGGCGGATTGCCGTCCAATCCAAGTGGGTGCCACGTGTTACAATCCTAACACGCTTGAGGCACATGCTTCGTATGCTTTCAATAGTTATTATCAGAAGAACGGGCGAAAGACCGGCACGTGTTACTTTGGAGGGGCGGCTTACGTAGTCACGCAACCACCGAGTAAGTACTCTATAGTTTTCTCTTTTTTTATTGTTTTATTTTTCTACGACTCGAGCCGACCGGGTCCGGCCTGGTTTTCATTTTTTAATGCGCTGACGTGGTCCACGAGGTGGACTGATGGAGGTGCAGGCCAGCTCAGTAAGAAGCCAAATTTCACAGCCCACATGCCGAGCATTATTAGAGAAAGGGAATCTTAGTTTTGCTTACGTGGACCCATCTTTTTGGGAACCCCTAATTAATCTTTTTATTCAAATATTTATTTTACCTATTTTAAATTTTAAAATTTGGTTTCTAAAAGCTGATAAAAATTCAATTTGGTCTCTTCTTTGGGTTTTAAATTAAGAGAAAAAAATGGGTTTTTTTTTGTGTCTATTCGGTTCGCGGGTTTGGATGTGAACCCTCGTTGAGGTCGTTCGAAACAGCAACTAACTAAAAATTCTATTCGGTTCATGGGTTTTGGACGTGAACCCTAATTTAGGTCATTTGGATCGGCAACTAACCATAAATTATATTCGGTTCGTAAGTTTGGATGTGAACCCTGTTGAGATCGTTTAGGTCACTAACTCAATCAGTTTGAAATTTCGAGCGGGTCTAAACAAATTTCTCAGTTTTTTTTTTTTTTTAATGTGTTTTTAATTATGGGTGTATTTTTGTGTGTGCAGAGTATGGGAGCTGTGAATTCCCGACAGGGTATTGAAGTTGAAGGGACAACGGAGAGAAAAAAAAAGGTTAATTTTTATGTTTGGTTTTAGAGTATGAGAGAATTCTTTTGTAA

mRNA sequence

ATGGAGCGCCTAACCCTCTCTCTTCTTCTTCAGGCTGGTTCCATTGGTGTCAACTATGGCAGAATCGCCAACAATCTCCCCTCCGCCCTCAAGGTCGTTAACCTCCTCAAATCCCGCGGCCTTCAAAGGGTTAAAGTTTACGACACCGATCCGGCTGTTCTCAGAGCCATTTCCGGCTCTGGCATTAAAGTCACAGTCGATTTGCCCAACGAACTCCTCTTCGCCGCCGCCAAACGCCTCACCTTTGCCTACGCATGGGTCGAAAAGAACATCGTCGCCTACTACCCTTCCACGGAAATCGAAGCCATTGCTGTCGGCAACGAAGTCTTTGTCGACCCACATAACACGACGTCGTTTCTCATTCCGGCCATGAAGAACATTCACCAAGCCCTCGTTAAATACAACCTCCATTCCTCCATTAAGGTCTCTTCTCCTATAGCCTTAAGCGCCCTGCAAAACTCTTACCCTTCCTCCGCCGGTTCATTCCGTCCGGAGCTTATCGAATCGGTTTTCCGGCCAATGCTGGAGTTTCTCCGCCAGACTGGCTCCTATTTGATGGTTAACGCTTACCCATTTTTCGCTTACGAGTCTAACTCCGATGTCATTTCTTTAGACTACGCTCTTTTCCGGGAGAACCCCGGCGTGGTGGATGCCGGTAGCGGTTACCGGTACTTCAGTTTATTCGACGCCCAAATCGACGCCGTTTTCGCCGCCATGTCGGCTCTGAAATACGACGACATTAAAATGGTGGTTACCGAAACTGGGTGGCCGTCCAAAGGCGACGAGAACGAGATTGGCGCTAGTGTCGAAAACGCCGCCGCATACAACGGGAATCTGGTCCGGCGGATTTTGAGCGGCGGCGGAACTCCATTGCGGCCGAAAGCAGATCTGACCGTCTATTTATTCGCCCTTTTCAACGAAAACAAAAAGAACGGTCCCACATCGGAAAGAAACTACGGCTTATTTTACCCTAACGAAGAATCAGTTTACGATATCCCTTTCACAACAGAGGGGTTGAAAGACTACCACGACAAACCATCGCCGTCGCCGTCGCCGGCTACCGGTGGCGACGGGAAGTCCGGACCAGTCCACGGCGATGGGAATGTTTCAAAAAGTCAAACGGGGAATACGTGGTGCGTTGCGAGTGGTGAAGCGGGGAAAGAGAAGCTGCAGGCGAGTCTAGATTATGCTTGTGGTGAAGGAGGGGCGGATTGCCGTCCAATCCAAGTGGGTGCCACGTGTTACAATCCTAACACGCTTGAGGCACATGCTTCGTATGCTTTCAATAGTTATTATCAGAAGAACGGGCGAAAGACCGGCACGTGTTACTTTGGAGGGGCGGCTTACGTAGTCACGCAACCACCGAAGTATGAGAGAATTCTTTTGTAA

Coding sequence (CDS)

ATGGAGCGCCTAACCCTCTCTCTTCTTCTTCAGGCTGGTTCCATTGGTGTCAACTATGGCAGAATCGCCAACAATCTCCCCTCCGCCCTCAAGGTCGTTAACCTCCTCAAATCCCGCGGCCTTCAAAGGGTTAAAGTTTACGACACCGATCCGGCTGTTCTCAGAGCCATTTCCGGCTCTGGCATTAAAGTCACAGTCGATTTGCCCAACGAACTCCTCTTCGCCGCCGCCAAACGCCTCACCTTTGCCTACGCATGGGTCGAAAAGAACATCGTCGCCTACTACCCTTCCACGGAAATCGAAGCCATTGCTGTCGGCAACGAAGTCTTTGTCGACCCACATAACACGACGTCGTTTCTCATTCCGGCCATGAAGAACATTCACCAAGCCCTCGTTAAATACAACCTCCATTCCTCCATTAAGGTCTCTTCTCCTATAGCCTTAAGCGCCCTGCAAAACTCTTACCCTTCCTCCGCCGGTTCATTCCGTCCGGAGCTTATCGAATCGGTTTTCCGGCCAATGCTGGAGTTTCTCCGCCAGACTGGCTCCTATTTGATGGTTAACGCTTACCCATTTTTCGCTTACGAGTCTAACTCCGATGTCATTTCTTTAGACTACGCTCTTTTCCGGGAGAACCCCGGCGTGGTGGATGCCGGTAGCGGTTACCGGTACTTCAGTTTATTCGACGCCCAAATCGACGCCGTTTTCGCCGCCATGTCGGCTCTGAAATACGACGACATTAAAATGGTGGTTACCGAAACTGGGTGGCCGTCCAAAGGCGACGAGAACGAGATTGGCGCTAGTGTCGAAAACGCCGCCGCATACAACGGGAATCTGGTCCGGCGGATTTTGAGCGGCGGCGGAACTCCATTGCGGCCGAAAGCAGATCTGACCGTCTATTTATTCGCCCTTTTCAACGAAAACAAAAAGAACGGTCCCACATCGGAAAGAAACTACGGCTTATTTTACCCTAACGAAGAATCAGTTTACGATATCCCTTTCACAACAGAGGGGTTGAAAGACTACCACGACAAACCATCGCCGTCGCCGTCGCCGGCTACCGGTGGCGACGGGAAGTCCGGACCAGTCCACGGCGATGGGAATGTTTCAAAAAGTCAAACGGGGAATACGTGGTGCGTTGCGAGTGGTGAAGCGGGGAAAGAGAAGCTGCAGGCGAGTCTAGATTATGCTTGTGGTGAAGGAGGGGCGGATTGCCGTCCAATCCAAGTGGGTGCCACGTGTTACAATCCTAACACGCTTGAGGCACATGCTTCGTATGCTTTCAATAGTTATTATCAGAAGAACGGGCGAAAGACCGGCACGTGTTACTTTGGAGGGGCGGCTTACGTAGTCACGCAACCACCGAAGTATGAGAGAATTCTTTTGTAA

Protein sequence

MERLTLSLLLQAGSIGVNYGRIANNLPSALKVVNLLKSRGLQRVKVYDTDPAVLRAISGSGIKVTVDLPNELLFAAAKRLTFAYAWVEKNIVAYYPSTEIEAIAVGNEVFVDPHNTTSFLIPAMKNIHQALVKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPELIESVFRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDAGSGYRYFSLFDAQIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGTPLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDKPSPSPSPATGGDGKSGPVHGDGNVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQVGATCYNPNTLEAHASYAFNSYYQKNGRKTGTCYFGGAAYVVTQPPKYERILL

Homology

[Close]

BLAST of Carg04638-RA vs. NCBI nr
Match: KAG7022968.1 (Glucan endo-1,3-beta-glucosidase 13, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 920.2 bits (2377), Expect = 6.9e-264
Identity = 462/462 (100.00%), Postives = 462/462 (100.00%), Query Frame = 0


Query: 1   MERLTLSLLLQAGSIGVNYGRIANNLPSALKVVNLLKSRGLQRVKVYDTDPAVLRAISGS 60
           MERLTLSLLLQAGSIGVNYGRIANNLPSALKVVNLLKSRGLQRVKVYDTDPAVLRAISGS
Sbjct: 1   MERLTLSLLLQAGSIGVNYGRIANNLPSALKVVNLLKSRGLQRVKVYDTDPAVLRAISGS 60

Query: 61  GIKVTVDLPNELLFAAAKRLTFAYAWVEKNIVAYYPSTEIEAIAVGNEVFVDPHNTTSFL 120
           GIKVTVDLPNELLFAAAKRLTFAYAWVEKNIVAYYPSTEIEAIAVGNEVFVDPHNTTSFL
Sbjct: 61  GIKVTVDLPNELLFAAAKRLTFAYAWVEKNIVAYYPSTEIEAIAVGNEVFVDPHNTTSFL 120

Query: 121 IPAMKNIHQALVKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPELIESVFRPMLEFLRQ 180
           IPAMKNIHQALVKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPELIESVFRPMLEFLRQ
Sbjct: 121 IPAMKNIHQALVKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPELIESVFRPMLEFLRQ 180

Query: 181 TGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDAGSGYRYFSLFDAQIDAVFAAMS 240
           TGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDAGSGYRYFSLFDAQIDAVFAAMS
Sbjct: 181 TGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDAGSGYRYFSLFDAQIDAVFAAMS 240

Query: 241 ALKYDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGTPLRPKADLTVY 300
           ALKYDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGTPLRPKADLTVY
Sbjct: 241 ALKYDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGTPLRPKADLTVY 300

Query: 301 LFALFNENKKNGPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDKPSPSPSPATGGDGKS 360
           LFALFNENKKNGPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDKPSPSPSPATGGDGKS
Sbjct: 301 LFALFNENKKNGPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDKPSPSPSPATGGDGKS 360

Query: 361 GPVHGDGNVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQVGATCYNPNTL 420
           GPVHGDGNVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQVGATCYNPNTL
Sbjct: 361 GPVHGDGNVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQVGATCYNPNTL 420

Query: 421 EAHASYAFNSYYQKNGRKTGTCYFGGAAYVVTQPPKYERILL 463
           EAHASYAFNSYYQKNGRKTGTCYFGGAAYVVTQPPKYERILL
Sbjct: 421 EAHASYAFNSYYQKNGRKTGTCYFGGAAYVVTQPPKYERILL 462

[Close]

BLAST of Carg04638-RA vs. NCBI nr
Match: KAG6589270.1 (Glucan endo-1,3-beta-glucosidase 12, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 897.5 bits (2318), Expect = 4.8e-257
Identity = 454/471 (96.39%), Postives = 456/471 (96.82%), Query Frame = 0


Query: 1   MERLTLS--------------LLLQAGSIGVNYGRIANNLPSALKVVNLLKSRGLQRVKV 60
           MERLTLS              +L +AGSIGVNYGRIANNLPSALKVVNLLKSRGLQRVKV
Sbjct: 1   MERLTLSFFFVFLLLLSASFPVLTEAGSIGVNYGRIANNLPSALKVVNLLKSRGLQRVKV 60

Query: 61  YDTDPAVLRAISGSGIKVTVDLPNELLFAAAKRLTFAYAWVEKNIVAYYPSTEIEAIAVG 120
           YDTDPAVLRAISGSGIKVTVDLPNELLFAAAKRLTFAYAWVEKNIVAYYPSTEIEAIAVG
Sbjct: 61  YDTDPAVLRAISGSGIKVTVDLPNELLFAAAKRLTFAYAWVEKNIVAYYPSTEIEAIAVG 120

Query: 121 NEVFVDPHNTTSFLIPAMKNIHQALVKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPEL 180
           NEVFVDPHNTTSFLIPAMKNIHQALVKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPEL
Sbjct: 121 NEVFVDPHNTTSFLIPAMKNIHQALVKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPEL 180

Query: 181 IESVFRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDAGSGYRYFS 240
           IESVFRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDAGSGYRYFS
Sbjct: 181 IESVFRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDAGSGYRYFS 240

Query: 241 LFDAQIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSG 300
           LFDAQIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSG
Sbjct: 241 LFDAQIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSG 300

Query: 301 GGTPLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDKP 360
           GGTPLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDKP
Sbjct: 301 GGTPLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDKP 360

Query: 361 SPSPSPATGGDGKSGPVHGDGNVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCR 420
           SPSPSPATGGDGKSGPVHGDGNVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCR
Sbjct: 361 SPSPSPATGGDGKSGPVHGDGNVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCR 420

Query: 421 PIQVGATCYNPNTLEAHASYAFNSYYQKNGRKTGTCYFGGAAYVVTQPPKY 458
           PIQVGATCYNPNTLEAHASYAFNSYYQKNGRKTGTCYFGGAAYVVTQPPKY
Sbjct: 421 PIQVGATCYNPNTLEAHASYAFNSYYQKNGRKTGTCYFGGAAYVVTQPPKY 471

[Close]

BLAST of Carg04638-RA vs. NCBI nr
Match: XP_022930867.1 (glucan endo-1,3-beta-glucosidase 12-like [Cucurbita moschata])

HSP 1 Score: 889.0 bits (2296), Expect = 1.7e-254
Identity = 450/471 (95.54%), Postives = 453/471 (96.18%), Query Frame = 0


Query: 1   MERLTLS--------------LLLQAGSIGVNYGRIANNLPSALKVVNLLKSRGLQRVKV 60
           MERLTLS              +L +AGSIGVNYGRIANNLPSALKVVNLLKS GLQRVKV
Sbjct: 1   MERLTLSFFFVFLLLLAASFPVLTEAGSIGVNYGRIANNLPSALKVVNLLKSHGLQRVKV 60

Query: 61  YDTDPAVLRAISGSGIKVTVDLPNELLFAAAKRLTFAYAWVEKNIVAYYPSTEIEAIAVG 120
           YDTDPAVLRAISGSGIKVTVDLPNELLFAAAKRLTFAY WVEKNIVAYYPSTEIEAIAVG
Sbjct: 61  YDTDPAVLRAISGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNIVAYYPSTEIEAIAVG 120

Query: 121 NEVFVDPHNTTSFLIPAMKNIHQALVKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPEL 180
           NEVFVDPHNTTSFLIPAMKNIHQALVKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPEL
Sbjct: 121 NEVFVDPHNTTSFLIPAMKNIHQALVKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPEL 180

Query: 181 IESVFRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDAGSGYRYFS 240
           IESVFRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDAGSGYRYFS
Sbjct: 181 IESVFRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDAGSGYRYFS 240

Query: 241 LFDAQIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSG 300
           LFDAQIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSG
Sbjct: 241 LFDAQIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSG 300

Query: 301 GGTPLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDKP 360
           GGTPLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDKP
Sbjct: 301 GGTPLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDKP 360

Query: 361 SPSPSPATGGDGKSGPVHGDGNVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCR 420
           SPSPSPATGGDGKSGPV+GDGNVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCR
Sbjct: 361 SPSPSPATGGDGKSGPVNGDGNVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCR 420

Query: 421 PIQVGATCYNPNTLEAHASYAFNSYYQKNGRKTGTCYFGGAAYVVTQPPKY 458
           PIQVGATCYNPNTLEAHASYAFNSYYQKNGR TGTCYFGGAAYVVTQPPKY
Sbjct: 421 PIQVGATCYNPNTLEAHASYAFNSYYQKNGRMTGTCYFGGAAYVVTQPPKY 471

[Close]

BLAST of Carg04638-RA vs. NCBI nr
Match: XP_023531393.1 (glucan endo-1,3-beta-glucosidase 12-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 884.4 bits (2284), Expect = 4.2e-253
Identity = 448/472 (94.92%), Postives = 451/472 (95.55%), Query Frame = 0


Query: 1   MERLTLSLLL---------------QAGSIGVNYGRIANNLPSALKVVNLLKSRGLQRVK 60
           MERLTLS L                +AGSIGVNYGRIANNLPSA+KVVNLLKS  LQRVK
Sbjct: 1   MERLTLSFLFVFFLLLLSPSFPVVTKAGSIGVNYGRIANNLPSAVKVVNLLKSHALQRVK 60

Query: 61  VYDTDPAVLRAISGSGIKVTVDLPNELLFAAAKRLTFAYAWVEKNIVAYYPSTEIEAIAV 120
           VYDTDPAVLRAISGSGIKVTVDLPNELLFAAAKRLTFAY WVEKNIVAYYPSTEIEAIAV
Sbjct: 61  VYDTDPAVLRAISGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNIVAYYPSTEIEAIAV 120

Query: 121 GNEVFVDPHNTTSFLIPAMKNIHQALVKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPE 180
           GNEVFVDPHNTTSFLIPAMKNIHQALVKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPE
Sbjct: 121 GNEVFVDPHNTTSFLIPAMKNIHQALVKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPE 180

Query: 181 LIESVFRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDAGSGYRYF 240
           LIESVFRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDAGSGYRYF
Sbjct: 181 LIESVFRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDAGSGYRYF 240

Query: 241 SLFDAQIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILS 300
           SLFDAQIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILS
Sbjct: 241 SLFDAQIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILS 300

Query: 301 GGGTPLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDK 360
           GGGTPLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDK
Sbjct: 301 GGGTPLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDK 360

Query: 361 PSPSPSPATGGDGKSGPVHGDGNVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADC 420
           PSPSPSPATGGDGKSGPV+G GNVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADC
Sbjct: 361 PSPSPSPATGGDGKSGPVNGGGNVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADC 420

Query: 421 RPIQVGATCYNPNTLEAHASYAFNSYYQKNGRKTGTCYFGGAAYVVTQPPKY 458
           RPIQVGATCYNPNTLEAHASYAFNSYYQKNGRKTGTCYFGGAAYVVTQPPKY
Sbjct: 421 RPIQVGATCYNPNTLEAHASYAFNSYYQKNGRKTGTCYFGGAAYVVTQPPKY 472

[Close]

BLAST of Carg04638-RA vs. NCBI nr
Match: XP_022989090.1 (glucan endo-1,3-beta-glucosidase 12-like [Cucurbita maxima])

HSP 1 Score: 879.4 bits (2271), Expect = 1.4e-251
Identity = 445/471 (94.48%), Postives = 451/471 (95.75%), Query Frame = 0


Query: 1   MERLTLS--------------LLLQAGSIGVNYGRIANNLPSALKVVNLLKSRGLQRVKV 60
           MERLTLS              ++ +AGSIGVNYGRIANNLPSA+KVVNLLKS GLQRVKV
Sbjct: 1   MERLTLSFFFVFLLLLSPSFAVVTEAGSIGVNYGRIANNLPSAVKVVNLLKSHGLQRVKV 60

Query: 61  YDTDPAVLRAISGSGIKVTVDLPNELLFAAAKRLTFAYAWVEKNIVAYYPSTEIEAIAVG 120
           YDTDPAVL AISGSGIKVTVDLPNELLFAAAKRLTFAY WVEKNIVAYYPSTEIEAIAVG
Sbjct: 61  YDTDPAVLTAISGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNIVAYYPSTEIEAIAVG 120

Query: 121 NEVFVDPHNTTSFLIPAMKNIHQALVKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPEL 180
           NEVFVDPHNTTSFLIPAMKNIHQALVKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPEL
Sbjct: 121 NEVFVDPHNTTSFLIPAMKNIHQALVKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPEL 180

Query: 181 IESVFRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDAGSGYRYFS 240
           IESVFRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDAGSGYRYFS
Sbjct: 181 IESVFRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDAGSGYRYFS 240

Query: 241 LFDAQIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSG 300
           LFDAQIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASV NAAAYNGNLVRRILSG
Sbjct: 241 LFDAQIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASVGNAAAYNGNLVRRILSG 300

Query: 301 GGTPLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDKP 360
           GGTPLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDKP
Sbjct: 301 GGTPLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDKP 360

Query: 361 SPSPSPATGGDGKSGPVHGDGNVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCR 420
           SPSPSPATGGDGKSGPV+GDGNVSKSQ+GNTWCVASGEAGKEKLQASLDYACGEGGADCR
Sbjct: 361 SPSPSPATGGDGKSGPVNGDGNVSKSQSGNTWCVASGEAGKEKLQASLDYACGEGGADCR 420

Query: 421 PIQVGATCYNPNTLEAHASYAFNSYYQKNGRKTGTCYFGGAAYVVTQPPKY 458
           PIQVGATCYNPNTLEAHASYAFNSYYQKNGRKTGTCYFGGAAYVVTQ PKY
Sbjct: 421 PIQVGATCYNPNTLEAHASYAFNSYYQKNGRKTGTCYFGGAAYVVTQQPKY 471

[Close]

BLAST of Carg04638-RA vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 1.9e-99
Identity = 191/457 (41.79%), Postives = 282/457 (61.71%), Query Frame = 0


Query: 12  AGSIGVNYGRIANNLPSALKVVNLLKSRGLQRVKVYDTDPAVLRAISGSGIKVTVDLPNE 71
           A  IG+ YGR A+NLPS  +V  L++   ++ V++YD +  VL+A + +GI++ + +PN 
Sbjct: 24  ASKIGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANIDVLKAFANTGIELMIGVPNA 83

Query: 72  LLFAAAKRLTFAYAWVEKNIVAYYPSTEIEAIAVGNEVFVDPHNTTSFLIPAMKNIHQAL 131
            L A A+  +    W+  NI+ YYPST+I +I+VG EV   P N T  ++PAM+NIH AL
Sbjct: 84  DLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPDNATGLVLPAMRNIHTAL 143

Query: 132 VKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPELIESVFRPMLEFLRQTGSYLMVNAYP 191
            K  L   IK+SS  +L+ L  S+P S+ SF  +   +  +PMLEFL +  S  M++ YP
Sbjct: 144 KKSGLDKKIKISSSHSLAILSRSFPPSSASFSKK-HSAFLKPMLEFLVENESPFMIDLYP 203

Query: 192 FFAYESNSDVISLDYALFRENPGVVDAGSGYRYFSLFDAQIDAVFAAMSALKYDDIKMVV 251
           ++AY  +++ + L+YALF  +  VVD  +G  Y ++FDAQ+DA++ A++A+ +  +K++V
Sbjct: 204 YYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLDAIYFALTAMSFKTVKVMV 263

Query: 252 TETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGTPLRPKADLTVYLFALFNENKKN 311
           TE+GWPSKG   E  A+ ENA AYN NL+R ++   GTP +P  ++ VYLF+LFNEN+K 
Sbjct: 264 TESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPGEEIDVYLFSLFNENRKP 323

Query: 312 GPTSERNYGLFYPNEESVYDIPFTTEGLKDYH-------DKPSPSPSPATGGDGKSGPVH 371
           G  SERN+G+FY N  +VY + FT E               PSPS SP   G+       
Sbjct: 324 GIESERNWGMFYANGTNVYALDFTGENTTPVSPTNSTTGTSPSPSSSPIINGNSTVTIGG 383

Query: 372 GDGNVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQVGATCYNPNTLEAHA 431
           G G  +K      WC+AS +A   +LQ +LD+ACG G  DC  +Q    C+ P+T+ +HA
Sbjct: 384 GGGGGTKK-----WCIASSQASVTELQTALDWACGPGNVDCSAVQPDQPCFEPDTVLSHA 443

Query: 432 SYAFNSYYQKNGRKTGTCYFGGAAYVVTQPPKYERIL 462
           SYAFN+YYQ++G  +  C F GA+  V + P Y   L
Sbjct: 444 SYAFNTYYQQSGASSIDCSFNGASVEVDKDPSYGNCL 474

[Close]

BLAST of Carg04638-RA vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 6.8e-97
Identity = 183/452 (40.49%), Postives = 278/452 (61.50%), Query Frame = 0


Query: 13  GSIGVNYGRIANNLPSALKVVNLLKSRGLQRVKVYDTDPAVLRAISGSGIKVTVDLPNEL 72
           G +GV YGR A++LP+  KVV L++   ++ V++YD +  VL+A   + I++ + +PN  
Sbjct: 24  GKVGVCYGRSADDLPTPSKVVQLIQQHNIKYVRIYDYNSQVLKAFGNTSIELMIGVPNSD 83

Query: 73  LFAAAKRLTFAYAWVEKNIVAYYPSTEIEAIAVGNEVFVDPH-NTTSFLIPAMKNIHQAL 132
           L A ++  +    W++ +++ YYP+T+I  I VG E   DPH N +SF++PAM+N+  AL
Sbjct: 84  LNAFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAESTDDPHINASSFVVPAMQNVLTAL 143

Query: 133 VKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPELIESVFRPMLEFLRQTGSYLMVNAYP 192
            K  L   IKVS+ ++L  L  S+P SAG+F         RPMLEFL +  S  M++ YP
Sbjct: 144 RKVGLSRRIKVSTTLSLGILSRSFPPSAGAFNSS-YAYFLRPMLEFLAENKSPFMIDLYP 203

Query: 193 FFAYESNSDVISLDYALFRENPGVVDAGSGYRYFSLFDAQIDAVFAAMSALKYDDIKMVV 252
           ++AY  + + +SLDY LF  +  V+D  +G  Y ++FDAQ+DA++ A++AL +  IK++V
Sbjct: 204 YYAYRDSPNNVSLDYVLFESSSEVIDPNTGLLYKNMFDAQVDALYYALTALNFRTIKIMV 263

Query: 253 TETGWPSKGDENE-IGASVENAAAYNGNLVRRILSGGGTPLRPKADLTVYLFALFNENKK 312
           TETGWP+KG   E   AS +NA  YN N++R +++  GTP +P   + VY+F+LFNEN+K
Sbjct: 264 TETGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQGTPAKPGEAMNVYIFSLFNENRK 323

Query: 313 NGPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDKPSPSPSPATGGDGKSGPVHGDGN-V 372
            G  SERN+GLFYP++ SVY + FT                      GKS   H + +  
Sbjct: 324 AGLDSERNWGLFYPDQTSVYQLDFT----------------------GKSNGFHSNSSGT 383

Query: 373 SKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQVGATCYNPNTLEAHASYAFN 432
           + S + N+WC+AS +A +  L+ +LD+ACG G  DC  IQ    C+ P+TL +HAS+ FN
Sbjct: 384 NSSGSSNSWCIASSKASERDLKGALDWACGPGNVDCTAIQPSQPCFQPDTLVSHASFVFN 443

Query: 433 SYYQKNGRKTGTCYFGGAAYVVTQPPKYERIL 462
           SY+Q+N      C FGGA   V + P Y++ +
Sbjct: 444 SYFQQNRATDVACSFGGAGVKVNKDPSYDKCI 452

[Close]

BLAST of Carg04638-RA vs. ExPASy Swiss-Prot
Match: Q9ZU91 (Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 PE=2 SV=2)

HSP 1 Score: 335.5 bits (859), Expect = 9.5e-91
Identity = 182/452 (40.27%), Postives = 266/452 (58.85%), Query Frame = 0


Query: 7   SLLLQAGSIGVNYGRIANNLPSALKVVNLLKSRGLQRVKVYDTDPAVLRAISGSGIKVTV 66
           S L Q   IGVN G    N+PS  +VV LLKS+ + RV++YD D ++L A + +G++V +
Sbjct: 15  SALSQDSLIGVNIGTEVTNMPSPTQVVALLKSQNINRVRLYDADRSMLLAFAHTGVQVII 74

Query: 67  DLPNELLFAAAKRLTFAYAWVEKNIVAYYPSTEIEAIAVGNEVFVDPHNTTSFLIPAMKN 126
            +PN+ L   ++    A  WV +N+ AYYP+T I  IAVG+EV     N  S L+ A+K 
Sbjct: 75  SVPNDQLLGISQSNATAANWVTRNVAAYYPATNITTIAVGSEVLTSLTNAASVLVSALKY 134

Query: 127 IHQALVKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPELIESVFRPMLEFLRQTGSYLM 186
           I  ALV  NL   IKVS+P + + + +S+P S   F  +  + V  P+L+FL+ TGS L+
Sbjct: 135 IQAALVTANLDRQIKVSTPHSSTIILDSFPPSQAFFN-KTWDPVIVPLLKFLQSTGSPLL 194

Query: 187 VNAYPFFAYESNSDVISLDYALF---RENPGVVDAGSGYRYFSLFDAQIDAVFAAMSALK 246
           +N YP+F Y  ++ VI LDYALF   + N   VDA +   Y ++FDA +DA + AMS L 
Sbjct: 195 LNVYPYFDYVQSNGVIPLDYALFQPLQANKEAVDANTLLHYTNVFDAIVDAAYFAMSYLN 254

Query: 247 YDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGTPLRPKADLTVYLFA 306
           + +I +VVTE+GWPSKG  +E  A+VENA  YN NL++ +++  GTP  P   +T Y++ 
Sbjct: 255 FTNIPIVVTESGWPSKGGPSEHDATVENANTYNSNLIQHVINKTGTPKHPGTAVTTYIYE 314

Query: 307 LFNENKKNGPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDKPSPSPSPATGGDGKSGPV 366
           L+NE+ + GP SE+N+GLFY N   VY +     G                         
Sbjct: 315 LYNEDTRPGPVSEKNWGLFYTNGTPVYTLRLAGAG------------------------- 374

Query: 367 HGDGNVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQVGATCYNPNTLEAH 426
                ++   T  T+C+A  +  ++ LQA+LD+ACG G  DC  +  G +CY P+ + AH
Sbjct: 375 ---AILANDTTNQTFCIAKEKVDRKMLQAALDWACGPGKVDCSALMQGESCYEPDDVVAH 434

Query: 427 ASYAFNSYYQKNGRKTGTCYFGGAAYVVTQPP 456
           ++YAFN+YYQK G+ +G+C F G A V T  P
Sbjct: 435 STYAFNAYYQKMGKASGSCDFKGVATVTTTDP 437

[Close]

BLAST of Carg04638-RA vs. ExPASy Swiss-Prot
Match: Q9C7U5 (Glucan endo-1,3-beta-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66250 PE=2 SV=2)

HSP 1 Score: 333.2 bits (853), Expect = 4.7e-90
Identity = 182/468 (38.89%), Postives = 273/468 (58.33%), Query Frame = 0


Query: 4   LTLSLLLQAGS-----------IGVNYGRIANNLPSALKVVNLLKSRGLQRVKVYDTDPA 63
           L+LSLL+ A +           IGVN G   +++P   +VV LLK++ ++ +++Y+ DP 
Sbjct: 10  LSLSLLVLASASPSPPADEGSYIGVNIGTDLSDMPHPTQVVALLKAQEIRHIRLYNADPG 69

Query: 64  VLRAISGSGIKVTVDLPNELLFAAAKRLTFAYAWVEKNIVAYYPSTEIEAIAVGNEVFVD 123
           +L A++ +GIKV + +PN+ L    +  + A  WV++N++A+YP+T I A++VG+EV   
Sbjct: 70  LLIALANTGIKVIISIPNDQLLGIGQSNSTAANWVKRNVIAHYPATMITAVSVGSEVLTS 129

Query: 124 PHNTTSFLIPAMKNIHQALVKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPELIESVFR 183
             N    L+ A+KN+H AL+  NL   IKVS+P++ S + + +P S   F   L  +V  
Sbjct: 130 LSNAAPVLVSAIKNVHAALLSANLDKLIKVSTPLSTSLILDPFPPSQAFFNRSL-NAVIV 189

Query: 184 PMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFR---ENPGVVDAGSGYRYFSLFD 243
           P+L FL+ T SYLMVN YP+  Y  ++ VI LDYALF+    N   VDA +  RY + FD
Sbjct: 190 PLLSFLQSTNSYLMVNVYPYIDYMQSNGVIPLDYALFKPIPPNKEAVDANTLVRYSNAFD 249

Query: 244 AQIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGT 303
           A +DA + AM+ L + +I ++VTE+GWPSKG+ NE  A+++NA  YN NL+R +L+  GT
Sbjct: 250 AMVDATYFAMAFLNFTNIPVLVTESGWPSKGETNEPDATLDNANTYNSNLIRHVLNKTGT 309

Query: 304 PLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDKPSPS 363
           P RP   ++ Y++ L+NE+ K G  SE+N+GLF  N E VY +  T  G           
Sbjct: 310 PKRPGIAVSTYIYELYNEDTKAG-LSEKNWGLFNANGEPVYVLRLTNSG----------- 369

Query: 364 PSPATGGDGKSGPVHGDGNVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQ 423
                              ++   T  T+C A   A  + LQA+LD+ACG G  DC PI+
Sbjct: 370 -----------------SVLANDTTNQTYCTAREGADTKMLQAALDWACGPGKIDCSPIK 429

Query: 424 VGATCYNPNTLEAHASYAFNSYYQKNGRKTGTCYFGGAAYVVTQPPKY 458
            G TCY P+ + AHA+YAF++YY + G     C F G A + T  P +
Sbjct: 430 QGETCYEPDNVVAHANYAFDTYYHQTGNNPDACNFNGVASITTTDPSH 447

[Close]

BLAST of Carg04638-RA vs. ExPASy Swiss-Prot
Match: Q94CD8 (Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 PE=2 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 2.4e-89
Identity = 180/459 (39.22%), Postives = 269/459 (58.61%), Query Frame = 0


Query: 4   LTLSLLL--QAGSIGVNYGRIANNLPSALKVVNLLKSRGLQRVKVYDTDPAVLRAISGSG 63
           L LS+L    A  IGVN G    N+P    +V LLKS+ +  V++YD +  +L+A + + 
Sbjct: 13  LLLSILACSNAAFIGVNIGTDLTNMPPPSDIVTLLKSQQITHVRLYDANSHMLKAFANTS 72

Query: 64  IKVTVDLPNELLFAAAKRLTFAYAWVEKNIVAYYPSTEIEAIAVGNEVFVDPHNTTSFLI 123
           I+V V + NE +    +  + A AWV KN+ AY PST I AIAVG+EV     +    L 
Sbjct: 73  IEVMVGVTNEEILKIGRFPSAAAAWVNKNVAAYIPSTNITAIAVGSEVLTTIPHVAPILA 132

Query: 124 PAMKNIHQALVKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPELIESVFRPMLEFLRQT 183
            A+ NIH+ALV  NL+  +KVSSP+++  +   +P S  +F P    +V++ +L+FL+ T
Sbjct: 133 SALNNIHKALVASNLNFKVKVSSPMSMDIMPKPFPPSTSTFSPSWNTTVYQ-LLQFLKNT 192

Query: 184 GSYLMVNAYPFFAYESNSDVISLDYALFRE-NP--GVVDAGSGYRYFSLFDAQIDAVFAA 243
           GS+ M+NAYP++ Y + + +  LDYALF++ +P   +VD  +   Y S+FDA +DA + +
Sbjct: 193 GSFFMLNAYPYYGYTTANGIFPLDYALFKQLSPVKQIVDPNTLLHYNSMFDAMVDAAYYS 252

Query: 244 MSALKYDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGTPLRPKADLT 303
           M AL +  I +VVTETGWPS G  +E  A+V NA  +N NL++R+L+  G P +P   + 
Sbjct: 253 MEALNFSKIPVVVTETGWPSSGGSDEAAATVANAETFNTNLIKRVLNNSGPPSQPDIPIN 312

Query: 304 VYLFALFNENKKNGPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDKPSPSPSPATGGDG 363
            Y++ L+NE+K++GP SERN+G+ +PN  SVY +                    +  G  
Sbjct: 313 TYIYELYNEDKRSGPVSERNWGILFPNGTSVYPL--------------------SLSGGS 372

Query: 364 KSGPVHGDGNVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQVGATCYNPN 423
            S  ++G          + +CVA  +A  +KL   L++ACG+G A+C  IQ G  CY PN
Sbjct: 373 SSAALNG---------SSMFCVAKADADDDKLVDGLNWACGQGRANCAAIQPGQPCYLPN 432

Query: 424 TLEAHASYAFNSYYQKNGRKTGTCYFGGAAYVVTQPPKY 458
            +++HAS+AFN YYQK     GTC F G A   T+ P Y
Sbjct: 433 DVKSHASFAFNDYYQKMKSAGGTCDFDGTAITTTRDPSY 441

[Close]

BLAST of Carg04638-RA vs. ExPASy TrEMBL
Match: A0A6J1ERV0 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111437224 PE=3 SV=1)

HSP 1 Score: 889.0 bits (2296), Expect = 8.3e-255
Identity = 450/471 (95.54%), Postives = 453/471 (96.18%), Query Frame = 0


Query: 1   MERLTLS--------------LLLQAGSIGVNYGRIANNLPSALKVVNLLKSRGLQRVKV 60
           MERLTLS              +L +AGSIGVNYGRIANNLPSALKVVNLLKS GLQRVKV
Sbjct: 1   MERLTLSFFFVFLLLLAASFPVLTEAGSIGVNYGRIANNLPSALKVVNLLKSHGLQRVKV 60

Query: 61  YDTDPAVLRAISGSGIKVTVDLPNELLFAAAKRLTFAYAWVEKNIVAYYPSTEIEAIAVG 120
           YDTDPAVLRAISGSGIKVTVDLPNELLFAAAKRLTFAY WVEKNIVAYYPSTEIEAIAVG
Sbjct: 61  YDTDPAVLRAISGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNIVAYYPSTEIEAIAVG 120

Query: 121 NEVFVDPHNTTSFLIPAMKNIHQALVKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPEL 180
           NEVFVDPHNTTSFLIPAMKNIHQALVKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPEL
Sbjct: 121 NEVFVDPHNTTSFLIPAMKNIHQALVKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPEL 180

Query: 181 IESVFRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDAGSGYRYFS 240
           IESVFRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDAGSGYRYFS
Sbjct: 181 IESVFRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDAGSGYRYFS 240

Query: 241 LFDAQIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSG 300
           LFDAQIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSG
Sbjct: 241 LFDAQIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSG 300

Query: 301 GGTPLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDKP 360
           GGTPLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDKP
Sbjct: 301 GGTPLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDKP 360

Query: 361 SPSPSPATGGDGKSGPVHGDGNVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCR 420
           SPSPSPATGGDGKSGPV+GDGNVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCR
Sbjct: 361 SPSPSPATGGDGKSGPVNGDGNVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCR 420

Query: 421 PIQVGATCYNPNTLEAHASYAFNSYYQKNGRKTGTCYFGGAAYVVTQPPKY 458
           PIQVGATCYNPNTLEAHASYAFNSYYQKNGR TGTCYFGGAAYVVTQPPKY
Sbjct: 421 PIQVGATCYNPNTLEAHASYAFNSYYQKNGRMTGTCYFGGAAYVVTQPPKY 471

[Close]

BLAST of Carg04638-RA vs. ExPASy TrEMBL
Match: A0A6J1JLE7 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111486266 PE=3 SV=1)

HSP 1 Score: 879.4 bits (2271), Expect = 6.6e-252
Identity = 445/471 (94.48%), Postives = 451/471 (95.75%), Query Frame = 0


Query: 1   MERLTLS--------------LLLQAGSIGVNYGRIANNLPSALKVVNLLKSRGLQRVKV 60
           MERLTLS              ++ +AGSIGVNYGRIANNLPSA+KVVNLLKS GLQRVKV
Sbjct: 1   MERLTLSFFFVFLLLLSPSFAVVTEAGSIGVNYGRIANNLPSAVKVVNLLKSHGLQRVKV 60

Query: 61  YDTDPAVLRAISGSGIKVTVDLPNELLFAAAKRLTFAYAWVEKNIVAYYPSTEIEAIAVG 120
           YDTDPAVL AISGSGIKVTVDLPNELLFAAAKRLTFAY WVEKNIVAYYPSTEIEAIAVG
Sbjct: 61  YDTDPAVLTAISGSGIKVTVDLPNELLFAAAKRLTFAYTWVEKNIVAYYPSTEIEAIAVG 120

Query: 121 NEVFVDPHNTTSFLIPAMKNIHQALVKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPEL 180
           NEVFVDPHNTTSFLIPAMKNIHQALVKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPEL
Sbjct: 121 NEVFVDPHNTTSFLIPAMKNIHQALVKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPEL 180

Query: 181 IESVFRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDAGSGYRYFS 240
           IESVFRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDAGSGYRYFS
Sbjct: 181 IESVFRPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDAGSGYRYFS 240

Query: 241 LFDAQIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSG 300
           LFDAQIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASV NAAAYNGNLVRRILSG
Sbjct: 241 LFDAQIDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASVGNAAAYNGNLVRRILSG 300

Query: 301 GGTPLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDKP 360
           GGTPLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDKP
Sbjct: 301 GGTPLRPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDKP 360

Query: 361 SPSPSPATGGDGKSGPVHGDGNVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCR 420
           SPSPSPATGGDGKSGPV+GDGNVSKSQ+GNTWCVASGEAGKEKLQASLDYACGEGGADCR
Sbjct: 361 SPSPSPATGGDGKSGPVNGDGNVSKSQSGNTWCVASGEAGKEKLQASLDYACGEGGADCR 420

Query: 421 PIQVGATCYNPNTLEAHASYAFNSYYQKNGRKTGTCYFGGAAYVVTQPPKY 458
           PIQVGATCYNPNTLEAHASYAFNSYYQKNGRKTGTCYFGGAAYVVTQ PKY
Sbjct: 421 PIQVGATCYNPNTLEAHASYAFNSYYQKNGRKTGTCYFGGAAYVVTQQPKY 471

[Close]

BLAST of Carg04638-RA vs. ExPASy TrEMBL
Match: A0A6J1I0V3 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111468418 PE=3 SV=1)

HSP 1 Score: 833.6 bits (2152), Expect = 4.1e-238
Identity = 417/466 (89.48%), Postives = 436/466 (93.56%), Query Frame = 0


Query: 1   MERLTLSLLL---------QAGSIGVNYGRIANNLPSALKVVNLLKSRGLQRVKVYDTDP 60
           MERLTLS  +         ++GSIGVNYGRIANNLPSA+KVV LLKS GLQRVKVYDTDP
Sbjct: 1   MERLTLSFFIFLLFLYPFAESGSIGVNYGRIANNLPSAVKVVKLLKSHGLQRVKVYDTDP 60

Query: 61  AVLRAISGSGIKVTVDLPNELLFAAAKRLTFAYAWVEKNIVAYYPSTEIEAIAVGNEVFV 120
           AVLRA+SGSGIKVTVDLPNELLFAAAKRL+FAY WVEKN+ AYYPSTEIEAIAVGNEVFV
Sbjct: 61  AVLRALSGSGIKVTVDLPNELLFAAAKRLSFAYTWVEKNVAAYYPSTEIEAIAVGNEVFV 120

Query: 121 DPHNTTSFLIPAMKNIHQALVKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPELIESVF 180
           DPHNTTSFL+PAMKNIHQALVK+NLHS+IKVSSPIALSALQNSYPSSAGSFRPEL+E VF
Sbjct: 121 DPHNTTSFLVPAMKNIHQALVKHNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVEPVF 180

Query: 181 RPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDAGSGYRYFSLFDAQ 240
           RPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVD+GSGYRYF+LFDAQ
Sbjct: 181 RPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDSGSGYRYFNLFDAQ 240

Query: 241 IDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGTPL 300
           IDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGAS  NAAAYNGNL+RRILSGGGTPL
Sbjct: 241 IDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASAANAAAYNGNLIRRILSGGGTPL 300

Query: 301 RPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDKPSPSPS 360
           RPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEE VYDIPFTTE LKDYHDK  PSP+
Sbjct: 301 RPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEERVYDIPFTTEELKDYHDK--PSPA 360

Query: 361 PATGGDGKSGPVHGDGNVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQVG 420
           PATGGDG++G  HGDG VSKSQTGNTWCVASGEAGKEKLQA LDYACGEGGADCRPIQ  
Sbjct: 361 PATGGDGQTGSGHGDGGVSKSQTGNTWCVASGEAGKEKLQAGLDYACGEGGADCRPIQPS 420

Query: 421 ATCYNPNTLEAHASYAFNSYYQKNGRKTGTCYFGGAAYVVTQPPKY 458
           ATCYNPNTL+AHASYAFNSYYQKN RK GTCYFGGAAYVVTQPPK+
Sbjct: 421 ATCYNPNTLQAHASYAFNSYYQKNVRKIGTCYFGGAAYVVTQPPKF 464

[Close]

BLAST of Carg04638-RA vs. ExPASy TrEMBL
Match: A0A6J1GMW6 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111455410 PE=3 SV=1)

HSP 1 Score: 829.7 bits (2142), Expect = 6.0e-237
Identity = 415/466 (89.06%), Postives = 436/466 (93.56%), Query Frame = 0


Query: 1   MERLTLSLLL---------QAGSIGVNYGRIANNLPSALKVVNLLKSRGLQRVKVYDTDP 60
           MERLTLS  +         ++GSIGVNYGRIAN+LPSA+KVV LLKS GLQRVKVYDTDP
Sbjct: 1   MERLTLSFFIFLLFLSPFAESGSIGVNYGRIANDLPSAVKVVKLLKSHGLQRVKVYDTDP 60

Query: 61  AVLRAISGSGIKVTVDLPNELLFAAAKRLTFAYAWVEKNIVAYYPSTEIEAIAVGNEVFV 120
           AVLRA+SGSGIKVTVDLPNELLFAAAKRL+FAY WVEKN+ AYYPSTEIEAIAVGNEVFV
Sbjct: 61  AVLRALSGSGIKVTVDLPNELLFAAAKRLSFAYTWVEKNVAAYYPSTEIEAIAVGNEVFV 120

Query: 121 DPHNTTSFLIPAMKNIHQALVKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPELIESVF 180
           DPHNTTSFL+PAMKNIHQALVK+NLHS+IKVSSPIALSALQNSYPSSAGSFRPEL+E VF
Sbjct: 121 DPHNTTSFLVPAMKNIHQALVKHNLHSAIKVSSPIALSALQNSYPSSAGSFRPELVEPVF 180

Query: 181 RPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDAGSGYRYFSLFDAQ 240
           RPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVD+GSGYRYF+LFDAQ
Sbjct: 181 RPMLEFLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDSGSGYRYFNLFDAQ 240

Query: 241 IDAVFAAMSALKYDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGTPL 300
           IDAVFAAMSALKYDDIK+VVTETGWPSKGDENEIGASV NAAAYNGNL+RRILSGGGTPL
Sbjct: 241 IDAVFAAMSALKYDDIKLVVTETGWPSKGDENEIGASVANAAAYNGNLIRRILSGGGTPL 300

Query: 301 RPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDKPSPSPS 360
           RPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEE VYDIPFTTE LKDYHDK  PSP+
Sbjct: 301 RPKADLTVYLFALFNENKKNGPTSERNYGLFYPNEERVYDIPFTTEELKDYHDK--PSPA 360

Query: 361 PATGGDGKSGPVHGDGNVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQVG 420
           PATGGDG++G   GDG VSKSQTGNTWCVASGEAGKEKLQA LDYACGEGGADCRPIQ  
Sbjct: 361 PATGGDGQAGSGSGDGGVSKSQTGNTWCVASGEAGKEKLQAGLDYACGEGGADCRPIQPS 420

Query: 421 ATCYNPNTLEAHASYAFNSYYQKNGRKTGTCYFGGAAYVVTQPPKY 458
           ATCYNPNTL+AHASYAFNSYYQKN RK GTCYFGGAAYVVTQPPK+
Sbjct: 421 ATCYNPNTLQAHASYAFNSYYQKNARKIGTCYFGGAAYVVTQPPKF 464

[Close]

BLAST of Carg04638-RA vs. ExPASy TrEMBL
Match: A0A0A0KEL0 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_6G421570 PE=3 SV=1)

HSP 1 Score: 824.7 bits (2129), Expect = 1.9e-235
Identity = 411/455 (90.33%), Postives = 430/455 (94.51%), Query Frame = 0


Query: 4   LTLSLLLQAGSIGVNYGRIANNLPSALKVVNLLKSRGLQRVKVYDTDPAVLRAISGSGIK 63
           L LS   ++GSIGVNYGRI N+LPSA+KVV LLKS GLQRVKVYDTDPAVL+A+SGSGIK
Sbjct: 15  LFLSPFAESGSIGVNYGRIGNDLPSAVKVVKLLKSHGLQRVKVYDTDPAVLKALSGSGIK 74

Query: 64  VTVDLPNELLFAAAKRLTFAYAWVEKNIVAYYPSTEIEAIAVGNEVFVDPHNTTSFLIPA 123
           VTVDLPNELLFAAAKRLTFAY WVEKN+ AYYPSTEIEAIAVGNEVFVDPHNTTSFL+PA
Sbjct: 75  VTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTSFLVPA 134

Query: 124 MKNIHQALVKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPELIESVFRPMLEFLRQTGS 183
           MKNIHQALVKYNLHS+IKVSSPIALSALQNSYPSSAGSFRPEL+E+VFRPMLEFLRQTGS
Sbjct: 135 MKNIHQALVKYNLHSNIKVSSPIALSALQNSYPSSAGSFRPELVETVFRPMLEFLRQTGS 194

Query: 184 YLMVNAYPFFAYESNSDVISLDYALFRENPGVVDAGSGYRYFSLFDAQIDAVFAAMSALK 243
           YLMVNAYPFFAYESN+DVISLDYALFR+NPGVVDAGSGYRYF+LFDAQIDAVFAAMSALK
Sbjct: 195 YLMVNAYPFFAYESNTDVISLDYALFRDNPGVVDAGSGYRYFNLFDAQIDAVFAAMSALK 254

Query: 244 YDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGTPLRPKADLTVYLFA 303
           YDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGTPLRPKADLTVYLFA
Sbjct: 255 YDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGTPLRPKADLTVYLFA 314

Query: 304 LFNENKKNGPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDKPSPSPSPATGGDGKSG-P 363
           LFNENKKNGPTSERNYGLFYPNEE VYDIPFTTEGLKD+ DK  PSP P +GG+  +  P
Sbjct: 315 LFNENKKNGPTSERNYGLFYPNEEKVYDIPFTTEGLKDFEDK--PSPKPVSGGNAPTAPP 374

Query: 364 VHGDGNVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQVGATCYNPNTLEA 423
             GDG VSKSQTGNTWCVASGEAGKEKLQ+ LDYACGEGGADCRPIQVGATCYNPNTLEA
Sbjct: 375 ASGDGGVSKSQTGNTWCVASGEAGKEKLQSGLDYACGEGGADCRPIQVGATCYNPNTLEA 434

Query: 424 HASYAFNSYYQKNGRKTGTCYFGGAAYVVTQPPKY 458
           HASYAFNSYYQKN RK GTCYFGGAAYVVTQPPKY
Sbjct: 435 HASYAFNSYYQKNSRKVGTCYFGGAAYVVTQPPKY 467

[Close]

BLAST of Carg04638-RA vs. TAIR 10
Match: AT2G05790.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 701.0 bits (1808), Expect = 6.1e-202
Identity = 342/451 (75.83%), Postives = 399/451 (88.47%), Query Frame = 0


Query: 9   LLQAGSIGVNYGRIANNLPSALKVVNLLKSRGLQRVKVYDTDPAVLRAISGSGIKVTVDL 68
           L  AGSIGVNYGRI++ LPSA KVV LLKS+G+ RVK++D DP+VL+A+SGSGIKVTVDL
Sbjct: 19  LSDAGSIGVNYGRISDELPSAFKVVQLLKSQGITRVKIFDADPSVLKALSGSGIKVTVDL 78

Query: 69  PNELLFAAAKRLTFAYAWVEKNIVAYYPSTEIEAIAVGNEVFVDPHNTTSFLIPAMKNIH 128
           PNELLF+AAKR +FA +WV++N+ AY+PST+IE+IAVGNEVFVD HNTTSFLIPAM+NIH
Sbjct: 79  PNELLFSAAKRTSFAVSWVKRNVAAYHPSTQIESIAVGNEVFVDTHNTTSFLIPAMRNIH 138

Query: 129 QALVKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPELIESVFRPMLEFLRQTGSYLMVN 188
           +AL+ +NLHS IK+SSP+ALSALQNSYPSS+GSFRPELI+SV +PML+FLR+TGS LM+N
Sbjct: 139 KALMSFNLHSDIKISSPLALSALQNSYPSSSGSFRPELIDSVIKPMLDFLRETGSRLMIN 198

Query: 189 AYPFFAYESNSDVISLDYALFRENPGVVDAGSGYRYFSLFDAQIDAVFAAMSALKYDDIK 248
            YPFFAYE NSDVI LDYAL RENPG+VD+G+G RYF+LFDAQIDAVFAAMSALKYDDI+
Sbjct: 199 VYPFFAYEGNSDVIPLDYALLRENPGMVDSGNGLRYFNLFDAQIDAVFAAMSALKYDDIE 258

Query: 249 MVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGTPLRPKADLTVYLFALFNEN 308
           ++VTETGWPSKGDENE+GA++ NAA+YNGNL+RRIL+ GGTPLRPKADLTVYLFALFNEN
Sbjct: 259 IIVTETGWPSKGDENEVGATLANAASYNGNLIRRILTRGGTPLRPKADLTVYLFALFNEN 318

Query: 309 KKNGPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDKPSPSPSPATGGDGKSGPVHGDGN 368
           KK GPTSERNYGLF+P+E+ VYDIPFTTEGLK Y D      +P TGGD  + P    G 
Sbjct: 319 KKLGPTSERNYGLFFPDEKKVYDIPFTTEGLKHYRD---GGHTPVTGGDQVTKPPM-SGG 378

Query: 369 VSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQVGATCYNPNTLEAHASYAF 428
           VSKS  G TWCVA+G+AG+E+LQ  LDYACGEGGADCRPIQ GA CY+P+TLEAHAS+AF
Sbjct: 379 VSKSLNGYTWCVANGDAGEERLQGGLDYACGEGGADCRPIQPGANCYSPDTLEAHASFAF 438

Query: 429 NSYYQKNGRKTGTCYFGGAAYVVTQPPKYER 460
           NSYYQK GR  G+CYFGGAAYVV+QPPKY R
Sbjct: 439 NSYYQKKGRAGGSCYFGGAAYVVSQPPKYGR 465

[Close]

BLAST of Carg04638-RA vs. TAIR 10
Match: AT5G55180.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 585.1 bits (1507), Expect = 4.9e-167
Identity = 295/450 (65.56%), Postives = 349/450 (77.56%), Query Frame = 0


Query: 12  AGSIGVNYGRIANNLPSALKVVNLLKSRGLQRVKVYDTDPAVLRAISGSGIKVTVDLPNE 71
           +G IGVNYGRIA+NLP+  KVV LLK++G+ R+K+YDT+  VL A++ SGIKV V LPNE
Sbjct: 24  SGMIGVNYGRIADNLPAPEKVVELLKTQGINRIKLYDTETTVLTALANSGIKVVVSLPNE 83

Query: 72  LLFAAAKRLTFAYAWVEKNIVAYYPSTEIEAIAVGNEVFVDPHNTTSFLIPAMKNIHQAL 131
            L +AA   ++   WV+ NI  Y P+T+IEAIAVGNEVFVDP NTT++L+PAMKN+  +L
Sbjct: 84  NLASAAADQSYTDTWVQDNIKKYIPATDIEAIAVGNEVFVDPRNTTTYLVPAMKNVQSSL 143

Query: 132 VKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPELIESVFRPMLEFLRQTGSYLMVNAYP 191
           VK+NL  SIK+SSPIALSAL +SYP SAGSF+PELIE V +PML+ LR+T S+LMVNAYP
Sbjct: 144 VKFNLDKSIKISSPIALSALASSYPPSAGSFKPELIEPVIKPMLDLLRKTSSHLMVNAYP 203

Query: 192 FFAYESNSDVISLDYALFRENPGVVDAGSGYRYFSLFDAQIDAVFAAMSALKYDDIKMVV 251
           FFAY +N+D ISLDYALF+EN G VD+G+G +Y SL DAQIDAVFAAMSA+ ++D+K+VV
Sbjct: 204 FFAYAANADKISLDYALFKENAGNVDSGNGLKYNSLLDAQIDAVFAAMSAVGFNDVKLVV 263

Query: 252 TETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGTPLRPKADLTVYLFALFNENKKN 311
           TETGWPS GDENEIGA   NAAAYNG LV+R+L+G GTPL+PK  L VYLFALFNEN+K 
Sbjct: 264 TETGWPSAGDENEIGAGSANAAAYNGGLVKRVLTGNGTPLKPKEPLNVYLFALFNENQKT 323

Query: 312 GPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDKPSPSPSPATGGDGKSGPVHGDGN--- 371
           GPTSERNYGLFYPNE  VYD+                        +GKS PV+ +     
Sbjct: 324 GPTSERNYGLFYPNENKVYDVSL----------------------NGKSTPVNDNKEKVV 383

Query: 372 -VSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQVGATCYNPNTLEAHASYA 431
            V  S  G TWCVA+G+  KEKLQ  LDYACGEGGADCRPIQ GATCYNP +LEAHASYA
Sbjct: 384 PVKPSLVGQTWCVANGKTTKEKLQEGLDYACGEGGADCRPIQPGATCYNPESLEAHASYA 443

Query: 432 FNSYYQKNGRKTGTCYFGGAAYVVTQPPKY 458
           FNSYYQKN R  GTC FGGAAYVV+QPPKY
Sbjct: 444 FNSYYQKNARGVGTCNFGGAAYVVSQPPKY 451

[Close]

BLAST of Carg04638-RA vs. TAIR 10
Match: AT4G26830.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 582.4 bits (1500), Expect = 3.2e-166
Identity = 293/446 (65.70%), Postives = 352/446 (78.92%), Query Frame = 0


Query: 12  AGSIGVNYGRIANNLPSALKVVNLLKSRGLQRVKVYDTDPAVLRAISGSGIKVTVDLPNE 71
           +G +GVNYGRIANNLPS  KVVNLLKS+G+ R+K++DTD  VL A++ S IKV V LPNE
Sbjct: 22  SGMVGVNYGRIANNLPSPEKVVNLLKSQGINRIKIFDTDKNVLTALANSKIKVIVALPNE 81

Query: 72  LLFAAAKRLTFAYAWVEKNIVAYYPSTEIEAIAVGNEVFVDPHNTTSFLIPAMKNIHQAL 131
           LL +AA   +FA  W++ +I+ Y+P+TEIEAIAVGNEVFVDP   T +L+ AMKNIH +L
Sbjct: 82  LLSSAASHQSFADNWIKTHIMPYFPATEIEAIAVGNEVFVDP-TITPYLVNAMKNIHTSL 141

Query: 132 VKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPELIESVFRPMLEFLRQTGSYLMVNAYP 191
           VKY L  +IK+SSPIALSAL NSYP S+GSF+PELIE V +PML  L+QT SYLMVNAYP
Sbjct: 142 VKYKLDKAIKISSPIALSALANSYPPSSGSFKPELIEPVVKPMLALLQQTSSYLMVNAYP 201

Query: 192 FFAYESNSDVISLDYALFRENPGVVDAGSGYRYFSLFDAQIDAVFAAMSALKYDDIKMVV 251
           FFAY +N+D ISLDYALF+EN G +D+G+G +Y SLFDAQIDAV+AA+SA+ +  +K++V
Sbjct: 202 FFAYAANADKISLDYALFKENAGNIDSGTGLKYNSLFDAQIDAVYAALSAVGFKGVKVMV 261

Query: 252 TETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGTPLRPKADLTVYLFALFNENKKN 311
           TETGWPS GDENEIGAS  NAAAYN  LV+R+L+G GTPLRP   L VYLFALFNEN+K 
Sbjct: 262 TETGWPSVGDENEIGASESNAAAYNAGLVKRVLTGKGTPLRPTEPLNVYLFALFNENQKP 321

Query: 312 GPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDKPSPSPSPATGGDGKSGPVHGDGNVSK 371
           GPTSERNYGLFYPNE  VY++PFT +           S +P  G  GK         V  
Sbjct: 322 GPTSERNYGLFYPNEGKVYNVPFTKK-----------STTPVNGNRGK---------VPV 381

Query: 372 SQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQVGATCYNPNTLEAHASYAFNSY 431
           +  G+TWCV++GE  KEKLQ +LDYACGEGGADCRPIQ GATCY+P +LEAHASYAFNSY
Sbjct: 382 THEGHTWCVSNGEVAKEKLQEALDYACGEGGADCRPIQPGATCYHPESLEAHASYAFNSY 441

Query: 432 YQKNGRKTGTCYFGGAAYVVTQPPKY 458
           YQKN R+ GTC+FGGAA+VVTQPP+Y
Sbjct: 442 YQKNSRRVGTCFFGGAAHVVTQPPRY 446

[Close]

BLAST of Carg04638-RA vs. TAIR 10
Match: AT5G55180.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 580.5 bits (1495), Expect = 1.2e-165
Identity = 293/448 (65.40%), Postives = 347/448 (77.46%), Query Frame = 0


Query: 12  AGSIGVNYGRIANNLPSALKVVNLLKSRGLQRVKVYDTDPAVLRAISGSGIKVTVDLPNE 71
           +G IGVNYGRIA+NLP+  KVV LLK++G+ R+K+YDT+  VL A++ SGIKV V LPNE
Sbjct: 24  SGMIGVNYGRIADNLPAPEKVVELLKTQGINRIKLYDTETTVLTALANSGIKVVVSLPNE 83

Query: 72  LLFAAAKRLTFAYAWVEKNIVAYYPSTEIEAIAVGNEVFVDPHNTTSFLIPAMKNIHQAL 131
            L +AA   ++   WV+ NI  Y P+T+IEAIAVGNEVFVDP NTT++L+PAMKN+  +L
Sbjct: 84  NLASAAADQSYTDTWVQDNIKKYIPATDIEAIAVGNEVFVDPRNTTTYLVPAMKNVQSSL 143

Query: 132 VKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPELIESVFRPMLEFLRQTGSYLMVNAYP 191
           VK+NL  SIK+SSPIALSAL +SYP SAGSF+PELIE V +PML+ LR+T S+LMVNAYP
Sbjct: 144 VKFNLDKSIKISSPIALSALASSYPPSAGSFKPELIEPVIKPMLDLLRKTSSHLMVNAYP 203

Query: 192 FFAYESNSDVISLDYALFRENPGVVDAGSGYRYFSLFDAQIDAVFAAMSALKYDDIKMVV 251
           FFAY +N+D ISLDYALF+EN G VD+G+G +Y SL DAQIDAVFAAMSA+ ++D+K+VV
Sbjct: 204 FFAYAANADKISLDYALFKENAGNVDSGNGLKYNSLLDAQIDAVFAAMSAVGFNDVKLVV 263

Query: 252 TETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGTPLRPKADLTVYLFALFNENKKN 311
           TETGWPS GDENEIGA   NAAAYNG LV+R+L+G GTPL+PK  L VYLFALFNEN+K 
Sbjct: 264 TETGWPSAGDENEIGAGSANAAAYNGGLVKRVLTGNGTPLKPKEPLNVYLFALFNENQKT 323

Query: 312 GPTSERNYGLFYPNEESVYDIPFTTEGLKDYHDKPSPSPSPATGGDGKSGPVHGDGN--- 371
           GPTSERNYGLFYPNE  VYD+                        +GKS PV+ +     
Sbjct: 324 GPTSERNYGLFYPNENKVYDVSL----------------------NGKSTPVNDNKEKVV 383

Query: 372 -VSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQVGATCYNPNTLEAHASYA 431
            V  S  G TWCVA+G+  KEKLQ  LDYACGEGGADCRPIQ GATCYNP +LEAHASYA
Sbjct: 384 PVKPSLVGQTWCVANGKTTKEKLQEGLDYACGEGGADCRPIQPGATCYNPESLEAHASYA 443

Query: 432 FNSYYQKNGRKTGTCYFGGAAYVVTQPP 456
           FNSYYQKN R  GTC FGGAAYVV+QPP
Sbjct: 444 FNSYYQKNARGVGTCNFGGAAYVVSQPP 449

[Close]

BLAST of Carg04638-RA vs. TAIR 10
Match: AT4G29360.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 364.4 bits (934), Expect = 1.4e-100
Identity = 191/457 (41.79%), Postives = 282/457 (61.71%), Query Frame = 0


Query: 12  AGSIGVNYGRIANNLPSALKVVNLLKSRGLQRVKVYDTDPAVLRAISGSGIKVTVDLPNE 71
           A  IG+ YGR A+NLPS  +V  L++   ++ V++YD +  VL+A + +GI++ + +PN 
Sbjct: 24  ASKIGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANIDVLKAFANTGIELMIGVPNA 83

Query: 72  LLFAAAKRLTFAYAWVEKNIVAYYPSTEIEAIAVGNEVFVDPHNTTSFLIPAMKNIHQAL 131
            L A A+  +    W+  NI+ YYPST+I +I+VG EV   P N T  ++PAM+NIH AL
Sbjct: 84  DLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPDNATGLVLPAMRNIHTAL 143

Query: 132 VKYNLHSSIKVSSPIALSALQNSYPSSAGSFRPELIESVFRPMLEFLRQTGSYLMVNAYP 191
            K  L   IK+SS  +L+ L  S+P S+ SF  +   +  +PMLEFL +  S  M++ YP
Sbjct: 144 KKSGLDKKIKISSSHSLAILSRSFPPSSASFSKK-HSAFLKPMLEFLVENESPFMIDLYP 203

Query: 192 FFAYESNSDVISLDYALFRENPGVVDAGSGYRYFSLFDAQIDAVFAAMSALKYDDIKMVV 251
           ++AY  +++ + L+YALF  +  VVD  +G  Y ++FDAQ+DA++ A++A+ +  +K++V
Sbjct: 204 YYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLDAIYFALTAMSFKTVKVMV 263

Query: 252 TETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGTPLRPKADLTVYLFALFNENKKN 311
           TE+GWPSKG   E  A+ ENA AYN NL+R ++   GTP +P  ++ VYLF+LFNEN+K 
Sbjct: 264 TESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPGEEIDVYLFSLFNENRKP 323

Query: 312 GPTSERNYGLFYPNEESVYDIPFTTEGLKDYH-------DKPSPSPSPATGGDGKSGPVH 371
           G  SERN+G+FY N  +VY + FT E               PSPS SP   G+       
Sbjct: 324 GIESERNWGMFYANGTNVYALDFTGENTTPVSPTNSTTGTSPSPSSSPIINGNSTVTIGG 383

Query: 372 GDGNVSKSQTGNTWCVASGEAGKEKLQASLDYACGEGGADCRPIQVGATCYNPNTLEAHA 431
           G G  +K      WC+AS +A   +LQ +LD+ACG G  DC  +Q    C+ P+T+ +HA
Sbjct: 384 GGGGGTKK-----WCIASSQASVTELQTALDWACGPGNVDCSAVQPDQPCFEPDTVLSHA 443

Query: 432 SYAFNSYYQKNGRKTGTCYFGGAAYVVTQPPKYERIL 462
           SYAFN+YYQ++G  +  C F GA+  V + P Y   L
Sbjct: 444 SYAFNTYYQQSGASSIDCSFNGASVEVDKDPSYGNCL 474

The following BLAST results are available for this feature:

BLAST of Carg04638-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7022968.1	6.9e-264	100.00	Glucan endo-1,3-beta-glucosidase 13, partial [Cucurbita argyrosperma subsp. argy...	[more]
KAG6589270.1	4.8e-257	96.39	Glucan endo-1,3-beta-glucosidase 12, partial [Cucurbita argyrosperma subsp. soro...	[more]
XP_022930867.1	1.7e-254	95.54	glucan endo-1,3-beta-glucosidase 12-like [Cucurbita moschata]	[more]
XP_023531393.1	4.2e-253	94.92	glucan endo-1,3-beta-glucosidase 12-like [Cucurbita pepo subsp. pepo]	[more]
XP_022989090.1	1.4e-251	94.48	glucan endo-1,3-beta-glucosidase 12-like [Cucurbita maxima]	[more]

BLAST of Carg04638-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VYE5	1.9e-99	41.79	Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360...	[more]
Q9FJU9	6.8e-97	40.49	Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590...	[more]
Q9ZU91	9.5e-91	40.27	Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 ...	[more]
Q9C7U5	4.7e-90	38.89	Glucan endo-1,3-beta-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66250 ...	[more]
Q94CD8	2.4e-89	39.22	Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 ...	[more]

BLAST of Carg04638-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1ERV0	8.3e-255	95.54	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111437224 P...	[more]
A0A6J1JLE7	6.6e-252	94.48	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111486266 PE=...	[more]
A0A6J1I0V3	4.1e-238	89.48	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111468418 PE=...	[more]
A0A6J1GMW6	6.0e-237	89.06	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111455410 P...	[more]
A0A0A0KEL0	1.9e-235	90.33	(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_6G421570 PE=3...	[more]

BLAST of Carg04638-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G05790.1	6.1e-202	75.83	O-Glycosyl hydrolases family 17 protein	[more]
AT5G55180.1	4.9e-167	65.56	O-Glycosyl hydrolases family 17 protein	[more]
AT4G26830.1	3.2e-166	65.70	O-Glycosyl hydrolases family 17 protein	[more]
AT5G55180.2	1.2e-165	65.40	O-Glycosyl hydrolases family 17 protein	[more]
AT4G29360.1	1.4e-100	41.79	O-Glycosyl hydrolases family 17 protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 377..461 e-value: 3.0E-43 score: 159.7
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 377..448 e-value: 1.3E-22 score: 80.2
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 15..334 e-value: 2.3E-90 score: 303.4
IPR000490	Glycoside hydrolase family 17	PROSITE	PS00587	GLYCOSYL_HYDROL_F17	coord: 247..260
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 13..343 e-value: 8.9E-126 score: 421.2
None	No IPR available	GENE3D	1.20.58.1040		coord: 371..459 e-value: 3.6E-11 score: 45.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 337..373
None	No IPR available	PANTHER	PTHR32227:SF350	O-GLYCOSYL HYDROLASES FAMILY 17 PROTEIN-RELATED	coord: 10..458
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 10..458
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 15..333

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg04638	Carg04638	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg04638-RA:1	Carg04638-RA:1	exon
Carg04638-RA:2	Carg04638-RA:2	exon
Carg04638-RA:3	Carg04638-RA:3	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg04638-RA:cds	Carg04638-RA:cds	CDS
Carg04638-RA:cds	Carg04638-RA:cds_2	CDS
Carg04638-RA:cds	Carg04638-RA:cds_3	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg04638-RA	Carg04638-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0046658	anchored component of plasma membrane
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity