Carg04401-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg04401-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase
Location	Carg_Chr04: 20603125 .. 20606299 (+)
Sequence length	555
RNA-Seq Expression	Carg04401-RA
Synteny	Carg04401-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGTGGAACTGGAAGAGAATGATGTTCAGGAAAAGAAGATTAGGATTGGAGTTTGCGTGATGGTAAAGAAGGTGAAATGCGACGCCAAGCTTAATCATGTCCGCATCTCACTCTGAAACAGAACTCTATTTTGTTTGCTTATCATCTTACTCTGTGAACTTCTGCACTGCATAGGTGTCCTCGTCGCCAATGAGACAGATATTAGATAGATTGGAGGCCTTTGGTGAATTTGAGGTACGTTGTTTTTTAATCATCCAACCGTGTTTCTTATATTTTCGAAGTTCAAATCTCCTATAGTTGCTACAAGTTTCATTGCACGCGCTAGTTTTTCCCTCCCTGTTATTAATGAATGACAGATGGACTATGAAGACCTAGTTGTTTAAGAAATGAAGTAGTGTTATAATTTCTCCTTGGCAGTGTGGTTATATCTCATATCTTGTAGAGGTTTGCGAAATTAAGCTTTCACTGGTGTATATTTGCGGAGATTTAATTTTTCTGATTGTATTATAAGTTTATGCTTTTGCGGATGTTTATTTCTCATTGGTCTTTTTCATATGTGGACTGATTGTAGATAATAGTATTTGGAGACAAGGTCATTCTAGAAGATCCAATAGAGAGGTATAGTATATGGTTACAACCAGAATATAACTATTTTGAGGTCGCGCATTCAATTTTTTTCATATTTGGTTTTGTATTATCGCATGTATTTCATGAGAAAGGTTAGCTGCTATTTTGACCCTCTCCTTTCTTATAACCATGAGCAGACTCTTCATCACTTGGTACATTGAGAAGTTTTACATTCTCTTGCCAATATTTAACCCGCCACGTGCTAAATAACTTGTTCTACTCTGGCCAGTGAATATCACATCTTTGGCTATGGATTTACTGTTCAAATTGTGATGCTATCTTGAACGAACTTCAGACAAGTAGCCTTTTAGAAGGGAAAAGATGGGCTACCGAAGTTTTAGCTACTTCTTGGGGAGTTTGGTTTGAAAGGAAGAGATGTTTTGATTTTTTAAAAGAATAAGTTAGTGGCATACAGATAGTTGGGAATATATTCACTTAAATATTACTTTACGGGCTTTTTGATTACAAAAGAGAGAACATATATCTTTTAACTTGCGGTTTTTTTAATTACAAAAGAGAACATAAATCTTTTCGCATTATTTAAGGGGTGGAGATCTTACTAATTTGAAGGTCTCTTCTCTTTCCTTGCCTTCCATTTTGGGGATAGTTTTAATCATTTTAATTTAGCACGTGTATATTCACATATTTCTTTCTTTTCTTTTTTCCAAATTATTTCTACTAAACATTGGATAGATTATTCGTTTCTACTAAATAAACATTGGATATATGACATGGAGGTGAAATTTTAGAGTTTGAGGTCTTAGACATAATAGGCATAATAACTCAGATAGTGTGCATGTGTGTTGCTGGTGAATCAAGCTTACTGTACTTGATGAATTATTAGATTCTTCTTGTCTATACGTTTAACTATCAGAATTCTTGTTTCAGATGGCCAGATTGTGATTGCTTGATTGCCTTTTACTCCAGTGGCTATCCATTGGAGAAGGTTGAAGAGTATGCTGCTCTTCGCAAGTAAGCTTTACTTTTTACTCATTTATTAAGTACATTGTGTTCATCAATAAATTATTTATCAGTGCTTCATCCATGCTAGTTCTTGCGAGTATTGACAAAGTCTTCCTAGGCCTTTTACTGCCGAAGCAGACATCTCCTTTTCCAGAATAATTTAGAAAAGATTGGACCTATCGTTTGAAATTTTTTGGTTGTATCAATTCCTCTTCTCCCCTTTGAAGCTAATTGTAAAAATTTAGTCTCGATGTAACTACTTTCATTTTCCTCTTAGAATTCCACACTGTAAAAATTGAGTTAGCTTCAAGGAGTATGCAAGTTTGGCAGTCATTGACATCATCCACAAACTTTTGTTTGCGTGGATGTGGGAATGTCTCTCCTAATGTTTTATATATTCATATGAGCCTAAAGTTTTCGTTCTTTGACATCTAGAAGGTCGATTACTTCCTTATAATGCCTTTACATCAATGGAATATTCAATAACATACTCTCATATTCAGACCCTTTTTGGTAAATGAGTTGGAACCACAATACCTTCTTCATGACCGAAGGAAGGTTTACCAGGTATGCCTCTTTGATTTGTATCATTTTATGCTATTTCTTTTACATCTGTTAAATACGGCAGTTTTTATGTGGTGCTTTGTCTTAAGTTATTTAGTATGTTGTTGGTCATATAATCCGTTAATCTTTCTATTTGACTTTCACAGAGTTACTGATTTTACATGCTTAACCTTTACTGATTTAAGGAGCGTGAATGTTTCTTCTTCAATAAAAATTATTCATTAAGATGGGAGAAAATGCAGAGGAAAGGAAGTATCAGGAATATCCTCTCCCCTAAAGCCAACTGAAAAGGTGGTTTTATAGAAGTCCCCATTGCAAAACACTTTAAATGATTGAAGTGCCTAAAATCTTAATTCACTATTTTATGGAAGAAAGAAAAACTACTGTTAACTATTGAAGGATTCTCCTTAAGTTAGGAGATATTTGTGTAATTCATGAGTTAGTATACAAGTTCAGAATAAATGGTATCTATTCTGTTACTCAGCTTGAGCTTGAGGGGAGTGTCAAAAGATATTTGTATAATCCCTTTAAATAAGCGAAGTAGTTGAAATTAAGATTTATTTAGCCTGTCTCCCTTTTATAAAAGGGACATGCAATTCTCGAAAAATTAGTAAGAAAACAGCAAGCTAATTTAGACTTTGCAGCATGGTATCAAAGCATAGCTGTCATCAAAATCCTCATTCAACAAGACCCTCAGACTTTGCTGCTCAAAACAAAAGAAGTTGCTAAAAATTTTCAGCCTCTACAAGGAGATTGCAAAATGGAAAAAAAAAAGGGGGGGGAAAAAGAAAACCGCAAATTGATTTTTTTTTTTCCCAGCAATCTGGAAATAATATTAAGTCCAAATATCCCCGACTTCCTCTGCTGCTCAAATCCCCAGCAATCTGCAAATGTGTTCCACCAATGGACCAAAATTATTCTCTCTGTTCCACAAGCCGCAAACCCACCAAGAAACATATCCATTCTAGCCTCCATGATCAGAGTCTTACTTCTTTCATCGTGAGCCTTCATAGCTGA

mRNA sequence

ATGGAGGTGGAACTGGAAGAGAATGATGTTCAGGAAAAGAAGATTAGGATTGGAGTTTGCGTGATGGTAAAGAAGGTGAAATGCGACGCCAAGCTTAATCATGTGTCCTCGTCGCCAATGAGACAGATATTAGATAGATTGGAGGCCTTTGGTGAATTTGAGATAATAGTATTTGGAGACAAGGTCATTCTAGAAGATCCAATAGAGAGATGGCCAGATTGTGATTGCTTGATTGCCTTTTACTCCAGTGGCTATCCATTGGAGAAGGTTGAAGAGTATGCTGCTCTTCGCAAACCCTTTTTGGTAAATGAGTTGGAACCACAATACCTTCTTCATGACCGAAGGAAGGTTTACCAGCAATCTGGAAATAATATTAAGTCCAAATATCCCCGACTTCCTCTGCTGCTCAAATCCCCAGCAATCTGCAAATGTGTTCCACCAATGGACCAAAATTATTCTCTCTGTTCCACAAGCCGCAAACCCACCAAGAAACATATCCATTCTAGCCTCCATGATCAGAGTCTTACTTCTTTCATCGTGAGCCTTCATAGCTGA

Coding sequence (CDS)

ATGGAGGTGGAACTGGAAGAGAATGATGTTCAGGAAAAGAAGATTAGGATTGGAGTTTGCGTGATGGTAAAGAAGGTGAAATGCGACGCCAAGCTTAATCATGTGTCCTCGTCGCCAATGAGACAGATATTAGATAGATTGGAGGCCTTTGGTGAATTTGAGATAATAGTATTTGGAGACAAGGTCATTCTAGAAGATCCAATAGAGAGATGGCCAGATTGTGATTGCTTGATTGCCTTTTACTCCAGTGGCTATCCATTGGAGAAGGTTGAAGAGTATGCTGCTCTTCGCAAACCCTTTTTGGTAAATGAGTTGGAACCACAATACCTTCTTCATGACCGAAGGAAGGTTTACCAGCAATCTGGAAATAATATTAAGTCCAAATATCCCCGACTTCCTCTGCTGCTCAAATCCCCAGCAATCTGCAAATGTGTTCCACCAATGGACCAAAATTATTCTCTCTGTTCCACAAGCCGCAAACCCACCAAGAAACATATCCATTCTAGCCTCCATGATCAGAGTCTTACTTCTTTCATCGTGAGCCTTCATAGCTGA

Protein sequence

MEVELEENDVQEKKIRIGVCVMVKKVKCDAKLNHVSSSPMRQILDRLEAFGEFEIIVFGDKVILEDPIERWPDCDCLIAFYSSGYPLEKVEEYAALRKPFLVNELEPQYLLHDRRKVYQQSGNNIKSKYPRLPLLLKSPAICKCVPPMDQNYSLCSTSRKPTKKHIHSSLHDQSLTSFIVSLHS

Homology

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BLAST of Carg04401-RA vs. NCBI nr
Match: KAG7033152.1 (Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase VIP2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 375.2 bits (962), Expect = 3.3e-100
Identity = 184/184 (100.00%), Postives = 184/184 (100.00%), Query Frame = 0


Query: 1   MEVELEENDVQEKKIRIGVCVMVKKVKCDAKLNHVSSSPMRQILDRLEAFGEFEIIVFGD 60
           MEVELEENDVQEKKIRIGVCVMVKKVKCDAKLNHVSSSPMRQILDRLEAFGEFEIIVFGD
Sbjct: 1   MEVELEENDVQEKKIRIGVCVMVKKVKCDAKLNHVSSSPMRQILDRLEAFGEFEIIVFGD 60

Query: 61  KVILEDPIERWPDCDCLIAFYSSGYPLEKVEEYAALRKPFLVNELEPQYLLHDRRKVYQQ 120
           KVILEDPIERWPDCDCLIAFYSSGYPLEKVEEYAALRKPFLVNELEPQYLLHDRRKVYQQ
Sbjct: 61  KVILEDPIERWPDCDCLIAFYSSGYPLEKVEEYAALRKPFLVNELEPQYLLHDRRKVYQQ 120

Query: 121 SGNNIKSKYPRLPLLLKSPAICKCVPPMDQNYSLCSTSRKPTKKHIHSSLHDQSLTSFIV 180
           SGNNIKSKYPRLPLLLKSPAICKCVPPMDQNYSLCSTSRKPTKKHIHSSLHDQSLTSFIV
Sbjct: 121 SGNNIKSKYPRLPLLLKSPAICKCVPPMDQNYSLCSTSRKPTKKHIHSSLHDQSLTSFIV 180

Query: 181 SLHS 185
           SLHS
Sbjct: 181 SLHS 184

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BLAST of Carg04401-RA vs. NCBI nr
Match: XP_022953054.1 (inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase VIP2-like isoform X1 [Cucurbita moschata] >XP_022990036.1 inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase VIP2-like isoform X1 [Cucurbita maxima] >XP_023525946.1 inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase VIP2-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 245.0 bits (624), Expect = 5.2e-61
Identity = 119/120 (99.17%), Postives = 120/120 (100.00%), Query Frame = 0


Query: 1   MEVELEENDVQEKKIRIGVCVMVKKVKCDAKLNHVSSSPMRQILDRLEAFGEFEIIVFGD 60
           MEVELEENDVQEKKIRIGVCVMVKKVKCDAKLNHVSSSPMRQILDRLEAFGEFEIIVFGD
Sbjct: 4   MEVELEENDVQEKKIRIGVCVMVKKVKCDAKLNHVSSSPMRQILDRLEAFGEFEIIVFGD 63

Query: 61  KVILEDPIERWPDCDCLIAFYSSGYPLEKVEEYAALRKPFLVNELEPQYLLHDRRKVYQQ 120
           KVILEDPIERWPDCDCLIAFYSSGYPLEKVEEYAALRKPFLVNELEPQYLLHDRRKVYQ+
Sbjct: 64  KVILEDPIERWPDCDCLIAFYSSGYPLEKVEEYAALRKPFLVNELEPQYLLHDRRKVYQR 123

[Close]

BLAST of Carg04401-RA vs. NCBI nr
Match: XP_038890407.1 (inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase VIP2-like isoform X1 [Benincasa hispida] >XP_038890408.1 inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase VIP2-like isoform X1 [Benincasa hispida])

HSP 1 Score: 234.6 bits (597), Expect = 7.0e-58
Identity = 112/119 (94.12%), Postives = 118/119 (99.16%), Query Frame = 0


Query: 2   EVELEENDVQEKKIRIGVCVMVKKVKCDAKLNHVSSSPMRQILDRLEAFGEFEIIVFGDK 61
           ++E+EENDV EKKIRIGVCVMVKKVKCDAKLNHVSSSPMRQILDRLEAFGEFEIIVFGDK
Sbjct: 3   DMEVEENDVYEKKIRIGVCVMVKKVKCDAKLNHVSSSPMRQILDRLEAFGEFEIIVFGDK 62

Query: 62  VILEDPIERWPDCDCLIAFYSSGYPLEKVEEYAALRKPFLVNELEPQYLLHDRRKVYQQ 121
           VILEDPIERWPDCDCLIAFYSSGYPLEKVE+YAALRKPFLVNELEPQYLLHDRRKVY++
Sbjct: 63  VILEDPIERWPDCDCLIAFYSSGYPLEKVEKYAALRKPFLVNELEPQYLLHDRRKVYER 121

[Close]

BLAST of Carg04401-RA vs. NCBI nr
Match: XP_022134518.1 (inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase VIP2-like isoform X1 [Momordica charantia])

HSP 1 Score: 233.4 bits (594), Expect = 1.6e-57
Identity = 112/119 (94.12%), Postives = 117/119 (98.32%), Query Frame = 0


Query: 2   EVELEENDVQEKKIRIGVCVMVKKVKCDAKLNHVSSSPMRQILDRLEAFGEFEIIVFGDK 61
           ++E+EEN V EKKIRIGVCVMVKKVKCDAKLNHVSSSPMRQILDRLEAFGEFEIIVFGDK
Sbjct: 3   DMEVEENGVHEKKIRIGVCVMVKKVKCDAKLNHVSSSPMRQILDRLEAFGEFEIIVFGDK 62

Query: 62  VILEDPIERWPDCDCLIAFYSSGYPLEKVEEYAALRKPFLVNELEPQYLLHDRRKVYQQ 121
           VILEDPIERWPDCDCLIAFYSSGYPLEKVE+YAALRKPFLVNELEPQYLLHDRRKVYQ+
Sbjct: 63  VILEDPIERWPDCDCLIAFYSSGYPLEKVEKYAALRKPFLVNELEPQYLLHDRRKVYQR 121

[Close]

BLAST of Carg04401-RA vs. NCBI nr
Match: XP_008459290.1 (PREDICTED: inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 2-like isoform X1 [Cucumis melo])

HSP 1 Score: 232.3 bits (591), Expect = 3.5e-57
Identity = 110/119 (92.44%), Postives = 117/119 (98.32%), Query Frame = 0


Query: 2   EVELEENDVQEKKIRIGVCVMVKKVKCDAKLNHVSSSPMRQILDRLEAFGEFEIIVFGDK 61
           ++E+EEND  EKKIRIGVCVMVKKVKCDAKLNHVSSSPMRQILDRLEAFGEFEI+VFGDK
Sbjct: 3   DMEVEENDFYEKKIRIGVCVMVKKVKCDAKLNHVSSSPMRQILDRLEAFGEFEIVVFGDK 62

Query: 62  VILEDPIERWPDCDCLIAFYSSGYPLEKVEEYAALRKPFLVNELEPQYLLHDRRKVYQQ 121
           VILE+PIERWPDCDCLIAFYSSGYPLEKVE+YAALRKPFLVNELEPQYLLHDRRKVYQ+
Sbjct: 63  VILEEPIERWPDCDCLIAFYSSGYPLEKVEQYAALRKPFLVNELEPQYLLHDRRKVYQR 121

[Close]

BLAST of Carg04401-RA vs. ExPASy Swiss-Prot
Match: Q84WW3 (Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase VIP2 OS=Arabidopsis thaliana OX=3702 GN=VIP2 PE=1 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 4.0e-40
Identity = 82/115 (71.30%), Postives = 93/115 (80.87%), Query Frame = 0


Query: 5   LEENDVQEKKIRIGVCVMVKKVKCDAKLNHVSSSPMRQILDRLEAFGEFEIIVFGDKVIL 64
           +EE    +KKI IGVCVM KK         V S+PM QI+DR+ AFGEFEII FGDKVIL
Sbjct: 3   VEEGAGVDKKITIGVCVMEKK---------VFSAPMGQIMDRIHAFGEFEIIHFGDKVIL 62

Query: 65  EDPIERWPDCDCLIAFYSSGYPLEKVEEYAALRKPFLVNELEPQYLLHDRRKVYQ 120
           EDP+E WP CDCLIAFYSSGYPLEKV+ Y++LRKPFLVNEL+PQYLLHDRRKVY+
Sbjct: 63  EDPVESWPICDCLIAFYSSGYPLEKVQAYSSLRKPFLVNELDPQYLLHDRRKVYE 108

[Close]

BLAST of Carg04401-RA vs. ExPASy Swiss-Prot
Match: F4J8C6 (Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase VIP1 OS=Arabidopsis thaliana OX=3702 GN=VIP1 PE=1 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 3.4e-39
Identity = 84/119 (70.59%), Postives = 96/119 (80.67%), Query Frame = 0


Query: 1   MEVELEENDVQEKKIRIGVCVMVKKVKCDAKLNHVSSSPMRQILDRLEAFGEFEIIVFGD 60
           ME+E   + V E KI+IGVCVM KK         V S+PM +ILDRL++FGEFEI+ FGD
Sbjct: 1   MEMEEGASGVGE-KIKIGVCVMEKK---------VFSAPMGEILDRLQSFGEFEILHFGD 60

Query: 61  KVILEDPIERWPDCDCLIAFYSSGYPLEKVEEYAALRKPFLVNELEPQYLLHDRRKVYQ 120
           KVILEDPIE WP CDCLIAF+SSGYPLEK + YAALRKPFLVNEL+PQYLLHDRRKVY+
Sbjct: 61  KVILEDPIESWPICDCLIAFHSSGYPLEKAQAYAALRKPFLVNELDPQYLLHDRRKVYE 109

[Close]

BLAST of Carg04401-RA vs. ExPASy Swiss-Prot
Match: O43314 (Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 2 OS=Homo sapiens OX=9606 GN=PPIP5K2 PE=1 SV=3)

HSP 1 Score: 117.1 bits (292), Expect = 2.1e-25
Identity = 59/148 (39.86%), Postives = 89/148 (60.14%), Query Frame = 0


Query: 4   ELEENDVQEKKIRIGVCVMVKKVKCDAKLNHVSSSPMRQILDRLEAFGEFEIIVFGDKVI 63
           E E++   E++I +G+C M KK K         S PM++IL+R+  F    ++VF ++VI
Sbjct: 33  EEEDDSPPERQIVVGICSMAKKSK---------SKPMKEILERISLFKYITVVVFEEEVI 92

Query: 64  LEDPIERWPDCDCLIAFYSSGYPLEKVEEYAALRKPFLVNELEPQYLLHDRRKVYQ--QS 123
           L +P+E WP CDCLI+F+S G+PL+K   YA LR PF++N+L  QYL+ DRR+VY   Q+
Sbjct: 93  LNEPVENWPLCDCLISFHSKGFPLDKAVAYAKLRNPFVINDLNMQYLIQDRREVYSILQA 152

Query: 124 GNNIKSKYPRLPLLLKSPAICKCVPPMD 150
              +  +Y  L     +P  C  +   D
Sbjct: 153 EGILLPRYAILNRDPNNPKECNLIEGED 171

[Close]

BLAST of Carg04401-RA vs. ExPASy Swiss-Prot
Match: Q5REW0 (Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 2 OS=Pongo abelii OX=9601 GN=PPIP5K2 PE=2 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 2.8e-25
Identity = 59/148 (39.86%), Postives = 89/148 (60.14%), Query Frame = 0


Query: 4   ELEENDVQEKKIRIGVCVMVKKVKCDAKLNHVSSSPMRQILDRLEAFGEFEIIVFGDKVI 63
           E E++   E++I +G+C M KK K         S PM++IL+R+  F    ++VF ++VI
Sbjct: 33  EEEDDSPPERQIVVGICSMAKKSK---------SKPMKEILERVSLFKYITVVVFEEEVI 92

Query: 64  LEDPIERWPDCDCLIAFYSSGYPLEKVEEYAALRKPFLVNELEPQYLLHDRRKVYQ--QS 123
           L +P+E WP CDCLI+F+S G+PL+K   YA LR PF++N+L  QYL+ DRR+VY   Q+
Sbjct: 93  LNEPVENWPLCDCLISFHSKGFPLDKAVAYAKLRNPFVINDLNMQYLIQDRREVYSILQA 152

Query: 124 GNNIKSKYPRLPLLLKSPAICKCVPPMD 150
              +  +Y  L     +P  C  +   D
Sbjct: 153 EGILLPRYAILNRDPNNPKECNLIEGED 171

[Close]

BLAST of Carg04401-RA vs. ExPASy Swiss-Prot
Match: Q6ZQB6 (Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 2 OS=Mus musculus OX=10090 GN=Ppip5k2 PE=1 SV=3)

HSP 1 Score: 116.3 bits (290), Expect = 3.6e-25
Identity = 59/150 (39.33%), Postives = 88/150 (58.67%), Query Frame = 0


Query: 2   EVELEENDVQEKKIRIGVCVMVKKVKCDAKLNHVSSSPMRQILDRLEAFGEFEIIVFGDK 61
           E E E+    E++I +G+C M KK K         S PM++IL+R+  F    ++VF ++
Sbjct: 37  EEEEEDESPPERQIVVGICSMAKKSK---------SKPMKEILERISLFKYITVVVFEEE 96

Query: 62  VILEDPIERWPDCDCLIAFYSSGYPLEKVEEYAALRKPFLVNELEPQYLLHDRRKVYQ-- 121
           +IL +P+E WP CDCLI+F+S G+PL+K   YA LR PF++N+L  QYL+ DRR VY   
Sbjct: 97  IILNEPVENWPLCDCLISFHSKGFPLDKAVAYAKLRNPFVINDLNMQYLIQDRRDVYSIL 156

Query: 122 QSGNNIKSKYPRLPLLLKSPAICKCVPPMD 150
           Q+   +  +Y  L     +P  C  +   D
Sbjct: 157 QAEGILLPRYAILNRDPNNPKECNLIEGED 177

[Close]

BLAST of Carg04401-RA vs. ExPASy TrEMBL
Match: A0A6J1JLU3 (Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase OS=Cucurbita maxima OX=3661 GN=LOC111487048 PE=3 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 2.5e-61
Identity = 119/120 (99.17%), Postives = 120/120 (100.00%), Query Frame = 0


Query: 1   MEVELEENDVQEKKIRIGVCVMVKKVKCDAKLNHVSSSPMRQILDRLEAFGEFEIIVFGD 60
           MEVELEENDVQEKKIRIGVCVMVKKVKCDAKLNHVSSSPMRQILDRLEAFGEFEIIVFGD
Sbjct: 4   MEVELEENDVQEKKIRIGVCVMVKKVKCDAKLNHVSSSPMRQILDRLEAFGEFEIIVFGD 63

Query: 61  KVILEDPIERWPDCDCLIAFYSSGYPLEKVEEYAALRKPFLVNELEPQYLLHDRRKVYQQ 120
           KVILEDPIERWPDCDCLIAFYSSGYPLEKVEEYAALRKPFLVNELEPQYLLHDRRKVYQ+
Sbjct: 64  KVILEDPIERWPDCDCLIAFYSSGYPLEKVEEYAALRKPFLVNELEPQYLLHDRRKVYQR 123

[Close]

BLAST of Carg04401-RA vs. ExPASy TrEMBL
Match: A0A6J1GNJ6 (Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase OS=Cucurbita moschata OX=3662 GN=LOC111455571 PE=3 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 2.5e-61
Identity = 119/120 (99.17%), Postives = 120/120 (100.00%), Query Frame = 0


Query: 1   MEVELEENDVQEKKIRIGVCVMVKKVKCDAKLNHVSSSPMRQILDRLEAFGEFEIIVFGD 60
           MEVELEENDVQEKKIRIGVCVMVKKVKCDAKLNHVSSSPMRQILDRLEAFGEFEIIVFGD
Sbjct: 4   MEVELEENDVQEKKIRIGVCVMVKKVKCDAKLNHVSSSPMRQILDRLEAFGEFEIIVFGD 63

Query: 61  KVILEDPIERWPDCDCLIAFYSSGYPLEKVEEYAALRKPFLVNELEPQYLLHDRRKVYQQ 120
           KVILEDPIERWPDCDCLIAFYSSGYPLEKVEEYAALRKPFLVNELEPQYLLHDRRKVYQ+
Sbjct: 64  KVILEDPIERWPDCDCLIAFYSSGYPLEKVEEYAALRKPFLVNELEPQYLLHDRRKVYQR 123

[Close]

BLAST of Carg04401-RA vs. ExPASy TrEMBL
Match: A0A6J1C275 (Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase OS=Momordica charantia OX=3673 GN=LOC111006742 PE=3 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 7.5e-58
Identity = 112/119 (94.12%), Postives = 117/119 (98.32%), Query Frame = 0


Query: 2   EVELEENDVQEKKIRIGVCVMVKKVKCDAKLNHVSSSPMRQILDRLEAFGEFEIIVFGDK 61
           ++E+EEN V EKKIRIGVCVMVKKVKCDAKLNHVSSSPMRQILDRLEAFGEFEIIVFGDK
Sbjct: 3   DMEVEENGVHEKKIRIGVCVMVKKVKCDAKLNHVSSSPMRQILDRLEAFGEFEIIVFGDK 62

Query: 62  VILEDPIERWPDCDCLIAFYSSGYPLEKVEEYAALRKPFLVNELEPQYLLHDRRKVYQQ 121
           VILEDPIERWPDCDCLIAFYSSGYPLEKVE+YAALRKPFLVNELEPQYLLHDRRKVYQ+
Sbjct: 63  VILEDPIERWPDCDCLIAFYSSGYPLEKVEKYAALRKPFLVNELEPQYLLHDRRKVYQR 121

[Close]

BLAST of Carg04401-RA vs. ExPASy TrEMBL
Match: A0A1S3C9X6 (Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase OS=Cucumis melo OX=3656 GN=LOC103498465 PE=3 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 1.7e-57
Identity = 110/119 (92.44%), Postives = 117/119 (98.32%), Query Frame = 0


Query: 2   EVELEENDVQEKKIRIGVCVMVKKVKCDAKLNHVSSSPMRQILDRLEAFGEFEIIVFGDK 61
           ++E+EEND  EKKIRIGVCVMVKKVKCDAKLNHVSSSPMRQILDRLEAFGEFEI+VFGDK
Sbjct: 3   DMEVEENDFYEKKIRIGVCVMVKKVKCDAKLNHVSSSPMRQILDRLEAFGEFEIVVFGDK 62

Query: 62  VILEDPIERWPDCDCLIAFYSSGYPLEKVEEYAALRKPFLVNELEPQYLLHDRRKVYQQ 121
           VILE+PIERWPDCDCLIAFYSSGYPLEKVE+YAALRKPFLVNELEPQYLLHDRRKVYQ+
Sbjct: 63  VILEEPIERWPDCDCLIAFYSSGYPLEKVEQYAALRKPFLVNELEPQYLLHDRRKVYQR 121

[Close]

BLAST of Carg04401-RA vs. ExPASy TrEMBL
Match: A0A6J1JHH5 (Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase OS=Cucurbita maxima OX=3661 GN=LOC111487048 PE=3 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 4.3e-53
Identity = 110/120 (91.67%), Postives = 111/120 (92.50%), Query Frame = 0


Query: 1   MEVELEENDVQEKKIRIGVCVMVKKVKCDAKLNHVSSSPMRQILDRLEAFGEFEIIVFGD 60
           MEVELEENDVQEKKIRIGVCVMVKK         VSSSPMRQILDRLEAFGEFEIIVFGD
Sbjct: 4   MEVELEENDVQEKKIRIGVCVMVKK---------VSSSPMRQILDRLEAFGEFEIIVFGD 63

Query: 61  KVILEDPIERWPDCDCLIAFYSSGYPLEKVEEYAALRKPFLVNELEPQYLLHDRRKVYQQ 120
           KVILEDPIERWPDCDCLIAFYSSGYPLEKVEEYAALRKPFLVNELEPQYLLHDRRKVYQ+
Sbjct: 64  KVILEDPIERWPDCDCLIAFYSSGYPLEKVEEYAALRKPFLVNELEPQYLLHDRRKVYQR 114

[Close]

BLAST of Carg04401-RA vs. TAIR 10
Match: AT3G01310.1 (Phosphoglycerate mutase-like family protein )

HSP 1 Score: 172.6 bits (436), Expect = 3.0e-43
Identity = 87/119 (73.11%), Postives = 101/119 (84.87%), Query Frame = 0


Query: 1   MEVELEENDVQEKKIRIGVCVMVKKVKCDAKLNHVSSSPMRQILDRLEAFGEFEIIVFGD 60
           ME+E   + V E KI+IGVCVM KKVKC ++   V S+PM +ILDRL++FGEFEI+ FGD
Sbjct: 1   MEMEEGASGVGE-KIKIGVCVMEKKVKCGSE---VFSAPMGEILDRLQSFGEFEILHFGD 60

Query: 61  KVILEDPIERWPDCDCLIAFYSSGYPLEKVEEYAALRKPFLVNELEPQYLLHDRRKVYQ 120
           KVILEDPIE WP CDCLIAF+SSGYPLEK + YAALRKPFLVNEL+PQYLLHDRRKVY+
Sbjct: 61  KVILEDPIESWPICDCLIAFHSSGYPLEKAQAYAALRKPFLVNELDPQYLLHDRRKVYE 115

[Close]

BLAST of Carg04401-RA vs. TAIR 10
Match: AT5G15070.1 (Phosphoglycerate mutase-like family protein )

HSP 1 Score: 166.0 bits (419), Expect = 2.8e-41
Identity = 82/115 (71.30%), Postives = 93/115 (80.87%), Query Frame = 0


Query: 5   LEENDVQEKKIRIGVCVMVKKVKCDAKLNHVSSSPMRQILDRLEAFGEFEIIVFGDKVIL 64
           +EE    +KKI IGVCVM KK         V S+PM QI+DR+ AFGEFEII FGDKVIL
Sbjct: 3   VEEGAGVDKKITIGVCVMEKK---------VFSAPMGQIMDRIHAFGEFEIIHFGDKVIL 62

Query: 65  EDPIERWPDCDCLIAFYSSGYPLEKVEEYAALRKPFLVNELEPQYLLHDRRKVYQ 120
           EDP+E WP CDCLIAFYSSGYPLEKV+ Y++LRKPFLVNEL+PQYLLHDRRKVY+
Sbjct: 63  EDPVESWPICDCLIAFYSSGYPLEKVQAYSSLRKPFLVNELDPQYLLHDRRKVYE 108

[Close]

BLAST of Carg04401-RA vs. TAIR 10
Match: AT5G15070.2 (Phosphoglycerate mutase-like family protein )

HSP 1 Score: 166.0 bits (419), Expect = 2.8e-41
Identity = 82/115 (71.30%), Postives = 93/115 (80.87%), Query Frame = 0


Query: 5   LEENDVQEKKIRIGVCVMVKKVKCDAKLNHVSSSPMRQILDRLEAFGEFEIIVFGDKVIL 64
           +EE    +KKI IGVCVM KK         V S+PM QI+DR+ AFGEFEII FGDKVIL
Sbjct: 3   VEEGAGVDKKITIGVCVMEKK---------VFSAPMGQIMDRIHAFGEFEIIHFGDKVIL 62

Query: 65  EDPIERWPDCDCLIAFYSSGYPLEKVEEYAALRKPFLVNELEPQYLLHDRRKVYQ 120
           EDP+E WP CDCLIAFYSSGYPLEKV+ Y++LRKPFLVNEL+PQYLLHDRRKVY+
Sbjct: 63  EDPVESWPICDCLIAFYSSGYPLEKVQAYSSLRKPFLVNELDPQYLLHDRRKVYE 108

[Close]

BLAST of Carg04401-RA vs. TAIR 10
Match: AT3G01310.2 (Phosphoglycerate mutase-like family protein )

HSP 1 Score: 162.9 bits (411), Expect = 2.4e-40
Identity = 84/119 (70.59%), Postives = 96/119 (80.67%), Query Frame = 0


Query: 1   MEVELEENDVQEKKIRIGVCVMVKKVKCDAKLNHVSSSPMRQILDRLEAFGEFEIIVFGD 60
           ME+E   + V E KI+IGVCVM KK         V S+PM +ILDRL++FGEFEI+ FGD
Sbjct: 1   MEMEEGASGVGE-KIKIGVCVMEKK---------VFSAPMGEILDRLQSFGEFEILHFGD 60

Query: 61  KVILEDPIERWPDCDCLIAFYSSGYPLEKVEEYAALRKPFLVNELEPQYLLHDRRKVYQ 120
           KVILEDPIE WP CDCLIAF+SSGYPLEK + YAALRKPFLVNEL+PQYLLHDRRKVY+
Sbjct: 61  KVILEDPIESWPICDCLIAFHSSGYPLEKAQAYAALRKPFLVNELDPQYLLHDRRKVYE 109

The following BLAST results are available for this feature:

BLAST of Carg04401-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7033152.1	3.3e-100	100.00	Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase VIP2, p...	[more]
XP_022953054.1	5.2e-61	99.17	inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase VIP2-li...	[more]
XP_038890407.1	7.0e-58	94.12	inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase VIP2-li...	[more]
XP_022134518.1	1.6e-57	94.12	inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase VIP2-li...	[more]
XP_008459290.1	3.5e-57	92.44	PREDICTED: inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kin...	[more]

BLAST of Carg04401-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84WW3	4.0e-40	71.30	Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase VIP2 OS...	[more]
F4J8C6	3.4e-39	70.59	Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase VIP1 OS...	[more]
O43314	2.1e-25	39.86	Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 2 OS=Ho...	[more]
Q5REW0	2.8e-25	39.86	Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 2 OS=Po...	[more]
Q6ZQB6	3.6e-25	39.33	Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 2 OS=Mu...	[more]

BLAST of Carg04401-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JLU3	2.5e-61	99.17	Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase OS=Cucu...	[more]
A0A6J1GNJ6	2.5e-61	99.17	Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase OS=Cucu...	[more]
A0A6J1C275	7.5e-58	94.12	Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase OS=Momo...	[more]
A0A1S3C9X6	1.7e-57	92.44	Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase OS=Cucu...	[more]
A0A6J1JHH5	4.3e-53	91.67	Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase OS=Cucu...	[more]

BLAST of Carg04401-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G01310.1	3.0e-43	73.11	Phosphoglycerate mutase-like family protein	[more]
AT5G15070.1	2.8e-41	71.30	Phosphoglycerate mutase-like family protein	[more]
AT5G15070.2	2.8e-41	71.30	Phosphoglycerate mutase-like family protein	[more]
AT3G01310.2	2.4e-40	70.59	Phosphoglycerate mutase-like family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR040557	VIP1, N-terminal	PFAM	PF18086	PPIP5K2_N	coord: 15..113 e-value: 1.0E-36 score: 125.0
None	No IPR available	GENE3D	3.40.50.11950		coord: 8..133 e-value: 2.1E-45 score: 155.6
None	No IPR available	PANTHER	PTHR12750:SF15	INOSITOL HEXAKISPHOSPHATE AND DIPHOSPHOINOSITOL-PENTAKISPHOSPHATE KINASE	coord: 11..120
IPR037446	Histidine acid phosphatase, VIP1 family	PANTHER	PTHR12750	DIPHOSPHOINOSITOL PENTAKISPHOSPHATE KINASE	coord: 11..120

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg04401	Carg04401	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg04401-RA:6	Carg04401-RA:6	exon
Carg04401-RA:5	Carg04401-RA:5	exon
Carg04401-RA:4	Carg04401-RA:4	exon
Carg04401-RA:3	Carg04401-RA:3	exon
Carg04401-RA:2	Carg04401-RA:2	exon
Carg04401-RA:1	Carg04401-RA:1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg04401-RA:cds	Carg04401-RA:cds	CDS
Carg04401-RA:cds	Carg04401-RA:cds_2	CDS
Carg04401-RA:cds	Carg04401-RA:cds_3	CDS
Carg04401-RA:cds	Carg04401-RA:cds_4	CDS
Carg04401-RA:cds	Carg04401-RA:cds_5	CDS
Carg04401-RA:cds	Carg04401-RA:cds_6	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg04401-RA	Carg04401-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
cellular_component	GO:0005829	cytosol
molecular_function	GO:0000829	inositol heptakisphosphate kinase activity
molecular_function	GO:0102092	5-diphosphoinositol pentakisphosphate 3-kinase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0033857	diphosphoinositol-pentakisphosphate kinase activity
molecular_function	GO:0000827	inositol-1,3,4,5,6-pentakisphosphate kinase activity
molecular_function	GO:0052723	inositol hexakisphosphate 1-kinase activity
molecular_function	GO:0052724	inositol hexakisphosphate 3-kinase activity
molecular_function	GO:0000832	inositol hexakisphosphate 5-kinase activity