CSPI07G11280.1 (mRNA) Cucumber (PI 183967) v1

Overview


Name	CSPI07G11280.1
Type	mRNA
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	adenylate isopentenyltransferase 5, chloroplastic-like
Location	Chr7: 9486571 .. 9487590 (-)
Sequence length	1020
RNA-Seq Expression	CSPI07G11280.1
Synteny	CSPI07G11280.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGGACAACGGTGTTGAGTATCTTAGCAACAAGAAGCACAAAATCGTGTTCGTTATGGGCTCTACAGCTACCGGAAAATCAAAACTCTCCGTCGACTTAGCCACTTTCTTTCCATCCGAGATCATCAATTCCGACAAGATTCAGTTCTATAAAGGCCTCGACATAATCACCAACAAGATCAACCAATCCGATCGTCGCGGCGTTCCTCACCATCTCCTCGGCGTAATCCACGATCCAGACGCTGATTTAACTGCCGGAGAGTTCTGCAGTCTCGTCGAAGACGCAATCGCCGACGTTATCAGTCGAGGATGTCTTCCAATCGTCGTCGGAGGATCAAACAACTACATCGAGGCACTAGTTGAGAATCCGATTACTGATTTTCGGTCGCGGTTCGATTGTTGTTTCCTGTGGACCGATGTTGCTCTGCCTGTTCTGTACAAGTACATCGCGAAACGAGTGGATCAAATGGTGGAGTTAGGGTTAGTGGAAGAAGTTCGTGAGATGTTCGTTCCTGGTGCTGACTACAGCCGCGGAATCCGCCGCGCAATCGGCGCTCCAGAGCTAGATAGTTACTTCAAAGCAGAGAAGAACAACGAAGAAGAAACTTACAAGAATGATCTACTGAAATCGGGGATTCATGAAATTAAGGAAAACACCTGTAGACTGGCGCTCCGTCAATTCGGAAAGATCCACCGGCTGAGAGATGAGATCGGTTGGGGACTCCACAGGATCGACGCAACGGCGGTGTTTGAAAAGAGCGGAGAAGAGGCGGCGGATGAGTGGATGAATGGGGTTCTGAAACCGAGTTTGGGGATCGTTGGAGAATTCTTGAACGAAAAACAGAAAATAAATCAGATGGAAACGATGACGAAGAACCGATCAATGGGGGTTATGGATGAGTATGAAGAATTGAAAGCGAAATGGAATTTTGGATCAAATAACGCGATTGGATTTGGTTTAGTGATTGTATTTACGTCTTTAGGTTTGATGACGAATTGGATTTGGAGTTGGAAATGA

mRNA sequence

ATGAAGGACAACGGTGTTGAGTATCTTAGCAACAAGAAGCACAAAATCGTGTTCGTTATGGGCTCTACAGCTACCGGAAAATCAAAACTCTCCGTCGACTTAGCCACTTTCTTTCCATCCGAGATCATCAATTCCGACAAGATTCAGTTCTATAAAGGCCTCGACATAATCACCAACAAGATCAACCAATCCGATCGTCGCGGCGTTCCTCACCATCTCCTCGGCGTAATCCACGATCCAGACGCTGATTTAACTGCCGGAGAGTTCTGCAGTCTCGTCGAAGACGCAATCGCCGACGTTATCAGTCGAGGATGTCTTCCAATCGTCGTCGGAGGATCAAACAACTACATCGAGGCACTAGTTGAGAATCCGATTACTGATTTTCGGTCGCGGTTCGATTGTTGTTTCCTGTGGACCGATGTTGCTCTGCCTGTTCTGTACAAGTACATCGCGAAACGAGTGGATCAAATGGTGGAGTTAGGGTTAGTGGAAGAAGTTCGTGAGATGTTCGTTCCTGGTGCTGACTACAGCCGCGGAATCCGCCGCGCAATCGGCGCTCCAGAGCTAGATAGTTACTTCAAAGCAGAGAAGAACAACGAAGAAGAAACTTACAAGAATGATCTACTGAAATCGGGGATTCATGAAATTAAGGAAAACACCTGTAGACTGGCGCTCCGTCAATTCGGAAAGATCCACCGGCTGAGAGATGAGATCGGTTGGGGACTCCACAGGATCGACGCAACGGCGGTGTTTGAAAAGAGCGGAGAAGAGGCGGCGGATGAGTGGATGAATGGGGTTCTGAAACCGAGTTTGGGGATCGTTGGAGAATTCTTGAACGAAAAACAGAAAATAAATCAGATGGAAACGATGACGAAGAACCGATCAATGGGGGTTATGGATGAGTATGAAGAATTGAAAGCGAAATGGAATTTTGGATCAAATAACGCGATTGGATTTGGTTTAGTGATTGTATTTACGTCTTTAGGTTTGATGACGAATTGGATTTGGAGTTGGAAATGA

Coding sequence (CDS)

ATGAAGGACAACGGTGTTGAGTATCTTAGCAACAAGAAGCACAAAATCGTGTTCGTTATGGGCTCTACAGCTACCGGAAAATCAAAACTCTCCGTCGACTTAGCCACTTTCTTTCCATCCGAGATCATCAATTCCGACAAGATTCAGTTCTATAAAGGCCTCGACATAATCACCAACAAGATCAACCAATCCGATCGTCGCGGCGTTCCTCACCATCTCCTCGGCGTAATCCACGATCCAGACGCTGATTTAACTGCCGGAGAGTTCTGCAGTCTCGTCGAAGACGCAATCGCCGACGTTATCAGTCGAGGATGTCTTCCAATCGTCGTCGGAGGATCAAACAACTACATCGAGGCACTAGTTGAGAATCCGATTACTGATTTTCGGTCGCGGTTCGATTGTTGTTTCCTGTGGACCGATGTTGCTCTGCCTGTTCTGTACAAGTACATCGCGAAACGAGTGGATCAAATGGTGGAGTTAGGGTTAGTGGAAGAAGTTCGTGAGATGTTCGTTCCTGGTGCTGACTACAGCCGCGGAATCCGCCGCGCAATCGGCGCTCCAGAGCTAGATAGTTACTTCAAAGCAGAGAAGAACAACGAAGAAGAAACTTACAAGAATGATCTACTGAAATCGGGGATTCATGAAATTAAGGAAAACACCTGTAGACTGGCGCTCCGTCAATTCGGAAAGATCCACCGGCTGAGAGATGAGATCGGTTGGGGACTCCACAGGATCGACGCAACGGCGGTGTTTGAAAAGAGCGGAGAAGAGGCGGCGGATGAGTGGATGAATGGGGTTCTGAAACCGAGTTTGGGGATCGTTGGAGAATTCTTGAACGAAAAACAGAAAATAAATCAGATGGAAACGATGACGAAGAACCGATCAATGGGGGTTATGGATGAGTATGAAGAATTGAAAGCGAAATGGAATTTTGGATCAAATAACGCGATTGGATTTGGTTTAGTGATTGTATTTACGTCTTTAGGTTTGATGACGAATTGGATTTGGAGTTGGAAATGA

Protein sequence

MKDNGVEYLSNKKHKIVFVMGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNKINQSDRRGVPHHLLGVIHDPDADLTAGEFCSLVEDAIADVISRGCLPIVVGGSNNYIEALVENPITDFRSRFDCCFLWTDVALPVLYKYIAKRVDQMVELGLVEEVREMFVPGADYSRGIRRAIGAPELDSYFKAEKNNEEETYKNDLLKSGIHEIKENTCRLALRQFGKIHRLRDEIGWGLHRIDATAVFEKSGEEAADEWMNGVLKPSLGIVGEFLNEKQKINQMETMTKNRSMGVMDEYEELKAKWNFGSNNAIGFGLVIVFTSLGLMTNWIWSWK*

Homology

[Close]

BLAST of CSPI07G11280.1 vs. ExPASy Swiss-Prot
Match: Q94ID2 (Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT5 PE=1 SV=2)

HSP 1 Score: 295.8 bits (756), Expect = 6.2e-79
Identity = 145/269 (53.90%), Postives = 191/269 (71.00%), Query Frame = 0


Query: 12  KKHKIVFVMGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNKINQSDRRGVPH 71
           +K K+VFVMG+T TGKS+L++DLAT FP+EI+NSDKIQ YKGLDI+TNK+   +  GVPH
Sbjct: 31  RKDKVVFVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVYKGLDIVTNKVTPEESLGVPH 90

Query: 72  HLLGVIHDPDADLTAGEFCSLVEDAIADVISRGCLPIVVGGSNNYIEALVENPITDFRSR 131
           HLLG +HD   D TA +F      A+  ++ R  +PI+ GGSN+YIEALV N   DFR R
Sbjct: 91  HLLGTVHDTYEDFTAEDFQREAIRAVESIVQRDRVPIIAGGSNSYIEALV-NDCVDFRLR 150

Query: 132 FDCCFLWTDVALPVLYKYIAKRVDQMVELGLVEEVREMFVP-GADYSRGIRRAIGAPELD 191
           ++CCFLW DV+ PVL+ ++++RVD+MV++GLV+EVR +F P  +DYS GIRRAIG PELD
Sbjct: 151 YNCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVRRIFDPSSSDYSAGIRRAIGVPELD 210

Query: 192 SYFKAEKNNEEETYKNDLLKSGIHEIKENTCRLALRQFGKIHRLRDEIGWGLHRIDATAV 251
            + ++E  N        LL++ I +IKENTC LA RQ  KI RL  +  W +HR+DAT V
Sbjct: 211 EFLRSEMRNYPAETTERLLETAIEKIKENTCLLACRQLQKIQRLYKQWKWNMHRVDATEV 270

Query: 252 FEKSGEEAADEWMNGVLKPSLGIVGEFLN 280
           F + GEEA + W N V  PS   V +FL+
Sbjct: 271 FLRRGEEADEAWDNSVAHPSALAVEKFLS 298

[Close]

BLAST of CSPI07G11280.1 vs. ExPASy Swiss-Prot
Match: Q93WC9 (Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT3 PE=1 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 3.9e-73
Identity = 146/269 (54.28%), Postives = 188/269 (69.89%), Query Frame = 0


Query: 13  KHKIVFVMGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNKINQSDRRGVPHH 72
           K K+V +MG+T TGKS+LSVD+AT F +EIINSDKIQ ++GLDI+TNKI   +  GVPHH
Sbjct: 40  KDKVVVIMGATGTGKSRLSVDIATRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPHH 99

Query: 73  LLGVIHDPDADLTAGEFCSLVEDAIADVISRGCLPIVVGGSNNYIEALVENPITDFRSRF 132
           LLGV+  P+ADLTA  +C +   +I  V++RG LPI+VGGSN+Y+EALV++    FRSR+
Sbjct: 100 LLGVL-PPEADLTAANYCHMANLSIESVLNRGKLPIIVGGSNSYVEALVDDKENKFRSRY 159

Query: 133 DCCFLWTDVALPVLYKYIAKRVDQMVELGLVEEVREMF-VPGADYSRGIRRAIGAPELDS 192
           DCCFLW DVALPVL+ ++++RVD+MVE G+VEEVRE F    +DYSRGI++AIG PE D 
Sbjct: 160 DCCFLWVDVALPVLHGFVSERVDKMVESGMVEEVREFFDFSNSDYSRGIKKAIGFPEFDR 219

Query: 193 YFKAEKNNEEETYKNDLLKSGIHEIKENTCRLALRQFGKIHRLRDEIGWGLHRIDATAVF 252
           +F+ E+    E  + +LL   + EIK NT  LA RQ  KI RLR    W + R+DAT VF
Sbjct: 220 FFRNEQFLNVED-REELLSKVLEEIKRNTFELACRQREKIERLRKVKKWSIQRVDATPVF 279

Query: 253 EK--SGEEAADEWMNGVLKPSLGIVGEFL 279
            K  S  +A   W   V  PS   V  FL
Sbjct: 280 TKRRSKMDANVAWERLVAGPSTDTVSRFL 306

[Close]

BLAST of CSPI07G11280.1 vs. ExPASy Swiss-Prot
Match: Q94ID1 (Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=IPT7 PE=2 SV=2)

HSP 1 Score: 268.1 bits (684), Expect = 1.4e-70
Identity = 139/273 (50.92%), Postives = 183/273 (67.03%), Query Frame = 0


Query: 13  KHKIVFVMGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNKINQSDRRGVPHH 72
           K K++FVMG+T +GKS+L++DLAT F  EIINSDKIQ YKGLD++TNK+   + RGVPHH
Sbjct: 33  KEKVIFVMGATGSGKSRLAIDLATRFQGEIINSDKIQLYKGLDVLTNKVTPKECRGVPHH 92

Query: 73  LLGVIHDPDADLTAGEFCSLVEDAIADVISRGCLPIVVGGSNNYIEALVENPITDFRSRF 132
           LLGV      +LTA ++  L   AI+ + +   LPIV GGSN+YIEALV +      + +
Sbjct: 93  LLGVFDSEAGNLTATQYSRLASQAISKLSANNKLPIVAGGSNSYIEALVNHSSGFLLNNY 152

Query: 133 DCCFLWTDVALPVLYKYIAKRVDQMVELGLVEEVREMFVPGADYSRGIRRAIGAPELDSY 192
           DCCF+W DV+LPVL  +++KRVD+M+E GL+EEVRE+F P A+YS GIRRAIG PEL  Y
Sbjct: 153 DCCFIWVDVSLPVLNSFVSKRVDRMMEAGLLEEVREVFNPKANYSVGIRRAIGVPELHEY 212

Query: 193 FKAEKNNEEETYKNDLLKSGIHEIKENTCRLALRQFGKIHRLRDEIGWGLHRIDATAVFE 252
            + E   +  T K+ +L   +  IK+NT  LA RQ  KI RL  +    +HR+DAT VF 
Sbjct: 213 LRNESLVDRAT-KSKMLDVAVKNIKKNTEILACRQLKKIQRLHKKWKMSMHRVDATEVFL 272

Query: 253 KSGEEAADE-WMNGVLKPSLGIVGEFLNEKQKI 285
           K   E  DE W N V +PS  IV +F N   ++
Sbjct: 273 KRNVEEQDEAWENLVARPSERIVDKFYNNNNQL 304

[Close]

BLAST of CSPI07G11280.1 vs. ExPASy Swiss-Prot
Match: Q5GHF7 (Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1)

HSP 1 Score: 241.9 bits (616), Expect = 1.1e-62
Identity = 132/304 (43.42%), Postives = 183/304 (60.20%), Query Frame = 0


Query: 11  NKKHKIVFVMGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNKINQSDRRGVP 70
           ++K K++ +MG+T TGKS+LS+DLA  FP E+INSDK+Q YKGLDI TNKI+  DR GVP
Sbjct: 27  HRKEKLLVLMGATGTGKSRLSIDLAAHFPLEVINSDKMQVYKGLDITTNKISVPDRGGVP 86

Query: 71  HHLLGVIHDPDADLTAGEFCSLVEDAIADVISRGCLPIVVGGSNNYIEALV--------- 130
           HHLLG +     +LT  +F SL   A++++  R  LP++VGGSN++I AL+         
Sbjct: 87  HHLLGEVDPARGELTPADFRSLAGKAVSEITGRRKLPVLVGGSNSFIHALLVDRFDSSGP 146

Query: 131 ------ENPITDFRSRFDCCFLWTDVALPVLYKYIAKRVDQMVELGLVEEVREMFVP-GA 190
                  + +     R+DCCFLW DV++ VL  Y+AKRVD M+ELG+ +E+ E + P   
Sbjct: 147 GVFEEGSHSVVSSELRYDCCFLWVDVSVKVLTDYLAKRVDDMLELGMFDELAEFYSPEDE 206

Query: 191 DYSR------GIRRAIGAPELDSYFKAEKNNEEE-------TYKNDLLKSGIHEIKENTC 250
           D+        G+R+AIG PE D YF+  +  + E         +    +  +  IKENTC
Sbjct: 207 DHDEDSATRTGLRKAIGVPEFDRYFEKFRPGDVEGEDPGRDRVRRGAFEEAVRAIKENTC 266

Query: 251 RLALRQFGKIHRLRDEIGWGLHRIDAT-----AVFEKSGEEAADEWMNGVLKPSLGIVGE 281
            LA RQ GKI RL+   GW L R+DAT     A+   SGE+  + W   VL+PS+ IV  
Sbjct: 267 HLAKRQIGKILRLKG-AGWDLRRLDATESFRAAMTSDSGEKCTEIWEKQVLEPSVKIVSR 326

[Close]

BLAST of CSPI07G11280.1 vs. ExPASy Swiss-Prot
Match: Q94ID3 (Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT1 PE=1 SV=2)

HSP 1 Score: 237.3 bits (604), Expect = 2.6e-61
Identity = 135/295 (45.76%), Postives = 184/295 (62.37%), Query Frame = 0


Query: 11  NKKHKIVFVMGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNKINQSDRRGVP 70
           N+K K+V ++G+T  GKS+LSVDLAT FPSEIINSDKIQ Y+GL+I TN+I   DRRGVP
Sbjct: 62  NRKDKVVVILGATGAGKSRLSVDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVP 121

Query: 71  HHLLGVIHDPDADLTAGEFCSLVEDAIADVISRGCLPIVVGGSNNYIEALV----ENPIT 130
           HHLLGVI+    +LTAGEF S   + + ++ SR  +PI+ GGSN+++ AL+    +    
Sbjct: 122 HHLLGVINPEHGELTAGEFRSAASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFD 181

Query: 131 DFRS---------RFDCCFLWTDVALPVLYKYIAKRVDQMVELGLVEEVREMFVP---GA 190
            F S         R++CCF+W DV+  VLY+Y+ +RVD+M++ G+ EE+   + P   G 
Sbjct: 182 PFSSGSCLISSDLRYECCFIWVDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGL 241

Query: 191 DYSRGIRRAIGAPELDSYFKAEKNNEEETYKNDLLK-----SGIHEIKENTCRLALRQFG 250
           +   GIR+AIG PE D YFK E   E++  K D L+       + +IK NT  LA RQ  
Sbjct: 242 ETRFGIRKAIGVPEFDGYFK-EYPPEKKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQVK 301

Query: 251 KIHRLRDEIGWGLHRIDATAVFE----KSGEEA--ADEWMNGVLKPSLGIVGEFL 279
           KI  L+D  GW + R+DATA F+    KS  E    + W   VL+PS+ IV   L
Sbjct: 302 KIEMLKD-AGWEIERVDATASFKAVMMKSSSEKKWRENWEEQVLEPSVKIVKRHL 354

[Close]

BLAST of CSPI07G11280.1 vs. ExPASy TrEMBL
Match: A0A0A0K7L1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G253720 PE=4 SV=1)

HSP 1 Score: 692.2 bits (1785), Expect = 1.1e-195
Identity = 339/339 (100.00%), Postives = 339/339 (100.00%), Query Frame = 0


Query: 1   MKDNGVEYLSNKKHKIVFVMGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK 60
           MKDNGVEYLSNKKHKIVFVMGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK
Sbjct: 1   MKDNGVEYLSNKKHKIVFVMGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK 60

Query: 61  INQSDRRGVPHHLLGVIHDPDADLTAGEFCSLVEDAIADVISRGCLPIVVGGSNNYIEAL 120
           INQSDRRGVPHHLLGVIHDPDADLTAGEFCSLVEDAIADVISRGCLPIVVGGSNNYIEAL
Sbjct: 61  INQSDRRGVPHHLLGVIHDPDADLTAGEFCSLVEDAIADVISRGCLPIVVGGSNNYIEAL 120

Query: 121 VENPITDFRSRFDCCFLWTDVALPVLYKYIAKRVDQMVELGLVEEVREMFVPGADYSRGI 180
           VENPITDFRSRFDCCFLWTDVALPVLYKYIAKRVDQMVELGLVEEVREMFVPGADYSRGI
Sbjct: 121 VENPITDFRSRFDCCFLWTDVALPVLYKYIAKRVDQMVELGLVEEVREMFVPGADYSRGI 180

Query: 181 RRAIGAPELDSYFKAEKNNEEETYKNDLLKSGIHEIKENTCRLALRQFGKIHRLRDEIGW 240
           RRAIGAPELDSYFKAEKNNEEETYKNDLLKSGIHEIKENTCRLALRQFGKIHRLRDEIGW
Sbjct: 181 RRAIGAPELDSYFKAEKNNEEETYKNDLLKSGIHEIKENTCRLALRQFGKIHRLRDEIGW 240

Query: 241 GLHRIDATAVFEKSGEEAADEWMNGVLKPSLGIVGEFLNEKQKINQMETMTKNRSMGVMD 300
           GLHRIDATAVFEKSGEEAADEWMNGVLKPSLGIVGEFLNEKQKINQMETMTKNRSMGVMD
Sbjct: 241 GLHRIDATAVFEKSGEEAADEWMNGVLKPSLGIVGEFLNEKQKINQMETMTKNRSMGVMD 300

Query: 301 EYEELKAKWNFGSNNAIGFGLVIVFTSLGLMTNWIWSWK 340
           EYEELKAKWNFGSNNAIGFGLVIVFTSLGLMTNWIWSWK
Sbjct: 301 EYEELKAKWNFGSNNAIGFGLVIVFTSLGLMTNWIWSWK 339

[Close]

BLAST of CSPI07G11280.1 vs. ExPASy TrEMBL
Match: A0A5D3CPK4 (Adenylate isopentenyltransferase 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold179G00270 PE=4 SV=1)

HSP 1 Score: 631.3 bits (1627), Expect = 2.3e-177
Identity = 312/340 (91.76%), Postives = 323/340 (95.00%), Query Frame = 0


Query: 1   MKDNGVEYLSNKKHKIVFVMGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK 60
           MKD+GVEY SNKKHKIVFV+GSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK
Sbjct: 1   MKDSGVEYSSNKKHKIVFVLGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK 60

Query: 61  INQSDRRGVPHHLLGVIHDPDADLTAGEFCSLVEDAIADVISRGCLPIVVGGSNNYIEAL 120
           INQSDRRGVPHHLLGVIH+PDADLTAGEFC LVED+IADVISRGCLPIVVGGSNNYIEAL
Sbjct: 61  INQSDRRGVPHHLLGVIHEPDADLTAGEFCRLVEDSIADVISRGCLPIVVGGSNNYIEAL 120

Query: 121 VENPITDFRSRFDCCFLWTDVALPVLYKYIAKRVDQMVELGLVEEVREMFVPGADYSRGI 180
           VENPITDFRSRFDCCFLWTDVALPVLYKYI KRVDQMVELGLVEEVREMFVPGADYSRGI
Sbjct: 121 VENPITDFRSRFDCCFLWTDVALPVLYKYIGKRVDQMVELGLVEEVREMFVPGADYSRGI 180

Query: 181 RRAIGAPELDSYFKAEKNNEEETYKNDLLKSGIHEIKENTCRLALRQFGKIHRLRDEIGW 240
           RRAIGAPEL+SYFKAEKNNEEETYK +LLKS IHEIKENTC+LALRQFGKIHRLRDE+GW
Sbjct: 181 RRAIGAPELESYFKAEKNNEEETYKKELLKSAIHEIKENTCKLALRQFGKIHRLRDELGW 240

Query: 241 GLHRIDATAVFEKSGEEAADEWMNGVLKPSLGIVGEFLNEKQKINQMETMTKNRSMGVMD 300
           GLHRID TAVFEKSGEEAADEWMN VLKPSLGIVG+FLNEKQ+  QMETMTKNR MGVM+
Sbjct: 241 GLHRIDVTAVFEKSGEEAADEWMNAVLKPSLGIVGDFLNEKQRSYQMETMTKNR-MGVMN 300

Query: 301 E-YEELKAKWNFGSNNAIGFGLVIVFTSLGLMTNWIWSWK 340
             YEELK KWNFGSNNAIGFGLVI FTSL LM NWI SWK
Sbjct: 301 NGYEELKGKWNFGSNNAIGFGLVIGFTSLSLMANWILSWK 339

[Close]

BLAST of CSPI07G11280.1 vs. ExPASy TrEMBL
Match: A0A1S3BH37 (adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103489774 PE=4 SV=1)

HSP 1 Score: 630.2 bits (1624), Expect = 5.1e-177
Identity = 312/340 (91.76%), Postives = 323/340 (95.00%), Query Frame = 0


Query: 1   MKDNGVEYLSNKKHKIVFVMGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK 60
           MKD+GVEY SNKKHKIVFV+GSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK
Sbjct: 1   MKDSGVEYSSNKKHKIVFVLGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK 60

Query: 61  INQSDRRGVPHHLLGVIHDPDADLTAGEFCSLVEDAIADVISRGCLPIVVGGSNNYIEAL 120
           INQSDRRGVPHHLLGVIH+PDADLTAGEFC LVED+IADVISRGCLPIVVGGSNNYIEAL
Sbjct: 61  INQSDRRGVPHHLLGVIHEPDADLTAGEFCRLVEDSIADVISRGCLPIVVGGSNNYIEAL 120

Query: 121 VENPITDFRSRFDCCFLWTDVALPVLYKYIAKRVDQMVELGLVEEVREMFVPGADYSRGI 180
           VENPITDFRSRFDCCFLWTDVALPVLYKYI KRVDQMVELGLVEEV EMFVPGADYSRGI
Sbjct: 121 VENPITDFRSRFDCCFLWTDVALPVLYKYIGKRVDQMVELGLVEEVGEMFVPGADYSRGI 180

Query: 181 RRAIGAPELDSYFKAEKNNEEETYKNDLLKSGIHEIKENTCRLALRQFGKIHRLRDEIGW 240
           RRAIGAPEL+SYFKAEKNNEEETYK +LLKS IHEIKENTC+LALRQFGKIHRLRDE+GW
Sbjct: 181 RRAIGAPELESYFKAEKNNEEETYKKELLKSAIHEIKENTCKLALRQFGKIHRLRDELGW 240

Query: 241 GLHRIDATAVFEKSGEEAADEWMNGVLKPSLGIVGEFLNEKQKINQMETMTKNRSMGVMD 300
           GLHRID TAVFEKSGEEAADEWMN VLKPSLGIVG+FLNEKQ+  QMETMTKNR MGVM+
Sbjct: 241 GLHRIDVTAVFEKSGEEAADEWMNAVLKPSLGIVGDFLNEKQRSYQMETMTKNR-MGVMN 300

Query: 301 E-YEELKAKWNFGSNNAIGFGLVIVFTSLGLMTNWIWSWK 340
             YEELKAKWNFGSNNAIGFGLVI FTSL LM NWI SWK
Sbjct: 301 NGYEELKAKWNFGSNNAIGFGLVIGFTSLSLMANWILSWK 339

[Close]

BLAST of CSPI07G11280.1 vs. ExPASy TrEMBL
Match: A0A6J1I4L3 (adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111470933 PE=4 SV=1)

HSP 1 Score: 479.6 bits (1233), Expect = 1.1e-131
Identity = 238/306 (77.78%), Postives = 265/306 (86.60%), Query Frame = 0


Query: 3   DNGVEYLSNKKHKIVFVMGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNKIN 62
           +N VEYLSNKKHK++FVMG TATGKSKLSVDLATFFPSEIINSDKIQFY+GLDIITNKI 
Sbjct: 2   NNSVEYLSNKKHKVIFVMGCTATGKSKLSVDLATFFPSEIINSDKIQFYEGLDIITNKIA 61

Query: 63  QSDRRGVPHHLLGVIHDPDADLTAGEFCSLVEDAIADVISRGCLPIVVGGSNNYIEALVE 122
           +SDRRGVPHHLLGVI DPDAD TA EFC LVED+IA++I RG +PI+VGGSNNYIEALVE
Sbjct: 62  ESDRRGVPHHLLGVIQDPDADFTAAEFCRLVEDSIAEIIGRGRIPIIVGGSNNYIEALVE 121

Query: 123 NPITDFRSRFDCCFLWTDVALPVLYKYIAKRVDQMVELGLVEEVREMFVPGADYSRGIRR 182
           NPI DFRSRFDCCF+WTDVALPVL +YI+KRVDQMVE GLVEEVREM+VP ADYSRGIRR
Sbjct: 122 NPIADFRSRFDCCFVWTDVALPVLNRYISKRVDQMVEAGLVEEVREMYVPNADYSRGIRR 181

Query: 183 AIGAPELDSYFKAEKNNEEETYKNDLLKSGIHEIKENTCRLALRQFGKIHRLRDEIGWGL 242
           AIGAPE+DSYFK+EKN++       LLKS I EIK+NTC LALRQ GKIHRLRDE+GW +
Sbjct: 182 AIGAPEMDSYFKSEKNDD------SLLKSAIQEIKDNTCTLALRQRGKIHRLRDELGWRI 241

Query: 243 HRIDATAVFEKSGEEAADEWMNGVLKPSLGIVGEFLNEKQKINQMETMTKNRSMGVMDEY 302
           HRIDATAVFEKSGE+ AD WMN VLKPSL IVGEFLN++ K  ++ETM+KN        Y
Sbjct: 242 HRIDATAVFEKSGEDTADVWMNTVLKPSLDIVGEFLNKEHKDYRIETMSKN-GFTTNSGY 300

Query: 303 EELKAK 309
            ELKAK
Sbjct: 302 GELKAK 300

[Close]

BLAST of CSPI07G11280.1 vs. ExPASy TrEMBL
Match: A0A6J1GLR3 (adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111455496 PE=4 SV=1)

HSP 1 Score: 478.4 bits (1230), Expect = 2.5e-131
Identity = 237/306 (77.45%), Postives = 265/306 (86.60%), Query Frame = 0


Query: 3   DNGVEYLSNKKHKIVFVMGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNKIN 62
           ++ VEYLSNKKHK++FVMG TATGKSKLSVDLA FFPSEIINSDKIQFY+GLDIITNKI 
Sbjct: 5   NHSVEYLSNKKHKVIFVMGCTATGKSKLSVDLANFFPSEIINSDKIQFYEGLDIITNKIV 64

Query: 63  QSDRRGVPHHLLGVIHDPDADLTAGEFCSLVEDAIADVISRGCLPIVVGGSNNYIEALVE 122
           +SDRRGVPHHLLGVI DPDADLTA EFC LVED+IA++I RG +PI+VGGSNNYIEALVE
Sbjct: 65  ESDRRGVPHHLLGVIQDPDADLTAAEFCRLVEDSIAEIIGRGRIPIIVGGSNNYIEALVE 124

Query: 123 NPITDFRSRFDCCFLWTDVALPVLYKYIAKRVDQMVELGLVEEVREMFVPGADYSRGIRR 182
           NPI DFRSRFDCCF+WTDVALPVL +YI+KRVDQMVE GLVEEVREM+VP ADYSRGIRR
Sbjct: 125 NPIADFRSRFDCCFVWTDVALPVLNRYISKRVDQMVEAGLVEEVREMYVPNADYSRGIRR 184

Query: 183 AIGAPELDSYFKAEKNNEEETYKNDLLKSGIHEIKENTCRLALRQFGKIHRLRDEIGWGL 242
           AIGAPE+DSYFK+EKN++       LLKS I EIK+NTC LALRQ GKIHRLRDE+GWG+
Sbjct: 185 AIGAPEMDSYFKSEKNDD------SLLKSAIQEIKDNTCTLALRQRGKIHRLRDELGWGI 244

Query: 243 HRIDATAVFEKSGEEAADEWMNGVLKPSLGIVGEFLNEKQKINQMETMTKNRSMGVMDEY 302
           HRIDATAVFE SGE+ AD WMN VLKPSL IVG+FLN++ K  ++ETMTKN        Y
Sbjct: 245 HRIDATAVFENSGEDTADVWMNTVLKPSLEIVGKFLNKEHKDYRIETMTKN-GFTTNSSY 303

Query: 303 EELKAK 309
            ELKAK
Sbjct: 305 GELKAK 303

[Close]

BLAST of CSPI07G11280.1 vs. NCBI nr
Match: XP_004148309.1 (adenylate isopentenyltransferase 5, chloroplastic [Cucumis sativus])

HSP 1 Score: 692.2 bits (1785), Expect = 2.3e-195
Identity = 339/339 (100.00%), Postives = 339/339 (100.00%), Query Frame = 0


Query: 1   MKDNGVEYLSNKKHKIVFVMGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK 60
           MKDNGVEYLSNKKHKIVFVMGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK
Sbjct: 1   MKDNGVEYLSNKKHKIVFVMGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK 60

Query: 61  INQSDRRGVPHHLLGVIHDPDADLTAGEFCSLVEDAIADVISRGCLPIVVGGSNNYIEAL 120
           INQSDRRGVPHHLLGVIHDPDADLTAGEFCSLVEDAIADVISRGCLPIVVGGSNNYIEAL
Sbjct: 61  INQSDRRGVPHHLLGVIHDPDADLTAGEFCSLVEDAIADVISRGCLPIVVGGSNNYIEAL 120

Query: 121 VENPITDFRSRFDCCFLWTDVALPVLYKYIAKRVDQMVELGLVEEVREMFVPGADYSRGI 180
           VENPITDFRSRFDCCFLWTDVALPVLYKYIAKRVDQMVELGLVEEVREMFVPGADYSRGI
Sbjct: 121 VENPITDFRSRFDCCFLWTDVALPVLYKYIAKRVDQMVELGLVEEVREMFVPGADYSRGI 180

Query: 181 RRAIGAPELDSYFKAEKNNEEETYKNDLLKSGIHEIKENTCRLALRQFGKIHRLRDEIGW 240
           RRAIGAPELDSYFKAEKNNEEETYKNDLLKSGIHEIKENTCRLALRQFGKIHRLRDEIGW
Sbjct: 181 RRAIGAPELDSYFKAEKNNEEETYKNDLLKSGIHEIKENTCRLALRQFGKIHRLRDEIGW 240

Query: 241 GLHRIDATAVFEKSGEEAADEWMNGVLKPSLGIVGEFLNEKQKINQMETMTKNRSMGVMD 300
           GLHRIDATAVFEKSGEEAADEWMNGVLKPSLGIVGEFLNEKQKINQMETMTKNRSMGVMD
Sbjct: 241 GLHRIDATAVFEKSGEEAADEWMNGVLKPSLGIVGEFLNEKQKINQMETMTKNRSMGVMD 300

Query: 301 EYEELKAKWNFGSNNAIGFGLVIVFTSLGLMTNWIWSWK 340
           EYEELKAKWNFGSNNAIGFGLVIVFTSLGLMTNWIWSWK
Sbjct: 301 EYEELKAKWNFGSNNAIGFGLVIVFTSLGLMTNWIWSWK 339

[Close]

BLAST of CSPI07G11280.1 vs. NCBI nr
Match: KAA0036515.1 (adenylate isopentenyltransferase 5 [Cucumis melo var. makuwa] >TYK13465.1 adenylate isopentenyltransferase 5 [Cucumis melo var. makuwa])

HSP 1 Score: 631.3 bits (1627), Expect = 4.7e-177
Identity = 312/340 (91.76%), Postives = 323/340 (95.00%), Query Frame = 0


Query: 1   MKDNGVEYLSNKKHKIVFVMGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK 60
           MKD+GVEY SNKKHKIVFV+GSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK
Sbjct: 1   MKDSGVEYSSNKKHKIVFVLGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK 60

Query: 61  INQSDRRGVPHHLLGVIHDPDADLTAGEFCSLVEDAIADVISRGCLPIVVGGSNNYIEAL 120
           INQSDRRGVPHHLLGVIH+PDADLTAGEFC LVED+IADVISRGCLPIVVGGSNNYIEAL
Sbjct: 61  INQSDRRGVPHHLLGVIHEPDADLTAGEFCRLVEDSIADVISRGCLPIVVGGSNNYIEAL 120

Query: 121 VENPITDFRSRFDCCFLWTDVALPVLYKYIAKRVDQMVELGLVEEVREMFVPGADYSRGI 180
           VENPITDFRSRFDCCFLWTDVALPVLYKYI KRVDQMVELGLVEEVREMFVPGADYSRGI
Sbjct: 121 VENPITDFRSRFDCCFLWTDVALPVLYKYIGKRVDQMVELGLVEEVREMFVPGADYSRGI 180

Query: 181 RRAIGAPELDSYFKAEKNNEEETYKNDLLKSGIHEIKENTCRLALRQFGKIHRLRDEIGW 240
           RRAIGAPEL+SYFKAEKNNEEETYK +LLKS IHEIKENTC+LALRQFGKIHRLRDE+GW
Sbjct: 181 RRAIGAPELESYFKAEKNNEEETYKKELLKSAIHEIKENTCKLALRQFGKIHRLRDELGW 240

Query: 241 GLHRIDATAVFEKSGEEAADEWMNGVLKPSLGIVGEFLNEKQKINQMETMTKNRSMGVMD 300
           GLHRID TAVFEKSGEEAADEWMN VLKPSLGIVG+FLNEKQ+  QMETMTKNR MGVM+
Sbjct: 241 GLHRIDVTAVFEKSGEEAADEWMNAVLKPSLGIVGDFLNEKQRSYQMETMTKNR-MGVMN 300

Query: 301 E-YEELKAKWNFGSNNAIGFGLVIVFTSLGLMTNWIWSWK 340
             YEELK KWNFGSNNAIGFGLVI FTSL LM NWI SWK
Sbjct: 301 NGYEELKGKWNFGSNNAIGFGLVIGFTSLSLMANWILSWK 339

[Close]

BLAST of CSPI07G11280.1 vs. NCBI nr
Match: XP_008447303.1 (PREDICTED: adenylate isopentenyltransferase 5, chloroplastic-like [Cucumis melo])

HSP 1 Score: 630.2 bits (1624), Expect = 1.1e-176
Identity = 312/340 (91.76%), Postives = 323/340 (95.00%), Query Frame = 0


Query: 1   MKDNGVEYLSNKKHKIVFVMGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK 60
           MKD+GVEY SNKKHKIVFV+GSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK
Sbjct: 1   MKDSGVEYSSNKKHKIVFVLGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK 60

Query: 61  INQSDRRGVPHHLLGVIHDPDADLTAGEFCSLVEDAIADVISRGCLPIVVGGSNNYIEAL 120
           INQSDRRGVPHHLLGVIH+PDADLTAGEFC LVED+IADVISRGCLPIVVGGSNNYIEAL
Sbjct: 61  INQSDRRGVPHHLLGVIHEPDADLTAGEFCRLVEDSIADVISRGCLPIVVGGSNNYIEAL 120

Query: 121 VENPITDFRSRFDCCFLWTDVALPVLYKYIAKRVDQMVELGLVEEVREMFVPGADYSRGI 180
           VENPITDFRSRFDCCFLWTDVALPVLYKYI KRVDQMVELGLVEEV EMFVPGADYSRGI
Sbjct: 121 VENPITDFRSRFDCCFLWTDVALPVLYKYIGKRVDQMVELGLVEEVGEMFVPGADYSRGI 180

Query: 181 RRAIGAPELDSYFKAEKNNEEETYKNDLLKSGIHEIKENTCRLALRQFGKIHRLRDEIGW 240
           RRAIGAPEL+SYFKAEKNNEEETYK +LLKS IHEIKENTC+LALRQFGKIHRLRDE+GW
Sbjct: 181 RRAIGAPELESYFKAEKNNEEETYKKELLKSAIHEIKENTCKLALRQFGKIHRLRDELGW 240

Query: 241 GLHRIDATAVFEKSGEEAADEWMNGVLKPSLGIVGEFLNEKQKINQMETMTKNRSMGVMD 300
           GLHRID TAVFEKSGEEAADEWMN VLKPSLGIVG+FLNEKQ+  QMETMTKNR MGVM+
Sbjct: 241 GLHRIDVTAVFEKSGEEAADEWMNAVLKPSLGIVGDFLNEKQRSYQMETMTKNR-MGVMN 300

Query: 301 E-YEELKAKWNFGSNNAIGFGLVIVFTSLGLMTNWIWSWK 340
             YEELKAKWNFGSNNAIGFGLVI FTSL LM NWI SWK
Sbjct: 301 NGYEELKAKWNFGSNNAIGFGLVIGFTSLSLMANWILSWK 339

[Close]

BLAST of CSPI07G11280.1 vs. NCBI nr
Match: KAE8646129.1 (hypothetical protein Csa_016307 [Cucumis sativus])

HSP 1 Score: 604.0 bits (1556), Expect = 8.1e-169
Identity = 299/310 (96.45%), Postives = 302/310 (97.42%), Query Frame = 0


Query: 1   MKDNGVEYLSNKKHKIVFVMGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK 60
           MKDNGVEYLSNKKHKIVFVMGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK
Sbjct: 7   MKDNGVEYLSNKKHKIVFVMGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK 66

Query: 61  INQSDRRGVPHHLLGVIHDPDADLTAGEFCSLVEDAIADVISRGCLPIVVGGSNNYIEAL 120
           INQSDRRGVPHHLLGVIHDPDADLTAGEFCSLVEDAIADVISRGCLPIVVGGSNNYIEAL
Sbjct: 67  INQSDRRGVPHHLLGVIHDPDADLTAGEFCSLVEDAIADVISRGCLPIVVGGSNNYIEAL 126

Query: 121 VENPITDFRSRFDCCFLWTDVALPVLYKYIAKRVDQMVELGLVEEVREMFVPGADYSRGI 180
           VENPITDFRSRFDCCFLWTDVALPVLYKYIAKRVDQMVELGLVEEVREMFVPGADYSRGI
Sbjct: 127 VENPITDFRSRFDCCFLWTDVALPVLYKYIAKRVDQMVELGLVEEVREMFVPGADYSRGI 186

Query: 181 RRAIGAPELDSYFKAEKNNEEETYKNDLLKSGIHEIKENTCRLALRQFGKIHRLRDEIGW 240
           RRAIGAPELDSYFKAEKNNEEETYKNDLLKSGIHEIKENTCRLALRQFGKIHRLRDEIGW
Sbjct: 187 RRAIGAPELDSYFKAEKNNEEETYKNDLLKSGIHEIKENTCRLALRQFGKIHRLRDEIGW 246

Query: 241 GLHRIDATAVFEKSGEEAADEWMNGVLKPSLGIVGEFLNEKQKINQMETMTKNRSMGVMD 300
           GLHRIDATAVFEKSGEEAADEWMNGVLKPSLGIVGEFLNEKQKINQMETMTKNRSMG   
Sbjct: 247 GLHRIDATAVFEKSGEEAADEWMNGVLKPSLGIVGEFLNEKQKINQMETMTKNRSMGSQK 306

Query: 301 EYEELKAKWN 311
            ++ L +  N
Sbjct: 307 PHQALLSLLN 316

[Close]

BLAST of CSPI07G11280.1 vs. NCBI nr
Match: XP_038888774.1 (adenylate isopentenyltransferase 5, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 599.7 bits (1545), Expect = 1.5e-167
Identity = 296/339 (87.32%), Postives = 317/339 (93.51%), Query Frame = 0


Query: 1   MKDNGVEYLSNKKHKIVFVMGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK 60
           MKD+ VEYLSNKKHKIVFVMGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK
Sbjct: 1   MKDS-VEYLSNKKHKIVFVMGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNK 60

Query: 61  INQSDRRGVPHHLLGVIHDPDADLTAGEFCSLVEDAIADVISRGCLPIVVGGSNNYIEAL 120
           I +SDRRGVPHHLLGVI DPDADLTAGEFC LVED+IAD+IS G LPI+VGGSNNYIEAL
Sbjct: 61  ITESDRRGVPHHLLGVIDDPDADLTAGEFCRLVEDSIADIISHGRLPIIVGGSNNYIEAL 120

Query: 121 VENPITDFRSRFDCCFLWTDVALPVLYKYIAKRVDQMVELGLVEEVREMFVPGADYSRGI 180
           VENPITDFRSRFDCCFLW DVALPVLYKYI KRVDQMVELGLVEEVR+MFVPGADYSRGI
Sbjct: 121 VENPITDFRSRFDCCFLWIDVALPVLYKYIGKRVDQMVELGLVEEVRDMFVPGADYSRGI 180

Query: 181 RRAIGAPELDSYFKAEKNNEEETYKNDLLKSGIHEIKENTCRLALRQFGKIHRLRDEIGW 240
           RRAIGAPELDSYFKAEKNNEEE+YKNDLLKSGIHEIKENTC+LALRQFGKIHRLRDE+GW
Sbjct: 181 RRAIGAPELDSYFKAEKNNEEESYKNDLLKSGIHEIKENTCKLALRQFGKIHRLRDELGW 240

Query: 241 GLHRIDATAVFEKSGEEAADEWMNGVLKPSLGIVGEFLNEKQKINQMETMTKNRSMGVMD 300
           GLHR+DATAVFEKSGEEAADEWMN VLKPSLGIVG+FLNEKQK  Q+E + KNR + +M+
Sbjct: 241 GLHRVDATAVFEKSGEEAADEWMNAVLKPSLGIVGDFLNEKQKDYQIEAVIKNR-LAIMN 300

Query: 301 EYEELKAKWNFGSNNAIGFGLVIVFTSLGLMTNWIWSWK 340
            YEELKAK NFGSNNA+G+GLVI F + GL+TNW+ S K
Sbjct: 301 GYEELKAKMNFGSNNAVGYGLVIGFAAFGLITNWVCSSK 337

[Close]

BLAST of CSPI07G11280.1 vs. TAIR 10
Match: AT5G19040.1 (isopentenyltransferase 5 )

HSP 1 Score: 295.8 bits (756), Expect = 4.4e-80
Identity = 145/269 (53.90%), Postives = 191/269 (71.00%), Query Frame = 0


Query: 12  KKHKIVFVMGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNKINQSDRRGVPH 71
           +K K+VFVMG+T TGKS+L++DLAT FP+EI+NSDKIQ YKGLDI+TNK+   +  GVPH
Sbjct: 31  RKDKVVFVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVYKGLDIVTNKVTPEESLGVPH 90

Query: 72  HLLGVIHDPDADLTAGEFCSLVEDAIADVISRGCLPIVVGGSNNYIEALVENPITDFRSR 131
           HLLG +HD   D TA +F      A+  ++ R  +PI+ GGSN+YIEALV N   DFR R
Sbjct: 91  HLLGTVHDTYEDFTAEDFQREAIRAVESIVQRDRVPIIAGGSNSYIEALV-NDCVDFRLR 150

Query: 132 FDCCFLWTDVALPVLYKYIAKRVDQMVELGLVEEVREMFVP-GADYSRGIRRAIGAPELD 191
           ++CCFLW DV+ PVL+ ++++RVD+MV++GLV+EVR +F P  +DYS GIRRAIG PELD
Sbjct: 151 YNCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVRRIFDPSSSDYSAGIRRAIGVPELD 210

Query: 192 SYFKAEKNNEEETYKNDLLKSGIHEIKENTCRLALRQFGKIHRLRDEIGWGLHRIDATAV 251
            + ++E  N        LL++ I +IKENTC LA RQ  KI RL  +  W +HR+DAT V
Sbjct: 211 EFLRSEMRNYPAETTERLLETAIEKIKENTCLLACRQLQKIQRLYKQWKWNMHRVDATEV 270

Query: 252 FEKSGEEAADEWMNGVLKPSLGIVGEFLN 280
           F + GEEA + W N V  PS   V +FL+
Sbjct: 271 FLRRGEEADEAWDNSVAHPSALAVEKFLS 298

[Close]

BLAST of CSPI07G11280.1 vs. TAIR 10
Match: AT3G63110.1 (isopentenyltransferase 3 )

HSP 1 Score: 276.6 bits (706), Expect = 2.8e-74
Identity = 146/269 (54.28%), Postives = 188/269 (69.89%), Query Frame = 0


Query: 13  KHKIVFVMGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNKINQSDRRGVPHH 72
           K K+V +MG+T TGKS+LSVD+AT F +EIINSDKIQ ++GLDI+TNKI   +  GVPHH
Sbjct: 40  KDKVVVIMGATGTGKSRLSVDIATRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPHH 99

Query: 73  LLGVIHDPDADLTAGEFCSLVEDAIADVISRGCLPIVVGGSNNYIEALVENPITDFRSRF 132
           LLGV+  P+ADLTA  +C +   +I  V++RG LPI+VGGSN+Y+EALV++    FRSR+
Sbjct: 100 LLGVL-PPEADLTAANYCHMANLSIESVLNRGKLPIIVGGSNSYVEALVDDKENKFRSRY 159

Query: 133 DCCFLWTDVALPVLYKYIAKRVDQMVELGLVEEVREMF-VPGADYSRGIRRAIGAPELDS 192
           DCCFLW DVALPVL+ ++++RVD+MVE G+VEEVRE F    +DYSRGI++AIG PE D 
Sbjct: 160 DCCFLWVDVALPVLHGFVSERVDKMVESGMVEEVREFFDFSNSDYSRGIKKAIGFPEFDR 219

Query: 193 YFKAEKNNEEETYKNDLLKSGIHEIKENTCRLALRQFGKIHRLRDEIGWGLHRIDATAVF 252
           +F+ E+    E  + +LL   + EIK NT  LA RQ  KI RLR    W + R+DAT VF
Sbjct: 220 FFRNEQFLNVED-REELLSKVLEEIKRNTFELACRQREKIERLRKVKKWSIQRVDATPVF 279

Query: 253 EK--SGEEAADEWMNGVLKPSLGIVGEFL 279
            K  S  +A   W   V  PS   V  FL
Sbjct: 280 TKRRSKMDANVAWERLVAGPSTDTVSRFL 306

[Close]

BLAST of CSPI07G11280.1 vs. TAIR 10
Match: AT3G23630.1 (isopentenyltransferase 7 )

HSP 1 Score: 268.1 bits (684), Expect = 9.8e-72
Identity = 139/273 (50.92%), Postives = 183/273 (67.03%), Query Frame = 0


Query: 13  KHKIVFVMGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNKINQSDRRGVPHH 72
           K K++FVMG+T +GKS+L++DLAT F  EIINSDKIQ YKGLD++TNK+   + RGVPHH
Sbjct: 33  KEKVIFVMGATGSGKSRLAIDLATRFQGEIINSDKIQLYKGLDVLTNKVTPKECRGVPHH 92

Query: 73  LLGVIHDPDADLTAGEFCSLVEDAIADVISRGCLPIVVGGSNNYIEALVENPITDFRSRF 132
           LLGV      +LTA ++  L   AI+ + +   LPIV GGSN+YIEALV +      + +
Sbjct: 93  LLGVFDSEAGNLTATQYSRLASQAISKLSANNKLPIVAGGSNSYIEALVNHSSGFLLNNY 152

Query: 133 DCCFLWTDVALPVLYKYIAKRVDQMVELGLVEEVREMFVPGADYSRGIRRAIGAPELDSY 192
           DCCF+W DV+LPVL  +++KRVD+M+E GL+EEVRE+F P A+YS GIRRAIG PEL  Y
Sbjct: 153 DCCFIWVDVSLPVLNSFVSKRVDRMMEAGLLEEVREVFNPKANYSVGIRRAIGVPELHEY 212

Query: 193 FKAEKNNEEETYKNDLLKSGIHEIKENTCRLALRQFGKIHRLRDEIGWGLHRIDATAVFE 252
            + E   +  T K+ +L   +  IK+NT  LA RQ  KI RL  +    +HR+DAT VF 
Sbjct: 213 LRNESLVDRAT-KSKMLDVAVKNIKKNTEILACRQLKKIQRLHKKWKMSMHRVDATEVFL 272

Query: 253 KSGEEAADE-WMNGVLKPSLGIVGEFLNEKQKI 285
           K   E  DE W N V +PS  IV +F N   ++
Sbjct: 273 KRNVEEQDEAWENLVARPSERIVDKFYNNNNQL 304

[Close]

BLAST of CSPI07G11280.1 vs. TAIR 10
Match: AT1G68460.1 (isopentenyltransferase 1 )

HSP 1 Score: 237.3 bits (604), Expect = 1.9e-62
Identity = 135/295 (45.76%), Postives = 184/295 (62.37%), Query Frame = 0


Query: 11  NKKHKIVFVMGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNKINQSDRRGVP 70
           N+K K+V ++G+T  GKS+LSVDLAT FPSEIINSDKIQ Y+GL+I TN+I   DRRGVP
Sbjct: 62  NRKDKVVVILGATGAGKSRLSVDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVP 121

Query: 71  HHLLGVIHDPDADLTAGEFCSLVEDAIADVISRGCLPIVVGGSNNYIEALV----ENPIT 130
           HHLLGVI+    +LTAGEF S   + + ++ SR  +PI+ GGSN+++ AL+    +    
Sbjct: 122 HHLLGVINPEHGELTAGEFRSAASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFD 181

Query: 131 DFRS---------RFDCCFLWTDVALPVLYKYIAKRVDQMVELGLVEEVREMFVP---GA 190
            F S         R++CCF+W DV+  VLY+Y+ +RVD+M++ G+ EE+   + P   G 
Sbjct: 182 PFSSGSCLISSDLRYECCFIWVDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGL 241

Query: 191 DYSRGIRRAIGAPELDSYFKAEKNNEEETYKNDLLK-----SGIHEIKENTCRLALRQFG 250
           +   GIR+AIG PE D YFK E   E++  K D L+       + +IK NT  LA RQ  
Sbjct: 242 ETRFGIRKAIGVPEFDGYFK-EYPPEKKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQVK 301

Query: 251 KIHRLRDEIGWGLHRIDATAVFE----KSGEEA--ADEWMNGVLKPSLGIVGEFL 279
           KI  L+D  GW + R+DATA F+    KS  E    + W   VL+PS+ IV   L
Sbjct: 302 KIEMLKD-AGWEIERVDATASFKAVMMKSSSEKKWRENWEEQVLEPSVKIVKRHL 354

[Close]

BLAST of CSPI07G11280.1 vs. TAIR 10
Match: AT3G19160.1 (ATP/ADP isopentenyltransferases )

HSP 1 Score: 225.7 bits (574), Expect = 5.6e-59
Identity = 124/288 (43.06%), Postives = 178/288 (61.81%), Query Frame = 0


Query: 13  KHKIVFVMGSTATGKSKLSVDLATFFPSEIINSDKIQFYKGLDIITNKINQSDRRGVPHH 72
           K K+V +MG+T +GKS LS+DLAT F  EI+NSDKIQFY GL + TN+++  +R GVPHH
Sbjct: 42  KQKVVVIMGATGSGKSCLSIDLATRFSGEIVNSDKIQFYDGLKVTTNQMSILERCGVPHH 101

Query: 73  LLGVIHDPDADLTAGEFCSLVEDAIADVISRGCLPIVVGGSNNYIEALVEN-------PI 132
           LLG +   D++LT  EF SL   +I+++ +RG LPI+ GGSN++I AL+ +       P 
Sbjct: 102 LLGELPPDDSELTTSEFRSLASRSISEITARGNLPIIAGGSNSFIHALLVDRFDPKTYPF 161

Query: 133 TDFRS-----RFDCCFLWTDVALPVLYKYIAKRVDQMVELGLVEEVREMFVP----GADY 192
           +   S     R++CCFLW DV++ VL++Y++KRVDQM+E G+ EE+   + P     A  
Sbjct: 162 SSETSISSGLRYECCFLWVDVSVSVLFEYLSKRVDQMMESGMFEELAGFYDPRYSGSAIR 221

Query: 193 SRGIRRAIGAPELDSYF------KAEKNNEEETYKNDLLKSGIHEIKENTCRLALRQFGK 252
           + GI + IG PE D YF      + +K +E +  +       + EIKENT RLA +Q  +
Sbjct: 222 AHGIHKTIGIPEFDRYFSLYPPERKQKMSEWDQARKGAYDEAVQEIKENTWRLAKKQIER 281

Query: 253 IHRLRDEIGWGLHRIDATAVFEKSGEEAADEWMNGVLKPSLGIVGEFL 279
           I +L+   GW + R+DAT  F +S  E    W N VL  S+ +V  FL
Sbjct: 282 IMKLKSS-GWDIQRLDATPSFGRSSREI---WDNTVLDESIKVVKRFL 325

The following BLAST results are available for this feature:

BLAST of CSPI07G11280.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94ID2	6.2e-79	53.90	Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]
Q93WC9	3.9e-73	54.28	Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]
Q94ID1	1.4e-70	50.92	Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=370...	[more]
Q5GHF7	1.1e-62	43.42	Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1	[more]
Q94ID3	2.6e-61	45.76	Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]

BLAST of CSPI07G11280.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K7L1	1.1e-195	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G253720 PE=4 SV=1	[more]
A0A5D3CPK4	2.3e-177	91.76	Adenylate isopentenyltransferase 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3BH37	5.1e-177	91.76	adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucumis melo OX=3656 G...	[more]
A0A6J1I4L3	1.1e-131	77.78	adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita maxima OX=36...	[more]
A0A6J1GLR3	2.5e-131	77.45	adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita moschata OX=...	[more]

BLAST of CSPI07G11280.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004148309.1	2.3e-195	100.00	adenylate isopentenyltransferase 5, chloroplastic [Cucumis sativus]	[more]
KAA0036515.1	4.7e-177	91.76	adenylate isopentenyltransferase 5 [Cucumis melo var. makuwa] >TYK13465.1 adenyl...	[more]
XP_008447303.1	1.1e-176	91.76	PREDICTED: adenylate isopentenyltransferase 5, chloroplastic-like [Cucumis melo]	[more]
KAE8646129.1	8.1e-169	96.45	hypothetical protein Csa_016307 [Cucumis sativus]	[more]
XP_038888774.1	1.5e-167	87.32	adenylate isopentenyltransferase 5, chloroplastic-like [Benincasa hispida]	[more]

BLAST of CSPI07G11280.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G19040.1	4.4e-80	53.90	isopentenyltransferase 5	[more]
AT3G63110.1	2.8e-74	54.28	isopentenyltransferase 3	[more]
AT3G23630.1	9.8e-72	50.92	isopentenyltransferase 7	[more]
AT1G68460.1	1.9e-62	45.76	isopentenyltransferase 1	[more]
AT3G19160.1	5.6e-59	43.06	ATP/ADP isopentenyltransferases	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.10.287.890	Crystal structure of tRNA isopentenylpyrophosphate transferase (bh2366) domain	coord: 143..242 e-value: 2.8E-105 score: 353.3
None	No IPR available	PFAM	PF01715	IPPT	coord: 49..123 e-value: 4.9E-20 score: 72.3 coord: 131..228 e-value: 7.9E-12 score: 45.4
None	No IPR available	PANTHER	PTHR11088:SF67	BINDING PROTEIN, PUTATIVE-RELATED	coord: 8..282
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 13..278 e-value: 2.8E-105 score: 353.3
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 16..127
IPR039657	Dimethylallyltransferase	PANTHER	PTHR11088	TRNA DIMETHYLALLYLTRANSFERASE	coord: 8..282

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI07G11280	CSPI07G11280	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI07G11280.1.cds1	CSPI07G11280.1.cds1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI07G11280.1	CSPI07G11280.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009691	cytokinin biosynthetic process
biological_process	GO:0006400	tRNA modification
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0052381	tRNA dimethylallyltransferase activity