CSPI04G20320.3 (mRNA) Cucumber (PI 183967) v1

Overview


Name	CSPI04G20320.3
Type	mRNA
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	RanBP2-type domain-containing protein
Location	Chr4: 18431180 .. 18433528 (+)
Sequence length	1530
RNA-Seq Expression	CSPI04G20320.3
Synteny	CSPI04G20320.3

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AACAGTTTGGTACCCTAGTTATATCTATATATTTTTTTTGAAACAATATTTTCTTGAGTTGCAATGCATGAGAATGCTGCTCTTTCCTCTTTGTATTATTGTTCCTACTTTAACCAACTTTTTATTGGATACTATAGGGAATAAGCACAGGCAGGAATGTTACCACTGCTAGCTTACCAAGTAGTGAGCCAATACTACAGGCTCCAAGGGAACCCGATCACTCTGGGGCTCCTTGCCTCTAATACTCTCATTCATCTAAGGCCCAATTTTCTTCATCATATCATCCCTTCCATAGATGAGGTCTGGTTTAATGCACATCTCATCTTCAAGGTGACTATTCTGGCTAATAATCTAATTCTATTCTGCTGTTCTCTGTTTAAGACTTAACTGCACTCTCTATGTTAGAGTTAATTGGTGATGATTATAAAAATTTAATGAGAGATCTTTCTGCTGAGGACTTTCCTATTCATTAATACATCGGTAGGGAAGTCCTAAACAGAATGGCACGTTACTTTCTCTGGAATAAAGCATTGCTGTCTCATTGAGAAATTGGTATCATGTTTCTTTTTTGATCAGCTGTATAAATAATCTTGTAGTTTCCTTAGATACTGACTACCTGCTTTATAGCATCATTGAAGCGAATAGGTCTTTATTTATTTATTGAACTTTTTAGCTGTGAAGTCATTTTTCATTGAGAAAAATGGAAGAAATAAGTAGATACTAGTTGAATTTGTATGCTTTAACCATAAGAAATGGTTCTTGTATGATGTTGAACGTTACAAAACACATATAGATTGGGTAGCCCCCACATAACTGTGTAATTTGTGTCCTTACAAATCGTCCTCATAGTGAGGTTGTTGTATGATGGCCTCAAATTATAGTTAAACTTTTATTTTGAGAAAAATGAAAAGCTTTTAAGAGTTTGCTTTGATTTTTGTACAATATTCACAGTTTCTTTCTTTTCAGTATTGTACTTCTTTAATCCATCTTGATGAGTATATACCTAACAATTTTGAACTCCTGTAGGATTATTTTCTTTTTTGTTGTTTATTAACTTGTTTGCATGTCGTTTTTGAATGAGATAATGTGATATTGGCATTCCTGTTACTGTCATAATATTGGTATCATTTGCATTTCCCACTTTGCCAGCACAAGGACTTGAAGCGTTTTTTACTATCACCATTCTACCATGTGGGTGACCCTCACCTTGTCTACAACATGATATCTCTACTATGGAAAGGCACTCAGTTGGAAACATCAATGGGTAGCATCGAATTTGCTTCAATGGTTGGTGCATTACTCATAATGTCGCAGGGAATTACCTTGTTGCTCATCAAATCCTTACTGATATTCTTTAATTATGGGAGGCCTTACTACTATGAATATTCTGTTGGGTTTTCCGGTGTACTTTTTGCTATGAAAGTTGTTCTTGGTTCTGAAACTGACAGCTACACTTACGTTTATGGTCTAAGAGTACCATCAAGTCATGCTGCATGGTTGGAACTAATACTTATCCAAATGGTTTCACCTGGTGTCTCTTTCCTTGGTCATCTTGGTGGTATACTTGCTGGTCTTCTCTTTTTGCGTCTGAAGGGTACGAATTCAGGGTCGGATCCATTGACACTTCTTATTAGAAGTGTTGGTCGAATATTGAGTCGTTCTTATGGATTTTTCTGCCGCTTGAATCCCTTTCGGCAGCGTAGGATATTTGGAGGAGGAACGGTCGGTTCAAGGCCTGTAACGAGCATCGGCACATGGAGGTGTCAGGCATGTACATTTGACAATTCTGGTTTATTAAGTGCGTGTGAAATGTGTGGGACAGATCGGCAGGATGGGGTGCTGCATTCTAACCAATTATCGAGTCAATTGGATTTGACTTGGGAGGAAATGCGTCGTCGGAGACTTGAAAGATTTGGTTGATGGTAGAATAGATTGTTCATTTTCTCAGGCCACCAGATATTTACTTTTAGAAGAGTAAATTGAAAGGCTACTAGATGTTGAAGTTGTTTACACCATCTTTCGGAAATGATGAAGTTGTTTACTAGACGTTGAAAAAATGAAGTGGTATGGAGCATGGAGCTTGTGTTTAGGAACATAGCAAATTTGATTGAACACAGATAGAAAAGTCTTTGTACACGGGGAAGGAATTAACATACTTTCTTTTCATCATTGGTTTTCTATATGACATCTCTTCATGTAGCTGCTTCTTGATAACGGTTGAATAGATTTCTAATTGAAGTAATTGTTACACGAATGGCATCTATGATTGTTGTTGCAAGTTTTTCTAATGTGAAGGTTAATAATCAACCAAAAACTGACATTGCTAATGAACGGTTTGTCCTTTAATTG

mRNA sequence

AACAGTTTGGTACCCTAGTTATATCTATATATTTTTTTTGAAACAATATTTTCTTGAGTTGCAATGCATGAGAATGCTGCTCTTTCCTCTTTGTATTATTGTTCCTACTTTAACCAACTTTTTATTGGATACTATAGGGAATAAGCACAGGCAGGAATGTTACCACTGCTAGCTTACCAAGTAGTGAGCCAATACTACAGGCTCCAAGGGAACCCGATCACTCTGGGGCTCCTTGCCTCTAATACTCTCATTCATCTAAGGCCCAATTTTCTTCATCATATCATCCCTTCCATAGATGAGGTCTGGTTTAATGCACATCTCATCTTCAAGCACAAGGACTTGAAGCGTTTTTTACTATCACCATTCTACCATGTGGGTGACCCTCACCTTGTCTACAACATGATATCTCTACTATGGAAAGGCACTCAGTTGGAAACATCAATGGGTAGCATCGAATTTGCTTCAATGGTTGGTGCATTACTCATAATGTCGCAGGGAATTACCTTGTTGCTCATCAAATCCTTACTGATATTCTTTAATTATGGGAGGCCTTACTACTATGAATATTCTGTTGGGTTTTCCGGTGTACTTTTTGCTATGAAAGTTGTTCTTGGTTCTGAAACTGACAGCTACACTTACGTTTATGGTCTAAGAGTACCATCAAGTCATGCTGCATGGTTGGAACTAATACTTATCCAAATGGTTTCACCTGGTGTCTCTTTCCTTGGTCATCTTGGTGGTATACTTGCTGGTCTTCTCTTTTTGCGTCTGAAGGGTACGAATTCAGGGTCGGATCCATTGACACTTCTTATTAGAAGTGTTGGTCGAATATTGAGTCGTTCTTATGGATTTTTCTGCCGCTTGAATCCCTTTCGGCAGCGTAGGATATTTGGAGGAGGAACGGTCGGTTCAAGGCCTGTAACGAGCATCGGCACATGGAGGTGTCAGGCATGTACATTTGACAATTCTGGTTTATTAAGTGCGTGTGAAATGTGTGGGACAGATCGGCAGGATGGGGTGCTGCATTCTAACCAATTATCGAGTCAATTGGATTTGACTTGGGAGGAAATGCGTCGTCGGAGACTTGAAAGATTTGGTTGATGGTAGAATAGATTGTTCATTTTCTCAGGCCACCAGATATTTACTTTTAGAAGAGTAAATTGAAAGGCTACTAGATGTTGAAGTTGTTTACACCATCTTTCGGAAATGATGAAGTTGTTTACTAGACGTTGAAAAAATGAAGTGGTATGGAGCATGGAGCTTGTGTTTAGGAACATAGCAAATTTGATTGAACACAGATAGAAAAGTCTTTGTACACGGGGAAGGAATTAACATACTTTCTTTTCATCATTGGTTTTCTATATGACATCTCTTCATGTAGCTGCTTCTTGATAACGGTTGAATAGATTTCTAATTGAAGTAATTGTTACACGAATGGCATCTATGATTGTTGTTGCAAGTTTTTCTAATGTGAAGGTTAATAATCAACCAAAAACTGACATTGCTAATGAACGGTTTGTCCTTTAATTG

Coding sequence (CDS)

ATGTTACCACTGCTAGCTTACCAAGTAGTGAGCCAATACTACAGGCTCCAAGGGAACCCGATCACTCTGGGGCTCCTTGCCTCTAATACTCTCATTCATCTAAGGCCCAATTTTCTTCATCATATCATCCCTTCCATAGATGAGGTCTGGTTTAATGCACATCTCATCTTCAAGCACAAGGACTTGAAGCGTTTTTTACTATCACCATTCTACCATGTGGGTGACCCTCACCTTGTCTACAACATGATATCTCTACTATGGAAAGGCACTCAGTTGGAAACATCAATGGGTAGCATCGAATTTGCTTCAATGGTTGGTGCATTACTCATAATGTCGCAGGGAATTACCTTGTTGCTCATCAAATCCTTACTGATATTCTTTAATTATGGGAGGCCTTACTACTATGAATATTCTGTTGGGTTTTCCGGTGTACTTTTTGCTATGAAAGTTGTTCTTGGTTCTGAAACTGACAGCTACACTTACGTTTATGGTCTAAGAGTACCATCAAGTCATGCTGCATGGTTGGAACTAATACTTATCCAAATGGTTTCACCTGGTGTCTCTTTCCTTGGTCATCTTGGTGGTATACTTGCTGGTCTTCTCTTTTTGCGTCTGAAGGGTACGAATTCAGGGTCGGATCCATTGACACTTCTTATTAGAAGTGTTGGTCGAATATTGAGTCGTTCTTATGGATTTTTCTGCCGCTTGAATCCCTTTCGGCAGCGTAGGATATTTGGAGGAGGAACGGTCGGTTCAAGGCCTGTAACGAGCATCGGCACATGGAGGTGTCAGGCATGTACATTTGACAATTCTGGTTTATTAAGTGCGTGTGAAATGTGTGGGACAGATCGGCAGGATGGGGTGCTGCATTCTAACCAATTATCGAGTCAATTGGATTTGACTTGGGAGGAAATGCGTCGTCGGAGACTTGAAAGATTTGGTTGA

Protein sequence

MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHKDLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLIKSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELILIQMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFRQRRIFGGGTVGSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQLDLTWEEMRRRRLERFG*

Homology

[Close]

BLAST of CSPI04G20320.3 vs. ExPASy Swiss-Prot
Match: Q8RXW0 (Rhomboid-like protein 14, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=RBL14 PE=2 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 2.2e-102
Identity = 193/320 (60.31%), Postives = 237/320 (74.06%), Query Frame = 0


Query: 1   MLPLLAYQVVSQYYRLQGN-PITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKH 60
           MLPLLA   V++YYRL    P+T  LLA+NTL++LRP F+  +IP I EVWFN HLIFKH
Sbjct: 14  MLPLLALSAVAEYYRLPWKPPVTASLLAANTLVYLRPAFIDPVIPHISEVWFNPHLIFKH 73

Query: 61  KDLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLL 120
           KDLKR  LS FYHV +PHLVYNM+SLLWKG +LETSMGS EFASMV  L+ MSQG+TLLL
Sbjct: 74  KDLKRLFLSAFYHVNEPHLVYNMMSLLWKGIKLETSMGSSEFASMVFTLIGMSQGVTLLL 133

Query: 121 IKSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELIL 180
            KSLL+ F+Y R YY EY+VGFSGVLFAMKVVL S+ + Y+ VYG+ VP+ +AAW ELIL
Sbjct: 134 AKSLLLLFDYDRAYYNEYAVGFSGVLFAMKVVLNSQAEDYSSVYGILVPTKYAAWAELIL 193

Query: 181 IQMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPF 240
           +QM  P  SFLGHLGGILAG+++L+LKG+ SGSDP+T+ +R V R+++    F   +   
Sbjct: 194 VQMFVPNASFLGHLGGILAGIIYLKLKGSYSGSDPVTMAVRGVSRLVTWPLRFLNGMVRS 253

Query: 241 RQRRIFGGGTV--GSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDG----VLHSNQ 300
           R+RRI G G V  G   +   G WRCQ+CT+DNSG LSACEMCG+ R  G    +     
Sbjct: 254 RRRRITGRGRVGRGQTGIAGPGIWRCQSCTYDNSGWLSACEMCGSGRARGNGWSLNQGPA 313

Query: 301 LSSQLDLTWEEMRRRRLERF 314
           LSS  DL  +E+RRRR+ERF
Sbjct: 314 LSSSNDLPLDELRRRRVERF 333

[Close]

BLAST of CSPI04G20320.3 vs. ExPASy Swiss-Prot
Match: Q8BHC7 (Rhomboid-related protein 4 OS=Mus musculus OX=10090 GN=Rhbdd1 PE=1 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 2.5e-26
Identity = 101/316 (31.96%), Postives = 150/316 (47.47%), Query Frame = 0


Query: 9   VVSQYYRLQGN---PITLGLLASNTLIHLRP-NFLHHIIPSIDEVWFNAHLIFKHKDLKR 68
           ++SQ +++  N   P+TL  LA N    L P   L+H   S+++        ++ KD +R
Sbjct: 15  LLSQVFQIGINNIPPVTLATLAVNVWFFLNPWKPLYHSCISVEK-------CYQQKDWQR 74

Query: 69  FLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLIKSLL 128
            LLSP +H  D HL +NM+S+LWKG +LE  +GS  FA ++    +++ G+  LL++  +
Sbjct: 75  LLLSPLHHGDDWHLYFNMVSMLWKGVKLERRLGSRWFAYVIATFSLLT-GVVYLLLQFTV 134

Query: 129 IFFNYGRPYYYEYSVGFSGVLFAMKVVLGSE-TDSYTYVYGLRVPSSHAAWLELILIQMV 188
                   +    +VGFSGVLFA+KV+        +  + G  VP+  A W EL+ I   
Sbjct: 135 AELLNQPDFKRNCAVGFSGVLFALKVLSNHYCPGGFVNILGFPVPNRFACWAELVAIHFC 194

Query: 189 SPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFRQRR 248
           +PG SF GHL GIL GL++ +         PL  ++ +   I     G   + N F    
Sbjct: 195 TPGTSFAGHLAGILVGLMYTQ--------GPLKKIMDTCAGIFISHAGPSGQQNHFNNAG 254

Query: 249 IFG--GGTVGSRPVTSIGTWR----CQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQ 308
             G        RPVT   T+R      A   +   L  A      DR  G   +  +   
Sbjct: 255 PSGYQNHYADGRPVTYDATYRNYDVYTAGLSEEEQLERALRASIWDR--GNTRNGPMPYG 312

Query: 309 LDLTWEEMRRRRLERF 314
             L  EEMRR+RL RF
Sbjct: 315 FRLPPEEMRRQRLHRF 312

[Close]

BLAST of CSPI04G20320.3 vs. ExPASy Swiss-Prot
Match: Q8TEB9 (Rhomboid-related protein 4 OS=Homo sapiens OX=9606 GN=RHBDD1 PE=1 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 6.2e-25
Identity = 81/234 (34.62%), Postives = 120/234 (51.28%), Query Frame = 0


Query: 20  PITLGLLASNTLIHLRPNF-LHHIIPSIDEVWFNAHLIFKHKDLKRFLLSPFYHVGDPHL 79
           P+TL  LA N    L P   L+    S+++        ++ KD +R LLSP +H  D HL
Sbjct: 29  PVTLATLALNIWFFLNPQKPLYSSCLSVEK-------CYQQKDWQRLLLSPLHHADDWHL 88

Query: 80  VYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLIKSLLIFFNYGRPYYYEYS 139
            +NM S+LWKG  LE  +GS  FA ++ A  +++ G+  LL++  +  F     +    +
Sbjct: 89  YFNMASMLWKGINLERRLGSRWFAYVITAFSVLT-GVVYLLLQFAVAEFMDEPDFKRSCA 148

Query: 140 VGFSGVLFAMKVVLGSE-TDSYTYVYGLRVPSSHAAWLELILIQMVSPGVSFLGHLGGIL 199
           VGFSGVLFA+KV+        +  + G  VP+  A W+EL+ I + SPG SF GHL GIL
Sbjct: 149 VGFSGVLFALKVLNNHYCPGGFVNILGFPVPNRFACWVELVAIHLFSPGTSFAGHLAGIL 208

Query: 200 AGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFRQRRIFGGGTVG 252
            GL++ +         PL  ++ +     S S G+     P RQ      G+ G
Sbjct: 209 VGLMYTQ--------GPLKKIMEACAGGFSSSVGY-----PGRQYYFNSSGSSG 241

[Close]

BLAST of CSPI04G20320.3 vs. ExPASy Swiss-Prot
Match: Q4V8F3 (Rhomboid-related protein 4 OS=Rattus norvegicus OX=10116 GN=Rhbdd1 PE=2 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 5.8e-23
Identity = 71/199 (35.68%), Postives = 107/199 (53.77%), Query Frame = 0


Query: 9   VVSQYYRLQGN---PITLGLLASNTLIHLRP-NFLHHIIPSIDEVWFNAHLIFKHKDLKR 68
           ++SQ + +  N   P+TL  LA N    L P   L+H   S+++        ++  D +R
Sbjct: 15  LLSQVFHIGINNIPPVTLATLAVNVWFFLNPWKPLYHSCISVEK-------CYQQNDWQR 74

Query: 69  FLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLIKSLL 128
            LLSP +H  D HL +NM+S+LWKG +LE  +GS  FA ++    +++ G+  LL++   
Sbjct: 75  LLLSPVHHGDDWHLYFNMVSMLWKGVKLEKRLGSRWFAYIIATFSLLT-GVVYLLLQFAS 134

Query: 129 IFFNYGRPYYYEYSVGFSGVLFAMKVVLGSE-TDSYTYVYGLRVPSSHAAWLELILIQMV 188
                   +    +VGFSGVLFA+KV+        +  + G  VP+  A W EL  I   
Sbjct: 135 AELMNQPDFKRNCAVGFSGVLFALKVLSNHYCPGGFVNILGFPVPNRFACWAELAAIHFC 194

Query: 189 SPGVSFLGHLGGILAGLLF 203
           +PG SF GHL GIL GL++
Sbjct: 195 TPGTSFAGHLAGILVGLMY 205

[Close]

BLAST of CSPI04G20320.3 vs. ExPASy Swiss-Prot
Match: Q5RBS4 (Rhomboid-related protein 4 OS=Pongo abelii OX=9601 GN=RHBDD1 PE=2 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 2.9e-22
Identity = 67/185 (36.22%), Postives = 101/185 (54.59%), Query Frame = 0


Query: 20  PITLGLLASNTLIHLRPNF-LHHIIPSIDEVWFNAHLIFKHKDLKRFLLSPFYHVGDPHL 79
           P+TL  LA N    L P   L+    S+++        ++ +D +R LLSP +H  D HL
Sbjct: 29  PVTLATLALNIWFFLNPQKPLYSSCLSVEK-------CYQQRDWQRLLLSPLHHADDWHL 88

Query: 80  VYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLIKSLLIFFNYGRPYYYEYS 139
            +N  S+LWKG  LE  +GS  FA ++    +++ G+  LL++  +  F     +    +
Sbjct: 89  YFNTASVLWKGINLERRLGSRWFAYVITTFSVLT-GVVYLLLQFAVAEFMDEPDFKRSCA 148

Query: 140 VGFSGVLFAMKVVLGSE-TDSYTYVYGLRVPSSHAAWLELILIQMVSPGVSFLGHLGGIL 199
           VGFSGVLFA+KV+        +  + G  VP+  A W+EL+ I + SPG SF GH  GIL
Sbjct: 149 VGFSGVLFALKVLNNHYCPGGFVNILGFPVPNRFACWVELVAIHLFSPGTSFAGHQAGIL 205

Query: 200 AGLLF 203
            GL++
Sbjct: 209 VGLMY 205

[Close]

BLAST of CSPI04G20320.3 vs. ExPASy TrEMBL
Match: A0A0A0L173 (RanBP2-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G506850 PE=3 SV=1)

HSP 1 Score: 630.2 bits (1624), Expect = 4.7e-177
Identity = 313/314 (99.68%), Postives = 314/314 (100.00%), Query Frame = 0


Query: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK 60
           MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK
Sbjct: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK 60

Query: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI 120
           DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI
Sbjct: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI 120

Query: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELILI 180
           KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYV+GLRVPSSHAAWLELILI
Sbjct: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVHGLRVPSSHAAWLELILI 180

Query: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR 240
           QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR
Sbjct: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR 240

Query: 241 QRRIFGGGTVGSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQLDL 300
           QRRIFGGGTVGSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQLDL
Sbjct: 241 QRRIFGGGTVGSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQLDL 300

Query: 301 TWEEMRRRRLERFG 315
           TWEEMRRRRLERFG
Sbjct: 301 TWEEMRRRRLERFG 314

[Close]

BLAST of CSPI04G20320.3 vs. ExPASy TrEMBL
Match: A0A5A7SVC3 (Rhomboid-like protein 14 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold83G001640 PE=3 SV=1)

HSP 1 Score: 605.5 bits (1560), Expect = 1.2e-169
Identity = 301/314 (95.86%), Postives = 307/314 (97.77%), Query Frame = 0


Query: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK 60
           MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLI KHK
Sbjct: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLILKHK 60

Query: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI 120
           DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALL+MSQGITLLLI
Sbjct: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLVMSQGITLLLI 120

Query: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELILI 180
           KSLLIFFNYGRPYYYEYSVGFSGVLFA+KVVLGSET+SYTY++GLRVPSSHAAWLELILI
Sbjct: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFALKVVLGSETESYTYIHGLRVPSSHAAWLELILI 180

Query: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR 240
           QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSR  GFF RLNPFR
Sbjct: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRPLGFFSRLNPFR 240

Query: 241 QRRIFGGGTVGSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQLDL 300
           QRRIFGGGTVGSRPVTSIGTWRC+ACTFDNSGLLS CEMCGTDRQDGVLHSNQLSSQLD 
Sbjct: 241 QRRIFGGGTVGSRPVTSIGTWRCRACTFDNSGLLSTCEMCGTDRQDGVLHSNQLSSQLDF 300

Query: 301 TWEEMRRRRLERFG 315
           T EEMRRRRLERFG
Sbjct: 301 TREEMRRRRLERFG 314

[Close]

BLAST of CSPI04G20320.3 vs. ExPASy TrEMBL
Match: A0A1S3B4I1 (rhomboid-like protein 14, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103486069 PE=3 SV=1)

HSP 1 Score: 605.5 bits (1560), Expect = 1.2e-169
Identity = 301/314 (95.86%), Postives = 307/314 (97.77%), Query Frame = 0


Query: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK 60
           MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLI KHK
Sbjct: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLILKHK 60

Query: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI 120
           DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALL+MSQGITLLLI
Sbjct: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLVMSQGITLLLI 120

Query: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELILI 180
           KSLLIFFNYGRPYYYEYSVGFSGVLFA+KVVLGSET+SYTY++GLRVPSSHAAWLELILI
Sbjct: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFALKVVLGSETESYTYIHGLRVPSSHAAWLELILI 180

Query: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR 240
           QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSR  GFF RLNPFR
Sbjct: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRPLGFFSRLNPFR 240

Query: 241 QRRIFGGGTVGSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQLDL 300
           QRRIFGGGTVGSRPVTSIGTWRC+ACTFDNSGLLS CEMCGTDRQDGVLHSNQLSSQLD 
Sbjct: 241 QRRIFGGGTVGSRPVTSIGTWRCRACTFDNSGLLSTCEMCGTDRQDGVLHSNQLSSQLDF 300

Query: 301 TWEEMRRRRLERFG 315
           T EEMRRRRLERFG
Sbjct: 301 TREEMRRRRLERFG 314

[Close]

BLAST of CSPI04G20320.3 vs. ExPASy TrEMBL
Match: A0A6J1HXX5 (rhomboid-like protein 14, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111467237 PE=3 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 7.1e-141
Identity = 256/314 (81.53%), Postives = 281/314 (89.49%), Query Frame = 0


Query: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK 60
           MLPLLAYQVV+QY RLQG PIT+GLLA+NTLIHLRP FL  +IPSID+VWFNA+LI K+K
Sbjct: 1   MLPLLAYQVVTQYNRLQGKPITVGLLATNTLIHLRPGFLRSVIPSIDDVWFNAYLILKYK 60

Query: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI 120
           DLKRF LSPFYHVGD HL YNMISLLWKG QLE+SMGS +FASMVGALL+MSQGITLLL+
Sbjct: 61  DLKRFFLSPFYHVGDSHLAYNMISLLWKGIQLESSMGSTKFASMVGALLVMSQGITLLLV 120

Query: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELILI 180
           KSLL FFNY RPYY+EYSVGFSGVLFAMKVVLGS+++SYTYV+G+RVPSSHAAWLELILI
Sbjct: 121 KSLL-FFNYERPYYHEYSVGFSGVLFAMKVVLGSQSESYTYVHGVRVPSSHAAWLELILI 180

Query: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR 240
           Q+VSPGVSFLGHLGGILAG+LFLRLKG NSGSDPL +LIRSVGRILSR  GFFCRLNPFR
Sbjct: 181 QIVSPGVSFLGHLGGILAGILFLRLKGMNSGSDPLAVLIRSVGRILSRPLGFFCRLNPFR 240

Query: 241 QRRIFGGGTVGSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQLDL 300
             RIFGGGTVG R  TS+ TWRCQACTFDNS LL  CEMCGTDRQD  + S +LS + DL
Sbjct: 241 HHRIFGGGTVGRRQ-TSVDTWRCQACTFDNSCLLPRCEMCGTDRQDRAMPSRRLSHRFDL 300

Query: 301 TWEEMRRRRLERFG 315
           T EE+RRRRLERFG
Sbjct: 301 TPEELRRRRLERFG 312

[Close]

BLAST of CSPI04G20320.3 vs. ExPASy TrEMBL
Match: A0A6J1EL30 (rhomboid-like protein 14, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111435540 PE=3 SV=1)

HSP 1 Score: 506.9 bits (1304), Expect = 6.0e-140
Identity = 254/314 (80.89%), Postives = 281/314 (89.49%), Query Frame = 0


Query: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK 60
           MLPLLAYQVV+QY RLQG PIT+GLLA+NTLIHLRP FL  +IPSID+VWFNA+LI K+K
Sbjct: 1   MLPLLAYQVVTQYNRLQGKPITVGLLATNTLIHLRPGFLRSVIPSIDDVWFNAYLILKYK 60

Query: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI 120
           DLKRF LSPFYHVGD HL YNMISLLWKG QLE+SMGS +FASMVGALL+MSQGITLLL+
Sbjct: 61  DLKRFFLSPFYHVGDSHLAYNMISLLWKGIQLESSMGSTKFASMVGALLVMSQGITLLLV 120

Query: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELILI 180
           KSLL FFNY +PYY+EYSVGFSGVLFAMKVVLGS+++SYTYV+G+RVPSSHAAWLELILI
Sbjct: 121 KSLL-FFNYEKPYYHEYSVGFSGVLFAMKVVLGSQSESYTYVHGVRVPSSHAAWLELILI 180

Query: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR 240
           Q+VSPGVSFLGHLGGILAG+LFLRLKG N GSDPL +LIRSVGRILSR  GFFCRLNPFR
Sbjct: 181 QIVSPGVSFLGHLGGILAGILFLRLKGMNLGSDPLAVLIRSVGRILSRPLGFFCRLNPFR 240

Query: 241 QRRIFGGGTVGSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQLDL 300
            RRIFGGGTVG R  TS+ TWRC+ACTFDNS LL  CEMCGTDRQD  + S +LS + DL
Sbjct: 241 HRRIFGGGTVGRRQ-TSVDTWRCRACTFDNSCLLPRCEMCGTDRQDRAMPSRRLSHRFDL 300

Query: 301 TWEEMRRRRLERFG 315
           T EE+RRRRLERFG
Sbjct: 301 TPEELRRRRLERFG 312

[Close]

BLAST of CSPI04G20320.3 vs. NCBI nr
Match: XP_004146389.1 (rhomboid-like protein 14, mitochondrial [Cucumis sativus] >XP_011653901.1 rhomboid-like protein 14, mitochondrial [Cucumis sativus] >KGN54804.1 hypothetical protein Csa_012505 [Cucumis sativus])

HSP 1 Score: 630.2 bits (1624), Expect = 9.8e-177
Identity = 313/314 (99.68%), Postives = 314/314 (100.00%), Query Frame = 0


Query: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK 60
           MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK
Sbjct: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK 60

Query: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI 120
           DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI
Sbjct: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI 120

Query: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELILI 180
           KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYV+GLRVPSSHAAWLELILI
Sbjct: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVHGLRVPSSHAAWLELILI 180

Query: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR 240
           QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR
Sbjct: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR 240

Query: 241 QRRIFGGGTVGSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQLDL 300
           QRRIFGGGTVGSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQLDL
Sbjct: 241 QRRIFGGGTVGSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQLDL 300

Query: 301 TWEEMRRRRLERFG 315
           TWEEMRRRRLERFG
Sbjct: 301 TWEEMRRRRLERFG 314

[Close]

BLAST of CSPI04G20320.3 vs. NCBI nr
Match: XP_008442110.1 (PREDICTED: rhomboid-like protein 14, mitochondrial [Cucumis melo] >KAA0034920.1 rhomboid-like protein 14 [Cucumis melo var. makuwa] >TYK05460.1 rhomboid-like protein 14 [Cucumis melo var. makuwa])

HSP 1 Score: 605.5 bits (1560), Expect = 2.6e-169
Identity = 301/314 (95.86%), Postives = 307/314 (97.77%), Query Frame = 0


Query: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK 60
           MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLI KHK
Sbjct: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLILKHK 60

Query: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI 120
           DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALL+MSQGITLLLI
Sbjct: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLVMSQGITLLLI 120

Query: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELILI 180
           KSLLIFFNYGRPYYYEYSVGFSGVLFA+KVVLGSET+SYTY++GLRVPSSHAAWLELILI
Sbjct: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFALKVVLGSETESYTYIHGLRVPSSHAAWLELILI 180

Query: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR 240
           QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSR  GFF RLNPFR
Sbjct: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRPLGFFSRLNPFR 240

Query: 241 QRRIFGGGTVGSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQLDL 300
           QRRIFGGGTVGSRPVTSIGTWRC+ACTFDNSGLLS CEMCGTDRQDGVLHSNQLSSQLD 
Sbjct: 241 QRRIFGGGTVGSRPVTSIGTWRCRACTFDNSGLLSTCEMCGTDRQDGVLHSNQLSSQLDF 300

Query: 301 TWEEMRRRRLERFG 315
           T EEMRRRRLERFG
Sbjct: 301 TREEMRRRRLERFG 314

[Close]

BLAST of CSPI04G20320.3 vs. NCBI nr
Match: XP_038881196.1 (rhomboid-like protein 14, mitochondrial [Benincasa hispida])

HSP 1 Score: 553.9 bits (1426), Expect = 8.9e-154
Identity = 278/314 (88.54%), Postives = 291/314 (92.68%), Query Frame = 0


Query: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK 60
           MLPLLAYQVVSQYYRLQ NPIT+GLLA+NTLIHLRPNFL HIIPSID+VWFNAHLI KHK
Sbjct: 1   MLPLLAYQVVSQYYRLQRNPITVGLLATNTLIHLRPNFLRHIIPSIDDVWFNAHLILKHK 60

Query: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI 120
           DLKRFLLSPFYHVGDPHLVYNMISLLWKG QLETSMGSIEFASMV ALL+MSQGITLLLI
Sbjct: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGIQLETSMGSIEFASMVSALLVMSQGITLLLI 120

Query: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELILI 180
           KSLL+ FNY RPYYYEYSVGFSGVLFAMKVVLGS+ +SYTY++G+RVPSSHAAWLELILI
Sbjct: 121 KSLLV-FNYDRPYYYEYSVGFSGVLFAMKVVLGSQAESYTYLHGIRVPSSHAAWLELILI 180

Query: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR 240
           QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSD LTLLIR  G+ILSR  GFFCRLNPFR
Sbjct: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDLLTLLIRRAGQILSRPLGFFCRLNPFR 240

Query: 241 QRRIFGGGTVGSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQLDL 300
           QRRIFGGGTVGSRP TSI TWRCQACTFDNSGLLS CEMCGTDRQD VL  + LS Q DL
Sbjct: 241 QRRIFGGGTVGSRPATSIDTWRCQACTFDNSGLLSICEMCGTDRQDRVLPLHHLSRQFDL 300

Query: 301 TWEEMRRRRLERFG 315
           T EE+RRRRLERFG
Sbjct: 301 TREEVRRRRLERFG 313

[Close]

BLAST of CSPI04G20320.3 vs. NCBI nr
Match: XP_022967849.1 (rhomboid-like protein 14, mitochondrial [Cucurbita maxima] >XP_022967850.1 rhomboid-like protein 14, mitochondrial [Cucurbita maxima] >XP_022967852.1 rhomboid-like protein 14, mitochondrial [Cucurbita maxima] >XP_022967853.1 rhomboid-like protein 14, mitochondrial [Cucurbita maxima] >XP_022967854.1 rhomboid-like protein 14, mitochondrial [Cucurbita maxima])

HSP 1 Score: 510.0 bits (1312), Expect = 1.5e-140
Identity = 256/314 (81.53%), Postives = 281/314 (89.49%), Query Frame = 0


Query: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK 60
           MLPLLAYQVV+QY RLQG PIT+GLLA+NTLIHLRP FL  +IPSID+VWFNA+LI K+K
Sbjct: 1   MLPLLAYQVVTQYNRLQGKPITVGLLATNTLIHLRPGFLRSVIPSIDDVWFNAYLILKYK 60

Query: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI 120
           DLKRF LSPFYHVGD HL YNMISLLWKG QLE+SMGS +FASMVGALL+MSQGITLLL+
Sbjct: 61  DLKRFFLSPFYHVGDSHLAYNMISLLWKGIQLESSMGSTKFASMVGALLVMSQGITLLLV 120

Query: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELILI 180
           KSLL FFNY RPYY+EYSVGFSGVLFAMKVVLGS+++SYTYV+G+RVPSSHAAWLELILI
Sbjct: 121 KSLL-FFNYERPYYHEYSVGFSGVLFAMKVVLGSQSESYTYVHGVRVPSSHAAWLELILI 180

Query: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR 240
           Q+VSPGVSFLGHLGGILAG+LFLRLKG NSGSDPL +LIRSVGRILSR  GFFCRLNPFR
Sbjct: 181 QIVSPGVSFLGHLGGILAGILFLRLKGMNSGSDPLAVLIRSVGRILSRPLGFFCRLNPFR 240

Query: 241 QRRIFGGGTVGSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQLDL 300
             RIFGGGTVG R  TS+ TWRCQACTFDNS LL  CEMCGTDRQD  + S +LS + DL
Sbjct: 241 HHRIFGGGTVGRRQ-TSVDTWRCQACTFDNSCLLPRCEMCGTDRQDRAMPSRRLSHRFDL 300

Query: 301 TWEEMRRRRLERFG 315
           T EE+RRRRLERFG
Sbjct: 301 TPEELRRRRLERFG 312

[Close]

BLAST of CSPI04G20320.3 vs. NCBI nr
Match: KAG6603179.1 (Rhomboid-like protein 14, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033491.1 Rhomboid-like protein 14, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 507.7 bits (1306), Expect = 7.3e-140
Identity = 254/314 (80.89%), Postives = 281/314 (89.49%), Query Frame = 0


Query: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK 60
           MLPLLAYQVV+QY RLQG PIT+GLLA+NTLIHLRP FL  +IPSID+VWFNA+LI K+K
Sbjct: 1   MLPLLAYQVVTQYNRLQGKPITVGLLATNTLIHLRPGFLRSVIPSIDDVWFNAYLILKYK 60

Query: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI 120
           DLKRF LSPFYHVGD HL YNMISLLWKG QLE+SMGS +FASMVG LL+MSQGITLLL+
Sbjct: 61  DLKRFFLSPFYHVGDSHLAYNMISLLWKGIQLESSMGSTKFASMVGVLLVMSQGITLLLV 120

Query: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELILI 180
           KSLL FFNY +PYY+EYSVGFSGVLFAMKVVLGS+++SYTYV+G+RVPSSHAAWLELILI
Sbjct: 121 KSLL-FFNYEKPYYHEYSVGFSGVLFAMKVVLGSQSESYTYVHGVRVPSSHAAWLELILI 180

Query: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR 240
           Q+VSPGVSFLGHLGGILAG+LFLRLKG NSGSDPL +LIRSVGRILSR  GFFCRLNPFR
Sbjct: 181 QIVSPGVSFLGHLGGILAGILFLRLKGMNSGSDPLAVLIRSVGRILSRPLGFFCRLNPFR 240

Query: 241 QRRIFGGGTVGSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQLDL 300
            RRIFGGGTVG R  TS+ TWRC+ACTFDNS LL  CEMCGTDRQD  + S +LS + DL
Sbjct: 241 HRRIFGGGTVGRRQ-TSVDTWRCRACTFDNSCLLPRCEMCGTDRQDRAMPSRRLSHRFDL 300

Query: 301 TWEEMRRRRLERFG 315
           T EE+RRRRLERFG
Sbjct: 301 TPEELRRRRLERFG 312

[Close]

BLAST of CSPI04G20320.3 vs. TAIR 10
Match: AT3G17611.1 (RHOMBOID-like protein 14 )

HSP 1 Score: 373.6 bits (958), Expect = 1.5e-103
Identity = 193/320 (60.31%), Postives = 237/320 (74.06%), Query Frame = 0


Query: 1   MLPLLAYQVVSQYYRLQGN-PITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKH 60
           MLPLLA   V++YYRL    P+T  LLA+NTL++LRP F+  +IP I EVWFN HLIFKH
Sbjct: 14  MLPLLALSAVAEYYRLPWKPPVTASLLAANTLVYLRPAFIDPVIPHISEVWFNPHLIFKH 73

Query: 61  KDLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLL 120
           KDLKR  LS FYHV +PHLVYNM+SLLWKG +LETSMGS EFASMV  L+ MSQG+TLLL
Sbjct: 74  KDLKRLFLSAFYHVNEPHLVYNMMSLLWKGIKLETSMGSSEFASMVFTLIGMSQGVTLLL 133

Query: 121 IKSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELIL 180
            KSLL+ F+Y R YY EY+VGFSGVLFAMKVVL S+ + Y+ VYG+ VP+ +AAW ELIL
Sbjct: 134 AKSLLLLFDYDRAYYNEYAVGFSGVLFAMKVVLNSQAEDYSSVYGILVPTKYAAWAELIL 193

Query: 181 IQMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPF 240
           +QM  P  SFLGHLGGILAG+++L+LKG+ SGSDP+T+ +R V R+++    F   +   
Sbjct: 194 VQMFVPNASFLGHLGGILAGIIYLKLKGSYSGSDPVTMAVRGVSRLVTWPLRFLNGMVRS 253

Query: 241 RQRRIFGGGTV--GSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDG----VLHSNQ 300
           R+RRI G G V  G   +   G WRCQ+CT+DNSG LSACEMCG+ R  G    +     
Sbjct: 254 RRRRITGRGRVGRGQTGIAGPGIWRCQSCTYDNSGWLSACEMCGSGRARGNGWSLNQGPA 313

Query: 301 LSSQLDLTWEEMRRRRLERF 314
           LSS  DL  +E+RRRR+ERF
Sbjct: 314 LSSSNDLPLDELRRRRVERF 333

[Close]

BLAST of CSPI04G20320.3 vs. TAIR 10
Match: AT3G17611.2 (RHOMBOID-like protein 14 )

HSP 1 Score: 263.8 bits (673), Expect = 1.7e-70
Identity = 139/238 (58.40%), Postives = 174/238 (73.11%), Query Frame = 0


Query: 82  MISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLIKSLLIFFNYGRPYYYEYSVGF 141
           M+SLLWKG +LETSMGS EFASMV  L+ MSQG+TLLL KSLL+ F+Y R YY EY+VGF
Sbjct: 1   MMSLLWKGIKLETSMGSSEFASMVFTLIGMSQGVTLLLAKSLLLLFDYDRAYYNEYAVGF 60

Query: 142 SGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELILIQMVSPGVSFLGHLGGILAGLL 201
           SGVLFAMKVVL S+ + Y+ VYG+ VP+ +AAW ELIL+QM  P  SFLGHLGGILAG++
Sbjct: 61  SGVLFAMKVVLNSQAEDYSSVYGILVPTKYAAWAELILVQMFVPNASFLGHLGGILAGII 120

Query: 202 FLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFRQRRIFGGGTV--GSRPVTSIG 261
           +L+LKG+ SGSDP+T+ +R V R+++    F   +   R+RRI G G V  G   +   G
Sbjct: 121 YLKLKGSYSGSDPVTMAVRGVSRLVTWPLRFLNGMVRSRRRRITGRGRVGRGQTGIAGPG 180

Query: 262 TWRCQACTFDNSGLLSACEMCGTDRQDG----VLHSNQLSSQLDLTWEEMRRRRLERF 314
            WRCQ+CT+DNSG LSACEMCG+ R  G    +     LSS  DL  +E+RRRR+ERF
Sbjct: 181 IWRCQSCTYDNSGWLSACEMCGSGRARGNGWSLNQGPALSSSNDLPLDELRRRRVERF 238

[Close]

BLAST of CSPI04G20320.3 vs. TAIR 10
Match: AT3G17611.3 (RHOMBOID-like protein 14 )

HSP 1 Score: 174.9 bits (442), Expect = 1.0e-43
Identity = 90/172 (52.33%), Postives = 118/172 (68.60%), Query Frame = 0


Query: 148 MKVVLGSETDSYTYVYGLRVPSSHAAWLELILIQMVSPGVSFLGHLGGILAGLLFLRLKG 207
           MKVVL S+ + Y+ VYG+ VP+ +AAW ELIL+QM  P  SFLGHLGGILAG+++L+LKG
Sbjct: 1   MKVVLNSQAEDYSSVYGILVPTKYAAWAELILVQMFVPNASFLGHLGGILAGIIYLKLKG 60

Query: 208 TNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFRQRRIFGGGTV--GSRPVTSIGTWRCQA 267
           + SGSDP+T+ +R V R+++    F   +   R+RRI G G V  G   +   G WRCQ+
Sbjct: 61  SYSGSDPVTMAVRGVSRLVTWPLRFLNGMVRSRRRRITGRGRVGRGQTGIAGPGIWRCQS 120

Query: 268 CTFDNSGLLSACEMCGTDRQDG----VLHSNQLSSQLDLTWEEMRRRRLERF 314
           CT+DNSG LSACEMCG+ R  G    +     LSS  DL  +E+RRRR+ERF
Sbjct: 121 CTYDNSGWLSACEMCGSGRARGNGWSLNQGPALSSSNDLPLDELRRRRVERF 172

[Close]

BLAST of CSPI04G20320.3 vs. TAIR 10
Match: AT3G58460.1 (RHOMBOID-like protein 15 )

HSP 1 Score: 73.2 bits (178), Expect = 4.3e-13
Identity = 55/162 (33.95%), Postives = 87/162 (53.70%), Query Frame = 0


Query: 48  EVWFNAHLIFKHKDLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGA 107
           EV F    I     + RF  +  +H    H+++NM++L+  G++LE  MGS+    +   
Sbjct: 50  EVCFLPSAIISRFQVYRFYTAIIFHGSLLHVLFNMMALVPMGSELERIMGSVRLLYLT-V 109

Query: 108 LLIMSQGITLLLIKSLLIFFNYGRPYYYEY-----SVGFSGVLFAMKVVLGSETD-SYTY 167
           LL  +  +  LLI SL     Y   Y Y++     ++GFSG+LF+M V+  S +  +   
Sbjct: 110 LLATTNAVLHLLIASLA---GYNPFYQYDHLMNECAIGFSGILFSMIVIETSLSGVTSRS 169

Query: 168 VYGL-RVPSSHAAWLELILIQMVSPGVSFLGHLGGILAGLLF 203
           V+GL  VP+    W+ LI+ Q++   VS LGHL GIL+G  +
Sbjct: 170 VFGLFNVPAKLYPWILLIVFQLLMTNVSLLGHLCGILSGFSY 207

[Close]

BLAST of CSPI04G20320.3 vs. TAIR 10
Match: AT3G58460.2 (RHOMBOID-like protein 15 )

HSP 1 Score: 73.2 bits (178), Expect = 4.3e-13
Identity = 55/162 (33.95%), Postives = 87/162 (53.70%), Query Frame = 0


Query: 48  EVWFNAHLIFKHKDLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGA 107
           EV F    I     + RF  +  +H    H+++NM++L+  G++LE  MGS+    +   
Sbjct: 73  EVCFLPSAIISRFQVYRFYTAIIFHGSLLHVLFNMMALVPMGSELERIMGSVRLLYLT-V 132

Query: 108 LLIMSQGITLLLIKSLLIFFNYGRPYYYEY-----SVGFSGVLFAMKVVLGSETD-SYTY 167
           LL  +  +  LLI SL     Y   Y Y++     ++GFSG+LF+M V+  S +  +   
Sbjct: 133 LLATTNAVLHLLIASLA---GYNPFYQYDHLMNECAIGFSGILFSMIVIETSLSGVTSRS 192

Query: 168 VYGL-RVPSSHAAWLELILIQMVSPGVSFLGHLGGILAGLLF 203
           V+GL  VP+    W+ LI+ Q++   VS LGHL GIL+G  +
Sbjct: 193 VFGLFNVPAKLYPWILLIVFQLLMTNVSLLGHLCGILSGFSY 230

The following BLAST results are available for this feature:

BLAST of CSPI04G20320.3 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RXW0	2.2e-102	60.31	Rhomboid-like protein 14, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=RBL14...	[more]
Q8BHC7	2.5e-26	31.96	Rhomboid-related protein 4 OS=Mus musculus OX=10090 GN=Rhbdd1 PE=1 SV=1	[more]
Q8TEB9	6.2e-25	34.62	Rhomboid-related protein 4 OS=Homo sapiens OX=9606 GN=RHBDD1 PE=1 SV=1	[more]
Q4V8F3	5.8e-23	35.68	Rhomboid-related protein 4 OS=Rattus norvegicus OX=10116 GN=Rhbdd1 PE=2 SV=1	[more]
Q5RBS4	2.9e-22	36.22	Rhomboid-related protein 4 OS=Pongo abelii OX=9601 GN=RHBDD1 PE=2 SV=1	[more]

BLAST of CSPI04G20320.3 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L173	4.7e-177	99.68	RanBP2-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G506850...	[more]
A0A5A7SVC3	1.2e-169	95.86	Rhomboid-like protein 14 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A1S3B4I1	1.2e-169	95.86	rhomboid-like protein 14, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103486069 ...	[more]
A0A6J1HXX5	7.1e-141	81.53	rhomboid-like protein 14, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111467...	[more]
A0A6J1EL30	6.0e-140	80.89	rhomboid-like protein 14, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]

BLAST of CSPI04G20320.3 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004146389.1	9.8e-177	99.68	rhomboid-like protein 14, mitochondrial [Cucumis sativus] >XP_011653901.1 rhombo...	[more]
XP_008442110.1	2.6e-169	95.86	PREDICTED: rhomboid-like protein 14, mitochondrial [Cucumis melo] >KAA0034920.1 ...	[more]
XP_038881196.1	8.9e-154	88.54	rhomboid-like protein 14, mitochondrial [Benincasa hispida]	[more]
XP_022967849.1	1.5e-140	81.53	rhomboid-like protein 14, mitochondrial [Cucurbita maxima] >XP_022967850.1 rhomb...	[more]
KAG6603179.1	7.3e-140	80.89	Rhomboid-like protein 14, mitochondrial, partial [Cucurbita argyrosperma subsp. ...	[more]

BLAST of CSPI04G20320.3 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G17611.1	1.5e-103	60.31	RHOMBOID-like protein 14	[more]
AT3G17611.2	1.7e-70	58.40	RHOMBOID-like protein 14	[more]
AT3G17611.3	1.0e-43	52.33	RHOMBOID-like protein 14	[more]
AT3G58460.1	4.3e-13	33.95	RHOMBOID-like protein 15	[more]
AT3G58460.2	4.3e-13	33.95	RHOMBOID-like protein 15	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001876	Zinc finger, RanBP2-type	SMART	SM00547	zf_4	coord: 259..283 e-value: 4.5E-4 score: 29.5
IPR001876	Zinc finger, RanBP2-type	PFAM	PF00641	zf-RanBP	coord: 258..286 e-value: 5.5E-7 score: 28.8
IPR001876	Zinc finger, RanBP2-type	PROSITE	PS01358	ZF_RANBP2_1	coord: 261..280
IPR001876	Zinc finger, RanBP2-type	PROSITE	PS50199	ZF_RANBP2_2	coord: 257..286 score: 8.738674
IPR022764	Peptidase S54, rhomboid domain	PFAM	PF01694	Rhomboid	coord: 58..204 e-value: 2.1E-20 score: 73.3
IPR035952	Rhomboid-like superfamily	GENE3D	1.20.1540.10		coord: 12..209 e-value: 2.2E-22 score: 81.6
IPR035952	Rhomboid-like superfamily	SUPERFAMILY	144091	Rhomboid-like	coord: 19..204
None	No IPR available	GENE3D	2.30.30.380		coord: 254..312 e-value: 2.4E-8 score: 35.6
None	No IPR available	PANTHER	PTHR43066:SF1	RHOMBOID PROTEIN 2	coord: 1..312
None	No IPR available	PANTHER	PTHR43066	RHOMBOID-RELATED PROTEIN	coord: 1..312
IPR036443	Zinc finger, RanBP2-type superfamily	SUPERFAMILY	90209	Ran binding protein zinc finger-like	coord: 260..284

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI04G20320	CSPI04G20320	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI04G20320.3.utr5p1	CSPI04G20320.3.utr5p1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI04G20320.3.cds1	CSPI04G20320.3.cds1	CDS
CSPI04G20320.3.cds2	CSPI04G20320.3.cds2	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI04G20320.3.utr3p1	CSPI04G20320.3.utr3p1	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI04G20320.3	CSPI04G20320.3-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0004252	serine-type endopeptidase activity
molecular_function	GO:0046872	metal ion binding