CSPI04G20320 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI04G20320
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	RanBP2-type domain-containing protein
Location	Chr4: 18429919 .. 18433528 (+)
RNA-Seq Expression	CSPI04G20320
Synteny	CSPI04G20320

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGAAAAGAACATTGGTGAGTTTTCTAGAGATTTCGTAGTCGGCAAGGCAATCCAAGGTGGAGTTCTTAATCGGTGTGACATCGGAGTTATTTTGTACGTCGACACTCTTTCCCTTTGGTCAAATACTCTACTTTGGTGCTCCTTCGTTCTTTTCTGCTCTTTCAAGTTTCAACAGGTAAGATTCACGGTTAATTCTCAATTTCCTGCTTTCAAACCCCATTTCCTTGATTTCTTCACTCCTCGATTTATTTCTTTTCGCAAAATATGGACTATCTTTTGATAGAATCACGATTGTGTCATCAATTTAGTGAATTTGTTTTTTCCTCTGTTGCATGTTCTTGGTTTTGTGATTGTATAATGGGAACACTTGCTGCTCGCTCGTTGTTCAAATTATTGCTTTTTTTTAGGCTTTGAATACTCGAATGCCATTTCTCAATAGGTGTAGTTTTTATGTTTTGATCTTTAATGGTTGCGAGCTATGTGAGTTACCATAGATGATTTGTTTTGTGTTAAGCTATGTGGACTGCTTTAAAATTGAATTTATTTGGATTTGGAATGGTCTTGAATTTTGAGGATTTGGTACTGCTAATTATTTATAGGGGTTCCATTTTGGAAGTAGGACTATAGGTTTCCAGCCTAATATTTTGTATATCGTGTATGCACAACCTTGGCCGTTTCCTGTACATAATATAAAAATCACAACCAATAAAATGGCACATGTCAAAATCGACCTAATGACACGTCAGAGATTGGAGAGATATTTTTAGCAAGCAGTGGACGCAAGGCTAAAAGTCATCTTCAGCTATGCCAAACTTTTAATGCATTGCTATGGCAACCCAGTTCTTCCACTTGAACCTTAACTATAATTTGGTATTTATTTCTTCCTGAAATAGATACTACATTTATGTAAGAATAAGGGTTTGCTTCAGGAAGTTTGTGTGAGGACCCTAGATAGGAAAACAGCATAGAAAATGAAGATGGTTACTGGTGGGGCATGTTAGCGAATAGTTAGTAAGTTAGTTAGGTCTTTTTGTTATAAATAGTAGGAGAGTGGTTGAGGAGAGGTGTGAAGAATTTTGTGGAGGGATTTCCTTGTAAAGGGAATTTGGGAGAGTCCAGCCCTCTCGAAAGGTTAGGTTACTTTGTTGCTTGTCTTGTGGTATTCTAGAATATATTACATATATTTTCATATTTGATTGTTCTATAACTGTTCTTGAGCTCATCCTTTGTTCTTGAGATAGTTCTTGTTAGGAGGGATCTTAACAGTTTGGTACCCTAGTTATATCTATATATTTTTTTTGAAACAATATTTTCTTGAGTTGCAATGCATGAGAATGCTGCTCTTTCCTCTTTGTATTATTGTTCCTACTTTAACCAACTTTTTATTGGATACTATAGGGAATAAGCACAGGCAGGAATGTTACCACTGCTAGCTTACCAAGTAGTGAGCCAATACTACAGGCTCCAAGGGAACCCGATCACTCTGGGGCTCCTTGCCTCTAATACTCTCATTCATCTAAGGCCCAATTTTCTTCATCATATCATCCCTTCCATAGATGAGGTCTGGTTTAATGCACATCTCATCTTCAAGGTGACTATTCTGGCTAATAATCTAATTCTATTCTGCTGTTCTCTGTTTAAGACTTAACTGCACTCTCTATGTTAGAGTTAATTGGTGATGATTATAAAAATTTAATGAGAGATCTTTCTGCTGAGGACTTTCCTATTCATTAATACATCGGTAGGGAAGTCCTAAACAGAATGGCACGTTACTTTCTCTGGAATAAAGCATTGCTGTCTCATTGAGAAATTGGTATCATGTTTCTTTTTTGATCAGCTGTATAAATAATCTTGTAGTTTCCTTAGATACTGACTACCTGCTTTATAGCATCATTGAAGCGAATAGGTCTTTATTTATTTATTGAACTTTTTAGCTGTGAAGTCATTTTTCATTGAGAAAAATGGAAGAAATAAGTAGATACTAGTTGAATTTGTATGCTTTAACCATAAGAAATGGTTCTTGTATGATGTTGAACGTTACAAAACACATATAGATTGGGTAGCCCCCACATAACTGTGTAATTTGTGTCCTTACAAATCGTCCTCATAGTGAGGTTGTTGTATGATGGCCTCAAATTATAGTTAAACTTTTATTTTGAGAAAAATGAAAAGCTTTTAAGAGTTTGCTTTGATTTTTGTACAATATTCACAGTTTCTTTCTTTTCAGTATTGTACTTCTTTAATCCATCTTGATGAGTATATACCTAACAATTTTGAACTCCTGTAGGATTATTTTCTTTTTTGTTGTTTATTAACTTGTTTGCATGTCGTTTTTGAATGAGATAATGTGATATTGGCATTCCTGTTACTGTCATAATATTGGTATCATTTGCATTTCCCACTTTGCCAGCACAAGGACTTGAAGCGTTTTTTACTATCACCATTCTACCATGTGGGTGACCCTCACCTTGTCTACAACATGATATCTCTACTATGGAAAGGCACTCAGTTGGAAACATCAATGGGTAGCATCGAATTTGCTTCAATGGTTGGTGCATTACTCATAATGTCGCAGGGAATTACCTTGTTGCTCATCAAATCCTTACTGATATTCTTTAATTATGGGAGGCCTTACTACTATGAATATTCTGTTGGGTTTTCCGGTGTACTTTTTGCTATGAAAGTTGTTCTTGGTTCTGAAACTGACAGCTACACTTACGTTTATGGTCTAAGAGTACCATCAAGTCATGCTGCATGGTTGGAACTAATACTTATCCAAATGGTTTCACCTGGTGTCTCTTTCCTTGGTCATCTTGGTGGTATACTTGCTGGTCTTCTCTTTTTGCGTCTGAAGGGTACGAATTCAGGGTCGGATCCATTGACACTTCTTATTAGAAGTGTTGGTCGAATATTGAGTCGTTCTTATGGATTTTTCTGCCGCTTGAATCCCTTTCGGCAGCGTAGGATATTTGGAGGAGGAACGGTCGGTTCAAGGCCTGTAACGAGCATCGGCACATGGAGGTGTCAGGCATGTACATTTGACAATTCTGGTTTATTAAGTGCGTGTGAAATGTGTGGGACAGATCGGCAGGATGGGGTGCTGCATTCTAACCAATTATCGAGTCAATTGGATTTGACTTGGGAGGAAATGCGTCGTCGGAGACTTGAAAGATTTGGTTGATGGTAGAATAGATTGTTCATTTTCTCAGGCCACCAGATATTTACTTTTAGAAGAGTAAATTGAAAGGCTACTAGATGTTGAAGTTGTTTACACCATCTTTCGGAAATGATGAAGTTGTTTACTAGACGTTGAAAAAATGAAGTGGTATGGAGCATGGAGCTTGTGTTTAGGAACATAGCAAATTTGATTGAACACAGATAGAAAAGTCTTTGTACACGGGGAAGGAATTAACATACTTTCTTTTCATCATTGGTTTTCTATATGACATCTCTTCATGTAGCTGCTTCTTGATAACGGTTGAATAGATTTCTAATTGAAGTAATTGTTACACGAATGGCATCTATGATTGTTGTTGCAAGTTTTTCTAATGTGAAGGTTAATAATCAACCAAAAACTGACATTGCTAATGAACGGTTTGTCCTTTAATTG

mRNA sequence

GGAAAAGAACATTGGTGAGTTTTCTAGAGATTTCGTAGTCGGCAAGGCAATCCAAGGTGGAGTTCTTAATCGGTGTGACATCGGAGTTATTTTGTACGTCGACACTCTTTCCCTTTGGTCAAATACTCTACTTTGGTGCTCCTTCGTTCTTTTCTGCTCTTTCAAGTTTCAACAGGGAATAAGCACAGGCAGGAATGTTACCACTGCTAGCTTACCAAGTAGTGAGCCAATACTACAGGCTCCAAGGGAACCCGATCACTCTGGGGCTCCTTGCCTCTAATACTCTCATTCATCTAAGGCCCAATTTTCTTCATCATATCATCCCTTCCATAGATGAGGTCTGGTTTAATGCACATCTCATCTTCAAGCACAAGGACTTGAAGCGTTTTTTACTATCACCATTCTACCATGTGGGTGACCCTCACCTTGTCTACAACATGATATCTCTACTATGGAAAGGCACTCAGTTGGAAACATCAATGGGTAGCATCGAATTTGCTTCAATGGTTGGTGCATTACTCATAATGTCGCAGGGAATTACCTTGTTGCTCATCAAATCCTTACTGATATTCTTTAATTATGGGAGGCCTTACTACTATGAATATTCTGTTGGGTTTTCCGGTGTACTTTTTGCTATGAAAGTTGTTCTTGGTTCTGAAACTGACAGCTACACTTACGTTTATGGTCTAAGAGTACCATCAAGTCATGCTGCATGGTTGGAACTAATACTTATCCAAATGGTTTCACCTGGTGTCTCTTTCCTTGGTCATCTTGGTGGTATACTTGCTGGTCTTCTCTTTTTGCGTCTGAAGGGTACGAATTCAGGGTCGGATCCATTGACACTTCTTATTAGAAGTGTTGGTCGAATATTGAGTCGTTCTTATGGATTTTTCTGCCGCTTGAATCCCTTTCGGCAGCGTAGGATATTTGGAGGAGGAACGGTCGGTTCAAGGCCTGTAACGAGCATCGGCACATGGAGGTGTCAGGCATGTACATTTGACAATTCTGGTTTATTAAGTGCGTGTGAAATGTGTGGGACAGATCGGCAGGATGGGGTGCTGCATTCTAACCAATTATCGAGTCAATTGGATTTGACTTGGGAGGAAATGCGTCGTCGGAGACTTGAAAGATTTGGTTGATGGTAGAATAGATTGTTCATTTTCTCAGGCCACCAGATATTTACTTTTAGAAGAGTAAATTGAAAGGCTACTAGATGTTGAAGTTGTTTACACCATCTTTCGGAAATGATGAAGTTGTTTACTAGACGTTGAAAAAATGAAGTGGTATGGAGCATGGAGCTTGTGTTTAGGAACATAGCAAATTTGATTGAACACAGATAGAAAAGTCTTTGTACACGGGGAAGGAATTAACATACTTTCTTTTCATCATTGGTTTTCTATATGACATCTCTTCATGTAGCTGCTTCTTGATAACGGTTGAATAGATTTCTAATTGAAGTAATTGTTACACGAATGGCATCTATGATTGTTGTTGCAAGTTTTTCTAATGTGAAGGTTAATAATCAACCAAAAACTGACATTGCTAATGAACGGTTTGTCCTTTAATTG

Coding sequence (CDS)

ATGTTACCACTGCTAGCTTACCAAGTAGTGAGCCAATACTACAGGCTCCAAGGGAACCCGATCACTCTGGGGCTCCTTGCCTCTAATACTCTCATTCATCTAAGGCCCAATTTTCTTCATCATATCATCCCTTCCATAGATGAGGTCTGGTTTAATGCACATCTCATCTTCAAGCACAAGGACTTGAAGCGTTTTTTACTATCACCATTCTACCATGTGGGTGACCCTCACCTTGTCTACAACATGATATCTCTACTATGGAAAGGCACTCAGTTGGAAACATCAATGGGTAGCATCGAATTTGCTTCAATGGTTGGTGCATTACTCATAATGTCGCAGGGAATTACCTTGTTGCTCATCAAATCCTTACTGATATTCTTTAATTATGGGAGGCCTTACTACTATGAATATTCTGTTGGGTTTTCCGGTGTACTTTTTGCTATGAAAGTTGTTCTTGGTTCTGAAACTGACAGCTACACTTACGTTTATGGTCTAAGAGTACCATCAAGTCATGCTGCATGGTTGGAACTAATACTTATCCAAATGGTTTCACCTGGTGTCTCTTTCCTTGGTCATCTTGGTGGTATACTTGCTGGTCTTCTCTTTTTGCGTCTGAAGGGTACGAATTCAGGGTCGGATCCATTGACACTTCTTATTAGAAGTGTTGGTCGAATATTGAGTCGTTCTTATGGATTTTTCTGCCGCTTGAATCCCTTTCGGCAGCGTAGGATATTTGGAGGAGGAACGGTCGGTTCAAGGCCTGTAACGAGCATCGGCACATGGAGGTGTCAGGCATGTACATTTGACAATTCTGGTTTATTAAGTGCGTGTGAAATGTGTGGGACAGATCGGCAGGATGGGGTGCTGCATTCTAACCAATTATCGAGTCAATTGGATTTGACTTGGGAGGAAATGCGTCGTCGGAGACTTGAAAGATTTGGTTGA

Protein sequence

MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHKDLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLIKSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELILIQMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFRQRRIFGGGTVGSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQLDLTWEEMRRRRLERFG*

Homology

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BLAST of CSPI04G20320 vs. ExPASy Swiss-Prot
Match: Q8RXW0 (Rhomboid-like protein 14, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=RBL14 PE=2 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 2.2e-102
Identity = 193/320 (60.31%), Postives = 237/320 (74.06%), Query Frame = 0


Query: 1   MLPLLAYQVVSQYYRLQGN-PITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKH 60
           MLPLLA   V++YYRL    P+T  LLA+NTL++LRP F+  +IP I EVWFN HLIFKH
Sbjct: 14  MLPLLALSAVAEYYRLPWKPPVTASLLAANTLVYLRPAFIDPVIPHISEVWFNPHLIFKH 73

Query: 61  KDLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLL 120
           KDLKR  LS FYHV +PHLVYNM+SLLWKG +LETSMGS EFASMV  L+ MSQG+TLLL
Sbjct: 74  KDLKRLFLSAFYHVNEPHLVYNMMSLLWKGIKLETSMGSSEFASMVFTLIGMSQGVTLLL 133

Query: 121 IKSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELIL 180
            KSLL+ F+Y R YY EY+VGFSGVLFAMKVVL S+ + Y+ VYG+ VP+ +AAW ELIL
Sbjct: 134 AKSLLLLFDYDRAYYNEYAVGFSGVLFAMKVVLNSQAEDYSSVYGILVPTKYAAWAELIL 193

Query: 181 IQMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPF 240
           +QM  P  SFLGHLGGILAG+++L+LKG+ SGSDP+T+ +R V R+++    F   +   
Sbjct: 194 VQMFVPNASFLGHLGGILAGIIYLKLKGSYSGSDPVTMAVRGVSRLVTWPLRFLNGMVRS 253

Query: 241 RQRRIFGGGTV--GSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDG----VLHSNQ 300
           R+RRI G G V  G   +   G WRCQ+CT+DNSG LSACEMCG+ R  G    +     
Sbjct: 254 RRRRITGRGRVGRGQTGIAGPGIWRCQSCTYDNSGWLSACEMCGSGRARGNGWSLNQGPA 313

Query: 301 LSSQLDLTWEEMRRRRLERF 314
           LSS  DL  +E+RRRR+ERF
Sbjct: 314 LSSSNDLPLDELRRRRVERF 333

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BLAST of CSPI04G20320 vs. ExPASy Swiss-Prot
Match: Q8BHC7 (Rhomboid-related protein 4 OS=Mus musculus OX=10090 GN=Rhbdd1 PE=1 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 2.5e-26
Identity = 101/316 (31.96%), Postives = 150/316 (47.47%), Query Frame = 0


Query: 9   VVSQYYRLQGN---PITLGLLASNTLIHLRP-NFLHHIIPSIDEVWFNAHLIFKHKDLKR 68
           ++SQ +++  N   P+TL  LA N    L P   L+H   S+++        ++ KD +R
Sbjct: 15  LLSQVFQIGINNIPPVTLATLAVNVWFFLNPWKPLYHSCISVEK-------CYQQKDWQR 74

Query: 69  FLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLIKSLL 128
            LLSP +H  D HL +NM+S+LWKG +LE  +GS  FA ++    +++ G+  LL++  +
Sbjct: 75  LLLSPLHHGDDWHLYFNMVSMLWKGVKLERRLGSRWFAYVIATFSLLT-GVVYLLLQFTV 134

Query: 129 IFFNYGRPYYYEYSVGFSGVLFAMKVVLGSE-TDSYTYVYGLRVPSSHAAWLELILIQMV 188
                   +    +VGFSGVLFA+KV+        +  + G  VP+  A W EL+ I   
Sbjct: 135 AELLNQPDFKRNCAVGFSGVLFALKVLSNHYCPGGFVNILGFPVPNRFACWAELVAIHFC 194

Query: 189 SPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFRQRR 248
           +PG SF GHL GIL GL++ +         PL  ++ +   I     G   + N F    
Sbjct: 195 TPGTSFAGHLAGILVGLMYTQ--------GPLKKIMDTCAGIFISHAGPSGQQNHFNNAG 254

Query: 249 IFG--GGTVGSRPVTSIGTWR----CQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQ 308
             G        RPVT   T+R      A   +   L  A      DR  G   +  +   
Sbjct: 255 PSGYQNHYADGRPVTYDATYRNYDVYTAGLSEEEQLERALRASIWDR--GNTRNGPMPYG 312

Query: 309 LDLTWEEMRRRRLERF 314
             L  EEMRR+RL RF
Sbjct: 315 FRLPPEEMRRQRLHRF 312

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BLAST of CSPI04G20320 vs. ExPASy Swiss-Prot
Match: Q8TEB9 (Rhomboid-related protein 4 OS=Homo sapiens OX=9606 GN=RHBDD1 PE=1 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 6.2e-25
Identity = 81/234 (34.62%), Postives = 120/234 (51.28%), Query Frame = 0


Query: 20  PITLGLLASNTLIHLRPNF-LHHIIPSIDEVWFNAHLIFKHKDLKRFLLSPFYHVGDPHL 79
           P+TL  LA N    L P   L+    S+++        ++ KD +R LLSP +H  D HL
Sbjct: 29  PVTLATLALNIWFFLNPQKPLYSSCLSVEK-------CYQQKDWQRLLLSPLHHADDWHL 88

Query: 80  VYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLIKSLLIFFNYGRPYYYEYS 139
            +NM S+LWKG  LE  +GS  FA ++ A  +++ G+  LL++  +  F     +    +
Sbjct: 89  YFNMASMLWKGINLERRLGSRWFAYVITAFSVLT-GVVYLLLQFAVAEFMDEPDFKRSCA 148

Query: 140 VGFSGVLFAMKVVLGSE-TDSYTYVYGLRVPSSHAAWLELILIQMVSPGVSFLGHLGGIL 199
           VGFSGVLFA+KV+        +  + G  VP+  A W+EL+ I + SPG SF GHL GIL
Sbjct: 149 VGFSGVLFALKVLNNHYCPGGFVNILGFPVPNRFACWVELVAIHLFSPGTSFAGHLAGIL 208

Query: 200 AGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFRQRRIFGGGTVG 252
            GL++ +         PL  ++ +     S S G+     P RQ      G+ G
Sbjct: 209 VGLMYTQ--------GPLKKIMEACAGGFSSSVGY-----PGRQYYFNSSGSSG 241

[Close]

BLAST of CSPI04G20320 vs. ExPASy Swiss-Prot
Match: Q4V8F3 (Rhomboid-related protein 4 OS=Rattus norvegicus OX=10116 GN=Rhbdd1 PE=2 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 5.8e-23
Identity = 71/199 (35.68%), Postives = 107/199 (53.77%), Query Frame = 0


Query: 9   VVSQYYRLQGN---PITLGLLASNTLIHLRP-NFLHHIIPSIDEVWFNAHLIFKHKDLKR 68
           ++SQ + +  N   P+TL  LA N    L P   L+H   S+++        ++  D +R
Sbjct: 15  LLSQVFHIGINNIPPVTLATLAVNVWFFLNPWKPLYHSCISVEK-------CYQQNDWQR 74

Query: 69  FLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLIKSLL 128
            LLSP +H  D HL +NM+S+LWKG +LE  +GS  FA ++    +++ G+  LL++   
Sbjct: 75  LLLSPVHHGDDWHLYFNMVSMLWKGVKLEKRLGSRWFAYIIATFSLLT-GVVYLLLQFAS 134

Query: 129 IFFNYGRPYYYEYSVGFSGVLFAMKVVLGSE-TDSYTYVYGLRVPSSHAAWLELILIQMV 188
                   +    +VGFSGVLFA+KV+        +  + G  VP+  A W EL  I   
Sbjct: 135 AELMNQPDFKRNCAVGFSGVLFALKVLSNHYCPGGFVNILGFPVPNRFACWAELAAIHFC 194

Query: 189 SPGVSFLGHLGGILAGLLF 203
           +PG SF GHL GIL GL++
Sbjct: 195 TPGTSFAGHLAGILVGLMY 205

[Close]

BLAST of CSPI04G20320 vs. ExPASy Swiss-Prot
Match: Q5RBS4 (Rhomboid-related protein 4 OS=Pongo abelii OX=9601 GN=RHBDD1 PE=2 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 2.9e-22
Identity = 67/185 (36.22%), Postives = 101/185 (54.59%), Query Frame = 0


Query: 20  PITLGLLASNTLIHLRPNF-LHHIIPSIDEVWFNAHLIFKHKDLKRFLLSPFYHVGDPHL 79
           P+TL  LA N    L P   L+    S+++        ++ +D +R LLSP +H  D HL
Sbjct: 29  PVTLATLALNIWFFLNPQKPLYSSCLSVEK-------CYQQRDWQRLLLSPLHHADDWHL 88

Query: 80  VYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLIKSLLIFFNYGRPYYYEYS 139
            +N  S+LWKG  LE  +GS  FA ++    +++ G+  LL++  +  F     +    +
Sbjct: 89  YFNTASVLWKGINLERRLGSRWFAYVITTFSVLT-GVVYLLLQFAVAEFMDEPDFKRSCA 148

Query: 140 VGFSGVLFAMKVVLGSE-TDSYTYVYGLRVPSSHAAWLELILIQMVSPGVSFLGHLGGIL 199
           VGFSGVLFA+KV+        +  + G  VP+  A W+EL+ I + SPG SF GH  GIL
Sbjct: 149 VGFSGVLFALKVLNNHYCPGGFVNILGFPVPNRFACWVELVAIHLFSPGTSFAGHQAGIL 205

Query: 200 AGLLF 203
            GL++
Sbjct: 209 VGLMY 205

[Close]

BLAST of CSPI04G20320 vs. ExPASy TrEMBL
Match: A0A0A0L173 (RanBP2-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G506850 PE=3 SV=1)

HSP 1 Score: 630.2 bits (1624), Expect = 4.7e-177
Identity = 313/314 (99.68%), Postives = 314/314 (100.00%), Query Frame = 0


Query: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK 60
           MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK
Sbjct: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK 60

Query: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI 120
           DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI
Sbjct: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI 120

Query: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELILI 180
           KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYV+GLRVPSSHAAWLELILI
Sbjct: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVHGLRVPSSHAAWLELILI 180

Query: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR 240
           QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR
Sbjct: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR 240

Query: 241 QRRIFGGGTVGSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQLDL 300
           QRRIFGGGTVGSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQLDL
Sbjct: 241 QRRIFGGGTVGSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQLDL 300

Query: 301 TWEEMRRRRLERFG 315
           TWEEMRRRRLERFG
Sbjct: 301 TWEEMRRRRLERFG 314

[Close]

BLAST of CSPI04G20320 vs. ExPASy TrEMBL
Match: A0A5A7SVC3 (Rhomboid-like protein 14 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold83G001640 PE=3 SV=1)

HSP 1 Score: 605.5 bits (1560), Expect = 1.2e-169
Identity = 301/314 (95.86%), Postives = 307/314 (97.77%), Query Frame = 0


Query: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK 60
           MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLI KHK
Sbjct: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLILKHK 60

Query: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI 120
           DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALL+MSQGITLLLI
Sbjct: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLVMSQGITLLLI 120

Query: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELILI 180
           KSLLIFFNYGRPYYYEYSVGFSGVLFA+KVVLGSET+SYTY++GLRVPSSHAAWLELILI
Sbjct: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFALKVVLGSETESYTYIHGLRVPSSHAAWLELILI 180

Query: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR 240
           QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSR  GFF RLNPFR
Sbjct: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRPLGFFSRLNPFR 240

Query: 241 QRRIFGGGTVGSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQLDL 300
           QRRIFGGGTVGSRPVTSIGTWRC+ACTFDNSGLLS CEMCGTDRQDGVLHSNQLSSQLD 
Sbjct: 241 QRRIFGGGTVGSRPVTSIGTWRCRACTFDNSGLLSTCEMCGTDRQDGVLHSNQLSSQLDF 300

Query: 301 TWEEMRRRRLERFG 315
           T EEMRRRRLERFG
Sbjct: 301 TREEMRRRRLERFG 314

[Close]

BLAST of CSPI04G20320 vs. ExPASy TrEMBL
Match: A0A1S3B4I1 (rhomboid-like protein 14, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103486069 PE=3 SV=1)

HSP 1 Score: 605.5 bits (1560), Expect = 1.2e-169
Identity = 301/314 (95.86%), Postives = 307/314 (97.77%), Query Frame = 0


Query: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK 60
           MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLI KHK
Sbjct: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLILKHK 60

Query: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI 120
           DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALL+MSQGITLLLI
Sbjct: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLVMSQGITLLLI 120

Query: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELILI 180
           KSLLIFFNYGRPYYYEYSVGFSGVLFA+KVVLGSET+SYTY++GLRVPSSHAAWLELILI
Sbjct: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFALKVVLGSETESYTYIHGLRVPSSHAAWLELILI 180

Query: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR 240
           QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSR  GFF RLNPFR
Sbjct: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRPLGFFSRLNPFR 240

Query: 241 QRRIFGGGTVGSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQLDL 300
           QRRIFGGGTVGSRPVTSIGTWRC+ACTFDNSGLLS CEMCGTDRQDGVLHSNQLSSQLD 
Sbjct: 241 QRRIFGGGTVGSRPVTSIGTWRCRACTFDNSGLLSTCEMCGTDRQDGVLHSNQLSSQLDF 300

Query: 301 TWEEMRRRRLERFG 315
           T EEMRRRRLERFG
Sbjct: 301 TREEMRRRRLERFG 314

[Close]

BLAST of CSPI04G20320 vs. ExPASy TrEMBL
Match: A0A6J1HXX5 (rhomboid-like protein 14, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111467237 PE=3 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 7.1e-141
Identity = 256/314 (81.53%), Postives = 281/314 (89.49%), Query Frame = 0


Query: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK 60
           MLPLLAYQVV+QY RLQG PIT+GLLA+NTLIHLRP FL  +IPSID+VWFNA+LI K+K
Sbjct: 1   MLPLLAYQVVTQYNRLQGKPITVGLLATNTLIHLRPGFLRSVIPSIDDVWFNAYLILKYK 60

Query: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI 120
           DLKRF LSPFYHVGD HL YNMISLLWKG QLE+SMGS +FASMVGALL+MSQGITLLL+
Sbjct: 61  DLKRFFLSPFYHVGDSHLAYNMISLLWKGIQLESSMGSTKFASMVGALLVMSQGITLLLV 120

Query: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELILI 180
           KSLL FFNY RPYY+EYSVGFSGVLFAMKVVLGS+++SYTYV+G+RVPSSHAAWLELILI
Sbjct: 121 KSLL-FFNYERPYYHEYSVGFSGVLFAMKVVLGSQSESYTYVHGVRVPSSHAAWLELILI 180

Query: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR 240
           Q+VSPGVSFLGHLGGILAG+LFLRLKG NSGSDPL +LIRSVGRILSR  GFFCRLNPFR
Sbjct: 181 QIVSPGVSFLGHLGGILAGILFLRLKGMNSGSDPLAVLIRSVGRILSRPLGFFCRLNPFR 240

Query: 241 QRRIFGGGTVGSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQLDL 300
             RIFGGGTVG R  TS+ TWRCQACTFDNS LL  CEMCGTDRQD  + S +LS + DL
Sbjct: 241 HHRIFGGGTVGRRQ-TSVDTWRCQACTFDNSCLLPRCEMCGTDRQDRAMPSRRLSHRFDL 300

Query: 301 TWEEMRRRRLERFG 315
           T EE+RRRRLERFG
Sbjct: 301 TPEELRRRRLERFG 312

[Close]

BLAST of CSPI04G20320 vs. ExPASy TrEMBL
Match: A0A6J1EL30 (rhomboid-like protein 14, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111435540 PE=3 SV=1)

HSP 1 Score: 506.9 bits (1304), Expect = 6.0e-140
Identity = 254/314 (80.89%), Postives = 281/314 (89.49%), Query Frame = 0


Query: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK 60
           MLPLLAYQVV+QY RLQG PIT+GLLA+NTLIHLRP FL  +IPSID+VWFNA+LI K+K
Sbjct: 1   MLPLLAYQVVTQYNRLQGKPITVGLLATNTLIHLRPGFLRSVIPSIDDVWFNAYLILKYK 60

Query: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI 120
           DLKRF LSPFYHVGD HL YNMISLLWKG QLE+SMGS +FASMVGALL+MSQGITLLL+
Sbjct: 61  DLKRFFLSPFYHVGDSHLAYNMISLLWKGIQLESSMGSTKFASMVGALLVMSQGITLLLV 120

Query: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELILI 180
           KSLL FFNY +PYY+EYSVGFSGVLFAMKVVLGS+++SYTYV+G+RVPSSHAAWLELILI
Sbjct: 121 KSLL-FFNYEKPYYHEYSVGFSGVLFAMKVVLGSQSESYTYVHGVRVPSSHAAWLELILI 180

Query: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR 240
           Q+VSPGVSFLGHLGGILAG+LFLRLKG N GSDPL +LIRSVGRILSR  GFFCRLNPFR
Sbjct: 181 QIVSPGVSFLGHLGGILAGILFLRLKGMNLGSDPLAVLIRSVGRILSRPLGFFCRLNPFR 240

Query: 241 QRRIFGGGTVGSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQLDL 300
            RRIFGGGTVG R  TS+ TWRC+ACTFDNS LL  CEMCGTDRQD  + S +LS + DL
Sbjct: 241 HRRIFGGGTVGRRQ-TSVDTWRCRACTFDNSCLLPRCEMCGTDRQDRAMPSRRLSHRFDL 300

Query: 301 TWEEMRRRRLERFG 315
           T EE+RRRRLERFG
Sbjct: 301 TPEELRRRRLERFG 312

[Close]

BLAST of CSPI04G20320 vs. NCBI nr
Match: XP_004146389.1 (rhomboid-like protein 14, mitochondrial [Cucumis sativus] >XP_011653901.1 rhomboid-like protein 14, mitochondrial [Cucumis sativus] >KGN54804.1 hypothetical protein Csa_012505 [Cucumis sativus])

HSP 1 Score: 630.2 bits (1624), Expect = 9.8e-177
Identity = 313/314 (99.68%), Postives = 314/314 (100.00%), Query Frame = 0


Query: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK 60
           MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK
Sbjct: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK 60

Query: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI 120
           DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI
Sbjct: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI 120

Query: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELILI 180
           KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYV+GLRVPSSHAAWLELILI
Sbjct: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVHGLRVPSSHAAWLELILI 180

Query: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR 240
           QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR
Sbjct: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR 240

Query: 241 QRRIFGGGTVGSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQLDL 300
           QRRIFGGGTVGSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQLDL
Sbjct: 241 QRRIFGGGTVGSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQLDL 300

Query: 301 TWEEMRRRRLERFG 315
           TWEEMRRRRLERFG
Sbjct: 301 TWEEMRRRRLERFG 314

[Close]

BLAST of CSPI04G20320 vs. NCBI nr
Match: XP_008442110.1 (PREDICTED: rhomboid-like protein 14, mitochondrial [Cucumis melo] >KAA0034920.1 rhomboid-like protein 14 [Cucumis melo var. makuwa] >TYK05460.1 rhomboid-like protein 14 [Cucumis melo var. makuwa])

HSP 1 Score: 605.5 bits (1560), Expect = 2.6e-169
Identity = 301/314 (95.86%), Postives = 307/314 (97.77%), Query Frame = 0


Query: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK 60
           MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLI KHK
Sbjct: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLILKHK 60

Query: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI 120
           DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALL+MSQGITLLLI
Sbjct: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLVMSQGITLLLI 120

Query: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELILI 180
           KSLLIFFNYGRPYYYEYSVGFSGVLFA+KVVLGSET+SYTY++GLRVPSSHAAWLELILI
Sbjct: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFALKVVLGSETESYTYIHGLRVPSSHAAWLELILI 180

Query: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR 240
           QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSR  GFF RLNPFR
Sbjct: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRPLGFFSRLNPFR 240

Query: 241 QRRIFGGGTVGSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQLDL 300
           QRRIFGGGTVGSRPVTSIGTWRC+ACTFDNSGLLS CEMCGTDRQDGVLHSNQLSSQLD 
Sbjct: 241 QRRIFGGGTVGSRPVTSIGTWRCRACTFDNSGLLSTCEMCGTDRQDGVLHSNQLSSQLDF 300

Query: 301 TWEEMRRRRLERFG 315
           T EEMRRRRLERFG
Sbjct: 301 TREEMRRRRLERFG 314

[Close]

BLAST of CSPI04G20320 vs. NCBI nr
Match: XP_038881196.1 (rhomboid-like protein 14, mitochondrial [Benincasa hispida])

HSP 1 Score: 553.9 bits (1426), Expect = 8.9e-154
Identity = 278/314 (88.54%), Postives = 291/314 (92.68%), Query Frame = 0


Query: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK 60
           MLPLLAYQVVSQYYRLQ NPIT+GLLA+NTLIHLRPNFL HIIPSID+VWFNAHLI KHK
Sbjct: 1   MLPLLAYQVVSQYYRLQRNPITVGLLATNTLIHLRPNFLRHIIPSIDDVWFNAHLILKHK 60

Query: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI 120
           DLKRFLLSPFYHVGDPHLVYNMISLLWKG QLETSMGSIEFASMV ALL+MSQGITLLLI
Sbjct: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGIQLETSMGSIEFASMVSALLVMSQGITLLLI 120

Query: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELILI 180
           KSLL+ FNY RPYYYEYSVGFSGVLFAMKVVLGS+ +SYTY++G+RVPSSHAAWLELILI
Sbjct: 121 KSLLV-FNYDRPYYYEYSVGFSGVLFAMKVVLGSQAESYTYLHGIRVPSSHAAWLELILI 180

Query: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR 240
           QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSD LTLLIR  G+ILSR  GFFCRLNPFR
Sbjct: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDLLTLLIRRAGQILSRPLGFFCRLNPFR 240

Query: 241 QRRIFGGGTVGSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQLDL 300
           QRRIFGGGTVGSRP TSI TWRCQACTFDNSGLLS CEMCGTDRQD VL  + LS Q DL
Sbjct: 241 QRRIFGGGTVGSRPATSIDTWRCQACTFDNSGLLSICEMCGTDRQDRVLPLHHLSRQFDL 300

Query: 301 TWEEMRRRRLERFG 315
           T EE+RRRRLERFG
Sbjct: 301 TREEVRRRRLERFG 313

[Close]

BLAST of CSPI04G20320 vs. NCBI nr
Match: XP_022967849.1 (rhomboid-like protein 14, mitochondrial [Cucurbita maxima] >XP_022967850.1 rhomboid-like protein 14, mitochondrial [Cucurbita maxima] >XP_022967852.1 rhomboid-like protein 14, mitochondrial [Cucurbita maxima] >XP_022967853.1 rhomboid-like protein 14, mitochondrial [Cucurbita maxima] >XP_022967854.1 rhomboid-like protein 14, mitochondrial [Cucurbita maxima])

HSP 1 Score: 510.0 bits (1312), Expect = 1.5e-140
Identity = 256/314 (81.53%), Postives = 281/314 (89.49%), Query Frame = 0


Query: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK 60
           MLPLLAYQVV+QY RLQG PIT+GLLA+NTLIHLRP FL  +IPSID+VWFNA+LI K+K
Sbjct: 1   MLPLLAYQVVTQYNRLQGKPITVGLLATNTLIHLRPGFLRSVIPSIDDVWFNAYLILKYK 60

Query: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI 120
           DLKRF LSPFYHVGD HL YNMISLLWKG QLE+SMGS +FASMVGALL+MSQGITLLL+
Sbjct: 61  DLKRFFLSPFYHVGDSHLAYNMISLLWKGIQLESSMGSTKFASMVGALLVMSQGITLLLV 120

Query: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELILI 180
           KSLL FFNY RPYY+EYSVGFSGVLFAMKVVLGS+++SYTYV+G+RVPSSHAAWLELILI
Sbjct: 121 KSLL-FFNYERPYYHEYSVGFSGVLFAMKVVLGSQSESYTYVHGVRVPSSHAAWLELILI 180

Query: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR 240
           Q+VSPGVSFLGHLGGILAG+LFLRLKG NSGSDPL +LIRSVGRILSR  GFFCRLNPFR
Sbjct: 181 QIVSPGVSFLGHLGGILAGILFLRLKGMNSGSDPLAVLIRSVGRILSRPLGFFCRLNPFR 240

Query: 241 QRRIFGGGTVGSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQLDL 300
             RIFGGGTVG R  TS+ TWRCQACTFDNS LL  CEMCGTDRQD  + S +LS + DL
Sbjct: 241 HHRIFGGGTVGRRQ-TSVDTWRCQACTFDNSCLLPRCEMCGTDRQDRAMPSRRLSHRFDL 300

Query: 301 TWEEMRRRRLERFG 315
           T EE+RRRRLERFG
Sbjct: 301 TPEELRRRRLERFG 312

[Close]

BLAST of CSPI04G20320 vs. NCBI nr
Match: KAG6603179.1 (Rhomboid-like protein 14, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033491.1 Rhomboid-like protein 14, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 507.7 bits (1306), Expect = 7.3e-140
Identity = 254/314 (80.89%), Postives = 281/314 (89.49%), Query Frame = 0


Query: 1   MLPLLAYQVVSQYYRLQGNPITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKHK 60
           MLPLLAYQVV+QY RLQG PIT+GLLA+NTLIHLRP FL  +IPSID+VWFNA+LI K+K
Sbjct: 1   MLPLLAYQVVTQYNRLQGKPITVGLLATNTLIHLRPGFLRSVIPSIDDVWFNAYLILKYK 60

Query: 61  DLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLI 120
           DLKRF LSPFYHVGD HL YNMISLLWKG QLE+SMGS +FASMVG LL+MSQGITLLL+
Sbjct: 61  DLKRFFLSPFYHVGDSHLAYNMISLLWKGIQLESSMGSTKFASMVGVLLVMSQGITLLLV 120

Query: 121 KSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELILI 180
           KSLL FFNY +PYY+EYSVGFSGVLFAMKVVLGS+++SYTYV+G+RVPSSHAAWLELILI
Sbjct: 121 KSLL-FFNYEKPYYHEYSVGFSGVLFAMKVVLGSQSESYTYVHGVRVPSSHAAWLELILI 180

Query: 181 QMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFR 240
           Q+VSPGVSFLGHLGGILAG+LFLRLKG NSGSDPL +LIRSVGRILSR  GFFCRLNPFR
Sbjct: 181 QIVSPGVSFLGHLGGILAGILFLRLKGMNSGSDPLAVLIRSVGRILSRPLGFFCRLNPFR 240

Query: 241 QRRIFGGGTVGSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDGVLHSNQLSSQLDL 300
            RRIFGGGTVG R  TS+ TWRC+ACTFDNS LL  CEMCGTDRQD  + S +LS + DL
Sbjct: 241 HRRIFGGGTVGRRQ-TSVDTWRCRACTFDNSCLLPRCEMCGTDRQDRAMPSRRLSHRFDL 300

Query: 301 TWEEMRRRRLERFG 315
           T EE+RRRRLERFG
Sbjct: 301 TPEELRRRRLERFG 312

[Close]

BLAST of CSPI04G20320 vs. TAIR 10
Match: AT3G17611.1 (RHOMBOID-like protein 14 )

HSP 1 Score: 373.6 bits (958), Expect = 1.5e-103
Identity = 193/320 (60.31%), Postives = 237/320 (74.06%), Query Frame = 0


Query: 1   MLPLLAYQVVSQYYRLQGN-PITLGLLASNTLIHLRPNFLHHIIPSIDEVWFNAHLIFKH 60
           MLPLLA   V++YYRL    P+T  LLA+NTL++LRP F+  +IP I EVWFN HLIFKH
Sbjct: 14  MLPLLALSAVAEYYRLPWKPPVTASLLAANTLVYLRPAFIDPVIPHISEVWFNPHLIFKH 73

Query: 61  KDLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLL 120
           KDLKR  LS FYHV +PHLVYNM+SLLWKG +LETSMGS EFASMV  L+ MSQG+TLLL
Sbjct: 74  KDLKRLFLSAFYHVNEPHLVYNMMSLLWKGIKLETSMGSSEFASMVFTLIGMSQGVTLLL 133

Query: 121 IKSLLIFFNYGRPYYYEYSVGFSGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELIL 180
            KSLL+ F+Y R YY EY+VGFSGVLFAMKVVL S+ + Y+ VYG+ VP+ +AAW ELIL
Sbjct: 134 AKSLLLLFDYDRAYYNEYAVGFSGVLFAMKVVLNSQAEDYSSVYGILVPTKYAAWAELIL 193

Query: 181 IQMVSPGVSFLGHLGGILAGLLFLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPF 240
           +QM  P  SFLGHLGGILAG+++L+LKG+ SGSDP+T+ +R V R+++    F   +   
Sbjct: 194 VQMFVPNASFLGHLGGILAGIIYLKLKGSYSGSDPVTMAVRGVSRLVTWPLRFLNGMVRS 253

Query: 241 RQRRIFGGGTV--GSRPVTSIGTWRCQACTFDNSGLLSACEMCGTDRQDG----VLHSNQ 300
           R+RRI G G V  G   +   G WRCQ+CT+DNSG LSACEMCG+ R  G    +     
Sbjct: 254 RRRRITGRGRVGRGQTGIAGPGIWRCQSCTYDNSGWLSACEMCGSGRARGNGWSLNQGPA 313

Query: 301 LSSQLDLTWEEMRRRRLERF 314
           LSS  DL  +E+RRRR+ERF
Sbjct: 314 LSSSNDLPLDELRRRRVERF 333

[Close]

BLAST of CSPI04G20320 vs. TAIR 10
Match: AT3G17611.2 (RHOMBOID-like protein 14 )

HSP 1 Score: 263.8 bits (673), Expect = 1.7e-70
Identity = 139/238 (58.40%), Postives = 174/238 (73.11%), Query Frame = 0


Query: 82  MISLLWKGTQLETSMGSIEFASMVGALLIMSQGITLLLIKSLLIFFNYGRPYYYEYSVGF 141
           M+SLLWKG +LETSMGS EFASMV  L+ MSQG+TLLL KSLL+ F+Y R YY EY+VGF
Sbjct: 1   MMSLLWKGIKLETSMGSSEFASMVFTLIGMSQGVTLLLAKSLLLLFDYDRAYYNEYAVGF 60

Query: 142 SGVLFAMKVVLGSETDSYTYVYGLRVPSSHAAWLELILIQMVSPGVSFLGHLGGILAGLL 201
           SGVLFAMKVVL S+ + Y+ VYG+ VP+ +AAW ELIL+QM  P  SFLGHLGGILAG++
Sbjct: 61  SGVLFAMKVVLNSQAEDYSSVYGILVPTKYAAWAELILVQMFVPNASFLGHLGGILAGII 120

Query: 202 FLRLKGTNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFRQRRIFGGGTV--GSRPVTSIG 261
           +L+LKG+ SGSDP+T+ +R V R+++    F   +   R+RRI G G V  G   +   G
Sbjct: 121 YLKLKGSYSGSDPVTMAVRGVSRLVTWPLRFLNGMVRSRRRRITGRGRVGRGQTGIAGPG 180

Query: 262 TWRCQACTFDNSGLLSACEMCGTDRQDG----VLHSNQLSSQLDLTWEEMRRRRLERF 314
            WRCQ+CT+DNSG LSACEMCG+ R  G    +     LSS  DL  +E+RRRR+ERF
Sbjct: 181 IWRCQSCTYDNSGWLSACEMCGSGRARGNGWSLNQGPALSSSNDLPLDELRRRRVERF 238

[Close]

BLAST of CSPI04G20320 vs. TAIR 10
Match: AT3G17611.3 (RHOMBOID-like protein 14 )

HSP 1 Score: 174.9 bits (442), Expect = 1.0e-43
Identity = 90/172 (52.33%), Postives = 118/172 (68.60%), Query Frame = 0


Query: 148 MKVVLGSETDSYTYVYGLRVPSSHAAWLELILIQMVSPGVSFLGHLGGILAGLLFLRLKG 207
           MKVVL S+ + Y+ VYG+ VP+ +AAW ELIL+QM  P  SFLGHLGGILAG+++L+LKG
Sbjct: 1   MKVVLNSQAEDYSSVYGILVPTKYAAWAELILVQMFVPNASFLGHLGGILAGIIYLKLKG 60

Query: 208 TNSGSDPLTLLIRSVGRILSRSYGFFCRLNPFRQRRIFGGGTV--GSRPVTSIGTWRCQA 267
           + SGSDP+T+ +R V R+++    F   +   R+RRI G G V  G   +   G WRCQ+
Sbjct: 61  SYSGSDPVTMAVRGVSRLVTWPLRFLNGMVRSRRRRITGRGRVGRGQTGIAGPGIWRCQS 120

Query: 268 CTFDNSGLLSACEMCGTDRQDG----VLHSNQLSSQLDLTWEEMRRRRLERF 314
           CT+DNSG LSACEMCG+ R  G    +     LSS  DL  +E+RRRR+ERF
Sbjct: 121 CTYDNSGWLSACEMCGSGRARGNGWSLNQGPALSSSNDLPLDELRRRRVERF 172

[Close]

BLAST of CSPI04G20320 vs. TAIR 10
Match: AT3G58460.1 (RHOMBOID-like protein 15 )

HSP 1 Score: 73.2 bits (178), Expect = 4.3e-13
Identity = 55/162 (33.95%), Postives = 87/162 (53.70%), Query Frame = 0


Query: 48  EVWFNAHLIFKHKDLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGA 107
           EV F    I     + RF  +  +H    H+++NM++L+  G++LE  MGS+    +   
Sbjct: 50  EVCFLPSAIISRFQVYRFYTAIIFHGSLLHVLFNMMALVPMGSELERIMGSVRLLYLT-V 109

Query: 108 LLIMSQGITLLLIKSLLIFFNYGRPYYYEY-----SVGFSGVLFAMKVVLGSETD-SYTY 167
           LL  +  +  LLI SL     Y   Y Y++     ++GFSG+LF+M V+  S +  +   
Sbjct: 110 LLATTNAVLHLLIASLA---GYNPFYQYDHLMNECAIGFSGILFSMIVIETSLSGVTSRS 169

Query: 168 VYGL-RVPSSHAAWLELILIQMVSPGVSFLGHLGGILAGLLF 203
           V+GL  VP+    W+ LI+ Q++   VS LGHL GIL+G  +
Sbjct: 170 VFGLFNVPAKLYPWILLIVFQLLMTNVSLLGHLCGILSGFSY 207

[Close]

BLAST of CSPI04G20320 vs. TAIR 10
Match: AT3G58460.2 (RHOMBOID-like protein 15 )

HSP 1 Score: 73.2 bits (178), Expect = 4.3e-13
Identity = 55/162 (33.95%), Postives = 87/162 (53.70%), Query Frame = 0


Query: 48  EVWFNAHLIFKHKDLKRFLLSPFYHVGDPHLVYNMISLLWKGTQLETSMGSIEFASMVGA 107
           EV F    I     + RF  +  +H    H+++NM++L+  G++LE  MGS+    +   
Sbjct: 73  EVCFLPSAIISRFQVYRFYTAIIFHGSLLHVLFNMMALVPMGSELERIMGSVRLLYLT-V 132

Query: 108 LLIMSQGITLLLIKSLLIFFNYGRPYYYEY-----SVGFSGVLFAMKVVLGSETD-SYTY 167
           LL  +  +  LLI SL     Y   Y Y++     ++GFSG+LF+M V+  S +  +   
Sbjct: 133 LLATTNAVLHLLIASLA---GYNPFYQYDHLMNECAIGFSGILFSMIVIETSLSGVTSRS 192

Query: 168 VYGL-RVPSSHAAWLELILIQMVSPGVSFLGHLGGILAGLLF 203
           V+GL  VP+    W+ LI+ Q++   VS LGHL GIL+G  +
Sbjct: 193 VFGLFNVPAKLYPWILLIVFQLLMTNVSLLGHLCGILSGFSY 230

The following BLAST results are available for this feature:

BLAST of CSPI04G20320 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RXW0	2.2e-102	60.31	Rhomboid-like protein 14, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=RBL14...	[more]
Q8BHC7	2.5e-26	31.96	Rhomboid-related protein 4 OS=Mus musculus OX=10090 GN=Rhbdd1 PE=1 SV=1	[more]
Q8TEB9	6.2e-25	34.62	Rhomboid-related protein 4 OS=Homo sapiens OX=9606 GN=RHBDD1 PE=1 SV=1	[more]
Q4V8F3	5.8e-23	35.68	Rhomboid-related protein 4 OS=Rattus norvegicus OX=10116 GN=Rhbdd1 PE=2 SV=1	[more]
Q5RBS4	2.9e-22	36.22	Rhomboid-related protein 4 OS=Pongo abelii OX=9601 GN=RHBDD1 PE=2 SV=1	[more]

BLAST of CSPI04G20320 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L173	4.7e-177	99.68	RanBP2-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G506850...	[more]
A0A5A7SVC3	1.2e-169	95.86	Rhomboid-like protein 14 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A1S3B4I1	1.2e-169	95.86	rhomboid-like protein 14, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103486069 ...	[more]
A0A6J1HXX5	7.1e-141	81.53	rhomboid-like protein 14, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111467...	[more]
A0A6J1EL30	6.0e-140	80.89	rhomboid-like protein 14, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]

BLAST of CSPI04G20320 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004146389.1	9.8e-177	99.68	rhomboid-like protein 14, mitochondrial [Cucumis sativus] >XP_011653901.1 rhombo...	[more]
XP_008442110.1	2.6e-169	95.86	PREDICTED: rhomboid-like protein 14, mitochondrial [Cucumis melo] >KAA0034920.1 ...	[more]
XP_038881196.1	8.9e-154	88.54	rhomboid-like protein 14, mitochondrial [Benincasa hispida]	[more]
XP_022967849.1	1.5e-140	81.53	rhomboid-like protein 14, mitochondrial [Cucurbita maxima] >XP_022967850.1 rhomb...	[more]
KAG6603179.1	7.3e-140	80.89	Rhomboid-like protein 14, mitochondrial, partial [Cucurbita argyrosperma subsp. ...	[more]

BLAST of CSPI04G20320 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G17611.1	1.5e-103	60.31	RHOMBOID-like protein 14	[more]
AT3G17611.2	1.7e-70	58.40	RHOMBOID-like protein 14	[more]
AT3G17611.3	1.0e-43	52.33	RHOMBOID-like protein 14	[more]
AT3G58460.1	4.3e-13	33.95	RHOMBOID-like protein 15	[more]
AT3G58460.2	4.3e-13	33.95	RHOMBOID-like protein 15	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001876	Zinc finger, RanBP2-type	SMART	SM00547	zf_4	coord: 259..283 e-value: 4.5E-4 score: 29.5
IPR001876	Zinc finger, RanBP2-type	PFAM	PF00641	zf-RanBP	coord: 258..286 e-value: 5.5E-7 score: 28.8
IPR001876	Zinc finger, RanBP2-type	PROSITE	PS01358	ZF_RANBP2_1	coord: 261..280
IPR001876	Zinc finger, RanBP2-type	PROSITE	PS50199	ZF_RANBP2_2	coord: 257..286 score: 8.738674
IPR022764	Peptidase S54, rhomboid domain	PFAM	PF01694	Rhomboid	coord: 58..204 e-value: 2.1E-20 score: 73.3
IPR035952	Rhomboid-like superfamily	GENE3D	1.20.1540.10		coord: 12..209 e-value: 2.2E-22 score: 81.6
IPR035952	Rhomboid-like superfamily	SUPERFAMILY	144091	Rhomboid-like	coord: 19..204
None	No IPR available	GENE3D	2.30.30.380		coord: 254..312 e-value: 2.4E-8 score: 35.6
None	No IPR available	PANTHER	PTHR43066:SF1	RHOMBOID PROTEIN 2	coord: 1..312
None	No IPR available	PANTHER	PTHR43066	RHOMBOID-RELATED PROTEIN	coord: 1..312
IPR036443	Zinc finger, RanBP2-type superfamily	SUPERFAMILY	90209	Ran binding protein zinc finger-like	coord: 260..284

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI04G20320.2	CSPI04G20320.2	mRNA
CSPI04G20320.1	CSPI04G20320.1	mRNA
CSPI04G20320.3	CSPI04G20320.3	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004252	serine-type endopeptidase activity