CSPI04G01210.1 (mRNA) Cucumber (PI 183967) v1

Overview


Name	CSPI04G01210.1
Type	mRNA
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	E3 ubiquitin-protein ligase RNF181-like protein
Location	Chr4: 682182 .. 682790 (-)
Sequence length	609
RNA-Seq Expression	CSPI04G01210.1
Synteny	CSPI04G01210.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTTTTGAAATTTCTTCTCCAATTCCATATCTTCTCAACGCTAATGGTTTCGGCAATGGAGTATTGGAAGTTCAGTTAAGTTATCTGGAACGTGCAATTTCCGTTTCTTCATTCTCCCAACGAATCCTCTACGAAACTCAACCCACTCCATTCAACAATGCACTCTTCTATCTCTCTGTGCACCAACTTCAACATCCCTTGTTCCACATTCGGCAATTTCTCTACTCACTCAACATTCGAAGGGAAACCATAGCTCGGCAAATCGCTTCCTACGTCGCCCACATGTGTAACGGGAACACGGGGTGGAATTCGAATTTCTATGTCATCGCTAGAGTGGATTTGGTCCGAGTGATCCGGATAGAAGAACAACCAAGGGCGGAAGGATGGAGAGGAGTGGCGGTGGAGAGGTTGAGTAAGTTGAAATCGGAGGAGGAAAAGGGAGATTGCAGTGTGTGTTTGGATGAATTGGATTGTGAAAAGAGGGAGGTTATAAGGATTCCTTGCGGCCATGTTTACCATGAATCGTGCATCTTCAAGTGGCTTAACAGCAGTAACTCTTGCCCTTTGTGTAGAAGCTCATTCCCACTACACAATGTCACAAACTGA

mRNA sequence

ATGTCTTTTGAAATTTCTTCTCCAATTCCATATCTTCTCAACGCTAATGGTTTCGGCAATGGAGTATTGGAAGTTCAGTTAAGTTATCTGGAACGTGCAATTTCCGTTTCTTCATTCTCCCAACGAATCCTCTACGAAACTCAACCCACTCCATTCAACAATGCACTCTTCTATCTCTCTGTGCACCAACTTCAACATCCCTTGTTCCACATTCGGCAATTTCTCTACTCACTCAACATTCGAAGGGAAACCATAGCTCGGCAAATCGCTTCCTACGTCGCCCACATGTGTAACGGGAACACGGGGTGGAATTCGAATTTCTATGTCATCGCTAGAGTGGATTTGGTCCGAGTGATCCGGATAGAAGAACAACCAAGGGCGGAAGGATGGAGAGGAGTGGCGGTGGAGAGGTTGAGTAAGTTGAAATCGGAGGAGGAAAAGGGAGATTGCAGTGTGTGTTTGGATGAATTGGATTGTGAAAAGAGGGAGGTTATAAGGATTCCTTGCGGCCATGTTTACCATGAATCGTGCATCTTCAAGTGGCTTAACAGCAGTAACTCTTGCCCTTTGTGTAGAAGCTCATTCCCACTACACAATGTCACAAACTGA

Coding sequence (CDS)

ATGTCTTTTGAAATTTCTTCTCCAATTCCATATCTTCTCAACGCTAATGGTTTCGGCAATGGAGTATTGGAAGTTCAGTTAAGTTATCTGGAACGTGCAATTTCCGTTTCTTCATTCTCCCAACGAATCCTCTACGAAACTCAACCCACTCCATTCAACAATGCACTCTTCTATCTCTCTGTGCACCAACTTCAACATCCCTTGTTCCACATTCGGCAATTTCTCTACTCACTCAACATTCGAAGGGAAACCATAGCTCGGCAAATCGCTTCCTACGTCGCCCACATGTGTAACGGGAACACGGGGTGGAATTCGAATTTCTATGTCATCGCTAGAGTGGATTTGGTCCGAGTGATCCGGATAGAAGAACAACCAAGGGCGGAAGGATGGAGAGGAGTGGCGGTGGAGAGGTTGAGTAAGTTGAAATCGGAGGAGGAAAAGGGAGATTGCAGTGTGTGTTTGGATGAATTGGATTGTGAAAAGAGGGAGGTTATAAGGATTCCTTGCGGCCATGTTTACCATGAATCGTGCATCTTCAAGTGGCTTAACAGCAGTAACTCTTGCCCTTTGTGTAGAAGCTCATTCCCACTACACAATGTCACAAACTGA

Protein sequence

MSFEISSPIPYLLNANGFGNGVLEVQLSYLERAISVSSFSQRILYETQPTPFNNALFYLSVHQLQHPLFHIRQFLYSLNIRRETIARQIASYVAHMCNGNTGWNSNFYVIARVDLVRVIRIEEQPRAEGWRGVAVERLSKLKSEEEKGDCSVCLDELDCEKREVIRIPCGHVYHESCIFKWLNSSNSCPLCRSSFPLHNVTN*

Homology

[Close]

BLAST of CSPI04G01210.1 vs. ExPASy Swiss-Prot
Match: Q9CY62 (E3 ubiquitin-protein ligase RNF181 OS=Mus musculus OX=10090 GN=Rnf181 PE=1 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 9.0e-09
Identity = 29/64 (45.31%), Postives = 38/64 (59.38%), Query Frame = 0


Query: 135 VERLSKLKSEEEKGD--CSVCLDELDCEKREVIRIPCGHVYHESCIFKWLNSSNSCPLCR 194
           VE L +      K D  C VCL E + E+  VI +PC H++H +CI  WL+ +NSCPLCR
Sbjct: 71  VESLPRTVISSAKADLKCPVCLLEFEAEE-TVIEMPCHHLFHSNCILPWLSKTNSCPLCR 130

Query: 195 SSFP 197
              P
Sbjct: 131 HELP 133

[Close]

BLAST of CSPI04G01210.1 vs. ExPASy Swiss-Prot
Match: Q3T0W3 (E3 ubiquitin-protein ligase RNF181 OS=Bos taurus OX=9913 GN=RNF181 PE=2 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.2e-08
Identity = 28/70 (40.00%), Postives = 42/70 (60.00%), Query Frame = 0


Query: 134 AVERLSK--LKSEEEKGDCSVCLDELDCEKREVIRIPCGHVYHESCIFKWLNSSNSCPLC 193
           AVE L +  ++  + +  C VCL E + E+   I +PC H++H +CI  WL+ +NSCPLC
Sbjct: 58  AVENLPRTVIRGSQAELKCPVCLLEFE-EEETAIEMPCHHLFHSNCILPWLSKTNSCPLC 117

Query: 194 RSSFPLHNVT 202
           R   P  + T
Sbjct: 118 RHELPTDDDT 126

[Close]

BLAST of CSPI04G01210.1 vs. ExPASy Swiss-Prot
Match: Q6AXU4 (E3 ubiquitin-protein ligase RNF181 OS=Rattus norvegicus OX=10116 GN=Rnf181 PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.2e-08
Identity = 25/56 (44.64%), Postives = 37/56 (66.07%), Query Frame = 0


Query: 141 LKSEEEKGDCSVCLDELDCEKREVIRIPCGHVYHESCIFKWLNSSNSCPLCRSSFP 197
           ++S + +  C VCL E + E+  VI +PC H++H +CI  WL+ +NSCPLCR   P
Sbjct: 79  IRSSKAELKCPVCLLEFE-EEETVIEMPCHHLFHSNCILPWLSKTNSCPLCRHELP 133

[Close]

BLAST of CSPI04G01210.1 vs. ExPASy Swiss-Prot
Match: Q5M974 (E3 ubiquitin-protein ligase RNF181 OS=Xenopus tropicalis OX=8364 GN=rnf181 PE=2 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.2e-08
Identity = 31/67 (46.27%), Postives = 40/67 (59.70%), Query Frame = 0


Query: 135 VERLSKLKSEEEKGD----CSVCLDELDCEKREVIR-IPCGHVYHESCIFKWLNSSNSCP 194
           VE L K+    E+ D    C VCL  L+ E+ E +R +PC H++H SCI  WL  +NSCP
Sbjct: 60  VESLPKVTVTPEQADAALKCPVCL--LEFEEGETVRQLPCEHLFHSSCILPWLGKTNSCP 119

Query: 195 LCRSSFP 197
           LCR   P
Sbjct: 120 LCRHELP 124

[Close]

BLAST of CSPI04G01210.1 vs. ExPASy Swiss-Prot
Match: Q9P0P0 (E3 ubiquitin-protein ligase RNF181 OS=Homo sapiens OX=9606 GN=RNF181 PE=1 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 2.0e-08
Identity = 28/69 (40.58%), Postives = 41/69 (59.42%), Query Frame = 0


Query: 135 VERLSK--LKSEEEKGDCSVCLDELDCEKREVIRIPCGHVYHESCIFKWLNSSNSCPLCR 194
           VE L +  ++  + +  C VCL E + E+   I +PC H++H SCI  WL+ +NSCPLCR
Sbjct: 59  VENLPRTVIRGSQAELKCPVCLLEFE-EEETAIEMPCHHLFHSSCILPWLSKTNSCPLCR 118

Query: 195 SSFPLHNVT 202
              P  + T
Sbjct: 119 YELPTDDDT 126

[Close]

BLAST of CSPI04G01210.1 vs. ExPASy TrEMBL
Match: A0A0A0KYC0 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G003650 PE=4 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 6.9e-113
Identity = 201/202 (99.50%), Postives = 202/202 (100.00%), Query Frame = 0


Query: 1   MSFEISSPIPYLLNANGFGNGVLEVQLSYLERAISVSSFSQRILYETQPTPFNNALFYLS 60
           MSFEISSPIPYLLNANGFGNGVLEVQLSYLERAISVSSFSQRILYETQPTPFNNALFYLS
Sbjct: 1   MSFEISSPIPYLLNANGFGNGVLEVQLSYLERAISVSSFSQRILYETQPTPFNNALFYLS 60

Query: 61  VHQLQHPLFHIRQFLYSLNIRRETIARQIASYVAHMCNGNTGWNSNFYVIARVDLVRVIR 120
           VHQLQHPLFHIRQFLYSLNIRRETIARQIAS+VAHMCNGNTGWNSNFYVIARVDLVRVIR
Sbjct: 61  VHQLQHPLFHIRQFLYSLNIRRETIARQIASFVAHMCNGNTGWNSNFYVIARVDLVRVIR 120

Query: 121 IEEQPRAEGWRGVAVERLSKLKSEEEKGDCSVCLDELDCEKREVIRIPCGHVYHESCIFK 180
           IEEQPRAEGWRGVAVERLSKLKSEEEKGDCSVCLDELDCEKREVIRIPCGHVYHESCIFK
Sbjct: 121 IEEQPRAEGWRGVAVERLSKLKSEEEKGDCSVCLDELDCEKREVIRIPCGHVYHESCIFK 180

Query: 181 WLNSSNSCPLCRSSFPLHNVTN 203
           WLNSSNSCPLCRSSFPLHNVTN
Sbjct: 181 WLNSSNSCPLCRSSFPLHNVTN 202

[Close]

BLAST of CSPI04G01210.1 vs. ExPASy TrEMBL
Match: A0A5A7TLM1 (E3 ubiquitin-protein ligase RNF181-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001430 PE=4 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 1.2e-85
Identity = 168/221 (76.02%), Postives = 179/221 (81.00%), Query Frame = 0


Query: 1   MSFEISSPIPYLLNANGFGNGVLEVQLSYLERAISVSSFSQRILYETQPTPFNNALFYLS 60
           MSF ISSPI YLLN  GFGNGVLE+QLSY+ER IS+SS SQRILYETQP PFNNA FYLS
Sbjct: 1   MSFAISSPIQYLLNGTGFGNGVLEIQLSYVERVISISSVSQRILYETQPIPFNNAHFYLS 60

Query: 61  VHQLQHPLFHIRQFLYSLNIRRETIARQIASYVAHMCNGNTGWNSNFYVIARVDLVRVIR 120
           VH LQ PLF+IRQFL+SLNIRRE IARQIASYVAHMCNGNTG NSNFYVIARVDLVR IR
Sbjct: 61  VHHLQDPLFYIRQFLFSLNIRRENIARQIASYVAHMCNGNTGRNSNFYVIARVDLVRAIR 120

Query: 121 IEEQ---------------------PRAEGWRGVAV-ERLSKLKSEEEKGDCSVCLDELD 180
           IEE+                     PRA   RGV V ERL KLKSEEE+GDCSVCLDELD
Sbjct: 121 IEEESMWRDGGGVVVEAETVVFDEVPRAR--RGVVVGERLRKLKSEEEEGDCSVCLDELD 180

Query: 181 CEKREVIRIPCGHVYHESCIFKWLNSSNSCPLCRSSFPLHN 200
           C KREVIRIPCGHVYHESCIF+WLN++NSCPLCR+SFPL N
Sbjct: 181 CGKREVIRIPCGHVYHESCIFEWLNNNNSCPLCRTSFPLDN 219

[Close]

BLAST of CSPI04G01210.1 vs. ExPASy TrEMBL
Match: A0A1S4DZR1 (uncharacterized protein LOC107991269 OS=Cucumis melo OX=3656 GN=LOC107991269 PE=4 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 1.2e-85
Identity = 168/221 (76.02%), Postives = 179/221 (81.00%), Query Frame = 0


Query: 1   MSFEISSPIPYLLNANGFGNGVLEVQLSYLERAISVSSFSQRILYETQPTPFNNALFYLS 60
           MSF ISSPI YLLN  GFGNGVLE+QLSY+ER IS+SS SQRILYETQP PFNNA FYLS
Sbjct: 1   MSFAISSPIQYLLNGTGFGNGVLEIQLSYVERVISISSVSQRILYETQPIPFNNAHFYLS 60

Query: 61  VHQLQHPLFHIRQFLYSLNIRRETIARQIASYVAHMCNGNTGWNSNFYVIARVDLVRVIR 120
           VH LQ PLF+IRQFL+SLNIRRE IARQIASYVAHMCNGNTG NSNFYVIARVDLVR IR
Sbjct: 61  VHHLQDPLFYIRQFLFSLNIRRENIARQIASYVAHMCNGNTGRNSNFYVIARVDLVRAIR 120

Query: 121 IEEQ---------------------PRAEGWRGVAV-ERLSKLKSEEEKGDCSVCLDELD 180
           IEE+                     PRA   RGV V ERL KLKSEEE+GDCSVCLDELD
Sbjct: 121 IEEESMWRDGGGVVVEAETVVFDEVPRAR--RGVVVGERLRKLKSEEEEGDCSVCLDELD 180

Query: 181 CEKREVIRIPCGHVYHESCIFKWLNSSNSCPLCRSSFPLHN 200
           C KREVIRIPCGHVYHESCIF+WLN++NSCPLCR+SFPL N
Sbjct: 181 CGKREVIRIPCGHVYHESCIFEWLNNNNSCPLCRTSFPLDN 219

[Close]

BLAST of CSPI04G01210.1 vs. ExPASy TrEMBL
Match: A0A5D3E1S5 (E3 ubiquitin-protein ligase RNF181-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold208G00800 PE=4 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 1.9e-49
Identity = 118/223 (52.91%), Postives = 149/223 (66.82%), Query Frame = 0


Query: 1   MSFEISSPIPYLLNANGF-GNGVLEVQLSYLERAISVSSFSQR---ILYETQPTPFNNAL 60
           MSF IS+PI Y L  +G  GNG+LEV++S+ E+ IS+S  + R   IL++T   P++NAL
Sbjct: 1   MSFAISNPIQYRLYGSGTGGNGILEVRISFFEQMISLSPVTGRPHQILHQTPRIPYHNAL 60

Query: 61  FYLSVHQLQHPLFHIRQFLYSLNIR---RETIARQIASYVAHMCNGNTGWNSNFYVIARV 120
            YL++ QLQ PLF+I QFL SLNIR    +++ARQIAS++  M + N   + NF++IA V
Sbjct: 61  VYLTLRQLQSPLFYITQFLSSLNIRPAASQSMARQIASHILQMPDRN--GDRNFHIIAEV 120

Query: 121 DLVRVI--RIEEQPRAEG--------------WRGVAVERLSKLKSE---EEKGDCSVCL 180
           D +RVI   +EEQ    G               RGV   R  +LKSE   E+ GDCS+CL
Sbjct: 121 DFIRVIWLELEEQSMGAGAEETVVFDEAPPALKRGVGKARGERLKSEEKVEDLGDCSICL 180

Query: 181 DELDCEKREVIRIPCGHVYHESCIFKWLNSSNSCPLCRSSFPL 198
           DEL CEKREVIRIPCGHVYHESCIFKWL + NSCPLCR   PL
Sbjct: 181 DELSCEKREVIRIPCGHVYHESCIFKWLKNHNSCPLCRKPLPL 221

[Close]

BLAST of CSPI04G01210.1 vs. ExPASy TrEMBL
Match: A0A1S4DUZ4 (E3 ubiquitin-protein ligase RNF181-like OS=Cucumis melo OX=3656 GN=LOC107990564 PE=4 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 1.9e-49
Identity = 118/223 (52.91%), Postives = 149/223 (66.82%), Query Frame = 0


Query: 1   MSFEISSPIPYLLNANGF-GNGVLEVQLSYLERAISVSSFSQR---ILYETQPTPFNNAL 60
           MSF IS+PI Y L  +G  GNG+LEV++S+ E+ IS+S  + R   IL++T   P++NAL
Sbjct: 1   MSFAISNPIQYRLYGSGTGGNGILEVRISFFEQMISLSPVTGRPHQILHQTPRIPYHNAL 60

Query: 61  FYLSVHQLQHPLFHIRQFLYSLNIR---RETIARQIASYVAHMCNGNTGWNSNFYVIARV 120
            YL++ QLQ PLF+I QFL SLNIR    +++ARQIAS++  M + N   + NF++IA V
Sbjct: 61  VYLTLRQLQSPLFYITQFLSSLNIRPAASQSMARQIASHILQMPDRN--GDRNFHIIAEV 120

Query: 121 DLVRVI--RIEEQPRAEG--------------WRGVAVERLSKLKSE---EEKGDCSVCL 180
           D +RVI   +EEQ    G               RGV   R  +LKSE   E+ GDCS+CL
Sbjct: 121 DFIRVIWLELEEQSMGAGAEETVVFDEAPPALKRGVGKARGERLKSEEKVEDLGDCSICL 180

Query: 181 DELDCEKREVIRIPCGHVYHESCIFKWLNSSNSCPLCRSSFPL 198
           DEL CEKREVIRIPCGHVYHESCIFKWL + NSCPLCR   PL
Sbjct: 181 DELSCEKREVIRIPCGHVYHESCIFKWLKNHNSCPLCRKPLPL 221

[Close]

BLAST of CSPI04G01210.1 vs. NCBI nr
Match: KGN52856.2 (hypothetical protein Csa_014722 [Cucumis sativus])

HSP 1 Score: 377.1 bits (967), Expect = 9.6e-101
Identity = 183/184 (99.46%), Postives = 184/184 (100.00%), Query Frame = 0


Query: 1   MSFEISSPIPYLLNANGFGNGVLEVQLSYLERAISVSSFSQRILYETQPTPFNNALFYLS 60
           MSFEISSPIPYLLNANGFGNGVLEVQLSYLERAISVSSFSQRILYETQPTPFNNALFYLS
Sbjct: 1   MSFEISSPIPYLLNANGFGNGVLEVQLSYLERAISVSSFSQRILYETQPTPFNNALFYLS 60

Query: 61  VHQLQHPLFHIRQFLYSLNIRRETIARQIASYVAHMCNGNTGWNSNFYVIARVDLVRVIR 120
           VHQLQHPLFHIRQFLYSLNIRRETIARQIAS+VAHMCNGNTGWNSNFYVIARVDLVRVIR
Sbjct: 61  VHQLQHPLFHIRQFLYSLNIRRETIARQIASFVAHMCNGNTGWNSNFYVIARVDLVRVIR 120

Query: 121 IEEQPRAEGWRGVAVERLSKLKSEEEKGDCSVCLDELDCEKREVIRIPCGHVYHESCIFK 180
           IEEQPRAEGWRGVAVERLSKLKSEEEKGDCSVCLDELDCEKREVIRIPCGHVYHESCIFK
Sbjct: 121 IEEQPRAEGWRGVAVERLSKLKSEEEKGDCSVCLDELDCEKREVIRIPCGHVYHESCIFK 180

Query: 181 WLNS 185
           WLNS
Sbjct: 181 WLNS 184

[Close]

BLAST of CSPI04G01210.1 vs. NCBI nr
Match: XP_016901482.1 (PREDICTED: uncharacterized protein LOC107991269 [Cucumis melo] >KAA0044413.1 E3 ubiquitin-protein ligase RNF181-like protein [Cucumis melo var. makuwa] >TYK29540.1 E3 ubiquitin-protein ligase RNF181-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 325.9 bits (834), Expect = 2.5e-85
Identity = 168/221 (76.02%), Postives = 179/221 (81.00%), Query Frame = 0


Query: 1   MSFEISSPIPYLLNANGFGNGVLEVQLSYLERAISVSSFSQRILYETQPTPFNNALFYLS 60
           MSF ISSPI YLLN  GFGNGVLE+QLSY+ER IS+SS SQRILYETQP PFNNA FYLS
Sbjct: 1   MSFAISSPIQYLLNGTGFGNGVLEIQLSYVERVISISSVSQRILYETQPIPFNNAHFYLS 60

Query: 61  VHQLQHPLFHIRQFLYSLNIRRETIARQIASYVAHMCNGNTGWNSNFYVIARVDLVRVIR 120
           VH LQ PLF+IRQFL+SLNIRRE IARQIASYVAHMCNGNTG NSNFYVIARVDLVR IR
Sbjct: 61  VHHLQDPLFYIRQFLFSLNIRRENIARQIASYVAHMCNGNTGRNSNFYVIARVDLVRAIR 120

Query: 121 IEEQ---------------------PRAEGWRGVAV-ERLSKLKSEEEKGDCSVCLDELD 180
           IEE+                     PRA   RGV V ERL KLKSEEE+GDCSVCLDELD
Sbjct: 121 IEEESMWRDGGGVVVEAETVVFDEVPRAR--RGVVVGERLRKLKSEEEEGDCSVCLDELD 180

Query: 181 CEKREVIRIPCGHVYHESCIFKWLNSSNSCPLCRSSFPLHN 200
           C KREVIRIPCGHVYHESCIF+WLN++NSCPLCR+SFPL N
Sbjct: 181 CGKREVIRIPCGHVYHESCIFEWLNNNNSCPLCRTSFPLDN 219

[Close]

BLAST of CSPI04G01210.1 vs. NCBI nr
Match: XP_038883574.1 (E3 ubiquitin-protein ligase RNF181-like [Benincasa hispida])

HSP 1 Score: 223.8 bits (569), Expect = 1.4e-54
Identity = 127/226 (56.19%), Postives = 148/226 (65.49%), Query Frame = 0


Query: 1   MSFEISSPIPYLL--NANGFGNGVLEVQLSYLERAISVSSFS---QRILYETQPTPFNNA 60
           MS   S+PI Y L    +G GNGVLEVQ SY ER ISVS  +    RIL+ET   PF+NA
Sbjct: 1   MSSAASTPIQYRLYGTGSGTGNGVLEVQTSYFERMISVSPVTGRPPRILHETPRIPFHNA 60

Query: 61  LFYLSVHQLQHPLFHIRQFLYSLNI---RRETIARQIASYVAHMCNGNTGWNSNFYVIAR 120
           LFYLS+HQLQ+PLF+I QFL SLNI     E +ARQIASY+  M N N    SNF +IA 
Sbjct: 61  LFYLSLHQLQNPLFYISQFLSSLNICPAACEIMARQIASYIDRMINENR--ESNFRIIAE 120

Query: 121 VDLVRVIRIEEQ-------------------PRAEGWRGVAVERLSKLKSEEEKGDCSVC 180
           VD  RVI +EE                    P   G   VAVER+ +LKS+EE GDC +C
Sbjct: 121 VDFSRVIWMEEHSIYGGDAMAEETVVFDEAPPPRRGVEKVAVERVRELKSQEELGDCCIC 180

Query: 181 LDELDCEKREVIRIPCGHVYHESCIFKWLNSSNSCPLCRSSFPLHN 200
           L+EL CEKREVIRIPCGHVYHESC+FKWL  +NSCPLCR   PL++
Sbjct: 181 LEELSCEKREVIRIPCGHVYHESCVFKWLRKNNSCPLCRKPLPLND 224

[Close]

BLAST of CSPI04G01210.1 vs. NCBI nr
Match: XP_016899530.1 (PREDICTED: E3 ubiquitin-protein ligase RNF181-like [Cucumis melo] >KAA0035774.1 E3 ubiquitin-protein ligase RNF181-like protein [Cucumis melo var. makuwa] >TYK29842.1 E3 ubiquitin-protein ligase RNF181-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 205.7 bits (522), Expect = 3.8e-49
Identity = 118/223 (52.91%), Postives = 149/223 (66.82%), Query Frame = 0


Query: 1   MSFEISSPIPYLLNANGF-GNGVLEVQLSYLERAISVSSFSQR---ILYETQPTPFNNAL 60
           MSF IS+PI Y L  +G  GNG+LEV++S+ E+ IS+S  + R   IL++T   P++NAL
Sbjct: 1   MSFAISNPIQYRLYGSGTGGNGILEVRISFFEQMISLSPVTGRPHQILHQTPRIPYHNAL 60

Query: 61  FYLSVHQLQHPLFHIRQFLYSLNIR---RETIARQIASYVAHMCNGNTGWNSNFYVIARV 120
            YL++ QLQ PLF+I QFL SLNIR    +++ARQIAS++  M + N   + NF++IA V
Sbjct: 61  VYLTLRQLQSPLFYITQFLSSLNIRPAASQSMARQIASHILQMPDRN--GDRNFHIIAEV 120

Query: 121 DLVRVI--RIEEQPRAEG--------------WRGVAVERLSKLKSE---EEKGDCSVCL 180
           D +RVI   +EEQ    G               RGV   R  +LKSE   E+ GDCS+CL
Sbjct: 121 DFIRVIWLELEEQSMGAGAEETVVFDEAPPALKRGVGKARGERLKSEEKVEDLGDCSICL 180

Query: 181 DELDCEKREVIRIPCGHVYHESCIFKWLNSSNSCPLCRSSFPL 198
           DEL CEKREVIRIPCGHVYHESCIFKWL + NSCPLCR   PL
Sbjct: 181 DELSCEKREVIRIPCGHVYHESCIFKWLKNHNSCPLCRKPLPL 221

[Close]

BLAST of CSPI04G01210.1 vs. NCBI nr
Match: XP_004144954.1 (uncharacterized protein LOC101214496 [Cucumis sativus] >KGN43349.1 hypothetical protein Csa_020356 [Cucumis sativus])

HSP 1 Score: 203.0 bits (515), Expect = 2.5e-48
Identity = 116/225 (51.56%), Postives = 153/225 (68.00%), Query Frame = 0


Query: 1   MSFEISSPIPYLLNANGF-GNGVLEVQLSYLERAISVSSFSQR---ILYETQPTPFNNAL 60
           MSF IS+PI Y L A+G  GNGVL+V +S++++ ISVS  + R   +L++T   P++NAL
Sbjct: 1   MSFAISNPIQYRLYASGTGGNGVLQVNISFIQQIISVSPLTGRPHEVLHQTPRIPYHNAL 60

Query: 61  FYLSVHQLQHPLFHIRQFLYSLNIR---RETIARQIASYVAHMCNGNTGWNSNFYVIARV 120
           FYLS+ QLQ PLF+I Q L SLNIR    + +AR+IAS++  M + N+   +NF+++A V
Sbjct: 61  FYLSLRQLQSPLFYITQILSSLNIRPAASQNMARRIASHILQMPDRNS--ETNFHILAEV 120

Query: 121 DLVRVI----------------RIEEQPRAEGWRGVAVERLSKLKSE---EEKGDCSVCL 180
           D +R+I                 ++E P A   RGV V R  +L+SE   EE GDCS+CL
Sbjct: 121 DFIRLIWLPSMGGGGAGAEETVVLDEAPPAVK-RGVGVARGERLRSEEKMEELGDCSICL 180

Query: 181 DELDCEKREVIRIPCGHVYHESCIFKWLNSSNSCPLCRSSFPLHN 200
           DEL CEKREV+RIPCGHVYHESCIFKWL + NSCPLCR   PLH+
Sbjct: 181 DELSCEKREVMRIPCGHVYHESCIFKWLENHNSCPLCRK--PLHH 220

[Close]

BLAST of CSPI04G01210.1 vs. TAIR 10
Match: AT5G37200.1 (RING/U-box superfamily protein )

HSP 1 Score: 64.7 bits (156), Expect = 9.8e-11
Identity = 26/63 (41.27%), Postives = 40/63 (63.49%), Query Frame = 0


Query: 131 RGVAVERLSKLKSEEEKGDCSVCLDELDCEKREVIRIP-CGHVYHESCIFKWLNSSNSCP 190
           R V  +R+     EEE   CS+C+++      ++IR+P C H++H+ C+F+WL   NSCP
Sbjct: 140 RLVEEQRVESADLEEEDETCSICIEKFSESHEDIIRVPDCLHLFHQGCLFEWLGLQNSCP 199

Query: 191 LCR 193
           LCR
Sbjct: 200 LCR 202

[Close]

BLAST of CSPI04G01210.1 vs. TAIR 10
Match: AT4G12190.1 (RING/U-box superfamily protein )

HSP 1 Score: 60.8 bits (146), Expect = 1.4e-09
Identity = 26/63 (41.27%), Postives = 38/63 (60.32%), Query Frame = 0


Query: 133 VAVERLSKLKSEEEKGDCSVCLDEL--DCEKREVIRIPCGHVYHESCIFKWLNSSNSCPL 192
           +  E L  LK E E   CS+CL+ L    + R++ R+ C HV+H  C+ +WL   N+CPL
Sbjct: 7   IKTEELKSLKMETE--PCSICLESLVSGPKPRDITRMTCSHVFHNGCLLEWLKRKNTCPL 66

Query: 193 CRS 194
           CR+
Sbjct: 67  CRT 67

[Close]

BLAST of CSPI04G01210.1 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 60.1 bits (144), Expect = 2.4e-09
Identity = 23/60 (38.33%), Postives = 38/60 (63.33%), Query Frame = 0


Query: 137 RLSKLKSEEEKGDCSVCLDELDCEKREVIRIPCGHVYHESCIFKWLNSSNSCPLCRSSFP 196
           +++K   + E   C+VC+DE + +  +V ++PC HV+H+ C+  WL   NSCP+CR   P
Sbjct: 203 KVTKDMLKSEMNQCAVCMDEFE-DGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELP 261

[Close]

BLAST of CSPI04G01210.1 vs. TAIR 10
Match: AT1G35330.1 (RING/U-box superfamily protein )

HSP 1 Score: 59.3 bits (142), Expect = 4.1e-09
Identity = 21/48 (43.75%), Postives = 34/48 (70.83%), Query Frame = 0


Query: 149 DCSVCLDELDCEKREVIRIPCGHVYHESCIFKWLNSSNSCPLCRSSFP 197
           +C++CL+E + E+   +  PC H +H SCI  WL+S ++CP+CR+S P
Sbjct: 127 ECAICLNEFEDEETLRLMPPCSHAFHASCIDVWLSSRSTCPVCRASLP 174

[Close]

BLAST of CSPI04G01210.1 vs. TAIR 10
Match: AT2G44330.1 (RING/U-box superfamily protein )

HSP 1 Score: 58.9 bits (141), Expect = 5.4e-09
Identity = 24/65 (36.92%), Postives = 35/65 (53.85%), Query Frame = 0


Query: 133 VAVERLSKLKSEEEKGDCSVCLDELDCEKREVIRIPCGHVYHESCIFKWLNSSNSCPLCR 192
           ++   LS   S++    C++C ++         R+PC H+YH  CI  WL S NSCPLCR
Sbjct: 79  ISSSMLSSASSDDSALPCAICREDF-VVGESARRLPCNHLYHNDCIIPWLTSHNSCPLCR 138

Query: 193 SSFPL 198
              P+
Sbjct: 139 VELPV 142

The following BLAST results are available for this feature:

BLAST of CSPI04G01210.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9CY62	9.0e-09	45.31	E3 ubiquitin-protein ligase RNF181 OS=Mus musculus OX=10090 GN=Rnf181 PE=1 SV=1	[more]
Q3T0W3	1.2e-08	40.00	E3 ubiquitin-protein ligase RNF181 OS=Bos taurus OX=9913 GN=RNF181 PE=2 SV=1	[more]
Q6AXU4	1.2e-08	44.64	E3 ubiquitin-protein ligase RNF181 OS=Rattus norvegicus OX=10116 GN=Rnf181 PE=1 ...	[more]
Q5M974	1.2e-08	46.27	E3 ubiquitin-protein ligase RNF181 OS=Xenopus tropicalis OX=8364 GN=rnf181 PE=2 ...	[more]
Q9P0P0	2.0e-08	40.58	E3 ubiquitin-protein ligase RNF181 OS=Homo sapiens OX=9606 GN=RNF181 PE=1 SV=1	[more]

BLAST of CSPI04G01210.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KYC0	6.9e-113	99.50	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G003650 P...	[more]
A0A5A7TLM1	1.2e-85	76.02	E3 ubiquitin-protein ligase RNF181-like protein OS=Cucumis melo var. makuwa OX=1...	[more]
A0A1S4DZR1	1.2e-85	76.02	uncharacterized protein LOC107991269 OS=Cucumis melo OX=3656 GN=LOC107991269 PE=...	[more]
A0A5D3E1S5	1.9e-49	52.91	E3 ubiquitin-protein ligase RNF181-like protein OS=Cucumis melo var. makuwa OX=1...	[more]
A0A1S4DUZ4	1.9e-49	52.91	E3 ubiquitin-protein ligase RNF181-like OS=Cucumis melo OX=3656 GN=LOC107990564 ...	[more]

BLAST of CSPI04G01210.1 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN52856.2	9.6e-101	99.46	hypothetical protein Csa_014722 [Cucumis sativus]	[more]
XP_016901482.1	2.5e-85	76.02	PREDICTED: uncharacterized protein LOC107991269 [Cucumis melo] >KAA0044413.1 E3 ...	[more]
XP_038883574.1	1.4e-54	56.19	E3 ubiquitin-protein ligase RNF181-like [Benincasa hispida]	[more]
XP_016899530.1	3.8e-49	52.91	PREDICTED: E3 ubiquitin-protein ligase RNF181-like [Cucumis melo] >KAA0035774.1 ...	[more]
XP_004144954.1	2.5e-48	51.56	uncharacterized protein LOC101214496 [Cucumis sativus] >KGN43349.1 hypothetical ...	[more]

BLAST of CSPI04G01210.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G37200.1	9.8e-11	41.27	RING/U-box superfamily protein	[more]
AT4G12190.1	1.4e-09	41.27	RING/U-box superfamily protein	[more]
AT3G19950.1	2.4e-09	38.33	RING/U-box superfamily protein	[more]
AT1G35330.1	4.1e-09	43.75	RING/U-box superfamily protein	[more]
AT2G44330.1	5.4e-09	36.92	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 150..191 e-value: 4.6E-8 score: 42.8
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 149..192 e-value: 4.3E-13 score: 49.4
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 150..192 score: 13.551494
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 120..197 e-value: 9.4E-19 score: 68.9
None	No IPR available	PANTHER	PTHR22765:SF329	SUBFAMILY NOT NAMED	coord: 4..193
None	No IPR available	PANTHER	PTHR22765	RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAINING	coord: 4..193
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 149..197

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI04G01210	CSPI04G01210	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI04G01210.1.cds1	CSPI04G01210.1.cds1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI04G01210.1	CSPI04G01210.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0031507	heterochromatin assembly
biological_process	GO:0070734	histone H3-K27 methylation
biological_process	GO:0048587	regulation of short-day photoperiodism, flowering
cellular_component	GO:0005677	chromatin silencing complex
cellular_component	GO:0031519	PcG protein complex
molecular_function	GO:0008168	methyltransferase activity