Bhi12M000377 (mRNA) Wax gourd (B227) v1

Overview


Name	Bhi12M000377
Type	mRNA
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Vacuolar-processing enzyme
Location	chr12: 13490117 .. 13490706 (-)
Sequence length	395
RNA-Seq Expression	Bhi12M000377
Synteny	Bhi12M000377

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GATTACATTGGGGAGCATGTGACTGCTCAGACTGTTCTGTGTGCTTTCTGGGAAATATAACTGCTGTTAATAGAGGGAGTGGGAAAGTTGTGGACAGCAAACGAAATGACAGGATTTTTGTTTACTATTCTGACATGGAGGCCCTGGGTACTTGGTTTGTTTTCAAGAACAAGACTAGTTGAATTGATTTCATTTGAATTTTGTTGAACTAAAGCTAGTACATATACTGTTTTCCAGGCATGCCAACTTGCCTTTTGTCTATGCCATGGACTTTTATTGAAGTTTTAAAGAAGAAACATGCTGCTAAGGAATACAAAGAGATGGTAAAGAAGAGAGAGATAGAGAGATCAAATTTCTAAGCACTGTGAAAAACTTTTGTAATGTAATGTTACATGGTGTAAACATTTTTGTAATGTTGCATTTTGTAGGTTATCTATGTAGAAGCATGTGAGAGTGGGAGTATTTTCGAGGGCATAATGCCAAAGGATCTGAATATTTATGCAACCCACAGCCTCAAATGCTCAAGAGAAGTAGGCTTTTGGAACTTACTGCCCAGGAATGCAACCGGCACCGCCACCGGAGATACATGA

mRNA sequence

GATTACATTGGGGAGCATGTGACTGCTCAGACTGTTCTGTGTGCTTTCTGGGAAATATAACTGCTGTTAATAGAGGGAGTGGGAAAGTTGTGGACAGCAAACGAAATGACAGGATTTTTGTTTACTATTCTGACATGGAGGCCCTGGGCATGCCAACTTGCCTTTTGTCTATGCCATGGACTTTTATTGAAGTTTTAAAGAAGAAACATGCTGCTAAGGAATACAAAGAGATGGTTATCTATGTAGAAGCATGTGAGAGTGGGAGTATTTTCGAGGGCATAATGCCAAAGGATCTGAATATTTATGCAACCCACAGCCTCAAATGCTCAAGAGAAGTAGGCTTTTGGAACTTACTGCCCAGGAATGCAACCGGCACCGCCACCGGAGATACATGA

Coding sequence (CDS)

GATTACATTGGGGAGCATGTGACTGCTCAGACTGTTCTGTGTGCTTTCTGGGAAATATAACTGCTGTTAATAGAGGGAGTGGGAAAGTTGTGGACAGCAAACGAAATGACAGGATTTTTGTTTACTATTCTGACATGGAGGCCCTGGGCATGCCAACTTGCCTTTTGTCTATGCCATGGACTTTTATTGAAGTTTTAAAGAAGAAACATGCTGCTAAGGAATACAAAGAGATGGTTATCTATGTAGAAGCATGTGAGAGTGGGAGTATTTTCGAGGGCATAATGCCAAAGGATCTGAATATTTATGCAACCCACAGCCTCAAATGCTCAAGAGAAGTAGGCTTTTGGAACTTACTGCCCAGGAATGCAACCGGCACCGCCACCGGAGATACATGA

Protein sequence

LHWGACDCSDCSVCFLGNITAVNRGSGKVVDSKRNDRIFVYYSDMEALGMPTCLLSMPWTFIEVLKKKHAAKEYKEMVIYVEACESGSIFEGIMPKDLNIYATHSLKCSREVGFWNLLPRNATGTATGDT

Homology

[Close]

BLAST of Bhi12M000377 vs. TAIR 10
Match: AT1G62710.1 (beta vacuolar processing enzyme )

HSP 1 Score: 114.4 bits (285), Expect = 6.9e-26
Identity = 61/91 (67.03%), Postives = 65/91 (71.43%), Query Frame = 0


Query: 16  LGNITAVNRGSGKVVDSKRNDRIFVYYSD---MEALGMPTCLLSMPWTFIEVLKKKHAAK 75
           LG+  AV  GSGKV+ SK ND IFVYY+D      LGMP         FIE LKKKHA+ 
Sbjct: 140 LGDQKAVKGGSGKVIASKPNDHIFVYYADHGGPGVLGMPNTPHIYAADFIETLKKKHASG 199

Query: 76  EYKEMVIYVEACESGSIFEGIMPKDLNIYAT 104
            YKEMVIYVEACESGSIFEGIMPKDLNIY T
Sbjct: 200 TYKEMVIYVEACESGSIFEGIMPKDLNIYVT 230

[Close]

BLAST of Bhi12M000377 vs. TAIR 10
Match: AT2G25940.1 (alpha-vacuolar processing enzyme )

HSP 1 Score: 103.2 bits (256), Expect = 1.6e-22
Identity = 54/91 (59.34%), Postives = 64/91 (70.33%), Query Frame = 0


Query: 16  LGNITAVNRGSGKVVDSKRNDRIFVYYSD---MEALGMPTCLLSMPWTFIEVLKKKHAAK 75
           LGN TA+  GSGKVVDS  ND IF+YYSD      LGMPT          +VLKKK+A+ 
Sbjct: 133 LGNKTALKGGSGKVVDSGPNDHIFIYYSDHGGPGVLGMPTSPNLYANDLNDVLKKKYASG 192

Query: 76  EYKEMVIYVEACESGSIFEGIMPKDLNIYAT 104
            YK +V Y+EACESGSIFEG++P+ LNIYAT
Sbjct: 193 TYKSLVFYLEACESGSIFEGLLPEGLNIYAT 223

[Close]

BLAST of Bhi12M000377 vs. TAIR 10
Match: AT4G32940.1 (gamma vacuolar processing enzyme )

HSP 1 Score: 103.2 bits (256), Expect = 1.6e-22
Identity = 54/91 (59.34%), Postives = 63/91 (69.23%), Query Frame = 0


Query: 16  LGNITAVNRGSGKVVDSKRNDRIFVYYSD---MEALGMPTCLLSMPWTFIEVLKKKHAAK 75
           LG+ TAV  GSGKVVDS  ND IF++YSD      LGMPT          +VLKKKHA  
Sbjct: 148 LGDKTAVKGGSGKVVDSGPNDHIFIFYSDHGGPGVLGMPTSPYLYANDLNDVLKKKHALG 207

Query: 76  EYKEMVIYVEACESGSIFEGIMPKDLNIYAT 104
            YK +V Y+EACESGSIFEG++P+ LNIYAT
Sbjct: 208 TYKSLVFYLEACESGSIFEGLLPEGLNIYAT 238

[Close]

BLAST of Bhi12M000377 vs. TAIR 10
Match: AT3G20210.1 (delta vacuolar processing enzyme )

HSP 1 Score: 101.7 bits (252), Expect = 4.6e-22
Identity = 60/125 (48.00%), Postives = 75/125 (60.00%), Query Frame = 0


Query: 16  LGNITAVNRGSGKVVDSKRNDRIFVYYSDMEALG---MPTCLLSMPWTFIEVLKKKHAAK 75
           LGN + V  G+GKVV S  ND IF+YY+D  A G   MPT    M   F EVL+K H  K
Sbjct: 135 LGNESGVTGGNGKVVKSGPNDNIFIYYADHGAPGLIAMPTGDEVMAKDFNEVLEKMHKRK 194

Query: 76  EYKEMVIYVEACESGSIFEGIMPKDLNIYATHSLKCSREVGFWNLL-------PRNATGT 131
           +Y +MVIYVEACESGS+FEGI+ K+LNIYA  +   + +   W +        P +  GT
Sbjct: 195 KYNKMVIYVEACESGSMFEGILKKNLNIYAVTA--ANSKESSWGVYCPESYPPPPSEIGT 254

[Close]

BLAST of Bhi12M000377 vs. TAIR 10
Match: AT3G20210.2 (delta vacuolar processing enzyme )

HSP 1 Score: 101.7 bits (252), Expect = 4.6e-22
Identity = 60/125 (48.00%), Postives = 75/125 (60.00%), Query Frame = 0


Query: 16  LGNITAVNRGSGKVVDSKRNDRIFVYYSDMEALG---MPTCLLSMPWTFIEVLKKKHAAK 75
           LGN + V  G+GKVV S  ND IF+YY+D  A G   MPT    M   F EVL+K H  K
Sbjct: 135 LGNESGVTGGNGKVVKSGPNDNIFIYYADHGAPGLIAMPTGDEVMAKDFNEVLEKMHKRK 194

Query: 76  EYKEMVIYVEACESGSIFEGIMPKDLNIYATHSLKCSREVGFWNLL-------PRNATGT 131
           +Y +MVIYVEACESGS+FEGI+ K+LNIYA  +   + +   W +        P +  GT
Sbjct: 195 KYNKMVIYVEACESGSMFEGILKKNLNIYAVTA--ANSKESSWGVYCPESYPPPPSEIGT 254

[Close]

BLAST of Bhi12M000377 vs. ExPASy Swiss-Prot
Match: P49042 (Vacuolar-processing enzyme OS=Ricinus communis OX=3988 PE=1 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 2.3e-26
Identity = 63/91 (69.23%), Postives = 69/91 (75.82%), Query Frame = 0


Query: 16  LGNITAVNRGSGKVVDSKRNDRIFVYYSD---MEALGMPTCLLSMPWTFIEVLKKKHAAK 75
           LG+ +AV  GSGKVVDSK NDRIF+YYSD      LGMP         FIEVLKKKHAA 
Sbjct: 151 LGDKSAVQGGSGKVVDSKPNDRIFLYYSDHGGPGVLGMPNLPYLYAMDFIEVLKKKHAAG 210

Query: 76  EYKEMVIYVEACESGSIFEGIMPKDLNIYAT 104
            YK+MVIYVEACESGSIFEGIMPKD++IY T
Sbjct: 211 GYKKMVIYVEACESGSIFEGIMPKDVDIYVT 241

[Close]

BLAST of Bhi12M000377 vs. ExPASy Swiss-Prot
Match: O24326 (Vacuolar-processing enzyme OS=Phaseolus vulgaris OX=3885 PE=2 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 2.0e-25
Identity = 61/91 (67.03%), Postives = 67/91 (73.63%), Query Frame = 0


Query: 16  LGNITAVNRGSGKVVDSKRNDRIFVYYSD---MEALGMPTCLLSMPWTFIEVLKKKHAAK 75
           LG+ + V  GSGKV++SK  DRIFVYYSD      LGMP         FI+VLKKKHA+ 
Sbjct: 147 LGDKSKVKGGSGKVINSKPEDRIFVYYSDHGGPGVLGMPNMPYLYAMDFIDVLKKKHASG 206

Query: 76  EYKEMVIYVEACESGSIFEGIMPKDLNIYAT 104
            YKEMVIYVEACESGSIFEGIMPKDLNIY T
Sbjct: 207 GYKEMVIYVEACESGSIFEGIMPKDLNIYVT 237

[Close]

BLAST of Bhi12M000377 vs. ExPASy Swiss-Prot
Match: P49045 (Vacuolar-processing enzyme OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 4.4e-25
Identity = 58/91 (63.74%), Postives = 67/91 (73.63%), Query Frame = 0


Query: 16  LGNITAVNRGSGKVVDSKRNDRIFVYYSDMEA---LGMPTCLLSMPWTFIEVLKKKHAAK 75
           LG+ + +  GSGKV++SK  DRIF+YYSD      LGMP         FI+VLKKKHA+ 
Sbjct: 149 LGDKSKLKGGSGKVINSKPEDRIFIYYSDHGGPGILGMPNMPYLYAMDFIDVLKKKHASG 208

Query: 76  EYKEMVIYVEACESGSIFEGIMPKDLNIYAT 104
            YKEMVIYVEACESGS+FEGIMPKDLNIY T
Sbjct: 209 SYKEMVIYVEACESGSVFEGIMPKDLNIYVT 239

[Close]

BLAST of Bhi12M000377 vs. ExPASy Swiss-Prot
Match: Q39044 (Vacuolar-processing enzyme beta-isozyme OS=Arabidopsis thaliana OX=3702 GN=bVPE PE=1 SV=3)

HSP 1 Score: 114.4 bits (285), Expect = 9.7e-25
Identity = 61/91 (67.03%), Postives = 65/91 (71.43%), Query Frame = 0


Query: 16  LGNITAVNRGSGKVVDSKRNDRIFVYYSD---MEALGMPTCLLSMPWTFIEVLKKKHAAK 75
           LG+  AV  GSGKV+ SK ND IFVYY+D      LGMP         FIE LKKKHA+ 
Sbjct: 140 LGDQKAVKGGSGKVIASKPNDHIFVYYADHGGPGVLGMPNTPHIYAADFIETLKKKHASG 199

Query: 76  EYKEMVIYVEACESGSIFEGIMPKDLNIYAT 104
            YKEMVIYVEACESGSIFEGIMPKDLNIY T
Sbjct: 200 TYKEMVIYVEACESGSIFEGIMPKDLNIYVT 230

[Close]

BLAST of Bhi12M000377 vs. ExPASy Swiss-Prot
Match: P49046 (Legumain OS=Canavalia ensiformis OX=3823 PE=1 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 2.2e-24
Identity = 57/91 (62.64%), Postives = 66/91 (72.53%), Query Frame = 0


Query: 16  LGNITAVNRGSGKVVDSKRNDRIFVYYSD---MEALGMPTCLLSMPWTFIEVLKKKHAAK 75
           LG+ + V  GSGKV++S   DRIF++YSD      LGMP         FI+VLKKKHA+ 
Sbjct: 129 LGDKSKVKGGSGKVINSNPEDRIFIFYSDHGGPGVLGMPNAPFVYAMDFIDVLKKKHASG 188

Query: 76  EYKEMVIYVEACESGSIFEGIMPKDLNIYAT 104
            YKEMVIY+EACESGSIFEGIMPKDLNIY T
Sbjct: 189 GYKEMVIYIEACESGSIFEGIMPKDLNIYVT 219

[Close]

BLAST of Bhi12M000377 vs. NCBI nr
Match: XP_008437131.1 (PREDICTED: legumain [Cucumis melo])

HSP 1 Score: 134.4 bits (337), Expect = 6.9e-28
Identity = 71/91 (78.02%), Postives = 71/91 (78.02%), Query Frame = 0


Query: 16  LGNITAVNRGSGKVVDSKRNDRIFVYYSD---MEALGMPTCLLSMPWTFIEVLKKKHAAK 75
           LGN TAVN GSGKVVDSK NDRIFVYYSD      LGMP         FIEVLKKKHAAK
Sbjct: 147 LGNRTAVNGGSGKVVDSKPNDRIFVYYSDHGGPGVLGMPNLPFVYAMDFIEVLKKKHAAK 206

Query: 76  EYKEMVIYVEACESGSIFEGIMPKDLNIYAT 104
            YKEMVIYVEACESGSIFEGIMPKDLNIY T
Sbjct: 207 GYKEMVIYVEACESGSIFEGIMPKDLNIYVT 237

[Close]

BLAST of Bhi12M000377 vs. NCBI nr
Match: KAA0039681.1 (legumain [Cucumis melo var. makuwa])

HSP 1 Score: 133.3 bits (334), Expect = 1.5e-27
Identity = 70/91 (76.92%), Postives = 71/91 (78.02%), Query Frame = 0


Query: 16  LGNITAVNRGSGKVVDSKRNDRIFVYYSD---MEALGMPTCLLSMPWTFIEVLKKKHAAK 75
           LGN TAVN GSGKVVDSK NDRIFVYYSD      LGMP         FIEVLKKKHAAK
Sbjct: 147 LGNRTAVNGGSGKVVDSKPNDRIFVYYSDHGGPGVLGMPNLPFVYAMDFIEVLKKKHAAK 206

Query: 76  EYKEMVIYVEACESGSIFEGIMPKDLNIYAT 104
            YKEMVIYVEACESGSIFEGIMP+DLNIY T
Sbjct: 207 GYKEMVIYVEACESGSIFEGIMPRDLNIYVT 237

[Close]

BLAST of Bhi12M000377 vs. NCBI nr
Match: KAG7016909.1 (hypothetical protein SDJN02_22020, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 131.7 bits (330), Expect = 4.5e-27
Identity = 69/91 (75.82%), Postives = 70/91 (76.92%), Query Frame = 0


Query: 16  LGNITAVNRGSGKVVDSKRNDRIFVYYSD---MEALGMPTCLLSMPWTFIEVLKKKHAAK 75
           LGN TAV  GSGKVVDSK NDRIFVYYSD      LGMP         FIEVLKKKHAAK
Sbjct: 152 LGNKTAVTGGSGKVVDSKPNDRIFVYYSDHGGPGVLGMPNLPFVYAMDFIEVLKKKHAAK 211

Query: 76  EYKEMVIYVEACESGSIFEGIMPKDLNIYAT 104
            YKEMVIYVEACESGSIFEG+MPKDLNIY T
Sbjct: 212 GYKEMVIYVEACESGSIFEGVMPKDLNIYVT 242

[Close]

BLAST of Bhi12M000377 vs. NCBI nr
Match: XP_022922202.1 (legumain [Cucurbita moschata] >KAG6579430.1 hypothetical protein SDJN03_23878, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 131.7 bits (330), Expect = 4.5e-27
Identity = 69/91 (75.82%), Postives = 70/91 (76.92%), Query Frame = 0


Query: 16  LGNITAVNRGSGKVVDSKRNDRIFVYYSD---MEALGMPTCLLSMPWTFIEVLKKKHAAK 75
           LGN TAV  GSGKVVDSK NDRIFVYYSD      LGMP         FIEVLKKKHAAK
Sbjct: 148 LGNKTAVTGGSGKVVDSKPNDRIFVYYSDHGGPGVLGMPNLPFVYAMDFIEVLKKKHAAK 207

Query: 76  EYKEMVIYVEACESGSIFEGIMPKDLNIYAT 104
            YKEMVIYVEACESGSIFEG+MPKDLNIY T
Sbjct: 208 GYKEMVIYVEACESGSIFEGVMPKDLNIYVT 238

[Close]

BLAST of Bhi12M000377 vs. NCBI nr
Match: XP_022969871.1 (legumain [Cucurbita maxima])

HSP 1 Score: 131.7 bits (330), Expect = 4.5e-27
Identity = 69/91 (75.82%), Postives = 70/91 (76.92%), Query Frame = 0


Query: 16  LGNITAVNRGSGKVVDSKRNDRIFVYYSD---MEALGMPTCLLSMPWTFIEVLKKKHAAK 75
           LGN TAV  GSGKVVDSK NDRIFVYYSD      LGMP         FIEVLKKKHAAK
Sbjct: 148 LGNKTAVTGGSGKVVDSKPNDRIFVYYSDHGGPGVLGMPNLPFVYAMDFIEVLKKKHAAK 207

Query: 76  EYKEMVIYVEACESGSIFEGIMPKDLNIYAT 104
            YKEMVIYVEACESGSIFEG+MPKDLNIY T
Sbjct: 208 GYKEMVIYVEACESGSIFEGVMPKDLNIYVT 238

[Close]

BLAST of Bhi12M000377 vs. ExPASy TrEMBL
Match: A0A1S3ATV0 (legumain OS=Cucumis melo OX=3656 GN=LOC103482645 PE=3 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 3.4e-28
Identity = 71/91 (78.02%), Postives = 71/91 (78.02%), Query Frame = 0


Query: 16  LGNITAVNRGSGKVVDSKRNDRIFVYYSD---MEALGMPTCLLSMPWTFIEVLKKKHAAK 75
           LGN TAVN GSGKVVDSK NDRIFVYYSD      LGMP         FIEVLKKKHAAK
Sbjct: 147 LGNRTAVNGGSGKVVDSKPNDRIFVYYSDHGGPGVLGMPNLPFVYAMDFIEVLKKKHAAK 206

Query: 76  EYKEMVIYVEACESGSIFEGIMPKDLNIYAT 104
            YKEMVIYVEACESGSIFEGIMPKDLNIY T
Sbjct: 207 GYKEMVIYVEACESGSIFEGIMPKDLNIYVT 237

[Close]

BLAST of Bhi12M000377 vs. ExPASy TrEMBL
Match: A0A5A7TDF7 (Legumain OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold558G00120 PE=3 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 7.5e-28
Identity = 70/91 (76.92%), Postives = 71/91 (78.02%), Query Frame = 0


Query: 16  LGNITAVNRGSGKVVDSKRNDRIFVYYSD---MEALGMPTCLLSMPWTFIEVLKKKHAAK 75
           LGN TAVN GSGKVVDSK NDRIFVYYSD      LGMP         FIEVLKKKHAAK
Sbjct: 147 LGNRTAVNGGSGKVVDSKPNDRIFVYYSDHGGPGVLGMPNLPFVYAMDFIEVLKKKHAAK 206

Query: 76  EYKEMVIYVEACESGSIFEGIMPKDLNIYAT 104
            YKEMVIYVEACESGSIFEGIMP+DLNIY T
Sbjct: 207 GYKEMVIYVEACESGSIFEGIMPRDLNIYVT 237

[Close]

BLAST of Bhi12M000377 vs. ExPASy TrEMBL
Match: A0A6J1HZ01 (legumain OS=Cucurbita maxima OX=3661 GN=LOC111468940 PE=3 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 2.2e-27
Identity = 69/91 (75.82%), Postives = 70/91 (76.92%), Query Frame = 0


Query: 16  LGNITAVNRGSGKVVDSKRNDRIFVYYSD---MEALGMPTCLLSMPWTFIEVLKKKHAAK 75
           LGN TAV  GSGKVVDSK NDRIFVYYSD      LGMP         FIEVLKKKHAAK
Sbjct: 148 LGNKTAVTGGSGKVVDSKPNDRIFVYYSDHGGPGVLGMPNLPFVYAMDFIEVLKKKHAAK 207

Query: 76  EYKEMVIYVEACESGSIFEGIMPKDLNIYAT 104
            YKEMVIYVEACESGSIFEG+MPKDLNIY T
Sbjct: 208 GYKEMVIYVEACESGSIFEGVMPKDLNIYVT 238

[Close]

BLAST of Bhi12M000377 vs. ExPASy TrEMBL
Match: A0A6J1E2K0 (legumain OS=Cucurbita moschata OX=3662 GN=LOC111430261 PE=3 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 2.2e-27
Identity = 69/91 (75.82%), Postives = 70/91 (76.92%), Query Frame = 0


Query: 16  LGNITAVNRGSGKVVDSKRNDRIFVYYSD---MEALGMPTCLLSMPWTFIEVLKKKHAAK 75
           LGN TAV  GSGKVVDSK NDRIFVYYSD      LGMP         FIEVLKKKHAAK
Sbjct: 148 LGNKTAVTGGSGKVVDSKPNDRIFVYYSDHGGPGVLGMPNLPFVYAMDFIEVLKKKHAAK 207

Query: 76  EYKEMVIYVEACESGSIFEGIMPKDLNIYAT 104
            YKEMVIYVEACESGSIFEG+MPKDLNIY T
Sbjct: 208 GYKEMVIYVEACESGSIFEGVMPKDLNIYVT 238

[Close]

BLAST of Bhi12M000377 vs. ExPASy TrEMBL
Match: A0A0A0KMT9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G160170 PE=3 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 2.8e-27
Identity = 69/91 (75.82%), Postives = 71/91 (78.02%), Query Frame = 0


Query: 16  LGNITAVNRGSGKVVDSKRNDRIFVYYSD---MEALGMPTCLLSMPWTFIEVLKKKHAAK 75
           LGN TAV+ GSGKVVDSK NDRIFVYYSD      LGMP         FIEVLKKKHAAK
Sbjct: 143 LGNRTAVDGGSGKVVDSKPNDRIFVYYSDHGGPGVLGMPNLPFVYAMDFIEVLKKKHAAK 202

Query: 76  EYKEMVIYVEACESGSIFEGIMPKDLNIYAT 104
            YKEMVIYVEACESGSIFEGI+PKDLNIY T
Sbjct: 203 GYKEMVIYVEACESGSIFEGILPKDLNIYVT 233

The following BLAST results are available for this feature:

BLAST of Bhi12M000377 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G62710.1	6.9e-26	67.03	beta vacuolar processing enzyme	[more]
AT2G25940.1	1.6e-22	59.34	alpha-vacuolar processing enzyme	[more]
AT4G32940.1	1.6e-22	59.34	gamma vacuolar processing enzyme	[more]
AT3G20210.1	4.6e-22	48.00	delta vacuolar processing enzyme	[more]
AT3G20210.2	4.6e-22	48.00	delta vacuolar processing enzyme	[more]

BLAST of Bhi12M000377 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P49042	2.3e-26	69.23	Vacuolar-processing enzyme OS=Ricinus communis OX=3988 PE=1 SV=1	[more]
O24326	2.0e-25	67.03	Vacuolar-processing enzyme OS=Phaseolus vulgaris OX=3885 PE=2 SV=1	[more]
P49045	4.4e-25	63.74	Vacuolar-processing enzyme OS=Glycine max OX=3847 PE=2 SV=1	[more]
Q39044	9.7e-25	67.03	Vacuolar-processing enzyme beta-isozyme OS=Arabidopsis thaliana OX=3702 GN=bVPE ...	[more]
P49046	2.2e-24	62.64	Legumain OS=Canavalia ensiformis OX=3823 PE=1 SV=1	[more]

BLAST of Bhi12M000377 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008437131.1	6.9e-28	78.02	PREDICTED: legumain [Cucumis melo]	[more]
KAA0039681.1	1.5e-27	76.92	legumain [Cucumis melo var. makuwa]	[more]
KAG7016909.1	4.5e-27	75.82	hypothetical protein SDJN02_22020, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022922202.1	4.5e-27	75.82	legumain [Cucurbita moschata] >KAG6579430.1 hypothetical protein SDJN03_23878, p...	[more]
XP_022969871.1	4.5e-27	75.82	legumain [Cucurbita maxima]	[more]

BLAST of Bhi12M000377 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3ATV0	3.4e-28	78.02	legumain OS=Cucumis melo OX=3656 GN=LOC103482645 PE=3 SV=1	[more]
A0A5A7TDF7	7.5e-28	76.92	Legumain OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold558G00120 PE=3 ...	[more]
A0A6J1HZ01	2.2e-27	75.82	legumain OS=Cucurbita maxima OX=3661 GN=LOC111468940 PE=3 SV=1	[more]
A0A6J1E2K0	2.2e-27	75.82	legumain OS=Cucurbita moschata OX=3662 GN=LOC111430261 PE=3 SV=1	[more]
A0A0A0KMT9	2.8e-27	75.82	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G160170 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001096	Peptidase C13, legumain	PRINTS	PR00776	HEMOGLOBNASE	coord: 28..47 score: 47.61 coord: 74..90 score: 69.05
IPR001096	Peptidase C13, legumain	PFAM	PF01650	Peptidase_C13	coord: 16..106 e-value: 9.8E-23 score: 81.0
IPR001096	Peptidase C13, legumain	PANTHER	PTHR12000	HEMOGLOBINASE FAMILY MEMBER	coord: 15..105
None	No IPR available	GENE3D	3.40.50.1460		coord: 11..128 e-value: 4.3E-22 score: 80.8

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Bhi12G000377	Bhi12G000377	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Bhi12M000377.exon3	Bhi12M000377.exon3	exon
Bhi12M000377.exon2	Bhi12M000377.exon2	exon
Bhi12M000377.exon1	Bhi12M000377.exon1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Bhi12M000377	cds.Bhi12M000377_3	CDS
cds.Bhi12M000377	cds.Bhi12M000377_2	CDS
cds.Bhi12M000377	cds.Bhi12M000377	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Bhi12M000377	Bhi12M000377-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
biological_process	GO:0051603	proteolysis involved in cellular protein catabolic process
biological_process	GO:0006624	vacuolar protein processing
cellular_component	GO:0110165	cellular anatomical entity
molecular_function	GO:0008233	peptidase activity
molecular_function	GO:0004197	cysteine-type endopeptidase activity