Bhi11M000534 (mRNA) Wax gourd (B227) v1

Overview


Name	Bhi11M000534
Type	mRNA
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Calcium-binding EF-hand family protein
Location	chr11: 15735147 .. 15736195 (-)
Sequence length	1049
RNA-Seq Expression	Bhi11M000534
Synteny	Bhi11M000534

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGGAAACAAGCAGCCATGAAATAAGTAAATGACGGTTATACCCTTAGGACCTTCTGAGAAGATACCCCTTGGCCTACAAAGCTTCATCATCTCATCTGTACCTGAGTTTGTCTTTTATGTTCAATTCTCTCCTTCTCGGAAAACCCCAGCAATCTTCTTCATTTTCTTCCCCTTTTTCTCTATATATCCCTTCCCATTCTTTTTCATTCACAATCCAAATCCAAATTTACTTCTCAATTTCTGTACTCTTCCATGGCCTCCAAGAACCAAATCCCCCAATCGGAATCTCGATTCGAAGATTGGCTGCCGGCGATGGCCTACAACCTCGGCGGCGAGGGGCTGATCGGAGAGCTCTGTAATGGCTTCAATCTGCTGATGGATAGAGAGAAGGGAGTGATAAATTTCGAGAGCTTGAAGAGGAATGCGGCCGTGCTAGGGCTTGGGGATCTGACGGATGAGGAGCTTAGAGGAATGTTGAGAGAAGGGGATTTCGATGGCGATGGCGCTCTTAATAAGATGGAATTTTGTGTTCTTATGTTTAGATTAAGTCCTCAGTTGATGGAAGCTTCCCGCTATTGCTTCCATGAATGTCTCCGTCGAGAATTTAGGGATTTTTCTTGATTTTTTTTTCTTCAATGAATTGGATTACTGATTTGCTTGTGTGTATTGTTCTAAATTTTTTGAGATGAAATTAGTGAAATATACTGGAAAAAATCACAAACAATTTTGACTCTCTGTTTCTCTCTCTCTCTCTCTATATGTTTTTGGTTAAGTTGTTAACTTCATCTAGTTTTAATTTTTGTTTAACTTAATTACTCTACAAAATTTTGAAACTAATTCATGCACTTTCAAGTTCAACACTTAATCTTACCTTCCTGTTGATGCAGATCATTTTGTTCCATATGACCCAATCACTAAGGATGAAAGAGAAGTCAAAACTTTGACATAGTTTATTACCATAATATTTATAAATAATTGGTCACATTAGGAGATATATGATAAGTTTGTTATTTTTTAAACCACAAAACATAAGGAATAAATGATAGCAC

mRNA sequence

CGGAAACAAGCAGCCATGAAATAAGTAAATGACGGTTATACCCTTAGGACCTTCTGAGAAGATACCCCTTGGCCTACAAAGCTTCATCATCTCATCTGTACCTGAGTTTGTCTTTTATGTTCAATTCTCTCCTTCTCGGAAAACCCCAGCAATCTTCTTCATTTTCTTCCCCTTTTTCTCTATATATCCCTTCCCATTCTTTTTCATTCACAATCCAAATCCAAATTTACTTCTCAATTTCTGTACTCTTCCATGGCCTCCAAGAACCAAATCCCCCAATCGGAATCTCGATTCGAAGATTGGCTGCCGGCGATGGCCTACAACCTCGGCGGCGAGGGGCTGATCGGAGAGCTCTGTAATGGCTTCAATCTGCTGATGGATAGAGAGAAGGGAGTGATAAATTTCGAGAGCTTGAAGAGGAATGCGGCCGTGCTAGGGCTTGGGGATCTGACGGATGAGGAGCTTAGAGGAATGTTGAGAGAAGGGGATTTCGATGGCGATGGCGCTCTTAATAAGATGGAATTTTGTGTTCTTATGTTTAGATTAAGTCCTCAGTTGATGGAAGCTTCCCGCTATTGCTTCCATGAATGTCTCCGTCGAGAATTTAGGGATTTTTCTTGATTTTTTTTTCTTCAATGAATTGGATTACTGATTTGCTTGTGTGTATTGTTCTAAATTTTTTGAGATGAAATTAGTGAAATATACTGGAAAAAATCACAAACAATTTTGACTCTCTGTTTCTCTCTCTCTCTCTCTATATGTTTTTGGTTAAGTTGTTAACTTCATCTAGTTTTAATTTTTGTTTAACTTAATTACTCTACAAAATTTTGAAACTAATTCATGCACTTTCAAGTTCAACACTTAATCTTACCTTCCTGTTGATGCAGATCATTTTGTTCCATATGACCCAATCACTAAGGATGAAAGAGAAGTCAAAACTTTGACATAGTTTATTACCATAATATTTATAAATAATTGGTCACATTAGGAGATATATGATAAGTTTGTTATTTTTTAAACCACAAAACATAAGGAATAAATGATAGCAC

Coding sequence (CDS)

ATGGCCTCCAAGAACCAAATCCCCCAATCGGAATCTCGATTCGAAGATTGGCTGCCGGCGATGGCCTACAACCTCGGCGGCGAGGGGCTGATCGGAGAGCTCTGTAATGGCTTCAATCTGCTGATGGATAGAGAGAAGGGAGTGATAAATTTCGAGAGCTTGAAGAGGAATGCGGCCGTGCTAGGGCTTGGGGATCTGACGGATGAGGAGCTTAGAGGAATGTTGAGAGAAGGGGATTTCGATGGCGATGGCGCTCTTAATAAGATGGAATTTTGTGTTCTTATGTTTAGATTAAGTCCTCAGTTGATGGAAGCTTCCCGCTATTGCTTCCATGAATGTCTCCGTCGAGAATTTAGGGATTTTTCTTGA

Protein sequence

MASKNQIPQSESRFEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAVLGLGDLTDEELRGMLREGDFDGDGALNKMEFCVLMFRLSPQLMEASRYCFHECLRREFRDFS

Homology

[Close]

BLAST of Bhi11M000534 vs. TAIR 10
Match: AT4G27280.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 163.7 bits (413), Expect = 9.3e-41
Identity = 82/109 (75.23%), Postives = 91/109 (83.49%), Query Frame = 0


Query: 11  ESRFEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRN-AAVLGLGDLTDE 70
           +  F D+LP MA NLGGEGLIGELCNGF LLMDREKGVI FESL+RN AAVLGLGDLTDE
Sbjct: 16  QPNFHDFLPTMAGNLGGEGLIGELCNGFELLMDREKGVITFESLRRNAAAVLGLGDLTDE 75

Query: 71  ELRGMLREGDFDGDGALNKMEFCVLMFRLSPQLMEASRYCFHECLRREF 119
           ++R M++EGDFD DGALN+MEFCVLMFRLSP LMEASR    E +  EF
Sbjct: 76  DVRCMIKEGDFDCDGALNQMEFCVLMFRLSPDLMEASRCLVTEVIEEEF 124

[Close]

BLAST of Bhi11M000534 vs. TAIR 10
Match: AT5G54490.1 (pinoid-binding protein 1 )

HSP 1 Score: 152.1 bits (383), Expect = 2.8e-37
Identity = 77/116 (66.38%), Postives = 92/116 (79.31%), Query Frame = 0


Query: 4   KNQIPQSESRFEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAA-VLG 63
           +NQ PQ    F+D+ P MA  LGGEGLI E+C GF LLMD++KGVI FESL+RNA+ VLG
Sbjct: 13  ENQEPQ----FQDFFPTMAGKLGGEGLIEEICKGFELLMDKDKGVITFESLRRNASTVLG 72

Query: 64  LGDLTDEELRGMLREGDFDGDGALNKMEFCVLMFRLSPQLMEASRYCFHECLRREF 119
           LGDLTD+++R M+ EGDFD DGALN+MEFCVLMFRLSP+LMEASR    E +  EF
Sbjct: 73  LGDLTDDDVRYMINEGDFDRDGALNQMEFCVLMFRLSPELMEASRCVVTEVIEEEF 124

[Close]

BLAST of Bhi11M000534 vs. TAIR 10
Match: AT2G46600.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 115.5 bits (288), Expect = 2.9e-26
Identity = 53/107 (49.53%), Postives = 76/107 (71.03%), Query Frame = 0


Query: 11  ESRFEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAVLGLGDLTDEE 70
           E+++ED LP MA  +  E  + ELC GF+LL D E+ +I  ESL+RN+ +LG+  ++ E+
Sbjct: 19  ETKYEDMLPVMAEKMDVEEFVSELCKGFSLLADPERHLITAESLRRNSGILGIEGMSKED 78

Query: 71  LRGMLREGDFDGDGALNKMEFCVLMFRLSPQLMEASRYCFHECLRRE 118
            +GM+REGD DGDGALN+ EFCVLM RLSP++ME +     + L +E
Sbjct: 79  AQGMVREGDLDGDGALNQTEFCVLMVRLSPEMMEDAETWLEKALTQE 125

[Close]

BLAST of Bhi11M000534 vs. TAIR 10
Match: AT4G14640.1 (calmodulin 8 )

HSP 1 Score: 46.6 bits (109), Expect = 1.7e-05
Identity = 29/86 (33.72%), Postives = 43/86 (50.00%), Query Frame = 0


Query: 14  FEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAVLGLGD-LTDEELR 73
           F ++L  MA  L       EL   F +    + G I+   L     ++ LG+ LTDEE+ 
Sbjct: 67  FAEFLNLMAKKLQESDAEEELKEAFKVFDKDQNGYISASELSH--VMINLGEKLTDEEVE 126

Query: 74  GMLREGDFDGDGALNKMEFCVLMFRL 99
            M++E D DGDG +N  EF  +M  +
Sbjct: 127 QMIKEADLDGDGQVNYDEFVKMMINI 150

[Close]

BLAST of Bhi11M000534 vs. TAIR 10
Match: AT1G66410.1 (calmodulin 4 )

HSP 1 Score: 45.8 bits (107), Expect = 2.8e-05
Identity = 29/82 (35.37%), Postives = 40/82 (48.78%), Query Frame = 0


Query: 14  FEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAVLGLGDLTDEELRG 73
           F ++L  MA  +       EL   F +    + G I+   L+     LG   LTDEE+  
Sbjct: 66  FPEFLNLMAKKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLG-EKLTDEEVEE 125

Query: 74  MLREGDFDGDGALNKMEFCVLM 96
           M+RE D DGDG +N  EF  +M
Sbjct: 126 MIREADVDGDGQINYEEFVKIM 146

[Close]

BLAST of Bhi11M000534 vs. ExPASy Swiss-Prot
Match: O81831 (Calcium-binding protein KRP1 OS=Arabidopsis thaliana OX=3702 GN=KRP1 PE=2 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 1.3e-39
Identity = 82/109 (75.23%), Postives = 91/109 (83.49%), Query Frame = 0


Query: 11  ESRFEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRN-AAVLGLGDLTDE 70
           +  F D+LP MA NLGGEGLIGELCNGF LLMDREKGVI FESL+RN AAVLGLGDLTDE
Sbjct: 16  QPNFHDFLPTMAGNLGGEGLIGELCNGFELLMDREKGVITFESLRRNAAAVLGLGDLTDE 75

Query: 71  ELRGMLREGDFDGDGALNKMEFCVLMFRLSPQLMEASRYCFHECLRREF 119
           ++R M++EGDFD DGALN+MEFCVLMFRLSP LMEASR    E +  EF
Sbjct: 76  DVRCMIKEGDFDCDGALNQMEFCVLMFRLSPDLMEASRCLVTEVIEEEF 124

[Close]

BLAST of Bhi11M000534 vs. ExPASy Swiss-Prot
Match: Q9LSQ6 (Calcium-binding protein PBP1 OS=Arabidopsis thaliana OX=3702 GN=PBP1 PE=1 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 4.0e-36
Identity = 77/116 (66.38%), Postives = 92/116 (79.31%), Query Frame = 0


Query: 4   KNQIPQSESRFEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAA-VLG 63
           +NQ PQ    F+D+ P MA  LGGEGLI E+C GF LLMD++KGVI FESL+RNA+ VLG
Sbjct: 13  ENQEPQ----FQDFFPTMAGKLGGEGLIEEICKGFELLMDKDKGVITFESLRRNASTVLG 72

Query: 64  LGDLTDEELRGMLREGDFDGDGALNKMEFCVLMFRLSPQLMEASRYCFHECLRREF 119
           LGDLTD+++R M+ EGDFD DGALN+MEFCVLMFRLSP+LMEASR    E +  EF
Sbjct: 73  LGDLTDDDVRYMINEGDFDRDGALNQMEFCVLMFRLSPELMEASRCVVTEVIEEEF 124

[Close]

BLAST of Bhi11M000534 vs. ExPASy Swiss-Prot
Match: Q9ZPX9 (Calcium-binding protein KIC OS=Arabidopsis thaliana OX=3702 GN=KIC PE=1 SV=2)

HSP 1 Score: 115.5 bits (288), Expect = 4.1e-25
Identity = 53/107 (49.53%), Postives = 76/107 (71.03%), Query Frame = 0


Query: 11  ESRFEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAVLGLGDLTDEE 70
           E+++ED LP MA  +  E  + ELC GF+LL D E+ +I  ESL+RN+ +LG+  ++ E+
Sbjct: 19  ETKYEDMLPVMAEKMDVEEFVSELCKGFSLLADPERHLITAESLRRNSGILGIEGMSKED 78

Query: 71  LRGMLREGDFDGDGALNKMEFCVLMFRLSPQLMEASRYCFHECLRRE 118
            +GM+REGD DGDGALN+ EFCVLM RLSP++ME +     + L +E
Sbjct: 79  AQGMVREGDLDGDGALNQTEFCVLMVRLSPEMMEDAETWLEKALTQE 125

[Close]

BLAST of Bhi11M000534 vs. ExPASy Swiss-Prot
Match: Q9R1K9 (Centrin-2 OS=Mus musculus OX=10090 GN=Cetn2 PE=1 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 1.8e-09
Identity = 36/86 (41.86%), Postives = 50/86 (58.14%), Query Frame = 0


Query: 14  FEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAVLGLGDLTDEELRG 73
           F D+L  M   +  +    E+   F L  D E G I+F++LKR A  LG  +LTDEEL+ 
Sbjct: 86  FSDFLTVMTQKMSEKDTKEEILKAFKLFDDDETGKISFKNLKRVAKELG-ENLTDEELQE 145

Query: 74  MLREGDFDGDGALNKMEFCVLMFRLS 100
           M+ E D DGDG +N+ EF  +M + S
Sbjct: 146 MIDEADRDGDGEVNEQEFLRIMKKTS 170

[Close]

BLAST of Bhi11M000534 vs. ExPASy Swiss-Prot
Match: Q06827 (Caltractin OS=Scherffelia dubia OX=3190 PE=1 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 3.2e-09
Identity = 36/86 (41.86%), Postives = 51/86 (59.30%), Query Frame = 0


Query: 14  FEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAVLGLGDLTDEELRG 73
           FE++L  M   +G      E+   F L  D E G I+F++LKR A  LG  ++TDEEL+ 
Sbjct: 82  FEEFLQMMTAKMGERDSREEIMKAFRLFDDDETGKISFKNLKRVAKELG-ENMTDEELQE 141

Query: 74  MLREGDFDGDGALNKMEFCVLMFRLS 100
           M+ E D DGDG +N+ EF  +M + S
Sbjct: 142 MIDEADRDGDGEVNEEEFFRIMKKTS 166

[Close]

BLAST of Bhi11M000534 vs. NCBI nr
Match: XP_038905287.1 (calcium-binding protein KRP1-like [Benincasa hispida])

HSP 1 Score: 254.2 bits (648), Expect = 5.6e-64
Identity = 122/122 (100.00%), Postives = 122/122 (100.00%), Query Frame = 0


Query: 1   MASKNQIPQSESRFEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAV 60
           MASKNQIPQSESRFEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAV
Sbjct: 1   MASKNQIPQSESRFEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAV 60

Query: 61  LGLGDLTDEELRGMLREGDFDGDGALNKMEFCVLMFRLSPQLMEASRYCFHECLRREFRD 120
           LGLGDLTDEELRGMLREGDFDGDGALNKMEFCVLMFRLSPQLMEASRYCFHECLRREFRD
Sbjct: 61  LGLGDLTDEELRGMLREGDFDGDGALNKMEFCVLMFRLSPQLMEASRYCFHECLRREFRD 120

Query: 121 FS 123
           FS
Sbjct: 121 FS 122

[Close]

BLAST of Bhi11M000534 vs. NCBI nr
Match: XP_004152909.1 (calcium-binding protein KRP1 [Cucumis sativus] >KGN56165.1 hypothetical protein Csa_010073 [Cucumis sativus])

HSP 1 Score: 226.9 bits (577), Expect = 9.6e-56
Identity = 108/120 (90.00%), Postives = 113/120 (94.17%), Query Frame = 0


Query: 1   MASKNQIPQSESRFEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAV 60
           M  KNQIPQSES F+DWLP MA NLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAA 
Sbjct: 1   MTPKNQIPQSESGFQDWLPVMAGNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAA 60

Query: 61  LGLGDLTDEELRGMLREGDFDGDGALNKMEFCVLMFRLSPQLMEASRYCFHECLRREFRD 120
           LGLGDL+D+ELRGMLREGDFDGDGALN+MEFCVLMFRLSP+LMEASRYCFHE LRREFRD
Sbjct: 61  LGLGDLSDDELRGMLREGDFDGDGALNQMEFCVLMFRLSPELMEASRYCFHESLRREFRD 120

[Close]

BLAST of Bhi11M000534 vs. NCBI nr
Match: XP_008463452.1 (PREDICTED: calcium-binding protein PBP1-like [Cucumis melo] >KAA0025444.1 calcium-binding protein PBP1-like [Cucumis melo var. makuwa] >TYK04738.1 calcium-binding protein PBP1-like [Cucumis melo var. makuwa])

HSP 1 Score: 218.4 bits (555), Expect = 3.4e-53
Identity = 104/120 (86.67%), Postives = 110/120 (91.67%), Query Frame = 0


Query: 1   MASKNQIPQSESRFEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAV 60
           M S N IPQSES F+DWLP MA  LGGEGLIGELCNGFNLLMDREKGVINFESLKRNAA 
Sbjct: 1   MTSNNHIPQSESGFQDWLPVMAGKLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAA 60

Query: 61  LGLGDLTDEELRGMLREGDFDGDGALNKMEFCVLMFRLSPQLMEASRYCFHECLRREFRD 120
           LGLGDL+D+ELRGMLREGD +GDGALN+MEFCVLMFRLSP+LMEASRYCFHE LRREFRD
Sbjct: 61  LGLGDLSDDELRGMLREGDLNGDGALNQMEFCVLMFRLSPELMEASRYCFHESLRREFRD 120

[Close]

BLAST of Bhi11M000534 vs. NCBI nr
Match: XP_022985284.1 (calcium-binding protein PBP1-like [Cucurbita maxima])

HSP 1 Score: 213.0 bits (541), Expect = 1.4e-51
Identity = 103/122 (84.43%), Postives = 109/122 (89.34%), Query Frame = 0


Query: 1   MASKNQIPQSESRFEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAV 60
           MA  NQIPQSES FEDWLP MA  LGGEGLIGELCNGFNLLMD EKGVIN ESLKRNAA 
Sbjct: 1   MAGNNQIPQSESGFEDWLPEMACKLGGEGLIGELCNGFNLLMDPEKGVINLESLKRNAAA 60

Query: 61  LGLGDLTDEELRGMLREGDFDGDGALNKMEFCVLMFRLSPQLMEASRYCFHECLRREFRD 120
           LGLGD +DEELRGMLREGDFDGDGALN+MEFCVLMFRLSP+LM+ASRYCF + LRREF +
Sbjct: 61  LGLGDHSDEELRGMLREGDFDGDGALNQMEFCVLMFRLSPELMDASRYCFQQSLRREFPN 120

Query: 121 FS 123
           FS
Sbjct: 121 FS 122

[Close]

BLAST of Bhi11M000534 vs. NCBI nr
Match: XP_022144078.1 (calcium-binding protein PBP1-like [Momordica charantia])

HSP 1 Score: 211.1 bits (536), Expect = 5.5e-51
Identity = 102/118 (86.44%), Postives = 108/118 (91.53%), Query Frame = 0


Query: 1   MASKNQIPQSESRFEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAV 60
           MA+KNQ PQS+S FEDWLPAMA+ LGGEGLIGELCNGFNLLMDREKGVINFESLKRNAA 
Sbjct: 1   MAAKNQSPQSQSGFEDWLPAMAWKLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAA 60

Query: 61  LGLGDLTDEELRGMLREGDFDGDGALNKMEFCVLMFRLSPQLMEASRYCFHECLRREF 119
           LGLGDL+DEELRGML EGDFDGDGALN+MEFCVLMFRLSP LM+ASRY   E LRREF
Sbjct: 61  LGLGDLSDEELRGMLTEGDFDGDGALNQMEFCVLMFRLSPDLMDASRYWLQESLRREF 118

[Close]

BLAST of Bhi11M000534 vs. ExPASy TrEMBL
Match: A0A0A0L2H0 (EF-hand domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G081900 PE=4 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 4.7e-56
Identity = 108/120 (90.00%), Postives = 113/120 (94.17%), Query Frame = 0


Query: 1   MASKNQIPQSESRFEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAV 60
           M  KNQIPQSES F+DWLP MA NLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAA 
Sbjct: 1   MTPKNQIPQSESGFQDWLPVMAGNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAA 60

Query: 61  LGLGDLTDEELRGMLREGDFDGDGALNKMEFCVLMFRLSPQLMEASRYCFHECLRREFRD 120
           LGLGDL+D+ELRGMLREGDFDGDGALN+MEFCVLMFRLSP+LMEASRYCFHE LRREFRD
Sbjct: 61  LGLGDLSDDELRGMLREGDFDGDGALNQMEFCVLMFRLSPELMEASRYCFHESLRREFRD 120

[Close]

BLAST of Bhi11M000534 vs. ExPASy TrEMBL
Match: A0A5A7SII1 (Calcium-binding protein PBP1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold68G00340 PE=4 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 1.7e-53
Identity = 104/120 (86.67%), Postives = 110/120 (91.67%), Query Frame = 0


Query: 1   MASKNQIPQSESRFEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAV 60
           M S N IPQSES F+DWLP MA  LGGEGLIGELCNGFNLLMDREKGVINFESLKRNAA 
Sbjct: 1   MTSNNHIPQSESGFQDWLPVMAGKLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAA 60

Query: 61  LGLGDLTDEELRGMLREGDFDGDGALNKMEFCVLMFRLSPQLMEASRYCFHECLRREFRD 120
           LGLGDL+D+ELRGMLREGD +GDGALN+MEFCVLMFRLSP+LMEASRYCFHE LRREFRD
Sbjct: 61  LGLGDLSDDELRGMLREGDLNGDGALNQMEFCVLMFRLSPELMEASRYCFHESLRREFRD 120

[Close]

BLAST of Bhi11M000534 vs. ExPASy TrEMBL
Match: A0A1S3CKV7 (calcium-binding protein PBP1-like OS=Cucumis melo OX=3656 GN=LOC103501619 PE=4 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 1.7e-53
Identity = 104/120 (86.67%), Postives = 110/120 (91.67%), Query Frame = 0


Query: 1   MASKNQIPQSESRFEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAV 60
           M S N IPQSES F+DWLP MA  LGGEGLIGELCNGFNLLMDREKGVINFESLKRNAA 
Sbjct: 1   MTSNNHIPQSESGFQDWLPVMAGKLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAA 60

Query: 61  LGLGDLTDEELRGMLREGDFDGDGALNKMEFCVLMFRLSPQLMEASRYCFHECLRREFRD 120
           LGLGDL+D+ELRGMLREGD +GDGALN+MEFCVLMFRLSP+LMEASRYCFHE LRREFRD
Sbjct: 61  LGLGDLSDDELRGMLREGDLNGDGALNQMEFCVLMFRLSPELMEASRYCFHESLRREFRD 120

[Close]

BLAST of Bhi11M000534 vs. ExPASy TrEMBL
Match: A0A6J1JCW0 (calcium-binding protein PBP1-like OS=Cucurbita maxima OX=3661 GN=LOC111483323 PE=4 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 7.0e-52
Identity = 103/122 (84.43%), Postives = 109/122 (89.34%), Query Frame = 0


Query: 1   MASKNQIPQSESRFEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAV 60
           MA  NQIPQSES FEDWLP MA  LGGEGLIGELCNGFNLLMD EKGVIN ESLKRNAA 
Sbjct: 1   MAGNNQIPQSESGFEDWLPEMACKLGGEGLIGELCNGFNLLMDPEKGVINLESLKRNAAA 60

Query: 61  LGLGDLTDEELRGMLREGDFDGDGALNKMEFCVLMFRLSPQLMEASRYCFHECLRREFRD 120
           LGLGD +DEELRGMLREGDFDGDGALN+MEFCVLMFRLSP+LM+ASRYCF + LRREF +
Sbjct: 61  LGLGDHSDEELRGMLREGDFDGDGALNQMEFCVLMFRLSPELMDASRYCFQQSLRREFPN 120

Query: 121 FS 123
           FS
Sbjct: 121 FS 122

[Close]

BLAST of Bhi11M000534 vs. ExPASy TrEMBL
Match: A0A6J1CSD5 (calcium-binding protein PBP1-like OS=Momordica charantia OX=3673 GN=LOC111013856 PE=4 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 2.6e-51
Identity = 102/118 (86.44%), Postives = 108/118 (91.53%), Query Frame = 0


Query: 1   MASKNQIPQSESRFEDWLPAMAYNLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAV 60
           MA+KNQ PQS+S FEDWLPAMA+ LGGEGLIGELCNGFNLLMDREKGVINFESLKRNAA 
Sbjct: 1   MAAKNQSPQSQSGFEDWLPAMAWKLGGEGLIGELCNGFNLLMDREKGVINFESLKRNAAA 60

Query: 61  LGLGDLTDEELRGMLREGDFDGDGALNKMEFCVLMFRLSPQLMEASRYCFHECLRREF 119
           LGLGDL+DEELRGML EGDFDGDGALN+MEFCVLMFRLSP LM+ASRY   E LRREF
Sbjct: 61  LGLGDLSDEELRGMLTEGDFDGDGALNQMEFCVLMFRLSPDLMDASRYWLQESLRREF 118

The following BLAST results are available for this feature:

BLAST of Bhi11M000534 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G27280.1	9.3e-41	75.23	Calcium-binding EF-hand family protein	[more]
AT5G54490.1	2.8e-37	66.38	pinoid-binding protein 1	[more]
AT2G46600.1	2.9e-26	49.53	Calcium-binding EF-hand family protein	[more]
AT4G14640.1	1.7e-05	33.72	calmodulin 8	[more]
AT1G66410.1	2.8e-05	35.37	calmodulin 4	[more]

BLAST of Bhi11M000534 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81831	1.3e-39	75.23	Calcium-binding protein KRP1 OS=Arabidopsis thaliana OX=3702 GN=KRP1 PE=2 SV=1	[more]
Q9LSQ6	4.0e-36	66.38	Calcium-binding protein PBP1 OS=Arabidopsis thaliana OX=3702 GN=PBP1 PE=1 SV=1	[more]
Q9ZPX9	4.1e-25	49.53	Calcium-binding protein KIC OS=Arabidopsis thaliana OX=3702 GN=KIC PE=1 SV=2	[more]
Q9R1K9	1.8e-09	41.86	Centrin-2 OS=Mus musculus OX=10090 GN=Cetn2 PE=1 SV=1	[more]
Q06827	3.2e-09	41.86	Caltractin OS=Scherffelia dubia OX=3190 PE=1 SV=1	[more]

BLAST of Bhi11M000534 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038905287.1	5.6e-64	100.00	calcium-binding protein KRP1-like [Benincasa hispida]	[more]
XP_004152909.1	9.6e-56	90.00	calcium-binding protein KRP1 [Cucumis sativus] >KGN56165.1 hypothetical protein ...	[more]
XP_008463452.1	3.4e-53	86.67	PREDICTED: calcium-binding protein PBP1-like [Cucumis melo] >KAA0025444.1 calciu...	[more]
XP_022985284.1	1.4e-51	84.43	calcium-binding protein PBP1-like [Cucurbita maxima]	[more]
XP_022144078.1	5.5e-51	86.44	calcium-binding protein PBP1-like [Momordica charantia]	[more]

BLAST of Bhi11M000534 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L2H0	4.7e-56	90.00	EF-hand domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G081900 PE=...	[more]
A0A5A7SII1	1.7e-53	86.67	Calcium-binding protein PBP1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A1S3CKV7	1.7e-53	86.67	calcium-binding protein PBP1-like OS=Cucumis melo OX=3656 GN=LOC103501619 PE=4 S...	[more]
A0A6J1JCW0	7.0e-52	84.43	calcium-binding protein PBP1-like OS=Cucurbita maxima OX=3661 GN=LOC111483323 PE...	[more]
A0A6J1CSD5	2.6e-51	86.44	calcium-binding protein PBP1-like OS=Momordica charantia OX=3673 GN=LOC111013856...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 70..98 e-value: 0.0072 score: 25.5
IPR002048	EF-hand domain	PFAM	PF13833	EF-hand_8	coord: 45..97 e-value: 1.1E-17 score: 63.6
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 66..101 score: 10.384886
None	No IPR available	GENE3D	1.10.238.10		coord: 11..121 e-value: 6.1E-19 score: 70.0
None	No IPR available	PANTHER	PTHR47319:SF6	CALCIUM-BINDING PROTEIN KRP1	coord: 9..121
IPR044205	Calcium-binding protein KIC/PBP1/KRP1	PANTHER	PTHR47319	CALCIUM-BINDING PROTEIN KIC	coord: 9..121
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 79..91
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 14..104

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Bhi11G000534	Bhi11G000534	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Bhi11M000534.exon1	Bhi11M000534.exon1	exon

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Bhi11M000534.utr3p1	Bhi11M000534.utr3p1	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Bhi11M000534	cds.Bhi11M000534	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Bhi11M000534.utr5p1	Bhi11M000534.utr5p1	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Bhi11M000534	Bhi11M000534-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005509	calcium ion binding