Bhi08M000017 (mRNA) Wax gourd (B227) v1

Overview
NameBhi08M000017
TypemRNA
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionNAD(P)-binding Rossmann-fold superfamily protein
Locationchr8: 503917 .. 505426 (+)
Sequence length1404
RNA-Seq ExpressionBhi08M000017
SyntenyBhi08M000017
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCAAATTGTTGTTACTTTTCTTTTAAATATTATAATTTTCTTGTGTATGTGATGAGAGTAACACACATACAAAAATAGATCTTGAAATGCATTCAAGCAATACTCTTTTCAATTTGGTAAAATAGACATAGAATTAGAAGAGCAATATATGGGTGCAGCCTAAGCTAGGCCCAACTCCATTGCATCTATTTAATGACAAAAAGAAAACAATATTAAAAAGATATATTTTTAAATTTTGTTTACGTGACAGTTTATGATTAGAAATTTAGCTGATGGAGATGTACTATAATAACTAATATGTTTATGAAAGATGGGGAAAAAGGGAGAGAGAGATTTGGTGTGAGCAGCCATGAGTTTCCAATTGCTTCCTGCCGTTGCAAGAAGGTACTCATTTATTTTACTCTTTAACTTTACCAAAACACTTATATTATATCATTTTCAACGATTTCTTCAAACCCAAAATATATTTGATTGTTGTTTGATATATTAGACTTGAAGGCAAAGTAGCCGTAATCACTGGTGGGGCTAGAGGAATTGGTGAGCACACAGCAAGGCTCTTCCTCAAGCATGGAGCCAAGGTAGTTATTGCAGACATCGAAGATGATTTAGGACACGCTCTTTGCAACGATTATCTTGGCTCGTCATCTTCTTCCTTCGTCCATTGCGACGTCACAAAAGAGAAAGATGTCGAAAACGCCATCGACACGGCGGTTTCCAAGTATGGAAAGTTAGACATCATGTTCAACAACGCCGGAGTTTTTGATGAAGCTTCAAACTTTGACATTCTCAAAGACGATCCATTAACCTTTCAAAAAGTGGTGAACGTGAACCTTGTGGGAGCCTTTCTTGGGACGAAACACGCAGCACGGGTGATGAAACCGTGTGGTCGAGGGAGCATCGTGACGACCGCAAGTATATGCTCGGTGATCGGAGGGATTGGGACGCATGCTTATACGAGCTCGAAGCATGGGGTTTTGGGATTGATGAGGAATACAGCTGTGGATTTGGGAAGATATGGGATTAGGGTTAATTGTGTGTCACCTAATGTAGTGCCAACTGAAATGGGAAGGAAGTTGTTTAAGTTGAAAGAAGGTGATGAGTTTCCAAGTTTTTATTGGAACTTGAAAGGTGGTGATATTTTGAGGGAAGAAGATGTTGGTGAAGCTGTGGTGTATTTGGGGAGTGATGAGTCTAAGTGTGTGAGTGGACTCAACTTGATTGTTGATGGAGGATTTACTGTTGTCAACCAAGCTCTTTGCTCCTTTCAACCATCTCATTGATTAATCATGTTTCAATCAATTACATAAGTTAAAAAGAAGAATGCTTTTTATTATCATTATTACTTTAATATCTACGCTGTATTATTTTTCATATGACGAATTCTTCTTTCAACTTTTTGTTTAAATGGTTTTAAGTTTTATCAGATGAGTACGACTTCTAATATACGAAAGTATTATTATAATTATTTTTTGAATTTAAATAATAGTAGAGATCTGTATATTTGTGTTTA

mRNA sequence

GCAAATTGTTGTTACTTTTCTTTTAAATATTATAATTTTCTTGTGTATGTGATGAGAGTAACACACATACAAAAATAGATCTTGAAATGCATTCAAGCAATACTCTTTTCAATTTGGTAAAATAGACATAGAATTAGAAGAGCAATATATGGGTGCAGCCTAAGCTAGGCCCAACTCCATTGCATCTATTTAATGACAAAAAGAAAACAATATTAAAAAGATATATTTTTAAATTTTGTTTACGTGACAGTTTATGATTAGAAATTTAGCTGATGGAGATGTACTATAATAACTAATATGTTTATGAAAGATGGGGAAAAAGGGAGAGAGAGATTTGGTGTGAGCAGCCATGAGTTTCCAATTGCTTCCTGCCGTTGCAAGAAGACTTGAAGGCAAAGTAGCCGTAATCACTGGTGGGGCTAGAGGAATTGGTGAGCACACAGCAAGGCTCTTCCTCAAGCATGGAGCCAAGGTAGTTATTGCAGACATCGAAGATGATTTAGGACACGCTCTTTGCAACGATTATCTTGGCTCGTCATCTTCTTCCTTCGTCCATTGCGACGTCACAAAAGAGAAAGATGTCGAAAACGCCATCGACACGGCGGTTTCCAAGTATGGAAAGTTAGACATCATGTTCAACAACGCCGGAGTTTTTGATGAAGCTTCAAACTTTGACATTCTCAAAGACGATCCATTAACCTTTCAAAAAGTGGTGAACGTGAACCTTGTGGGAGCCTTTCTTGGGACGAAACACGCAGCACGGGTGATGAAACCGTGTGGTCGAGGGAGCATCGTGACGACCGCAAGTATATGCTCGGTGATCGGAGGGATTGGGACGCATGCTTATACGAGCTCGAAGCATGGGGTTTTGGGATTGATGAGGAATACAGCTGTGGATTTGGGAAGATATGGGATTAGGGTTAATTGTGTGTCACCTAATGTAGTGCCAACTGAAATGGGAAGGAAGTTGTTTAAGTTGAAAGAAGGTGATGAGTTTCCAAGTTTTTATTGGAACTTGAAAGGTGGTGATATTTTGAGGGAAGAAGATGTTGGTGAAGCTGTGGTGTATTTGGGGAGTGATGAGTCTAAGTGTGTGAGTGGACTCAACTTGATTGTTGATGGAGGATTTACTGTTGTCAACCAAGCTCTTTGCTCCTTTCAACCATCTCATTGATTAATCATGTTTCAATCAATTACATAAGTTAAAAAGAAGAATGCTTTTTATTATCATTATTACTTTAATATCTACGCTGTATTATTTTTCATATGACGAATTCTTCTTTCAACTTTTTGTTTAAATGGTTTTAAGTTTTATCAGATGAGTACGACTTCTAATATACGAAAGTATTATTATAATTATTTTTTGAATTTAAATAATAGTAGAGATCTGTATATTTGTGTTTA

Coding sequence (CDS)

ATGAGTTTCCAATTGCTTCCTGCCGTTGCAAGAAGACTTGAAGGCAAAGTAGCCGTAATCACTGGTGGGGCTAGAGGAATTGGTGAGCACACAGCAAGGCTCTTCCTCAAGCATGGAGCCAAGGTAGTTATTGCAGACATCGAAGATGATTTAGGACACGCTCTTTGCAACGATTATCTTGGCTCGTCATCTTCTTCCTTCGTCCATTGCGACGTCACAAAAGAGAAAGATGTCGAAAACGCCATCGACACGGCGGTTTCCAAGTATGGAAAGTTAGACATCATGTTCAACAACGCCGGAGTTTTTGATGAAGCTTCAAACTTTGACATTCTCAAAGACGATCCATTAACCTTTCAAAAAGTGGTGAACGTGAACCTTGTGGGAGCCTTTCTTGGGACGAAACACGCAGCACGGGTGATGAAACCGTGTGGTCGAGGGAGCATCGTGACGACCGCAAGTATATGCTCGGTGATCGGAGGGATTGGGACGCATGCTTATACGAGCTCGAAGCATGGGGTTTTGGGATTGATGAGGAATACAGCTGTGGATTTGGGAAGATATGGGATTAGGGTTAATTGTGTGTCACCTAATGTAGTGCCAACTGAAATGGGAAGGAAGTTGTTTAAGTTGAAAGAAGGTGATGAGTTTCCAAGTTTTTATTGGAACTTGAAAGGTGGTGATATTTTGAGGGAAGAAGATGTTGGTGAAGCTGTGGTGTATTTGGGGAGTGATGAGTCTAAGTGTGTGAGTGGACTCAACTTGATTGTTGATGGAGGATTTACTGTTGTCAACCAAGCTCTTTGCTCCTTTCAACCATCTCATTGA

Protein sequence

MSFQLLPAVARRLEGKVAVITGGARGIGEHTARLFLKHGAKVVIADIEDDLGHALCNDYLGSSSSSFVHCDVTKEKDVENAIDTAVSKYGKLDIMFNNAGVFDEASNFDILKDDPLTFQKVVNVNLVGAFLGTKHAARVMKPCGRGSIVTTASICSVIGGIGTHAYTSSKHGVLGLMRNTAVDLGRYGIRVNCVSPNVVPTEMGRKLFKLKEGDEFPSFYWNLKGGDILREEDVGEAVVYLGSDESKCVSGLNLIVDGGFTVVNQALCSFQPSH
Homology
BLAST of Bhi08M000017 vs. TAIR 10
Match: AT3G51680.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 236.5 bits (602), Expect = 2.5e-62
Identity = 129/273 (47.25%), Postives = 175/273 (64.10%), Query Frame = 0

Query: 11  RRLEGKVAVITGGARGIGEHTARLFLKHGAKVVIADIEDDLGHALCNDYLGSSSS---SF 70
           +RLEGKVA+ITGGA GIG+ T  LF +HGA VVIAD+++  G +L        +S   +F
Sbjct: 30  KRLEGKVAIITGGAHGIGKATVMLFARHGATVVIADVDNVAGSSLAKSLSSHKTSPMVAF 89

Query: 71  VHCDVTKEKDVENAIDTAVSKYGKLDIMFNNAGVF-DEASNFDILKDDPLTFQKVVNVNL 130
           + CDV+ E DVEN ++  V++YG+LDI+FNNAGV  D+  +  IL  D   F  V+ VN+
Sbjct: 90  ISCDVSVEADVENLVNVTVARYGRLDILFNNAGVLGDQKKHKSILDFDADEFDHVMRVNV 149

Query: 131 VGAFLGTKHAARVMKPCG-RGSIVTTASICSVIGGIGTHAYTSSKHGVLGLMRNTAVDLG 190
            G  LG KH AR M   G +G I++TAS+  V+GG+G HAYT+SKH ++GL +N A +LG
Sbjct: 150 RGVGLGMKHGARAMIKRGFKGCIISTASVAGVMGGMGPHAYTASKHAIVGLTKNAACELG 209

Query: 191 RYGIRVNCVSPNVVPTEMGRKLFK-----------LKEGDEFPSFYWNLKGGDILREEDV 250
           +YGIRVNC+SP  V T M    ++           ++E +EF     NLK G+ LR  D+
Sbjct: 210 KYGIRVNCISPFGVATSMLVNAWRKTSGGDVEDDDVEEMEEFVRSLANLK-GETLRANDI 269

Query: 251 GEAVVYLGSDESKCVSGLNLIVDGGFTVVNQAL 268
            EA +YL SDESK V+G NL+VDGG T     +
Sbjct: 270 AEAALYLASDESKYVNGHNLVVDGGVTTARNCV 301

BLAST of Bhi08M000017 vs. TAIR 10
Match: AT1G52340.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 232.3 bits (591), Expect = 4.8e-61
Identity = 133/281 (47.33%), Postives = 174/281 (61.92%), Query Frame = 0

Query: 2   SFQLLPAVARRLEGKVAVITGGARGIGEHTARLFLKHGAKVVIADIEDDLGHALCNDYLG 61
           S+  LP  ++RL GKVA+ITGGA GIGE   RLF KHGAKV I D++DDLG  +C   L 
Sbjct: 9   SYSSLP--SQRLLGKVALITGGATGIGESIVRLFHKHGAKVCIVDLQDDLGGEVCKSLLR 68

Query: 62  SSSSS---FVHCDVTKEKDVENAIDTAVSKYGKLDIMFNNAGVFDEASNFDILKDDPLTF 121
             S     F+H DV  E D+ NA+D AV  +G LDI+ NNAG+   A   DI       F
Sbjct: 69  GESKETAFFIHGDVRVEDDISNAVDFAVKNFGTLDILINNAGLCG-APCPDIRNYSLSEF 128

Query: 122 QKVVNVNLVGAFLGTKHAARVMKPCGRGSIVTTASICSVIGGIGTHAYTSSKHGVLGLMR 181
           +   +VN+ GAFL  KHAARVM P  +GSIV+  S+  V+GG+G H+Y  SKH VLGL R
Sbjct: 129 EMTFDVNVKGAFLSMKHAARVMIPEKKGSIVSLCSVGGVVGGVGPHSYVGSKHAVLGLTR 188

Query: 182 NTAVDLGRYGIRVNCVSPNVVPTEMGRKLFKLKEGDE--------FPSFYWNLKGGDILR 241
           + A +LG++GIRVNCVSP  V T++       +E  E        F +   NLKG + L 
Sbjct: 189 SVAAELGQHGIRVNCVSPYAVATKLALAHLPEEERTEDAFVGFRNFAAANANLKGVE-LT 248

Query: 242 EEDVGEAVVYLGSDESKCVSGLNLIVDGGFTVVNQALCSFQ 272
            +DV  AV++L SD+S+ +SG NL++DGGFT  N +   F+
Sbjct: 249 VDDVANAVLFLASDDSRYISGDNLMIDGGFTCTNHSFKVFR 285

BLAST of Bhi08M000017 vs. TAIR 10
Match: AT3G29250.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 217.6 bits (553), Expect = 1.2e-56
Identity = 124/255 (48.63%), Postives = 165/255 (64.71%), Query Frame = 0

Query: 12  RLEGKVAVITGGARGIGEHTARLFLKHGAKVVIADIEDDLGHALCNDYLGSSSSSFVHCD 71
           RL+GK+A+ITGGA GIG    RLF  HGAKVVI DI+++LG  L    +G   +SF  C+
Sbjct: 5   RLDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDIQEELGQNLAVS-IGLDKASFYRCN 64

Query: 72  VTKEKDVENAIDTAVSKYGKLDIMFNNAGVFDEASNFDILKDDPLTFQKVVNVNLVGAFL 131
           VT E DVENA+   V K+GKLD++F+NAGV +   +  +L  D   F + + VN+ GA  
Sbjct: 65  VTDETDVENAVKFTVEKHGKLDVLFSNAGVLEAFGS--VLDLDLEAFDRTMAVNVRGAAA 124

Query: 132 GTKHAARVMKPCG-RGSIVTTASICSVIGGIGTHAYTSSKHGVLGLMRNTAVDLGRYGIR 191
             KHAAR M   G RGSIV T SI + IGG G H+YT+SKH +LGL+R+    LG+YGIR
Sbjct: 125 FIKHAARSMVASGTRGSIVCTTSIAAEIGGPGPHSYTASKHALLGLIRSACAGLGQYGIR 184

Query: 192 VNCVSPNVVPTEMGRKLFK--LKEGDEFPSFYWNLKGGDILREEDVGEAVVYLGSDESKC 251
           VN V+P  V T M     +  +K  +E+     NLK G +L+   + EA ++L SD+S  
Sbjct: 185 VNGVAPYGVATGMTSAYNEEAVKMLEEYGEALGNLK-GVVLKARHIAEAALFLASDDSVY 244

Query: 252 VSGLNLIVDGGFTVV 264
           +SG NL+VDGGF+VV
Sbjct: 245 ISGQNLVVDGGFSVV 255

BLAST of Bhi08M000017 vs. TAIR 10
Match: AT2G47130.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 217.2 bits (552), Expect = 1.6e-56
Identity = 121/253 (47.83%), Postives = 161/253 (63.64%), Query Frame = 0

Query: 12  RLEGKVAVITGGARGIGEHTARLFLKHGAKVVIADIEDDLGHALCNDYLGSSSSSFVHCD 71
           RL+GK+A+ITGGA GIG    RLF  HGAKVVI D +++LG  +    +G   +SF  CD
Sbjct: 5   RLDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDFQEELGQNVAVS-VGKDKASFYRCD 64

Query: 72  VTKEKDVENAIDTAVSKYGKLDIMFNNAGVFDEASNFDILKDDPLTFQKVVNVNLVGAFL 131
           VT EK+VENA+   V KYGKLD++F+NAGV ++  +F  L  +   F + + VN+ GA  
Sbjct: 65  VTNEKEVENAVKFTVEKYGKLDVLFSNAGVMEQPGSFLDLNLE--QFDRTMAVNVRGAAA 124

Query: 132 GTKHAARVMKPCG-RGSIVTTASICSVIGGIGTHAYTSSKHGVLGLMRNTAVDLGRYGIR 191
             KHAAR M   G RGSIV T S+ S IGG G HAYT+SKH +LGL+++    LG+YGIR
Sbjct: 125 FIKHAARAMVEKGTRGSIVCTTSVASEIGGPGPHAYTASKHALLGLVKSACGGLGKYGIR 184

Query: 192 VNCVSPNVVPTEMGRKLFKLKEGDEFPSFYWNLKGGDILREEDVGEAVVYLGSDESKCVS 251
           VN V+P  V T +  +  +     E  S    +  G +L+   V EA ++L SD+S  VS
Sbjct: 185 VNGVAPYAVATAINSRDEETVRMVEEYSAATGILKGVVLKARHVAEAALFLASDDSAYVS 244

Query: 252 GLNLIVDGGFTVV 264
           G NL VDGG++VV
Sbjct: 245 GQNLAVDGGYSVV 254

BLAST of Bhi08M000017 vs. TAIR 10
Match: AT3G29250.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 215.7 bits (548), Expect = 4.6e-56
Identity = 123/254 (48.43%), Postives = 164/254 (64.57%), Query Frame = 0

Query: 13  LEGKVAVITGGARGIGEHTARLFLKHGAKVVIADIEDDLGHALCNDYLGSSSSSFVHCDV 72
           L+GK+A+ITGGA GIG    RLF  HGAKVVI DI+++LG  L    +G   +SF  C+V
Sbjct: 44  LDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDIQEELGQNLAVS-IGLDKASFYRCNV 103

Query: 73  TKEKDVENAIDTAVSKYGKLDIMFNNAGVFDEASNFDILKDDPLTFQKVVNVNLVGAFLG 132
           T E DVENA+   V K+GKLD++F+NAGV +   +  +L  D   F + + VN+ GA   
Sbjct: 104 TDETDVENAVKFTVEKHGKLDVLFSNAGVLEAFGS--VLDLDLEAFDRTMAVNVRGAAAF 163

Query: 133 TKHAARVMKPCG-RGSIVTTASICSVIGGIGTHAYTSSKHGVLGLMRNTAVDLGRYGIRV 192
            KHAAR M   G RGSIV T SI + IGG G H+YT+SKH +LGL+R+    LG+YGIRV
Sbjct: 164 IKHAARSMVASGTRGSIVCTTSIAAEIGGPGPHSYTASKHALLGLIRSACAGLGQYGIRV 223

Query: 193 NCVSPNVVPTEMGRKLFK--LKEGDEFPSFYWNLKGGDILREEDVGEAVVYLGSDESKCV 252
           N V+P  V T M     +  +K  +E+     NLK G +L+   + EA ++L SD+S  +
Sbjct: 224 NGVAPYGVATGMTSAYNEEAVKMLEEYGEALGNLK-GVVLKARHIAEAALFLASDDSVYI 283

Query: 253 SGLNLIVDGGFTVV 264
           SG NL+VDGGF+VV
Sbjct: 284 SGQNLVVDGGFSVV 293

BLAST of Bhi08M000017 vs. ExPASy Swiss-Prot
Match: Q94KL7 (Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 PE=1 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 8.8e-76
Identity = 147/271 (54.24%), Postives = 197/271 (72.69%), Query Frame = 0

Query: 4   QLLPAVARRLEGKVAVITGGARGIGEHTARLFLKHGAKVVIADIEDDLGHALCNDYLGSS 63
           Q+L A+ARRLEGKVA+ITGGA GIGE TA+LF +HGAKV IAD++D+LGH++  + +G+S
Sbjct: 6   QVLTAIARRLEGKVALITGGASGIGETTAKLFSQHGAKVAIADVQDELGHSVV-EAIGTS 65

Query: 64  SSSFVHCDVTKEKDVENAIDTAVSKYGKLDIMFNNAGVFDEASNFDILKDDPLTFQKVVN 123
           +S+++HCDVT E  V+NA+D  VS YGKLDIMF+NAG+ D  +   I+ ++   F++V +
Sbjct: 66  NSTYIHCDVTNEDGVKNAVDNTVSTYGKLDIMFSNAGISD-PNRPRIIDNEKADFERVFS 125

Query: 124 VNLVGAFLGTKHAARVMKPCGRGSIVTTASICSVIGGIGTHAYTSSKHGVLGLMRNTAVD 183
           VN+ G FL  KHAARVM P   G+I++TAS+ S +GG  +HAY  SKH VLGL RN AV+
Sbjct: 126 VNVTGVFLCMKHAARVMIPARSGNIISTASLSSTMGGGSSHAYCGSKHAVLGLTRNLAVE 185

Query: 184 LGRYGIRVNCVSPNVVPTEMGRKLFKLKEGDEFP---SFYWNLKGGDILREEDVGEAVVY 243
           LG++GIRVNC+SP  +PT +G+K   +K  +EF    +F  NLKG      EDV  A +Y
Sbjct: 186 LGQFGIRVNCLSPFGLPTALGKKFSGIKNEEEFENVINFAGNLKGPK-FNVEDVANAALY 245

Query: 244 LGSDESKCVSGLNLIVDGGFTVVNQALCSFQ 272
           L SDE+K VSG NL +DGGF+V N  +  FQ
Sbjct: 246 LASDEAKYVSGHNLFIDGGFSVCNSVIKVFQ 273

BLAST of Bhi08M000017 vs. ExPASy Swiss-Prot
Match: Q7FAE1 (Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 PE=2 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 1.0e-68
Identity = 140/266 (52.63%), Postives = 185/266 (69.55%), Query Frame = 0

Query: 8   AVARRLEGKVAVITGGARGIGEHTARLFLKHGAKVVIADIEDDLGHALCNDYLGSSSSSF 67
           A AR+L GKVAVITGGA GIG  TARLF+KHGA+VV+ADI+D+LG +L  + LG  +SS+
Sbjct: 10  ADARKLVGKVAVITGGASGIGACTARLFVKHGARVVVADIQDELGASLVAE-LGPDASSY 69

Query: 68  VHCDVTKEKDVENAIDTAVSKYGKLDIMFNNAGVFDEASNFDILKDDPLTFQKVVNVNLV 127
           VHCDVT E DV  A+D AV+++GKLD+MFNNAGV      F + +     F++V+ VNLV
Sbjct: 70  VHCDVTNEGDVAAAVDHAVARFGKLDVMFNNAGVSGPPC-FRMSECTKEDFERVLAVNLV 129

Query: 128 GAFLGTKHAARVMKPCGRGSIVTTASICSVIGGIGTHAYTSSKHGVLGLMRNTAVDLGRY 187
           G FLGTKHAARVM P  RGSI++TAS+ S + G  +HAYT+SKH ++G   N A +LGR+
Sbjct: 130 GPFLGTKHAARVMAPARRGSIISTASLSSSVSGAASHAYTTSKHALVGFTENAAGELGRH 189

Query: 188 GIRVNCVSPNVVPTEMGRKLFKLKEG--DEFPSFYWNLKGGDILREEDVGEAVVYLGSDE 247
           GIRVNCVSP  V T + R    + +   +   +   NLKG   L+ +D+  A ++L SD+
Sbjct: 190 GIRVNCVSPAGVATPLARAAMGMDDEAIEAIMANSANLKGAGALKADDIAAAALFLASDD 249

Query: 248 SKCVSGLNLIVDGGFTVVNQALCSFQ 272
            + VSG NL VDGG +VVN +   F+
Sbjct: 250 GRYVSGQNLRVDGGLSVVNSSFGFFR 273

BLAST of Bhi08M000017 vs. ExPASy Swiss-Prot
Match: Q94KL8 (Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 PE=1 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 8.8e-68
Identity = 138/258 (53.49%), Postives = 178/258 (68.99%), Query Frame = 0

Query: 12  RLEGKVAVITGGARGIGEHTARLFLKHGAKVVIADIEDDLGHALCNDYLGSSSSSFVHCD 71
           RL+ KVA+ITGGA GIGE TA+LF+++GAKVVIADI DD G  +CN+       SFVHCD
Sbjct: 13  RLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADIADDHGQKVCNNIGSPDVISFVHCD 72

Query: 72  VTKEKDVENAIDTAVSKYGKLDIMFNNAGVFDEASNFDILKDDPLTFQKVVNVNLVGAFL 131
           VTK++DV N +DT ++K+GKLDIMF N GV    + + IL+     F++V+++N+ GAFL
Sbjct: 73  VTKDEDVRNLVDTTIAKHGKLDIMFGNVGVL-STTPYSILEAGNEDFKRVMDINVYGAFL 132

Query: 132 GTKHAARVMKPCGRGSIVTTASICSVIGGIG-THAYTSSKHGVLGLMRNTAVDLGRYGIR 191
             KHAARVM P  +GSIV TASI S   G G +H YT++KH VLGL  +   +LG YGIR
Sbjct: 133 VAKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTTSLCTELGEYGIR 192

Query: 192 VNCVSPNVVPTEMGRKLFKLKEG--DEFPSFYWNLKGGDILREEDVGEAVVYLGSDESKC 251
           VNCVSP +V + +   +F +     +E      NLK G +LR EDV +AV YL  DESK 
Sbjct: 193 VNCVSPYIVASPLLTDVFGVDSSRVEELAHQAANLK-GTLLRAEDVADAVAYLAGDESKY 252

Query: 252 VSGLNLIVDGGFTVVNQA 267
           VSGLNL++DGG+T  N A
Sbjct: 253 VSGLNLVIDGGYTRTNPA 268

BLAST of Bhi08M000017 vs. ExPASy Swiss-Prot
Match: A3F5F0 (Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH PE=1 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 2.6e-67
Identity = 138/258 (53.49%), Postives = 179/258 (69.38%), Query Frame = 0

Query: 12  RLEGKVAVITGGARGIGEHTARLFLKHGAKVVIADIEDDLGHALCNDYLGSSSSSFVHCD 71
           RL+ KVA+ITGGA GIGE TA+LF+++GAKVVIADI DD G  +CN+       SFVHCD
Sbjct: 13  RLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADISDDHGQKVCNNIGSPDVISFVHCD 72

Query: 72  VTKEKDVENAIDTAVSKYGKLDIMFNNAGVFDEASNFDILKDDPLTFQKVVNVNLVGAFL 131
           VTK++DV N +DT ++K+GKLDIMF N GV    + + IL+     F++V+++N+ GAFL
Sbjct: 73  VTKDEDVRNLVDTTIAKHGKLDIMFGNVGVL-STTPYSILEAGNEDFKRVMDINVYGAFL 132

Query: 132 GTKHAARVMKPCGRGSIVTTASICSVIGGIG-THAYTSSKHGVLGLMRNTAVDLGRYGIR 191
             KHAARVM P  +GSIV TASI S   G G +H YT++KH VLGL  +   +LG++GIR
Sbjct: 133 VAKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTTSLCTELGQHGIR 192

Query: 192 VNCVSPNVVPTEMGRKLFKLKEG--DEFPSFYWNLKGGDILREEDVGEAVVYLGSDESKC 251
           VNCVSP VV + +   +F +     +E      NLK G +LR EDV +AV YL  DESK 
Sbjct: 193 VNCVSPYVVASPLLTDVFGVDSSRVEELAHQAANLK-GILLRAEDVADAVAYLAGDESKY 252

Query: 252 VSGLNLIVDGGFTVVNQA 267
           VSGLNL++DGG+T  N A
Sbjct: 253 VSGLNLVIDGGYTRTNPA 268

BLAST of Bhi08M000017 vs. ExPASy Swiss-Prot
Match: F1SWA0 (Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 2.2e-66
Identity = 133/268 (49.63%), Postives = 173/268 (64.55%), Query Frame = 0

Query: 12  RLEGKVAVITGGARGIGEHTARLFLKHGAKVVIADIEDDLGHALCNDYLGSSSSSFVHCD 71
           RLEGKVA++TGGA GIGE  ARLF++HGAK+ I D++D+LG  +     G   + + HCD
Sbjct: 2   RLEGKVALVTGGASGIGESIARLFIEHGAKICIVDVQDELGQQVSQRLGGDPHACYFHCD 61

Query: 72  VTKEKDVENAIDTAVSKYGKLDIMFNNAGVFDEASNFDILKDDPLTFQKVVNVNLVGAFL 131
           VT E DV  A+D    KYG +DIM NNAG+  +    DI   D   F+KV ++N+ G FL
Sbjct: 62  VTVEDDVRRAVDFTAEKYGTIDIMVNNAGITGD-KVIDIRDADFNEFKKVFDINVNGVFL 121

Query: 132 GTKHAARVMKPCGRGSIVTTASICSVIGGIGTHAYTSSKHGVLGLMRNTAVDLGRYGIRV 191
           G KHAAR+M P  +GSIV+ AS+ SVI G G H YT +KH V+GL ++ A +LGR+GIRV
Sbjct: 122 GMKHAARIMIPKMKGSIVSLASVSSVIAGAGPHGYTGAKHAVVGLTKSVAAELGRHGIRV 181

Query: 192 NCVSPNVVPTEMGRKLFKLKEGDE--------FPSFYWNLKGGDILREEDVGEAVVYLGS 251
           NCVSP  VPT +        E  E        F     NLKG D++   DV EAV+YL +
Sbjct: 182 NCVSPYAVPTRLSMPYLPESEMQEDALRGFLTFVRSNANLKGVDLM-PNDVAEAVLYLAT 241

Query: 252 DESKCVSGLNLIVDGGFTVVNQALCSFQ 272
           +ESK VSGLNL++DGGF++ N  L  F+
Sbjct: 242 EESKYVSGLNLVIDGGFSIANHTLQVFE 267

BLAST of Bhi08M000017 vs. ExPASy TrEMBL
Match: A0A0A0LVV9 (TASSELSEED2-like protein OS=Cucumis sativus OX=3659 GN=Csa_1G168880 PE=4 SV=1)

HSP 1 Score: 490.7 bits (1262), Expect = 3.9e-135
Identity = 243/271 (89.67%), Postives = 254/271 (93.73%), Query Frame = 0

Query: 1   MSFQLLPAVARRLEGKVAVITGGARGIGEHTARLFLKHGAKVVIADIEDDLGHALCNDYL 60
           MS QLLPA+ARRLEGKVAVITGGARGIGE TA+LF KHGAKVVIADI+D LG  LC D L
Sbjct: 1   MSIQLLPAIARRLEGKVAVITGGARGIGEQTAKLFFKHGAKVVIADIQDHLGQTLCKD-L 60

Query: 61  GSSSSSFVHCDVTKEKDVENAIDTAVSKYGKLDIMFNNAGVFDEASNFDILKDDPLTFQK 120
           G SSS FVHCDVTKEKDVE A+DTAVSKYGKLDIM NNAGVF+E+ NFDILKDDPLTFQ+
Sbjct: 61  GQSSSVFVHCDVTKEKDVETAVDTAVSKYGKLDIMLNNAGVFEESPNFDILKDDPLTFQR 120

Query: 121 VVNVNLVGAFLGTKHAARVMKPCGRGSIVTTASICSVIGGIGTHAYTSSKHGVLGLMRNT 180
           VVNVNLVGAFLGTKHAARVMKP GRGSIVTTASICSVIGGIGTHAYTSSKHGVLGLMRN 
Sbjct: 121 VVNVNLVGAFLGTKHAARVMKPAGRGSIVTTASICSVIGGIGTHAYTSSKHGVLGLMRNA 180

Query: 181 AVDLGRYGIRVNCVSPNVVPTEMGRKLFKLKEGDEFPSFYWNLKGGDILREEDVGEAVVY 240
           AVDLGRYGIRVNCVSPNVVPTEMGRKLFK+K+G EFPSFYW+LK GDILREEDVGEAVVY
Sbjct: 181 AVDLGRYGIRVNCVSPNVVPTEMGRKLFKVKDGGEFPSFYWSLKNGDILREEDVGEAVVY 240

Query: 241 LGSDESKCVSGLNLIVDGGFTVVNQALCSFQ 272
           LGSDESKCVSGLNLIVDGGFTVVNQALCSF+
Sbjct: 241 LGSDESKCVSGLNLIVDGGFTVVNQALCSFR 270

BLAST of Bhi08M000017 vs. ExPASy TrEMBL
Match: Q94G09 (TASSELSEED2-like protein OS=Cucumis sativus OX=3659 GN=CTS2 PE=2 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 1.1e-134
Identity = 242/271 (89.30%), Postives = 253/271 (93.36%), Query Frame = 0

Query: 1   MSFQLLPAVARRLEGKVAVITGGARGIGEHTARLFLKHGAKVVIADIEDDLGHALCNDYL 60
           MS QLLPA+ARRLEGKVAVITGGARGIGE TA+LF KHGAKVVIADI+D LG  LC D L
Sbjct: 1   MSIQLLPAIARRLEGKVAVITGGARGIGEQTAKLFFKHGAKVVIADIQDHLGQTLCKD-L 60

Query: 61  GSSSSSFVHCDVTKEKDVENAIDTAVSKYGKLDIMFNNAGVFDEASNFDILKDDPLTFQK 120
           G SSS FVHCDVTKEKDVE A+DTAVSKYGKLDIM NNAGVF+E+ NFD LKDDPLTFQ+
Sbjct: 61  GQSSSVFVHCDVTKEKDVETAVDTAVSKYGKLDIMLNNAGVFEESPNFDFLKDDPLTFQR 120

Query: 121 VVNVNLVGAFLGTKHAARVMKPCGRGSIVTTASICSVIGGIGTHAYTSSKHGVLGLMRNT 180
           VVNVNLVGAFLGTKHAARVMKP GRGSIVTTASICSVIGGIGTHAYTSSKHGVLGLMRN 
Sbjct: 121 VVNVNLVGAFLGTKHAARVMKPAGRGSIVTTASICSVIGGIGTHAYTSSKHGVLGLMRNA 180

Query: 181 AVDLGRYGIRVNCVSPNVVPTEMGRKLFKLKEGDEFPSFYWNLKGGDILREEDVGEAVVY 240
           AVDLGRYGIRVNCVSPNVVPTEMGRKLFK+K+G EFPSFYW+LK GDILREEDVGEAVVY
Sbjct: 181 AVDLGRYGIRVNCVSPNVVPTEMGRKLFKVKDGGEFPSFYWSLKNGDILREEDVGEAVVY 240

Query: 241 LGSDESKCVSGLNLIVDGGFTVVNQALCSFQ 272
           LGSDESKCVSGLNLIVDGGFTVVNQALCSF+
Sbjct: 241 LGSDESKCVSGLNLIVDGGFTVVNQALCSFR 270

BLAST of Bhi08M000017 vs. ExPASy TrEMBL
Match: Q94G10 (CTA OS=Cucumis sativus OX=3659 GN=CTA PE=2 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 7.3e-134
Identity = 241/271 (88.93%), Postives = 253/271 (93.36%), Query Frame = 0

Query: 1   MSFQLLPAVARRLEGKVAVITGGARGIGEHTARLFLKHGAKVVIADIEDDLGHALCNDYL 60
           MS QLLPA+ARRLEGKVAVITGGARGIGE TA+LF KHGAKVVIADI+D LG  LC D L
Sbjct: 1   MSIQLLPAIARRLEGKVAVITGGARGIGEQTAKLFFKHGAKVVIADIQDHLGQTLCKD-L 60

Query: 61  GSSSSSFVHCDVTKEKDVENAIDTAVSKYGKLDIMFNNAGVFDEASNFDILKDDPLTFQK 120
           G SSS FVHCDVTKEKDVE A+DTAVSKYGKLDIM NNAGVF+E+ NFDILKDDPLTFQ+
Sbjct: 61  GQSSSVFVHCDVTKEKDVETAVDTAVSKYGKLDIMLNNAGVFEESPNFDILKDDPLTFQR 120

Query: 121 VVNVNLVGAFLGTKHAARVMKPCGRGSIVTTASICSVIGGIGTHAYTSSKHGVLGLMRNT 180
           VVNVNLVGA LGT+HAARVMKP GRGSIVTTASICSVIGGIGTHAYTSSKHGVLGLMRN 
Sbjct: 121 VVNVNLVGASLGTRHAARVMKPAGRGSIVTTASICSVIGGIGTHAYTSSKHGVLGLMRNA 180

Query: 181 AVDLGRYGIRVNCVSPNVVPTEMGRKLFKLKEGDEFPSFYWNLKGGDILREEDVGEAVVY 240
           AVDLGRYGIRVNCVSPNVVPTEMGRKLFK+K+G EFPSFYW+LK GDILREEDVGEAVVY
Sbjct: 181 AVDLGRYGIRVNCVSPNVVPTEMGRKLFKVKDGGEFPSFYWSLKNGDILREEDVGEAVVY 240

Query: 241 LGSDESKCVSGLNLIVDGGFTVVNQALCSFQ 272
           LGSDESKCVSGLNLIVDGGFTVVNQALCSF+
Sbjct: 241 LGSDESKCVSGLNLIVDGGFTVVNQALCSFR 270

BLAST of Bhi08M000017 vs. ExPASy TrEMBL
Match: A0A1S4E490 (secoisolariciresinol dehydrogenase-like OS=Cucumis melo OX=3656 GN=LOC103501741 PE=4 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 4.3e-110
Identity = 201/231 (87.01%), Postives = 212/231 (91.77%), Query Frame = 0

Query: 1   MSFQLLPAVARRLEGKVAVITGGARGIGEHTARLFLKHGAKVVIADIEDDLGHALCNDYL 60
           MS QLLPA+ARRLEGKVAVITGGARGIGE TA+LF KHGAKVVIADI+DDLG  LC D L
Sbjct: 1   MSIQLLPAIARRLEGKVAVITGGARGIGEQTAKLFFKHGAKVVIADIQDDLGQKLCKD-L 60

Query: 61  GSSSSSFVHCDVTKEKDVENAIDTAVSKYGKLDIMFNNAGVFDEASNFDILKDDPLTFQK 120
           G SSS+FVHCDVTKEKDVE A+D AVSKYGKLDIM NNAGVF+EA NFDILKDDPLTFQ+
Sbjct: 61  GQSSSAFVHCDVTKEKDVETAVDMAVSKYGKLDIMLNNAGVFEEAPNFDILKDDPLTFQR 120

Query: 121 VVNVNLVGAFLGTKHAARVMKPCGRGSIVTTASICSVIGGIGTHAYTSSKHGVLGLMRNT 180
           VVNVNLVGAFLG KHAARVMKP GRGSI+TTASICSVIGGIGTHAYTSSKHGVLGL RN 
Sbjct: 121 VVNVNLVGAFLGMKHAARVMKPAGRGSIITTASICSVIGGIGTHAYTSSKHGVLGLTRNA 180

Query: 181 AVDLGRYGIRVNCVSPNVVPTEMGRKLFKLKEGDEFPSFYWNLKGGDILRE 232
           AVDLGRYGIRVNCVSPNVVPTEMGRKL K+K+G EFPSFYW+LK GDILRE
Sbjct: 181 AVDLGRYGIRVNCVSPNVVPTEMGRKLLKVKDGGEFPSFYWSLKNGDILRE 230

BLAST of Bhi08M000017 vs. ExPASy TrEMBL
Match: A0A6J1CMN7 (momilactone A synthase-like OS=Momordica charantia OX=3673 GN=LOC111012546 PE=4 SV=1)

HSP 1 Score: 394.0 bits (1011), Expect = 5.0e-106
Identity = 199/273 (72.89%), Postives = 229/273 (83.88%), Query Frame = 0

Query: 1   MSFQLLPAVARRLEGKVAVITGGARGIGEHTARLFLKHGAKVVIADIEDDLGHALCNDYL 60
           M   LLPA ARRLEGKVA+ITGGARGIGE+TARLF KHGAKVVIADI+DDLGH+L N+ L
Sbjct: 1   MGVPLLPAAARRLEGKVALITGGARGIGEYTARLFFKHGAKVVIADIQDDLGHSLSNN-L 60

Query: 61  GSSSSSFVHCDVTKEKDVENAIDTAVSKYGKLDIMFNNAGVFDEASNFDILKDDPLTFQK 120
             SSSSFVHCDVT+E DV NA+DT VSKYGKLDIMFNNAG+  +   F+IL+++ L FQK
Sbjct: 61  CPSSSSFVHCDVTRENDVANAVDTTVSKYGKLDIMFNNAGISGDL-KFNILENESLDFQK 120

Query: 121 VVNVNLVGAFLGTKHAARVMKPCGRGSIVTTASICSVIGGIGTHAYTSSKHGVLGLMRNT 180
           V+NVNL+GAFLGTKHAAR M P  RGSI+ TAS CSVIGG   HAYTSSKHG++GLM+N 
Sbjct: 121 VLNVNLLGAFLGTKHAARAMMPARRGSIIMTASGCSVIGGACPHAYTSSKHGMVGLMKNA 180

Query: 181 AVDLGRYGIRVNCVSPNVVPTEMGRKLFKLKEGDEFPSFYWNLKGGDILREEDVGEAVVY 240
           AVDLGRYGIRVNCVSP+VVPT+M R LFKL++GDEFP+ Y +LK G +L EED+ EA +Y
Sbjct: 181 AVDLGRYGIRVNCVSPHVVPTQMTRDLFKLEDGDEFPNHYSHLKCGGMLMEEDIAEAALY 240

Query: 241 LGSDESKCVSGLNLIVDGGFTVVNQALCSFQPS 274
           LGSD SK VSG NLIVDGGFTVVN+A CS+  S
Sbjct: 241 LGSDASKFVSGHNLIVDGGFTVVNRAFCSYDQS 271

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT3G51680.12.5e-6247.25NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G52340.14.8e-6147.33NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G29250.21.2e-5648.63NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G47130.11.6e-5647.83NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G29250.14.6e-5648.43NAD(P)-binding Rossmann-fold superfamily protein [more]
Match NameE-valueIdentityDescription
Q94KL78.8e-7654.24Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 P... [more]
Q7FAE11.0e-6852.63Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 ... [more]
Q94KL88.8e-6853.49Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 P... [more]
A3F5F02.6e-6753.49Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH ... [more]
F1SWA02.2e-6649.63Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LVV93.9e-13589.67TASSELSEED2-like protein OS=Cucumis sativus OX=3659 GN=Csa_1G168880 PE=4 SV=1[more]
Q94G091.1e-13489.30TASSELSEED2-like protein OS=Cucumis sativus OX=3659 GN=CTS2 PE=2 SV=1[more]
Q94G107.3e-13488.93CTA OS=Cucumis sativus OX=3659 GN=CTA PE=2 SV=1[more]
A0A1S4E4904.3e-11087.01secoisolariciresinol dehydrogenase-like OS=Cucumis melo OX=3656 GN=LOC103501741 ... [more]
A0A6J1CMN75.0e-10672.89momilactone A synthase-like OS=Momordica charantia OX=3673 GN=LOC111012546 PE=4 ... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 166..185
score: 31.56
coord: 90..101
score: 47.7
coord: 146..154
score: 40.77
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 90..101
score: 55.71
coord: 140..156
score: 30.61
coord: 187..204
score: 41.74
coord: 166..185
score: 36.22
coord: 224..244
score: 26.03
coord: 17..34
score: 48.39
NoneNo IPR availablePFAMPF13561adh_short_C2coord: 22..261
e-value: 1.4E-62
score: 211.3
NoneNo IPR availableGENE3D3.40.50.720coord: 7..271
e-value: 1.8E-74
score: 252.2
NoneNo IPR availablePIRSRPIRSR000094-2PIRSR000094-2coord: 13..264
e-value: 5.7E-16
score: 56.0
NoneNo IPR availablePIRSRPIRSR000095-1PIRSR000095-1coord: 19..214
e-value: 6.0E-17
score: 59.3
NoneNo IPR availablePANTHERPTHR43180:SF30MOMILACTONE A SYNTHASEcoord: 7..266
NoneNo IPR availablePANTHERPTHR431803-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)coord: 7..266
IPR020904Short-chain dehydrogenase/reductase, conserved sitePROSITEPS00061ADH_SHORTcoord: 153..181
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 14..263

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Bhi08G000017Bhi08G000017gene


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Bhi08M000017.utr5p1Bhi08M000017.utr5p1five_prime_UTR


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Bhi08M000017.exon1Bhi08M000017.exon1exon
Bhi08M000017.exon2Bhi08M000017.exon2exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
cds.Bhi08M000017cds.Bhi08M000017CDS
cds.Bhi08M000017cds.Bhi08M000017_2CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Bhi08M000017.utr3p1Bhi08M000017.utr3p1three_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Bhi08M000017Bhi08M000017-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016491 oxidoreductase activity