Tan0022462 (gene) Snake gourd v1

Overview
NameTan0022462
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionHydroxyisourate hydrolase
LocationLG10: 3387540 .. 3391562 (-)
RNA-Seq ExpressionTan0022462
SyntenyTan0022462
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAACACACCACGCAGCAGCAGAATTGATTGATTCAACGAAACATCGGCTGTTGAGTGGGGCTTAGAGACTCCGACTGCACACAGGAAAGAGCGAGAAAGAGAGAGAGAGAGAAGAGTGTGACTGGTGATCACTGATCGGCGGCAGTAACGATGGCGTCGAATTATGTTTTTGGAGAGGAAGAGTTTCAGGCATGCTGTGGAAGCTCCAAATTCGCCAGAGAAATGGTTTCCGTTTCCCCTTTTTTCTCCTTGGAACAAACTGTAGCTGCAGCTAGATACATCTGGTTCAATCAGGTCTCCATTTTCTTTTACCTTCTTTAGTCTCTTTGTATCATTGATTGTCTGTGATTAATCGAGGCTGAAATTTGATGTTCGTTGTTCAAAGGTCGATGTGAATGGTTGGCTGGAAGCATTCTCTGCTCATCCACAGATTGGTCAAGCCTCCAAATCATCCAATCAGACGAGTGCTCAGTTAAGATCCTTTTAATGTTAGATTCTTACTTCTCTTTTTTGTCAAATTGAATTGATTGGATCACTTTTAAGTTCTGGTAGCTTACAGTTATTTGAACAGAACTAGGAATAAGATTGTATTCTATCAGTCGCTTTCCAGTGGATGCAATTGATTTCTGGAACTTAGAACTTGATTTTTCTTAATTCACCAATCAAACCAAAAGCTTAAACTTATAGTTTGGGGTAAATTTAATTATATCAAGATGTCAAAGACTCTCCAACGTGTGCGCTTGGAAATTTGATAAAAAACCAAACACGTGAAATCAATATTAAACGGGAGAGGAAACTGACTTTGCATGAGTTTGAATACATGAACTCCTAGACCACCTGCTCTATAACATCTTAAATCACCAATCAAACCAAAAGCTTAAGCTGATAGTTTGGGGTAAATTTAATTAGATCAAGATGTCTAACAATTTTAAGTTATCCGAATAGAACTTGGTTTTAATTTTATGATTTTCTTTTAATTGTTGATTTCCAGTTTTGTGTTTGTGAAGATGTTCTAATTATGACGGTTGGTAATTGGCTTGCGACTTCTTCAATTAGCTAGTAGGAGTAGTTTAGTCCGTATATTCTCAGATGATATTTGAACTGTCCGACTCTAAAGTGTGTTTCATTCTTACATTGGCTTAGAAGTCCTATGCATTAATTTTTTTTTTTCTTTTCATTTTTAACCTTTCTATTGTTCTGACTGTAGTTCTAAATAGTAGTTACTGCGATGGTCTTCACTGATCGGCATTAGTTCTCAGGTTGTTTCGGAAATTTAATTTAATTTTGTTATGTGTGATTAACTCTTTTGAAGAAAAAAAATGTGAGCATAATTTGGCTATCAAGTGAAATTGGGAGACTAACCATCATGGATTGGCGTATTGGTAACGGGTTCAAACGGCGGTGACCACCTATTTAGGATTTAATATTCTACAAGTTACCTTGACAACCAAATATAGTAGGGGTGTGATTGTCCCATGAGATTTATCAGATGCGCACAAGTTGGCTTGGACACTCACGGATATAAAAAAGGAGAAAAAGAAGGAAACTGGGAGACTTAGGTTTCGTTTAACTTTGTATGCTTTTGCAACTTGTACACTTGAAGTGACTTTTTAAATGAACATTTTCATAGATGGAGTAAGGGAGAACAGTCAACTGCACTGGCAACCGCTACTGGTTCTAGTTTACAGGTTAGTATCTGGAGTTTGTTTTAATATCATTAAATTGCCTAATATCATTCAATTAACTTTTTTTATTTTCTTTTGGGGGGTGGGGTTTGGACAAATTATTTGATGAGGTTGGCTCACAATAACCATTAGACGTGGATTGGATGATTTTTGTACCTCTAATAATCACTAGATATAAATTTGAGGTATTTTTCTCTATACATCTGACGGTGACTAGTAACCACATTGTGGCGGCATCACCCAATAATTTCTTTGGGGTTTTTTGGAAGTAGGGAAGAGGGGCAGGTTTGTGAGTGATGATTTATTGACCATGACTTCCAAGGTGTATTTCATAATACCTGGTGGCCTTTGCAGGAGCTAGCTGAATGGAATATTCAGTATCAGCAAAAGTTTGGATTTGTATTTCTGATTTGTGCATCTGGAAGGAGCACTAACGAAATACTTGCAGAATTGAAGGTAGATCCTTCATTGAAGTTCATTTTTATGATGACGTTTTCTGAAATAGCTTCTATTCATTCTTAACCTTTTTGAATCAGAATAATTTCAAACCATACAACAGACTAATCTGTCTAGAACATATTGTGATCACGCTAGCAACCATTATCTGAAGACATTGTTCAGATAGAGGATTGCCATATTCTTGGTGTTTGTTCTGTCTTTGGCATATCTTTTCTTCGACCTTGAGTAACTAAATTCAATTAGATAGAAACCTTTTTCTTCCTTATCAAGAGCTTCTGTACATTTTTGAAGCCAAGCTCAACGTTGAGAAGCTGTATCATATTGAATTGCAATGTTGGTTACTTTTGATTATATTCTGTTCATATTTTTCTGGCCAGAGGTGCAAGCCTTATGATATCTTTGTCGGTCATTGCTTAGAAATCAACTAGGTAATCGGTTTTGTTGTTGGTTGGAAGTTTGAAGTATGTTCCATTTGTTCTAAAACTTCATATGATCCCCCCGTTCATTCAGTCAAATATGTTTTCATCATCTTTTTTGAGCAATTGTGCGGTTGTGCCTGCTGCAGAAACGGTATTCAAACAGACCCATAGTTGAGTTTGAGATTGCTGCTCAGGAGCAAATGAAAATAACAGAGCTGCGTCTTGCAAAGCTTTTTTCAACCAAACAAAATACCTCTTCAACAAGAGAGAGTCATCCTCCAGCTTTTGCACGAAAAGTAGAAGGTAATCTTCGTTATGGAGATAACAAAAAAACTTGCAAATAGTACTTTTTCTGTTTCATCTTAGTGTGTTCATATCTTCATGGATATTCATCTTTAAACTTTCATACTTGTGATATTATCCGTCTCCAGTGAACAGTAACATTCATTAGTGAAATACAGAGTCACAAAAAACCAGCATGTTCAACTTAACAGAAACTAGTTTTCTCGCTTAATCTTTCTATTAAGAATGCAAAAAACAAAGAGATGAACAGATTAAAACACAGCTTCTCAGAAGAAATGAATGCTAAAACGAACACAAATTCTCCTAAGAGGAAACTGTTTGGTTGCTAGTAATAACTGTCGGTATCGAACTTCTTAATTCTTTGCACTCCAAGTAACAACTATTCTTCGTGATCTCGAATTTCAACTCTTAAGCTGGATGGATGAATATGGCATAATCATTTCTGAAAATAATAGATTGATTACTATATCCTGGTAACACTTTCTCATTTGTTCATCTTCAAACTCTAGAAGATCGTGTGAGCATTATTGAAGGACATCTAAGTGCTACTTCTGAAGCTTCAACTGGAAAAACATCACAAGTTTTAACTCGAACTCGTCCACCAATTACAACCCACGTGTTGGATGTTGCACGAGGATCTCCAGCTGCAGGTATTGACGTCCGTTTGGAAAGGTGGATTGGTACTCAACCGCGCCCCTTATTCGGCGAGGTCGACGTTGGTGGCTGGGTACTTGAAGGGTCTTCAGCAACAGATAAAGATGGCAGAAGTGGCCAATTGTTGAACATGATTGAAGCTATAAATCCAGGAATTTACAGGATAAGTTTTAACACAGGCAAGTATTGCCCCGCCGGGTTCTTTCCTTACGTTTCGATCGTGTTCGAAATCAGGGAATCCCAGAAGCTGGAACATTTCCATGTCCCTCTCTTGTTTTCACCATTCTCATTTTCCACCTATCGTGGAAGCTAGGAGTTTACTGCTCATGTGAGTGTTTCTGTCATTTATCTGGATGTCCTTCGGAGTACTCAGCAAAAGTCGAATAAAGCGATGTCGACTAATACTAGACGATGTTGAATGTATCGAATGTTGTATAACATGGACAAACTCTATGCAAATTACCTCATAAATATGAAGATATCGTCGATACATGCCTTTTG

mRNA sequence

AAAAACACACCACGCAGCAGCAGAATTGATTGATTCAACGAAACATCGGCTGTTGAGTGGGGCTTAGAGACTCCGACTGCACACAGGAAAGAGCGAGAAAGAGAGAGAGAGAGAAGAGTGTGACTGGTGATCACTGATCGGCGGCAGTAACGATGGCGTCGAATTATGTTTTTGGAGAGGAAGAGTTTCAGGCATGCTGTGGAAGCTCCAAATTCGCCAGAGAAATGGTTTCCGTTTCCCCTTTTTTCTCCTTGGAACAAACTGTAGCTGCAGCTAGATACATCTGGTTCAATCAGGTCGATGTGAATGGTTGGCTGGAAGCATTCTCTGCTCATCCACAGATTGGTCAAGCCTCCAAATCATCCAATCAGACGAGTGCTCAATGGAGTAAGGGAGAACAGTCAACTGCACTGGCAACCGCTACTGGTTCTAGTTTACAGGAGCTAGCTGAATGGAATATTCAGTATCAGCAAAAGTTTGGATTTGTATTTCTGATTTGTGCATCTGGAAGGAGCACTAACGAAATACTTGCAGAATTGAAGAAACGGTATTCAAACAGACCCATAGTTGAGTTTGAGATTGCTGCTCAGGAGCAAATGAAAATAACAGAGCTGCGTCTTGCAAAGCTTTTTTCAACCAAACAAAATACCTCTTCAACAAGAGAGAGTCATCCTCCAGCTTTTGCACGAAAAGTAGAAGATCGTGTGAGCATTATTGAAGGACATCTAAGTGCTACTTCTGAAGCTTCAACTGGAAAAACATCACAAGTTTTAACTCGAACTCGTCCACCAATTACAACCCACGTGTTGGATGTTGCACGAGGATCTCCAGCTGCAGGTATTGACGTCCGTTTGGAAAGGTGGATTGGTACTCAACCGCGCCCCTTATTCGGCGAGGTCGACGTTGGTGGCTGGGTACTTGAAGGGTCTTCAGCAACAGATAAAGATGGCAGAAGTGGCCAATTGTTGAACATGATTGAAGCTATAAATCCAGGAATTTACAGGATAAGTTTTAACACAGGCAAGTATTGCCCCGCCGGGTTCTTTCCTTACGTTTCGATCGTGTTCGAAATCAGGGAATCCCAGAAGCTGGAACATTTCCATGTCCCTCTCTTGTTTTCACCATTCTCATTTTCCACCTATCGTGGAAGCTAGGAGTTTACTGCTCATGTGAGTGTTTCTGTCATTTATCTGGATGTCCTTCGGAGTACTCAGCAAAAGTCGAATAAAGCGATGTCGACTAATACTAGACGATGTTGAATGTATCGAATGTTGTATAACATGGACAAACTCTATGCAAATTACCTCATAAATATGAAGATATCGTCGATACATGCCTTTTG

Coding sequence (CDS)

ATGGCGTCGAATTATGTTTTTGGAGAGGAAGAGTTTCAGGCATGCTGTGGAAGCTCCAAATTCGCCAGAGAAATGGTTTCCGTTTCCCCTTTTTTCTCCTTGGAACAAACTGTAGCTGCAGCTAGATACATCTGGTTCAATCAGGTCGATGTGAATGGTTGGCTGGAAGCATTCTCTGCTCATCCACAGATTGGTCAAGCCTCCAAATCATCCAATCAGACGAGTGCTCAATGGAGTAAGGGAGAACAGTCAACTGCACTGGCAACCGCTACTGGTTCTAGTTTACAGGAGCTAGCTGAATGGAATATTCAGTATCAGCAAAAGTTTGGATTTGTATTTCTGATTTGTGCATCTGGAAGGAGCACTAACGAAATACTTGCAGAATTGAAGAAACGGTATTCAAACAGACCCATAGTTGAGTTTGAGATTGCTGCTCAGGAGCAAATGAAAATAACAGAGCTGCGTCTTGCAAAGCTTTTTTCAACCAAACAAAATACCTCTTCAACAAGAGAGAGTCATCCTCCAGCTTTTGCACGAAAAGTAGAAGATCGTGTGAGCATTATTGAAGGACATCTAAGTGCTACTTCTGAAGCTTCAACTGGAAAAACATCACAAGTTTTAACTCGAACTCGTCCACCAATTACAACCCACGTGTTGGATGTTGCACGAGGATCTCCAGCTGCAGGTATTGACGTCCGTTTGGAAAGGTGGATTGGTACTCAACCGCGCCCCTTATTCGGCGAGGTCGACGTTGGTGGCTGGGTACTTGAAGGGTCTTCAGCAACAGATAAAGATGGCAGAAGTGGCCAATTGTTGAACATGATTGAAGCTATAAATCCAGGAATTTACAGGATAAGTTTTAACACAGGCAAGTATTGCCCCGCCGGGTTCTTTCCTTACGTTTCGATCGTGTTCGAAATCAGGGAATCCCAGAAGCTGGAACATTTCCATGTCCCTCTCTTGTTTTCACCATTCTCATTTTCCACCTATCGTGGAAGCTAG

Protein sequence

MASNYVFGEEEFQACCGSSKFAREMVSVSPFFSLEQTVAAARYIWFNQVDVNGWLEAFSAHPQIGQASKSSNQTSAQWSKGEQSTALATATGSSLQELAEWNIQYQQKFGFVFLICASGRSTNEILAELKKRYSNRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPAFARKVEDRVSIIEGHLSATSEASTGKTSQVLTRTRPPITTHVLDVARGSPAAGIDVRLERWIGTQPRPLFGEVDVGGWVLEGSSATDKDGRSGQLLNMIEAINPGIYRISFNTGKYCPAGFFPYVSIVFEIRESQKLEHFHVPLLFSPFSFSTYRGS
Homology
BLAST of Tan0022462 vs. ExPASy Swiss-Prot
Match: Q9LVM5 (Uric acid degradation bifunctional protein TTL OS=Arabidopsis thaliana OX=3702 GN=TTL PE=1 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 8.7e-102
Identity = 194/327 (59.33%), Postives = 240/327 (73.39%), Query Frame = 0

Query: 8   GEEEFQACCGSSKFAREMVSVSPFFSLEQTVAAARYIWFNQVDVNGWLEAFSAHPQIGQA 67
           GE+E++ CCGSS+FA++M +  P  S ++ +  AR IWFNQV+V  WLEAFSAHPQIG  
Sbjct: 6   GEDEWKVCCGSSEFAKQMSTSGPLTS-QEAIYTARDIWFNQVNVTDWLEAFSAHPQIGNT 65

Query: 68  -SKSSNQTSAQWSKGEQSTALATATGSSLQELAEWNIQYQQKFGFVFLICASGRSTNEIL 127
            S S N   A+ S  EQSTA AT + S+LQELAEWN+ Y++KFGF+F+ICASGR+  E+L
Sbjct: 66  PSPSINSDFARRSVSEQSTAFATTSASALQELAEWNVLYKKKFGFIFIICASGRTHAEML 125

Query: 128 AELKKRYSNRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPAFARKVEDRVS 187
             LK+RY NRPIVE EIAA EQMKITELR+AKLFS K    S  +S     + K +DR+ 
Sbjct: 126 HALKERYENRPIVELEIAAMEQMKITELRMAKLFSDKAKVISETDSSSSPVSTKPQDRLR 185

Query: 188 IIEGHLSATSEASTGKTSQVLTRTRPPITTHVLDVARGSPAAGIDVRLERWIGTQPRPLF 247
           II GHL+  +EA   K      R+RPPITTHVLDV+RG+PAAG++V LE W GT   P F
Sbjct: 186 IIGGHLNVAAEAKAPK------RSRPPITTHVLDVSRGAPAAGVEVHLEVWSGT-TGPSF 245

Query: 248 GEVDVGGWVLEGSSATDKDGRSGQLLNMIEAINPGIYRISFNTGKYCPAGFFPYVSIVFE 307
                G W   G+SATD+DGRSG L+++++A+NPG YRISF+T KY P  FFPYVSIVF+
Sbjct: 246 VHGGGGVWSSVGTSATDRDGRSGPLMDLVDALNPGTYRISFDTAKYSPGCFFPYVSIVFQ 305

Query: 308 IRESQKLEHFHVPLLFSPFSFSTYRGS 334
           + ESQK EHFHVPLL +PFSFSTYRGS
Sbjct: 306 VTESQKWEHFHVPLLLAPFSFSTYRGS 324

BLAST of Tan0022462 vs. ExPASy Swiss-Prot
Match: Q9CRB3 (5-hydroxyisourate hydrolase OS=Mus musculus OX=10090 GN=Urah PE=1 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 1.5e-16
Identity = 55/126 (43.65%), Postives = 73/126 (57.94%), Query Frame = 0

Query: 213 PITTHVLDVARGSPAAGIDVRLERWIGTQPRPLFGEVDVGGWVLEGSSATDKDGRSGQLL 272
           P+TTHVLD A G PA G+ +RL R           E     W+   +S T+ DGR   LL
Sbjct: 7   PLTTHVLDTASGLPAQGLCLRLSRL----------EAPCQQWMELRTSYTNLDGRCPGLL 66

Query: 273 NMIEAINPGIYRISFNTGKYC----PAGFFPYVSIVFEI-RESQKLEHFHVPLLFSPFSF 332
              + I PG Y++ F+T +Y        F+PYV +VF I +E+QK   FHVPLL SP+S+
Sbjct: 67  TPSQ-IKPGTYKLFFDTERYWKERGQESFYPYVEVVFTITKETQK---FHVPLLLSPWSY 118

Query: 333 STYRGS 334
           +TYRGS
Sbjct: 127 TTYRGS 118

BLAST of Tan0022462 vs. ExPASy Swiss-Prot
Match: Q06S87 (5-hydroxyisourate hydrolase OS=Danio rerio OX=7955 GN=urah PE=1 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 2.8e-15
Identity = 55/155 (35.48%), Postives = 79/155 (50.97%), Query Frame = 0

Query: 183 DRVSIIEGHLSATSEASTGKTSQVLTRTRPPITTHVLDVARGSPAAGIDVRLERWIGTQP 242
           +R+  I GH+      S  K   +      P++THVL++A+G P A + + L R      
Sbjct: 2   NRLQHIRGHI-----VSADKHINMSATLLSPLSTHVLNIAQGVPGANMTIVLHRL----- 61

Query: 243 RPLFGEVDVGGWVLEGSSATDKDGRSGQLLNMIEAINPGIYRISFNTGKYCPA----GFF 302
                +     W +  +  T+ DGR   L+   E    G+Y++ F TGKY  A     F+
Sbjct: 62  -----DPVSSAWNILTTGITNDDGRCPGLITK-ENFIAGVYKMRFETGKYWDALGETCFY 121

Query: 303 PYVSIVFEIRESQKLEHFHVPLLFSPFSFSTYRGS 334
           PYV IVF I  +   +H+HVPLL S FS+STYRGS
Sbjct: 122 PYVEIVFTITNTS--QHYHVPLLLSRFSYSTYRGS 138

BLAST of Tan0022462 vs. ExPASy Swiss-Prot
Match: Q8Z7Q6 (5-hydroxyisourate hydrolase OS=Salmonella typhi OX=90370 GN=hiuH PE=3 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 3.4e-13
Identity = 47/124 (37.90%), Postives = 66/124 (53.23%), Query Frame = 0

Query: 214 ITTHVLDVARGSPAAGIDVRLERWIGTQPRPLFGEVDVGGWVLEGSSATDKDGRSGQLLN 273
           ++ H+LD   G PA G++V LE            +    GW    +  TD+DGR  + L 
Sbjct: 28  LSVHILDQQTGKPAPGVEVVLE------------QKKDNGWTQLNTGHTDQDGRI-KALW 87

Query: 274 MIEAINPGIYRISFNTGKYCPA----GFFPYVSIVFEIRESQKLEHFHVPLLFSPFSFST 333
             +A  PG YR+ F TG+Y  +     FFP + + F I  S+  EH+HVPLL S + +ST
Sbjct: 88  PEKAAAPGDYRVIFKTGQYFESKKLDTFFPEIPVEFHI--SKTNEHYHVPLLLSQYGYST 136

BLAST of Tan0022462 vs. ExPASy Swiss-Prot
Match: Q8ZQ52 (5-hydroxyisourate hydrolase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=hiuH PE=3 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 3.4e-13
Identity = 47/124 (37.90%), Postives = 66/124 (53.23%), Query Frame = 0

Query: 214 ITTHVLDVARGSPAAGIDVRLERWIGTQPRPLFGEVDVGGWVLEGSSATDKDGRSGQLLN 273
           ++ H+LD   G PA G++V LE            +    GW    +  TD+DGR  + L 
Sbjct: 28  LSVHILDQQTGKPAPGVEVVLE------------QKKDNGWTQLNTGHTDQDGRI-KALW 87

Query: 274 MIEAINPGIYRISFNTGKYCPA----GFFPYVSIVFEIRESQKLEHFHVPLLFSPFSFST 333
             +A  PG YR+ F TG+Y  +     FFP + + F I  S+  EH+HVPLL S + +ST
Sbjct: 88  PEKAAAPGDYRVIFKTGQYFESKKLDTFFPEIPVEFHI--SKTNEHYHVPLLLSQYGYST 136

BLAST of Tan0022462 vs. NCBI nr
Match: XP_022946227.1 (uric acid degradation bifunctional protein TTL isoform X2 [Cucurbita moschata])

HSP 1 Score: 615.9 bits (1587), Expect = 2.0e-172
Identity = 311/333 (93.39%), Postives = 319/333 (95.80%), Query Frame = 0

Query: 1   MASNYVFGEEEFQACCGSSKFAREMVSVSPFFSLEQTVAAARYIWFNQVDVNGWLEAFSA 60
           MA++Y FGEEEFQACCGSSKFAREMVSVSPFFSLEQ V AAR IWFNQVDVNGWLEAFSA
Sbjct: 1   MAASYDFGEEEFQACCGSSKFAREMVSVSPFFSLEQAVDAARRIWFNQVDVNGWLEAFSA 60

Query: 61  HPQIGQASKSSNQTSAQWSKGEQSTALATATGSSLQELAEWNIQYQQKFGFVFLICASGR 120
           HPQIGQASKSSNQTSAQWSKGEQSTALATATGSSLQELAEWNIQYQ+KFGF+FLICASGR
Sbjct: 61  HPQIGQASKSSNQTSAQWSKGEQSTALATATGSSLQELAEWNIQYQKKFGFIFLICASGR 120

Query: 121 STNEILAELKKRYSNRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPAFARK 180
           ST+EILAELKKRYSNRPIVEFEIAAQEQMKITELRLAKLFSTKQN SST+ES+PPAFARK
Sbjct: 121 STDEILAELKKRYSNRPIVEFEIAAQEQMKITELRLAKLFSTKQNASSTKESYPPAFARK 180

Query: 181 VEDRVSIIEGHLSATSEASTGKTSQVLTRTRPPITTHVLDVARGSPAAGIDVRLERWIGT 240
           VEDRVSII GHLS  S ASTGKT Q LTRTRPPITTHVLDVARGSPAAGIDVRLERWIGT
Sbjct: 181 VEDRVSIIGGHLSTASGASTGKTLQTLTRTRPPITTHVLDVARGSPAAGIDVRLERWIGT 240

Query: 241 QPRPLFGEVDVGGWVLEGSSATDKDGRSGQLLNMIEAINPGIYRISFNTGKYCPAGFFPY 300
           QPRPLFGEVDVGGW LEGSSATDKDGRSGQLL+MIEAINPGIYRISFNTGKY PAGFFPY
Sbjct: 241 QPRPLFGEVDVGGWALEGSSATDKDGRSGQLLSMIEAINPGIYRISFNTGKYFPAGFFPY 300

Query: 301 VSIVFEIRESQKLEHFHVPLLFSPFSFSTYRGS 334
           VSIVFEIRESQKLEHFHVPLL SPFSFSTYRGS
Sbjct: 301 VSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS 333

BLAST of Tan0022462 vs. NCBI nr
Match: XP_023540278.1 (uric acid degradation bifunctional protein TTL isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 613.6 bits (1581), Expect = 1.0e-171
Identity = 308/333 (92.49%), Postives = 319/333 (95.80%), Query Frame = 0

Query: 1   MASNYVFGEEEFQACCGSSKFAREMVSVSPFFSLEQTVAAARYIWFNQVDVNGWLEAFSA 60
           MA++Y FGEEEFQACCGSSKFAREMVSVSPFFSLEQ V AAR IWFNQVDVNGWLEAFSA
Sbjct: 1   MAASYDFGEEEFQACCGSSKFAREMVSVSPFFSLEQAVDAARRIWFNQVDVNGWLEAFSA 60

Query: 61  HPQIGQASKSSNQTSAQWSKGEQSTALATATGSSLQELAEWNIQYQQKFGFVFLICASGR 120
           HPQIGQASKSSNQTSAQWSKGEQSTALATATGSSLQELAEWNIQYQ+KFGF+FLICASGR
Sbjct: 61  HPQIGQASKSSNQTSAQWSKGEQSTALATATGSSLQELAEWNIQYQKKFGFIFLICASGR 120

Query: 121 STNEILAELKKRYSNRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPAFARK 180
           ST++ILAELKKRYSNRPI+EFEIAAQEQMKITELRLAKLFSTKQN SST+ES+PPAFARK
Sbjct: 121 STDKILAELKKRYSNRPIIEFEIAAQEQMKITELRLAKLFSTKQNVSSTKESYPPAFARK 180

Query: 181 VEDRVSIIEGHLSATSEASTGKTSQVLTRTRPPITTHVLDVARGSPAAGIDVRLERWIGT 240
           VEDRVSII GHLS  S ASTGKT Q LTRTRPPITTHVLDVARGSPAAGIDVRLERWIGT
Sbjct: 181 VEDRVSIIGGHLSTVSGASTGKTLQTLTRTRPPITTHVLDVARGSPAAGIDVRLERWIGT 240

Query: 241 QPRPLFGEVDVGGWVLEGSSATDKDGRSGQLLNMIEAINPGIYRISFNTGKYCPAGFFPY 300
           QPRPLFGEVDVGGW LEGSSATDKDGRSGQLL+M+EAINPGIYRISFNTGKY PAGFFPY
Sbjct: 241 QPRPLFGEVDVGGWALEGSSATDKDGRSGQLLSMVEAINPGIYRISFNTGKYFPAGFFPY 300

Query: 301 VSIVFEIRESQKLEHFHVPLLFSPFSFSTYRGS 334
           VSIVFEIRESQKLEHFHVPLL SPFSFSTYRGS
Sbjct: 301 VSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS 333

BLAST of Tan0022462 vs. NCBI nr
Match: XP_022946215.1 (uric acid degradation bifunctional protein TTL isoform X1 [Cucurbita moschata] >KAG6597227.1 Uric acid degradation bifunctional protein TTL, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028699.1 Uric acid degradation bifunctional protein TTL [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 611.3 bits (1575), Expect = 5.0e-171
Identity = 311/334 (93.11%), Postives = 319/334 (95.51%), Query Frame = 0

Query: 1   MASNYVFGEEEFQACCGSSKFAREMVSVSPFFSLEQTVAAARYIWFNQVDVNGWLEAFSA 60
           MA++Y FGEEEFQACCGSSKFAREMVSVSPFFSLEQ V AAR IWFNQVDVNGWLEAFSA
Sbjct: 1   MAASYDFGEEEFQACCGSSKFAREMVSVSPFFSLEQAVDAARRIWFNQVDVNGWLEAFSA 60

Query: 61  HPQIGQASKSSNQTSAQWSKGEQSTALATATGSSLQELAEWNIQYQQKFGFVFLICASGR 120
           HPQIGQASKSSNQTSAQWSKGEQSTALATATGSSLQELAEWNIQYQ+KFGF+FLICASGR
Sbjct: 61  HPQIGQASKSSNQTSAQWSKGEQSTALATATGSSLQELAEWNIQYQKKFGFIFLICASGR 120

Query: 121 STNEILAELKKRYSNRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPAFARK 180
           ST+EILAELKKRYSNRPIVEFEIAAQEQMKITELRLAKLFSTKQN SST+ES+PPAFARK
Sbjct: 121 STDEILAELKKRYSNRPIVEFEIAAQEQMKITELRLAKLFSTKQNASSTKESYPPAFARK 180

Query: 181 V-EDRVSIIEGHLSATSEASTGKTSQVLTRTRPPITTHVLDVARGSPAAGIDVRLERWIG 240
           V EDRVSII GHLS  S ASTGKT Q LTRTRPPITTHVLDVARGSPAAGIDVRLERWIG
Sbjct: 181 VEEDRVSIIGGHLSTASGASTGKTLQTLTRTRPPITTHVLDVARGSPAAGIDVRLERWIG 240

Query: 241 TQPRPLFGEVDVGGWVLEGSSATDKDGRSGQLLNMIEAINPGIYRISFNTGKYCPAGFFP 300
           TQPRPLFGEVDVGGW LEGSSATDKDGRSGQLL+MIEAINPGIYRISFNTGKY PAGFFP
Sbjct: 241 TQPRPLFGEVDVGGWALEGSSATDKDGRSGQLLSMIEAINPGIYRISFNTGKYFPAGFFP 300

Query: 301 YVSIVFEIRESQKLEHFHVPLLFSPFSFSTYRGS 334
           YVSIVFEIRESQKLEHFHVPLL SPFSFSTYRGS
Sbjct: 301 YVSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS 334

BLAST of Tan0022462 vs. NCBI nr
Match: XP_023540276.1 (uric acid degradation bifunctional protein TTL isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 609.0 bits (1569), Expect = 2.5e-170
Identity = 308/334 (92.22%), Postives = 319/334 (95.51%), Query Frame = 0

Query: 1   MASNYVFGEEEFQACCGSSKFAREMVSVSPFFSLEQTVAAARYIWFNQVDVNGWLEAFSA 60
           MA++Y FGEEEFQACCGSSKFAREMVSVSPFFSLEQ V AAR IWFNQVDVNGWLEAFSA
Sbjct: 1   MAASYDFGEEEFQACCGSSKFAREMVSVSPFFSLEQAVDAARRIWFNQVDVNGWLEAFSA 60

Query: 61  HPQIGQASKSSNQTSAQWSKGEQSTALATATGSSLQELAEWNIQYQQKFGFVFLICASGR 120
           HPQIGQASKSSNQTSAQWSKGEQSTALATATGSSLQELAEWNIQYQ+KFGF+FLICASGR
Sbjct: 61  HPQIGQASKSSNQTSAQWSKGEQSTALATATGSSLQELAEWNIQYQKKFGFIFLICASGR 120

Query: 121 STNEILAELKKRYSNRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPAFARK 180
           ST++ILAELKKRYSNRPI+EFEIAAQEQMKITELRLAKLFSTKQN SST+ES+PPAFARK
Sbjct: 121 STDKILAELKKRYSNRPIIEFEIAAQEQMKITELRLAKLFSTKQNVSSTKESYPPAFARK 180

Query: 181 V-EDRVSIIEGHLSATSEASTGKTSQVLTRTRPPITTHVLDVARGSPAAGIDVRLERWIG 240
           V EDRVSII GHLS  S ASTGKT Q LTRTRPPITTHVLDVARGSPAAGIDVRLERWIG
Sbjct: 181 VEEDRVSIIGGHLSTVSGASTGKTLQTLTRTRPPITTHVLDVARGSPAAGIDVRLERWIG 240

Query: 241 TQPRPLFGEVDVGGWVLEGSSATDKDGRSGQLLNMIEAINPGIYRISFNTGKYCPAGFFP 300
           TQPRPLFGEVDVGGW LEGSSATDKDGRSGQLL+M+EAINPGIYRISFNTGKY PAGFFP
Sbjct: 241 TQPRPLFGEVDVGGWALEGSSATDKDGRSGQLLSMVEAINPGIYRISFNTGKYFPAGFFP 300

Query: 301 YVSIVFEIRESQKLEHFHVPLLFSPFSFSTYRGS 334
           YVSIVFEIRESQKLEHFHVPLL SPFSFSTYRGS
Sbjct: 301 YVSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS 334

BLAST of Tan0022462 vs. NCBI nr
Match: XP_011650818.1 (uric acid degradation bifunctional protein TTL isoform X2 [Cucumis sativus])

HSP 1 Score: 604.7 bits (1558), Expect = 4.6e-169
Identity = 305/335 (91.04%), Postives = 319/335 (95.22%), Query Frame = 0

Query: 1   MASNYVFGEEEFQACCGSSKFAREMVSVSPFFSLEQTVAAARYIWFNQVDVNGWLEAFSA 60
           MASNY F EEEFQACCGSSKFA+EMVS SPFFSLEQ +AAAR+IWFNQVDV+GWLEAFSA
Sbjct: 1   MASNYDFVEEEFQACCGSSKFAKEMVSASPFFSLEQAIAAARHIWFNQVDVSGWLEAFSA 60

Query: 61  HPQI-GQASKSSNQTSAQWSKGEQSTALATATGSSLQELAEWNIQYQQKFGFVFLICASG 120
           HP+I GQ SKSSNQTSAQWSKGEQSTA+ATATGSSLQELAEWNIQYQQKFGFVFLICASG
Sbjct: 61  HPRIGGQVSKSSNQTSAQWSKGEQSTAMATATGSSLQELAEWNIQYQQKFGFVFLICASG 120

Query: 121 RSTNEILAELKKRYSNRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPA-FA 180
           RST+EILAELKKRY +RPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPP  FA
Sbjct: 121 RSTSEILAELKKRYPSRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPPDFA 180

Query: 181 RKVEDRVSIIEGHLSATSEASTGKTSQVLTRTRPPITTHVLDVARGSPAAGIDVRLERWI 240
           RKVEDRV++I GHL ATSEASTGKTSQ+LTRTRPPITTHVLDVARGSPA GIDVRLERWI
Sbjct: 181 RKVEDRVNVIGGHLGATSEASTGKTSQILTRTRPPITTHVLDVARGSPATGIDVRLERWI 240

Query: 241 GTQPRPLFGEVDVGGWVLEGSSATDKDGRSGQLLNMIEAINPGIYRISFNTGKYCPAGFF 300
           GTQPRPLFGE DVGGW LEG+SATDKDGRSGQLL+M+EAINPGIYRISFNTGKY PAGFF
Sbjct: 241 GTQPRPLFGEADVGGWALEGTSATDKDGRSGQLLSMVEAINPGIYRISFNTGKYFPAGFF 300

Query: 301 PYVSIVFEIRESQKLEHFHVPLLFSPFSFSTYRGS 334
           PYVSIVFEIRESQKLEHFHVPLL SPFSFSTYRGS
Sbjct: 301 PYVSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS 335

BLAST of Tan0022462 vs. ExPASy TrEMBL
Match: A0A6J1G360 (Hydroxyisourate hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111450364 PE=4 SV=1)

HSP 1 Score: 615.9 bits (1587), Expect = 9.7e-173
Identity = 311/333 (93.39%), Postives = 319/333 (95.80%), Query Frame = 0

Query: 1   MASNYVFGEEEFQACCGSSKFAREMVSVSPFFSLEQTVAAARYIWFNQVDVNGWLEAFSA 60
           MA++Y FGEEEFQACCGSSKFAREMVSVSPFFSLEQ V AAR IWFNQVDVNGWLEAFSA
Sbjct: 1   MAASYDFGEEEFQACCGSSKFAREMVSVSPFFSLEQAVDAARRIWFNQVDVNGWLEAFSA 60

Query: 61  HPQIGQASKSSNQTSAQWSKGEQSTALATATGSSLQELAEWNIQYQQKFGFVFLICASGR 120
           HPQIGQASKSSNQTSAQWSKGEQSTALATATGSSLQELAEWNIQYQ+KFGF+FLICASGR
Sbjct: 61  HPQIGQASKSSNQTSAQWSKGEQSTALATATGSSLQELAEWNIQYQKKFGFIFLICASGR 120

Query: 121 STNEILAELKKRYSNRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPAFARK 180
           ST+EILAELKKRYSNRPIVEFEIAAQEQMKITELRLAKLFSTKQN SST+ES+PPAFARK
Sbjct: 121 STDEILAELKKRYSNRPIVEFEIAAQEQMKITELRLAKLFSTKQNASSTKESYPPAFARK 180

Query: 181 VEDRVSIIEGHLSATSEASTGKTSQVLTRTRPPITTHVLDVARGSPAAGIDVRLERWIGT 240
           VEDRVSII GHLS  S ASTGKT Q LTRTRPPITTHVLDVARGSPAAGIDVRLERWIGT
Sbjct: 181 VEDRVSIIGGHLSTASGASTGKTLQTLTRTRPPITTHVLDVARGSPAAGIDVRLERWIGT 240

Query: 241 QPRPLFGEVDVGGWVLEGSSATDKDGRSGQLLNMIEAINPGIYRISFNTGKYCPAGFFPY 300
           QPRPLFGEVDVGGW LEGSSATDKDGRSGQLL+MIEAINPGIYRISFNTGKY PAGFFPY
Sbjct: 241 QPRPLFGEVDVGGWALEGSSATDKDGRSGQLLSMIEAINPGIYRISFNTGKYFPAGFFPY 300

Query: 301 VSIVFEIRESQKLEHFHVPLLFSPFSFSTYRGS 334
           VSIVFEIRESQKLEHFHVPLL SPFSFSTYRGS
Sbjct: 301 VSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS 333

BLAST of Tan0022462 vs. ExPASy TrEMBL
Match: A0A6J1G375 (Hydroxyisourate hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111450364 PE=4 SV=1)

HSP 1 Score: 611.3 bits (1575), Expect = 2.4e-171
Identity = 311/334 (93.11%), Postives = 319/334 (95.51%), Query Frame = 0

Query: 1   MASNYVFGEEEFQACCGSSKFAREMVSVSPFFSLEQTVAAARYIWFNQVDVNGWLEAFSA 60
           MA++Y FGEEEFQACCGSSKFAREMVSVSPFFSLEQ V AAR IWFNQVDVNGWLEAFSA
Sbjct: 1   MAASYDFGEEEFQACCGSSKFAREMVSVSPFFSLEQAVDAARRIWFNQVDVNGWLEAFSA 60

Query: 61  HPQIGQASKSSNQTSAQWSKGEQSTALATATGSSLQELAEWNIQYQQKFGFVFLICASGR 120
           HPQIGQASKSSNQTSAQWSKGEQSTALATATGSSLQELAEWNIQYQ+KFGF+FLICASGR
Sbjct: 61  HPQIGQASKSSNQTSAQWSKGEQSTALATATGSSLQELAEWNIQYQKKFGFIFLICASGR 120

Query: 121 STNEILAELKKRYSNRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPAFARK 180
           ST+EILAELKKRYSNRPIVEFEIAAQEQMKITELRLAKLFSTKQN SST+ES+PPAFARK
Sbjct: 121 STDEILAELKKRYSNRPIVEFEIAAQEQMKITELRLAKLFSTKQNASSTKESYPPAFARK 180

Query: 181 V-EDRVSIIEGHLSATSEASTGKTSQVLTRTRPPITTHVLDVARGSPAAGIDVRLERWIG 240
           V EDRVSII GHLS  S ASTGKT Q LTRTRPPITTHVLDVARGSPAAGIDVRLERWIG
Sbjct: 181 VEEDRVSIIGGHLSTASGASTGKTLQTLTRTRPPITTHVLDVARGSPAAGIDVRLERWIG 240

Query: 241 TQPRPLFGEVDVGGWVLEGSSATDKDGRSGQLLNMIEAINPGIYRISFNTGKYCPAGFFP 300
           TQPRPLFGEVDVGGW LEGSSATDKDGRSGQLL+MIEAINPGIYRISFNTGKY PAGFFP
Sbjct: 241 TQPRPLFGEVDVGGWALEGSSATDKDGRSGQLLSMIEAINPGIYRISFNTGKYFPAGFFP 300

Query: 301 YVSIVFEIRESQKLEHFHVPLLFSPFSFSTYRGS 334
           YVSIVFEIRESQKLEHFHVPLL SPFSFSTYRGS
Sbjct: 301 YVSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS 334

BLAST of Tan0022462 vs. ExPASy TrEMBL
Match: A0A1S3AVJ3 (Hydroxyisourate hydrolase OS=Cucumis melo OX=3656 GN=LOC103483398 PE=4 SV=1)

HSP 1 Score: 604.4 bits (1557), Expect = 2.9e-169
Identity = 306/335 (91.34%), Postives = 319/335 (95.22%), Query Frame = 0

Query: 1   MASNYVFGEEEFQACCGSSKFAREMVSVSPFFSLEQTVAAARYIWFNQVDVNGWLEAFSA 60
           MASNY FGEEEFQACCGSSKFA+EMVS SPF SLEQ +AAAR+IWFNQVDV+GWLEAFSA
Sbjct: 1   MASNYDFGEEEFQACCGSSKFAKEMVSASPFSSLEQAIAAARHIWFNQVDVSGWLEAFSA 60

Query: 61  HPQI-GQASKSSNQTSAQWSKGEQSTALATATGSSLQELAEWNIQYQQKFGFVFLICASG 120
           HP+I GQ SKSSNQTSAQWSKGEQSTA+ATATGSSLQELAEWNIQYQQKFGFVFLICASG
Sbjct: 61  HPRIGGQVSKSSNQTSAQWSKGEQSTAMATATGSSLQELAEWNIQYQQKFGFVFLICASG 120

Query: 121 RSTNEILAELKKRYSNRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPA-FA 180
           RST+EILAELKKRY NRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPP  FA
Sbjct: 121 RSTSEILAELKKRYPNRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPPDFA 180

Query: 181 RKVEDRVSIIEGHLSATSEASTGKTSQVLTRTRPPITTHVLDVARGSPAAGIDVRLERWI 240
           RKVEDRV++I GHL ATSEASTGKTSQ+LTRTRPPITTHVLDVARGSPAAGIDV LERWI
Sbjct: 181 RKVEDRVNVIGGHLGATSEASTGKTSQILTRTRPPITTHVLDVARGSPAAGIDVCLERWI 240

Query: 241 GTQPRPLFGEVDVGGWVLEGSSATDKDGRSGQLLNMIEAINPGIYRISFNTGKYCPAGFF 300
           GTQPRPLFGE DVGGW LEG+SATDKDGRSGQLL+M+EAINPGIYRISFNTGKY PAGFF
Sbjct: 241 GTQPRPLFGEGDVGGWALEGTSATDKDGRSGQLLSMVEAINPGIYRISFNTGKYFPAGFF 300

Query: 301 PYVSIVFEIRESQKLEHFHVPLLFSPFSFSTYRGS 334
           PYVSIVFEIRESQKLEHFHVPLL SPFSFSTYRGS
Sbjct: 301 PYVSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS 335

BLAST of Tan0022462 vs. ExPASy TrEMBL
Match: A0A0A0L3W8 (Hydroxyisourate hydrolase OS=Cucumis sativus OX=3659 GN=Csa_3G127080 PE=4 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 5.5e-168
Identity = 305/336 (90.77%), Postives = 319/336 (94.94%), Query Frame = 0

Query: 1   MASNYVFGEEEFQACCGSSKFAREMVSVSPFFSLEQTVAAARYIWFNQVDVNGWLEAFSA 60
           MASNY F EEEFQACCGSSKFA+EMVS SPFFSLEQ +AAAR+IWFNQVDV+GWLEAFSA
Sbjct: 1   MASNYDFVEEEFQACCGSSKFAKEMVSASPFFSLEQAIAAARHIWFNQVDVSGWLEAFSA 60

Query: 61  HPQI-GQASKSSNQTSAQWSKGEQSTALATATGSSLQELAEWNIQYQQKFGFVFLICASG 120
           HP+I GQ SKSSNQTSAQWSKGEQSTA+ATATGSSLQELAEWNIQYQQKFGFVFLICASG
Sbjct: 61  HPRIGGQVSKSSNQTSAQWSKGEQSTAMATATGSSLQELAEWNIQYQQKFGFVFLICASG 120

Query: 121 RSTNEILAELKKRYSNRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPA-FA 180
           RST+EILAELKKRY +RPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPP  FA
Sbjct: 121 RSTSEILAELKKRYPSRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPPDFA 180

Query: 181 RKV-EDRVSIIEGHLSATSEASTGKTSQVLTRTRPPITTHVLDVARGSPAAGIDVRLERW 240
           RKV EDRV++I GHL ATSEASTGKTSQ+LTRTRPPITTHVLDVARGSPA GIDVRLERW
Sbjct: 181 RKVEEDRVNVIGGHLGATSEASTGKTSQILTRTRPPITTHVLDVARGSPATGIDVRLERW 240

Query: 241 IGTQPRPLFGEVDVGGWVLEGSSATDKDGRSGQLLNMIEAINPGIYRISFNTGKYCPAGF 300
           IGTQPRPLFGE DVGGW LEG+SATDKDGRSGQLL+M+EAINPGIYRISFNTGKY PAGF
Sbjct: 241 IGTQPRPLFGEADVGGWALEGTSATDKDGRSGQLLSMVEAINPGIYRISFNTGKYFPAGF 300

Query: 301 FPYVSIVFEIRESQKLEHFHVPLLFSPFSFSTYRGS 334
           FPYVSIVFEIRESQKLEHFHVPLL SPFSFSTYRGS
Sbjct: 301 FPYVSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS 336

BLAST of Tan0022462 vs. ExPASy TrEMBL
Match: A0A1S3AWH8 (Hydroxyisourate hydrolase OS=Cucumis melo OX=3656 GN=LOC103483398 PE=4 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 7.2e-168
Identity = 306/336 (91.07%), Postives = 319/336 (94.94%), Query Frame = 0

Query: 1   MASNYVFGEEEFQACCGSSKFAREMVSVSPFFSLEQTVAAARYIWFNQVDVNGWLEAFSA 60
           MASNY FGEEEFQACCGSSKFA+EMVS SPF SLEQ +AAAR+IWFNQVDV+GWLEAFSA
Sbjct: 1   MASNYDFGEEEFQACCGSSKFAKEMVSASPFSSLEQAIAAARHIWFNQVDVSGWLEAFSA 60

Query: 61  HPQI-GQASKSSNQTSAQWSKGEQSTALATATGSSLQELAEWNIQYQQKFGFVFLICASG 120
           HP+I GQ SKSSNQTSAQWSKGEQSTA+ATATGSSLQELAEWNIQYQQKFGFVFLICASG
Sbjct: 61  HPRIGGQVSKSSNQTSAQWSKGEQSTAMATATGSSLQELAEWNIQYQQKFGFVFLICASG 120

Query: 121 RSTNEILAELKKRYSNRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPA-FA 180
           RST+EILAELKKRY NRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPP  FA
Sbjct: 121 RSTSEILAELKKRYPNRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPPDFA 180

Query: 181 RKV-EDRVSIIEGHLSATSEASTGKTSQVLTRTRPPITTHVLDVARGSPAAGIDVRLERW 240
           RKV EDRV++I GHL ATSEASTGKTSQ+LTRTRPPITTHVLDVARGSPAAGIDV LERW
Sbjct: 181 RKVEEDRVNVIGGHLGATSEASTGKTSQILTRTRPPITTHVLDVARGSPAAGIDVCLERW 240

Query: 241 IGTQPRPLFGEVDVGGWVLEGSSATDKDGRSGQLLNMIEAINPGIYRISFNTGKYCPAGF 300
           IGTQPRPLFGE DVGGW LEG+SATDKDGRSGQLL+M+EAINPGIYRISFNTGKY PAGF
Sbjct: 241 IGTQPRPLFGEGDVGGWALEGTSATDKDGRSGQLLSMVEAINPGIYRISFNTGKYFPAGF 300

Query: 301 FPYVSIVFEIRESQKLEHFHVPLLFSPFSFSTYRGS 334
           FPYVSIVFEIRESQKLEHFHVPLL SPFSFSTYRGS
Sbjct: 301 FPYVSIVFEIRESQKLEHFHVPLLLSPFSFSTYRGS 336

BLAST of Tan0022462 vs. TAIR 10
Match: AT5G58220.1 (transthyretin-like protein )

HSP 1 Score: 371.7 bits (953), Expect = 6.2e-103
Identity = 194/327 (59.33%), Postives = 240/327 (73.39%), Query Frame = 0

Query: 8   GEEEFQACCGSSKFAREMVSVSPFFSLEQTVAAARYIWFNQVDVNGWLEAFSAHPQIGQA 67
           GE+E++ CCGSS+FA++M +  P  S ++ +  AR IWFNQV+V  WLEAFSAHPQIG  
Sbjct: 6   GEDEWKVCCGSSEFAKQMSTSGPLTS-QEAIYTARDIWFNQVNVTDWLEAFSAHPQIGNT 65

Query: 68  -SKSSNQTSAQWSKGEQSTALATATGSSLQELAEWNIQYQQKFGFVFLICASGRSTNEIL 127
            S S N   A+ S  EQSTA AT + S+LQELAEWN+ Y++KFGF+F+ICASGR+  E+L
Sbjct: 66  PSPSINSDFARRSVSEQSTAFATTSASALQELAEWNVLYKKKFGFIFIICASGRTHAEML 125

Query: 128 AELKKRYSNRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPAFARKVEDRVS 187
             LK+RY NRPIVE EIAA EQMKITELR+AKLFS K    S  +S     + K +DR+ 
Sbjct: 126 HALKERYENRPIVELEIAAMEQMKITELRMAKLFSDKAKVISETDSSSSPVSTKPQDRLR 185

Query: 188 IIEGHLSATSEASTGKTSQVLTRTRPPITTHVLDVARGSPAAGIDVRLERWIGTQPRPLF 247
           II GHL+  +EA   K      R+RPPITTHVLDV+RG+PAAG++V LE W GT   P F
Sbjct: 186 IIGGHLNVAAEAKAPK------RSRPPITTHVLDVSRGAPAAGVEVHLEVWSGT-TGPSF 245

Query: 248 GEVDVGGWVLEGSSATDKDGRSGQLLNMIEAINPGIYRISFNTGKYCPAGFFPYVSIVFE 307
                G W   G+SATD+DGRSG L+++++A+NPG YRISF+T KY P  FFPYVSIVF+
Sbjct: 246 VHGGGGVWSSVGTSATDRDGRSGPLMDLVDALNPGTYRISFDTAKYSPGCFFPYVSIVFQ 305

Query: 308 IRESQKLEHFHVPLLFSPFSFSTYRGS 334
           + ESQK EHFHVPLL +PFSFSTYRGS
Sbjct: 306 VTESQKWEHFHVPLLLAPFSFSTYRGS 324

BLAST of Tan0022462 vs. TAIR 10
Match: AT5G58220.3 (transthyretin-like protein )

HSP 1 Score: 348.6 bits (893), Expect = 5.6e-96
Identity = 187/327 (57.19%), Postives = 231/327 (70.64%), Query Frame = 0

Query: 8   GEEEFQACCGSSKFAREMVSVSPFFSLEQTVAAARYIWFNQVDVNGWLEAFSAHPQIGQA 67
           GE+E++ CCGSS+FA++M +  P  S ++ +  AR IWFNQV+V  WLEAFSAHPQIG  
Sbjct: 6   GEDEWKVCCGSSEFAKQMSTSGPLTS-QEAIYTARDIWFNQVNVTDWLEAFSAHPQIGNT 65

Query: 68  -SKSSNQTSAQWSKGEQSTALATATGSSLQELAEWNIQYQQKFGFVFLICASGRSTNEIL 127
            S S N   A+ S  EQSTA AT + S+LQELAEWN+ Y++KFGF+F+ICASGR+  E+L
Sbjct: 66  PSPSINSDFARRSVSEQSTAFATTSASALQELAEWNVLYKKKFGFIFIICASGRTHAEML 125

Query: 128 AELKKRYSNRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPAFARKVEDRVS 187
             LK+RY NRPIVE EIAA EQMKITELR+AKLFS K    S  +S     + K +    
Sbjct: 126 HALKERYENRPIVELEIAAMEQMKITELRMAKLFSDKAKVISETDSSSSPVSTKPQ---- 185

Query: 188 IIEGHLSATSEASTGKTSQVLTRTRPPITTHVLDVARGSPAAGIDVRLERWIGTQPRPLF 247
                    +EA   K      R+RPPITTHVLDV+RG+PAAG++V LE W GT   P F
Sbjct: 186 ---------AEAKAPK------RSRPPITTHVLDVSRGAPAAGVEVHLEVWSGT-TGPSF 245

Query: 248 GEVDVGGWVLEGSSATDKDGRSGQLLNMIEAINPGIYRISFNTGKYCPAGFFPYVSIVFE 307
                G W   G+SATD+DGRSG L+++++A+NPG YRISF+T KY P  FFPYVSIVF+
Sbjct: 246 VHGGGGVWSSVGTSATDRDGRSGPLMDLVDALNPGTYRISFDTAKYSPGCFFPYVSIVFQ 305

Query: 308 IRESQKLEHFHVPLLFSPFSFSTYRGS 334
           + ESQK EHFHVPLL +PFSFSTYRGS
Sbjct: 306 VTESQKWEHFHVPLLLAPFSFSTYRGS 311

BLAST of Tan0022462 vs. TAIR 10
Match: AT5G58220.2 (transthyretin-like protein )

HSP 1 Score: 307.4 bits (786), Expect = 1.4e-83
Identity = 172/327 (52.60%), Postives = 214/327 (65.44%), Query Frame = 0

Query: 8   GEEEFQACCGSSKFAREMVSVSPFFSLEQTVAAARYIWFNQVDVNGWLEAFSAHPQIGQA 67
           GE+E++ CCGSS+FA++M +  P  S ++ +  AR IWFNQV+V  WLEAFSAHPQIG  
Sbjct: 6   GEDEWKVCCGSSEFAKQMSTSGPLTS-QEAIYTARDIWFNQVNVTDWLEAFSAHPQIGNT 65

Query: 68  -SKSSNQTSAQWSKGEQSTALATATGSSLQELAEWNIQYQQKFGFVFLICASGRSTNEIL 127
            S S N   A+ S  EQSTA AT + S+LQELAEWN+ Y++KFGF+F+ICASGR+  E+L
Sbjct: 66  PSPSINSDFARRSVSEQSTAFATTSASALQELAEWNVLYKKKFGFIFIICASGRTHAEML 125

Query: 128 AELKKRYSNRPIVEFEIAAQEQMKITELRLAKLFSTKQNTSSTRESHPPAFARKVEDRVS 187
             LK+RY NRPIVE EIAA EQMKITELR+AKLFS                     D+  
Sbjct: 126 HALKERYENRPIVELEIAAMEQMKITELRMAKLFS---------------------DKAK 185

Query: 188 IIEGHLSATSEASTGKTSQVLTRTRPPITTHVLDVARGSPAAGIDVRLERWIGTQPRPLF 247
           +I    S++S  S          T+P              AAG++V LE W GT   P F
Sbjct: 186 VISETDSSSSPVS----------TKP-------------QAAGVEVHLEVWSGT-TGPSF 245

Query: 248 GEVDVGGWVLEGSSATDKDGRSGQLLNMIEAINPGIYRISFNTGKYCPAGFFPYVSIVFE 307
                G W   G+SATD+DGRSG L+++++A+NPG YRISF+T KY P  FFPYVSIVF+
Sbjct: 246 VHGGGGVWSSVGTSATDRDGRSGPLMDLVDALNPGTYRISFDTAKYSPGCFFPYVSIVFQ 286

Query: 308 IRESQKLEHFHVPLLFSPFSFSTYRGS 334
           + ESQK EHFHVPLL +PFSFSTYRGS
Sbjct: 306 VTESQKWEHFHVPLLLAPFSFSTYRGS 286

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LVM58.7e-10259.33Uric acid degradation bifunctional protein TTL OS=Arabidopsis thaliana OX=3702 G... [more]
Q9CRB31.5e-1643.655-hydroxyisourate hydrolase OS=Mus musculus OX=10090 GN=Urah PE=1 SV=1[more]
Q06S872.8e-1535.485-hydroxyisourate hydrolase OS=Danio rerio OX=7955 GN=urah PE=1 SV=1[more]
Q8Z7Q63.4e-1337.905-hydroxyisourate hydrolase OS=Salmonella typhi OX=90370 GN=hiuH PE=3 SV=1[more]
Q8ZQ523.4e-1337.905-hydroxyisourate hydrolase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / A... [more]
Match NameE-valueIdentityDescription
XP_022946227.12.0e-17293.39uric acid degradation bifunctional protein TTL isoform X2 [Cucurbita moschata][more]
XP_023540278.11.0e-17192.49uric acid degradation bifunctional protein TTL isoform X2 [Cucurbita pepo subsp.... [more]
XP_022946215.15.0e-17193.11uric acid degradation bifunctional protein TTL isoform X1 [Cucurbita moschata] >... [more]
XP_023540276.12.5e-17092.22uric acid degradation bifunctional protein TTL isoform X1 [Cucurbita pepo subsp.... [more]
XP_011650818.14.6e-16991.04uric acid degradation bifunctional protein TTL isoform X2 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
A0A6J1G3609.7e-17393.39Hydroxyisourate hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111450364 PE=4 SV=... [more]
A0A6J1G3752.4e-17193.11Hydroxyisourate hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111450364 PE=4 SV=... [more]
A0A1S3AVJ32.9e-16991.34Hydroxyisourate hydrolase OS=Cucumis melo OX=3656 GN=LOC103483398 PE=4 SV=1[more]
A0A0A0L3W85.5e-16890.77Hydroxyisourate hydrolase OS=Cucumis sativus OX=3659 GN=Csa_3G127080 PE=4 SV=1[more]
A0A1S3AWH87.2e-16891.07Hydroxyisourate hydrolase OS=Cucumis melo OX=3656 GN=LOC103483398 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G58220.16.2e-10359.33transthyretin-like protein [more]
AT5G58220.35.6e-9657.19transthyretin-like protein [more]
AT5G58220.21.4e-8352.60transthyretin-like protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000895Transthyretin/hydroxyisourate hydrolasePRINTSPR00189TRNSTHYRETINcoord: 213..233
score: 46.26
coord: 311..333
score: 35.2
IPR023416Transthyretin/hydroxyisourate hydrolase domainPFAMPF00576Transthyretincoord: 214..332
e-value: 7.4E-31
score: 107.1
IPR023416Transthyretin/hydroxyisourate hydrolase domainPANTHERPTHR10395URICASE AND TRANSTHYRETIN-RELATEDcoord: 7..333
IPR036817Transthyretin/hydroxyisourate hydrolase domain superfamilyGENE3D2.60.40.180Transthyretin/hydroxyisourate hydrolase domaincoord: 203..331
e-value: 1.5E-37
score: 130.2
IPR036817Transthyretin/hydroxyisourate hydrolase domain superfamilySUPERFAMILY49472Transthyretin (synonym: prealbumin)coord: 210..333
IPR036778Oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase superfamilyGENE3D1.10.3330.10coord: 8..163
e-value: 1.1E-51
score: 177.3
IPR036778Oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase superfamilySUPERFAMILY158694UraD-Likecoord: 13..161
IPR018020Oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylasePFAMPF09349OHCU_decarboxcoord: 10..157
e-value: 3.4E-30
score: 105.6
IPR014306Hydroxyisourate hydrolaseTIGRFAMTIGR02962TIGR02962coord: 213..333
e-value: 8.8E-27
score: 91.7
IPR014306Hydroxyisourate hydrolaseCDDcd05822TLP_HIUasecoord: 212..333
e-value: 8.85479E-47
score: 152.309
NoneNo IPR availablePANTHERPTHR10395:SF75-HYDROXYISOURATE HYDROLASEcoord: 7..333
IPR023418Transthyretin, thyroxine binding sitePROSITEPS00768TRANSTHYRETIN_1coord: 217..232

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0022462.1Tan0022462.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019428 allantoin biosynthetic process
biological_process GO:0009742 brassinosteroid mediated signaling pathway
biological_process GO:0006144 purine nucleobase metabolic process
biological_process GO:0001560 regulation of cell growth by extracellular stimulus
cellular_component GO:0005829 cytosol
cellular_component GO:0031234 extrinsic component of cytoplasmic side of plasma membrane
cellular_component GO:0005777 peroxisome
molecular_function GO:0051997 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase activity
molecular_function GO:0033971 hydroxyisourate hydrolase activity
molecular_function GO:0042802 identical protein binding