Tan0018511 (gene) Snake gourd v1

Overview
NameTan0018511
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptiont-SNARE coiled-coil homology domain-containing protein
LocationLG04: 9079210 .. 9080936 (+)
RNA-Seq ExpressionTan0018511
SyntenyTan0018511
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTAGTTTCATGAAATCCCCCGCGAAAGTTTCTAAGCCGAATTCTGAAGATCCTGGATTATCAGCCGGATCTGGCACTAATCCTTTTGATTCAGATACTGGACTTGAAACCAACCAAACTCCTAATCCTGCAAGAAGAACTTCTTCCGAGCCTGCACTCGTCATACCGAAATCCAATTCCCCCAATCCTTTTGATGATGATGATGATGGTTTTGTTGGAAGAAGAGGTACTGCAACTTCTAAACCCTCTAAAGACAGGTACAAAAATGATTTTCGCGACTCGGGAGGGCTTGAGAACCAGAGTGTGCAGGAATTGGAGAACTACGCCGTGTATAAAGCTGAGGAGACCACAAAATCTGTGAATAACTGCCTGAAGATCGCTGAGGACATAAGGGGAGATGCTACCAGGACCCTTGACATGTTGCATCAGCAGGGTGAGCAAATTGAGAGGACCCACAGGATGGCTGCTGATATGGATAAAGATCTAAGTAAGGTAGGCTCTTTACTAGTTTCCATCAGACATCTTTCGGTTTTATATTTTCTACCCTATGTTGTTATCCTGAAATGATTAGTTTGCCCATGTTGATTTGATATAGTTAAAAAAAGAATCAAATAATTCAAAATTTGAAAGTGGGCTGTTATGAAATACTTGGCTTGCAAGCTTTTGTGATTAGGTTGTGGATGTCCACTCCTACTCTGTAAGAGATGGAAAATGGTCAAGAACAAATAACCTTTTAGTTTGCAAGGAATCTGATATGCTAGCAAGAAAATGAATGAAGTTATTTTATCTTGCTTTTGCTTTGCTTAATGTATGTGTTCTCTGGGTTCGTTAATCGTTTTTCCTATTCATTTTTATTAATTATAGGGAGAGAAACTTCTGAATAATCTTGGAGGCATGTTTTCTAAGCCATGGAAGCCAAAGAAGACCAGAGAAATTACAGGCCCTCTTATAACAGCAGGTTACATTTTCACTGGTTCTTTGGCTCACTAAATACTGTCATTCCTATAACTCACTGCCACTCCCATTTTATCTGCTATTGATTATTTCCATTTTGGCTGTCCAGACAATTCCTCCGGGAGAGCTGATAACAACAAGGAGGAACGGGAAAAACTGGGTGTATCTACGGGCAAAAAGCAGTCGGCTTCTCGAACACCACCTTCTGAATCATCAGGCGCAATGCAAAAAGTTGAGGTGAGAGTATCGGCTGTTATCATTCCACTATCTTAAGAGTATAGTATATATTATTATGGTTTCAGATCTAACCAATGTGTAAGAATATTGTTGTTTCAGGTTGAGAAGGAAAAGCAAGATGATGCACTCTCAGATTTAAGTAATATCTTAGGTGATCTGAAGAACATGGCTGTGGACATGGGAAGCGAGCTTGACAGGTCATATCTGCCATCCTCTTTTCTCAACTTAGACATTGTTGTATTTATTATCTCATCAATCTGTTGCCGTACTCTACATGGTTCTGAATGCAAATTTTCTGGAAAATGCAGGCAAAACAAAGCTCTGGATCATCTAAGTGACGATGTCGACGAGCTGAATTCTAGAGTGAAAGGCGCGAATCAACGAGCTCGCCGTTTGGTTGGGTAGTAAAATCAGACACTTTTATTCTTTTTCCCACCTCTCACTCCCTTCTGTTTGTTTCTAGCTGGAAGCATTTCGTTTAAACATTCTTTAATTGTTGGTGTACTTCTATTCTTTTCTCTTGTGGTTTTAAG

mRNA sequence

ATGTTTAGTTTCATGAAATCCCCCGCGAAAGTTTCTAAGCCGAATTCTGAAGATCCTGGATTATCAGCCGGATCTGGCACTAATCCTTTTGATTCAGATACTGGACTTGAAACCAACCAAACTCCTAATCCTGCAAGAAGAACTTCTTCCGAGCCTGCACTCGTCATACCGAAATCCAATTCCCCCAATCCTTTTGATGATGATGATGATGGTTTTGTTGGAAGAAGAGGTACTGCAACTTCTAAACCCTCTAAAGACAGGTACAAAAATGATTTTCGCGACTCGGGAGGGCTTGAGAACCAGAGTGTGCAGGAATTGGAGAACTACGCCGTGTATAAAGCTGAGGAGACCACAAAATCTGTGAATAACTGCCTGAAGATCGCTGAGGACATAAGGGGAGATGCTACCAGGACCCTTGACATGTTGCATCAGCAGGGTGAGCAAATTGAGAGGACCCACAGGATGGCTGCTGATATGGATAAAGATCTAAGTAAGGGAGAGAAACTTCTGAATAATCTTGGAGGCATGTTTTCTAAGCCATGGAAGCCAAAGAAGACCAGAGAAATTACAGGCCCTCTTATAACAGCAGACAATTCCTCCGGGAGAGCTGATAACAACAAGGAGGAACGGGAAAAACTGGGTGTATCTACGGGCAAAAAGCAGTCGGCTTCTCGAACACCACCTTCTGAATCATCAGGCGCAATGCAAAAAGTTGAGGTTGAGAAGGAAAAGCAAGATGATGCACTCTCAGATTTAAGTAATATCTTAGGTGATCTGAAGAACATGGCTGTGGACATGGGAAGCGAGCTTGACAGGCAAAACAAAGCTCTGGATCATCTAAGTGACGATGTCGACGAGCTGAATTCTAGAGTGAAAGGCGCGAATCAACGAGCTCGCCGTTTGGTTGGGTAGTAAAATCAGACACTTTTATTCTTTTTCCCACCTCTCACTCCCTTCTGTTTGTTTCTAGCTGGAAGCATTTCGTTTAAACATTCTTTAATTGTTGGTGTACTTCTATTCTTTTCTCTTGTGGTTTTAAG

Coding sequence (CDS)

ATGTTTAGTTTCATGAAATCCCCCGCGAAAGTTTCTAAGCCGAATTCTGAAGATCCTGGATTATCAGCCGGATCTGGCACTAATCCTTTTGATTCAGATACTGGACTTGAAACCAACCAAACTCCTAATCCTGCAAGAAGAACTTCTTCCGAGCCTGCACTCGTCATACCGAAATCCAATTCCCCCAATCCTTTTGATGATGATGATGATGGTTTTGTTGGAAGAAGAGGTACTGCAACTTCTAAACCCTCTAAAGACAGGTACAAAAATGATTTTCGCGACTCGGGAGGGCTTGAGAACCAGAGTGTGCAGGAATTGGAGAACTACGCCGTGTATAAAGCTGAGGAGACCACAAAATCTGTGAATAACTGCCTGAAGATCGCTGAGGACATAAGGGGAGATGCTACCAGGACCCTTGACATGTTGCATCAGCAGGGTGAGCAAATTGAGAGGACCCACAGGATGGCTGCTGATATGGATAAAGATCTAAGTAAGGGAGAGAAACTTCTGAATAATCTTGGAGGCATGTTTTCTAAGCCATGGAAGCCAAAGAAGACCAGAGAAATTACAGGCCCTCTTATAACAGCAGACAATTCCTCCGGGAGAGCTGATAACAACAAGGAGGAACGGGAAAAACTGGGTGTATCTACGGGCAAAAAGCAGTCGGCTTCTCGAACACCACCTTCTGAATCATCAGGCGCAATGCAAAAAGTTGAGGTTGAGAAGGAAAAGCAAGATGATGCACTCTCAGATTTAAGTAATATCTTAGGTGATCTGAAGAACATGGCTGTGGACATGGGAAGCGAGCTTGACAGGCAAAACAAAGCTCTGGATCATCTAAGTGACGATGTCGACGAGCTGAATTCTAGAGTGAAAGGCGCGAATCAACGAGCTCGCCGTTTGGTTGGGTAG

Protein sequence

MFSFMKSPAKVSKPNSEDPGLSAGSGTNPFDSDTGLETNQTPNPARRTSSEPALVIPKSNSPNPFDDDDDGFVGRRGTATSKPSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKSVNNCLKIAEDIRGDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKPWKPKKTREITGPLITADNSSGRADNNKEEREKLGVSTGKKQSASRTPPSESSGAMQKVEVEKEKQDDALSDLSNILGDLKNMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARRLVG
Homology
BLAST of Tan0018511 vs. ExPASy Swiss-Prot
Match: Q9LMG8 (Putative SNAP25 homologous protein SNAP30 OS=Arabidopsis thaliana OX=3702 GN=SNAP30 PE=3 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 4.2e-87
Identity = 182/302 (60.26%), Postives = 212/302 (70.20%), Query Frame = 0

Query: 1   MFSFMKSPAKVSKPNSEDPGLSAGSGTNPFDSDTGLETNQTPNPARRTSSEPALVIPKSN 60
           MF F KSP                 G N   +++      T    RRTSSEP L+ P   
Sbjct: 1   MFGFFKSP-----------------GNNKLPNESSNNKGGTITAGRRTSSEPILITP--- 60

Query: 61  SPNPFDDDDDGFVGRRGTATSKPSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKS 120
               FDDD                 D+YKN F DSGGL++Q+ +ELE YAVYKAEETTK 
Sbjct: 61  ---DFDDD-----------------DKYKNGFNDSGGLQSQTTEELEKYAVYKAEETTKG 120

Query: 121 VNNCLKIAEDIRGDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP 180
           VNNCLKIAEDIR D  RTL+MLHQQGEQI RTH MA DMDKDLS+GEKLLNNLGGMFSKP
Sbjct: 121 VNNCLKIAEDIRSDGARTLEMLHQQGEQINRTHEMAVDMDKDLSRGEKLLNNLGGMFSKP 180

Query: 181 WKPKKTREITGPLITADNSSGRADNNKEEREKLGVSTGKKQSASRTPPSESSGAMQKVEV 240
           WKPKKT+ ITGP+IT D  S +++N+KEEREKLG+   K +S+S+    + + A+QKVE 
Sbjct: 181 WKPKKTKNITGPMITPDKPSKKSENHKEEREKLGLG-AKGRSSSQPALDQPTNALQKVEQ 240

Query: 241 EKEKQDDALSDLSNILGDLKNMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARR 300
           EK KQDD LSDLS+ILGDLK+MAVDMGSE+D+QNKALDHL DDVDELNSRV+GANQRAR 
Sbjct: 241 EKAKQDDGLSDLSDILGDLKSMAVDMGSEIDKQNKALDHLGDDVDELNSRVQGANQRARH 261

Query: 301 LV 303
           L+
Sbjct: 301 LL 261

BLAST of Tan0018511 vs. ExPASy Swiss-Prot
Match: Q9S7P9 (SNAP25 homologous protein SNAP33 OS=Arabidopsis thaliana OX=3702 GN=SNAP33 PE=1 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 7.2e-87
Identity = 186/309 (60.19%), Postives = 228/309 (73.79%), Query Frame = 0

Query: 1   MFSFMKSPAKVSKPNSEDPGLSAGSGTNPFDSDTGLETNQTPNPARRTSSEPALVIPKSN 60
           MF   KSPA + K NS D      S  NPFDSD   +   T NP++RT+SEP+L    ++
Sbjct: 1   MFGLRKSPANLPKHNSVD---LKSSKPNPFDSDDESDNKHTLNPSKRTTSEPSL----AD 60

Query: 61  SPNPFDDDDDGFVGRRGTATSKP-----SKDRYKNDFRDSGGLENQSVQELENYAVYKAE 120
             NPF  +    V +  +++SK      SK +YKN+FRDSGG+ENQSVQELE YAVYKAE
Sbjct: 61  MTNPFGGER---VQKGDSSSSKQSLFSNSKYQYKNNFRDSGGIENQSVQELEGYAVYKAE 120

Query: 121 ETTKSVNNCLKIAEDIRGDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGG 180
           ETTKSV  CLK+AEDIR DATRTL MLH QGEQI RTH  A ++D DLS+GEKLL +LGG
Sbjct: 121 ETTKSVQGCLKVAEDIRSDATRTLVMLHDQGEQITRTHHKAVEIDHDLSRGEKLLGSLGG 180

Query: 181 MFSKPWKPKKTREITGPLITADNSSGRADNNKEEREKLGVSTGKK-QSASRTPPSESSGA 240
           MFSK WKPKKTR I GP++T D+S  R  N+ E+REKLG+++  + QS +R P  ES+ A
Sbjct: 181 MFSKTWKPKKTRPINGPVVTRDDSPTRRVNHLEKREKLGLNSAPRGQSRTREPLPESADA 240

Query: 241 MQKVEVEKEKQDDALSDLSNILGDLKNMAVDMGSELDRQNKALDHLSDDVDELNSRVKGA 300
            Q+VE+EK KQDD LSDLS+ILG+LKNMAVDMGSE+++QNK LDHL DDVDELN RV+ +
Sbjct: 241 YQRVEMEKAKQDDGLSDLSDILGELKNMAVDMGSEIEKQNKGLDHLHDDVDELNFRVQQS 299

Query: 301 NQRARRLVG 304
           NQR RRL+G
Sbjct: 301 NQRGRRLLG 299

BLAST of Tan0018511 vs. ExPASy Swiss-Prot
Match: Q9SD96 (SNAP25 homologous protein SNAP29 OS=Arabidopsis thaliana OX=3702 GN=SNAP29 PE=2 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 9.8e-60
Identity = 131/245 (53.47%), Postives = 173/245 (70.61%), Query Frame = 0

Query: 58  KSNSPNPFDDDDDGFVGRRGTATSKPSKDRYKNDFRDSGGLENQSVQELENYAVYKAEET 117
           KS S NPFDDDDD           K  + R+ +  + SGG ENQ+VQELE+YAVY +EET
Sbjct: 19  KSFSLNPFDDDDD----------DKEVEKRFTSSLKPSGGKENQTVQELESYAVYNSEET 78

Query: 118 TKSVNNCLKIAEDIRGDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMF 177
           TK+V  CLK+AE+IR DA++TL ML++QG+QI RTH+   D+D  LS+GEK+L  LGG+F
Sbjct: 79  TKTVQGCLKVAEEIRCDASKTLVMLNEQGDQITRTHQKTVDLDHHLSRGEKILGRLGGVF 138

Query: 178 SKPWKPKKTREITGPLITADNSSGRADNNKEEREKLGVSTGKKQSASRTPPSESSGAMQK 237
           S+ WKPKK+R ITGP+IT  +S  R   + + REKLG++   K  +   P  E+  A QK
Sbjct: 139 SRTWKPKKSRSITGPVITKGDSPKRKVIDLKTREKLGLNPSLKPKSKTLP--EAVDAYQK 198

Query: 238 VEVEKEKQDDALSDLSNILGDLKNMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQR 297
            ++   KQD+AL+DLS +LG+LKNMAVDMG+ ++RQ   LDHL D+ DELN RVK +NQR
Sbjct: 199 TQI--AKQDEALTDLSALLGELKNMAVDMGTAIERQTNELDHLQDNADELNYRVKQSNQR 249

Query: 298 ARRLV 303
           AR L+
Sbjct: 259 ARYLL 249

BLAST of Tan0018511 vs. ExPASy Swiss-Prot
Match: P36977 (Synaptosomal-associated protein 25-A OS=Carassius auratus OX=7957 GN=snap25a PE=2 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 3.9e-16
Identity = 61/210 (29.05%), Postives = 110/210 (52.38%), Query Frame = 0

Query: 103 VQELENYAVYKAEETTKSVNNCLKIAEDIRGDATRTLDMLHQQGEQIERTHRMAADMDKD 162
           + +++  A   A+E+ +S    L++ E+ +    RTL ML +QGEQ+ER       ++KD
Sbjct: 11  LSDMQQRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKD 70

Query: 163 LSKGEKLLNNLG---GMFSKPWKPKKTREITGPLITADNSSGRADNNK----EEREKLGV 222
           +   EK LN+LG   G+ S P    K+    G     +N  G   +      +ERE++ +
Sbjct: 71  MKDAEKNLNDLGKFCGLCSCPCNKMKS---GGSKAWGNNQDGVVASQPARVVDEREQMAI 130

Query: 223 STGKKQSASRTPPSESSGAMQKV--EVEKEKQDDALSDLSNILGDLKNMAVDMGSELDRQ 282
                          S G +++V  +  + + D+ L  +  I+G+L++MA+DMG+E+D Q
Sbjct: 131 ---------------SGGFIRRVTDDARENEMDENLEQVGGIIGNLRHMALDMGNEIDTQ 190

Query: 283 NKALDHLSDDVDELNSRVKGANQRARRLVG 304
           N+ +D + +  D   +R+  ANQRA +++G
Sbjct: 191 NRQIDRIMEKADSNKTRIDEANQRATKMLG 202

BLAST of Tan0018511 vs. ExPASy Swiss-Prot
Match: Q17QQ3 (Synaptosomal-associated protein 25 OS=Bos taurus OX=9913 GN=SNAP25 PE=1 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 3.3e-15
Identity = 59/208 (28.37%), Postives = 109/208 (52.40%), Query Frame = 0

Query: 103 VQELENYAVYKAEETTKSVNNCLKIAEDIRGDATRTLDMLHQQGEQIERTHRMAADMDKD 162
           ++E++  A   A+E+ +S    L++ E+ +    RTL ML +QGEQ+ER       ++KD
Sbjct: 11  LEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKD 70

Query: 163 LSKGEKLLNNLG---GMFSKPWKPKKTREITGPLITADNSSGRADNNK----EEREKLGV 222
           + + EK L +LG   G+   P    K+ +        +N  G   +      +ERE++ +
Sbjct: 71  MKEAEKNLTDLGKFCGLCVCPCNKLKSSD-AYKKAWGNNQDGVVASQPARVVDEREQMAI 130

Query: 223 STGKKQSASRTPPSESSGAMQKVEVEKEKQDDALSDLSNILGDLKNMAVDMGSELDRQNK 282
           S G  +  +              +  + + D+ L  +S I+G+L++MA+DMG+E+D QN+
Sbjct: 131 SGGFIRRVTN-------------DARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNR 190

Query: 283 ALDHLSDDVDELNSRVKGANQRARRLVG 304
            +D + +  D   +R+  ANQRA +++G
Sbjct: 191 QIDRIMEKADSNKTRIDEANQRATKMLG 204

BLAST of Tan0018511 vs. NCBI nr
Match: XP_038891847.1 (putative SNAP25 homologous protein SNAP30 [Benincasa hispida] >XP_038891848.1 putative SNAP25 homologous protein SNAP30 [Benincasa hispida])

HSP 1 Score: 518.5 bits (1334), Expect = 4.0e-143
Identity = 269/303 (88.78%), Postives = 283/303 (93.40%), Query Frame = 0

Query: 1   MFSFMKSPAKVSKPNSEDPGLSAGSGTNPFDSDTGLETNQTPNPARRTSSEPALVIPKSN 60
           MF FMKSPAKV+K NS DP LS GSGTNPFDSDTG +  QT N ARRTSSEP L++P S 
Sbjct: 1   MFGFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLIMPNSM 60

Query: 61  SPNPFDDDDDGFVGRRGTATSKPSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKS 120
           S NPFDDDD+GFVGRRGTATS  SKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKS
Sbjct: 61  SSNPFDDDDEGFVGRRGTATSSASKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKS 120

Query: 121 VNNCLKIAEDIRGDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP 180
           VNNCLKIAEDIR DAT+TLDMLH+QGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP
Sbjct: 121 VNNCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP 180

Query: 181 WKPKKTREITGPLITADNSSGRADNNKEEREKLGVSTGKKQSASRTPPSESSGAMQKVEV 240
           WKPKKTREITGPLITADNSSGR +NNKE+REKLGVSTGKKQSA++TPPSE SGAMQKVE 
Sbjct: 181 WKPKKTREITGPLITADNSSGRTENNKEQREKLGVSTGKKQSATQTPPSEQSGAMQKVEA 240

Query: 241 EKEKQDDALSDLSNILGDLKNMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARR 300
           EKEKQDDALSDLSNILGDLK+MAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR+
Sbjct: 241 EKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARK 300

Query: 301 LVG 304
           L+G
Sbjct: 301 LIG 303

BLAST of Tan0018511 vs. NCBI nr
Match: XP_022945438.1 (putative SNAP25 homologous protein SNAP30 [Cucurbita moschata] >XP_022945439.1 putative SNAP25 homologous protein SNAP30 [Cucurbita moschata] >XP_022945441.1 putative SNAP25 homologous protein SNAP30 [Cucurbita moschata])

HSP 1 Score: 509.6 bits (1311), Expect = 1.8e-140
Identity = 278/317 (87.70%), Postives = 284/317 (89.59%), Query Frame = 0

Query: 1   MFSFMKSPAKVSKPNSEDPGLSAGSGTNPFDSDTGLETNQTPNPARRTSSEPALVIPKSN 60
           MFSFMKSP KVSK NS D GL AGSGTNPFDSDT  ETNQT NPARRTSSEPALVIP S 
Sbjct: 1   MFSFMKSPVKVSKQNSVDTGL-AGSGTNPFDSDTEPETNQTLNPARRTSSEPALVIPNSI 60

Query: 61  SPNPF--DDDDDGFVGRRGT------------ATSKPSKDRYKNDFRDSGGLENQSVQEL 120
           S NPF  DDDDDGFVGRRGT            ATS  SK+ YKNDFRDSGG ENQSVQEL
Sbjct: 61  SANPFDDDDDDDGFVGRRGTATSSASKNGYKNATSSASKNGYKNDFRDSGGFENQSVQEL 120

Query: 121 ENYAVYKAEETTKSVNNCLKIAEDIRGDATRTLDMLHQQGEQIERTHRMAADMDKDLSKG 180
           ENYAVYKAEETT SVNNCLKIAEDIR DATRTLDMLHQQGEQIERTHRMAADMDKDLSKG
Sbjct: 121 ENYAVYKAEETTNSVNNCLKIAEDIRSDATRTLDMLHQQGEQIERTHRMAADMDKDLSKG 180

Query: 181 EKLLNNLGGMFSKPWKPKKTREITGPLITADNSSGRADNNKEEREKLGVSTGKKQSASRT 240
           EKLLNNLGGMFSKPWKPKKT+EITGPLITADNSSGR +NNKEEREKLG+STGKKQSA+RT
Sbjct: 181 EKLLNNLGGMFSKPWKPKKTKEITGPLITADNSSGRTENNKEEREKLGLSTGKKQSATRT 240

Query: 241 PPSESSGAMQKVEVEKEKQDDALSDLSNILGDLKNMAVDMGSELDRQNKALDHLSDDVDE 300
           PPSESSGAMQKVE EKEKQDDALSDLSNILGDLKNMAVDMGSELDRQNKALDHLSDDVDE
Sbjct: 241 PPSESSGAMQKVEDEKEKQDDALSDLSNILGDLKNMAVDMGSELDRQNKALDHLSDDVDE 300

Query: 301 LNSRVKGANQRARRLVG 304
           LNSRVKGANQRARRL+G
Sbjct: 301 LNSRVKGANQRARRLLG 316

BLAST of Tan0018511 vs. NCBI nr
Match: XP_023542548.1 (putative SNAP25 homologous protein SNAP30 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 509.2 bits (1310), Expect = 2.4e-140
Identity = 278/318 (87.42%), Postives = 284/318 (89.31%), Query Frame = 0

Query: 1   MFSFMKSPAKVSKPNSEDPGLSAGSGTNPFDSDTGLETNQTPNPARRTSSEPALVIPKSN 60
           MFSFMKSP KVSK NS D GL AGSGTNPFDSDT  ETNQT NPARRTSSEPALVIP S 
Sbjct: 1   MFSFMKSPVKVSKQNSVDTGL-AGSGTNPFDSDTEPETNQTLNPARRTSSEPALVIPNSI 60

Query: 61  SPNPF---DDDDDGFVGRRGT------------ATSKPSKDRYKNDFRDSGGLENQSVQE 120
           S NPF   DDDDDGFVGRRGT            ATS  SK+ YKNDFRDSGG ENQSVQE
Sbjct: 61  SANPFDDDDDDDDGFVGRRGTATSSASKNGYKNATSSASKNGYKNDFRDSGGFENQSVQE 120

Query: 121 LENYAVYKAEETTKSVNNCLKIAEDIRGDATRTLDMLHQQGEQIERTHRMAADMDKDLSK 180
           LENYAVYKAEETT SVNNCLKIAEDIR DATRTLDMLHQQGEQIERTHRMAADMDKDLSK
Sbjct: 121 LENYAVYKAEETTNSVNNCLKIAEDIRSDATRTLDMLHQQGEQIERTHRMAADMDKDLSK 180

Query: 181 GEKLLNNLGGMFSKPWKPKKTREITGPLITADNSSGRADNNKEEREKLGVSTGKKQSASR 240
           GEKLLNNLGGMFSKPWKPKKT+EITGPLITADNSSGR +NNKEEREKLG+STGKKQSA+R
Sbjct: 181 GEKLLNNLGGMFSKPWKPKKTKEITGPLITADNSSGRTENNKEEREKLGLSTGKKQSATR 240

Query: 241 TPPSESSGAMQKVEVEKEKQDDALSDLSNILGDLKNMAVDMGSELDRQNKALDHLSDDVD 300
           TPPSESSGAMQKVE EKEKQDDALSDLSNILGDLKNMAVDMGSELDRQNKALDHLSDDVD
Sbjct: 241 TPPSESSGAMQKVEDEKEKQDDALSDLSNILGDLKNMAVDMGSELDRQNKALDHLSDDVD 300

Query: 301 ELNSRVKGANQRARRLVG 304
           ELNSRVKGANQRARRL+G
Sbjct: 301 ELNSRVKGANQRARRLLG 317

BLAST of Tan0018511 vs. NCBI nr
Match: KAG6574050.1 (SNAP25-likeous protein SNAP33, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 506.9 bits (1304), Expect = 1.2e-139
Identity = 277/319 (86.83%), Postives = 283/319 (88.71%), Query Frame = 0

Query: 1   MFSFMKSPAKVSKPNSEDPGLSAGSGTNPFDSDTGLETNQTPNPARRTSSEPALVIPKSN 60
           MFSFMKSP KVSK NS D GL AGSGTNPFDSDT  ETN T NPARRTSSEPALVIP S 
Sbjct: 1   MFSFMKSPVKVSKQNSVDTGL-AGSGTNPFDSDTEPETNHTLNPARRTSSEPALVIPNSI 60

Query: 61  SPNPF----DDDDDGFVGRRGT------------ATSKPSKDRYKNDFRDSGGLENQSVQ 120
           S NPF    DDDDDGFVGRRGT            ATS  SK+ YKNDFRDSGG ENQSVQ
Sbjct: 61  SANPFDDDDDDDDDGFVGRRGTATSSASKNGYKNATSSASKNGYKNDFRDSGGFENQSVQ 120

Query: 121 ELENYAVYKAEETTKSVNNCLKIAEDIRGDATRTLDMLHQQGEQIERTHRMAADMDKDLS 180
           ELENYAVYKAEETT SVNNCLKIAEDIR DATRTLDMLHQQGEQIERTHRMAADMDKDLS
Sbjct: 121 ELENYAVYKAEETTNSVNNCLKIAEDIRSDATRTLDMLHQQGEQIERTHRMAADMDKDLS 180

Query: 181 KGEKLLNNLGGMFSKPWKPKKTREITGPLITADNSSGRADNNKEEREKLGVSTGKKQSAS 240
           KGEKLLNNLGGMFSKPWKPKKT+EITGPLITADNSSGR +NNKEEREKLG+STGKKQSA+
Sbjct: 181 KGEKLLNNLGGMFSKPWKPKKTKEITGPLITADNSSGRTENNKEEREKLGLSTGKKQSAT 240

Query: 241 RTPPSESSGAMQKVEVEKEKQDDALSDLSNILGDLKNMAVDMGSELDRQNKALDHLSDDV 300
           RTPPSESSGAMQKVE EKEKQDDALSDLSNILGDLKNMAVDMGSELDRQNKALDHLSDDV
Sbjct: 241 RTPPSESSGAMQKVEDEKEKQDDALSDLSNILGDLKNMAVDMGSELDRQNKALDHLSDDV 300

Query: 301 DELNSRVKGANQRARRLVG 304
           DELNSRVKGANQRARRL+G
Sbjct: 301 DELNSRVKGANQRARRLLG 318

BLAST of Tan0018511 vs. NCBI nr
Match: XP_022968314.1 (putative SNAP25 homologous protein SNAP30 [Cucurbita maxima] >XP_022968315.1 putative SNAP25 homologous protein SNAP30 [Cucurbita maxima] >XP_022968317.1 putative SNAP25 homologous protein SNAP30 [Cucurbita maxima])

HSP 1 Score: 506.5 bits (1303), Expect = 1.6e-139
Identity = 276/317 (87.07%), Postives = 285/317 (89.91%), Query Frame = 0

Query: 1   MFSFMKSPAKVSKPNSEDPGLSAGSGTNPFDSDTGLETNQTPNPARRTSSEPALVIPKSN 60
           MFSFMKSPAKVSK NS DPGL AGSGTNPFDSDT  ETNQ  NPARRTSSEPALVI  S 
Sbjct: 1   MFSFMKSPAKVSKQNSVDPGL-AGSGTNPFDSDTEPETNQPLNPARRTSSEPALVILNSI 60

Query: 61  SPNPF--DDDDDGFVGRRGTATSKPSK------------DRYKNDFRDSGGLENQSVQEL 120
           SPNPF  DDDDDGFVGRRGTATS  SK            + YKNDFRDSGG E+QSVQEL
Sbjct: 61  SPNPFDDDDDDDGFVGRRGTATSSASKNGYKNATSSASINGYKNDFRDSGGFESQSVQEL 120

Query: 121 ENYAVYKAEETTKSVNNCLKIAEDIRGDATRTLDMLHQQGEQIERTHRMAADMDKDLSKG 180
           ENYAVYKAEETT SVNNCLKIAEDIR DATRTLDMLHQQGEQIERTHRMAADMDKDLSKG
Sbjct: 121 ENYAVYKAEETTNSVNNCLKIAEDIRSDATRTLDMLHQQGEQIERTHRMAADMDKDLSKG 180

Query: 181 EKLLNNLGGMFSKPWKPKKTREITGPLITADNSSGRADNNKEEREKLGVSTGKKQSASRT 240
           EKLLNNLGGMFSKPWKPKKT+EITGPLITADNSSGR +NNKEEREKLG+STGKK+SA+RT
Sbjct: 181 EKLLNNLGGMFSKPWKPKKTKEITGPLITADNSSGRTENNKEEREKLGLSTGKKKSATRT 240

Query: 241 PPSESSGAMQKVEVEKEKQDDALSDLSNILGDLKNMAVDMGSELDRQNKALDHLSDDVDE 300
           PPSESSGAMQKVE EKEKQDDALSDLSNILGDLKNMAVDMGSELD+QNKALDHLSDDVDE
Sbjct: 241 PPSESSGAMQKVEDEKEKQDDALSDLSNILGDLKNMAVDMGSELDKQNKALDHLSDDVDE 300

Query: 301 LNSRVKGANQRARRLVG 304
           LNSRVKGANQRARRL+G
Sbjct: 301 LNSRVKGANQRARRLLG 316

BLAST of Tan0018511 vs. ExPASy TrEMBL
Match: A0A6J1G0W1 (putative SNAP25 homologous protein SNAP30 OS=Cucurbita moschata OX=3662 GN=LOC111449670 PE=4 SV=1)

HSP 1 Score: 509.6 bits (1311), Expect = 8.9e-141
Identity = 278/317 (87.70%), Postives = 284/317 (89.59%), Query Frame = 0

Query: 1   MFSFMKSPAKVSKPNSEDPGLSAGSGTNPFDSDTGLETNQTPNPARRTSSEPALVIPKSN 60
           MFSFMKSP KVSK NS D GL AGSGTNPFDSDT  ETNQT NPARRTSSEPALVIP S 
Sbjct: 1   MFSFMKSPVKVSKQNSVDTGL-AGSGTNPFDSDTEPETNQTLNPARRTSSEPALVIPNSI 60

Query: 61  SPNPF--DDDDDGFVGRRGT------------ATSKPSKDRYKNDFRDSGGLENQSVQEL 120
           S NPF  DDDDDGFVGRRGT            ATS  SK+ YKNDFRDSGG ENQSVQEL
Sbjct: 61  SANPFDDDDDDDGFVGRRGTATSSASKNGYKNATSSASKNGYKNDFRDSGGFENQSVQEL 120

Query: 121 ENYAVYKAEETTKSVNNCLKIAEDIRGDATRTLDMLHQQGEQIERTHRMAADMDKDLSKG 180
           ENYAVYKAEETT SVNNCLKIAEDIR DATRTLDMLHQQGEQIERTHRMAADMDKDLSKG
Sbjct: 121 ENYAVYKAEETTNSVNNCLKIAEDIRSDATRTLDMLHQQGEQIERTHRMAADMDKDLSKG 180

Query: 181 EKLLNNLGGMFSKPWKPKKTREITGPLITADNSSGRADNNKEEREKLGVSTGKKQSASRT 240
           EKLLNNLGGMFSKPWKPKKT+EITGPLITADNSSGR +NNKEEREKLG+STGKKQSA+RT
Sbjct: 181 EKLLNNLGGMFSKPWKPKKTKEITGPLITADNSSGRTENNKEEREKLGLSTGKKQSATRT 240

Query: 241 PPSESSGAMQKVEVEKEKQDDALSDLSNILGDLKNMAVDMGSELDRQNKALDHLSDDVDE 300
           PPSESSGAMQKVE EKEKQDDALSDLSNILGDLKNMAVDMGSELDRQNKALDHLSDDVDE
Sbjct: 241 PPSESSGAMQKVEDEKEKQDDALSDLSNILGDLKNMAVDMGSELDRQNKALDHLSDDVDE 300

Query: 301 LNSRVKGANQRARRLVG 304
           LNSRVKGANQRARRL+G
Sbjct: 301 LNSRVKGANQRARRLLG 316

BLAST of Tan0018511 vs. ExPASy TrEMBL
Match: A0A6J1HWW0 (putative SNAP25 homologous protein SNAP30 OS=Cucurbita maxima OX=3661 GN=LOC111467582 PE=4 SV=1)

HSP 1 Score: 506.5 bits (1303), Expect = 7.6e-140
Identity = 276/317 (87.07%), Postives = 285/317 (89.91%), Query Frame = 0

Query: 1   MFSFMKSPAKVSKPNSEDPGLSAGSGTNPFDSDTGLETNQTPNPARRTSSEPALVIPKSN 60
           MFSFMKSPAKVSK NS DPGL AGSGTNPFDSDT  ETNQ  NPARRTSSEPALVI  S 
Sbjct: 1   MFSFMKSPAKVSKQNSVDPGL-AGSGTNPFDSDTEPETNQPLNPARRTSSEPALVILNSI 60

Query: 61  SPNPF--DDDDDGFVGRRGTATSKPSK------------DRYKNDFRDSGGLENQSVQEL 120
           SPNPF  DDDDDGFVGRRGTATS  SK            + YKNDFRDSGG E+QSVQEL
Sbjct: 61  SPNPFDDDDDDDGFVGRRGTATSSASKNGYKNATSSASINGYKNDFRDSGGFESQSVQEL 120

Query: 121 ENYAVYKAEETTKSVNNCLKIAEDIRGDATRTLDMLHQQGEQIERTHRMAADMDKDLSKG 180
           ENYAVYKAEETT SVNNCLKIAEDIR DATRTLDMLHQQGEQIERTHRMAADMDKDLSKG
Sbjct: 121 ENYAVYKAEETTNSVNNCLKIAEDIRSDATRTLDMLHQQGEQIERTHRMAADMDKDLSKG 180

Query: 181 EKLLNNLGGMFSKPWKPKKTREITGPLITADNSSGRADNNKEEREKLGVSTGKKQSASRT 240
           EKLLNNLGGMFSKPWKPKKT+EITGPLITADNSSGR +NNKEEREKLG+STGKK+SA+RT
Sbjct: 181 EKLLNNLGGMFSKPWKPKKTKEITGPLITADNSSGRTENNKEEREKLGLSTGKKKSATRT 240

Query: 241 PPSESSGAMQKVEVEKEKQDDALSDLSNILGDLKNMAVDMGSELDRQNKALDHLSDDVDE 300
           PPSESSGAMQKVE EKEKQDDALSDLSNILGDLKNMAVDMGSELD+QNKALDHLSDDVDE
Sbjct: 241 PPSESSGAMQKVEDEKEKQDDALSDLSNILGDLKNMAVDMGSELDKQNKALDHLSDDVDE 300

Query: 301 LNSRVKGANQRARRLVG 304
           LNSRVKGANQRARRL+G
Sbjct: 301 LNSRVKGANQRARRLLG 316

BLAST of Tan0018511 vs. ExPASy TrEMBL
Match: A0A0A0KQT5 (t-SNARE coiled-coil homology domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G606590 PE=4 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 8.4e-139
Identity = 265/304 (87.17%), Postives = 283/304 (93.09%), Query Frame = 0

Query: 1   MFSFMKSPAKVSKPNSEDPGLSAGSGTNPFDSDTGLETNQTPNPARRTSSEPALVIPKSN 60
           MFSFMKSPAKV+K NS DP LS GSGTNPFDSDTG +  QT N ARRTSSEP L +PK+ 
Sbjct: 1   MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLPMPKA- 60

Query: 61  SPNPFDDDDD-GFVGRRGTATSKPSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTK 120
             NPFDDDDD GFVGR+GTATS  SKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTK
Sbjct: 61  --NPFDDDDDTGFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTK 120

Query: 121 SVNNCLKIAEDIRGDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180
           SVNNCLKIAEDIR DAT+TLDMLH+QGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK
Sbjct: 121 SVNNCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180

Query: 181 PWKPKKTREITGPLITADNSSGRADNNKEEREKLGVSTGKKQSASRTPPSESSGAMQKVE 240
           PWKPKKT+EITGPLITAD+SSG+ +NNKE+REKLG+STGKKQSA++TPPSE SGA+QKVE
Sbjct: 181 PWKPKKTKEITGPLITADHSSGKTENNKEQREKLGLSTGKKQSATKTPPSEPSGAIQKVE 240

Query: 241 VEKEKQDDALSDLSNILGDLKNMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR 300
           VEKEKQDDALSDLSNILGDLK+MAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR
Sbjct: 241 VEKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR 300

Query: 301 RLVG 304
            L+G
Sbjct: 301 HLIG 301

BLAST of Tan0018511 vs. ExPASy TrEMBL
Match: A0A1S3BF13 (putative SNAP25 homologous protein SNAP30 OS=Cucumis melo OX=3656 GN=LOC103489173 PE=4 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 5.4e-138
Identity = 264/303 (87.13%), Postives = 281/303 (92.74%), Query Frame = 0

Query: 1   MFSFMKSPAKVSKPNSEDPGLSAGSGTNPFDSDTGLETNQTPNPARRTSSEPALVIPKSN 60
           MFSFMKSPAKV+K NS DP LS GSGTNPFDSDTG +  QT N ARRTSSEP L IP + 
Sbjct: 1   MFSFMKSPAKVTKQNSVDPELSTGSGTNPFDSDTGPDAKQTLNAARRTSSEPVLPIPNA- 60

Query: 61  SPNPFDDDDDGFVGRRGTATSKPSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKS 120
             NPF DDDDGFVGR+GTATS  SKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKS
Sbjct: 61  --NPF-DDDDGFVGRKGTATSSGSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKS 120

Query: 121 VNNCLKIAEDIRGDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP 180
           VNNCLKIAEDIR DAT+TLDMLH+QGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP
Sbjct: 121 VNNCLKIAEDIREDATKTLDMLHKQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP 180

Query: 181 WKPKKTREITGPLITADNSSGRADNNKEEREKLGVSTGKKQSASRTPPSESSGAMQKVEV 240
           WKPKKT+EITGPLITAD+SSG+ +NNK++REKLG+STGKKQSA++TPPSE SGAMQKVEV
Sbjct: 181 WKPKKTKEITGPLITADHSSGKTENNKKQREKLGLSTGKKQSATKTPPSEPSGAMQKVEV 240

Query: 241 EKEKQDDALSDLSNILGDLKNMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARR 300
           EKEKQDDALSDLSNILGDLK+MAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR 
Sbjct: 241 EKEKQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARH 299

Query: 301 LVG 304
           L+G
Sbjct: 301 LIG 299

BLAST of Tan0018511 vs. ExPASy TrEMBL
Match: A0A6J1J9F6 (putative SNAP25 homologous protein SNAP30 OS=Cucurbita maxima OX=3661 GN=LOC111482502 PE=4 SV=1)

HSP 1 Score: 497.3 bits (1279), Expect = 4.6e-137
Identity = 265/304 (87.17%), Postives = 280/304 (92.11%), Query Frame = 0

Query: 1   MFSFMKSPAKVSKPNSEDPGLSAGSGTNPFDSDTGLETNQTPNPARRTSSEPALVIPKSN 60
           MFSFMKSPAKV+K NS D  LS GSGTNPFDSD+G E  QT N ARRTSSEP LVIP S 
Sbjct: 1   MFSFMKSPAKVTKQNSVDE-LSGGSGTNPFDSDSGPEAKQTLNAARRTSSEPVLVIPNSI 60

Query: 61  SPNPF-DDDDDGFVGRRGTATSKPSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTK 120
           S N F DDDDDGFVG+RGTATS  SKDRYKNDFRDSGGLENQSVQELENYA+YKAEETTK
Sbjct: 61  SANAFDDDDDDGFVGKRGTATSAASKDRYKNDFRDSGGLENQSVQELENYALYKAEETTK 120

Query: 121 SVNNCLKIAEDIRGDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180
           SVNNCLKIAEDIR DAT+TLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK
Sbjct: 121 SVNNCLKIAEDIREDATKTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSK 180

Query: 181 PWKPKKTREITGPLITADNSSGRADNNKEEREKLGVSTGKKQSASRTPPSESSGAMQKVE 240
           PWKPKKT+EITGPLITAD+SSGR +NNKE+REKLGVSTGKKQSA++TPPSE +GA+QKVE
Sbjct: 181 PWKPKKTKEITGPLITADHSSGRTENNKEQREKLGVSTGKKQSATKTPPSEPTGAIQKVE 240

Query: 241 VEKEKQDDALSDLSNILGDLKNMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR 300
            +KE QDDALSDLSNILGDLK+MAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR
Sbjct: 241 AQKEMQDDALSDLSNILGDLKSMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRAR 300

Query: 301 RLVG 304
            LVG
Sbjct: 301 HLVG 303

BLAST of Tan0018511 vs. TAIR 10
Match: AT1G13890.1 (soluble N-ethylmaleimide-sensitive factor adaptor protein 30 )

HSP 1 Score: 322.8 bits (826), Expect = 3.0e-88
Identity = 182/302 (60.26%), Postives = 212/302 (70.20%), Query Frame = 0

Query: 1   MFSFMKSPAKVSKPNSEDPGLSAGSGTNPFDSDTGLETNQTPNPARRTSSEPALVIPKSN 60
           MF F KSP                 G N   +++      T    RRTSSEP L+ P   
Sbjct: 1   MFGFFKSP-----------------GNNKLPNESSNNKGGTITAGRRTSSEPILITP--- 60

Query: 61  SPNPFDDDDDGFVGRRGTATSKPSKDRYKNDFRDSGGLENQSVQELENYAVYKAEETTKS 120
               FDDD                 D+YKN F DSGGL++Q+ +ELE YAVYKAEETTK 
Sbjct: 61  ---DFDDD-----------------DKYKNGFNDSGGLQSQTTEELEKYAVYKAEETTKG 120

Query: 121 VNNCLKIAEDIRGDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMFSKP 180
           VNNCLKIAEDIR D  RTL+MLHQQGEQI RTH MA DMDKDLS+GEKLLNNLGGMFSKP
Sbjct: 121 VNNCLKIAEDIRSDGARTLEMLHQQGEQINRTHEMAVDMDKDLSRGEKLLNNLGGMFSKP 180

Query: 181 WKPKKTREITGPLITADNSSGRADNNKEEREKLGVSTGKKQSASRTPPSESSGAMQKVEV 240
           WKPKKT+ ITGP+IT D  S +++N+KEEREKLG+   K +S+S+    + + A+QKVE 
Sbjct: 181 WKPKKTKNITGPMITPDKPSKKSENHKEEREKLGLG-AKGRSSSQPALDQPTNALQKVEQ 240

Query: 241 EKEKQDDALSDLSNILGDLKNMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQRARR 300
           EK KQDD LSDLS+ILGDLK+MAVDMGSE+D+QNKALDHL DDVDELNSRV+GANQRAR 
Sbjct: 241 EKAKQDDGLSDLSDILGDLKSMAVDMGSEIDKQNKALDHLGDDVDELNSRVQGANQRARH 261

Query: 301 LV 303
           L+
Sbjct: 301 LL 261

BLAST of Tan0018511 vs. TAIR 10
Match: AT5G61210.1 (soluble N-ethylmaleimide-sensitive factor adaptor protein 33 )

HSP 1 Score: 322.0 bits (824), Expect = 5.1e-88
Identity = 186/309 (60.19%), Postives = 228/309 (73.79%), Query Frame = 0

Query: 1   MFSFMKSPAKVSKPNSEDPGLSAGSGTNPFDSDTGLETNQTPNPARRTSSEPALVIPKSN 60
           MF   KSPA + K NS D      S  NPFDSD   +   T NP++RT+SEP+L    ++
Sbjct: 1   MFGLRKSPANLPKHNSVD---LKSSKPNPFDSDDESDNKHTLNPSKRTTSEPSL----AD 60

Query: 61  SPNPFDDDDDGFVGRRGTATSKP-----SKDRYKNDFRDSGGLENQSVQELENYAVYKAE 120
             NPF  +    V +  +++SK      SK +YKN+FRDSGG+ENQSVQELE YAVYKAE
Sbjct: 61  MTNPFGGER---VQKGDSSSSKQSLFSNSKYQYKNNFRDSGGIENQSVQELEGYAVYKAE 120

Query: 121 ETTKSVNNCLKIAEDIRGDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGG 180
           ETTKSV  CLK+AEDIR DATRTL MLH QGEQI RTH  A ++D DLS+GEKLL +LGG
Sbjct: 121 ETTKSVQGCLKVAEDIRSDATRTLVMLHDQGEQITRTHHKAVEIDHDLSRGEKLLGSLGG 180

Query: 181 MFSKPWKPKKTREITGPLITADNSSGRADNNKEEREKLGVSTGKK-QSASRTPPSESSGA 240
           MFSK WKPKKTR I GP++T D+S  R  N+ E+REKLG+++  + QS +R P  ES+ A
Sbjct: 181 MFSKTWKPKKTRPINGPVVTRDDSPTRRVNHLEKREKLGLNSAPRGQSRTREPLPESADA 240

Query: 241 MQKVEVEKEKQDDALSDLSNILGDLKNMAVDMGSELDRQNKALDHLSDDVDELNSRVKGA 300
            Q+VE+EK KQDD LSDLS+ILG+LKNMAVDMGSE+++QNK LDHL DDVDELN RV+ +
Sbjct: 241 YQRVEMEKAKQDDGLSDLSDILGELKNMAVDMGSEIEKQNKGLDHLHDDVDELNFRVQQS 299

Query: 301 NQRARRLVG 304
           NQR RRL+G
Sbjct: 301 NQRGRRLLG 299

BLAST of Tan0018511 vs. TAIR 10
Match: AT5G07880.1 (synaptosomal-associated protein SNAP25-like 29 )

HSP 1 Score: 231.9 bits (590), Expect = 6.9e-61
Identity = 131/245 (53.47%), Postives = 173/245 (70.61%), Query Frame = 0

Query: 58  KSNSPNPFDDDDDGFVGRRGTATSKPSKDRYKNDFRDSGGLENQSVQELENYAVYKAEET 117
           KS S NPFDDDDD           K  + R+ +  + SGG ENQ+VQELE+YAVY +EET
Sbjct: 19  KSFSLNPFDDDDD----------DKEVEKRFTSSLKPSGGKENQTVQELESYAVYNSEET 78

Query: 118 TKSVNNCLKIAEDIRGDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKLLNNLGGMF 177
           TK+V  CLK+AE+IR DA++TL ML++QG+QI RTH+   D+D  LS+GEK+L  LGG+F
Sbjct: 79  TKTVQGCLKVAEEIRCDASKTLVMLNEQGDQITRTHQKTVDLDHHLSRGEKILGRLGGVF 138

Query: 178 SKPWKPKKTREITGPLITADNSSGRADNNKEEREKLGVSTGKKQSASRTPPSESSGAMQK 237
           S+ WKPKK+R ITGP+IT  +S  R   + + REKLG++   K  +   P  E+  A QK
Sbjct: 139 SRTWKPKKSRSITGPVITKGDSPKRKVIDLKTREKLGLNPSLKPKSKTLP--EAVDAYQK 198

Query: 238 VEVEKEKQDDALSDLSNILGDLKNMAVDMGSELDRQNKALDHLSDDVDELNSRVKGANQR 297
            ++   KQD+AL+DLS +LG+LKNMAVDMG+ ++RQ   LDHL D+ DELN RVK +NQR
Sbjct: 199 TQI--AKQDEALTDLSALLGELKNMAVDMGTAIERQTNELDHLQDNADELNYRVKQSNQR 249

Query: 298 ARRLV 303
           AR L+
Sbjct: 259 ARYLL 249

BLAST of Tan0018511 vs. TAIR 10
Match: AT3G09740.1 (syntaxin of plants 71 )

HSP 1 Score: 49.3 bits (116), Expect = 6.4e-06
Identity = 62/211 (29.38%), Postives = 84/211 (39.81%), Query Frame = 0

Query: 110 AVYKAEETTKSVNNCLKIAEDIRGDATRTLDMLHQQGEQIERTHRMAADMDKDLSKGEKL 169
           A+ KAE  TK  N    +A  +  +  RT   L    E++ +  R+A    K L+  E  
Sbjct: 50  ALEKAELVTKEKNRAAAVA--MNAEIRRTKARL---SEEVPKLQRLAVKRVKGLTTEELA 109

Query: 170 LNN-----------------LGGMFS-KPWKPKKTREITGPLITADNSSGRADNNKEERE 229
             N                  GG  S   W P  T   + P I  D S GR D++  +  
Sbjct: 110 ARNDLVLALPARIEAIPDGTAGGPKSTSAWTPSST--TSRPDIKFD-SDGRFDDDYFQES 169

Query: 230 KLGVSTGKKQSASRTPPSESSGAMQKVEVEKEKQDDALSDLSNILGDLKNMAVDMGSELD 289
                            +ESS   Q+ E+ K KQ+  L  +S  L  LKNMA DM  ELD
Sbjct: 170 -----------------NESSQFRQEYEMRKIKQEQGLDMISEGLDALKNMASDMNEELD 229

Query: 290 RQNKALDHLSDDVDELNSRVKGANQRARRLV 303
           RQ   +D +   VD   S +K  N R +  V
Sbjct: 230 RQVPLMDEIDTKVDRATSDLKNTNVRLKDTV 235

BLAST of Tan0018511 vs. TAIR 10
Match: AT3G45280.1 (syntaxin of plants 72 )

HSP 1 Score: 46.2 bits (108), Expect = 5.4e-05
Identity = 27/71 (38.03%), Postives = 41/71 (57.75%), Query Frame = 0

Query: 230 ESSGAMQKVEVEKEKQDDALSDLSNILGDLKNMAVDMGSELDRQNKALDHLSDDVDELNS 289
           ESS   Q+ E+ ++KQD+ L  +S  L  LKN+A DM  ELD+Q   ++ +   VD   S
Sbjct: 164 ESSQFRQEYEMRRKKQDEGLDIISEGLDALKNLARDMNEELDKQVPLMEEMETKVDGATS 223

Query: 290 RVKGANQRARR 301
            +K  N R ++
Sbjct: 224 DLKNTNVRLKK 234

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LMG84.2e-8760.26Putative SNAP25 homologous protein SNAP30 OS=Arabidopsis thaliana OX=3702 GN=SNA... [more]
Q9S7P97.2e-8760.19SNAP25 homologous protein SNAP33 OS=Arabidopsis thaliana OX=3702 GN=SNAP33 PE=1 ... [more]
Q9SD969.8e-6053.47SNAP25 homologous protein SNAP29 OS=Arabidopsis thaliana OX=3702 GN=SNAP29 PE=2 ... [more]
P369773.9e-1629.05Synaptosomal-associated protein 25-A OS=Carassius auratus OX=7957 GN=snap25a PE=... [more]
Q17QQ33.3e-1528.37Synaptosomal-associated protein 25 OS=Bos taurus OX=9913 GN=SNAP25 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_038891847.14.0e-14388.78putative SNAP25 homologous protein SNAP30 [Benincasa hispida] >XP_038891848.1 pu... [more]
XP_022945438.11.8e-14087.70putative SNAP25 homologous protein SNAP30 [Cucurbita moschata] >XP_022945439.1 p... [more]
XP_023542548.12.4e-14087.42putative SNAP25 homologous protein SNAP30 [Cucurbita pepo subsp. pepo][more]
KAG6574050.11.2e-13986.83SNAP25-likeous protein SNAP33, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022968314.11.6e-13987.07putative SNAP25 homologous protein SNAP30 [Cucurbita maxima] >XP_022968315.1 put... [more]
Match NameE-valueIdentityDescription
A0A6J1G0W18.9e-14187.70putative SNAP25 homologous protein SNAP30 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1HWW07.6e-14087.07putative SNAP25 homologous protein SNAP30 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A0A0KQT58.4e-13987.17t-SNARE coiled-coil homology domain-containing protein OS=Cucumis sativus OX=365... [more]
A0A1S3BF135.4e-13887.13putative SNAP25 homologous protein SNAP30 OS=Cucumis melo OX=3656 GN=LOC10348917... [more]
A0A6J1J9F64.6e-13787.17putative SNAP25 homologous protein SNAP30 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT1G13890.13.0e-8860.26soluble N-ethylmaleimide-sensitive factor adaptor protein 30 [more]
AT5G61210.15.1e-8860.19soluble N-ethylmaleimide-sensitive factor adaptor protein 33 [more]
AT5G07880.16.9e-6153.47synaptosomal-associated protein SNAP25-like 29 [more]
AT3G09740.16.4e-0629.38syntaxin of plants 71 [more]
AT3G45280.15.4e-0538.03syntaxin of plants 72 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 270..297
NoneNo IPR availableGENE3D1.20.5.110coord: 97..175
e-value: 1.5E-21
score: 78.1
NoneNo IPR availableGENE3D1.20.5.110coord: 236..303
e-value: 9.0E-24
score: 85.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 63..94
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 202..216
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..99
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 217..233
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 181..247
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 18..57
NoneNo IPR availablePANTHERPTHR19305:SF25SNAP25 HOMOLOGOUS PROTEIN SNAP30-RELATEDcoord: 1..302
NoneNo IPR availablePANTHERPTHR19305SYNAPTOSOMAL ASSOCIATED PROTEINcoord: 1..302
NoneNo IPR availableCDDcd15841SNARE_Qccoord: 242..300
e-value: 6.07842E-18
score: 74.132
NoneNo IPR availableSUPERFAMILY58038SNARE fusion complexcoord: 234..301
NoneNo IPR availableSUPERFAMILY58038SNARE fusion complexcoord: 101..172
IPR000727Target SNARE coiled-coil homology domainSMARTSM00397tSNARE_6coord: 106..173
e-value: 0.074
score: 22.2
coord: 234..301
e-value: 1.9E-11
score: 54.0
IPR000727Target SNARE coiled-coil homology domainPROSITEPS50192T_SNAREcoord: 239..301
score: 17.511843
IPR044766NPSN/SNAP25-like, N-terminal SNARE domainCDDcd15861SNARE_SNAP25N_23N_29N_SEC9Ncoord: 108..172
e-value: 2.17005E-21
score: 83.7767

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0018511.1Tan0018511.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0061025 membrane fusion
biological_process GO:0015031 protein transport
cellular_component GO:0005886 plasma membrane
cellular_component GO:0031201 SNARE complex
molecular_function GO:0005484 SNAP receptor activity