Tan0015432 (gene) Snake gourd v1

BLAST of Tan0015432 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 4.4e-148
Identity = 266/517 (51.45%), Postives = 363/517 (70.21%), Query Frame = 0

Query: 27  ADIHQDFLHCLSEHYSSDSYSISKVIYTPINSSYSSVLDFSIRNLRFSTADTPKPLVIIT 86
           A+  ++FL C S+ Y   + + +K++YT  +  Y S+L+ +I+NLRF++  TPKPLVIIT
Sbjct: 28  ANPQENFLKCFSQ-YIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPLVIIT 87

Query: 87  PLYVSHIQAAIICSKNHGLQIRTRSGGHDFEGLSYVSHLPFIIVDLINLKSIQIDVNKST 146
           PL VSHIQ  I+CSK  GLQIRTRSGGHD EG+SY+S +PF+IVDL N+ S++IDV+  T
Sbjct: 88  PLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQT 147

Query: 147 AWVQSGATLGELYYTIAEKSRTLAFPAGSCLTIGIGGHFSGGGRGLMTRKYGLAADNVID 206
           AWV++GATLGE+YY I E +  L+FPAG C T+G GGHFSGGG G + R YGLAADN+ID
Sbjct: 148 AWVEAGATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAADNIID 207

Query: 207 ATLVDANGKIHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICSTNRNLE- 266
           A LV+ +GK+ DR+SMGEDLFWAIRGGGG +FGI+ AWK++LV VP+  TI S  +N+E 
Sbjct: 208 AHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEI 267

Query: 267 EDAIMLIHRWQYVANKLDENLFLGIRLSGGNISTQQGITNPT--ATFVSFFLGKVDELVA 326
            + + L+++WQ +A   ++ L L       NI+  QG    T  + F S F G VD LV 
Sbjct: 268 HELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVD 327

Query: 327 IIKTTFPELGLKKKDCVETSWIESTLIAANGI----QTVESLEPLLNRTPSAEGSTKVKS 386
           ++  +FPELG+KK DC + SWI+ T+I  +G+     T    E LL+R+   + +  +K 
Sbjct: 328 LMNKSFPELGIKKTDCKQLSWID-TIIFYSGVVNYNTTYFKKEILLDRSGGRKAAFSIKL 387

Query: 387 DFIKEPIPKAAIEGLWQRLKAQDVKAS-YQFIPNGGIMSQISESETPFPYRAGNLYGIVY 446
           D++K+PIP+ A+  + ++L  +DV    + F P GGIM +ISES  PFP+RAG +Y I Y
Sbjct: 388 DYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMYEIWY 447

Query: 447 FARWKEQSENAKKRHFNWIREVYDYMAPFVSKSPRAAYVNSRDLDIGTNDKYGKTSYKRA 506
            A W++Q +N K  H NWIR VY++  P+VS++PR AY+N RDLD+G  +     +Y +A
Sbjct: 448 IASWEKQEDNEK--HINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQA 507

Query: 507 SVWGLKYFGNNFNRLVYVKTKVDPYDFFRHEQSIPTL 536
            +WG KYFG NFNRLV VKTKVDP +FFR+EQSIP L
Sbjct: 508 RIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of Tan0015432 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 9.9e-148
Identity = 265/517 (51.26%), Postives = 362/517 (70.02%), Query Frame = 0

Query: 27  ADIHQDFLHCLSEHYSSDSYSISKVIYTPINSSYSSVLDFSIRNLRFSTADTPKPLVIIT 86
           A+  ++FL CLS+ Y   + + +K++YT  +  Y S+L+ +++NLRF++  TPKPLVI T
Sbjct: 28  ANPQENFLKCLSQ-YIPTNVTNAKLVYTQHDQFYMSILNSTVQNLRFTSDTTPKPLVITT 87

Query: 87  PLYVSHIQAAIICSKNHGLQIRTRSGGHDFEGLSYVSHLPFIIVDLINLKSIQIDVNKST 146
           PL VSHIQ  I+CSK  GLQIRTRSGGHD EG+SY+S +PF+IVDL N+ S++IDV+  T
Sbjct: 88  PLNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQT 147

Query: 147 AWVQSGATLGELYYTIAEKSRTLAFPAGSCLTIGIGGHFSGGGRGLMTRKYGLAADNVID 206
           AWV+SGATLGE+YY I E +  L+FPAG C T+G GGHFSGGG G + R YGLAADN+ID
Sbjct: 148 AWVESGATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNYGLAADNIID 207

Query: 207 ATLVDANGKIHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICSTNRNLE- 266
           A LV+ +GK+ DR+SMGEDLFWAIRGGGG +FGI+ AWK++LV VP+  TI S  +N+E 
Sbjct: 208 AHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEI 267

Query: 267 EDAIMLIHRWQYVANKLDENLFLGIRLSGGNISTQQGITNPT--ATFVSFFLGKVDELVA 326
            + + L+++WQ +A   ++ L L       NI+  QG    T  + F S F G VD LV 
Sbjct: 268 HELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVD 327

Query: 327 IIKTTFPELGLKKKDCVETSWIESTLIAANGIQTVESL----EPLLNRTPSAEGSTKVKS 386
           ++  +FPELG+KK DC + SWI+ T+I  +G+    +     E LL+R+   + +  +K 
Sbjct: 328 LMNKSFPELGIKKTDCKQLSWID-TIIFYSGVVNYNTTNFKKEILLDRSGGRKAAFSIKL 387

Query: 387 DFIKEPIPKAAIEGLWQRLKAQDVKAS-YQFIPNGGIMSQISESETPFPYRAGNLYGIVY 446
           D++K+PIP+ A+  + ++L  +DV    + F P GGIM +ISES  PFP+RAG  Y I Y
Sbjct: 388 DYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGITYEIWY 447

Query: 447 FARWKEQSENAKKRHFNWIREVYDYMAPFVSKSPRAAYVNSRDLDIGTNDKYGKTSYKRA 506
            A W++Q +N K  H NWIR VY++  P+VS++PR AY+N RDLD+G  +     +Y +A
Sbjct: 448 IASWEKQEDNEK--HINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQA 507

Query: 507 SVWGLKYFGNNFNRLVYVKTKVDPYDFFRHEQSIPTL 536
            +WG KYFG NFNRLV VKTKVDP +FFR+EQSIP L
Sbjct: 508 RIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of Tan0015432 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 2.9e-147
Identity = 263/516 (50.97%), Postives = 359/516 (69.57%), Query Frame = 0

Query: 27  ADIHQDFLHCLSEHYSSDSYSISKVIYTPINSSYSSVLDFSIRNLRFSTADTPKPLVIIT 86
           A+  ++FL C S+H   ++ +  K++YT  +  Y S+L+ +I+NLRF +  TPKPLVI+T
Sbjct: 28  ANPRENFLKCFSKHI-PNNVANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVT 87

Query: 87  PLYVSHIQAAIICSKNHGLQIRTRSGGHDFEGLSYVSHLPFIIVDLINLKSIQIDVNKST 146
           P   SHIQA I+CSK  GLQIRTRSGGHD EG+SY+S +PF++VDL N+ SI+IDV+  T
Sbjct: 88  PSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQT 147

Query: 147 AWVQSGATLGELYYTIAEKSRTLAFPAGSCLTIGIGGHFSGGGRGLMTRKYGLAADNVID 206
           AWV++GATLGE+YY I EK+  L+FP G C T+G+GGHFSGGG G + R YGLAADN+ID
Sbjct: 148 AWVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIID 207

Query: 207 ATLVDANGKIHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICSTNRNLE- 266
           A LV+ +GK+ DR+SMGEDLFWAIRGGGG +FGI+ AWK+KLV VP+  TI S  +N+E 
Sbjct: 208 AHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEI 267

Query: 267 EDAIMLIHRWQYVANKLDENLFLGIRLSGGNISTQQGITNPT--ATFVSFFLGKVDELVA 326
              + L ++WQ +A K D++L L       NI+   G    T    F S F G VD LV 
Sbjct: 268 HGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFSSIFHGGVDSLVD 327

Query: 327 IIKTTFPELGLKKKDCVETSWIESTLIAANGIQTVES---LEPLLNRTPSAEGSTKVKSD 386
           ++  +FPELG+KK DC E SWI++T+  +  +    +    E LL+R+   + +  +K D
Sbjct: 328 LMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLDRSAGKKTAFSIKLD 387

Query: 387 FIKEPIPKAAIEGLWQRLKAQDVKAS-YQFIPNGGIMSQISESETPFPYRAGNLYGIVYF 446
           ++K+PIP+ A+  + ++L  +DV A  Y   P GGIM +ISES  PFP+RAG +Y + Y 
Sbjct: 388 YVKKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGIMYELWYT 447

Query: 447 ARWKEQSENAKKRHFNWIREVYDYMAPFVSKSPRAAYVNSRDLDIGTNDKYGKTSYKRAS 506
           A W++Q +N K  H NW+R VY++  P+VS++PR AY+N RDLD+G  +     +Y +A 
Sbjct: 448 ASWEKQEDNEK--HINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNHASPNNYTQAR 507

Query: 507 VWGLKYFGNNFNRLVYVKTKVDPYDFFRHEQSIPTL 536
           +WG KYFG NFNRLV VKTKVDP +FFR+EQSIP L
Sbjct: 508 IWGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPL 540

BLAST of Tan0015432 vs. ExPASy Swiss-Prot
Match: A6P6V9 (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 3.8e-147
Identity = 266/518 (51.35%), Postives = 366/518 (70.66%), Query Frame = 0

Query: 27  ADIHQDFLHCLSEHYSSDSYSISKVIYTPINSSYSSVLDFSIRNLRFSTADTPKPLVIIT 86
           A+  ++FL C S++  +++ ++ K++YT  N  Y SVL+ +I NLRF++  TPKPLVI+T
Sbjct: 28  ANPRENFLKCFSQYIPNNATNL-KLVYTQNNPLYMSVLNSTIHNLRFTSDTTPKPLVIVT 87

Query: 87  PLYVSHIQAAIICSKNHGLQIRTRSGGHDFEGLSYVSHLPFIIVDLINLKSIQIDVNKST 146
           P +VSHIQ  I+CSK  GLQIRTRSGGHD EG+SY+S +PF+IVDL N++SI+IDV+  T
Sbjct: 88  PSHVSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYISQVPFVIVDLRNMRSIKIDVHSQT 147

Query: 147 AWVQSGATLGELYYTIAEKSRTLAFPAGSCLTIGIGGHFSGGGRGLMTRKYGLAADNVID 206
           AWV++GATLGE+YY + EK+  L+  AG C T+  GGHF GGG G + R YGLAADN+ID
Sbjct: 148 AWVEAGATLGEVYYWVNEKNENLSLAAGYCPTVCAGGHFGGGGYGPLMRNYGLAADNIID 207

Query: 207 ATLVDANGKIHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICSTNRNLE- 266
           A LV+ +GK+ DR+SMGEDLFWA+RGGG  SFGI+VAWK++LV VP + T+ S  + +E 
Sbjct: 208 AHLVNVHGKVLDRKSMGEDLFWALRGGGAESFGIIVAWKIRLVAVPKS-TMFSVKKIMEI 267

Query: 267 EDAIMLIHRWQYVANKLDENLFLGIRLSGGNISTQQGITNPTAT---FVSFFLGKVDELV 326
            + + L+++WQ +A K D++L L       NI+  QG  N TA    F S FLG VD LV
Sbjct: 268 HELVKLVNKWQNIAYKYDKDLLLMTHFITRNITDNQG-KNKTAIHTYFSSVFLGGVDSLV 327

Query: 327 AIIKTTFPELGLKKKDCVETSWIESTLIAANGIQTVE----SLEPLLNRTPSAEGSTKVK 386
            ++  +FPELG+KK DC + SWI+ T+I  +G+   +    + E LL+R+    G+ K+K
Sbjct: 328 DLMNKSFPELGIKKTDCRQLSWID-TIIFYSGVVNYDTDNFNKEILLDRSAGQNGAFKIK 387

Query: 387 SDFIKEPIPKAAIEGLWQRLKAQDVKAS-YQFIPNGGIMSQISESETPFPYRAGNLYGIV 446
            D++K+PIP++    + ++L  +D+ A  Y   P GGIM +ISES  PFP+RAG LY + 
Sbjct: 388 LDYVKKPIPESVFVQILEKLYEEDIGAGMYALYPYGGIMDEISESAIPFPHRAGILYELW 447

Query: 447 YFARWKEQSENAKKRHFNWIREVYDYMAPFVSKSPRAAYVNSRDLDIGTNDKYGKTSYKR 506
           Y   W++Q +N K  H NWIR +Y++M P+VSK+PR AY+N RDLDIG ND     +Y +
Sbjct: 448 YICSWEKQEDNEK--HLNWIRNIYNFMTPYVSKNPRLAYLNYRDLDIGINDPKNPNNYTQ 507

Query: 507 ASVWGLKYFGNNFNRLVYVKTKVDPYDFFRHEQSIPTL 536
           A +WG KYFG NF+RLV VKT VDP +FFR+EQSIP L
Sbjct: 508 ARIWGEKYFGKNFDRLVKVKTLVDPNNFFRNEQSIPPL 539

BLAST of Tan0015432 vs. ExPASy Swiss-Prot
Match: Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 2.1e-145
Identity = 260/516 (50.39%), Postives = 356/516 (68.99%), Query Frame = 0

Query: 27  ADIHQDFLHCLSEHYSSDSYSISKVIYTPINSSYSSVLDFSIRNLRFSTADTPKPLVIIT 86
           A+  ++FL C SE Y  ++ +  K IYT  +  Y SVL+ +I+NLRF++  TPKPLVI+T
Sbjct: 28  ANPQENFLKCFSE-YIPNNPANPKFIYTQHDQLYMSVLNSTIQNLRFTSDTTPKPLVIVT 87

Query: 87  PLYVSHIQAAIICSKNHGLQIRTRSGGHDFEGLSYVSHLPFIIVDLINLKSIQIDVNKST 146
           P  VSHIQA+I+CSK  GLQIRTRSGGHD EGLSY+S +PF IVDL N+ ++++D++  T
Sbjct: 88  PSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFAIVDLRNMHTVKVDIHSQT 147

Query: 147 AWVQSGATLGELYYTIAEKSRTLAFPAGSCLTIGIGGHFSGGGRGLMTRKYGLAADNVID 206
           AWV++GATLGE+YY I E +   +FP G C T+G+GGHFSGGG G + R YGLAADN+ID
Sbjct: 148 AWVEAGATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIID 207

Query: 207 ATLVDANGKIHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICSTNRNLE- 266
           A LV+ +GK+ DR+SMGEDLFWAIRGGGG +FGI+ AWK+KLV VP+  TI S  +N+E 
Sbjct: 208 AHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVVVPSKATIFSVKKNMEI 267

Query: 267 EDAIMLIHRWQYVANKLDENLFLGIRLSGGNISTQQGITNPT--ATFVSFFLGKVDELVA 326
              + L ++WQ +A K D++L L       NI+   G    T    F S FLG VD LV 
Sbjct: 268 HGLVKLFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNKTTVHGYFSSIFLGGVDSLVD 327

Query: 327 IIKTTFPELGLKKKDCVETSWIESTLIAANGIQTVES---LEPLLNRTPSAEGSTKVKSD 386
           ++  +FPELG+KK DC E SWI++T+  +  +    +    E LL+R+   + +  +K D
Sbjct: 328 LMNKSFPELGIKKTDCKELSWIDTTIFYSGVVNYNTANFKKEILLDRSAGKKTAFSIKLD 387

Query: 387 FIKEPIPKAAIEGLWQRLKAQDVKAS-YQFIPNGGIMSQISESETPFPYRAGNLYGIVYF 446
           ++K+ IP+ A+  + ++L  ++V    Y   P GGIM +ISES  PFP+RAG +Y + Y 
Sbjct: 388 YVKKLIPETAMVKILEKLYEEEVGVGMYVLYPYGGIMDEISESAIPFPHRAGIMYELWYT 447

Query: 447 ARWKEQSENAKKRHFNWIREVYDYMAPFVSKSPRAAYVNSRDLDIGTNDKYGKTSYKRAS 506
           A W++Q +N K  H NW+R VY++  P+VS++PR AY+N RDLD+G  +     +Y +A 
Sbjct: 448 ATWEKQEDNEK--HINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNPESPNNYTQAR 507

Query: 507 VWGLKYFGNNFNRLVYVKTKVDPYDFFRHEQSIPTL 536
           +WG KYFG NFNRLV VKTK DP +FFR+EQSIP L
Sbjct: 508 IWGEKYFGKNFNRLVKVKTKADPNNFFRNEQSIPPL 540

BLAST of Tan0015432 vs. NCBI nr
Match: XP_038895543.1 (berberine bridge enzyme-like 18 [Benincasa hispida])

HSP 1 Score: 843.2 bits (2177), Expect = 1.3e-240
Identity = 415/537 (77.28%), Postives = 468/537 (87.15%), Query Frame = 0

Query: 1   MKYSS-LIPLSLVLIILSPSPLWVAASADIHQDFLHCLSEHYSSDSYSISKVIYTPINSS 60
           MK+SS LIPL+LVLI+L   PLW AAS   +Q FL CLSEH SS+SY ISKV+Y+PINSS
Sbjct: 1   MKFSSPLIPLALVLIVLYSCPLWAAASPQKYQHFLQCLSEH-SSNSYPISKVVYSPINSS 60

Query: 61  YSSVLDFSIRNLRFSTADTPKPLVIITPLYVSHIQAAIICSKNHGLQIRTRSGGHDFEGL 120
           YSSVLDFSI+NLRFS   TPKPL+IITP +VSHIQAA+ICSK+HGLQIRTRSGGHDFEGL
Sbjct: 61  YSSVLDFSIKNLRFSKPQTPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120

Query: 121 SYVSHLPFIIVDLINLKSIQIDVNKSTAWVQSGATLGELYYTIAEKSRTLAFPAGSCLTI 180
           SYV++ PFI+VDLINL+SI +D   +TAWV+SGATLGELYY I EKSRTLAFPAG C T+
Sbjct: 121 SYVAYRPFIVVDLINLRSISVDDENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTV 180

Query: 181 GIGGHFSGGGRGLMTRKYGLAADNVIDATLVDANGKIHDRESMGEDLFWAIRGGGGGSFG 240
           GIGGHFSGGG GLM RK+GLAADNVIDA LVDANGK+HDRESMGEDLFWAIRGGGGGSFG
Sbjct: 181 GIGGHFSGGGYGLMLRKFGLAADNVIDAHLVDANGKVHDRESMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPATVTICSTNRNLEEDAIMLIHRWQYVANKLDENLFLGIRLSGGNIST 300
           IVVAWK+KLVPVPATVTICST RNLEEDAI L+HRWQYVANKLDENL+LGI L+GG  ST
Sbjct: 241 IVVAWKIKLVPVPATVTICSTQRNLEEDAIKLVHRWQYVANKLDENLYLGIILTGGKAST 300

Query: 301 QQGITNPTATFVSFFLGKVDELVAIIKTTFPELGLKKKDCVETSWIESTLIAANGIQTVE 360
           Q GITNPTATF S FLG++DEL+A + TTFPELGL K+DCVE +W+ESTLI A G+Q +E
Sbjct: 301 QGGITNPTATFFSLFLGRIDELLATLSTTFPELGLIKQDCVEATWVESTLIIATGVQPIE 360

Query: 361 SLEPLLNRTPSAEGSTKVKSDFIKEPIPKAAIEGLWQRLKAQDVKASYQ-FIPNGGIMSQ 420
           SLEPLLNRTP    +TK+KSD++KEP+P+AAIEG+ QRLKAQD++ S   F+P GG MSQ
Sbjct: 361 SLEPLLNRTPPTIENTKIKSDYVKEPLPEAAIEGICQRLKAQDIETSQLIFVPYGGKMSQ 420

Query: 421 ISESETPFPYRAGNLYGIVYFARWKEQSENAKKRHFNWIREVYDYMAPFVSKSPRAAYVN 480
           ISESETPF +RAGNLY I Y   WKEQS  AKKRH +WIREVY+YM PFVSKSPRAAY N
Sbjct: 421 ISESETPFSHRAGNLYKIGYVVGWKEQSLKAKKRHIHWIREVYEYMIPFVSKSPRAAYAN 480

Query: 481 SRDLDIGTNDKYGKTSYKRASVWGLKYFGNNFNRLVYVKTKVDPYDFFRHEQSIPTL 536
            RDLDIG N+KYG+TS ++ASVWGLKYFGNNF +LVYVKTKVDPYDFFRHEQSIPTL
Sbjct: 481 YRDLDIGANNKYGQTSVEQASVWGLKYFGNNFKKLVYVKTKVDPYDFFRHEQSIPTL 536

BLAST of Tan0015432 vs. NCBI nr
Match: XP_038895508.1 (berberine bridge enzyme-like 28 [Benincasa hispida])

HSP 1 Score: 842.0 bits (2174), Expect = 2.8e-240
Identity = 423/538 (78.62%), Postives = 466/538 (86.62%), Query Frame = 0

Query: 1   MKYSS-LIPLSLVLIILSPSPLWVAASADIHQDFLHCLSEHYSSDSYSISKVIYTPINSS 60
           MKY+S LI LSLVLI++S SPLW +AS   +Q FL CL EH S D+Y ISKV+Y+PINSS
Sbjct: 1   MKYTSPLILLSLVLIVVSSSPLWASASEQKYQHFLQCLLEH-SLDNYPISKVVYSPINSS 60

Query: 61  YSSVLDFSIRNLRFSTADTPKPLVIITPLYVSHIQAAIICSKNHGLQIRTRSGGHDFEGL 120
           YSSVLDFSIRNLRFS   TPKPL+IITP +VSHIQAA+ICSK+HGLQIRTRSGGHDFEGL
Sbjct: 61  YSSVLDFSIRNLRFSKPQTPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120

Query: 121 SYVSHLPFIIVDLINLKSIQIDVNKSTAWVQSGATLGELYYTIAEKSRTLAFPAGSCLTI 180
           SYV++LPFIIVDLINL+S+ IDV  STAWVQSGATLGELYY I EKSRTLAFPAG C T+
Sbjct: 121 SYVAYLPFIIVDLINLRSVTIDVENSTAWVQSGATLGELYYRIGEKSRTLAFPAGVCPTV 180

Query: 181 GIGGHFSGGGRGLMTRKYGLAADNVIDATLVDANGKIHDRESMGEDLFWAIRGGGGGSFG 240
           G+GGHFSGGG G M RK+GLAADNVIDA LVDANGK+HDRESMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGYGFMLRKFGLAADNVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPATVTICSTNRNLEEDAIMLIHRWQYVANKLDENLFLGIRLSGGNI-S 300
           IVVAWKVKLVPVPATVT CST+RNLE DAI L+H+WQYVANKLDENLFLGI L+GGNI +
Sbjct: 241 IVVAWKVKLVPVPATVTSCSTHRNLEGDAIKLVHQWQYVANKLDENLFLGILLTGGNITT 300

Query: 301 TQQGITNPTATFVSFFLGKVDELVAIIKTTFPELGLKKKDCVETSWIESTLIAANGIQTV 360
           TQ+GI +PTATF   FLGKVDELVA + TTFPEL L K+DCVETSWIESTLIAA G+Q V
Sbjct: 301 TQEGIADPTATFFILFLGKVDELVATLSTTFPELSLMKQDCVETSWIESTLIAATGVQIV 360

Query: 361 ESLEPLLNRTPSAEGSTKVKSDFIKEPIPKAAIEGLWQRLKAQDVKASYQ-FIPNGGIMS 420
           ESLEPLLNRTP    STK+KSD+IKEPIP+A IEG++QRLKAQD+  S   F+P GG MS
Sbjct: 361 ESLEPLLNRTPPTIESTKIKSDYIKEPIPEATIEGIFQRLKAQDIATSQLIFVPYGGRMS 420

Query: 421 QISESETPFPYRAGNLYGIVYFARWKEQSENAKKRHFNWIREVYDYMAPFVSKSPRAAYV 480
           QISESETPF +RA NLY I Y   WKEQS NAKKRH +WIREVY+YM PFVSKSPR+AY 
Sbjct: 421 QISESETPFSHRAANLYKIGYIVGWKEQSLNAKKRHISWIREVYEYMTPFVSKSPRSAYA 480

Query: 481 NSRDLDIGTNDKYGKTSYKRASVWGLKYFGNNFNRLVYVKTKVDPYDFFRHEQSIPTL 536
           N RDLDIG N+KY KTS K+ASVWGLKYFGNNF RLVYVKTKVDPYDFFRHEQSIP L
Sbjct: 481 NYRDLDIGANNKYRKTSVKQASVWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIPAL 537

BLAST of Tan0015432 vs. NCBI nr
Match: XP_004151192.3 (berberine bridge enzyme-like 18 [Cucumis sativus] >KAE8653316.1 hypothetical protein Csa_023212 [Cucumis sativus])

HSP 1 Score: 832.0 bits (2148), Expect = 2.9e-237
Identity = 413/538 (76.77%), Postives = 467/538 (86.80%), Query Frame = 0

Query: 1   MKYSS-LIPLSLVLIILSPSPLWVAASADIHQDFLHCLSEHYSSDSYSISKVIYTPINSS 60
           MKYSS LIP SL+LI++S S LWV+AS   H +FL CLSEH SS SY ISKV++TPINSS
Sbjct: 1   MKYSSPLIPFSLILIVVSSSSLWVSASPHKHHNFLQCLSEH-SSKSYPISKVVHTPINSS 60

Query: 61  YSSVLDFSIRNLRFSTADTPKPLVIITPLYVSHIQAAIICSKNHGLQIRTRSGGHDFEGL 120
           YSSVL+FSIRNLRFS  +TPKPL+IITP +VSHIQAA+ICSK+HGLQIRTRSGGHD+EGL
Sbjct: 61  YSSVLNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEGL 120

Query: 121 SYVSHLPFIIVDLINLKSIQIDVNKSTAWVQSGATLGELYYTIAEKSRTLAFPAGSCLTI 180
           SYV+  PFII+DLINL+S++IDV  +TAWV+SG TLGELYY I EKSRTLAFPAG C T+
Sbjct: 121 SYVASHPFIILDLINLRSMKIDVESNTAWVESGTTLGELYYRIGEKSRTLAFPAGICPTV 180

Query: 181 GIGGHFSGGGRGLMTRKYGLAADNVIDATLVDANGKIHDRESMGEDLFWAIRGGGGGSFG 240
           G+GGHFSGGG GLM RK+GLAADNVIDA LVDANGK+HDRESMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPATVTICSTNRNLEEDAIMLIHRWQYVANKLDENLFLGIRLSGGNI-S 300
           IVVAWK+KLV VPATVT+C+T RNL+ D I L+H+WQYVANKLDENLFLGI L+GGNI +
Sbjct: 241 IVVAWKIKLVSVPATVTMCNTYRNLKGDGIKLVHQWQYVANKLDENLFLGIILTGGNITT 300

Query: 301 TQQGITNPTATFVSFFLGKVDELVAIIKTTFPELGLKKKDCVETSWIESTLIAANGIQTV 360
           TQ+GITNP ATF S FLGKV+ELVA + TTFPELGL K+DC+ETSWIESTLIA+ G+QTV
Sbjct: 301 TQEGITNPIATFFSLFLGKVNELVATLSTTFPELGLIKQDCIETSWIESTLIASTGVQTV 360

Query: 361 ESLEPLLNRTPSAEGSTKVKSDFIKEPIPKAAIEGLWQRLKAQDVKASYQ-FIPNGGIMS 420
           ESLEPLLNRTPS   + K+KSD+IKEPI  A IEG+WQRLKAQD++ S   FIP GG MS
Sbjct: 361 ESLEPLLNRTPSNLENEKIKSDYIKEPISIATIEGIWQRLKAQDIETSQLIFIPYGGRMS 420

Query: 421 QISESETPFPYRAGNLYGIVYFARWKEQSENAKKRHFNWIREVYDYMAPFVSKSPRAAYV 480
           QISESETPF +R GNLY I Y   WKEQS  AKK+H +WIRE+Y+YM PFVSKSPR+AY 
Sbjct: 421 QISESETPFSHRVGNLYKIGYILGWKEQSLKAKKKHISWIREIYEYMTPFVSKSPRSAYA 480

Query: 481 NSRDLDIGTNDKYGKTSYKRASVWGLKYFGNNFNRLVYVKTKVDPYDFFRHEQSIPTL 536
           N RDLDIG N KYGKTS K+AS+WGLKYFGNNF RLVYVKTKVDPYDFFRHEQSIPTL
Sbjct: 481 NYRDLDIGVNKKYGKTSVKQASIWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIPTL 537

BLAST of Tan0015432 vs. NCBI nr
Match: XP_023520318.1 (berberine bridge enzyme-like 18 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 832.0 bits (2148), Expect = 2.9e-237
Identity = 407/529 (76.94%), Postives = 463/529 (87.52%), Query Frame = 0

Query: 7   IPLSLV-LIILSPSPLWVAASADIHQDFLHCLSEHYSSDSYSISKVIYTPINSSYSSVLD 66
           IPL+L+ +I+LS SPLW + S  IHQ FLHCLS H+S  +Y ISK++YTPINSSYSSVL+
Sbjct: 4   IPLTLIFIIVLSSSPLWASPSLHIHQHFLHCLS-HHSPHTYPISKLVYTPINSSYSSVLN 63

Query: 67  FSIRNLRFSTADTPKPLVIITPLYVSHIQAAIICSKNHGLQIRTRSGGHDFEGLSYVSHL 126
           FSIRNL FS   TPKPL+IITP ++SHIQAA+ICSK H LQIRTRSGGHDFEGLSYV+  
Sbjct: 64  FSIRNLIFSNPQTPKPLLIITPSHISHIQAAVICSKAHDLQIRTRSGGHDFEGLSYVADQ 123

Query: 127 PFIIVDLINLKSIQIDVNKSTAWVQSGATLGELYYTIAEKSRTLAFPAGSCLTIGIGGHF 186
           PFIIVDLINL+SI +DV  +TAWVQSGATLGELYY I EKSR+LAFPAG C T+G+GGHF
Sbjct: 124 PFIIVDLINLRSINVDVQTNTAWVQSGATLGELYYRIGEKSRSLAFPAGICPTVGVGGHF 183

Query: 187 SGGGRGLMTRKYGLAADNVIDATLVDANGKIHDRESMGEDLFWAIRGGGGGSFGIVVAWK 246
           SGGG GLM RKYGLAADNV+DA LVDANG++HDRESMGEDLFWAIRGGGGGSFGIVVAWK
Sbjct: 184 SGGGYGLMLRKYGLAADNVVDAYLVDANGRVHDRESMGEDLFWAIRGGGGGSFGIVVAWK 243

Query: 247 VKLVPVPATVTICSTNRNLEEDAIMLIHRWQYVANKLDENLFLGIRLSGGNISTQQGITN 306
           VKLV VPA VT+CSTNR++E+DAI LIHRWQYVAN+LD+NLFLGI L+GGN ST+QGITN
Sbjct: 244 VKLVVVPAVVTMCSTNRDVEDDAIKLIHRWQYVANQLDDNLFLGILLTGGNFSTRQGITN 303

Query: 307 PTATFVSFFLGKVDELVAIIKTTFPELGLKKKDCVETSWIESTLIAANGIQTVESLEPLL 366
           PTATF S +LGK+DEL+AI+KTTFPELGL KKDCVE+SWI+STLIA+ G+QT E LE LL
Sbjct: 304 PTATFFSLYLGKLDELIAILKTTFPELGLTKKDCVESSWIQSTLIASTGVQTFEPLEALL 363

Query: 367 NRTPSAEGSTKVKSDFIKEPIPKAAIEGLWQRLKAQDVKA-SYQFIPNGGIMSQISESET 426
           NRTP    STK KSD++K+PIP+AAIEG+WQRLKAQD++     FIP GG MSQISESET
Sbjct: 364 NRTPPTIESTKFKSDYVKDPIPEAAIEGIWQRLKAQDIEVPQILFIPYGGRMSQISESET 423

Query: 427 PFPYRAGNLYGIVYFARWKEQSENAKKRHFNWIREVYDYMAPFVSKSPRAAYVNSRDLDI 486
           PF +RAGNLY I Y   WKEQS +AK RH NWIREV ++M PFVSKSPRAAYVN RDLDI
Sbjct: 424 PFSHRAGNLYKIGYAVSWKEQSLDAKNRHINWIREVNEHMTPFVSKSPRAAYVNYRDLDI 483

Query: 487 GTNDKYGKTSYKRASVWGLKYFGNNFNRLVYVKTKVDPYDFFRHEQSIP 534
           GTN+KYGKTSY++ASVWG+KYFGNNFNRLVYVKTKVDP+DFFRHEQSIP
Sbjct: 484 GTNNKYGKTSYEQASVWGMKYFGNNFNRLVYVKTKVDPHDFFRHEQSIP 531

BLAST of Tan0015432 vs. NCBI nr
Match: KAG6583829.1 (Berberine bridge enzyme-like 26, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 831.2 bits (2146), Expect = 4.9e-237
Identity = 406/529 (76.75%), Postives = 463/529 (87.52%), Query Frame = 0

Query: 7   IPLSLV-LIILSPSPLWVAASADIHQDFLHCLSEHYSSDSYSISKVIYTPINSSYSSVLD 66
           IPL+L+ +I+LS SPLW + S  IHQ FLHCLS H+S  +Y ISK++YTPINSSYS VL+
Sbjct: 4   IPLTLIFIIVLSSSPLWASPSLHIHQHFLHCLS-HHSPHTYPISKLVYTPINSSYSYVLN 63

Query: 67  FSIRNLRFSTADTPKPLVIITPLYVSHIQAAIICSKNHGLQIRTRSGGHDFEGLSYVSHL 126
           FSIRNL FS   TPKPL+IITP ++SHIQAA+ICSK H LQIRTRSGGHDFEGLSYV+  
Sbjct: 64  FSIRNLIFSNPQTPKPLLIITPSHISHIQAAVICSKAHDLQIRTRSGGHDFEGLSYVADQ 123

Query: 127 PFIIVDLINLKSIQIDVNKSTAWVQSGATLGELYYTIAEKSRTLAFPAGSCLTIGIGGHF 186
           PFIIVDLINL+SI +DV  +TAWVQSGATLGELYY I EKSR+LAFPAG C T+G+GGHF
Sbjct: 124 PFIIVDLINLRSINVDVQTNTAWVQSGATLGELYYRIGEKSRSLAFPAGICPTVGVGGHF 183

Query: 187 SGGGRGLMTRKYGLAADNVIDATLVDANGKIHDRESMGEDLFWAIRGGGGGSFGIVVAWK 246
           SGGG GLM RKYGLAADNV+DA LVDANG++HDRESMGEDLFWAIRGGGGGSFGIVVAWK
Sbjct: 184 SGGGYGLMLRKYGLAADNVVDAYLVDANGRVHDRESMGEDLFWAIRGGGGGSFGIVVAWK 243

Query: 247 VKLVPVPATVTICSTNRNLEEDAIMLIHRWQYVANKLDENLFLGIRLSGGNISTQQGITN 306
           VKLV VPA VT+CSTNR++E+DAI LIHRWQYVAN+LD++LFLGI L+GGN ST++GITN
Sbjct: 244 VKLVVVPAVVTMCSTNRDVEDDAIKLIHRWQYVANQLDDDLFLGILLTGGNFSTREGITN 303

Query: 307 PTATFVSFFLGKVDELVAIIKTTFPELGLKKKDCVETSWIESTLIAANGIQTVESLEPLL 366
           PTATF S +LGK+DEL+AI+KTTFPELGL KKDCVE+SWI+STLIA+ G+QT E LE LL
Sbjct: 304 PTATFFSLYLGKLDELIAILKTTFPELGLTKKDCVESSWIQSTLIASTGVQTFEPLEALL 363

Query: 367 NRTPSAEGSTKVKSDFIKEPIPKAAIEGLWQRLKAQDVKA-SYQFIPNGGIMSQISESET 426
           NRTP    STK KSD++K+PIP+AAIEG+WQRLKAQD++     FIP GG MSQISESET
Sbjct: 364 NRTPPTIESTKFKSDYVKDPIPEAAIEGIWQRLKAQDIEVPQILFIPYGGRMSQISESET 423

Query: 427 PFPYRAGNLYGIVYFARWKEQSENAKKRHFNWIREVYDYMAPFVSKSPRAAYVNSRDLDI 486
           PF +RAGNLY I Y   WKEQS +AK RH NWIREVY+YMAPFVSKSPRAAYVN RDLDI
Sbjct: 424 PFSHRAGNLYKIGYAVSWKEQSLDAKNRHINWIREVYEYMAPFVSKSPRAAYVNYRDLDI 483

Query: 487 GTNDKYGKTSYKRASVWGLKYFGNNFNRLVYVKTKVDPYDFFRHEQSIP 534
           GTN+KYGKTSY++ASVWG+KYFGNNFNRLVYVKT VDP+DFFRHEQSIP
Sbjct: 484 GTNNKYGKTSYEQASVWGMKYFGNNFNRLVYVKTMVDPHDFFRHEQSIP 531

BLAST of Tan0015432 vs. ExPASy TrEMBL
Match: A0A5D3C1A1 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00110 PE=3 SV=1)

HSP 1 Score: 820.5 bits (2118), Expect = 4.2e-234
Identity = 411/538 (76.39%), Postives = 459/538 (85.32%), Query Frame = 0

Query: 1   MKYSS-LIPLSLVLIILSPSPLWVAASADIHQDFLHCLSEHYSSDSYSISKVIYTPINSS 60
           MKYSS LIP SL+LI++S S  WV+AS   HQ+FL CLSEH SS  Y ISKV++TPINSS
Sbjct: 1   MKYSSPLIPFSLILILVSSSSSWVSASPHKHQNFLQCLSEH-SSKRYPISKVVHTPINSS 60

Query: 61  YSSVLDFSIRNLRFSTADTPKPLVIITPLYVSHIQAAIICSKNHGLQIRTRSGGHDFEGL 120
           YSSVLDFSIRNL FS  +TPKPL+IITP +VSHIQAA+ICSK+HGLQIRTRSGGHDFEGL
Sbjct: 61  YSSVLDFSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120

Query: 121 SYVSHLPFIIVDLINLKSIQIDVNKSTAWVQSGATLGELYYTIAEKSRTLAFPAGSCLTI 180
           SYV++ PFIIVDLINL+SI+IDV  +TAWV+SGATLGELYY I EKSRTLAFP G C T+
Sbjct: 121 SYVAYHPFIIVDLINLRSIKIDVETNTAWVESGATLGELYYRIGEKSRTLAFPGGVCPTV 180

Query: 181 GIGGHFSGGGRGLMTRKYGLAADNVIDATLVDANGKIHDRESMGEDLFWAIRGGGGGSFG 240
           G+GGHFSGGG GLM RK+GLAADNVIDA LVDANGK+HDR+SMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVHDRKSMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPATVTICSTNRNLEEDAIMLIHRWQYVANKLDENLFLGIRLSGGNI-S 300
           IVVAWK+KLV VPATVTICSTNRNL+ D I L+H+WQYVANKLDENLFLGI L+GGNI +
Sbjct: 241 IVVAWKIKLVSVPATVTICSTNRNLKGDGIKLVHQWQYVANKLDENLFLGIMLTGGNITT 300

Query: 301 TQQGITNPTATFVSFFLGKVDELVAIIKTTFPELGLKKKDCVETSWIESTLIAANGIQTV 360
           TQ+GI NP ATF S FLGKVD LVA +   FPELG  K+DCVETSWIESTLIAA G+ TV
Sbjct: 301 TQEGIANPIATFFSLFLGKVDILVATLSAIFPELGFTKEDCVETSWIESTLIAATGVGTV 360

Query: 361 ESLEPLLNRTPSAEGSTKVKSDFIKEPIPKAAIEGLWQRLKAQDVKASYQ-FIPNGGIMS 420
           ESLEPLLNRTPS   + K+KSD+ KEPI  A IEGLWQRLKA+D++ S   FIP GG MS
Sbjct: 361 ESLEPLLNRTPSNLENGKMKSDYFKEPISIATIEGLWQRLKAKDIETSQLIFIPYGGRMS 420

Query: 421 QISESETPFPYRAGNLYGIVYFARWKEQSENAKKRHFNWIREVYDYMAPFVSKSPRAAYV 480
           QISE ETPF +RAG LY I YF  WKEQS  AKK+H +WIR++Y YM PFVSKSPR+AY 
Sbjct: 421 QISEMETPFSHRAGILYKIGYFVAWKEQSLKAKKKHISWIRDIYKYMTPFVSKSPRSAYA 480

Query: 481 NSRDLDIGTNDKYGKTSYKRASVWGLKYFGNNFNRLVYVKTKVDPYDFFRHEQSIPTL 536
           N RDLDIG N KYGKTS ++AS+WGLKYFGNNF RLVYVKTKVDPYDFFRHEQSIPTL
Sbjct: 481 NYRDLDIGANKKYGKTSVRQASIWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIPTL 537

BLAST of Tan0015432 vs. ExPASy TrEMBL
Match: A0A1S3BDZ5 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488631 PE=3 SV=1)

HSP 1 Score: 820.5 bits (2118), Expect = 4.2e-234
Identity = 411/538 (76.39%), Postives = 459/538 (85.32%), Query Frame = 0

Query: 1   MKYSS-LIPLSLVLIILSPSPLWVAASADIHQDFLHCLSEHYSSDSYSISKVIYTPINSS 60
           MKYSS LIP SL+LI++S S  WV+AS   HQ+FL CLSEH SS  Y ISKV++TPINSS
Sbjct: 1   MKYSSPLIPFSLILILVSSSSSWVSASPHKHQNFLQCLSEH-SSKRYPISKVVHTPINSS 60

Query: 61  YSSVLDFSIRNLRFSTADTPKPLVIITPLYVSHIQAAIICSKNHGLQIRTRSGGHDFEGL 120
           YSSVLDFSIRNL FS  +TPKPL+IITP +VSHIQAA+ICSK+HGLQIRTRSGGHDFEGL
Sbjct: 61  YSSVLDFSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120

Query: 121 SYVSHLPFIIVDLINLKSIQIDVNKSTAWVQSGATLGELYYTIAEKSRTLAFPAGSCLTI 180
           SYV++ PFIIVDLINL+SI+IDV  +TAWV+SGATLGELYY I EKSRTLAFP G C T+
Sbjct: 121 SYVAYHPFIIVDLINLRSIKIDVETNTAWVESGATLGELYYRIGEKSRTLAFPGGVCPTV 180

Query: 181 GIGGHFSGGGRGLMTRKYGLAADNVIDATLVDANGKIHDRESMGEDLFWAIRGGGGGSFG 240
           G+GGHFSGGG GLM RK+GLAADNVIDA LVDANGK+HDR+SMGEDLFWAIRGGGGGSFG
Sbjct: 181 GVGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVHDRKSMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPATVTICSTNRNLEEDAIMLIHRWQYVANKLDENLFLGIRLSGGNI-S 300
           IVVAWK+KLV VPATVTICSTNRNL+ D I L+H+WQYVANKLDENLFLGI L+GGNI +
Sbjct: 241 IVVAWKIKLVSVPATVTICSTNRNLKGDGIKLVHQWQYVANKLDENLFLGIMLTGGNITT 300

Query: 301 TQQGITNPTATFVSFFLGKVDELVAIIKTTFPELGLKKKDCVETSWIESTLIAANGIQTV 360
           TQ+GI NP ATF S FLGKVD LVA +   FPELG  K+DCVETSWIESTLIAA G+ TV
Sbjct: 301 TQEGIANPIATFFSLFLGKVDILVATLSAIFPELGFTKEDCVETSWIESTLIAATGVGTV 360

Query: 361 ESLEPLLNRTPSAEGSTKVKSDFIKEPIPKAAIEGLWQRLKAQDVKASYQ-FIPNGGIMS 420
           ESLEPLLNRTPS   + K+KSD+ KEPI  A IEGLWQRLKA+D++ S   FIP GG MS
Sbjct: 361 ESLEPLLNRTPSNLENGKMKSDYFKEPISIATIEGLWQRLKAKDIETSQLIFIPYGGRMS 420

Query: 421 QISESETPFPYRAGNLYGIVYFARWKEQSENAKKRHFNWIREVYDYMAPFVSKSPRAAYV 480
           QISE ETPF +RAG LY I YF  WKEQS  AKK+H +WIR++Y YM PFVSKSPR+AY 
Sbjct: 421 QISEMETPFSHRAGILYKIGYFVAWKEQSLKAKKKHISWIRDIYKYMTPFVSKSPRSAYA 480

Query: 481 NSRDLDIGTNDKYGKTSYKRASVWGLKYFGNNFNRLVYVKTKVDPYDFFRHEQSIPTL 536
           N RDLDIG N KYGKTS ++AS+WGLKYFGNNF RLVYVKTKVDPYDFFRHEQSIPTL
Sbjct: 481 NYRDLDIGANKKYGKTSVRQASIWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIPTL 537

BLAST of Tan0015432 vs. ExPASy TrEMBL
Match: A0A5A7TU57 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00190 PE=3 SV=1)

HSP 1 Score: 819.3 bits (2115), Expect = 9.4e-234
Identity = 406/537 (75.61%), Postives = 462/537 (86.03%), Query Frame = 0

Query: 1   MKYSS-LIPLSLVLIILSPSPLWVAASADIHQDFLHCLSEHYSSDSYSISKVIYTPINSS 60
           MK+SS LIPLSLV I++S + L  +AS   +Q+FL CLSEH SS SY ISKV+YTPINSS
Sbjct: 1   MKFSSTLIPLSLVFIVVSSASLCASASPQKYQNFLQCLSEH-SSKSYPISKVVYTPINSS 60

Query: 61  YSSVLDFSIRNLRFSTADTPKPLVIITPLYVSHIQAAIICSKNHGLQIRTRSGGHDFEGL 120
           YSSVLDFSIRNL FS  +TPKPL+IITP +VSHIQAA+ICSK+HGLQIRTRSGGHDFEGL
Sbjct: 61  YSSVLDFSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120

Query: 121 SYVSHLPFIIVDLINLKSIQIDVNKSTAWVQSGATLGELYYTIAEKSRTLAFPAGSCLTI 180
           SYV++ PFI+VDLINL+S++++V  +TAWV+SGATLGELYY I EKSRTLAFPAG C T+
Sbjct: 121 SYVAYRPFIVVDLINLRSVKVEVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTV 180

Query: 181 GIGGHFSGGGRGLMTRKYGLAADNVIDATLVDANGKIHDRESMGEDLFWAIRGGGGGSFG 240
           GIGGHFSGGG GLM RK+GLAADNVIDA LVDANGK+ D+ESMGEDLFWAIRGGGGGSFG
Sbjct: 181 GIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVLDKESMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPATVTICSTNRNLEEDAIMLIHRWQYVANKLDENLFLGIRLSGGNIST 300
           IVVAWK+KLVPVP TVTICST+RNLEEDAI LIHRWQYV NKLDEN++LGI L+GGN ST
Sbjct: 241 IVVAWKIKLVPVPETVTICSTDRNLEEDAIKLIHRWQYVGNKLDENIYLGIILTGGNAST 300

Query: 301 QQGITNPTATFVSFFLGKVDELVAIIKTTFPELGLKKKDCVETSWIESTLIAANGIQTVE 360
           Q GITNPTA F S FLG+ DELV  + T FPELGL K+DCVE SW+ESTLI   G+Q +E
Sbjct: 301 QAGITNPTARFFSLFLGRADELVTTLSTIFPELGLIKQDCVEASWVESTLIIPIGVQPIE 360

Query: 361 SLEPLLNRTPSAEGSTKVKSDFIKEPIPKAAIEGLWQRLKAQDVKAS-YQFIPNGGIMSQ 420
           +LEPLLNRT +   STK+KSD+I EPIP AAI+ +WQRLK QD++ S   F+P GG MSQ
Sbjct: 361 TLEPLLNRTLTTLDSTKIKSDYITEPIPVAAIKDIWQRLKTQDIETSQLVFVPYGGRMSQ 420

Query: 421 ISESETPFPYRAGNLYGIVYFARWKEQSENAKKRHFNWIREVYDYMAPFVSKSPRAAYVN 480
           ISESETPF +RA  L+ I Y   WK+QS  AKKRH +W+REVY+YMAPFVSKSPRAAY N
Sbjct: 421 ISESETPFSHRARYLFKIGYVVGWKDQSLKAKKRHISWLREVYEYMAPFVSKSPRAAYTN 480

Query: 481 SRDLDIGTNDKYGKTSYKRASVWGLKYFGNNFNRLVYVKTKVDPYDFFRHEQSIPTL 536
            RDLDIG+N+KYGKTSYKRASVWGLKYFGNNF+RLVYVKTKVDPYDFFRHEQSIPTL
Sbjct: 481 YRDLDIGSNNKYGKTSYKRASVWGLKYFGNNFDRLVYVKTKVDPYDFFRHEQSIPTL 536

BLAST of Tan0015432 vs. ExPASy TrEMBL
Match: A0A1S3BDA8 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488637 PE=3 SV=1)

HSP 1 Score: 819.3 bits (2115), Expect = 9.4e-234
Identity = 406/537 (75.61%), Postives = 462/537 (86.03%), Query Frame = 0

Query: 1   MKYSS-LIPLSLVLIILSPSPLWVAASADIHQDFLHCLSEHYSSDSYSISKVIYTPINSS 60
           MK+SS LIPLSLV I++S + L  +AS   +Q+FL CLSEH SS SY ISKV+YTPINSS
Sbjct: 1   MKFSSTLIPLSLVFIVVSSASLCASASPQKYQNFLQCLSEH-SSKSYPISKVVYTPINSS 60

Query: 61  YSSVLDFSIRNLRFSTADTPKPLVIITPLYVSHIQAAIICSKNHGLQIRTRSGGHDFEGL 120
           YSSVLDFSIRNL FS  +TPKPL+IITP +VSHIQAA+ICSK+HGLQIRTRSGGHDFEGL
Sbjct: 61  YSSVLDFSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120

Query: 121 SYVSHLPFIIVDLINLKSIQIDVNKSTAWVQSGATLGELYYTIAEKSRTLAFPAGSCLTI 180
           SYV++ PFI+VDLINL+S++++V  +TAWV+SGATLGELYY I EKSRTLAFPAG C T+
Sbjct: 121 SYVAYRPFIVVDLINLRSVKVEVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTV 180

Query: 181 GIGGHFSGGGRGLMTRKYGLAADNVIDATLVDANGKIHDRESMGEDLFWAIRGGGGGSFG 240
           GIGGHFSGGG GLM RK+GLAADNVIDA LVDANGK+ D+ESMGEDLFWAIRGGGGGSFG
Sbjct: 181 GIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVLDKESMGEDLFWAIRGGGGGSFG 240

Query: 241 IVVAWKVKLVPVPATVTICSTNRNLEEDAIMLIHRWQYVANKLDENLFLGIRLSGGNIST 300
           IVVAWK+KLVPVP TVTICST+RNLEEDAI LIHRWQYV NKLDEN++LGI L+GGN ST
Sbjct: 241 IVVAWKIKLVPVPETVTICSTDRNLEEDAIKLIHRWQYVGNKLDENIYLGIILTGGNAST 300

Query: 301 QQGITNPTATFVSFFLGKVDELVAIIKTTFPELGLKKKDCVETSWIESTLIAANGIQTVE 360
           Q GITNPTA F S FLG+ DELV  + T FPELGL K+DCVE SW+ESTLI   G+Q +E
Sbjct: 301 QAGITNPTARFFSLFLGRADELVTTLSTIFPELGLIKQDCVEASWVESTLIIPIGVQPIE 360

Query: 361 SLEPLLNRTPSAEGSTKVKSDFIKEPIPKAAIEGLWQRLKAQDVKAS-YQFIPNGGIMSQ 420
           +LEPLLNRT +   STK+KSD+I EPIP AAI+ +WQRLK QD++ S   F+P GG MSQ
Sbjct: 361 TLEPLLNRTLTTLDSTKIKSDYITEPIPVAAIKDIWQRLKTQDIETSQLVFVPYGGRMSQ 420

Query: 421 ISESETPFPYRAGNLYGIVYFARWKEQSENAKKRHFNWIREVYDYMAPFVSKSPRAAYVN 480
           ISESETPF +RA  L+ I Y   WK+QS  AKKRH +W+REVY+YMAPFVSKSPRAAY N
Sbjct: 421 ISESETPFSHRARYLFKIGYVVGWKDQSLKAKKRHISWLREVYEYMAPFVSKSPRAAYTN 480

Query: 481 SRDLDIGTNDKYGKTSYKRASVWGLKYFGNNFNRLVYVKTKVDPYDFFRHEQSIPTL 536
            RDLDIG+N+KYGKTSYKRASVWGLKYFGNNF+RLVYVKTKVDPYDFFRHEQSIPTL
Sbjct: 481 YRDLDIGSNNKYGKTSYKRASVWGLKYFGNNFDRLVYVKTKVDPYDFFRHEQSIPTL 536

BLAST of Tan0015432 vs. ExPASy TrEMBL
Match: A0A6J1KHF6 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111495284 PE=3 SV=1)

HSP 1 Score: 817.4 bits (2110), Expect = 3.6e-233
Identity = 398/529 (75.24%), Postives = 456/529 (86.20%), Query Frame = 0

Query: 7   IPLSLV-LIILSPSPLWVAASADIHQDFLHCLSEHYSSDSYSISKVIYTPINSSYSSVLD 66
           IPL+L+ +I+LS SPLW + S   HQ FLHCLS H+S  +Y ISK++YTPINSSYS VL+
Sbjct: 4   IPLTLIFIIVLSSSPLWASPSLHKHQHFLHCLS-HHSPHTYPISKLVYTPINSSYSPVLN 63

Query: 67  FSIRNLRFSTADTPKPLVIITPLYVSHIQAAIICSKNHGLQIRTRSGGHDFEGLSYVSHL 126
           FSIRNL FS   TPKPL+IITP ++SHIQAAI+CSK H LQIRTRSGGHDFEGLSYV++ 
Sbjct: 64  FSIRNLIFSNPQTPKPLLIITPSHISHIQAAIVCSKAHDLQIRTRSGGHDFEGLSYVANQ 123

Query: 127 PFIIVDLINLKSIQIDVNKSTAWVQSGATLGELYYTIAEKSRTLAFPAGSCLTIGIGGHF 186
           PFIIVDLINL+SI +DV  +TAWVQSGATLGELYY I E SR+LAFPAG C T+G+GGHF
Sbjct: 124 PFIIVDLINLRSINVDVQTNTAWVQSGATLGELYYRIGETSRSLAFPAGICPTVGVGGHF 183

Query: 187 SGGGRGLMTRKYGLAADNVIDATLVDANGKIHDRESMGEDLFWAIRGGGGGSFGIVVAWK 246
           SGGG GLM RKYGLAADNV+DA LVDANG++HDRESMGEDLFWAIRGGGGGSFGIVVAWK
Sbjct: 184 SGGGYGLMLRKYGLAADNVVDAYLVDANGRVHDRESMGEDLFWAIRGGGGGSFGIVVAWK 243

Query: 247 VKLVPVPATVTICSTNRNLEEDAIMLIHRWQYVANKLDENLFLGIRLSGGNISTQQGITN 306
           VKLV VPA VT+CSTNR++E+DAI LIHRWQYVAN+LD+NLFLGI L+GGN ST++GITN
Sbjct: 244 VKLVMVPAVVTMCSTNRDVEDDAIKLIHRWQYVANQLDDNLFLGILLTGGNFSTREGITN 303

Query: 307 PTATFVSFFLGKVDELVAIIKTTFPELGLKKKDCVETSWIESTLIAANGIQTVESLEPLL 366
           PTATF S +LGK+DEL+ I+K  F ELGL KKDCVE+SWI+STLIA+ G+QT E LE LL
Sbjct: 304 PTATFFSLYLGKLDELITILKNNFSELGLTKKDCVESSWIQSTLIASTGVQTFEPLEALL 363

Query: 367 NRTPSAEGSTKVKSDFIKEPIPKAAIEGLWQRLKAQDVKA-SYQFIPNGGIMSQISESET 426
           NRTP    STK KSD++K+PIP+A IEG+WQRLKAQD++     FIP GG MSQISESET
Sbjct: 364 NRTPPTIESTKFKSDYVKDPIPEATIEGIWQRLKAQDIEVPQILFIPYGGRMSQISESET 423

Query: 427 PFPYRAGNLYGIVYFARWKEQSENAKKRHFNWIREVYDYMAPFVSKSPRAAYVNSRDLDI 486
           PF +RAGNLY I Y   WKEQS +AK +H NWIREVY+YM PFVSKSPRAAY N RDLDI
Sbjct: 424 PFSHRAGNLYKIGYAVSWKEQSLDAKNKHINWIREVYEYMTPFVSKSPRAAYANYRDLDI 483

Query: 487 GTNDKYGKTSYKRASVWGLKYFGNNFNRLVYVKTKVDPYDFFRHEQSIP 534
           GTN+KYGKTSY++ASVWG+KYFGNNFNRLVYVKTKVDP+DFFRHEQSIP
Sbjct: 484 GTNNKYGKTSYEQASVWGMKYFGNNFNRLVYVKTKVDPHDFFRHEQSIP 531

BLAST of Tan0015432 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 515.8 bits (1327), Expect = 4.3e-146
Identity = 262/532 (49.25%), Postives = 363/532 (68.23%), Query Frame = 0

Query: 3   YSSLIPLSLVLIILSPSPLWVAASADIHQDFLHCLSEHYSSDSYSISKVIYTPINSSYSS 62
           +SS++       +L   P  V+A+      FL CLS    +DS  +SKVI+TP ++S+SS
Sbjct: 7   FSSVLIFFTTSTLLLSIPHPVSANRSNQSSFLQCLSLQL-NDSNIVSKVIHTPNDTSFSS 66

Query: 63  VLDFSIRNLRFSTADTPKPLVIITPLYVSHIQAAIICSKNHGLQIRTRSGGHDFEGLSYV 122
           VL  SI+N RFS  D PKP++I+TP+  S +Q+A+ C++  G+ IRTRSGGHD+EGLSYV
Sbjct: 67  VLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSYV 126

Query: 123 SHLPFIIVDLINLKSIQIDVNKSTAWVQSGATLGELYYTIAEKSRTLAFPAGSCLTIGIG 182
           +H PF+I+DL NL+SI +DV+  + WVQ+GAT+GELYY I +K+RTLAFPAG C T+G+G
Sbjct: 127 THKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGVG 186

Query: 183 GHFSGGGRGLMTRKYGLAADNVIDATLVDANGKIHDRESMGEDLFWAIRGGGGGSFGIVV 242
           GHFSGGG G + RK+GLAAD+VIDA +VDA G+I +R  MGED FWAIRGGGG SF +V+
Sbjct: 187 GHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVVL 246

Query: 243 AWKVKLVPVPATVTICSTNRNLEEDAIMLIHRWQYVANKLDENLFLGIRLSGGNISTQQG 302
           +WK+ L+ VP+TVT+ +  +  E+ A+ +IHRWQ+VA+K+ ++LF+ + L       Q+ 
Sbjct: 247 SWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVML-------QRY 306

Query: 303 ITNPTATFVSFFLGKVDELVAIIKTTFPELGLKKKDCVETSWIESTLIAANGIQTVESLE 362
                A+F   +LG V  L+ ++   FPELGL++ DC E SWIES +  A   +  E   
Sbjct: 307 KNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFA---ELGEEPI 366

Query: 363 PLLNRTPSAEGSTKVKSDFIKEPIPKAAIEGLWQRLKAQDVK-ASYQFIPNGGIMSQISE 422
            +L +   A  + K KSDF++EP+PK AI  LW+RL+  + + A   F P GG MS+I++
Sbjct: 367 NVLTKRTRASLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIAD 426

Query: 423 SETPFPYRAGNLYGIVYFARWKEQSENAKKRHFNWIREVYDYMAPFVSKSPRAAYVNSRD 482
            ETPFP+R GN+Y I Y   W+    + K+++  W+  VYD M+ FV+KSPR AY+N RD
Sbjct: 427 YETPFPHRKGNIYEIQYLNYWR---GDVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRD 486

Query: 483 LDIGTNDKYGKTSYKRASVWGLKYFGNNFNRLVYVKTKVDPYDFFRHEQSIP 534
           LD+G      ++ Y+    WG+KYF NNF RLV VKT VDP DFF  EQSIP
Sbjct: 487 LDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of Tan0015432 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 514.2 bits (1323), Expect = 1.2e-145
Identity = 265/541 (48.98%), Postives = 377/541 (69.69%), Query Frame = 0

Query: 1   MKYSSLIPLSLVLIILSPSPLWVAASADIHQDFLHCLSEHYSSDSYSISKVIYTPINSSY 60
           M++SS +  +++L  L+ SPL V+A    H+DFL CLS +  +D+    KVI+T  +SS+
Sbjct: 1   MEFSSFL-FTILLFSLNISPL-VSAHGSNHEDFLKCLS-YRMNDNTVEPKVIHTSKDSSF 60

Query: 61  SSVLDFSIRNLRFSTADTPKPLVIITPLYVSHIQAAIICSKNHGLQIRTRSGGHDFEGLS 120
            S+LD SI+N RFS ++TPKP+ IITP+  S +Q  I C++ HG+ +RTRS GH +EGLS
Sbjct: 61  FSILDSSIQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLS 120

Query: 121 YVSH-LPFIIVDLINLKSIQIDVNKSTAWVQSGATLGELYYTIAEKSRTLAFPAGSCLTI 180
           Y+++  PF ++DL NL+SI +DV+  T WVQ+GAT GELYY I + +++LAFPAG   T+
Sbjct: 121 YIAYNKPFAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTV 180

Query: 181 GIGGHFSGGGRGLMTRKYGLAADNVIDATLVDANGKIHDRESMGEDLFWAIRGGGGGSFG 240
           G+GG FSGGG G + RKYGLAADN+IDA +VDA+G+I DR++MGED FWAIRGGGG SFG
Sbjct: 181 GVGGQFSGGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFG 240

Query: 241 IVVAWKVKLVPVPATVTICSTNRNLEEDAIMLIHRWQYVANKLDENLFLGIRLSGGNIST 300
           ++++WKVKLV VP+T+T+    +  +++A+ +I +WQY A+K+ ++LF+   L   N + 
Sbjct: 241 VILSWKVKLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTLERSNKNA 300

Query: 301 QQGITNPTATFVSFFLGKVDELVAIIKTTFPELGLKKKDCVETSWIESTLIAANGIQTVE 360
                   A F   ++G V+ L+A+++  FPELGL+K+ C E SWIES L  A+     E
Sbjct: 301 VH------ALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFAD-FPKGE 360

Query: 361 SLEPLLNRTPSAEGSTKVKSDFIKEPIPKAAIEGLWQRLKAQDVKASYQFI-PNGGIMSQ 420
           SL  L NR  ++  S K K DF++EPIP+AAI+ +W+RL+A + +     + P GG MS+
Sbjct: 361 SLGVLTNRERTSL-SFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSE 420

Query: 421 ISESETPFPYRAGNLYGIVYFARWKEQSENAK---KRHFNWIREVYDYMAPFVSKSPRAA 480
           ++E ETPFP+R GNLY I Y A W+E+ +  K    ++  W+  VY++M P+VSKSPR A
Sbjct: 421 MAEYETPFPHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGA 480

Query: 481 YVNSRDLDIGTNDKYGKTSYKRASVWGLKYFGNNFNRLVYVKTKVDPYDFFRHEQSIPTL 537
           YVN +D+D+G      KT Y+    WG+KYF NNF RLV VKT+VDP DFF  EQSIP +
Sbjct: 481 YVNFKDMDLGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIPLV 530

BLAST of Tan0015432 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 506.9 bits (1304), Expect = 2.0e-143
Identity = 259/519 (49.90%), Postives = 355/519 (68.40%), Query Frame = 0

Query: 23  VAASADIHQDFLHCLSEHYSSDSYSISKVIYTPINSSYSSVLDFSIRNLRFSTADTPKPL 82
           ++A    H++FL CLS   + D    S++I+T  + SY S+L+ SI+N RF   +TPKP+
Sbjct: 27  ISAQRTNHENFLKCLSHRINEDD---SRIIHTSKDPSYFSILNSSIQNPRFFVLETPKPV 86

Query: 83  VIITPLYVSHIQAAIICSKNHGLQIRTRSGGHDFEGLSYVS-HLPFIIVDLINLKSIQID 142
            IITP+  + +Q+ I C++ HG+ IRTRSGGHD+EGLSY++   PF+++DL NL+SI +D
Sbjct: 87  SIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFVVIDLRNLRSITLD 146

Query: 143 VNKSTAWVQSGATLGELYYTIAEKSRTLAFPAGSCLTIGIGGHFSGGGRGLMTRKYGLAA 202
           V+  T WVQSGAT+GELYY I + S++LAFPAG   T+GIGG F GGG G + RKYGL+A
Sbjct: 147 VDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGGGYGTLMRKYGLSA 206

Query: 203 DNVIDATLVDANGKIHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICSTN 262
           DNVIDA +VDANG   DR+ MGED FWAIRGGGG SF +V++WK++L+ VP+ VT+    
Sbjct: 207 DNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRLLDVPSVVTVFKVV 266

Query: 263 RNLEEDAIMLIHRWQYVANKLDENLFLGIRLSGGNISTQQGITNPTATFVSFFLGKVDEL 322
           +  E++A+ +I++WQY+A+K+  +LF+   L        Q  T   A+F   +LG V +L
Sbjct: 267 KTSEKEAVSIINKWQYIADKVPNDLFIRAML--------QKETEVYASFPGLYLGPVSDL 326

Query: 323 VAIIKTTFPELGLKKKDCVETSWIESTLIAANGIQTVESLEPLLNRTPSAEGSTKVKSDF 382
           +A++K  FPELGL+  +C E SWIES L    G    ES+E L  R  ++  S K K DF
Sbjct: 327 LALMKDKFPELGLEIGNCREMSWIESVLWFIKG----ESMEILAKRKRTSR-SFKGKDDF 386

Query: 383 IKEPIPKAAIEGLWQRLKAQDVK-ASYQFIPNGGIMSQISESETPFPYRAGNLYGIVYFA 442
           I+EPIPK AI+ LW+R +A + + A     P GG MS+I+++E PFP+R GNLY I Y A
Sbjct: 387 IEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPFPHREGNLYEIQYLA 446

Query: 443 RWKEQSENAK---KRHFNWIREVYDYMAPFVSKSPRAAYVNSRDLDIGTNDKYG-KTSYK 502
            W E+ +  K   +++  W+  VY++M P+VSKSPR AYVN RD+D+G       KT Y+
Sbjct: 447 YWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDIDLGMYLGLNMKTKYE 506

Query: 503 RASVWGLKYFGNNFNRLVYVKTKVDPYDFFRHEQSIPTL 536
            A VWG+KYF NNF+RLV VKT VDP DFF  EQSIP +
Sbjct: 507 EAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIPIM 529

BLAST of Tan0015432 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 502.7 bits (1293), Expect = 3.7e-142
Identity = 251/530 (47.36%), Postives = 366/530 (69.06%), Query Frame = 0

Query: 9   LSLVLIILSPSPLWVAASADIHQDFLHCLSEHYSSDSYSISKVIYTPINSS--YSSVLDF 68
           L LV ++L   PL   +S+ + QDF+ CL ++ S  S+ I+   ++P  ++  +   L+ 
Sbjct: 12  LFLVTLLLISVPL---SSSTLQQDFVKCLVDN-SDVSFPITASFFSPDQNATLFKEELES 71

Query: 69  SIRNLRFSTADTPKPLVIITPLYVSHIQAAIICSKNHGLQIRTRSGGHDFEGLSYVS--H 128
           + +NLR+ T   PKP+ I  PLY +H+QAA++C+K   L +R RSGGHD+EGLS+V+   
Sbjct: 72  TAQNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDE 131

Query: 129 LPFIIVDLINLKSIQIDVNKSTAWVQSGATLGELYYTIAEKSRTLAFPAGSCLTIGIGGH 188
            PF+IVDL  L+ + +D++ ++AW  +GAT+GE+YY I EKS+T  FPAG C ++GIGGH
Sbjct: 132 TPFVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGH 191

Query: 189 FSGGGRGLMTRKYGLAADNVIDATLVDANGKIHDRESMGEDLFWAIRGGGGGSFGIVVAW 248
             GG  G M RK+GL ADNV+DA +VDANG+I DR +MGED+FWAIRGGGGGSFG+++AW
Sbjct: 192 LVGGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAW 251

Query: 249 KVKLVPVPATVTICSTNRNLEEDAIMLIHRWQYVANKLDENLFLGIRLSGGNISTQQGIT 308
           K+KLVPVPATVT+ +  + LE+D   ++++W+ +A+KLD++LF+ + +S  + +T+ G  
Sbjct: 252 KIKLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPGNR 311

Query: 309 NPTATFVSFFLGKVDELVAIIKTTFPELGLKKKDCVETSWIESTLIAANGIQTVESLEPL 368
             + ++ + FLG  + L+ +++ +FPELGL KKDC E SWI+S +  A G     + E L
Sbjct: 312 TISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIA-GFPNSAAPEAL 371

Query: 369 LNRTPSAEGSTKVKSDFIKEPIPKAAIEGLWQRLKAQDVKASYQFIPNGGIMSQISESET 428
           L      +   K KSDF+KEPIP   +EGLW+R   +D   +  + P GG+MS+ISESE 
Sbjct: 372 LAGKSLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLTI-WNPYGGMMSRISESEI 431

Query: 429 PFPYRAGNLYGIVYFARWKEQSENAKKRHFNWIREVYDYMAPFVSKSPRAAYVNSRDLDI 488
           PFP+R G L+ I + + W++  + +++RH  WIRE+Y YM  +VSK+PR AYVN RDLD+
Sbjct: 432 PFPHRNGTLFKIQWLSTWQD-GKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDL 491

Query: 489 GTNDKYGKTSYKRASVWGLKYFGNNFNRLVYVKTKVDPYDFFRHEQSIPT 535
           GTN+  G+T    A  WG KY+  NF RLV +K + DP +FFRHEQS+PT
Sbjct: 492 GTNE--GETD---AREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPT 529

BLAST of Tan0015432 vs. TAIR 10
Match: AT5G44380.1 (FAD-binding Berberine family protein )

HSP 1 Score: 501.1 bits (1289), Expect = 1.1e-141
Identity = 254/519 (48.94%), Postives = 350/519 (67.44%), Query Frame = 0

Query: 24  AASADIHQDFLHCLSEHYSSDSYSISKVIYTPIN--SSYSSVLDFSIRNLRFSTADTPKP 83
           ++SA I   F++C+  + +  S+ + K ++TP    S ++ VL+ + +NL+F     PKP
Sbjct: 29  SSSASIQDQFINCVKRN-THVSFPLEKTLFTPAKNVSLFNQVLESTAQNLQFLAKSMPKP 88

Query: 84  LVIITPLYVSHIQAAIICSKNHGLQIRTRSGGHDFEGLSYVSHL--PFIIVDLINLKSIQ 143
             I  P++ S +QA+IICSK  G+  R RSGGHDFE LSYVS +  PFI++DL  LK I 
Sbjct: 89  GFIFRPIHQSQVQASIICSKKLGIHFRVRSGGHDFEALSYVSRIEKPFILLDLSKLKQIN 148

Query: 144 IDVNKSTAWVQSGATLGELYYTIAEKSRTLAFPAGSCLTIGIGGHFSGGGRGLMTRKYGL 203
           +D+  ++AWVQ GATLGELYY IAEKS+   FPAG C ++GIGG+ +GGG G + RKYGL
Sbjct: 149 VDIESNSAWVQPGATLGELYYRIAEKSKIHGFPAGLCTSVGIGGYMTGGGYGTLMRKYGL 208

Query: 204 AADNVIDATLVDANGKIHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICS 263
           A DNV+D  +VDANGK+ DR +MGEDLFWAIRGGGG SFGIV+AWK+KLVPVP TVT+ +
Sbjct: 209 AGDNVLDVKMVDANGKLLDRAAMGEDLFWAIRGGGGASFGIVLAWKIKLVPVPKTVTVFT 268

Query: 264 TNRNLEEDA-IMLIHRWQYVANKLDENLFLGIRL-SGGNISTQQGITNPTATFVSFFLGK 323
             + LE+DA +  I +WQ +++K+ E + + + L + GN     G    T T++  FLG+
Sbjct: 269 VTKTLEQDARLKTISKWQQISSKIIEEIHIRVVLRAAGN----DGNKTVTMTYLGQFLGE 328

Query: 324 VDELVAIIKTTFPELGLKKKDCVETSWIESTLIAANGIQTVESLEPLLN-RTPSAEGSTK 383
              L+ +++  FPELGL +KDC E SWIE+ L    G  T   +E LL  ++P  +   K
Sbjct: 329 KGTLLKVMEKAFPELGLTQKDCTEMSWIEAALF-HGGFPTGSPIEILLQLKSPLGKDYFK 388

Query: 384 VKSDFIKEPIPKAAIEGLWQRLKAQDVKASYQFIPNGGIMSQISESETPFPYRAGNLYGI 443
             SDF+KEPIP   ++G+++RL  +       + P GG+MS+I ES  PFP+R G L+ I
Sbjct: 389 ATSDFVKEPIPVIGLKGIFKRL-IEGNTTFLNWTPYGGMMSKIPESAIPFPHRNGTLFKI 448

Query: 444 VYFARWKEQSENAKKRHFNWIREVYDYMAPFVSKSPRAAYVNSRDLDIGTNDKYGKTSYK 503
           +Y+A W E ++    R  NWI+E+Y+YMAP+VS +PR AYVN RDLD G N    K ++ 
Sbjct: 449 LYYANWLE-NDKTSSRKINWIKEIYNYMAPYVSSNPRQAYVNYRDLDFGQNKNNAKVNFI 508

Query: 504 RASVWGLKYFGNNFNRLVYVKTKVDPYDFFRHEQSIPTL 536
            A +WG KYF  NF+RLV +KTKVDP +FFRHEQSIP +
Sbjct: 509 EAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQSIPPM 539