Tan0014447 (gene) Snake gourd v1

Overview


Name	Tan0014447
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Pectate lyase
Location	LG01: 54984656 .. 54986243 (-)
RNA-Seq Expression	Tan0014447
Synteny	Tan0014447

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCGCTTTTAGAACCTTGTTGGTCGTCTGTTCGCTGTTGGTGTTTGCGGTGGCAAATACTGATGTGAATGAGGTTGATGAATACTGGAAGACTCGTGCCCGCGAAGCTCAAAAGGCCGCTCAAGATGCCTACATTCCCAACCCTGAAACTATTACCGATGACTTCAATGCCGCTGTGGGCCGGTAAGTTTCTAATGGATTTAGACTTTGCTCTGATACCATATTAAATTAATTACCTTGTGTCATACAGGGAGATGACGGGCAGCAACAGTACGAGAAGGAGCCTGAAGAAATACAAGGGTCCATGCTTGGCGACGAACCCCATAGACAGATGCTGGAGATGCGATCCAAATTGGGCTAACAACAGAAAGAAGCTGGCGGAATGTCCCTTGGGGTTTGGCCACCAAACCATCGGCGGAAAGAGAGGACCCATCTACGTTGTGACAGACGGCTCCGACGATGACATTGTGAACCCAAAGCCCGGAACCCTACGCCACGCCGTTATTCAAAAAGGCCCACTTTGGATCATCTTCGGTCGTAGCATGATCATCAGGCTCACCCAAGAACTCATGATTTCAAGCAATAAGACCATCGACGCTCGTGGCGCCAATGTCCACATTGCCTTCGGCGCTGGACTCACCATTCAGTTCGTTAACAATGTCATCATTCATGGCCTTCACATTCACGACATTGTCTCCAAATCTGGTGGAATGATTAGAGATTCAGTTGATCATTACGGCTTCCGAACCCAAAGCGACGGGGATGGCATCTCCATCTTTGGGTCTTCTCATGTTTGGATTGACCATGTTTCTATGTCCAATTGCCAAGATGGACTGATTGATGCCATCATGGGTTCCACTGCCATCACAATCTCCAACTCCCATTTCACTCATCACAATGAGGTATCAACTCAATTAGACTTTGATGAGAGAGTCATTAATAAAAATATTAATTATTGTGTGTATATATATATATATATGTAGGTGATGTTGTTCGGAGCGAGTGATAGTTACTCCGAGGACTCTGTTATGCAAGTAACGTTGGCATTCAACCACTTTGGAAGGGGATTGGTGCAAAGGATGCCAAGGTGCAGATGGGGTTTCTTCCATGTCGTTAATAATGATTACACTCATTGGCTTATGTATGCCATTGGCGGCAGCCAGCATCCCACCATTGTTAGCCAAGGAAATCGTTTCATTGCTCCTCCTAATCCTAATGCAAAAAATGTAAATATTTAAATTCACTCGTCAAGTTTAAGATTTCCAGTCTAGTGAATTAGGAATTATTTATTAAAATTTGCATTCTTGATCAAATTAGGTCATATGAAATTAATTGTGGGGGAAATAATGGTGCAGGTAACAAAGAGAGAATATGCAATGGAGAGCGAATGGAAGAACTGGTCATGGAGATCAGAGGGAGATTTGATGATGAATGGGGCAGTCTTTGTTGCATCAGGCGATCAAACAAAAGGGAAGAAGAAGAACTTATCAAGATATGAGATGATTACGTTCAAGCCAGGAAGTTACGTGAGAAGACTTACACGCTTCTCTGGCACACTTGAATGCGTCGTAGGCAAACCATGCTAG

mRNA sequence

ATGGCCGCTTTTAGAACCTTGTTGGTCGTCTGTTCGCTGTTGGTGTTTGCGGTGGCAAATACTGATGTGAATGAGGTTGATGAATACTGGAAGACTCGTGCCCGCGAAGCTCAAAAGGCCGCTCAAGATGCCTACATTCCCAACCCTGAAACTATTACCGATGACTTCAATGCCGCTGTGGGCCGGGAGATGACGGGCAGCAACAGTACGAGAAGGAGCCTGAAGAAATACAAGGGTCCATGCTTGGCGACGAACCCCATAGACAGATGCTGGAGATGCGATCCAAATTGGGCTAACAACAGAAAGAAGCTGGCGGAATGTCCCTTGGGGTTTGGCCACCAAACCATCGGCGGAAAGAGAGGACCCATCTACGTTGTGACAGACGGCTCCGACGATGACATTGTGAACCCAAAGCCCGGAACCCTACGCCACGCCGTTATTCAAAAAGGCCCACTTTGGATCATCTTCGGTCGTAGCATGATCATCAGGCTCACCCAAGAACTCATGATTTCAAGCAATAAGACCATCGACGCTCGTGGCGCCAATGTCCACATTGCCTTCGGCGCTGGACTCACCATTCAGTTCGTTAACAATGTCATCATTCATGGCCTTCACATTCACGACATTGTCTCCAAATCTGGTGGAATGATTAGAGATTCAGTTGATCATTACGGCTTCCGAACCCAAAGCGACGGGGATGGCATCTCCATCTTTGGGTCTTCTCATGTTTGGATTGACCATGTTTCTATGTCCAATTGCCAAGATGGACTGATTGATGCCATCATGGGTTCCACTGCCATCACAATCTCCAACTCCCATTTCACTCATCACAATGAGGTGATGTTGTTCGGAGCGAGTGATAGTTACTCCGAGGACTCTGTTATGCAAGTAACGTTGGCATTCAACCACTTTGGAAGGGGATTGGTGCAAAGGATGCCAAGGTGCAGATGGGGTTTCTTCCATGTCGTTAATAATGATTACACTCATTGGCTTATGTATGCCATTGGCGGCAGCCAGCATCCCACCATTGTTAGCCAAGGAAATCGTTTCATTGCTCCTCCTAATCCTAATGCAAAAAATGTAACAAAGAGAGAATATGCAATGGAGAGCGAATGGAAGAACTGGTCATGGAGATCAGAGGGAGATTTGATGATGAATGGGGCAGTCTTTGTTGCATCAGGCGATCAAACAAAAGGGAAGAAGAAGAACTTATCAAGATATGAGATGATTACGTTCAAGCCAGGAAGTTACGTGAGAAGACTTACACGCTTCTCTGGCACACTTGAATGCGTCGTAGGCAAACCATGCTAG

Coding sequence (CDS)

ATGGCCGCTTTTAGAACCTTGTTGGTCGTCTGTTCGCTGTTGGTGTTTGCGGTGGCAAATACTGATGTGAATGAGGTTGATGAATACTGGAAGACTCGTGCCCGCGAAGCTCAAAAGGCCGCTCAAGATGCCTACATTCCCAACCCTGAAACTATTACCGATGACTTCAATGCCGCTGTGGGCCGGGAGATGACGGGCAGCAACAGTACGAGAAGGAGCCTGAAGAAATACAAGGGTCCATGCTTGGCGACGAACCCCATAGACAGATGCTGGAGATGCGATCCAAATTGGGCTAACAACAGAAAGAAGCTGGCGGAATGTCCCTTGGGGTTTGGCCACCAAACCATCGGCGGAAAGAGAGGACCCATCTACGTTGTGACAGACGGCTCCGACGATGACATTGTGAACCCAAAGCCCGGAACCCTACGCCACGCCGTTATTCAAAAAGGCCCACTTTGGATCATCTTCGGTCGTAGCATGATCATCAGGCTCACCCAAGAACTCATGATTTCAAGCAATAAGACCATCGACGCTCGTGGCGCCAATGTCCACATTGCCTTCGGCGCTGGACTCACCATTCAGTTCGTTAACAATGTCATCATTCATGGCCTTCACATTCACGACATTGTCTCCAAATCTGGTGGAATGATTAGAGATTCAGTTGATCATTACGGCTTCCGAACCCAAAGCGACGGGGATGGCATCTCCATCTTTGGGTCTTCTCATGTTTGGATTGACCATGTTTCTATGTCCAATTGCCAAGATGGACTGATTGATGCCATCATGGGTTCCACTGCCATCACAATCTCCAACTCCCATTTCACTCATCACAATGAGGTGATGTTGTTCGGAGCGAGTGATAGTTACTCCGAGGACTCTGTTATGCAAGTAACGTTGGCATTCAACCACTTTGGAAGGGGATTGGTGCAAAGGATGCCAAGGTGCAGATGGGGTTTCTTCCATGTCGTTAATAATGATTACACTCATTGGCTTATGTATGCCATTGGCGGCAGCCAGCATCCCACCATTGTTAGCCAAGGAAATCGTTTCATTGCTCCTCCTAATCCTAATGCAAAAAATGTAACAAAGAGAGAATATGCAATGGAGAGCGAATGGAAGAACTGGTCATGGAGATCAGAGGGAGATTTGATGATGAATGGGGCAGTCTTTGTTGCATCAGGCGATCAAACAAAAGGGAAGAAGAAGAACTTATCAAGATATGAGATGATTACGTTCAAGCCAGGAAGTTACGTGAGAAGACTTACACGCTTCTCTGGCACACTTGAATGCGTCGTAGGCAAACCATGCTAG

Protein sequence

MAAFRTLLVVCSLLVFAVANTDVNEVDEYWKTRAREAQKAAQDAYIPNPETITDDFNAAVGREMTGSNSTRRSLKKYKGPCLATNPIDRCWRCDPNWANNRKKLAECPLGFGHQTIGGKRGPIYVVTDGSDDDIVNPKPGTLRHAVIQKGPLWIIFGRSMIIRLTQELMISSNKTIDARGANVHIAFGAGLTIQFVNNVIIHGLHIHDIVSKSGGMIRDSVDHYGFRTQSDGDGISIFGSSHVWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNEVMLFGASDSYSEDSVMQVTLAFNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKNVTKREYAMESEWKNWSWRSEGDLMMNGAVFVASGDQTKGKKKNLSRYEMITFKPGSYVRRLTRFSGTLECVVGKPC

Homology

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BLAST of Tan0014447 vs. ExPASy Swiss-Prot
Match: P15722 (Probable pectate lyase P59 OS=Solanum lycopersicum OX=4081 GN=LAT59 PE=2 SV=1)

HSP 1 Score: 570.1 bits (1468), Expect = 2.2e-161
Identity = 276/425 (64.94%), Postives = 323/425 (76.00%), Query Frame = 0


Query: 23  VNEVDEYWKTRAREAQKAAQDAYIPNPETITDDFNAAVGREM--------TGSNSTRRSL 82
           +   DE W+ RA EA++ A++ Y P+PE +T  FN  +   M        T +NSTRR L
Sbjct: 28  IGHYDEVWRRRAEEAKEYARNIYEPHPENVTLAFNQKLRDTMKELKKVKGTHNNSTRRGL 87

Query: 83  --KKYKGPCLATNPIDRCWRCDPNWANNRKKLAECPLGFGHQTIGGKRGPIYVVTDGSDD 142
             KKY GPC+ TNPID+CWRCDPNWA+NRKKLA+C +GFG + IGGK G  YVVTD S D
Sbjct: 88  GTKKYTGPCMVTNPIDKCWRCDPNWADNRKKLADCAMGFGSKAIGGKDGEFYVVTDNS-D 147

Query: 143 DIVNPKPGTLRHAVIQKGPLWIIFGRSMIIRLTQELMISSNKTIDARGANVHIAFGAGLT 202
           D  +PKPGTLRHAVIQK PLWIIF R M IRL QE+++ S+KTIDARG NVHI  GAG+T
Sbjct: 148 DYNDPKPGTLRHAVIQKEPLWIIFKRGMNIRLHQEMIMQSDKTIDARGVNVHITKGAGIT 207

Query: 203 IQFVNNVIIHGLHIHDIVSKSGGMIRDSVDHYGFRTQSDGDGISIFGSSHVWIDHVSMSN 262
           +Q++ NVIIHGLHIHDIV  +GGM+RD+VDH G RT+SDGDGISIFG+S++WIDHVSM  
Sbjct: 208 LQYIKNVIIHGLHIHDIVEGNGGMVRDAVDHIGIRTKSDGDGISIFGASYIWIDHVSMQR 267

Query: 263 CQDGLIDAIMGSTAITISNSHFTHHNEVMLFGASDSYSEDSVMQVTLAFNHFGRGLVQRM 322
           C DGLIDA+ GST ITISN HFT HNEVMLFGASDS S D VMQ+TLAFNHFG+ L+QRM
Sbjct: 268 CYDGLIDAVEGSTGITISNGHFTDHNEVMLFGASDSSSIDQVMQITLAFNHFGKRLIQRM 327

Query: 323 PRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKNVTKREYAMESEW 382
           PRCRWG+ HVVNNDYTHW MYAIGGS HPTI+ QGNRFIAPP+   K VTKREY  ES W
Sbjct: 328 PRCRWGYIHVVNNDYTHWNMYAIGGSMHPTIIHQGNRFIAPPDIFKKQVTKREYNPESVW 387

Query: 383 KNWSWRSEGDLMMNGAVFVASGD-QTKGKKKNLSRYEMITFKPGSYVRRLTRFSGTLECV 437
             W+WRSEG+L MNGA F  SGD +   K K+L  Y+ I+  P   V  +TRF+G L C 
Sbjct: 388 MQWTWRSEGNLFMNGAYFTESGDPEWSSKHKDL--YDGISAAPAEDVTWMTRFAGVLGCK 447

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BLAST of Tan0014447 vs. ExPASy Swiss-Prot
Match: P40973 (Pectate lyase OS=Lilium longiflorum OX=4690 PE=2 SV=1)

HSP 1 Score: 557.0 bits (1434), Expect = 1.9e-157
Identity = 268/429 (62.47%), Postives = 330/429 (76.92%), Query Frame = 0


Query: 12  SLLVF---AVANTDVNEVDEYWKTRAREAQKAAQDAYIPNPETITDDFNAAVGREMTGSN 71
           SLL F   A+++ ++ E DEYW+ +++ AQ  A+ AY P+PE +T+ FN AV     G N
Sbjct: 10  SLLFFASAALSSANIAEFDEYWQKKSKVAQAKAKKAYTPHPEEVTNHFNKAVHSSFEG-N 69

Query: 72  STRRSLKKYK-GPCLATNPIDRCWRCDPNWANNRKKLAECPLGFGHQTIGGKRGPIYVVT 131
           STRR+L+  K G CLATNPIDRCWRC  NW+ NRK L +C  GFG +T GG  G IYVVT
Sbjct: 70  STRRNLRTNKLGQCLATNPIDRCWRCKKNWSANRKDLVKCVKGFGRKTTGGAAGEIYVVT 129

Query: 132 DGSDDDIVNPKPGTLRHAVIQKGPLWIIFGRSMIIRLTQELMISSNKTIDARGANVHIAF 191
           D SDD + +PK GTLR  VIQ  PLWIIFG+SM+IRL QEL+I+++KTID RGANV IA 
Sbjct: 130 DPSDDSLTDPKFGTLRWGVIQDRPLWIIFGKSMVIRLKQELIINNDKTIDGRGANVQIAG 189

Query: 192 GAGLTIQFVNNVIIHGLHIHDIVSKSGGMIRDSVDHYGFRTQSDGDGISIFGSSHVWIDH 251
           GA LT+QFV+NVIIHG+HIHDI    GG+IRDS  H G RT+SDGDGISI GSS++WIDH
Sbjct: 190 GAQLTVQFVHNVIIHGIHIHDIKPGEGGLIRDSEKHSGIRTRSDGDGISIIGSSNIWIDH 249

Query: 252 VSMSNCQDGLIDAIMGSTAITISNSHFTHHNEVMLFGASDSYSEDSVMQVTLAFNHFGRG 311
           VS++ C DGLID I+GSTAITISN H T H++VML GASD+Y++D +MQVT+AFNHFGRG
Sbjct: 250 VSLARCSDGLIDVILGSTAITISNCHLTEHDDVMLLGASDTYTQDEIMQVTVAFNHFGRG 309

Query: 312 LVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKNVTKREYA 371
           LVQRMPRCR+GF HVVNNDYTHW+MYA+GGSQHPTI+SQGNR+IAP    AK VTKR+YA
Sbjct: 310 LVQRMPRCRYGFVHVVNNDYTHWIMYAVGGSQHPTIISQGNRYIAPHIEAAKEVTKRDYA 369

Query: 372 MESEWKNWSWRSEGDLMMNGAVFVASGDQTKGKKKNLSRYEMITFKPGSYVRRLTRFSGT 431
             +EW  W+W+S+GDL ++GA FV SG   + K    S+ ++I  KPG++V+RLTRFSG 
Sbjct: 370 EPAEWSKWTWKSQGDLFVSGAFFVESGGPFENK---YSKKDLIKAKPGTFVQRLTRFSGA 429

Query: 432 LECVVGKPC 437
           L C     C
Sbjct: 430 LNCKENMEC 434

[Close]

BLAST of Tan0014447 vs. ExPASy Swiss-Prot
Match: Q9SRH4 (Probable pectate lyase 7 OS=Arabidopsis thaliana OX=3702 GN=At3g01270 PE=2 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 1.0e-155
Identity = 274/471 (58.17%), Postives = 340/471 (72.19%), Query Frame = 0


Query: 4   FRTLLVVC-SLLVFAVANTDVNEVDEYWKTRAREAQKAAQDAYIPNPETITDDFN----- 63
           F+ + V+C + L+  +     +E DEYW  +A EA+K    AY P+P  I D F+     
Sbjct: 7   FKLVCVICIASLIPTIRANVADETDEYWVNKANEARKHTLMAYHPDPYEIVDHFHERHYD 66

Query: 64  -----------AAVGRE--------MTGSNSTRRSL------------KKYKGPCLATNP 123
                       AV  E         + +NSTRRSL             K  GPC A+NP
Sbjct: 67  NSTDVEGTEEEKAVASEEEDVIEMISSPTNSTRRSLTGRGKGKGKGKWSKLTGPCTASNP 126

Query: 124 IDRCWRCDPNWANNRKKLAECPLGFGHQTIGGKRGPIYVVTDGSDDDIVNPKPGTLRHAV 183
           ID+CWRC P+WA  RKKL  C  GFG++T GGKRG IYVVT   DDD+VNP+PGTLRHAV
Sbjct: 127 IDKCWRCQPDWARRRKKLVHCVRGFGYRTTGGKRGRIYVVTSPRDDDMVNPRPGTLRHAV 186

Query: 184 IQKGPLWIIFGRSMIIRLTQELMISSNKTIDARGANVHIAFGAGLTIQFVNNVIIHGLHI 243
           IQK PLWI+F   M IRL+QELMI+S+KTIDARGANVHIA+GAG+T+Q+V+N+IIHGLH+
Sbjct: 187 IQKEPLWIVFKHDMSIRLSQELMITSDKTIDARGANVHIAYGAGITMQYVHNIIIHGLHV 246

Query: 244 HDIVSKSGGMIRDSVDHYGFRTQSDGDGISIFGSSHVWIDHVSMSNCQDGLIDAIMGSTA 303
           H IV  SGG+IRDS++H+G R ++DGDGISIFG++++W+DH+SMS CQDGLIDAIMGSTA
Sbjct: 247 HHIVKSSGGLIRDSINHFGHRGEADGDGISIFGATNIWLDHISMSKCQDGLIDAIMGSTA 306

Query: 304 ITISNSHFTHHNEVMLFGASDSYSEDSVMQVTLAFNHFGRGLVQRMPRCRWGFFHVVNND 363
           ITISNSHFTHHN+VML GA ++  +D  MQVT+A+NHFG+GLVQRMPR RWGF HVVNND
Sbjct: 307 ITISNSHFTHHNDVMLLGAQNNNMDDKKMQVTVAYNHFGKGLVQRMPRVRWGFVHVVNND 366

Query: 364 YTHWLMYAIGGSQHPTIVSQGNRFIAPPN-PNAKNVTKREYAMESEWKNWSWRSEGDLMM 423
           YTHW +YAIGGSQ PTI+S GNRFIAPP+  + + VTKR+YA ESEWKNW+WRSE D+ M
Sbjct: 367 YTHWELYAIGGSQGPTILSHGNRFIAPPHKQHYREVTKRDYASESEWKNWNWRSEKDVFM 426

Query: 424 NGAVFVASGDQTKGKKKNLSRYEMITFKPGSYVRRLTRFSGTLECVVGKPC 437
           N A F  SG+     K + SR +MI  K G  V +LT+++G L+C VGK C
Sbjct: 427 NNAYFRQSGN--PHFKCSHSRQQMIKPKNGMAVSKLTKYAGALDCRVGKAC 475

[Close]

BLAST of Tan0014447 vs. ExPASy Swiss-Prot
Match: Q9LFP5 (Probable pectate lyase 19 OS=Arabidopsis thaliana OX=3702 GN=At5g15110 PE=2 SV=1)

HSP 1 Score: 547.0 bits (1408), Expect = 2.0e-154
Identity = 272/467 (58.24%), Postives = 332/467 (71.09%), Query Frame = 0


Query: 5   RTLLVVCSLLVFAVANTDVNEVDEYWKTRAREAQKAAQDAYIPNPETITDDFN------- 64
           + +   C  ++      +++E+DEYW  RA EA++    AY  +P  I D F+       
Sbjct: 8   KLMFTFCIAVLIPTIRGNISELDEYWSQRADEAREFTLQAYHSDPYEIVDHFHERHYDNS 67

Query: 65  -----------------AAVGREMTGS--NSTRRSLK--------KYKGPCLATNPIDRC 124
                                 EM GS  NSTRRSL+        K KGPC A+NPID+C
Sbjct: 68  TDVTTPEEDGDAKPEEEEKEFIEMLGSSTNSTRRSLRGKGKGKWSKLKGPCTASNPIDKC 127

Query: 125 WRCDPNWANNRKKLAECPLGFGHQTIGGKRGPIYVVTDGSDDDIVNPKPGTLRHAVIQKG 184
           WRC  +WA  RKKL  C  GFGH+T GGKRG IYVVT   D+D+VNPKPGTLRHAVIQK 
Sbjct: 128 WRCRSDWAKRRKKLTRCVRGFGHRTTGGKRGRIYVVTSNLDEDMVNPKPGTLRHAVIQKE 187

Query: 185 PLWIIFGRSMIIRLTQELMISSNKTIDARGANVHIAFGAGLTIQFVNNVIIHGLHIHDIV 244
           PLWIIF   M IRL QEL+I+S+KTIDARGANVH+A GAG+T+QFV NVIIHGLHIH I 
Sbjct: 188 PLWIIFKNDMSIRLNQELLINSHKTIDARGANVHVAHGAGITMQFVKNVIIHGLHIHHIS 247

Query: 245 SKSGGMIRDSVDHYGFRTQSDGDGISIFGSSHVWIDHVSMSNCQDGLIDAIMGSTAITIS 304
             SGGMIRDSVDH+G RT++DGDG+SI+GSS++W+DH+SMS CQDGLIDAI+GST ITIS
Sbjct: 248 ESSGGMIRDSVDHFGMRTRADGDGLSIYGSSNIWLDHISMSKCQDGLIDAIVGSTGITIS 307

Query: 305 NSHFTHHNEVMLFGASDSYSEDSVMQVTLAFNHFGRGLVQRMPRCRWGFFHVVNNDYTHW 364
           NSHFTHHN+VML GA ++   D  MQVT+A+NHFG+GLVQRMPR RWGF HVVNNDYTHW
Sbjct: 308 NSHFTHHNDVMLLGAQNTNEADKHMQVTVAYNHFGKGLVQRMPRIRWGFVHVVNNDYTHW 367

Query: 365 LMYAIGGSQHPTIVSQGNRFIAPPN-PNAKNVTKREYAMESEWKNWSWRSEGDLMMNGAV 424
            +YAIGGSQ PTI+S GNRFIAPP+ P+ + VTKR+YA E EWK+W+WRS+ D+ MNGA 
Sbjct: 368 ELYAIGGSQGPTILSHGNRFIAPPHKPHYREVTKRDYASEDEWKHWNWRSDKDVFMNGAY 427

Query: 425 FVASGDQTKGKKKNLSRYEMITFKPGSYVRRLTRFSGTLECVVGKPC 437
           F  SG+     K   +R +MI  K G  V +LT+++G L+C VG+ C
Sbjct: 428 FRQSGNPQ--YKCAHTRQQMIKPKNGLAVSKLTKYAGALDCRVGRRC 472

[Close]

BLAST of Tan0014447 vs. ExPASy Swiss-Prot
Match: Q9M9S2 (Probable pectate lyase 3 OS=Arabidopsis thaliana OX=3702 GN=AT59 PE=2 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 7.3e-149
Identity = 258/425 (60.71%), Postives = 308/425 (72.47%), Query Frame = 0


Query: 27  DEYWKTRAREAQKAAQDAYIPNPETITDDFNAAVGREMTGS---NSTRRSL--------- 86
           D YW  R  +A K    +Y P+P  +T+ FN  V   +  S   N TRR L         
Sbjct: 35  DSYWTQRQSDALKQTIGSYDPHPLNVTNHFNYHVNIAVDASESRNDTRRELTQVRSGRKT 94

Query: 87  KKYKGPCLATNPIDRCWRCDPNWANNRKKLAECPLGFGHQTIGGKRGPIYVVTDGSDDDI 146
            K  G CLA NPID CWRCD NWANNRKKLA+C LGFG +T GGK GPIYVV D SD+D+
Sbjct: 95  HKSSGKCLAYNPIDNCWRCDRNWANNRKKLADCVLGFGRRTTGGKDGPIYVVKDASDNDL 154

Query: 147 VNPKPGTLRHAVIQKGPLWIIFGRSMIIRLTQELMISSNKTIDARGANVHIAFGAGLTIQ 206
           +NPKPGTLRHAV + GPLWIIF RSMII+L QELMI+S+KTID RGA V+I  GAGLT+Q
Sbjct: 155 INPKPGTLRHAVTRDGPLWIIFARSMIIKLQQELMITSDKTIDGRGARVYIMEGAGLTLQ 214

Query: 207 FVNNVIIHGLHIHDIVSKSGGMIRDSVDHYGFRTQSDGDGISIFGSSHVWIDHVSMSNCQ 266
           FVNNVIIH +++  IV  +GG+IRDS  H G RT+SDGDGIS+FG++++WIDHVSM+ C 
Sbjct: 215 FVNNVIIHNIYVKHIVPGNGGLIRDSEAHIGLRTKSDGDGISLFGATNIWIDHVSMTRCA 274

Query: 267 DGLIDAIMGSTAITISNSHFTHHNEVMLFGASDSYSEDSVMQVTLAFNHFGRGLVQRMPR 326
           DG+IDAI GSTA+TISNSHFT H EVMLFGA D +  D  MQ+T+AFNHFG+ L QRMPR
Sbjct: 275 DGMIDAIDGSTAVTISNSHFTDHQEVMLFGARDEHVIDKKMQITVAFNHFGKRLEQRMPR 334

Query: 327 CRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKNVTKREYAMESEWKN 386
           CR+G  HVVNNDYTHW MYAIGG+ +PTI+SQGNRFIAPPN  AK +TKREY    EWK+
Sbjct: 335 CRYGTIHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIAPPNEEAKQITKREYTPYGEWKS 394

Query: 387 WSWRSEGDLMMNGAVFVASGDQ---TKGKKKNLSRYEMITFKPGSYVRRLTRFSGTLECV 437
           W+W+SEGD  +NGA FV SG     +   K  L     I  KPG+ VR+LT  +G L C 
Sbjct: 395 WNWQSEGDYFLNGAYFVQSGKANAWSSKPKTPLPNKFTIRPKPGTMVRKLTMDAGVLGCK 454

[Close]

BLAST of Tan0014447 vs. NCBI nr
Match: XP_008456459.1 (PREDICTED: pectate lyase-like [Cucumis melo])

HSP 1 Score: 800.4 bits (2066), Expect = 7.5e-228
Identity = 380/436 (87.16%), Postives = 402/436 (92.20%), Query Frame = 0


Query: 1   MAAFRTLLVVCSLLVFAVANTDVNEVDEYWKTRAREAQKAAQDAYIPNPETITDDFNAAV 60
           MA F TL  VCSLL F VAN +V+E DEYW TRA EA+K A+ A+ PNPE +TD+ NAAV
Sbjct: 1   MAGFSTLF-VCSLLFFVVANANVDE-DEYWNTRATEAKKVAKSAFNPNPEIVTDNLNAAV 60

Query: 61  GREMTGSNSTRRSLKKYKGPCLATNPIDRCWRCDPNWANNRKKLAECPLGFGHQTIGGKR 120
            R M G NSTRRSLKKY+GPCLATNPIDRCWRCD NWA NRKKLAECPLGFGHQTIGGKR
Sbjct: 61  SRAMVGRNSTRRSLKKYRGPCLATNPIDRCWRCDRNWAKNRKKLAECPLGFGHQTIGGKR 120

Query: 121 GPIYVVTDGSDDDIVNPKPGTLRHAVIQKGPLWIIFGRSMIIRLTQELMISSNKTIDARG 180
           GPIYVVTD SDDD+VNPKPGTLR+ VIQKGPLWIIFGRSMIIRL+QELMISS+KTIDARG
Sbjct: 121 GPIYVVTDASDDDLVNPKPGTLRYGVIQKGPLWIIFGRSMIIRLSQELMISSDKTIDARG 180

Query: 181 ANVHIAFGAGLTIQFVNNVIIHGLHIHDIVSKSGGMIRDSVDHYGFRTQSDGDGISIFGS 240
           ANVHIAFGAGLTIQFVNNVIIH LHIHDI+SKSGGMIRDSVDHYGFRTQSDGDGISIFGS
Sbjct: 181 ANVHIAFGAGLTIQFVNNVIIHNLHIHDIISKSGGMIRDSVDHYGFRTQSDGDGISIFGS 240

Query: 241 SHVWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNEVMLFGASDSYSEDSVMQVTLA 300
           SHVWIDHVSMSNC+DGLIDAIMGSTAITISN HFTHHNEVMLFGASDSY+ED +MQVTLA
Sbjct: 241 SHVWIDHVSMSNCEDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYAEDEIMQVTLA 300

Query: 301 FNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKN 360
           FNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAK 
Sbjct: 301 FNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKQ 360

Query: 361 VTKREYAMESEWKNWSWRSEGDLMMNGAVFVASGDQTKGKKKNLSRYEMITFKPGSYVRR 420
           VTKREYAME EWK+WSWRSEGD +MNGAVF+ SGDQTKGKKK LSRY+MI+FKPG+YVRR
Sbjct: 361 VTKREYAMEDEWKHWSWRSEGDELMNGAVFIPSGDQTKGKKKGLSRYDMISFKPGTYVRR 420

Query: 421 LTRFSGTLECVVGKPC 437
           L RFSGTLEC  GKPC
Sbjct: 421 LARFSGTLECTPGKPC 434

[Close]

BLAST of Tan0014447 vs. NCBI nr
Match: XP_004146792.2 (pectate lyase [Cucumis sativus] >KAE8647292.1 hypothetical protein Csa_003655 [Cucumis sativus])

HSP 1 Score: 792.3 bits (2045), Expect = 2.1e-225
Identity = 378/437 (86.50%), Postives = 401/437 (91.76%), Query Frame = 0


Query: 1   MAAFRTLLVVCSLLVFAVANTDVNEVDEYWKTRAREAQKAAQDAYIPNPETITDDFNAAV 60
           MA F +L  VCSLL F VAN +V+E DEYW+TRA EA+K AQ A+  NPE +TD  NAAV
Sbjct: 1   MAVFSSLF-VCSLLFFVVANANVDE-DEYWQTRATEAKKVAQGAFNANPEIVTDTLNAAV 60

Query: 61  GREMTGSNSTRRSL-KKYKGPCLATNPIDRCWRCDPNWANNRKKLAECPLGFGHQTIGGK 120
            R M G NSTRRSL KKY+GPCLATNPIDRCWRCD NWA NR+KLAECPLGFGHQTIGGK
Sbjct: 61  SRTMLGHNSTRRSLRKKYRGPCLATNPIDRCWRCDRNWAKNRRKLAECPLGFGHQTIGGK 120

Query: 121 RGPIYVVTDGSDDDIVNPKPGTLRHAVIQKGPLWIIFGRSMIIRLTQELMISSNKTIDAR 180
           RGPIYVVTD SDDD+VNPKPGTLRH VIQKGPLWIIFGRSM+IRL+QELMISS+KTIDAR
Sbjct: 121 RGPIYVVTDASDDDLVNPKPGTLRHGVIQKGPLWIIFGRSMVIRLSQELMISSHKTIDAR 180

Query: 181 GANVHIAFGAGLTIQFVNNVIIHGLHIHDIVSKSGGMIRDSVDHYGFRTQSDGDGISIFG 240
           GANVHIAFGAGLTIQFVNNVIIH LHIHDIVSKSGGMIRDSVDHYGFRTQSDGDGISIFG
Sbjct: 181 GANVHIAFGAGLTIQFVNNVIIHNLHIHDIVSKSGGMIRDSVDHYGFRTQSDGDGISIFG 240

Query: 241 SSHVWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNEVMLFGASDSYSEDSVMQVTL 300
           SSHVWIDHVSMSNCQDGLIDAIMGSTAITISN HFTHHNEVMLFGASDSY+ED +MQVTL
Sbjct: 241 SSHVWIDHVSMSNCQDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYAEDEIMQVTL 300

Query: 301 AFNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAK 360
           AFNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAK
Sbjct: 301 AFNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAK 360

Query: 361 NVTKREYAMESEWKNWSWRSEGDLMMNGAVFVASGDQTKGKKKNLSRYEMITFKPGSYVR 420
            +TKREYAME EWK+WSWRSEGD +MNGAVF+ASGDQTKGKKK LSRY+MI+FKPG+YVR
Sbjct: 361 QITKREYAMEDEWKHWSWRSEGDELMNGAVFIASGDQTKGKKKGLSRYDMISFKPGTYVR 420

Query: 421 RLTRFSGTLECVVGKPC 437
           RL R SGT+EC  GKPC
Sbjct: 421 RLVRLSGTIECTPGKPC 435

[Close]

BLAST of Tan0014447 vs. NCBI nr
Match: XP_038886315.1 (pectate lyase-like [Benincasa hispida])

HSP 1 Score: 783.5 bits (2022), Expect = 9.5e-223
Identity = 373/436 (85.55%), Postives = 399/436 (91.51%), Query Frame = 0


Query: 1   MAAFRTLLVVCSLLVFAVANTDVNEVDEYWKTRAREAQKAAQDAYIPNPETITDDFNAAV 60
           M  FRTL  VC LL F VAN DV+E DEYW+TRA EAQK AQDAY P+PE +TD+ NAAV
Sbjct: 1   MVGFRTLF-VCLLLFFVVANADVDE-DEYWRTRATEAQKVAQDAYNPDPEIVTDNLNAAV 60

Query: 61  GREMTGSNSTRRSLKKYKGPCLATNPIDRCWRCDPNWANNRKKLAECPLGFGHQTIGGKR 120
            R M G NSTRRSL  YKGPCLATNPIDRCWRC+ NWA NRKKLAECPLGFGH+TIGGKR
Sbjct: 61  SRVMIGRNSTRRSL-NYKGPCLATNPIDRCWRCNRNWARNRKKLAECPLGFGHKTIGGKR 120

Query: 121 GPIYVVTDGSDDDIVNPKPGTLRHAVIQKGPLWIIFGRSMIIRLTQELMISSNKTIDARG 180
           GPIYVVTD SDDD++NPKPGTLR+ VIQKGPLWIIFGRSMIIRL+QELMISS+KTIDARG
Sbjct: 121 GPIYVVTDASDDDLINPKPGTLRYGVIQKGPLWIIFGRSMIIRLSQELMISSDKTIDARG 180

Query: 181 ANVHIAFGAGLTIQFVNNVIIHGLHIHDIVSKSGGMIRDSVDHYGFRTQSDGDGISIFGS 240
           ANVHIA+GAGLTIQFVNNVIIH LHIHDIVSKSGGMIRDSVDHYGFRTQSDGDGISIFGS
Sbjct: 181 ANVHIAYGAGLTIQFVNNVIIHNLHIHDIVSKSGGMIRDSVDHYGFRTQSDGDGISIFGS 240

Query: 241 SHVWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNEVMLFGASDSYSEDSVMQVTLA 300
           SHVWIDH+SMSNCQDGLIDAIMGSTAITISN HFTHHNEVMLFGASDSY+ED++MQ+TLA
Sbjct: 241 SHVWIDHISMSNCQDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYAEDAIMQITLA 300

Query: 301 FNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKN 360
           FNHFGRGLVQRMPRCRWGFFHVVNN YTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAK 
Sbjct: 301 FNHFGRGLVQRMPRCRWGFFHVVNNHYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKL 360

Query: 361 VTKREYAMESEWKNWSWRSEGDLMMNGAVFVASGDQTKGKKKNLSRYEMITFKPGSYVRR 420
           VTKREYAMESEWK WSWRSEGD ++NGAVF+ SGDQTKGK+K LSRY+MI+FKPG+YVRR
Sbjct: 361 VTKREYAMESEWKKWSWRSEGDALVNGAVFIPSGDQTKGKRKGLSRYDMISFKPGTYVRR 420

Query: 421 LTRFSGTLECVVGKPC 437
           L RF+GTL C   KPC
Sbjct: 421 LVRFAGTLACAPRKPC 433

[Close]

BLAST of Tan0014447 vs. NCBI nr
Match: KAG7020735.1 (putative pectate lyase P59 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 762.3 bits (1967), Expect = 2.3e-216
Identity = 348/415 (83.86%), Postives = 388/415 (93.49%), Query Frame = 0


Query: 22  DVNEVDEYWKTRAREAQKAAQDAYIPNPETITDDFNAAVGREMTGSNSTRRSLKKYKGPC 81
           DV + DEYW+ RA EAQKAAQ A+ P+P+++ D+FNAAVG+E+  SN++RRSL++Y+GPC
Sbjct: 48  DVEKADEYWQKRAAEAQKAAQKAFNPDPDSVADNFNAAVGQEIVNSNNSRRSLRRYRGPC 107

Query: 82  LATNPIDRCWRCDPNWANNRKKLAECPLGFGHQTIGGKRGPIYVVTDGSDDDIVNPKPGT 141
           LATNPIDRCWRCDPNW  NRKKLAECPLGFGHQTIGGKRGPIYVVTD SD+++V+PKPGT
Sbjct: 108 LATNPIDRCWRCDPNWDKNRKKLAECPLGFGHQTIGGKRGPIYVVTDASDENLVDPKPGT 167

Query: 142 LRHAVIQKGPLWIIFGRSMIIRLTQELMISSNKTIDARGANVHIAFGAGLTIQFVNNVII 201
           LRHAV+Q GP WIIFGRSMIIRL+QEL+ISSNKTIDARG NVHIAFGAGLT+QFV+N+II
Sbjct: 168 LRHAVVQNGPRWIIFGRSMIIRLSQELIISSNKTIDARGFNVHIAFGAGLTLQFVHNIII 227

Query: 202 HGLHIHDIVSKSGGMIRDSVDHYGFRTQSDGDGISIFGSSHVWIDHVSMSNCQDGLIDAI 261
           HGLHIHDI+SKSGGMIRD+ DHYGFRTQSDGDGISIFG SH+WIDHVSM NCQDGLIDAI
Sbjct: 228 HGLHIHDIISKSGGMIRDAFDHYGFRTQSDGDGISIFGGSHIWIDHVSMYNCQDGLIDAI 287

Query: 262 MGSTAITISNSHFTHHNEVMLFGASDSYSEDSVMQVTLAFNHFGRGLVQRMPRCRWGFFH 321
           MGSTAITISNSHFTHHNEVMLFGASDSY EDS+MQ+TLAFNHFGRGLVQRMPRCR+GFFH
Sbjct: 288 MGSTAITISNSHFTHHNEVMLFGASDSYKEDSIMQITLAFNHFGRGLVQRMPRCRFGFFH 347

Query: 322 VVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKNVTKREYAMESEWKNWSWRSEG 381
           VVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNP+AK +TKREYA ESEWKNWSWRSEG
Sbjct: 348 VVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPSAKQITKREYADESEWKNWSWRSEG 407

Query: 382 DLMMNGAVFVASGDQTKGKKKNLSRYEMITFKPGSYVRRLTRFSGTLECVVGKPC 437
           DLM+NGA FV +GDQTKGKK + SRY+MI FKPG+YV+RLTRF+GTLEC VGKPC
Sbjct: 408 DLMINGAYFVPAGDQTKGKKSDFSRYDMIKFKPGAYVKRLTRFAGTLECQVGKPC 462

[Close]

BLAST of Tan0014447 vs. NCBI nr
Match: XP_022951907.1 (pectate lyase-like [Cucurbita moschata])

HSP 1 Score: 759.6 bits (1960), Expect = 1.5e-215
Identity = 346/415 (83.37%), Postives = 387/415 (93.25%), Query Frame = 0


Query: 22  DVNEVDEYWKTRAREAQKAAQDAYIPNPETITDDFNAAVGREMTGSNSTRRSLKKYKGPC 81
           D+ + DEYW+ RA EAQKAAQ A+ P+P+++ D+FNAAVG+E+  SN++RRSL++Y+GPC
Sbjct: 42  DLEKADEYWQKRAAEAQKAAQKAFNPDPDSVADNFNAAVGQEIVNSNNSRRSLRRYRGPC 101

Query: 82  LATNPIDRCWRCDPNWANNRKKLAECPLGFGHQTIGGKRGPIYVVTDGSDDDIVNPKPGT 141
           LATNPIDRCWRCDPNW  NRKKLAECPLGFGHQTIGGKRGPIYVVTD SD+++V+PKPGT
Sbjct: 102 LATNPIDRCWRCDPNWDKNRKKLAECPLGFGHQTIGGKRGPIYVVTDASDENLVDPKPGT 161

Query: 142 LRHAVIQKGPLWIIFGRSMIIRLTQELMISSNKTIDARGANVHIAFGAGLTIQFVNNVII 201
           LRHAV+Q GP WIIFGRSMIIRL+QEL+ISSNKTID RG NVHIAFGAGLT+QFV+N+II
Sbjct: 162 LRHAVVQNGPRWIIFGRSMIIRLSQELIISSNKTIDGRGFNVHIAFGAGLTLQFVHNIII 221

Query: 202 HGLHIHDIVSKSGGMIRDSVDHYGFRTQSDGDGISIFGSSHVWIDHVSMSNCQDGLIDAI 261
           HGLHIHDI+SKSGGMIRD+ DHYGFRTQSDGDGISIFG SH+WIDHVSM NCQDGLIDAI
Sbjct: 222 HGLHIHDIISKSGGMIRDAFDHYGFRTQSDGDGISIFGGSHIWIDHVSMYNCQDGLIDAI 281

Query: 262 MGSTAITISNSHFTHHNEVMLFGASDSYSEDSVMQVTLAFNHFGRGLVQRMPRCRWGFFH 321
           MGSTAITISNSHFTHHNEVMLFGASDSY EDS+MQ+TLAFNHFGRGLVQRMPRCR+GFFH
Sbjct: 282 MGSTAITISNSHFTHHNEVMLFGASDSYKEDSIMQITLAFNHFGRGLVQRMPRCRFGFFH 341

Query: 322 VVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKNVTKREYAMESEWKNWSWRSEG 381
           VVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNP+AK +TKREYA ESEWKNWSWRSEG
Sbjct: 342 VVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPSAKQITKREYADESEWKNWSWRSEG 401

Query: 382 DLMMNGAVFVASGDQTKGKKKNLSRYEMITFKPGSYVRRLTRFSGTLECVVGKPC 437
           DLM+NGA FV +GDQTKGKK + SRY+MI FKPG+YV+RLTRF+GTLEC VGKPC
Sbjct: 402 DLMINGAYFVPAGDQTKGKKSDFSRYDMIKFKPGAYVKRLTRFAGTLECQVGKPC 456

[Close]

BLAST of Tan0014447 vs. ExPASy TrEMBL
Match: A0A1S3C3Z5 (Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103496402 PE=3 SV=1)

HSP 1 Score: 800.4 bits (2066), Expect = 3.7e-228
Identity = 380/436 (87.16%), Postives = 402/436 (92.20%), Query Frame = 0


Query: 1   MAAFRTLLVVCSLLVFAVANTDVNEVDEYWKTRAREAQKAAQDAYIPNPETITDDFNAAV 60
           MA F TL  VCSLL F VAN +V+E DEYW TRA EA+K A+ A+ PNPE +TD+ NAAV
Sbjct: 1   MAGFSTLF-VCSLLFFVVANANVDE-DEYWNTRATEAKKVAKSAFNPNPEIVTDNLNAAV 60

Query: 61  GREMTGSNSTRRSLKKYKGPCLATNPIDRCWRCDPNWANNRKKLAECPLGFGHQTIGGKR 120
            R M G NSTRRSLKKY+GPCLATNPIDRCWRCD NWA NRKKLAECPLGFGHQTIGGKR
Sbjct: 61  SRAMVGRNSTRRSLKKYRGPCLATNPIDRCWRCDRNWAKNRKKLAECPLGFGHQTIGGKR 120

Query: 121 GPIYVVTDGSDDDIVNPKPGTLRHAVIQKGPLWIIFGRSMIIRLTQELMISSNKTIDARG 180
           GPIYVVTD SDDD+VNPKPGTLR+ VIQKGPLWIIFGRSMIIRL+QELMISS+KTIDARG
Sbjct: 121 GPIYVVTDASDDDLVNPKPGTLRYGVIQKGPLWIIFGRSMIIRLSQELMISSDKTIDARG 180

Query: 181 ANVHIAFGAGLTIQFVNNVIIHGLHIHDIVSKSGGMIRDSVDHYGFRTQSDGDGISIFGS 240
           ANVHIAFGAGLTIQFVNNVIIH LHIHDI+SKSGGMIRDSVDHYGFRTQSDGDGISIFGS
Sbjct: 181 ANVHIAFGAGLTIQFVNNVIIHNLHIHDIISKSGGMIRDSVDHYGFRTQSDGDGISIFGS 240

Query: 241 SHVWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNEVMLFGASDSYSEDSVMQVTLA 300
           SHVWIDHVSMSNC+DGLIDAIMGSTAITISN HFTHHNEVMLFGASDSY+ED +MQVTLA
Sbjct: 241 SHVWIDHVSMSNCEDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYAEDEIMQVTLA 300

Query: 301 FNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKN 360
           FNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAK 
Sbjct: 301 FNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKQ 360

Query: 361 VTKREYAMESEWKNWSWRSEGDLMMNGAVFVASGDQTKGKKKNLSRYEMITFKPGSYVRR 420
           VTKREYAME EWK+WSWRSEGD +MNGAVF+ SGDQTKGKKK LSRY+MI+FKPG+YVRR
Sbjct: 361 VTKREYAMEDEWKHWSWRSEGDELMNGAVFIPSGDQTKGKKKGLSRYDMISFKPGTYVRR 420

Query: 421 LTRFSGTLECVVGKPC 437
           L RFSGTLEC  GKPC
Sbjct: 421 LARFSGTLECTPGKPC 434

[Close]

BLAST of Tan0014447 vs. ExPASy TrEMBL
Match: A0A6J1GIY2 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111454644 PE=3 SV=1)

HSP 1 Score: 759.6 bits (1960), Expect = 7.1e-216
Identity = 346/415 (83.37%), Postives = 387/415 (93.25%), Query Frame = 0


Query: 22  DVNEVDEYWKTRAREAQKAAQDAYIPNPETITDDFNAAVGREMTGSNSTRRSLKKYKGPC 81
           D+ + DEYW+ RA EAQKAAQ A+ P+P+++ D+FNAAVG+E+  SN++RRSL++Y+GPC
Sbjct: 42  DLEKADEYWQKRAAEAQKAAQKAFNPDPDSVADNFNAAVGQEIVNSNNSRRSLRRYRGPC 101

Query: 82  LATNPIDRCWRCDPNWANNRKKLAECPLGFGHQTIGGKRGPIYVVTDGSDDDIVNPKPGT 141
           LATNPIDRCWRCDPNW  NRKKLAECPLGFGHQTIGGKRGPIYVVTD SD+++V+PKPGT
Sbjct: 102 LATNPIDRCWRCDPNWDKNRKKLAECPLGFGHQTIGGKRGPIYVVTDASDENLVDPKPGT 161

Query: 142 LRHAVIQKGPLWIIFGRSMIIRLTQELMISSNKTIDARGANVHIAFGAGLTIQFVNNVII 201
           LRHAV+Q GP WIIFGRSMIIRL+QEL+ISSNKTID RG NVHIAFGAGLT+QFV+N+II
Sbjct: 162 LRHAVVQNGPRWIIFGRSMIIRLSQELIISSNKTIDGRGFNVHIAFGAGLTLQFVHNIII 221

Query: 202 HGLHIHDIVSKSGGMIRDSVDHYGFRTQSDGDGISIFGSSHVWIDHVSMSNCQDGLIDAI 261
           HGLHIHDI+SKSGGMIRD+ DHYGFRTQSDGDGISIFG SH+WIDHVSM NCQDGLIDAI
Sbjct: 222 HGLHIHDIISKSGGMIRDAFDHYGFRTQSDGDGISIFGGSHIWIDHVSMYNCQDGLIDAI 281

Query: 262 MGSTAITISNSHFTHHNEVMLFGASDSYSEDSVMQVTLAFNHFGRGLVQRMPRCRWGFFH 321
           MGSTAITISNSHFTHHNEVMLFGASDSY EDS+MQ+TLAFNHFGRGLVQRMPRCR+GFFH
Sbjct: 282 MGSTAITISNSHFTHHNEVMLFGASDSYKEDSIMQITLAFNHFGRGLVQRMPRCRFGFFH 341

Query: 322 VVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKNVTKREYAMESEWKNWSWRSEG 381
           VVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNP+AK +TKREYA ESEWKNWSWRSEG
Sbjct: 342 VVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPSAKQITKREYADESEWKNWSWRSEG 401

Query: 382 DLMMNGAVFVASGDQTKGKKKNLSRYEMITFKPGSYVRRLTRFSGTLECVVGKPC 437
           DLM+NGA FV +GDQTKGKK + SRY+MI FKPG+YV+RLTRF+GTLEC VGKPC
Sbjct: 402 DLMINGAYFVPAGDQTKGKKSDFSRYDMIKFKPGAYVKRLTRFAGTLECQVGKPC 456

[Close]

BLAST of Tan0014447 vs. ExPASy TrEMBL
Match: A0A6J1G495 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111450600 PE=3 SV=1)

HSP 1 Score: 758.4 bits (1957), Expect = 1.6e-215
Identity = 362/440 (82.27%), Postives = 390/440 (88.64%), Query Frame = 0


Query: 1   MAAFRTLLVVCSLLVFAV----ANTDVNEVDEYWKTRAREAQKAAQDAYIPNPETITDDF 60
           MA FR +L V SLL F      AN DV++ DEYW TRA EA+K AQDAY P PE++T+  
Sbjct: 1   MAGFR-ILFVFSLLYFVAAYTDANVDVDDHDEYWLTRANEAKKVAQDAYNPKPESVTEHL 60

Query: 61  NAAVGREMTGSNSTRRSLKKYKGPCLATNPIDRCWRCDPNWANNRKKLAECPLGFGHQTI 120
           N AV   M G NSTRRSL KYKGPCL TNPIDRCWRCD NWANNRKKLAECPLGFGHQTI
Sbjct: 61  NVAVRLAMVGDNSTRRSLNKYKGPCLKTNPIDRCWRCDKNWANNRKKLAECPLGFGHQTI 120

Query: 121 GGKRGPIYVVTDGSDDDIVNPKPGTLRHAVIQKGPLWIIFGRSMIIRLTQELMISSNKTI 180
           GGKRG IYVVTD SDD++V+PKPGTLRH VIQKGPLWIIFGRSM+IRL QELMISS+KTI
Sbjct: 121 GGKRGRIYVVTDASDDNLVDPKPGTLRHGVIQKGPLWIIFGRSMVIRLAQELMISSDKTI 180

Query: 181 DARGANVHIAFGAGLTIQFVNNVIIHGLHIHDIVSKSGGMIRDSVDHYGFRTQSDGDGIS 240
           DARG NVHIA+GAGLTIQF +NVIIHGLHIH+I+SKSGG IRDSVDHYGFRTQSDGDGIS
Sbjct: 181 DARGFNVHIAYGAGLTIQFAHNVIIHGLHIHNIISKSGGYIRDSVDHYGFRTQSDGDGIS 240

Query: 241 IFGSSHVWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNEVMLFGASDSYSEDSVMQ 300
           IFGSSH+WIDHVSM  CQDGLIDAIMGST ITISNSHFTHHNE MLFGASDSY ED +MQ
Sbjct: 241 IFGSSHIWIDHVSMYACQDGLIDAIMGSTGITISNSHFTHHNEAMLFGASDSYKEDEIMQ 300

Query: 301 VTLAFNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNP 360
           +TLAFNHFGRGLVQRMPRCR+GFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAP N 
Sbjct: 301 ITLAFNHFGRGLVQRMPRCRFGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPQNI 360

Query: 361 NAKNVTKREYAMESEWKNWSWRSEGDLMMNGAVFVASGDQTKGKKKNLSRYEMITFKPGS 420
           NAK +TKREYA ESEWK WSWRSEGDL++NGAVFV SGDQTKGKKK+LSRY+MI+FKPGS
Sbjct: 361 NAKTITKREYADESEWKKWSWRSEGDLLLNGAVFVGSGDQTKGKKKDLSRYDMISFKPGS 420

Query: 421 YVRRLTRFSGTLECVVGKPC 437
           YV+RL RF+GTLEC VGKPC
Sbjct: 421 YVKRLVRFAGTLECRVGKPC 439

[Close]

BLAST of Tan0014447 vs. ExPASy TrEMBL
Match: A0A6J1KG97 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111493968 PE=3 SV=1)

HSP 1 Score: 751.9 bits (1940), Expect = 1.5e-213
Identity = 356/436 (81.65%), Postives = 386/436 (88.53%), Query Frame = 0


Query: 1   MAAFRTLLVVCSLLVFAVANTDVNEVDEYWKTRAREAQKAAQDAYIPNPETITDDFNAAV 60
           MA FR L V       A A+ DV++ DEYW TRA EA++ A+DAY PNPE++T+  N AV
Sbjct: 1   MAGFRILFVFSLYFFAAYASVDVDDHDEYWLTRANEAKEVAKDAYNPNPESVTEHLNVAV 60

Query: 61  GREMTGSNSTRRSLKKYKGPCLATNPIDRCWRCDPNWANNRKKLAECPLGFGHQTIGGKR 120
              M G NSTRRSL KYKGPCL TNPIDRCWRCD NWANNRKKLAECPLGFGHQTIGGKR
Sbjct: 61  RLAMVGDNSTRRSLNKYKGPCLKTNPIDRCWRCDKNWANNRKKLAECPLGFGHQTIGGKR 120

Query: 121 GPIYVVTDGSDDDIVNPKPGTLRHAVIQKGPLWIIFGRSMIIRLTQELMISSNKTIDARG 180
           G IYVVTD SDD++V+PK GTLR+ VIQKGPLWIIFGRSM+IRL QELMISS+KTIDARG
Sbjct: 121 GRIYVVTDASDDNLVDPKLGTLRYGVIQKGPLWIIFGRSMVIRLAQELMISSDKTIDARG 180

Query: 181 ANVHIAFGAGLTIQFVNNVIIHGLHIHDIVSKSGGMIRDSVDHYGFRTQSDGDGISIFGS 240
            NVHIA+GAGLTIQF +NVIIHGLHIH+I+SKSGG IRDSVDHYGFRTQSDGDGISIFGS
Sbjct: 181 FNVHIAYGAGLTIQFAHNVIIHGLHIHNIISKSGGYIRDSVDHYGFRTQSDGDGISIFGS 240

Query: 241 SHVWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNEVMLFGASDSYSEDSVMQVTLA 300
           SHVWIDHVSM  CQDGLIDAIMGST ITISNSHFTHHNE MLFGASDSY ED +MQ+TLA
Sbjct: 241 SHVWIDHVSMYACQDGLIDAIMGSTGITISNSHFTHHNEAMLFGASDSYKEDEIMQITLA 300

Query: 301 FNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKN 360
           FNHFGRGLVQRMPRCR+GFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAP N NAK 
Sbjct: 301 FNHFGRGLVQRMPRCRFGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPQNINAKT 360

Query: 361 VTKREYAMESEWKNWSWRSEGDLMMNGAVFVASGDQTKGKKKNLSRYEMITFKPGSYVRR 420
           +TKREYA ESEWK WSWRSEGDL+MNGAVFV SGDQTKGKKK+LSRY+MI++KPGSYV+R
Sbjct: 361 ITKREYADESEWKKWSWRSEGDLLMNGAVFVGSGDQTKGKKKDLSRYDMISYKPGSYVKR 420

Query: 421 LTRFSGTLECVVGKPC 437
           L RF+GTLEC VGKPC
Sbjct: 421 LVRFAGTLECRVGKPC 436

[Close]

BLAST of Tan0014447 vs. ExPASy TrEMBL
Match: A0A5A7UQS9 (Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold360G00930 PE=3 SV=1)

HSP 1 Score: 733.0 bits (1891), Expect = 7.2e-208
Identity = 341/373 (91.42%), Postives = 356/373 (95.44%), Query Frame = 0


Query: 64  MTGSNSTRRSLKKYKGPCLATNPIDRCWRCDPNWANNRKKLAECPLGFGHQTIGGKRGPI 123
           M G NSTRRSLKKY+GPCLATNPIDRCWRCD NWA NRKKLAECPLGFGHQTIGGKRGPI
Sbjct: 1   MVGRNSTRRSLKKYRGPCLATNPIDRCWRCDRNWAKNRKKLAECPLGFGHQTIGGKRGPI 60

Query: 124 YVVTDGSDDDIVNPKPGTLRHAVIQKGPLWIIFGRSMIIRLTQELMISSNKTIDARGANV 183
           YVVTD SDDD+VNPKPGTLR+ VIQKGPLWIIFGRSMIIRL+QELMISS+KTIDARGANV
Sbjct: 61  YVVTDASDDDLVNPKPGTLRYGVIQKGPLWIIFGRSMIIRLSQELMISSDKTIDARGANV 120

Query: 184 HIAFGAGLTIQFVNNVIIHGLHIHDIVSKSGGMIRDSVDHYGFRTQSDGDGISIFGSSHV 243
           HIAFGAGLTIQFVNNVIIH LHIHDI+SKSGGMIRDSVDHYGFRTQSDGDGISIFGSSHV
Sbjct: 121 HIAFGAGLTIQFVNNVIIHNLHIHDIISKSGGMIRDSVDHYGFRTQSDGDGISIFGSSHV 180

Query: 244 WIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNEVMLFGASDSYSEDSVMQVTLAFNH 303
           WIDHVSMSNC+DGLIDAIMGSTAITISN HFTHHNEVMLFGASDSY+ED +MQVTLAFNH
Sbjct: 181 WIDHVSMSNCEDGLIDAIMGSTAITISNCHFTHHNEVMLFGASDSYAEDEIMQVTLAFNH 240

Query: 304 FGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKNVTK 363
           FGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAK VTK
Sbjct: 241 FGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKQVTK 300

Query: 364 REYAMESEWKNWSWRSEGDLMMNGAVFVASGDQTKGKKKNLSRYEMITFKPGSYVRRLTR 423
           REYAME EWK+WSWRSEGD +MNGAVF+ SGDQTKGKKK LSRY+MI+FKPG+YVRRL R
Sbjct: 301 REYAMEDEWKHWSWRSEGDELMNGAVFIPSGDQTKGKKKGLSRYDMISFKPGTYVRRLAR 360

Query: 424 FSGTLECVVGKPC 437
           FSGTLEC  GKPC
Sbjct: 361 FSGTLECTPGKPC 373

[Close]

BLAST of Tan0014447 vs. TAIR 10
Match: AT3G01270.1 (Pectate lyase family protein )

HSP 1 Score: 551.2 bits (1419), Expect = 7.4e-157
Identity = 274/471 (58.17%), Postives = 340/471 (72.19%), Query Frame = 0


Query: 4   FRTLLVVC-SLLVFAVANTDVNEVDEYWKTRAREAQKAAQDAYIPNPETITDDFN----- 63
           F+ + V+C + L+  +     +E DEYW  +A EA+K    AY P+P  I D F+     
Sbjct: 7   FKLVCVICIASLIPTIRANVADETDEYWVNKANEARKHTLMAYHPDPYEIVDHFHERHYD 66

Query: 64  -----------AAVGRE--------MTGSNSTRRSL------------KKYKGPCLATNP 123
                       AV  E         + +NSTRRSL             K  GPC A+NP
Sbjct: 67  NSTDVEGTEEEKAVASEEEDVIEMISSPTNSTRRSLTGRGKGKGKGKWSKLTGPCTASNP 126

Query: 124 IDRCWRCDPNWANNRKKLAECPLGFGHQTIGGKRGPIYVVTDGSDDDIVNPKPGTLRHAV 183
           ID+CWRC P+WA  RKKL  C  GFG++T GGKRG IYVVT   DDD+VNP+PGTLRHAV
Sbjct: 127 IDKCWRCQPDWARRRKKLVHCVRGFGYRTTGGKRGRIYVVTSPRDDDMVNPRPGTLRHAV 186

Query: 184 IQKGPLWIIFGRSMIIRLTQELMISSNKTIDARGANVHIAFGAGLTIQFVNNVIIHGLHI 243
           IQK PLWI+F   M IRL+QELMI+S+KTIDARGANVHIA+GAG+T+Q+V+N+IIHGLH+
Sbjct: 187 IQKEPLWIVFKHDMSIRLSQELMITSDKTIDARGANVHIAYGAGITMQYVHNIIIHGLHV 246

Query: 244 HDIVSKSGGMIRDSVDHYGFRTQSDGDGISIFGSSHVWIDHVSMSNCQDGLIDAIMGSTA 303
           H IV  SGG+IRDS++H+G R ++DGDGISIFG++++W+DH+SMS CQDGLIDAIMGSTA
Sbjct: 247 HHIVKSSGGLIRDSINHFGHRGEADGDGISIFGATNIWLDHISMSKCQDGLIDAIMGSTA 306

Query: 304 ITISNSHFTHHNEVMLFGASDSYSEDSVMQVTLAFNHFGRGLVQRMPRCRWGFFHVVNND 363
           ITISNSHFTHHN+VML GA ++  +D  MQVT+A+NHFG+GLVQRMPR RWGF HVVNND
Sbjct: 307 ITISNSHFTHHNDVMLLGAQNNNMDDKKMQVTVAYNHFGKGLVQRMPRVRWGFVHVVNND 366

Query: 364 YTHWLMYAIGGSQHPTIVSQGNRFIAPPN-PNAKNVTKREYAMESEWKNWSWRSEGDLMM 423
           YTHW +YAIGGSQ PTI+S GNRFIAPP+  + + VTKR+YA ESEWKNW+WRSE D+ M
Sbjct: 367 YTHWELYAIGGSQGPTILSHGNRFIAPPHKQHYREVTKRDYASESEWKNWNWRSEKDVFM 426

Query: 424 NGAVFVASGDQTKGKKKNLSRYEMITFKPGSYVRRLTRFSGTLECVVGKPC 437
           N A F  SG+     K + SR +MI  K G  V +LT+++G L+C VGK C
Sbjct: 427 NNAYFRQSGN--PHFKCSHSRQQMIKPKNGMAVSKLTKYAGALDCRVGKAC 475

[Close]

BLAST of Tan0014447 vs. TAIR 10
Match: AT5G15110.1 (Pectate lyase family protein )

HSP 1 Score: 546.6 bits (1407), Expect = 1.8e-155
Identity = 271/467 (58.03%), Postives = 332/467 (71.09%), Query Frame = 0


Query: 5   RTLLVVCSLLVFAVANTDVNEVDEYWKTRAREAQKAAQDAYIPNPETITDDFN------- 64
           + +   C  ++      +++E+DEYW  RA EA++    AY  +P  I D F+       
Sbjct: 8   KLMFTFCIAVLIPTIRGNISELDEYWSQRADEAREFTLQAYHSDPYEIVDHFHERHYDNS 67

Query: 65  -----------------AAVGREMTGS--NSTRRSLK--------KYKGPCLATNPIDRC 124
                                 EM GS  NSTRRSL+        K KGPC A+NPID+C
Sbjct: 68  TDVTTPEEDGDAKPEEEEKEFIEMLGSSTNSTRRSLRGKGKGKWSKLKGPCTASNPIDKC 127

Query: 125 WRCDPNWANNRKKLAECPLGFGHQTIGGKRGPIYVVTDGSDDDIVNPKPGTLRHAVIQKG 184
           WRC  +WA  RKKL  C  GFGH+T GGKRG IYVVT   D+D+VNPKPGTLRHAVIQK 
Sbjct: 128 WRCRSDWAKRRKKLTRCVRGFGHRTTGGKRGRIYVVTSNLDEDMVNPKPGTLRHAVIQKE 187

Query: 185 PLWIIFGRSMIIRLTQELMISSNKTIDARGANVHIAFGAGLTIQFVNNVIIHGLHIHDIV 244
           PLWIIF   M IRL QEL+I+S+KTIDARGANVH+A GAG+T+QFV NV+IHGLHIH I 
Sbjct: 188 PLWIIFKNDMSIRLNQELLINSHKTIDARGANVHVAHGAGITMQFVKNVVIHGLHIHHIS 247

Query: 245 SKSGGMIRDSVDHYGFRTQSDGDGISIFGSSHVWIDHVSMSNCQDGLIDAIMGSTAITIS 304
             SGGMIRDSVDH+G RT++DGDG+SI+GSS++W+DH+SMS CQDGLIDAI+GST ITIS
Sbjct: 248 ESSGGMIRDSVDHFGMRTRADGDGLSIYGSSNIWLDHISMSKCQDGLIDAIVGSTGITIS 307

Query: 305 NSHFTHHNEVMLFGASDSYSEDSVMQVTLAFNHFGRGLVQRMPRCRWGFFHVVNNDYTHW 364
           NSHFTHHN+VML GA ++   D  MQVT+A+NHFG+GLVQRMPR RWGF HVVNNDYTHW
Sbjct: 308 NSHFTHHNDVMLLGAQNTNEADKHMQVTVAYNHFGKGLVQRMPRIRWGFVHVVNNDYTHW 367

Query: 365 LMYAIGGSQHPTIVSQGNRFIAPPN-PNAKNVTKREYAMESEWKNWSWRSEGDLMMNGAV 424
            +YAIGGSQ PTI+S GNRFIAPP+ P+ + VTKR+YA E EWK+W+WRS+ D+ MNGA 
Sbjct: 368 ELYAIGGSQGPTILSHGNRFIAPPHKPHYREVTKRDYASEDEWKHWNWRSDKDVFMNGAY 427

Query: 425 FVASGDQTKGKKKNLSRYEMITFKPGSYVRRLTRFSGTLECVVGKPC 437
           F  SG+     K   +R +MI  K G  V +LT+++G L+C VG+ C
Sbjct: 428 FRQSGNPQ--YKCAHTRQQMIKPKNGLAVSKLTKYAGALDCRVGRRC 472

[Close]

BLAST of Tan0014447 vs. TAIR 10
Match: AT1G14420.1 (Pectate lyase family protein )

HSP 1 Score: 528.5 bits (1360), Expect = 5.2e-150
Identity = 258/425 (60.71%), Postives = 308/425 (72.47%), Query Frame = 0


Query: 27  DEYWKTRAREAQKAAQDAYIPNPETITDDFNAAVGREMTGS---NSTRRSL--------- 86
           D YW  R  +A K    +Y P+P  +T+ FN  V   +  S   N TRR L         
Sbjct: 35  DSYWTQRQSDALKQTIGSYDPHPLNVTNHFNYHVNIAVDASESRNDTRRELTQVRSGRKT 94

Query: 87  KKYKGPCLATNPIDRCWRCDPNWANNRKKLAECPLGFGHQTIGGKRGPIYVVTDGSDDDI 146
            K  G CLA NPID CWRCD NWANNRKKLA+C LGFG +T GGK GPIYVV D SD+D+
Sbjct: 95  HKSSGKCLAYNPIDNCWRCDRNWANNRKKLADCVLGFGRRTTGGKDGPIYVVKDASDNDL 154

Query: 147 VNPKPGTLRHAVIQKGPLWIIFGRSMIIRLTQELMISSNKTIDARGANVHIAFGAGLTIQ 206
           +NPKPGTLRHAV + GPLWIIF RSMII+L QELMI+S+KTID RGA V+I  GAGLT+Q
Sbjct: 155 INPKPGTLRHAVTRDGPLWIIFARSMIIKLQQELMITSDKTIDGRGARVYIMEGAGLTLQ 214

Query: 207 FVNNVIIHGLHIHDIVSKSGGMIRDSVDHYGFRTQSDGDGISIFGSSHVWIDHVSMSNCQ 266
           FVNNVIIH +++  IV  +GG+IRDS  H G RT+SDGDGIS+FG++++WIDHVSM+ C 
Sbjct: 215 FVNNVIIHNIYVKHIVPGNGGLIRDSEAHIGLRTKSDGDGISLFGATNIWIDHVSMTRCA 274

Query: 267 DGLIDAIMGSTAITISNSHFTHHNEVMLFGASDSYSEDSVMQVTLAFNHFGRGLVQRMPR 326
           DG+IDAI GSTA+TISNSHFT H EVMLFGA D +  D  MQ+T+AFNHFG+ L QRMPR
Sbjct: 275 DGMIDAIDGSTAVTISNSHFTDHQEVMLFGARDEHVIDKKMQITVAFNHFGKRLEQRMPR 334

Query: 327 CRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQGNRFIAPPNPNAKNVTKREYAMESEWKN 386
           CR+G  HVVNNDYTHW MYAIGG+ +PTI+SQGNRFIAPPN  AK +TKREY    EWK+
Sbjct: 335 CRYGTIHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIAPPNEEAKQITKREYTPYGEWKS 394

Query: 387 WSWRSEGDLMMNGAVFVASGDQ---TKGKKKNLSRYEMITFKPGSYVRRLTRFSGTLECV 437
           W+W+SEGD  +NGA FV SG     +   K  L     I  KPG+ VR+LT  +G L C 
Sbjct: 395 WNWQSEGDYFLNGAYFVQSGKANAWSSKPKTPLPNKFTIRPKPGTMVRKLTMDAGVLGCK 454

[Close]

BLAST of Tan0014447 vs. TAIR 10
Match: AT2G02720.1 (Pectate lyase family protein )

HSP 1 Score: 502.7 bits (1293), Expect = 3.0e-142
Identity = 255/452 (56.42%), Postives = 318/452 (70.35%), Query Frame = 0


Query: 1   MAAFRTLLVVCSL-LVFAVANTDVNEVDEYWKTRAREAQKAAQDAYIPNPETITDDFN-- 60
           + ++  + V  SL +V       + E DEYW  R   A +   ++Y PNPE +TD FN  
Sbjct: 4   LGSYVFVFVALSLTVVVPSVQAHIAEYDEYWTQRQTNALRETLESYDPNPENVTDHFNYH 63

Query: 61  AAVGREMTG-SNSTRRSLK---------KYKGPCLATNPIDRCWRCDPNWANNRKKLAEC 120
           AA+  E TG  N TRR L+         +  G   + N ID+CWR D NW  NRKKLA+C
Sbjct: 64  AALAMETTGIVNETRRDLRQVGRGKKTTRRGGRFESLNAIDKCWRGDKNWDKNRKKLADC 123

Query: 121 PLGFGHQTIGGKRGPIYVVTDGSDDDIVNPKPGTLRHAVIQKGPLWIIFGRSMIIRLTQE 180
            LGFG +T GGK GPIYVVTD SD+D++ PKPGT+RHAV +  PLWIIF RSMII+L QE
Sbjct: 124 VLGFGRKTTGGKNGPIYVVTDPSDNDLLKPKPGTIRHAVTRDRPLWIIFARSMIIKLQQE 183

Query: 181 LMISSNKTIDARGANVHIAFGAGLTIQFVNNVIIHGLHIHDIVSKSGGMIRDSVDHYGFR 240
           L+I+++KTID RGA ++I  GAGLT+QFV NVIIH +HI  I   +GG+I DS  H+G R
Sbjct: 184 LIITNDKTIDGRGAKIYITGGAGLTLQFVRNVIIHNIHIKQIKRGAGGLIIDSEQHFGLR 243

Query: 241 TQSDGDGISIFGSSHVWIDHVSMSNCQDGLIDAIMGSTAITISNSHFTHHNEVMLFGASD 300
           T SDGDGI+IFG+++VWIDHVSM++C DG+IDAIMGSTAITISNSHFT H+EVMLFG ++
Sbjct: 244 TVSDGDGINIFGATNVWIDHVSMTDCSDGMIDAIMGSTAITISNSHFTDHDEVMLFGGTN 303

Query: 301 SYSEDSVMQVTLAFNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIVSQG 360
               D  MQ+T+AFNHFG+ L QRMPR R+G  HVVNNDYTHW MYAIGG+ +PTI+SQG
Sbjct: 304 KDVIDKKMQITVAFNHFGKRLKQRMPRVRFGLVHVVNNDYTHWEMYAIGGNMNPTIISQG 363

Query: 361 NRFIAPPNPNAKNVTKREYAMESEWKNWSWRSEGDLMMNGAVFVASGDQ---TKGKKKNL 420
           NRFIAPP  ++K VTKREY    EWK+W+W+SE D  +NGA FV SG     +   K  +
Sbjct: 364 NRFIAPPIEDSKQVTKREYTPYPEWKSWNWQSEKDYFLNGAYFVQSGKANAWSATPKNPI 423

Query: 421 SRYEMITFKPGSYVRRLTRFSGTLECVVGKPC 437
            R   I  +PG+ VRRLT+ +GTL C  GK C
Sbjct: 424 PRKFAIRPQPGTKVRRLTKDAGTLGCKPGKSC 455

[Close]

BLAST of Tan0014447 vs. TAIR 10
Match: AT4G13710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 494.6 bits (1272), Expect = 8.2e-140
Identity = 239/406 (58.87%), Postives = 292/406 (71.92%), Query Frame = 0


Query: 32  TRAREAQKAAQDAYIPNPETITDDFNAAVGREMTGSNST-RRSLKKYKGPCLATNPIDRC 91
           TR     +  Q A   +P+ + D+    V  +M+  N T RR L  +   C   NPID C
Sbjct: 72  TRLDGEPQQQQHAVADDPDMVADEVAKLV--QMSEQNRTARRKLGFFS--CGTGNPIDDC 131

Query: 92  WRCDPNWANNRKKLAECPLGFGHQTIGGKRGPIYVVTDGSDDDIVNPKPGTLRHAVIQKG 151
           WRCD NW  NRK+LA+C +GFG   IGG+ G  Y+VTD +D+D+VNPKPGTLRHAVIQ+ 
Sbjct: 132 WRCDRNWHKNRKRLADCGIGFGRNAIGGRDGRFYIVTDPTDEDVVNPKPGTLRHAVIQEE 191

Query: 152 PLWIIFGRSMIIRLTQELMISSNKTIDARGANVHIAFGAGLTIQFVNNVIIHGLHIHDIV 211
           PLWI+F R M+I L QEL+++S KTIDARG+NVHIA GA +TIQF+ NVIIHGLHIHD  
Sbjct: 192 PLWIVFKRDMVIELKQELIMNSFKTIDARGSNVHIANGACITIQFITNVIIHGLHIHDCK 251

Query: 212 SKSGGMIRDSVDHYGFRTQSDGDGISIFGSSHVWIDHVSMSNCQDGLIDAIMGSTAITIS 271
                M+R S  H+G+RT +DGD +SIFGSSH+WIDH S+S+C DGL+DA+MGSTAIT+S
Sbjct: 252 PTGNAMVRSSPSHFGWRTMADGDAVSIFGSSHIWIDHNSLSHCADGLVDAVMGSTAITVS 311

Query: 272 NSHFTHHNEVMLFGASDSYSEDSVMQVTLAFNHFGRGLVQRMPRCRWGFFHVVNNDYTHW 331
           N+HFTHHNEVML G SDSY++D +MQVT+A+NHFG GLVQRMPRCR G+FHVVNNDYTHW
Sbjct: 312 NNHFTHHNEVMLLGHSDSYTKDKLMQVTIAYNHFGEGLVQRMPRCRHGYFHVVNNDYTHW 371

Query: 332 LMYAIGGSQHPTIVSQGNRFIAPPNPNAKNVTKREYAMESEWKNWSWRSEGDLMMNGAVF 391
            MYAIGGS  PTI SQGNR+ AP +  AK VTKR     SEWK W+WRSEGDL++NGA F
Sbjct: 372 EMYAIGGSAEPTINSQGNRYAAPMDRFAKEVTKRVETDASEWKKWNWRSEGDLLLNGAFF 431

Query: 392 VASGDQTKGKKKNLSRYEMITFKPGSYVRRLTRFSGTLECVVGKPC 437
             SG    G   +  R   +  KP S V  +T  +G L C  G+PC
Sbjct: 432 RPSG---AGASASYGRASSLAAKPSSMVDTITSTAGALGCRKGRPC 470

The following BLAST results are available for this feature:

BLAST of Tan0014447 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P15722	2.2e-161	64.94	Probable pectate lyase P59 OS=Solanum lycopersicum OX=4081 GN=LAT59 PE=2 SV=1	[more]
P40973	1.9e-157	62.47	Pectate lyase OS=Lilium longiflorum OX=4690 PE=2 SV=1	[more]
Q9SRH4	1.0e-155	58.17	Probable pectate lyase 7 OS=Arabidopsis thaliana OX=3702 GN=At3g01270 PE=2 SV=1	[more]
Q9LFP5	2.0e-154	58.24	Probable pectate lyase 19 OS=Arabidopsis thaliana OX=3702 GN=At5g15110 PE=2 SV=1	[more]
Q9M9S2	7.3e-149	60.71	Probable pectate lyase 3 OS=Arabidopsis thaliana OX=3702 GN=AT59 PE=2 SV=1	[more]

BLAST of Tan0014447 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008456459.1	7.5e-228	87.16	PREDICTED: pectate lyase-like [Cucumis melo]	[more]
XP_004146792.2	2.1e-225	86.50	pectate lyase [Cucumis sativus] >KAE8647292.1 hypothetical protein Csa_003655 [C...	[more]
XP_038886315.1	9.5e-223	85.55	pectate lyase-like [Benincasa hispida]	[more]
KAG7020735.1	2.3e-216	83.86	putative pectate lyase P59 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022951907.1	1.5e-215	83.37	pectate lyase-like [Cucurbita moschata]	[more]

BLAST of Tan0014447 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3C3Z5	3.7e-228	87.16	Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103496402 PE=3 SV=1	[more]
A0A6J1GIY2	7.1e-216	83.37	Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111454644 PE=3 SV=1	[more]
A0A6J1G495	1.6e-215	82.27	Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111450600 PE=3 SV=1	[more]
A0A6J1KG97	1.5e-213	81.65	Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111493968 PE=3 SV=1	[more]
A0A5A7UQS9	7.2e-208	91.42	Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold360G00930 ...	[more]

BLAST of Tan0014447 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G01270.1	7.4e-157	58.17	Pectate lyase family protein	[more]
AT5G15110.1	1.8e-155	58.03	Pectate lyase family protein	[more]
AT1G14420.1	5.2e-150	60.71	Pectate lyase family protein	[more]
AT2G02720.1	3.0e-142	56.42	Pectate lyase family protein	[more]
AT4G13710.1	8.2e-140	58.87	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR018082	AmbAllergen	PRINTS	PR00807	AMBALLERGEN	coord: 407..430 score: 40.08 coord: 231..252 score: 64.94 coord: 375..399 score: 51.24 coord: 333..352 score: 77.86 coord: 107..124 score: 57.94 coord: 131..156 score: 70.15 coord: 311..330 score: 78.1 coord: 167..183 score: 65.55
IPR002022	Pectate lyase	SMART	SM00656	amb_all	coord: 159..356 e-value: 8.6E-102 score: 354.2
IPR002022	Pectate lyase	PFAM	PF00544	Pectate_lyase_4	coord: 168..350 e-value: 2.5E-20 score: 73.0
IPR007524	Pectate lyase, N-terminal	PFAM	PF04431	Pec_lyase_N	coord: 23..74 e-value: 4.2E-20 score: 71.8
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 84..429 e-value: 4.6E-116 score: 389.5
None	No IPR available	PANTHER	PTHR31683:SF132	PECTATE LYASE	coord: 6..436
IPR045032	Pectin lyase family	PANTHER	PTHR31683	PECTATE LYASE 18-RELATED	coord: 6..436
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 58..406

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0014447.1	Tan0014447.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045490	pectin catabolic process
molecular_function	GO:0004556	alpha-amylase activity
molecular_function	GO:0103025	alpha-amylase activity (releasing maltohexaose)
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0030570	pectate lyase activity