Tan0012821 (gene) Snake gourd v1

Overview
NameTan0012821
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionprotein PIN-LIKES 2
LocationLG11: 18976498 .. 18978294 (-)
RNA-Seq ExpressionTan0012821
SyntenyTan0012821
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCTCCAGTGAGTTATCTCTCAGGTTTCTTTTTACTTTCTTCTCTATTTCGATCGAGCAGTTTCTGATTAGTTCAATTGCTTTCAATTTTGCTTACAAATTTCCAAAACCCCCGTTTAATCTTTCATTGCAATTTCTGGTTCCGTTTAGCTGATTAGCTCTGAAAATCCACTCCCATGTCCTGTCCTCTCTTTGTGGTTTTCGCTTTCAGATTTTGATTACAAATCTTTCAGTTTCTCGTTGATTCATCGCAATAAACAAACGATTGTTTGTTCTAGTAATTACCCAAAATCGAGATGGCCGATTACTCCAGTTACGTAGATCGAAATCGAGTGAGGTCGGGTACTGAAGATCTACTTGGTGCGATTATGCCATTGATGAAGCTTCTCTCCCTCACAGCAATAGGGTTATTACTTTCTCACCCAAAAATCCAAATGATTCCAAGAGCAACCCTAAGGCTCCTGAGCAAACTTGTTTTTGCCCTGTTCTTGCCCTGCCTCATCTTCACCTATCTGGGTCAGAGCATTACAATTGAGAACATTGCTAAATGGTGGTTCATCCCTGTAAATGTTTTGATCAGTACAGGTATTGGATGCCTTCTAGGGTTCTTGGTGGTGATTCTATGTCGTCCCCCTCCTCCGTTGGTTCGATTCACCATCATCTCGACCGCGTTCGGTAACACCGGAAATCTTCCTCTTGCCATTGTTAGTTCTGTGTGTCATACTGCTGATAACCCCTTTGGGCAACATTGTCACTCAAGAGGGGTTTCTTATGTTTCCTTTTGCCAATGGGTTTCTGTTATTCTTGCTTTGGAGTTTTATGAGATTGTTGAGGAAGGCACTGAGATTGAAGAATTGGTTGAAACTAATGATACTGGTAATGATGTAAGTAAGCCTCTCCTGATAGAAGCCGAATGGCCCGGGATTGAAGAAAAAGAAACTGAGCATTGTAAAGCACCCTTCATTGCTAGGATCTTCAATAGCATTTCAAATGTTTCCCAGTCTACACTTCCTGACTTTGATCACAACTCCAGAGAAAATAGTTCTCCTATGTACCCCGAGTCGATCAGGTGTTTGGCCGAACCGAGGGTTGTGAGGAAGATAAGAATTGTAGCTGAACAAACTCCTATACAGCACATTCTGCAGCCCCCAACCATCGCTTCTTTGTTAGCCATCATTGTTGGATTGGTGCCTCAAATCAAAGCATCCTTCTTTGGCGCCGATGCTCCGCTATCATTTATCTCTGATAGTTTAGAAATTTTAGCTGGTGCAATGGTGCCTTTCGTGATGCTAATCTTGGGCGGCATGCTTGCTGAGGGGCCTAATGAGTCGTCTACACTCGGCCTACGCACAACAATCGGCATAAGCATTGCAAGACTGTTGGTCCTTCCTGTACTAGGAATCGGGATCGTCGTAGCCGCCGATAAGCTGAACTTTCTAGTTCATGGTGATCCAATGTACAAATTTGTGCTTTTATTGCAGTATACAACACCAACTGCCATTTTGTTAGGAGCTATTGCCAGCTTGAGGGGATATGCAGTGAAGGAGGCCTCAGCTCTACTGTTTTGGGAACATGTATTTGCCCTCCTCTCCCTGACTCTCTATATTTTTGTTTACTTTAAGGTTGTGATATAAGTATGATTTTACTGCAAATTGTACAATCTGTATCAATGGCTGTTTCCTTCTCTTAGCAAGCATTCATTTTGGGAATAAATGCTCTTCCCTCTCTATTGTATCATACTTCTATAGCTTTGGTTGAAAACTCGTAGTATGTAAATTTCTTTATTCTAACCTAGAAA

mRNA sequence

TTCTCCAGTGAGTTATCTCTCAGTAATTACCCAAAATCGAGATGGCCGATTACTCCAGTTACGTAGATCGAAATCGAGTGAGGTCGGGTACTGAAGATCTACTTGGTGCGATTATGCCATTGATGAAGCTTCTCTCCCTCACAGCAATAGGGTTATTACTTTCTCACCCAAAAATCCAAATGATTCCAAGAGCAACCCTAAGGCTCCTGAGCAAACTTGTTTTTGCCCTGTTCTTGCCCTGCCTCATCTTCACCTATCTGGGTCAGAGCATTACAATTGAGAACATTGCTAAATGGTGGTTCATCCCTGTAAATGTTTTGATCAGTACAGGTATTGGATGCCTTCTAGGGTTCTTGGTGGTGATTCTATGTCGTCCCCCTCCTCCGTTGGTTCGATTCACCATCATCTCGACCGCGTTCGGTAACACCGGAAATCTTCCTCTTGCCATTGTTAGTTCTGTGTGTCATACTGCTGATAACCCCTTTGGGCAACATTGTCACTCAAGAGGGGTTTCTTATGTTTCCTTTTGCCAATGGGTTTCTGTTATTCTTGCTTTGGAGTTTTATGAGATTGTTGAGGAAGGCACTGAGATTGAAGAATTGGTTGAAACTAATGATACTGGTAATGATGTAAGTAAGCCTCTCCTGATAGAAGCCGAATGGCCCGGGATTGAAGAAAAAGAAACTGAGCATTGTAAAGCACCCTTCATTGCTAGGATCTTCAATAGCATTTCAAATGTTTCCCAGTCTACACTTCCTGACTTTGATCACAACTCCAGAGAAAATAGTTCTCCTATGTACCCCGAGTCGATCAGGTGTTTGGCCGAACCGAGGGTTGTGAGGAAGATAAGAATTGTAGCTGAACAAACTCCTATACAGCACATTCTGCAGCCCCCAACCATCGCTTCTTTGTTAGCCATCATTGTTGGATTGGTGCCTCAAATCAAAGCATCCTTCTTTGGCGCCGATGCTCCGCTATCATTTATCTCTGATAGTTTAGAAATTTTAGCTGGTGCAATGGTGCCTTTCGTGATGCTAATCTTGGGCGGCATGCTTGCTGAGGGGCCTAATGAGTCGTCTACACTCGGCCTACGCACAACAATCGGCATAAGCATTGCAAGACTGTTGGTCCTTCCTGTACTAGGAATCGGGATCGTCGTAGCCGCCGATAAGCTGAACTTTCTAGTTCATGGTGATCCAATGTACAAATTTGTGCTTTTATTGCAGTATACAACACCAACTGCCATTTTGTTAGGAGCTATTGCCAGCTTGAGGGGATATGCAGTGAAGGAGGCCTCAGCTCTACTGTTTTGGGAACATGTATTTGCCCTCCTCTCCCTGACTCTCTATATTTTTGTTTACTTTAAGGTTGTGATATAAGTATGATTTTACTGCAAATTGTACAATCTGTATCAATGGCTGTTTCCTTCTCTTAGCAAGCATTCATTTTGGGAATAAATGCTCTTCCCTCTCTATTGTATCATACTTCTATAGCTTTGGTTGAAAACTCGTAGTATGTAAATTTCTTTATTCTAACCTAGAAA

Coding sequence (CDS)

ATGGCCGATTACTCCAGTTACGTAGATCGAAATCGAGTGAGGTCGGGTACTGAAGATCTACTTGGTGCGATTATGCCATTGATGAAGCTTCTCTCCCTCACAGCAATAGGGTTATTACTTTCTCACCCAAAAATCCAAATGATTCCAAGAGCAACCCTAAGGCTCCTGAGCAAACTTGTTTTTGCCCTGTTCTTGCCCTGCCTCATCTTCACCTATCTGGGTCAGAGCATTACAATTGAGAACATTGCTAAATGGTGGTTCATCCCTGTAAATGTTTTGATCAGTACAGGTATTGGATGCCTTCTAGGGTTCTTGGTGGTGATTCTATGTCGTCCCCCTCCTCCGTTGGTTCGATTCACCATCATCTCGACCGCGTTCGGTAACACCGGAAATCTTCCTCTTGCCATTGTTAGTTCTGTGTGTCATACTGCTGATAACCCCTTTGGGCAACATTGTCACTCAAGAGGGGTTTCTTATGTTTCCTTTTGCCAATGGGTTTCTGTTATTCTTGCTTTGGAGTTTTATGAGATTGTTGAGGAAGGCACTGAGATTGAAGAATTGGTTGAAACTAATGATACTGGTAATGATGTAAGTAAGCCTCTCCTGATAGAAGCCGAATGGCCCGGGATTGAAGAAAAAGAAACTGAGCATTGTAAAGCACCCTTCATTGCTAGGATCTTCAATAGCATTTCAAATGTTTCCCAGTCTACACTTCCTGACTTTGATCACAACTCCAGAGAAAATAGTTCTCCTATGTACCCCGAGTCGATCAGGTGTTTGGCCGAACCGAGGGTTGTGAGGAAGATAAGAATTGTAGCTGAACAAACTCCTATACAGCACATTCTGCAGCCCCCAACCATCGCTTCTTTGTTAGCCATCATTGTTGGATTGGTGCCTCAAATCAAAGCATCCTTCTTTGGCGCCGATGCTCCGCTATCATTTATCTCTGATAGTTTAGAAATTTTAGCTGGTGCAATGGTGCCTTTCGTGATGCTAATCTTGGGCGGCATGCTTGCTGAGGGGCCTAATGAGTCGTCTACACTCGGCCTACGCACAACAATCGGCATAAGCATTGCAAGACTGTTGGTCCTTCCTGTACTAGGAATCGGGATCGTCGTAGCCGCCGATAAGCTGAACTTTCTAGTTCATGGTGATCCAATGTACAAATTTGTGCTTTTATTGCAGTATACAACACCAACTGCCATTTTGTTAGGAGCTATTGCCAGCTTGAGGGGATATGCAGTGAAGGAGGCCTCAGCTCTACTGTTTTGGGAACATGTATTTGCCCTCCTCTCCCTGACTCTCTATATTTTTGTTTACTTTAAGGTTGTGATATAA

Protein sequence

MADYSSYVDRNRVRSGTEDLLGAIMPLMKLLSLTAIGLLLSHPKIQMIPRATLRLLSKLVFALFLPCLIFTYLGQSITIENIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPPLVRFTIISTAFGNTGNLPLAIVSSVCHTADNPFGQHCHSRGVSYVSFCQWVSVILALEFYEIVEEGTEIEELVETNDTGNDVSKPLLIEAEWPGIEEKETEHCKAPFIARIFNSISNVSQSTLPDFDHNSRENSSPMYPESIRCLAEPRVVRKIRIVAEQTPIQHILQPPTIASLLAIIVGLVPQIKASFFGADAPLSFISDSLEILAGAMVPFVMLILGGMLAEGPNESSTLGLRTTIGISIARLLVLPVLGIGIVVAADKLNFLVHGDPMYKFVLLLQYTTPTAILLGAIASLRGYAVKEASALLFWEHVFALLSLTLYIFVYFKVVI
Homology
BLAST of Tan0012821 vs. ExPASy Swiss-Prot
Match: Q9C999 (Protein PIN-LIKES 2 OS=Arabidopsis thaliana OX=3702 GN=PILS2 PE=2 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 1.8e-166
Identity = 307/457 (67.18%), Postives = 372/457 (81.40%), Query Frame = 0

Query: 1   MADYSSYVDRNRVRSGTEDLLGAIMPLMKLLSLTAIGLLLSHPKIQMIPRATLRLLSKLV 60
           M+ +SS      V S   D+L  ++PL+KL+ LT IGLLL+HPK Q++PRAT RLLSKLV
Sbjct: 1   MSGFSS----GNVNSRVVDILSGVVPLLKLICLTVIGLLLAHPKTQLVPRATFRLLSKLV 60

Query: 61  FALFLPCLIFTYLGQSITIENIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPPLVRFT 120
           FALFLPCLIFT LG+SIT++NI +WWFIPVNVL+S  +G L+G+LVV++CRPPP   RFT
Sbjct: 61  FALFLPCLIFTELGESITLDNIVQWWFIPVNVLLSAVVGSLIGYLVVLICRPPPEFNRFT 120

Query: 121 IISTAFGNTGNLPLAIVSSVCHTADNPFGQHCHSRGVSYVSFCQWVSVILA--------- 180
           I+ TAFGNTGNL LAIVSSVCHT  NPFG +C+SRGVSYVSF QWV+VIL          
Sbjct: 121 IVMTAFGNTGNLLLAIVSSVCHTKTNPFGPNCNSRGVSYVSFAQWVAVILVYTVVYHMME 180

Query: 181 --LEFYEIV-EEGTEIEELVETNDTGNDVSKPLLIEAEWPGIEEKETEHCKAPFIARIFN 240
             LE+YE+V EEG EIEE+   N   +D S+PLL+EAEWPGIE+KETEHCK PFIAR+FN
Sbjct: 181 PPLEYYEVVEEEGVEIEEI---NVENHDASRPLLVEAEWPGIEDKETEHCKTPFIARVFN 240

Query: 241 SISNVSQSTLPDFDHNSRENSSPMYPESIRCLAEPRVVRKIRIVAEQTPIQHILQPPTIA 300
           SIS+ SQ++ P+ D           P SI+CLAEPRV+R+IR+VAEQTP++HILQPPTIA
Sbjct: 241 SISSFSQTSFPEVDLGGEYGGESSSPRSIQCLAEPRVMRRIRVVAEQTPVKHILQPPTIA 300

Query: 301 SLLAIIVGLVPQIKASFFGADAPLSFISDSLEILAGAMVPFVMLILGGMLAEGPNESSTL 360
           SLLAII+G VPQ+K+  FG DAPLSFI+DSL I+  AMVP VML+LGGML+EGPNE STL
Sbjct: 301 SLLAIIIGSVPQLKSVVFGYDAPLSFITDSLNIMGSAMVPSVMLVLGGMLSEGPNE-STL 360

Query: 361 GLRTTIGISIARLLVLPVLGIGIVVAADKLNFLVHGDPMYKFVLLLQYTTPTAILLGAIA 420
           GLRTTIGIS+ARLLVLP++GIGIV++ADKL  +   DPM+KFVLLLQY+TP+AILLGAIA
Sbjct: 361 GLRTTIGISVARLLVLPLVGIGIVMSADKLGLISSADPMFKFVLLLQYSTPSAILLGAIA 420

Query: 421 SLRGYAVKEASALLFWEHVFALLSLTLYIFVYFKVVI 446
           SLRGYAV+EASALLFW+H+FALLSLT YI ++FK+ +
Sbjct: 421 SLRGYAVREASALLFWQHIFALLSLTFYIVIFFKLTV 449

BLAST of Tan0012821 vs. ExPASy Swiss-Prot
Match: Q9LZN2 (Protein PIN-LIKES 6 OS=Arabidopsis thaliana OX=3702 GN=PILS6 PE=2 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 9.0e-70
Identity = 161/438 (36.76%), Postives = 254/438 (57.99%), Query Frame = 0

Query: 13  VRSGTEDLLG----AIMPLMKLLSLTAIGLLLSHPKIQMIPRATLRLLSKLVFALFLPCL 72
           V +G   +LG    A+MP+ K+ ++  +GLL++   + ++P +  +LL+ LVF+L LPCL
Sbjct: 17  VAAGGGSVLGTIKIAVMPIAKVFTMCFLGLLMASKYVNILPPSGRKLLNGLVFSLLLPCL 76

Query: 73  IFTYLGQSITIENIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPPLVRFTIISTAFGN 132
           IF+ LGQ++T++ + +WWFIPVNV++ T  G ++GF+V  + RPP P  +FTII    GN
Sbjct: 77  IFSQLGQAVTLQKMLQWWFIPVNVVLGTISGSIIGFIVASIVRPPYPYFKFTIIQIGVGN 136

Query: 133 TGNLPLAIVSSVCHTADNPFG--QHCHSRGVSYVSFCQWVSVILALEFYEIVEEGTEIEE 192
            GN+PL +++++C    NPFG  + C   G +Y+SF QWV  I+           T + +
Sbjct: 137 IGNVPLVLLAALCRDTSNPFGDSEKCSIDGTAYISFGQWVGAIILY---------TYVYQ 196

Query: 193 LVETNDTGNDVSKPLLIEAEWPGIEEKETEHCKAPFIARIFNSISNVSQSTLPDFDHNSR 252
           +      G D  +  L     P     E    + P + + F    + +Q  LP       
Sbjct: 197 MFAPPPEGFDAEEENLALKTLPVDAAPE----QVPLLTQNFPKDFSPTQDLLP------- 256

Query: 253 ENSSPMYPESIRCLAEPRVVRKIRIVAEQTPIQHILQPPTIASLLAIIVGLVPQIKASFF 312
                  P       + ++ +    + E+  ++ I+QP  +AS+LA+I+G +P  K   F
Sbjct: 257 --VQSTEPRGRGVSRKGKIAQIFVFLYEKLKLKQIVQPAIVASILAMILGAIPFTKKLIF 316

Query: 313 GADAPLSFISDSLEILAGAMVPFVMLILGGMLAEGPNESSTLGLRTTIGISIARLLVLPV 372
              APL F +DS  IL  AM+P ++L LGG L  GP  SS LG +TT  I I RL+++P 
Sbjct: 317 TNGAPLFFFTDSCMILGDAMIPCILLALGGNLINGPG-SSKLGFKTTAAIIIGRLVLVPP 376

Query: 373 LGIGIVVAADKLNFLVHGDPMYKFVLLLQYTTPTAILLGAIASLRGYAVKEASALLFWEH 432
           +G+GIV  ADKL FL   D M++FVLLLQ+T PT++L GA+A+LRG   +E++A+LFW H
Sbjct: 377 VGLGIVTVADKLGFLPADDKMFRFVLLLQHTMPTSVLSGAVANLRGCG-RESAAVLFWVH 430

Query: 433 VFALLSLTLYIFVYFKVV 445
           +FA+ S+  ++ +Y  ++
Sbjct: 437 IFAIFSMAGWMVLYINIL 430

BLAST of Tan0012821 vs. ExPASy Swiss-Prot
Match: Q9SHL8 (Protein PIN-LIKES 5 OS=Arabidopsis thaliana OX=3702 GN=PILS5 PE=2 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 4.2e-43
Identity = 126/425 (29.65%), Postives = 226/425 (53.18%), Query Frame = 0

Query: 23  AIMPLMKLLSLTAIGLLLSHPKIQMIPRATLRLLSKLVFALFLPCLIFTYLGQSITIENI 82
           A MP++++L ++ +G  ++  + ++ P      ++K+VF LF P L+F  L Q++T+E+I
Sbjct: 10  ASMPVIQVLFMSLVGAFMASDRCKLFPVEARNSMNKVVFVLFAPALMFANLAQTVTLEDI 69

Query: 83  AKWWFIPVNVLISTGIGCLLGFLVVILCRPPPPLVRFTIISTAFGNTGNLPLAIVSSVCH 142
             WWF+PVN+ ++  IG LLG+LVV + +PPP L    + + + GN GNLP+ +V ++C 
Sbjct: 70  ISWWFMPVNMGLTFLIGGLLGWLVVKILKPPPYLEGLIVATCSAGNMGNLPIILVPAICD 129

Query: 143 TADNPFGQH--CHSRGVSYVSFCQWVSVILALEFYEIVEEGTEIEELVETNDTGNDVSKP 202
              +PFG    C + G+SY SF       +AL  + I    T    L++           
Sbjct: 130 EDKSPFGNRSVCRTVGLSYASFS------MALGGFYI---WTYTFRLIK--------GSA 189

Query: 203 LLIEAEWPGIEEKETEHCKAPFIARIFNSISNVSQSTLPDFDHNSRENSSPMYPESIRCL 262
           + ++A    IEE E               I+  S ++  + DH +    +P   E+ + +
Sbjct: 190 MKVQA----IEESE--------------KIAIKSSNSDLEADHKTHLLGAPEDKEN-KVV 249

Query: 263 AEPRVVRKIRIVAEQTPIQHILQPPTIASLLAIIVGLVPQIKASFFGADAPLSFISDSLE 322
            E     +  +      ++ +L PPT+ +++  I G V  ++    G DAPL  +  + +
Sbjct: 250 KEKTGFWRKGVDFLHEILEELLAPPTLGAIIGFIFGAVRWLRNLIIGDDAPLRIVQSTAK 309

Query: 323 ILAGAMVPFVMLILGGMLAEGPNESSTLGLRTTIGISIARLLVLPVLGIGIVVAADKLNF 382
           +L    +P + +ILGG L +G   SS +     +GI   R + +P++GIGIV+ A  L F
Sbjct: 310 LLGDGTIPCMTIILGGNLIQG-LRSSAVKPMVVLGIVCVRYIAMPIIGIGIVLTAANLGF 369

Query: 383 LVHGDPMYKFVLLLQYTTPTAILLGAIASLRGYAVKEASALLFWEHVFALLSLTLYIFVY 442
           L   DP++++VL+LQ+T P A+ +G +  L   A  E S L+ W ++ A+L+LT++  ++
Sbjct: 370 L-PADPLFQYVLMLQFTLPPAMNIGTMTQLYNVAQDECSVLMLWTYLVAILALTVWSTIF 396

Query: 443 FKVVI 446
             +++
Sbjct: 430 LHLLV 396

BLAST of Tan0012821 vs. ExPASy Swiss-Prot
Match: F4HWB6 (Protein PIN-LIKES 1 OS=Arabidopsis thaliana OX=3702 GN=PILS1 PE=2 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 1.2e-42
Identity = 122/414 (29.47%), Postives = 206/414 (49.76%), Query Frame = 0

Query: 25  MPLMKLLSLTAIGLLLSHPKIQMIPRATLRLLSKLVFALFLPCLIFTYLGQSITIENIAK 84
           +P+ K+L +T IG  L+  ++ ++     + L+ +VF +F P L+ + L ++IT E++ K
Sbjct: 100 IPVAKILLITGIGFYLALDQVNILNHDARKQLNNIVFYVFSPSLVASSLSETITYESMVK 159

Query: 85  WWFIPVNVLISTGIGCLLGFLVVILCRPPPPLVRFTIISTAFGNTGNLPLAIVSSVCHTA 144
            WF+P+NVL++  IG  LG++V+ + +PP  L    +   A GN GN+PL I+ ++C+  
Sbjct: 160 MWFMPLNVLLTFIIGSFLGWIVIKITKPPSHLRGIIVGCCAAGNLGNMPLIIIPAICNEK 219

Query: 145 DNPFG--QHCHSRGVSYVSFCQWVSVILALEFYEIVEEGTEIEELVETNDTGNDVSKPLL 204
            +PFG  + C   G+ Y++    +  I                                 
Sbjct: 220 GSPFGDPESCEKFGLGYIALSMAIGAIYI------------------------------- 279

Query: 205 IEAEWPGIEEKETEHCKAPFIARIFNSISNVSQSTLPDFDHNSRENSSPMYPESIRCLAE 264
               W              ++  +   ++N +  T  +   ++    SP    + +    
Sbjct: 280 ----W-------------TYVYNLMRMLANPAGETAINSTSSTMPLISPKVEVAEQVGTW 339

Query: 265 PRVVRKIRIVAEQTPIQHILQPPTIASLLAIIVGLVPQIKASFFGADAPLSFISDSLEIL 324
            +V +++  VAE+  ++ I  P TIA+L+A+ VGL P ++    G  APL  I DS+ +L
Sbjct: 340 GKVKQRVCSVAEKINLRTIFAPSTIAALIALAVGLNPLLRKLLVGNTAPLRVIEDSVSLL 399

Query: 325 AGAMVPFVMLILGGMLAEGPNESSTLGLRTTIGISIARLLVLPVLGIGIVVAADKLNFLV 384
               +P + LI+GG L  G    S +     +G+ + R L+LP+LG+ IV  A  L  LV
Sbjct: 400 GDGAIPVLTLIVGGNLLNG-LRGSGINKSVIMGVVVVRYLLLPILGVFIVRGAHYLG-LV 459

Query: 385 HGDPMYKFVLLLQYTTPTAILLGAIASLRGYAVKEASALLFWEHVFALLSLTLY 437
             +P+Y+FVLLLQY  P A+ LG I  L G    E S +LFW +  A +SLT++
Sbjct: 460 TSEPLYQFVLLLQYVVPPAMNLGTITQLFGSGESECSVILFWSYALASVSLTVW 463

BLAST of Tan0012821 vs. ExPASy Swiss-Prot
Match: Q9C9K5 (Protein PIN-LIKES 3 OS=Arabidopsis thaliana OX=3702 GN=PILS3 PE=2 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 2.7e-42
Identity = 123/414 (29.71%), Postives = 218/414 (52.66%), Query Frame = 0

Query: 26  PLMKLLSLTAIGLLLSHPKIQMIPRATLRLLSKLVFALFLPCLIFTYLGQSITIENIAKW 85
           P++++L +T++G  ++   + ++     + L+ +VF +F P LI + L  S+T E++ K 
Sbjct: 14  PVVEILLITSVGFYMALDGVNLLGHDARKYLNNIVFYVFSPSLIGSRLADSVTYESLVKM 73

Query: 86  WFIPVNVLISTGIGCLLGFLVVILCRPPPPLVRFTIISTAFGNTGNLPLAIVSSVCHTAD 145
           WF+PVNVL++  IG LLG++V+++ +PP  L    +   A GN GN+PL I+ +VC    
Sbjct: 74  WFMPVNVLLTFIIGSLLGWIVIVITKPPSHLRGLILGCCAAGNLGNMPLIIIPAVCKEKG 133

Query: 146 NPFG--QHCHSRGVSYVSFCQWV-SVILALEFYEIVEEGTEIEELVETNDTGNDVSKPLL 205
            PFG  + C   G+ YV+    + S+ +    Y ++         V +N   + V  P  
Sbjct: 134 GPFGDPESCQKYGMGYVALSMAMGSIYIWTYVYNLMR--------VLSN---SPVETP-- 193

Query: 206 IEAEWPGIEEKETEHCKAPFIARIFNSISNVSQSTLPDFDHNSRENSSPMYPESIRCLAE 265
                P +E                   SN     +P       EN+        +    
Sbjct: 194 -----PSVE-------------------SNYDSYKVPLISSKEEENNQ-------KAGRW 253

Query: 266 PRVVRKIRIVAEQTPIQHILQPPTIASLLAIIVGLVPQIKASFFGADAPLSFISDSLEIL 325
            +V R++  ++++  ++ I  P TIA+++A+++GL+  ++    G +APL  + DS+ ++
Sbjct: 254 EKVKRRLVSLSQKVNLKTIFAPSTIAAMIALVIGLITPLRKLIIGTEAPLRVLQDSVTLV 313

Query: 326 AGAMVPFVMLILGGMLAEGPNESSTLGLRTTIGISIARLLVLPVLGIGIVVAADKLNFLV 385
               VP + +I+GG L +G   SS + + + IG+ +AR ++LP+ G+ IV  A KL+ LV
Sbjct: 314 GDGAVPAMTMIIGGNLLKG-LRSSGMKMSSIIGVLVARYVLLPMSGVLIVRGAYKLD-LV 373

Query: 386 HGDPMYKFVLLLQYTTPTAILLGAIASLRGYAVKEASALLFWEHVFALLSLTLY 437
             +P+Y+FVLLLQY  P A+ LG I  L G    E S ++ W +  A ++LT++
Sbjct: 374 TSEPLYQFVLLLQYAVPPAMNLGTITQLFGTGESECSVIMLWTYSLASIALTVW 381

BLAST of Tan0012821 vs. NCBI nr
Match: XP_038875937.1 (protein PIN-LIKES 2 [Benincasa hispida])

HSP 1 Score: 773.1 bits (1995), Expect = 1.3e-219
Identity = 413/457 (90.37%), Postives = 426/457 (93.22%), Query Frame = 0

Query: 1   MADY-SSYVDRNRVRSGTEDLLGAIMPLMKLLSLTAIGLLLSHPKIQMIPRATLRLLSKL 60
           MADY S  VDRN VRS +EDLL AIMPLMKLLSLTAIGLLLSHPKIQMIPR TLRLLSKL
Sbjct: 1   MADYFSVLVDRNGVRSSSEDLLTAIMPLMKLLSLTAIGLLLSHPKIQMIPRETLRLLSKL 60

Query: 61  VFALFLPCLIFTYLGQSITIENIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPPLVRF 120
           VFALFLPCLIFTYLG+SIT+ENIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPPLVRF
Sbjct: 61  VFALFLPCLIFTYLGESITLENIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPPLVRF 120

Query: 121 TIISTAFGNTGNLPLAIVSSVCHTADNPFGQHCHSRGVSYVSFCQWVSVILA-------- 180
           TIISTAFGNTGNLPLAIVSSVCHTADNPFG+HCHSRGVSYVSFCQWVSVILA        
Sbjct: 121 TIISTAFGNTGNLPLAIVSSVCHTADNPFGKHCHSRGVSYVSFCQWVSVILAYTLVYHMM 180

Query: 181 ---LEFYEIVEEGTEIEELVETNDTGNDVSKPLLIEAEWPGIEEKETEHCKAPFIARIFN 240
              LEFYEIVEEGTEIEELVETND  NDVSKPLL+EAEWPGIEEKETEHCKAPFIARIFN
Sbjct: 181 EPPLEFYEIVEEGTEIEELVETND--NDVSKPLLMEAEWPGIEEKETEHCKAPFIARIFN 240

Query: 241 SISNVSQSTLPDFDHNSRENSSPMYPESIRCLAEPRVVRKIRIVAEQTPIQHILQPPTIA 300
           SISNVSQS+ PD DH+   +S+P YPESIRCLAEPRVVRKIRIVAEQTPIQHILQPPTIA
Sbjct: 241 SISNVSQSSFPDLDHSRDSSSTPTYPESIRCLAEPRVVRKIRIVAEQTPIQHILQPPTIA 300

Query: 301 SLLAIIVGLVPQIKASFFGADAPLSFISDSLEILAGAMVPFVMLILGGMLAEGPNESSTL 360
           SLLAIIVGLVPQIKA FFGADAPL FIS+SLEI AGAMVPFVMLILGGMLAEGPNESSTL
Sbjct: 301 SLLAIIVGLVPQIKAFFFGADAPLLFISNSLEIFAGAMVPFVMLILGGMLAEGPNESSTL 360

Query: 361 GLRTTIGISIARLLVLPVLGIGIVVAADKLNFLVHGDPMYKFVLLLQYTTPTAILLGAIA 420
           GLRTTIGI IARLLVLPVLGIGIVVAADKLNFLVHGDPMYKFVLLLQYTTPTAILLGA+A
Sbjct: 361 GLRTTIGICIARLLVLPVLGIGIVVAADKLNFLVHGDPMYKFVLLLQYTTPTAILLGALA 420

Query: 421 SLRGYAVKEASALLFWEHVFALLSLTLYIFVYFKVVI 446
           SLRGYAV+EASALLFWEHVFALLSL+LYIFVYFK VI
Sbjct: 421 SLRGYAVREASALLFWEHVFALLSLSLYIFVYFKAVI 455

BLAST of Tan0012821 vs. NCBI nr
Match: XP_023521529.1 (protein PIN-LIKES 2-like [Cucurbita pepo subsp. pepo] >XP_023521530.1 protein PIN-LIKES 2-like [Cucurbita pepo subsp. pepo] >XP_023551435.1 protein PIN-LIKES 2-like [Cucurbita pepo subsp. pepo] >XP_023551436.1 protein PIN-LIKES 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 766.9 bits (1979), Expect = 9.4e-218
Identity = 401/456 (87.94%), Postives = 425/456 (93.20%), Query Frame = 0

Query: 1   MADYSSYVDRNRVRSGTEDLLGAIMPLMKLLSLTAIGLLLSHPKIQMIPRATLRLLSKLV 60
           MADY S  DRN VRS +EDLL AIMPLMKLLSLTAIGLLL+HPKIQMIPRATLRLLSKLV
Sbjct: 1   MADYFSVGDRNAVRSSSEDLLSAIMPLMKLLSLTAIGLLLAHPKIQMIPRATLRLLSKLV 60

Query: 61  FALFLPCLIFTYLGQSITIENIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPPLVRFT 120
           FALFLPCLIFTYLG+SIT++NIAKWWFIPVNV+ISTGIGC LGFLVVILCRPPP L+RFT
Sbjct: 61  FALFLPCLIFTYLGESITLDNIAKWWFIPVNVVISTGIGCFLGFLVVILCRPPPHLIRFT 120

Query: 121 IISTAFGNTGNLPLAIVSSVCHTADNPFGQHCHSRGVSYVSFCQWVSVILA--------- 180
           IIST FGNTGNLPLAIVSSVCHTADNPFG HCHSRGVSYVSFCQWVSVILA         
Sbjct: 121 IISTGFGNTGNLPLAIVSSVCHTADNPFGPHCHSRGVSYVSFCQWVSVILAYTLVYHMME 180

Query: 181 --LEFYEIVEEGTEIEELVETNDTGNDVSKPLLIEAEWPGIEEKETEHCKAPFIARIFNS 240
             +EFYEIVEEGTEIEELVE+NDT NDVSKPLL+EAEWPGIEEKETE+CKAPFIARIF S
Sbjct: 181 PPMEFYEIVEEGTEIEELVESNDTDNDVSKPLLMEAEWPGIEEKETENCKAPFIARIFKS 240

Query: 241 ISNVSQSTLPDFDHNSRENSSPMYPESIRCLAEPRVVRKIRIVAEQTPIQHILQPPTIAS 300
           ISNVSQST PD DH+   +++P YPESIRCLAEPRVVRKIRIVAEQTPIQHILQPPTIAS
Sbjct: 241 ISNVSQSTFPDLDHSRDSSTTPTYPESIRCLAEPRVVRKIRIVAEQTPIQHILQPPTIAS 300

Query: 301 LLAIIVGLVPQIKASFFGADAPLSFISDSLEILAGAMVPFVMLILGGMLAEGPNESSTLG 360
           LLAIIVGLVPQIKA FFG++APL FIS+SLEILAGAMVPFVMLILGGMLAEGPNESSTLG
Sbjct: 301 LLAIIVGLVPQIKACFFGSEAPLLFISNSLEILAGAMVPFVMLILGGMLAEGPNESSTLG 360

Query: 361 LRTTIGISIARLLVLPVLGIGIVVAADKLNFLVHGDPMYKFVLLLQYTTPTAILLGAIAS 420
           LRTTIGISIARLLVLPVLGIGI+VAADKLNFLVHGDPMYKFV+LLQYTTPTAIL+GAIAS
Sbjct: 361 LRTTIGISIARLLVLPVLGIGIIVAADKLNFLVHGDPMYKFVVLLQYTTPTAILVGAIAS 420

Query: 421 LRGYAVKEASALLFWEHVFALLSLTLYIFVYFKVVI 446
           LRGYAV+EASALLFWEHVFALLSL+LYIFVYFKVV+
Sbjct: 421 LRGYAVREASALLFWEHVFALLSLSLYIFVYFKVVL 456

BLAST of Tan0012821 vs. NCBI nr
Match: XP_022939090.1 (protein PIN-LIKES 2 [Cucurbita moschata] >XP_022939092.1 protein PIN-LIKES 2 [Cucurbita moschata] >KAG7016758.1 Protein PIN-LIKES 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 759.2 bits (1959), Expect = 2.0e-215
Identity = 400/456 (87.72%), Postives = 424/456 (92.98%), Query Frame = 0

Query: 1   MADYSSYVDRNRVRSGTEDLLGAIMPLMKLLSLTAIGLLLSHPKIQMIPRATLRLLSKLV 60
           MADY S  DRN VRS +EDLL AIMPLMKLLSLTAIGLLL+HPKIQMIPRATLRLLSKLV
Sbjct: 1   MADYFSVGDRNAVRSSSEDLLSAIMPLMKLLSLTAIGLLLAHPKIQMIPRATLRLLSKLV 60

Query: 61  FALFLPCLIFTYLGQSITIENIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPPLVRFT 120
           FALFLPCLIFTYLG+SIT++NIAKWWFIPVNV+ISTGIGC LGFLVVILCRPPP L+RFT
Sbjct: 61  FALFLPCLIFTYLGESITLDNIAKWWFIPVNVVISTGIGCFLGFLVVILCRPPPHLIRFT 120

Query: 121 IISTAFGNTGNLPLAIVSSVCHTADNPFGQHCHSRGVSYVSFCQWVSVILA--------- 180
           IIST FGNTGNLPLAIVSSVCHTADNPFG HCHSRGVSYVSFCQWVSVILA         
Sbjct: 121 IISTGFGNTGNLPLAIVSSVCHTADNPFGPHCHSRGVSYVSFCQWVSVILAYTLVYHMME 180

Query: 181 --LEFYEIVEEGTEIEELVETNDTGNDVSKPLLIEAEWPGIEEKETEHCKAPFIARIFNS 240
             +EFYEIVEEGTEIEELVE++D  NDVSKPLLIEAEWPGIEEKETE+CKAPFIARIF S
Sbjct: 181 PPMEFYEIVEEGTEIEELVESSD--NDVSKPLLIEAEWPGIEEKETENCKAPFIARIFKS 240

Query: 241 ISNVSQSTLPDFDHNSRENSSPMYPESIRCLAEPRVVRKIRIVAEQTPIQHILQPPTIAS 300
           ISNVSQST PD DH+   +++P YPESIRCLAEPRVVRKIRIVAEQTPIQHILQPPTIAS
Sbjct: 241 ISNVSQSTFPDLDHSRDSSTTPTYPESIRCLAEPRVVRKIRIVAEQTPIQHILQPPTIAS 300

Query: 301 LLAIIVGLVPQIKASFFGADAPLSFISDSLEILAGAMVPFVMLILGGMLAEGPNESSTLG 360
           LLAIIVGLVPQIKA FFG++APL FIS+SLEILAGAMVPFVMLILGGMLAEGPNESSTLG
Sbjct: 301 LLAIIVGLVPQIKACFFGSEAPLLFISNSLEILAGAMVPFVMLILGGMLAEGPNESSTLG 360

Query: 361 LRTTIGISIARLLVLPVLGIGIVVAADKLNFLVHGDPMYKFVLLLQYTTPTAILLGAIAS 420
           LRTTIGISIARLLVLPVLGIGI+VAADKLNFLVHGDPMYKFV+LLQYTTPTAIL+GAIAS
Sbjct: 361 LRTTIGISIARLLVLPVLGIGIIVAADKLNFLVHGDPMYKFVVLLQYTTPTAILVGAIAS 420

Query: 421 LRGYAVKEASALLFWEHVFALLSLTLYIFVYFKVVI 446
           LRGYAV+EASALLFWEHVFALLSL+LYIFVYFKVV+
Sbjct: 421 LRGYAVREASALLFWEHVFALLSLSLYIFVYFKVVL 454

BLAST of Tan0012821 vs. NCBI nr
Match: KAG6579244.1 (Protein PIN-LIKES 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 759.2 bits (1959), Expect = 2.0e-215
Identity = 400/456 (87.72%), Postives = 424/456 (92.98%), Query Frame = 0

Query: 1   MADYSSYVDRNRVRSGTEDLLGAIMPLMKLLSLTAIGLLLSHPKIQMIPRATLRLLSKLV 60
           MADY S  DRN VRS +EDLL AIMPLMKLLSLTAIGLLL+HPKIQMIPRATLRLLSKLV
Sbjct: 43  MADYFSVGDRNAVRSSSEDLLSAIMPLMKLLSLTAIGLLLAHPKIQMIPRATLRLLSKLV 102

Query: 61  FALFLPCLIFTYLGQSITIENIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPPLVRFT 120
           FALFLPCLIFTYLG+SIT++NIAKWWFIPVNV+ISTGIGC LGFLVVILCRPPP L+RFT
Sbjct: 103 FALFLPCLIFTYLGESITLDNIAKWWFIPVNVVISTGIGCFLGFLVVILCRPPPHLIRFT 162

Query: 121 IISTAFGNTGNLPLAIVSSVCHTADNPFGQHCHSRGVSYVSFCQWVSVILA--------- 180
           IIST FGNTGNLPLAIVSSVCHTADNPFG HCHSRGVSYVSFCQWVSVILA         
Sbjct: 163 IISTGFGNTGNLPLAIVSSVCHTADNPFGPHCHSRGVSYVSFCQWVSVILAYTLVYHMME 222

Query: 181 --LEFYEIVEEGTEIEELVETNDTGNDVSKPLLIEAEWPGIEEKETEHCKAPFIARIFNS 240
             +EFYEIVEEGTEIEELVE++D  NDVSKPLLIEAEWPGIEEKETE+CKAPFIARIF S
Sbjct: 223 PPMEFYEIVEEGTEIEELVESSD--NDVSKPLLIEAEWPGIEEKETENCKAPFIARIFKS 282

Query: 241 ISNVSQSTLPDFDHNSRENSSPMYPESIRCLAEPRVVRKIRIVAEQTPIQHILQPPTIAS 300
           ISNVSQST PD DH+   +++P YPESIRCLAEPRVVRKIRIVAEQTPIQHILQPPTIAS
Sbjct: 283 ISNVSQSTFPDLDHSRDSSTTPTYPESIRCLAEPRVVRKIRIVAEQTPIQHILQPPTIAS 342

Query: 301 LLAIIVGLVPQIKASFFGADAPLSFISDSLEILAGAMVPFVMLILGGMLAEGPNESSTLG 360
           LLAIIVGLVPQIKA FFG++APL FIS+SLEILAGAMVPFVMLILGGMLAEGPNESSTLG
Sbjct: 343 LLAIIVGLVPQIKACFFGSEAPLLFISNSLEILAGAMVPFVMLILGGMLAEGPNESSTLG 402

Query: 361 LRTTIGISIARLLVLPVLGIGIVVAADKLNFLVHGDPMYKFVLLLQYTTPTAILLGAIAS 420
           LRTTIGISIARLLVLPVLGIGI+VAADKLNFLVHGDPMYKFV+LLQYTTPTAIL+GAIAS
Sbjct: 403 LRTTIGISIARLLVLPVLGIGIIVAADKLNFLVHGDPMYKFVVLLQYTTPTAILVGAIAS 462

Query: 421 LRGYAVKEASALLFWEHVFALLSLTLYIFVYFKVVI 446
           LRGYAV+EASALLFWEHVFALLSL+LYIFVYFKVV+
Sbjct: 463 LRGYAVREASALLFWEHVFALLSLSLYIFVYFKVVL 496

BLAST of Tan0012821 vs. NCBI nr
Match: XP_022993212.1 (protein PIN-LIKES 2 [Cucurbita maxima] >XP_022993213.1 protein PIN-LIKES 2 [Cucurbita maxima])

HSP 1 Score: 758.1 bits (1956), Expect = 4.4e-215
Identity = 399/456 (87.50%), Postives = 424/456 (92.98%), Query Frame = 0

Query: 1   MADYSSYVDRNRVRSGTEDLLGAIMPLMKLLSLTAIGLLLSHPKIQMIPRATLRLLSKLV 60
           MADY S  D+N VRS +EDLL AIMPLMKLLSLTAIGLLL+HPKIQMIPRATLRLLSKLV
Sbjct: 1   MADYFSVGDQNEVRSSSEDLLSAIMPLMKLLSLTAIGLLLAHPKIQMIPRATLRLLSKLV 60

Query: 61  FALFLPCLIFTYLGQSITIENIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPPLVRFT 120
           FALFLPCLIFTYLG+SIT++NIAKWWFIPVNV+ISTGIGC LGFLVVILCRPPP L+RFT
Sbjct: 61  FALFLPCLIFTYLGESITLDNIAKWWFIPVNVVISTGIGCFLGFLVVILCRPPPHLIRFT 120

Query: 121 IISTAFGNTGNLPLAIVSSVCHTADNPFGQHCHSRGVSYVSFCQWVSVILA--------- 180
           IIST FGNTGNLPLAIVSSVCHTADNPFG HCHSRGVSYVSFCQWVSVILA         
Sbjct: 121 IISTGFGNTGNLPLAIVSSVCHTADNPFGPHCHSRGVSYVSFCQWVSVILAYTLVYHMME 180

Query: 181 --LEFYEIVEEGTEIEELVETNDTGNDVSKPLLIEAEWPGIEEKETEHCKAPFIARIFNS 240
             +EFYEIVEEGTEIEELVE++D  NDVSKPLLIEAEWPGIEEKETE+CKAPFIARIF S
Sbjct: 181 PPMEFYEIVEEGTEIEELVESSD--NDVSKPLLIEAEWPGIEEKETENCKAPFIARIFKS 240

Query: 241 ISNVSQSTLPDFDHNSRENSSPMYPESIRCLAEPRVVRKIRIVAEQTPIQHILQPPTIAS 300
           ISNVSQST PD DH+   +++P YPESIRCLAEPRVVRKIRIVAEQTPIQHILQPPTIAS
Sbjct: 241 ISNVSQSTFPDLDHSRDSSTTPTYPESIRCLAEPRVVRKIRIVAEQTPIQHILQPPTIAS 300

Query: 301 LLAIIVGLVPQIKASFFGADAPLSFISDSLEILAGAMVPFVMLILGGMLAEGPNESSTLG 360
           LLAIIVGLVPQIKA FFG++APL FIS+SLEILAGAMVPFVMLILGGMLAEGPNESSTLG
Sbjct: 301 LLAIIVGLVPQIKACFFGSEAPLLFISNSLEILAGAMVPFVMLILGGMLAEGPNESSTLG 360

Query: 361 LRTTIGISIARLLVLPVLGIGIVVAADKLNFLVHGDPMYKFVLLLQYTTPTAILLGAIAS 420
           LRTTIGISIARLLVLPVLGIGI+VAADKLNFLVHGDPMYKFV+LLQYTTPTAIL+GAIAS
Sbjct: 361 LRTTIGISIARLLVLPVLGIGIIVAADKLNFLVHGDPMYKFVVLLQYTTPTAILVGAIAS 420

Query: 421 LRGYAVKEASALLFWEHVFALLSLTLYIFVYFKVVI 446
           LRGYAV+EASALLFWEHVFALLSL+LYIFVYFKVV+
Sbjct: 421 LRGYAVREASALLFWEHVFALLSLSLYIFVYFKVVL 454

BLAST of Tan0012821 vs. ExPASy TrEMBL
Match: A0A6J1FFU8 (protein PIN-LIKES 2 OS=Cucurbita moschata OX=3662 GN=LOC111445095 PE=4 SV=1)

HSP 1 Score: 759.2 bits (1959), Expect = 9.5e-216
Identity = 400/456 (87.72%), Postives = 424/456 (92.98%), Query Frame = 0

Query: 1   MADYSSYVDRNRVRSGTEDLLGAIMPLMKLLSLTAIGLLLSHPKIQMIPRATLRLLSKLV 60
           MADY S  DRN VRS +EDLL AIMPLMKLLSLTAIGLLL+HPKIQMIPRATLRLLSKLV
Sbjct: 1   MADYFSVGDRNAVRSSSEDLLSAIMPLMKLLSLTAIGLLLAHPKIQMIPRATLRLLSKLV 60

Query: 61  FALFLPCLIFTYLGQSITIENIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPPLVRFT 120
           FALFLPCLIFTYLG+SIT++NIAKWWFIPVNV+ISTGIGC LGFLVVILCRPPP L+RFT
Sbjct: 61  FALFLPCLIFTYLGESITLDNIAKWWFIPVNVVISTGIGCFLGFLVVILCRPPPHLIRFT 120

Query: 121 IISTAFGNTGNLPLAIVSSVCHTADNPFGQHCHSRGVSYVSFCQWVSVILA--------- 180
           IIST FGNTGNLPLAIVSSVCHTADNPFG HCHSRGVSYVSFCQWVSVILA         
Sbjct: 121 IISTGFGNTGNLPLAIVSSVCHTADNPFGPHCHSRGVSYVSFCQWVSVILAYTLVYHMME 180

Query: 181 --LEFYEIVEEGTEIEELVETNDTGNDVSKPLLIEAEWPGIEEKETEHCKAPFIARIFNS 240
             +EFYEIVEEGTEIEELVE++D  NDVSKPLLIEAEWPGIEEKETE+CKAPFIARIF S
Sbjct: 181 PPMEFYEIVEEGTEIEELVESSD--NDVSKPLLIEAEWPGIEEKETENCKAPFIARIFKS 240

Query: 241 ISNVSQSTLPDFDHNSRENSSPMYPESIRCLAEPRVVRKIRIVAEQTPIQHILQPPTIAS 300
           ISNVSQST PD DH+   +++P YPESIRCLAEPRVVRKIRIVAEQTPIQHILQPPTIAS
Sbjct: 241 ISNVSQSTFPDLDHSRDSSTTPTYPESIRCLAEPRVVRKIRIVAEQTPIQHILQPPTIAS 300

Query: 301 LLAIIVGLVPQIKASFFGADAPLSFISDSLEILAGAMVPFVMLILGGMLAEGPNESSTLG 360
           LLAIIVGLVPQIKA FFG++APL FIS+SLEILAGAMVPFVMLILGGMLAEGPNESSTLG
Sbjct: 301 LLAIIVGLVPQIKACFFGSEAPLLFISNSLEILAGAMVPFVMLILGGMLAEGPNESSTLG 360

Query: 361 LRTTIGISIARLLVLPVLGIGIVVAADKLNFLVHGDPMYKFVLLLQYTTPTAILLGAIAS 420
           LRTTIGISIARLLVLPVLGIGI+VAADKLNFLVHGDPMYKFV+LLQYTTPTAIL+GAIAS
Sbjct: 361 LRTTIGISIARLLVLPVLGIGIIVAADKLNFLVHGDPMYKFVVLLQYTTPTAILVGAIAS 420

Query: 421 LRGYAVKEASALLFWEHVFALLSLTLYIFVYFKVVI 446
           LRGYAV+EASALLFWEHVFALLSL+LYIFVYFKVV+
Sbjct: 421 LRGYAVREASALLFWEHVFALLSLSLYIFVYFKVVL 454

BLAST of Tan0012821 vs. ExPASy TrEMBL
Match: A0A6J1JS57 (protein PIN-LIKES 2 OS=Cucurbita maxima OX=3661 GN=LOC111489301 PE=4 SV=1)

HSP 1 Score: 758.1 bits (1956), Expect = 2.1e-215
Identity = 399/456 (87.50%), Postives = 424/456 (92.98%), Query Frame = 0

Query: 1   MADYSSYVDRNRVRSGTEDLLGAIMPLMKLLSLTAIGLLLSHPKIQMIPRATLRLLSKLV 60
           MADY S  D+N VRS +EDLL AIMPLMKLLSLTAIGLLL+HPKIQMIPRATLRLLSKLV
Sbjct: 1   MADYFSVGDQNEVRSSSEDLLSAIMPLMKLLSLTAIGLLLAHPKIQMIPRATLRLLSKLV 60

Query: 61  FALFLPCLIFTYLGQSITIENIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPPLVRFT 120
           FALFLPCLIFTYLG+SIT++NIAKWWFIPVNV+ISTGIGC LGFLVVILCRPPP L+RFT
Sbjct: 61  FALFLPCLIFTYLGESITLDNIAKWWFIPVNVVISTGIGCFLGFLVVILCRPPPHLIRFT 120

Query: 121 IISTAFGNTGNLPLAIVSSVCHTADNPFGQHCHSRGVSYVSFCQWVSVILA--------- 180
           IIST FGNTGNLPLAIVSSVCHTADNPFG HCHSRGVSYVSFCQWVSVILA         
Sbjct: 121 IISTGFGNTGNLPLAIVSSVCHTADNPFGPHCHSRGVSYVSFCQWVSVILAYTLVYHMME 180

Query: 181 --LEFYEIVEEGTEIEELVETNDTGNDVSKPLLIEAEWPGIEEKETEHCKAPFIARIFNS 240
             +EFYEIVEEGTEIEELVE++D  NDVSKPLLIEAEWPGIEEKETE+CKAPFIARIF S
Sbjct: 181 PPMEFYEIVEEGTEIEELVESSD--NDVSKPLLIEAEWPGIEEKETENCKAPFIARIFKS 240

Query: 241 ISNVSQSTLPDFDHNSRENSSPMYPESIRCLAEPRVVRKIRIVAEQTPIQHILQPPTIAS 300
           ISNVSQST PD DH+   +++P YPESIRCLAEPRVVRKIRIVAEQTPIQHILQPPTIAS
Sbjct: 241 ISNVSQSTFPDLDHSRDSSTTPTYPESIRCLAEPRVVRKIRIVAEQTPIQHILQPPTIAS 300

Query: 301 LLAIIVGLVPQIKASFFGADAPLSFISDSLEILAGAMVPFVMLILGGMLAEGPNESSTLG 360
           LLAIIVGLVPQIKA FFG++APL FIS+SLEILAGAMVPFVMLILGGMLAEGPNESSTLG
Sbjct: 301 LLAIIVGLVPQIKACFFGSEAPLLFISNSLEILAGAMVPFVMLILGGMLAEGPNESSTLG 360

Query: 361 LRTTIGISIARLLVLPVLGIGIVVAADKLNFLVHGDPMYKFVLLLQYTTPTAILLGAIAS 420
           LRTTIGISIARLLVLPVLGIGI+VAADKLNFLVHGDPMYKFV+LLQYTTPTAIL+GAIAS
Sbjct: 361 LRTTIGISIARLLVLPVLGIGIIVAADKLNFLVHGDPMYKFVVLLQYTTPTAILVGAIAS 420

Query: 421 LRGYAVKEASALLFWEHVFALLSLTLYIFVYFKVVI 446
           LRGYAV+EASALLFWEHVFALLSL+LYIFVYFKVV+
Sbjct: 421 LRGYAVREASALLFWEHVFALLSLSLYIFVYFKVVL 454

BLAST of Tan0012821 vs. ExPASy TrEMBL
Match: A0A6J1DDP0 (protein PIN-LIKES 2-like OS=Momordica charantia OX=3673 GN=LOC111020086 PE=4 SV=1)

HSP 1 Score: 745.3 bits (1923), Expect = 1.4e-211
Identity = 392/456 (85.96%), Postives = 420/456 (92.11%), Query Frame = 0

Query: 1   MADYSSYVDRNRVRSGTEDLLGAIMPLMKLLSLTAIGLLLSHPKIQMIPRATLRLLSKLV 60
           M DY S +DR+ VRS +EDLL AI PLMKLLSLTAIGLLLSHPK+QMIPRATLRLLSKLV
Sbjct: 1   MGDYFSVIDRSGVRSSSEDLLSAITPLMKLLSLTAIGLLLSHPKVQMIPRATLRLLSKLV 60

Query: 61  FALFLPCLIFTYLGQSITIENIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPPLVRFT 120
           FALFLPCLIFT+LG+SIT++NIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPP L+RFT
Sbjct: 61  FALFLPCLIFTHLGESITLQNIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPRLMRFT 120

Query: 121 IISTAFGNTGNLPLAIVSSVCHTADNPFGQHCHSRGVSYVSFCQWVSVILA--------- 180
           +ISTAFGNTGNLPLAIV SVCHTADNPFGQHCHSRGVSYVSFCQWVSVILA         
Sbjct: 121 VISTAFGNTGNLPLAIVGSVCHTADNPFGQHCHSRGVSYVSFCQWVSVILAYTLVYHMME 180

Query: 181 --LEFYEIVEEGTEIEELVETNDTGNDVSKPLLIEAEWPGIEEKETEHCKAPFIARIFNS 240
             LEFYEIVEEGTEIEEL + +D  ND SKPLLIEAEWPGIEEKETEHCKAPFIARIF S
Sbjct: 181 PPLEFYEIVEEGTEIEELEQISD--NDASKPLLIEAEWPGIEEKETEHCKAPFIARIFKS 240

Query: 241 ISNVSQSTLPDFDHNSRENSSPMYPESIRCLAEPRVVRKIRIVAEQTPIQHILQPPTIAS 300
           ISNVSQST PD DH SRE+S+P YPESIRCLAEPRVVRKIRIVA QTP++HILQPPTIAS
Sbjct: 241 ISNVSQSTFPDLDH-SRESSTPTYPESIRCLAEPRVVRKIRIVAVQTPLKHILQPPTIAS 300

Query: 301 LLAIIVGLVPQIKASFFGADAPLSFISDSLEILAGAMVPFVMLILGGMLAEGPNESSTLG 360
           LLAIIVGLVPQI+ASFFG DAPLSFISDSLEILA A+VPFVML+LGGMLAEGP+E STLG
Sbjct: 301 LLAIIVGLVPQIRASFFGPDAPLSFISDSLEILAAAVVPFVMLMLGGMLAEGPHELSTLG 360

Query: 361 LRTTIGISIARLLVLPVLGIGIVVAADKLNFLVHGDPMYKFVLLLQYTTPTAILLGAIAS 420
           LRTTIGIS+ARL VLP+LGIGIVV+ADKLNFLV GDPMYKFVLLLQYTTPTAILLGAIAS
Sbjct: 361 LRTTIGISVARLFVLPILGIGIVVSADKLNFLVDGDPMYKFVLLLQYTTPTAILLGAIAS 420

Query: 421 LRGYAVKEASALLFWEHVFALLSLTLYIFVYFKVVI 446
           LRGYAV+EASALLFWEH+FALLSL+LY+FVYFK+VI
Sbjct: 421 LRGYAVREASALLFWEHIFALLSLSLYLFVYFKIVI 453

BLAST of Tan0012821 vs. ExPASy TrEMBL
Match: A0A0A0KRM3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G571470 PE=4 SV=1)

HSP 1 Score: 738.8 bits (1906), Expect = 1.3e-209
Identity = 393/457 (86.00%), Postives = 418/457 (91.47%), Query Frame = 0

Query: 1   MADY-SSYVDRNRVRSGTEDLLGAIMPLMKLLSLTAIGLLLSHPKIQMIPRATLRLLSKL 60
           M DY S  VDRN V S T DLL AI+PLMKLLSLTAIGLLLSHPKIQMIPRATLRL+SKL
Sbjct: 1   MDDYFSVVVDRNEVGSSTGDLLTAIVPLMKLLSLTAIGLLLSHPKIQMIPRATLRLISKL 60

Query: 61  VFALFLPCLIFTYLGQSITIENIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPPLVRF 120
           VFALFLPCLIFT+LG++IT+ENIAKWWF+PVNVLISTGIGCLLGFLVVI+CRPPP LVRF
Sbjct: 61  VFALFLPCLIFTHLGENITLENIAKWWFVPVNVLISTGIGCLLGFLVVIICRPPPQLVRF 120

Query: 121 TIISTAFGNTGNLPLAIVSSVCHTADNPFGQHCHSRGVSYVSFCQWVSVILA-------- 180
           TIISTAFGNTGNLPLAIVSSVCHT DNPFG++CHS+GVSYVSFCQWVSVI+A        
Sbjct: 121 TIISTAFGNTGNLPLAIVSSVCHTDDNPFGKNCHSKGVSYVSFCQWVSVIIAYTLVYHMM 180

Query: 181 ---LEFYEIVEEGTEIEELVETNDTGNDVSKPLLIEAEWPGIEEKETEHCKAPFIARIFN 240
              LEFYEIVEEGTEIEELVE++D  NDVSKPLL+EAEWPGIEEKETEHCK PFIAR+FN
Sbjct: 181 EPPLEFYEIVEEGTEIEELVESDD--NDVSKPLLMEAEWPGIEEKETEHCKVPFIARVFN 240

Query: 241 SISNVSQSTLPDFDHNSRENSSPMYPESIRCLAEPRVVRKIRIVAEQTPIQHILQPPTIA 300
           SISNVSQST PD DH  R++S+  YPESI CLAEPRVVRKIRIVAEQTPIQHILQPPTIA
Sbjct: 241 SISNVSQSTFPDLDH--RDSSTSAYPESITCLAEPRVVRKIRIVAEQTPIQHILQPPTIA 300

Query: 301 SLLAIIVGLVPQIKASFFGADAPLSFISDSLEILAGAMVPFVMLILGGMLAEGPNESSTL 360
           SLLAIIVGLVPQIKA  FGADAPL FIS+S EI AGAMVPFVMLILGGMLAEGPNESSTL
Sbjct: 301 SLLAIIVGLVPQIKAVLFGADAPLLFISNSFEIFAGAMVPFVMLILGGMLAEGPNESSTL 360

Query: 361 GLRTTIGISIARLLVLPVLGIGIVVAADKLNFLVHGDPMYKFVLLLQYTTPTAILLGAIA 420
           GLRTTIGI +ARLLVLPVLGIGIVVAADKLNFLVHGDPMYKFVLLLQYTTPTAILLGA+A
Sbjct: 361 GLRTTIGICVARLLVLPVLGIGIVVAADKLNFLVHGDPMYKFVLLLQYTTPTAILLGALA 420

Query: 421 SLRGYAVKEASALLFWEHVFALLSLTLYIFVYFKVVI 446
           SLRGYAVKEASALLFWEH+FALLSL+LY+FVYFK VI
Sbjct: 421 SLRGYAVKEASALLFWEHIFALLSLSLYVFVYFKAVI 453

BLAST of Tan0012821 vs. ExPASy TrEMBL
Match: A0A5D3E4I5 (Protein PIN-LIKES 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold426G00240 PE=4 SV=1)

HSP 1 Score: 734.6 bits (1895), Expect = 2.5e-208
Identity = 392/458 (85.59%), Postives = 418/458 (91.27%), Query Frame = 0

Query: 1   MADY-SSYVDRNRVRSGTEDLLGAIMPLMKLLSLTAIGLLLSHPKIQMIPRATLRLLSKL 60
           M DY S  VD N VRS + DLL AI+PLMKLLSLTAIGLLLSHPKIQMIPRATLRLLSKL
Sbjct: 1   MDDYLSVVVDPNEVRSTSGDLLSAIVPLMKLLSLTAIGLLLSHPKIQMIPRATLRLLSKL 60

Query: 61  VFALFLPCLIFTYLGQSITIENIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPPLVRF 120
           VFALFLPCLIFT+LG++IT+ENIAKWWF+PVNVLISTGIGCLLGFLVVI+CRPPP LVRF
Sbjct: 61  VFALFLPCLIFTHLGENITLENIAKWWFVPVNVLISTGIGCLLGFLVVIICRPPPQLVRF 120

Query: 121 TIISTAFGNTGNLPLAIVSSVCHTADNPFGQHCHSRGVSYVSFCQWVSVILA-------- 180
           TIISTAFGNTGNLPLAIVSSVCHT DNPFG++CH++GVSYVSFCQWVSVI+A        
Sbjct: 121 TIISTAFGNTGNLPLAIVSSVCHTDDNPFGKNCHTKGVSYVSFCQWVSVIIAYTFVYHMM 180

Query: 181 ---LEFYEIVEEGTEIEELVETNDTGNDVSKPLLIEAEWPGIEEKETEHCKAPFIARIFN 240
              LEFYEIVEEGTEIEELVE++D  NDVSKPLL+EAEWPGIEEKETEHCKAPFIAR+FN
Sbjct: 181 EPPLEFYEIVEEGTEIEELVESDD--NDVSKPLLMEAEWPGIEEKETEHCKAPFIARVFN 240

Query: 241 SISNVSQSTLPDFDH-NSRENSSPMYPESIRCLAEPRVVRKIRIVAEQTPIQHILQPPTI 300
           SISNVSQST PD DH      S+  YPESIRCLAEPRVVRKIRIVAEQTPIQHILQPPTI
Sbjct: 241 SISNVSQSTFPDLDHPRDGSTSASAYPESIRCLAEPRVVRKIRIVAEQTPIQHILQPPTI 300

Query: 301 ASLLAIIVGLVPQIKASFFGADAPLSFISDSLEILAGAMVPFVMLILGGMLAEGPNESST 360
           ASLLAIIVGLVPQIKA  FGADAPL FIS+SLEI AGAMVPFVMLILGGMLAEGPNESST
Sbjct: 301 ASLLAIIVGLVPQIKAVLFGADAPLLFISNSLEIFAGAMVPFVMLILGGMLAEGPNESST 360

Query: 361 LGLRTTIGISIARLLVLPVLGIGIVVAADKLNFLVHGDPMYKFVLLLQYTTPTAILLGAI 420
           LG RTTIGI +ARLLVLPVLGIGIV+AADKLNFLV+GDPMYKFVLLLQYTTPTAILLGA+
Sbjct: 361 LGQRTTIGICVARLLVLPVLGIGIVIAADKLNFLVNGDPMYKFVLLLQYTTPTAILLGAL 420

Query: 421 ASLRGYAVKEASALLFWEHVFALLSLTLYIFVYFKVVI 446
           ASLRGYAVKEASALLFWEH+FALLSL+LY+FVYFKVVI
Sbjct: 421 ASLRGYAVKEASALLFWEHIFALLSLSLYVFVYFKVVI 456

BLAST of Tan0012821 vs. TAIR 10
Match: AT1G71090.1 (Auxin efflux carrier family protein )

HSP 1 Score: 587.0 bits (1512), Expect = 1.2e-167
Identity = 307/457 (67.18%), Postives = 372/457 (81.40%), Query Frame = 0

Query: 1   MADYSSYVDRNRVRSGTEDLLGAIMPLMKLLSLTAIGLLLSHPKIQMIPRATLRLLSKLV 60
           M+ +SS      V S   D+L  ++PL+KL+ LT IGLLL+HPK Q++PRAT RLLSKLV
Sbjct: 1   MSGFSS----GNVNSRVVDILSGVVPLLKLICLTVIGLLLAHPKTQLVPRATFRLLSKLV 60

Query: 61  FALFLPCLIFTYLGQSITIENIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPPLVRFT 120
           FALFLPCLIFT LG+SIT++NI +WWFIPVNVL+S  +G L+G+LVV++CRPPP   RFT
Sbjct: 61  FALFLPCLIFTELGESITLDNIVQWWFIPVNVLLSAVVGSLIGYLVVLICRPPPEFNRFT 120

Query: 121 IISTAFGNTGNLPLAIVSSVCHTADNPFGQHCHSRGVSYVSFCQWVSVILA--------- 180
           I+ TAFGNTGNL LAIVSSVCHT  NPFG +C+SRGVSYVSF QWV+VIL          
Sbjct: 121 IVMTAFGNTGNLLLAIVSSVCHTKTNPFGPNCNSRGVSYVSFAQWVAVILVYTVVYHMME 180

Query: 181 --LEFYEIV-EEGTEIEELVETNDTGNDVSKPLLIEAEWPGIEEKETEHCKAPFIARIFN 240
             LE+YE+V EEG EIEE+   N   +D S+PLL+EAEWPGIE+KETEHCK PFIAR+FN
Sbjct: 181 PPLEYYEVVEEEGVEIEEI---NVENHDASRPLLVEAEWPGIEDKETEHCKTPFIARVFN 240

Query: 241 SISNVSQSTLPDFDHNSRENSSPMYPESIRCLAEPRVVRKIRIVAEQTPIQHILQPPTIA 300
           SIS+ SQ++ P+ D           P SI+CLAEPRV+R+IR+VAEQTP++HILQPPTIA
Sbjct: 241 SISSFSQTSFPEVDLGGEYGGESSSPRSIQCLAEPRVMRRIRVVAEQTPVKHILQPPTIA 300

Query: 301 SLLAIIVGLVPQIKASFFGADAPLSFISDSLEILAGAMVPFVMLILGGMLAEGPNESSTL 360
           SLLAII+G VPQ+K+  FG DAPLSFI+DSL I+  AMVP VML+LGGML+EGPNE STL
Sbjct: 301 SLLAIIIGSVPQLKSVVFGYDAPLSFITDSLNIMGSAMVPSVMLVLGGMLSEGPNE-STL 360

Query: 361 GLRTTIGISIARLLVLPVLGIGIVVAADKLNFLVHGDPMYKFVLLLQYTTPTAILLGAIA 420
           GLRTTIGIS+ARLLVLP++GIGIV++ADKL  +   DPM+KFVLLLQY+TP+AILLGAIA
Sbjct: 361 GLRTTIGISVARLLVLPLVGIGIVMSADKLGLISSADPMFKFVLLLQYSTPSAILLGAIA 420

Query: 421 SLRGYAVKEASALLFWEHVFALLSLTLYIFVYFKVVI 446
           SLRGYAV+EASALLFW+H+FALLSLT YI ++FK+ +
Sbjct: 421 SLRGYAVREASALLFWQHIFALLSLTFYIVIFFKLTV 449

BLAST of Tan0012821 vs. TAIR 10
Match: AT5G01990.1 (Auxin efflux carrier family protein )

HSP 1 Score: 265.8 bits (678), Expect = 6.4e-71
Identity = 161/438 (36.76%), Postives = 254/438 (57.99%), Query Frame = 0

Query: 13  VRSGTEDLLG----AIMPLMKLLSLTAIGLLLSHPKIQMIPRATLRLLSKLVFALFLPCL 72
           V +G   +LG    A+MP+ K+ ++  +GLL++   + ++P +  +LL+ LVF+L LPCL
Sbjct: 17  VAAGGGSVLGTIKIAVMPIAKVFTMCFLGLLMASKYVNILPPSGRKLLNGLVFSLLLPCL 76

Query: 73  IFTYLGQSITIENIAKWWFIPVNVLISTGIGCLLGFLVVILCRPPPPLVRFTIISTAFGN 132
           IF+ LGQ++T++ + +WWFIPVNV++ T  G ++GF+V  + RPP P  +FTII    GN
Sbjct: 77  IFSQLGQAVTLQKMLQWWFIPVNVVLGTISGSIIGFIVASIVRPPYPYFKFTIIQIGVGN 136

Query: 133 TGNLPLAIVSSVCHTADNPFG--QHCHSRGVSYVSFCQWVSVILALEFYEIVEEGTEIEE 192
            GN+PL +++++C    NPFG  + C   G +Y+SF QWV  I+           T + +
Sbjct: 137 IGNVPLVLLAALCRDTSNPFGDSEKCSIDGTAYISFGQWVGAIILY---------TYVYQ 196

Query: 193 LVETNDTGNDVSKPLLIEAEWPGIEEKETEHCKAPFIARIFNSISNVSQSTLPDFDHNSR 252
           +      G D  +  L     P     E    + P + + F    + +Q  LP       
Sbjct: 197 MFAPPPEGFDAEEENLALKTLPVDAAPE----QVPLLTQNFPKDFSPTQDLLP------- 256

Query: 253 ENSSPMYPESIRCLAEPRVVRKIRIVAEQTPIQHILQPPTIASLLAIIVGLVPQIKASFF 312
                  P       + ++ +    + E+  ++ I+QP  +AS+LA+I+G +P  K   F
Sbjct: 257 --VQSTEPRGRGVSRKGKIAQIFVFLYEKLKLKQIVQPAIVASILAMILGAIPFTKKLIF 316

Query: 313 GADAPLSFISDSLEILAGAMVPFVMLILGGMLAEGPNESSTLGLRTTIGISIARLLVLPV 372
              APL F +DS  IL  AM+P ++L LGG L  GP  SS LG +TT  I I RL+++P 
Sbjct: 317 TNGAPLFFFTDSCMILGDAMIPCILLALGGNLINGPG-SSKLGFKTTAAIIIGRLVLVPP 376

Query: 373 LGIGIVVAADKLNFLVHGDPMYKFVLLLQYTTPTAILLGAIASLRGYAVKEASALLFWEH 432
           +G+GIV  ADKL FL   D M++FVLLLQ+T PT++L GA+A+LRG   +E++A+LFW H
Sbjct: 377 VGLGIVTVADKLGFLPADDKMFRFVLLLQHTMPTSVLSGAVANLRGCG-RESAAVLFWVH 430

Query: 433 VFALLSLTLYIFVYFKVV 445
           +FA+ S+  ++ +Y  ++
Sbjct: 437 IFAIFSMAGWMVLYINIL 430

BLAST of Tan0012821 vs. TAIR 10
Match: AT2G17500.1 (Auxin efflux carrier family protein )

HSP 1 Score: 177.2 bits (448), Expect = 3.0e-44
Identity = 126/425 (29.65%), Postives = 226/425 (53.18%), Query Frame = 0

Query: 23  AIMPLMKLLSLTAIGLLLSHPKIQMIPRATLRLLSKLVFALFLPCLIFTYLGQSITIENI 82
           A MP++++L ++ +G  ++  + ++ P      ++K+VF LF P L+F  L Q++T+E+I
Sbjct: 10  ASMPVIQVLFMSLVGAFMASDRCKLFPVEARNSMNKVVFVLFAPALMFANLAQTVTLEDI 69

Query: 83  AKWWFIPVNVLISTGIGCLLGFLVVILCRPPPPLVRFTIISTAFGNTGNLPLAIVSSVCH 142
             WWF+PVN+ ++  IG LLG+LVV + +PPP L    + + + GN GNLP+ +V ++C 
Sbjct: 70  ISWWFMPVNMGLTFLIGGLLGWLVVKILKPPPYLEGLIVATCSAGNMGNLPIILVPAICD 129

Query: 143 TADNPFGQH--CHSRGVSYVSFCQWVSVILALEFYEIVEEGTEIEELVETNDTGNDVSKP 202
              +PFG    C + G+SY SF       +AL  + I    T    L++           
Sbjct: 130 EDKSPFGNRSVCRTVGLSYASFS------MALGGFYI---WTYTFRLIK--------GSA 189

Query: 203 LLIEAEWPGIEEKETEHCKAPFIARIFNSISNVSQSTLPDFDHNSRENSSPMYPESIRCL 262
           + ++A    IEE E               I+  S ++  + DH +    +P   E+ + +
Sbjct: 190 MKVQA----IEESE--------------KIAIKSSNSDLEADHKTHLLGAPEDKEN-KVV 249

Query: 263 AEPRVVRKIRIVAEQTPIQHILQPPTIASLLAIIVGLVPQIKASFFGADAPLSFISDSLE 322
            E     +  +      ++ +L PPT+ +++  I G V  ++    G DAPL  +  + +
Sbjct: 250 KEKTGFWRKGVDFLHEILEELLAPPTLGAIIGFIFGAVRWLRNLIIGDDAPLRIVQSTAK 309

Query: 323 ILAGAMVPFVMLILGGMLAEGPNESSTLGLRTTIGISIARLLVLPVLGIGIVVAADKLNF 382
           +L    +P + +ILGG L +G   SS +     +GI   R + +P++GIGIV+ A  L F
Sbjct: 310 LLGDGTIPCMTIILGGNLIQG-LRSSAVKPMVVLGIVCVRYIAMPIIGIGIVLTAANLGF 369

Query: 383 LVHGDPMYKFVLLLQYTTPTAILLGAIASLRGYAVKEASALLFWEHVFALLSLTLYIFVY 442
           L   DP++++VL+LQ+T P A+ +G +  L   A  E S L+ W ++ A+L+LT++  ++
Sbjct: 370 L-PADPLFQYVLMLQFTLPPAMNIGTMTQLYNVAQDECSVLMLWTYLVAILALTVWSTIF 396

Query: 443 FKVVI 446
             +++
Sbjct: 430 LHLLV 396

BLAST of Tan0012821 vs. TAIR 10
Match: AT2G17500.3 (Auxin efflux carrier family protein )

HSP 1 Score: 177.2 bits (448), Expect = 3.0e-44
Identity = 126/425 (29.65%), Postives = 226/425 (53.18%), Query Frame = 0

Query: 23  AIMPLMKLLSLTAIGLLLSHPKIQMIPRATLRLLSKLVFALFLPCLIFTYLGQSITIENI 82
           A MP++++L ++ +G  ++  + ++ P      ++K+VF LF P L+F  L Q++T+E+I
Sbjct: 10  ASMPVIQVLFMSLVGAFMASDRCKLFPVEARNSMNKVVFVLFAPALMFANLAQTVTLEDI 69

Query: 83  AKWWFIPVNVLISTGIGCLLGFLVVILCRPPPPLVRFTIISTAFGNTGNLPLAIVSSVCH 142
             WWF+PVN+ ++  IG LLG+LVV + +PPP L    + + + GN GNLP+ +V ++C 
Sbjct: 70  ISWWFMPVNMGLTFLIGGLLGWLVVKILKPPPYLEGLIVATCSAGNMGNLPIILVPAICD 129

Query: 143 TADNPFGQH--CHSRGVSYVSFCQWVSVILALEFYEIVEEGTEIEELVETNDTGNDVSKP 202
              +PFG    C + G+SY SF       +AL  + I    T    L++           
Sbjct: 130 EDKSPFGNRSVCRTVGLSYASFS------MALGGFYI---WTYTFRLIK--------GSA 189

Query: 203 LLIEAEWPGIEEKETEHCKAPFIARIFNSISNVSQSTLPDFDHNSRENSSPMYPESIRCL 262
           + ++A    IEE E               I+  S ++  + DH +    +P   E+ + +
Sbjct: 190 MKVQA----IEESE--------------KIAIKSSNSDLEADHKTHLLGAPEDKEN-KVV 249

Query: 263 AEPRVVRKIRIVAEQTPIQHILQPPTIASLLAIIVGLVPQIKASFFGADAPLSFISDSLE 322
            E     +  +      ++ +L PPT+ +++  I G V  ++    G DAPL  +  + +
Sbjct: 250 KEKTGFWRKGVDFLHEILEELLAPPTLGAIIGFIFGAVRWLRNLIIGDDAPLRIVQSTAK 309

Query: 323 ILAGAMVPFVMLILGGMLAEGPNESSTLGLRTTIGISIARLLVLPVLGIGIVVAADKLNF 382
           +L    +P + +ILGG L +G   SS +     +GI   R + +P++GIGIV+ A  L F
Sbjct: 310 LLGDGTIPCMTIILGGNLIQG-LRSSAVKPMVVLGIVCVRYIAMPIIGIGIVLTAANLGF 369

Query: 383 LVHGDPMYKFVLLLQYTTPTAILLGAIASLRGYAVKEASALLFWEHVFALLSLTLYIFVY 442
           L   DP++++VL+LQ+T P A+ +G +  L   A  E S L+ W ++ A+L+LT++  ++
Sbjct: 370 L-PADPLFQYVLMLQFTLPPAMNIGTMTQLYNVAQDECSVLMLWTYLVAILALTVWSTIF 396

Query: 443 FKVVI 446
             +++
Sbjct: 430 LHLLV 396

BLAST of Tan0012821 vs. TAIR 10
Match: AT2G17500.2 (Auxin efflux carrier family protein )

HSP 1 Score: 177.2 bits (448), Expect = 3.0e-44
Identity = 126/425 (29.65%), Postives = 226/425 (53.18%), Query Frame = 0

Query: 23  AIMPLMKLLSLTAIGLLLSHPKIQMIPRATLRLLSKLVFALFLPCLIFTYLGQSITIENI 82
           A MP++++L ++ +G  ++  + ++ P      ++K+VF LF P L+F  L Q++T+E+I
Sbjct: 10  ASMPVIQVLFMSLVGAFMASDRCKLFPVEARNSMNKVVFVLFAPALMFANLAQTVTLEDI 69

Query: 83  AKWWFIPVNVLISTGIGCLLGFLVVILCRPPPPLVRFTIISTAFGNTGNLPLAIVSSVCH 142
             WWF+PVN+ ++  IG LLG+LVV + +PPP L    + + + GN GNLP+ +V ++C 
Sbjct: 70  ISWWFMPVNMGLTFLIGGLLGWLVVKILKPPPYLEGLIVATCSAGNMGNLPIILVPAICD 129

Query: 143 TADNPFGQH--CHSRGVSYVSFCQWVSVILALEFYEIVEEGTEIEELVETNDTGNDVSKP 202
              +PFG    C + G+SY SF       +AL  + I    T    L++           
Sbjct: 130 EDKSPFGNRSVCRTVGLSYASFS------MALGGFYI---WTYTFRLIK--------GSA 189

Query: 203 LLIEAEWPGIEEKETEHCKAPFIARIFNSISNVSQSTLPDFDHNSRENSSPMYPESIRCL 262
           + ++A    IEE E               I+  S ++  + DH +    +P   E+ + +
Sbjct: 190 MKVQA----IEESE--------------KIAIKSSNSDLEADHKTHLLGAPEDKEN-KVV 249

Query: 263 AEPRVVRKIRIVAEQTPIQHILQPPTIASLLAIIVGLVPQIKASFFGADAPLSFISDSLE 322
            E     +  +      ++ +L PPT+ +++  I G V  ++    G DAPL  +  + +
Sbjct: 250 KEKTGFWRKGVDFLHEILEELLAPPTLGAIIGFIFGAVRWLRNLIIGDDAPLRIVQSTAK 309

Query: 323 ILAGAMVPFVMLILGGMLAEGPNESSTLGLRTTIGISIARLLVLPVLGIGIVVAADKLNF 382
           +L    +P + +ILGG L +G   SS +     +GI   R + +P++GIGIV+ A  L F
Sbjct: 310 LLGDGTIPCMTIILGGNLIQG-LRSSAVKPMVVLGIVCVRYIAMPIIGIGIVLTAANLGF 369

Query: 383 LVHGDPMYKFVLLLQYTTPTAILLGAIASLRGYAVKEASALLFWEHVFALLSLTLYIFVY 442
           L   DP++++VL+LQ+T P A+ +G +  L   A  E S L+ W ++ A+L+LT++  ++
Sbjct: 370 L-PADPLFQYVLMLQFTLPPAMNIGTMTQLYNVAQDECSVLMLWTYLVAILALTVWSTIF 396

Query: 443 FKVVI 446
             +++
Sbjct: 430 LHLLV 396

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C9991.8e-16667.18Protein PIN-LIKES 2 OS=Arabidopsis thaliana OX=3702 GN=PILS2 PE=2 SV=1[more]
Q9LZN29.0e-7036.76Protein PIN-LIKES 6 OS=Arabidopsis thaliana OX=3702 GN=PILS6 PE=2 SV=1[more]
Q9SHL84.2e-4329.65Protein PIN-LIKES 5 OS=Arabidopsis thaliana OX=3702 GN=PILS5 PE=2 SV=1[more]
F4HWB61.2e-4229.47Protein PIN-LIKES 1 OS=Arabidopsis thaliana OX=3702 GN=PILS1 PE=2 SV=1[more]
Q9C9K52.7e-4229.71Protein PIN-LIKES 3 OS=Arabidopsis thaliana OX=3702 GN=PILS3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_038875937.11.3e-21990.37protein PIN-LIKES 2 [Benincasa hispida][more]
XP_023521529.19.4e-21887.94protein PIN-LIKES 2-like [Cucurbita pepo subsp. pepo] >XP_023521530.1 protein PI... [more]
XP_022939090.12.0e-21587.72protein PIN-LIKES 2 [Cucurbita moschata] >XP_022939092.1 protein PIN-LIKES 2 [Cu... [more]
KAG6579244.12.0e-21587.72Protein PIN-LIKES 2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022993212.14.4e-21587.50protein PIN-LIKES 2 [Cucurbita maxima] >XP_022993213.1 protein PIN-LIKES 2 [Cucu... [more]
Match NameE-valueIdentityDescription
A0A6J1FFU89.5e-21687.72protein PIN-LIKES 2 OS=Cucurbita moschata OX=3662 GN=LOC111445095 PE=4 SV=1[more]
A0A6J1JS572.1e-21587.50protein PIN-LIKES 2 OS=Cucurbita maxima OX=3661 GN=LOC111489301 PE=4 SV=1[more]
A0A6J1DDP01.4e-21185.96protein PIN-LIKES 2-like OS=Momordica charantia OX=3673 GN=LOC111020086 PE=4 SV=... [more]
A0A0A0KRM31.3e-20986.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G571470 PE=4 SV=1[more]
A0A5D3E4I52.5e-20885.59Protein PIN-LIKES 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold426G... [more]
Match NameE-valueIdentityDescription
AT1G71090.11.2e-16767.18Auxin efflux carrier family protein [more]
AT5G01990.16.4e-7136.76Auxin efflux carrier family protein [more]
AT2G17500.13.0e-4429.65Auxin efflux carrier family protein [more]
AT2G17500.33.0e-4429.65Auxin efflux carrier family protein [more]
AT2G17500.23.0e-4429.65Auxin efflux carrier family protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004776Membrane transport proteinPFAMPF03547Mem_transcoord: 24..437
e-value: 8.9E-80
score: 267.9
NoneNo IPR availablePANTHERPTHR31419:SF12AUXIN EFFLUX CARRIER FAMILY PROTEIN-RELATEDcoord: 10..444
IPR039305Protein PIN-LIKES 2/6PANTHERPTHR31419PROTEIN PIN-LIKES 2coord: 10..444

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0012821.1Tan0012821.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010315 auxin efflux
biological_process GO:0010252 auxin homeostasis
biological_process GO:0080162 intracellular auxin transport
biological_process GO:0010311 lateral root formation
biological_process GO:0040009 regulation of growth rate
biological_process GO:0055085 transmembrane transport
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0010329 auxin efflux transmembrane transporter activity