Tan0011800 (gene) Snake gourd v1

Overview
NameTan0011800
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionRibonuclease H-like domain containing protein
LocationLG01: 34740168 .. 34740768 (-)
RNA-Seq ExpressionTan0011800
SyntenyTan0011800
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAGACGTGCAGTGCAAATATGGGAGTCAGTATGACCAAAGGTAATAAATTTGTTGAAATTGAAATTACTTGTGCAAGACTTCTTACGGCTTGCTTTCTAAGACTTTGAAAGTGCTGAAGTAGAAATTTTGTGTGTAAGCTGTTGGGCAATCTGGTCTGATCGAAATGAAATATCCCAAGGCAAAATTACACCAAGTTTAATCAGACGTGTTGAATGGATTCAGGAGTACATTCAAGAAATTGGTAAAACTAGTGAGACAGGAAGATCGAAAGTAATAACAAAAACCTTAGCTGTTCAGAAGGAGAAATGGAAGAAGCCACCTGAAGGAATTCTGAAGTTGAATGTTGATGTTGTCTGCCATCCATCTCTACCGATCACGGGATTAGGAGCGATAATCAGGGATTCAAACGGACACATGCTGGTAGCTAGGTTGAAATTCTGTGAAGGACGTTTAGATCCTCTCTCAGCAGAGGCATTGGTAATGTTAAGTGGTATGAAAATGGCTGCTCAGAATGGTTTTACGAATCTATGGATTTCGTCAAACGCGCAGGTGCTGGTTAATGTTATTTACAAAAATGGCTTTCGATCGGCTACTTAA

mRNA sequence

ATGCAGACGTGCAGTGCAAATATGGGAGTCAGTATGACCAAAGACTTTGAAAGTGCTGAAGTAGAAATTTTGTGTGTAAGCTGTTGGGCAATCTGGTCTGATCGAAATGAAATATCCCAAGGCAAAATTACACCAAGTTTAATCAGACGTGTTGAATGGATTCAGGAGTACATTCAAGAAATTGGTAAAACTAGTGAGACAGGAAGATCGAAAGTAATAACAAAAACCTTAGCTGTTCAGAAGGAGAAATGGAAGAAGCCACCTGAAGGAATTCTGAAGTTGAATGTTGATGTTGTCTGCCATCCATCTCTACCGATCACGGGATTAGGAGCGATAATCAGGGATTCAAACGGACACATGCTGGTAGCTAGGTTGAAATTCTGTGAAGGACGTTTAGATCCTCTCTCAGCAGAGGCATTGGTAATGTTAAGTGGTATGAAAATGGCTGCTCAGAATGGTTTTACGAATCTATGGATTTCGTCAAACGCGCAGGTGCTGGTTAATGTTATTTACAAAAATGGCTTTCGATCGGCTACTTAA

Coding sequence (CDS)

ATGCAGACGTGCAGTGCAAATATGGGAGTCAGTATGACCAAAGACTTTGAAAGTGCTGAAGTAGAAATTTTGTGTGTAAGCTGTTGGGCAATCTGGTCTGATCGAAATGAAATATCCCAAGGCAAAATTACACCAAGTTTAATCAGACGTGTTGAATGGATTCAGGAGTACATTCAAGAAATTGGTAAAACTAGTGAGACAGGAAGATCGAAAGTAATAACAAAAACCTTAGCTGTTCAGAAGGAGAAATGGAAGAAGCCACCTGAAGGAATTCTGAAGTTGAATGTTGATGTTGTCTGCCATCCATCTCTACCGATCACGGGATTAGGAGCGATAATCAGGGATTCAAACGGACACATGCTGGTAGCTAGGTTGAAATTCTGTGAAGGACGTTTAGATCCTCTCTCAGCAGAGGCATTGGTAATGTTAAGTGGTATGAAAATGGCTGCTCAGAATGGTTTTACGAATCTATGGATTTCGTCAAACGCGCAGGTGCTGGTTAATGTTATTTACAAAAATGGCTTTCGATCGGCTACTTAA

Protein sequence

MQTCSANMGVSMTKDFESAEVEILCVSCWAIWSDRNEISQGKITPSLIRRVEWIQEYIQEIGKTSETGRSKVITKTLAVQKEKWKKPPEGILKLNVDVVCHPSLPITGLGAIIRDSNGHMLVARLKFCEGRLDPLSAEALVMLSGMKMAAQNGFTNLWISSNAQVLVNVIYKNGFRSAT
Homology
BLAST of Tan0011800 vs. NCBI nr
Match: XP_022131661.1 (uncharacterized protein LOC111004786 [Momordica charantia])

HSP 1 Score: 88.2 bits (217), Expect = 7.8e-14
Identity = 47/149 (31.54%), Postives = 72/149 (48.32%), Query Frame = 0

Query: 22  EILCVSCWAIWSDRNEISQGKITPSLIRRVEWIQEYIQEIGKTSETGRSKVITKTLAVQK 81
           ++   + WAIW DRN  + G    +   R  WI  Y Q   +  E  R      ++    
Sbjct: 53  DLAAFTXWAIWCDRNSXAHGSSVSTPALRCNWISSYFQNYSQAQENKRISPQQSSVPPPS 112

Query: 82  EKWKKPPEGILKLNVDVVCHPSLPITGLGAIIRDSNGHMLVARLKFCEGRLDPLSAEALV 141
            +W  P +  +K+N D  C  +   TGLG IIRD  G +LVA+  F    L+PL AE   
Sbjct: 113 CRWLPPRDSAMKVNSDAACRST--STGLGLIIRDHFGVLLVAKSMFLPMPLNPLFAEIRG 172

Query: 142 MLSGMKMAAQNGFTNLWISSNAQVLVNVI 171
           +L  +K+AA   +T L + S+ Q  + ++
Sbjct: 173 ILEALKLAASRSYTRLVVESDCQEAIRLV 199

BLAST of Tan0011800 vs. NCBI nr
Match: XP_024043083.1 (uncharacterized protein LOC112099827 [Citrus clementina])

HSP 1 Score: 87.8 bits (216), Expect = 1.0e-13
Identity = 53/136 (38.97%), Postives = 76/136 (55.88%), Query Frame = 0

Query: 19  AEVEILCVSCWAIWSDRNE-ISQGKITPS--LIRRVEWIQEYIQEIGKTSETGRSKVITK 78
           AE E++ V CW IWS RN+ I +GK + S  L  + E + +  Q + K         +TK
Sbjct: 708 AEAELMVVYCWVIWSARNKFIFEGKKSNSRILAAKAESVLKAYQRVSKPGTIH----VTK 767

Query: 79  TLAVQKEKWKKPPEGILKLNVDVVCHPSLPITGLGAIIRDSNGHMLVARLKFCEGRLDPL 138
              V ++KWK PP+ +LKLNVD   +     TGLGAIIRD+ G +L   +K  + R    
Sbjct: 768 NRGVDQQKWKPPPKNVLKLNVDAAVNSKDQKTGLGAIIRDAEGKILAVGIKQAQFRERVS 827

Query: 139 SAEALVMLSGMKMAAQ 152
            AEA  +L G+++A Q
Sbjct: 828 LAEAEAILWGLQVAKQ 839

BLAST of Tan0011800 vs. NCBI nr
Match: XP_023897447.1 (uncharacterized protein LOC112009345 [Quercus suber])

HSP 1 Score: 87.0 bits (214), Expect = 1.7e-13
Identity = 47/169 (27.81%), Postives = 81/169 (47.93%), Query Frame = 0

Query: 14   KDFESAEVEILCVSCWAIWSDRNEISQGKITPSLIRRVEWIQEYIQEIGKTSETGRSKVI 73
            K F+  ++ ++    WA W +RNEI  G    S    V+W+  Y+ E    +E+      
Sbjct: 915  KSFDEEKIILVVTVAWAFWCNRNEIRHGAEKKSPEAIVQWVNRYLLEYSAATES------ 974

Query: 74   TKTLAVQKE---KWKKPPEGILKLNVDVVCHPSLPITGLGAIIRDSNGHMLVARLKFCEG 133
                AV++E    W  PP  ILK+NVD     +L   G+GA++RD  G ++ A  +    
Sbjct: 975  --VPAVREEVSVTWNPPPPSILKVNVDGATTKNLNFVGVGAVVRDEQGRVVAAMSRKIPA 1034

Query: 134  RLDPLSAEALVMLSGMKMAAQNGFTNLWISSNAQVLVNVIYKNGFRSAT 180
             L PL  E     +G+++A   G+ N+ +  ++ ++V  +      S+T
Sbjct: 1035 PLGPLEVEVKAFEAGLQLAKDMGYQNIILEGDSLIIVRALCGISLSSST 1075

BLAST of Tan0011800 vs. NCBI nr
Match: TXG66206.1 (hypothetical protein EZV62_007481 [Acer yangbiense])

HSP 1 Score: 85.5 bits (210), Expect = 5.1e-13
Identity = 46/154 (29.87%), Postives = 77/154 (50.00%), Query Frame = 0

Query: 17  ESAEVEILCVSCWAIWSDRNEISQGKITPSLIRRVEWIQEYIQEIGKTSETGRSKVITKT 76
           +S   E+LCV CW +W  RN+   G +   + +  EW   ++ +     E  RSK+  +T
Sbjct: 111 KSPYFELLCVLCWCVWHRRNQSIHGSLAFPVSKIFEWGLAFLHDF---CEASRSKLKGQT 170

Query: 77  LAVQKEKWKKPPEGILKLNVDVVCHPSLPITGLGAIIRDSNGHMLVARLKFCEGRLDPLS 136
            +     W+    G  K+N D   H S  ++G+G +I+D++ H+  +  +       P  
Sbjct: 171 GSNVVPCWQALQLGAFKINTDAALHSSDKVSGIGVVIQDNDAHVRASLCQNLSAYFQPQI 230

Query: 137 AEALVMLSGMKMAAQNGFTNLWISSNAQVLVNVI 171
           AEAL +L G+ +A  NGF    + S+A  +VN I
Sbjct: 231 AEALAILKGLFLALNNGFVPAVLESDALTVVNSI 261

BLAST of Tan0011800 vs. NCBI nr
Match: TXG69190.1 (hypothetical protein EZV62_004125 [Acer yangbiense])

HSP 1 Score: 84.0 bits (206), Expect = 1.5e-12
Identity = 48/153 (31.37%), Postives = 80/153 (52.29%), Query Frame = 0

Query: 22  EILCVSCWAIWSDRNEISQGKITPSL--IRRVEWIQEYIQEI--GKTSETGRSKVITKTL 81
           E LCV  W +W  RN++   K + ++     ++W   +IQ+    KT +TG   V+ + +
Sbjct: 641 EFLCVVWWRVWYRRNQLVYEKSSQTVHDFDVLDWAASFIQDFKAAKTVDTG--SVVKQRV 700

Query: 82  AVQKEKWKKPPEGILKLNVDVVCHPSLPITGLGAIIRDSNGHMLVARLKFCEGRLDPLSA 141
           A    KWK  P G  K+N D        +TG+G +IRD  GH++ +  +   G L P + 
Sbjct: 701 A---PKWKPSPSGSYKINTDATLDCRAKVTGIGVVIRDCYGHVMASLCQSFPGLLQPQTV 760

Query: 142 EALVMLSGMKMAAQNGFTNLWISSNAQVLVNVI 171
           EA+ +L G ++A + G     I S++  +VN+I
Sbjct: 761 EAVAVLRGFRLALEAGLCPASIESDSLSVVNLI 788

BLAST of Tan0011800 vs. ExPASy TrEMBL
Match: A0A6J1BQ49 (uncharacterized protein LOC111004786 OS=Momordica charantia OX=3673 GN=LOC111004786 PE=4 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 3.8e-14
Identity = 47/149 (31.54%), Postives = 72/149 (48.32%), Query Frame = 0

Query: 22  EILCVSCWAIWSDRNEISQGKITPSLIRRVEWIQEYIQEIGKTSETGRSKVITKTLAVQK 81
           ++   + WAIW DRN  + G    +   R  WI  Y Q   +  E  R      ++    
Sbjct: 53  DLAAFTXWAIWCDRNSXAHGSSVSTPALRCNWISSYFQNYSQAQENKRISPQQSSVPPPS 112

Query: 82  EKWKKPPEGILKLNVDVVCHPSLPITGLGAIIRDSNGHMLVARLKFCEGRLDPLSAEALV 141
            +W  P +  +K+N D  C  +   TGLG IIRD  G +LVA+  F    L+PL AE   
Sbjct: 113 CRWLPPRDSAMKVNSDAACRST--STGLGLIIRDHFGVLLVAKSMFLPMPLNPLFAEIRG 172

Query: 142 MLSGMKMAAQNGFTNLWISSNAQVLVNVI 171
           +L  +K+AA   +T L + S+ Q  + ++
Sbjct: 173 ILEALKLAASRSYTRLVVESDCQEAIRLV 199

BLAST of Tan0011800 vs. ExPASy TrEMBL
Match: A0A5C7ICQ3 (RNase H domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_007481 PE=4 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 2.5e-13
Identity = 46/154 (29.87%), Postives = 77/154 (50.00%), Query Frame = 0

Query: 17  ESAEVEILCVSCWAIWSDRNEISQGKITPSLIRRVEWIQEYIQEIGKTSETGRSKVITKT 76
           +S   E+LCV CW +W  RN+   G +   + +  EW   ++ +     E  RSK+  +T
Sbjct: 111 KSPYFELLCVLCWCVWHRRNQSIHGSLAFPVSKIFEWGLAFLHDF---CEASRSKLKGQT 170

Query: 77  LAVQKEKWKKPPEGILKLNVDVVCHPSLPITGLGAIIRDSNGHMLVARLKFCEGRLDPLS 136
            +     W+    G  K+N D   H S  ++G+G +I+D++ H+  +  +       P  
Sbjct: 171 GSNVVPCWQALQLGAFKINTDAALHSSDKVSGIGVVIQDNDAHVRASLCQNLSAYFQPQI 230

Query: 137 AEALVMLSGMKMAAQNGFTNLWISSNAQVLVNVI 171
           AEAL +L G+ +A  NGF    + S+A  +VN I
Sbjct: 231 AEALAILKGLFLALNNGFVPAVLESDALTVVNSI 261

BLAST of Tan0011800 vs. ExPASy TrEMBL
Match: A0A5C7IIT4 (Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_004125 PE=4 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 7.2e-13
Identity = 48/153 (31.37%), Postives = 80/153 (52.29%), Query Frame = 0

Query: 22  EILCVSCWAIWSDRNEISQGKITPSL--IRRVEWIQEYIQEI--GKTSETGRSKVITKTL 81
           E LCV  W +W  RN++   K + ++     ++W   +IQ+    KT +TG   V+ + +
Sbjct: 641 EFLCVVWWRVWYRRNQLVYEKSSQTVHDFDVLDWAASFIQDFKAAKTVDTG--SVVKQRV 700

Query: 82  AVQKEKWKKPPEGILKLNVDVVCHPSLPITGLGAIIRDSNGHMLVARLKFCEGRLDPLSA 141
           A    KWK  P G  K+N D        +TG+G +IRD  GH++ +  +   G L P + 
Sbjct: 701 A---PKWKPSPSGSYKINTDATLDCRAKVTGIGVVIRDCYGHVMASLCQSFPGLLQPQTV 760

Query: 142 EALVMLSGMKMAAQNGFTNLWISSNAQVLVNVI 171
           EA+ +L G ++A + G     I S++  +VN+I
Sbjct: 761 EAVAVLRGFRLALEAGLCPASIESDSLSVVNLI 788

BLAST of Tan0011800 vs. ExPASy TrEMBL
Match: A0A803QGD0 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 4.6e-12
Identity = 45/155 (29.03%), Postives = 71/155 (45.81%), Query Frame = 0

Query: 16  FESAEVEILCVSCWAIWSDRNEISQGKITPSLIRRVEWIQEYIQEIGKTSETGRSKVITK 75
           +   ++E +    W IWSDRN    GK+  + I+ +     Y+Q+    +   +    ++
Sbjct: 114 YNKPDLESILCLMWFIWSDRNSYVHGKMVKTPIQMLTQSAAYLQQFQSVNSAAKPAASSR 173

Query: 76  TLAVQKEKWKKPPEGILKLNVDVVCHPSLPITGLGAIIRDSNGHMLVARLKFCEGRLDPL 135
           T      KW+ PPE   KLNVD     S    G+GAIIR+S G ++ A  K   G     
Sbjct: 174 TSPTPVTKWQPPPENKFKLNVDATLDSSRSKIGIGAIIRNSAGQVVGAMSKPAVGNFKSQ 233

Query: 136 SAEALVMLSGMKMAAQNGFTNLWISSNAQVLVNVI 171
             EA  M  G+  A Q      ++ ++  +LVN +
Sbjct: 234 EMEAKAMFVGLSWAKQYQIPIDYVETDCLILVNAL 268

BLAST of Tan0011800 vs. ExPASy TrEMBL
Match: A0A2P5EX40 (Ribonuclease H-like domain containing protein OS=Trema orientale OX=63057 GN=TorRG33x02_141250 PE=4 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 1.0e-11
Identity = 54/176 (30.68%), Postives = 85/176 (48.30%), Query Frame = 0

Query: 7   NMGVSMTKDFESAEVEILCVSCWAIWSDRNEISQG---KITPSLIRRV-EWIQEYIQEIG 66
           N  +S+       + E+  V  W IW DRN I  G   ++  SL+     W++E+ + +G
Sbjct: 11  NFLMSLKLKLSKQDFELWSVISWLIWRDRNSIFHGGVARLAESLLEDAGYWLREFQRLVG 70

Query: 67  KTSETGRSKVITKTLAVQKEKWKKPPEGILKLNVDVVCHPSLPITGLGAIIRDSNGHML- 126
                    + ++T+    +KWK P  G LKLNVD     S    G+G I+RD NG +L 
Sbjct: 71  -----SEKILASRTMINCDKKWKAPTMGQLKLNVDAAVKCSSGFIGIGVIVRDCNGMVLG 130

Query: 127 VARLKFCEGRLDPLSAEALVMLSGMKMAAQNGFTNLWISSNAQVLVNVIYKNGFRS 178
            + LKF  G L P  AE + +  G+K    +G     I ++AQ +V  + +  F +
Sbjct: 131 ASSLKFA-GHLSPFLAECVAVREGIKFKINHGIFPGVIEADAQNIVLALQEKTFNA 180

BLAST of Tan0011800 vs. TAIR 10
Match: AT4G09775.1 (BEST Arabidopsis thaliana protein match is: Ribonuclease H-like superfamily protein (TAIR:AT2G02650.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 55.8 bits (133), Expect = 4.0e-08
Identity = 38/138 (27.54%), Postives = 59/138 (42.75%), Query Frame = 0

Query: 29  WAIWSDRNEISQGKIT--PSLIRR------VEWIQEYIQEIGKTSETGRSKVITKTLAVQ 88
           W +W  RNE    +I   P  + +       EW++  I +   +  T +        + +
Sbjct: 2   WRLWKSRNEFLFQQIDRFPWKVAQKGEQEATEWVETTINDTANSHSTEQP---NDRPSGR 61

Query: 89  KEKWKKPPEGILKLNVDVVCHPSLPITGLGAIIRDSNGHMLVARLKFCEGRLDPLSAEAL 148
            ++W  PPEG LK N D         T    IIRDSNGH++ +     +     L AEAL
Sbjct: 62  SKEWSPPPEGYLKCNFDSGYVQGRDYTSTCWIIRDSNGHVIHSGCAKLQQSYSALQAEAL 121

Query: 149 VMLSGMKMAAQNGFTNLW 159
             L  ++M    G+  +W
Sbjct: 122 GFLHALQMVWIRGYRYVW 136

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
XP_022131661.17.8e-1431.54uncharacterized protein LOC111004786 [Momordica charantia][more]
XP_024043083.11.0e-1338.97uncharacterized protein LOC112099827 [Citrus clementina][more]
XP_023897447.11.7e-1327.81uncharacterized protein LOC112009345 [Quercus suber][more]
TXG66206.15.1e-1329.87hypothetical protein EZV62_007481 [Acer yangbiense][more]
TXG69190.11.5e-1231.37hypothetical protein EZV62_004125 [Acer yangbiense][more]
Match NameE-valueIdentityDescription
A0A6J1BQ493.8e-1431.54uncharacterized protein LOC111004786 OS=Momordica charantia OX=3673 GN=LOC111004... [more]
A0A5C7ICQ32.5e-1329.87RNase H domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_007481 ... [more]
A0A5C7IIT47.2e-1331.37Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_004125 PE=4 SV=1[more]
A0A803QGD04.6e-1229.03Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A2P5EX401.0e-1130.68Ribonuclease H-like domain containing protein OS=Trema orientale OX=63057 GN=Tor... [more]
Match NameE-valueIdentityDescription
AT4G09775.14.0e-0827.54BEST Arabidopsis thaliana protein match is: Ribonuclease H-like superfamily prot... [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 95..172
e-value: 3.0E-11
score: 43.2
NoneNo IPR availablePANTHERPTHR47074BNAC02G40300D PROTEINcoord: 79..171
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 94..177
e-value: 8.37459E-13
score: 60.0204

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0011800.1Tan0011800.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity