Tan0009873 (gene) Snake gourd v1

Overview
NameTan0009873
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionbeta-1,3-galactosyltransferase GALT1-like
LocationLG04: 9631123 .. 9634930 (-)
RNA-Seq ExpressionTan0009873
SyntenyTan0009873
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGAAAAGTTATGGCCGGGTTCTGCTTGCATCCTTGTTTATGTTGGTGTTGATGGTTTATGTTATCTTGAGAAGTTCAACTCGGCAAGATTATTATGCAACGCCATTGTACACTTCCGTTGATCCTCTTCAGTGGCTCAATCCTGCAGTTCCACCTGCAGTTCAAAATCCTGAAATTGGACATCAAGTGATCTCTGCTGACTCTATAATATCCAGTCTCTTCACTCAGGGGAATTTTACTGATGGAGAGAATCGAGCTCTTCAGACTTGGAACCATTTAAGAAGCATAATTAATTGTACTCGAGGTTTGCCTAATGCAGTAGAGGCCATTAAGGAAGCTGGAGGTGTATGGGATGTTCTCAAGACTTCGATTGAAAAGGAAAGGCTTGGTTATACTAATGAAAGTGCACGTGCGAAAGAGAAGCAATGCCCTCATTTTCTAACCAAAATGAATGCAACAAAGCTTGACAACAATGGTCATAAGCTGAGAATGCCTTGTGGCCTTACTCAGGGTTCTTCCATCACAGTCATTGGCATTCCAGATGGTCTTCTTGGAAATTTTCGAATTGACTTGACAGGGGAACCACTGCCTGGGGAGCCTGATCCACCCCTCATTTTGCATTATAATGTTAGGCTTTTGGGAGATAAGTTAACTGAGGATCCTGTAATTGTCCAGAATACCTGGACAGTTTCTCGTGATTGGGGAGATGAGGAGCGATGCCCATCCCCTGGTTCTGACGAGAATGGAAAAGGTATAAACGTTTATCATATTTTCTTTGAATAATTCCATGCATGCACATCAATGTTGAATAACTATTTTAGTCAAATTTCTGTATTTAAAATATGGAGTCTCGATTGAAATATGTTGCTTCTATTTATAAACAATTCAAGTCTGCTTACATATGATCTTACCTTGAAACTTTGTTACAAAGCAGCCCGTGTGTGCGATATTATATTATGGCTTGCTGATGTGAAAATTATTACTGCATATGCTTGCATAATTTTCAACAACATTGACTCAAGCCACACGATGTTCACATAATTGGTTTATCAAACGGCTTGGGAAATTGGGAACATAGCATCCATTAACTTCATAATATTGAACTGAATACATATTTCGCCAATCAAGTATTATTTCCATTCACTTTGAACTGATGTTGATTTTATATACTAGTGGATGAATTGGAAAAATGCAACAAGATAGTGGGCAATGTCGAAACTCGCCTTTCTGAGTCGAACAAGAATTTCAACAAATCAAAATCTATGGTGCAAGGAGCTAAAACAAGACCGTACTTCCCCTTCAAACTTGGTCAACCATTTGCTGCTACACTTAGAGTGGGAGTAGATGGAATCCAAATGACAGTTGATGGAAAGCATGTTACTTCTTTTGCTTACCGTGAAGTAATCTCACCTATTCTTGTATTCATTGTCTTTACATTGATCTTTGAACAATCAATCTAATGCCTGTTTTGGCTTGCTTCTTATTCAGACTCTGGAGCCATGGCTGGTCAGTGAAGTTAAGATTTCAGGAGACTTGAAATTAATTTCTGTCCTGGCAAGTGGTTTGCCCACATCCGAGGATTCAGATCATATTGTTAACATAGAAGCATTAAAATCTTCTCCCCTCTCTCTGGAAAGACCATTAGATCTCTTCATTGGGGTTTTCTCCACAGCAAACAATTTTAAATATCGAATGGCCGTTCGAAGAACATGGATCCAGTATCCTGAAGTACGGTCAGGCTCTGTTGCAGTTCGCTTTTTTGTTGGGTTGGTGAGCTTCCTTCTCATGCACTCTTCTTTGATTGCAAAGAATCTCAATACAATTGGGCTATCCATACATATTGAACCTAGTGATTTCATAAGGCACTAAAAAGCTTAGCTGTTCGTCGCATGAATGAATAGTTTACTTCCAAAATATATCTTGAGGCCAATTCTAAGTCTGTTCATAATGCTTCCAAGTGAAATTGAATGAGGTGCTTGATACGATCAATCTGCCACACTATTTCAAAAATTTATTGGTTGGATTCTTGAATTGGAATGATTCTTCAGCTGTTCATATGATAGTATCAATCCAAGAAGAATATGATTTTTAGTGGCTTACACTCAGTTTGCGTTTGCAGCATAAGAACCAAATAGTTAATGAGGAATTGTGGGATGAGGCCCGAACATATGGTGACATACAGATGATGCCTTTTGTTGACTATTACAGCCTTATTACCTGGAAAACATTGGGTATCTGCATCTTCGGGGTAAAGATTCGATCTTTCACTAAACGATGTGAATTTATCATACAATTCATGTTAACAATCTCACCGCTTTGACAGGCAGAGATTGCTTCAGCAAAGTATATCATGAAGACAGATGATGATGCCTTTGTTCGAGTGGATGAAGTTCTAGCTTCTTTAAAAAGGATTGATGTACAAAGTGGATTGCTTTATGGACTCATCAACTCAGATTCTCAACCTCACAGAGACCCTGAGAGCAAGTGGTATATTAGTATGGAGGTAATTTTCCGCATTGGTTTTTGCTTATATGAGCTAAGGATCTAACATTGCGTGGATAACCCAAGGCTAAAGATACACTTCACTTTTTTTCACTTTTATGGGTCTTAACTATACTTTGCTTTACTAAAACCTCTGATTTGCAACAACAAAAGAACTGCCCTAAAAGAAACGTTAACATGCAACCATTATTTCCCCATGTTCGAGCTGGTATGCTATGAAGTATGAATTTAGCTGGTATTTCTGCTAAAAAAGTTTGTCGAATATTATTTACATTTTTTAACAAGCTTGCTCAGAATATGGTTCCACTTGCCTTTGTAGTGCATACTCAATTAATTTAATAAAAGCAAATGAATTTTTCTTTTAGTCCAACAATTTGTGAGATAAGGAGATTTTGACTCTCTAATCTCTCTAATCTCTTGGTCGAGGATATATTTTGTTAGGCCCCCGATCATTAAAAAGTATTTTAGAAGGGGCAAGAGAGTCATTGTCTCTAGTGAACAGGGGACCACAGAGAATTGAGGGATAGTACGTTGTGGGAGTGAGGGTGCTGTGTAAAAGGCTGTCGAGTCTGTTAGGGAGGGCATGTTTTCATTTTGGTAAACAAATGTTTTTGAGAGAAGGAGAGGACCAGCTTCTCGGATTGCTGGGAGTGTGTTTGTTTGGCTCTTGTTCTTCTTACCCTCTTAATGAAATAACCCCTCCTCAGTGTCGAAGAACTCTCGAACACGAAGGAGTTGGGTCCTCATTCCCCTATTTTTCGTATTATTATACTTGTTGTTATTGTTAGGCATAGAACTTCTATACAGGTACCTTACATATTTCTTAACTAATTAAGATAGGTTGATGTTTGGTAAATTAATATTTCTTTAATACCATCTTACCTTCATTGATTGCTCCAATGTAGGAATGGCCCGAAGATAATTATCCAACATGGGCACATGGTCCTGGGTATGTAGTGTCTAATGACATAGCAAAGACAATATCCAAAAAATACAAAGAGGGCCGCTTAAAGGTATGTCATTTCTTTGCTCGAAAAAGGTATTATTCCATAAATGAGAAACTAAAAACAGGATGTTGCATCTTCAGATGTTTAAATTAGAAGATGTAGCCATGGGAATCTGGATAGAAAACATGAAGAGAGAGGGCCTCAAAATTCGTTACGAGAAGGACGATAGAATTCATATTGAAGGTTGTAAGGATGACTATATGGTTGCTCACTACCAGGGACCTCGGGAGATGCTCTGTTTGTGGCAGAAACTTCAGGAAGGAAATGGTATTAGATGCTGTGGTTCTGATAACTAA

mRNA sequence

ATGAAGAAAAGTTATGGCCGGGTTCTGCTTGCATCCTTGTTTATGTTGGTGTTGATGGTTTATGTTATCTTGAGAAGTTCAACTCGGCAAGATTATTATGCAACGCCATTGTACACTTCCGTTGATCCTCTTCAGTGGCTCAATCCTGCAGTTCCACCTGCAGTTCAAAATCCTGAAATTGGACATCAAGTGATCTCTGCTGACTCTATAATATCCAGTCTCTTCACTCAGGGGAATTTTACTGATGGAGAGAATCGAGCTCTTCAGACTTGGAACCATTTAAGAAGCATAATTAATTGTACTCGAGGTTTGCCTAATGCAGTAGAGGCCATTAAGGAAGCTGGAGGTGTATGGGATGTTCTCAAGACTTCGATTGAAAAGGAAAGGCTTGGTTATACTAATGAAAGTGCACGTGCGAAAGAGAAGCAATGCCCTCATTTTCTAACCAAAATGAATGCAACAAAGCTTGACAACAATGGTCATAAGCTGAGAATGCCTTGTGGCCTTACTCAGGGTTCTTCCATCACAGTCATTGGCATTCCAGATGGTCTTCTTGGAAATTTTCGAATTGACTTGACAGGGGAACCACTGCCTGGGGAGCCTGATCCACCCCTCATTTTGCATTATAATGTTAGGCTTTTGGGAGATAAGTTAACTGAGGATCCTGTAATTGTCCAGAATACCTGGACAGTTTCTCGTGATTGGGGAGATGAGGAGCGATGCCCATCCCCTGGTTCTGACGAGAATGGAAAAGTGGATGAATTGGAAAAATGCAACAAGATAGTGGGCAATGTCGAAACTCGCCTTTCTGAGTCGAACAAGAATTTCAACAAATCAAAATCTATGGTGCAAGGAGCTAAAACAAGACCGTACTTCCCCTTCAAACTTGGTCAACCATTTGCTGCTACACTTAGAGTGGGAGTAGATGGAATCCAAATGACAGTTGATGGAAAGCATGTTACTTCTTTTGCTTACCGTGAAACTCTGGAGCCATGGCTGGTCAGTGAAGTTAAGATTTCAGGAGACTTGAAATTAATTTCTGTCCTGGCAAGTGGTTTGCCCACATCCGAGGATTCAGATCATATTGTTAACATAGAAGCATTAAAATCTTCTCCCCTCTCTCTGGAAAGACCATTAGATCTCTTCATTGGGGTTTTCTCCACAGCAAACAATTTTAAATATCGAATGGCCGTTCGAAGAACATGGATCCAGTATCCTGAAGTACGGTCAGGCTCTGTTGCAGTTCGCTTTTTTGTTGGGTTGCATAAGAACCAAATAGTTAATGAGGAATTGTGGGATGAGGCCCGAACATATGGTGACATACAGATGATGCCTTTTGTTGACTATTACAGCCTTATTACCTGGAAAACATTGGGTATCTGCATCTTCGGGGCAGAGATTGCTTCAGCAAAGTATATCATGAAGACAGATGATGATGCCTTTGTTCGAGTGGATGAAGTTCTAGCTTCTTTAAAAAGGATTGATGTACAAAGTGGATTGCTTTATGGACTCATCAACTCAGATTCTCAACCTCACAGAGACCCTGAGAGCAAGTGGTATATTAGTATGGAGGAATGGCCCGAAGATAATTATCCAACATGGGCACATGGTCCTGGGTATGTAGTGTCTAATGACATAGCAAAGACAATATCCAAAAAATACAAAGAGGGCCGCTTAAAGATGTTTAAATTAGAAGATGTAGCCATGGGAATCTGGATAGAAAACATGAAGAGAGAGGGCCTCAAAATTCGTTACGAGAAGGACGATAGAATTCATATTGAAGGTTGTAAGGATGACTATATGGTTGCTCACTACCAGGGACCTCGGGAGATGCTCTGTTTGTGGCAGAAACTTCAGGAAGGAAATGGTATTAGATGCTGTGGTTCTGATAACTAA

Coding sequence (CDS)

ATGAAGAAAAGTTATGGCCGGGTTCTGCTTGCATCCTTGTTTATGTTGGTGTTGATGGTTTATGTTATCTTGAGAAGTTCAACTCGGCAAGATTATTATGCAACGCCATTGTACACTTCCGTTGATCCTCTTCAGTGGCTCAATCCTGCAGTTCCACCTGCAGTTCAAAATCCTGAAATTGGACATCAAGTGATCTCTGCTGACTCTATAATATCCAGTCTCTTCACTCAGGGGAATTTTACTGATGGAGAGAATCGAGCTCTTCAGACTTGGAACCATTTAAGAAGCATAATTAATTGTACTCGAGGTTTGCCTAATGCAGTAGAGGCCATTAAGGAAGCTGGAGGTGTATGGGATGTTCTCAAGACTTCGATTGAAAAGGAAAGGCTTGGTTATACTAATGAAAGTGCACGTGCGAAAGAGAAGCAATGCCCTCATTTTCTAACCAAAATGAATGCAACAAAGCTTGACAACAATGGTCATAAGCTGAGAATGCCTTGTGGCCTTACTCAGGGTTCTTCCATCACAGTCATTGGCATTCCAGATGGTCTTCTTGGAAATTTTCGAATTGACTTGACAGGGGAACCACTGCCTGGGGAGCCTGATCCACCCCTCATTTTGCATTATAATGTTAGGCTTTTGGGAGATAAGTTAACTGAGGATCCTGTAATTGTCCAGAATACCTGGACAGTTTCTCGTGATTGGGGAGATGAGGAGCGATGCCCATCCCCTGGTTCTGACGAGAATGGAAAAGTGGATGAATTGGAAAAATGCAACAAGATAGTGGGCAATGTCGAAACTCGCCTTTCTGAGTCGAACAAGAATTTCAACAAATCAAAATCTATGGTGCAAGGAGCTAAAACAAGACCGTACTTCCCCTTCAAACTTGGTCAACCATTTGCTGCTACACTTAGAGTGGGAGTAGATGGAATCCAAATGACAGTTGATGGAAAGCATGTTACTTCTTTTGCTTACCGTGAAACTCTGGAGCCATGGCTGGTCAGTGAAGTTAAGATTTCAGGAGACTTGAAATTAATTTCTGTCCTGGCAAGTGGTTTGCCCACATCCGAGGATTCAGATCATATTGTTAACATAGAAGCATTAAAATCTTCTCCCCTCTCTCTGGAAAGACCATTAGATCTCTTCATTGGGGTTTTCTCCACAGCAAACAATTTTAAATATCGAATGGCCGTTCGAAGAACATGGATCCAGTATCCTGAAGTACGGTCAGGCTCTGTTGCAGTTCGCTTTTTTGTTGGGTTGCATAAGAACCAAATAGTTAATGAGGAATTGTGGGATGAGGCCCGAACATATGGTGACATACAGATGATGCCTTTTGTTGACTATTACAGCCTTATTACCTGGAAAACATTGGGTATCTGCATCTTCGGGGCAGAGATTGCTTCAGCAAAGTATATCATGAAGACAGATGATGATGCCTTTGTTCGAGTGGATGAAGTTCTAGCTTCTTTAAAAAGGATTGATGTACAAAGTGGATTGCTTTATGGACTCATCAACTCAGATTCTCAACCTCACAGAGACCCTGAGAGCAAGTGGTATATTAGTATGGAGGAATGGCCCGAAGATAATTATCCAACATGGGCACATGGTCCTGGGTATGTAGTGTCTAATGACATAGCAAAGACAATATCCAAAAAATACAAAGAGGGCCGCTTAAAGATGTTTAAATTAGAAGATGTAGCCATGGGAATCTGGATAGAAAACATGAAGAGAGAGGGCCTCAAAATTCGTTACGAGAAGGACGATAGAATTCATATTGAAGGTTGTAAGGATGACTATATGGTTGCTCACTACCAGGGACCTCGGGAGATGCTCTGTTTGTGGCAGAAACTTCAGGAAGGAAATGGTATTAGATGCTGTGGTTCTGATAACTAA

Protein sequence

MKKSYGRVLLASLFMLVLMVYVILRSSTRQDYYATPLYTSVDPLQWLNPAVPPAVQNPEIGHQVISADSIISSLFTQGNFTDGENRALQTWNHLRSIINCTRGLPNAVEAIKEAGGVWDVLKTSIEKERLGYTNESARAKEKQCPHFLTKMNATKLDNNGHKLRMPCGLTQGSSITVIGIPDGLLGNFRIDLTGEPLPGEPDPPLILHYNVRLLGDKLTEDPVIVQNTWTVSRDWGDEERCPSPGSDENGKVDELEKCNKIVGNVETRLSESNKNFNKSKSMVQGAKTRPYFPFKLGQPFAATLRVGVDGIQMTVDGKHVTSFAYRETLEPWLVSEVKISGDLKLISVLASGLPTSEDSDHIVNIEALKSSPLSLERPLDLFIGVFSTANNFKYRMAVRRTWIQYPEVRSGSVAVRFFVGLHKNQIVNEELWDEARTYGDIQMMPFVDYYSLITWKTLGICIFGAEIASAKYIMKTDDDAFVRVDEVLASLKRIDVQSGLLYGLINSDSQPHRDPESKWYISMEEWPEDNYPTWAHGPGYVVSNDIAKTISKKYKEGRLKMFKLEDVAMGIWIENMKREGLKIRYEKDDRIHIEGCKDDYMVAHYQGPREMLCLWQKLQEGNGIRCCGSDN
Homology
BLAST of Tan0009873 vs. ExPASy Swiss-Prot
Match: Q8L7F9 (Beta-1,3-galactosyltransferase GALT1 OS=Arabidopsis thaliana OX=3702 GN=GALT1 PE=1 SV=1)

HSP 1 Score: 783.9 bits (2023), Expect = 1.4e-225
Identity = 385/640 (60.16%), Postives = 480/640 (75.00%), Query Frame = 0

Query: 1   MKKSYGRVLLASLFM-LVLMVYVILRSSTRQDYYATPLYTSVD-----PLQWLNPAVPPA 60
           MK+ YG +L+ S+ M L +  YV L +   + Y        V      P++WL   +P  
Sbjct: 1   MKRFYGGLLVVSMCMFLTVYRYVDLNTPVEKPYITAAASVVVTPNTTLPMEWLRITLPDF 60

Query: 61  VQNPEIGHQVISAD--SIISSLFTQGNFTDGENRALQTWNHLRSIINCTRGLPNAVEAIK 120
           ++      + IS D  +++S LF + N +  E   L TWN L S+++  + L N V+AIK
Sbjct: 61  MKEARNTQEAISGDDIAVVSGLFVEQNVSKEEREPLLTWNRLESLVDNAQSLVNGVDAIK 120

Query: 121 EAGGVWDVLKTSIEKERLGYTNE--SARAKEKQCPHFLTKMNATKLDNNGHKLRMPCGLT 180
           EAG VW+ L +++E ++L   NE  + + KE+ CP FL+KMNAT+ D +  KL++PCGLT
Sbjct: 121 EAGIVWESLVSAVEAKKLVDVNENQTRKGKEELCPQFLSKMNATEADGSSLKLQIPCGLT 180

Query: 181 QGSSITVIGIPDGLLGNFRIDLTGEPLPGEPDPPLILHYNVRLLGDKLTEDPVIVQNTWT 240
           QGSSITVIGIPDGL+G+FRIDLTG+PLPGEPDPP+I+HYNVRLLGDK TEDPVIVQN+WT
Sbjct: 181 QGSSITVIGIPDGLVGSFRIDLTGQPLPGEPDPPIIVHYNVRLLGDKSTEDPVIVQNSWT 240

Query: 241 VSRDWGDEERCPSPGSDENGKVDELEKCNKIVGNVETRLSESNKNFNKSKSMV---QGAK 300
            S+DWG EERCP    D N KVD+L++CNK+VG    R S ++   N S+ +    + +K
Sbjct: 241 ASQDWGAEERCPKFDPDMNKKVDDLDECNKMVGGEINRTSSTSLQSNTSRGVPVAREASK 300

Query: 301 TRPYFPFKLGQPFAATLRVGVDGIQMTVDGKHVTSFAYRETLEPWLVSEVKISGDLKLIS 360
              YFPFK G    ATLRVG +G+QMTVDGKH+TSFA+R+TLEPWLVSE++I+GD +LIS
Sbjct: 301 HEKYFPFKQGFLSVATLRVGTEGMQMTVDGKHITSFAFRDTLEPWLVSEIRITGDFRLIS 360

Query: 361 VLASGLPTSEDSDHIVNIEALKSSPLSLERPLDLFIGVFSTANNFKYRMAVRRTWIQYPE 420
           +LASGLPTSE+S+H+V++EALKS  LS  RPLDL IGVFSTANNFK RMAVRRTW+QY +
Sbjct: 361 ILASGLPTSEESEHVVDLEALKSPTLSPLRPLDLVIGVFSTANNFKRRMAVRRTWMQYDD 420

Query: 421 VRSGSVAVRFFVGLHKNQIVNEELWDEARTYGDIQMMPFVDYYSLITWKTLGICIFGAEI 480
           VRSG VAVRFFVGLHK+ +VN ELW+EARTYGD+Q+MPFVDYYSLI+WKTL ICIFG E+
Sbjct: 421 VRSGRVAVRFFVGLHKSPLVNLELWNEARTYGDVQLMPFVDYYSLISWKTLAICIFGTEV 480

Query: 481 ASAKYIMKTDDDAFVRVDEVLASLKRIDVQSGLLYGLINSDSQPHRDPESKWYISMEEWP 540
            SAK+IMKTDDDAFVRVDEVL SL   +   GL+YGLINSDSQP R+P+SKWYIS EEWP
Sbjct: 481 DSAKFIMKTDDDAFVRVDEVLLSLSMTNNTRGLIYGLINSDSQPIRNPDSKWYISYEEWP 540

Query: 541 EDNYPTWAHGPGYVVSNDIAKTISKKYKEGRLKMFKLEDVAMGIWIENMKREGLKIRYEK 600
           E+ YP WAHGPGY+VS DIA+++ K +KEG LKMFKLEDVAMGIWI  + + GL+  YE 
Sbjct: 541 EEKYPPWAHGPGYIVSRDIAESVGKLFKEGNLKMFKLEDVAMGIWIAELTKHGLEPHYEN 600

Query: 601 DDRIHIEGCKDDYMVAHYQGPREMLCLWQKLQEGNGIRCC 628
           D RI  +GCKD Y+VAHYQ P EM CLW+K QE     CC
Sbjct: 601 DGRIISDGCKDGYVVAHYQSPAEMTCLWRKYQETKRSLCC 640

BLAST of Tan0009873 vs. ExPASy Swiss-Prot
Match: Q9ASW1 (Hydroxyproline O-galactosyltransferase GALT3 OS=Arabidopsis thaliana OX=3702 GN=GALT3 PE=2 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 3.5e-144
Identity = 263/557 (47.22%), Postives = 356/557 (63.91%), Query Frame = 0

Query: 74  LFTQGNFTDGE--NRALQTWNHLRSIINCTRGLPNAVEAIKEAGGVWDVLKTSIEKERLG 133
           LF+  +F   E  +  +  W+ +R  +     LP   + I+EA      L   I +E+  
Sbjct: 76  LFSNKSFFGEEEVSNGMLVWSRMRPFLERPDALPETAQGIEEATLAMKGLVLEINREKRA 135

Query: 134 YTNESARAKEKQ-CPHFLTKMNATKLDNNGHKLRMPCGLTQGSSITVIGIPDGLLGNFRI 193
           Y++     + ++ CP F+T  +      +   L +PCGL + SSIT++GIPD    +F+I
Sbjct: 136 YSSGMVSKEIRRICPDFVTAFDKDLSGLSHVLLELPCGLIEDSSITLVGIPDEHSSSFQI 195

Query: 194 DLTGEPLPGEPDPPLILHYNVRLLGDKLTEDPVIVQNTWTVSRDWGDEERCPSPGSDENG 253
            L G  L GE   P+IL YNV          P IVQNTWT    WG+EERC   GS +N 
Sbjct: 196 QLVGSGLSGETRRPIILRYNVNF------SKPSIVQNTWTEKLGWGNEERCQYHGSLKNH 255

Query: 254 KVDELEKCNKIVGNVETRLSESNKNFNKSKSMVQGAKTRPYFPFKLGQPFAATLRVGVDG 313
            VDEL  CNK  G +   +SE + N + +  +   + +   FPF  G PF A L  G++G
Sbjct: 256 LVDELPLCNKQTGRI---ISEKSSNDDATMEL---SLSNANFPFLKGSPFTAALWFGLEG 315

Query: 314 IQMTVDGKHVTSFAYRETLEPWLVSEVKISGDLKLISVLASGLPTSEDSDHIVNIEALKS 373
             MT++G+H TSFAYRE LEPWLVS VK+SG LK++SVLA+ LP  +D   ++  E LK+
Sbjct: 316 FHMTINGRHETSFAYREKLEPWLVSAVKVSGGLKILSVLATRLPIPDDHASLIIEEKLKA 375

Query: 374 SPLSLERPLDLFIGVFSTANNFKYRMAVRRTWIQYPEVRSGSVAVRFFVGLHKNQIVNEE 433
             LS  R ++L +GVFST NNFK RMA+RR+W+QY  VRSG VAVRF +GLH N+ VN E
Sbjct: 376 PSLSGTR-IELLVGVFSTGNNFKRRMALRRSWMQYEAVRSGKVAVRFLIGLHTNEKVNLE 435

Query: 434 LWDEARTYGDIQMMPFVDYYSLITWKTLGICIFGAEIASAKYIMKTDDDAFVRVDEVLAS 493
           +W E++ YGDIQ MPFVDYY L++ KT+ +CI G ++  AKYIMKTDDDAFVR+DE+L+S
Sbjct: 436 MWRESKAYGDIQFMPFVDYYGLLSLKTVALCILGTKVIPAKYIMKTDDDAFVRIDELLSS 495

Query: 494 LKRIDVQSGLLYGLINSDSQPHRDPESKWYISMEEWPEDNYPTWAHGPGYVVSNDIAKTI 553
           L+     S LLYGLI+ DS P R+  SKW+I  EEWP D+YP WAHGPGY++S+DIAK +
Sbjct: 496 LEE-RPSSALLYGLISFDSSPDREQGSKWFIPKEEWPLDSYPPWAHGPGYIISHDIAKFV 555

Query: 554 SKKYKEGRLKMFKLEDVAMGIWIENMKREGLKIRYEKDDRIHIEGCKDDYMVAHYQGPRE 613
            K +++  L +FKLEDVAMGIWI+   +   +++Y  D R H   CK +Y++ HYQ PR 
Sbjct: 556 VKGHRQRDLGLFKLEDVAMGIWIQQFNQTIKRVKYINDKRFHNSDCKSNYILVHYQTPRL 615

Query: 614 MLCLWQKLQEGNGIRCC 628
           +LCLW+KLQ+ N   CC
Sbjct: 616 ILCLWEKLQKENQSICC 618

BLAST of Tan0009873 vs. ExPASy Swiss-Prot
Match: Q8RX55 (Hydroxyproline O-galactosyltransferase GALT5 OS=Arabidopsis thaliana OX=3702 GN=GALT5 PE=1 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 4.3e-94
Identity = 199/542 (36.72%), Postives = 295/542 (54.43%), Query Frame = 0

Query: 107 AVEAIKEAGGVWDV---LKTSIEKERL-GYTNESARAKEKQCPHFLTKMNATKLDNNGHK 166
           +VE  K A   W +   L   +E  RL     +  + K   CPH ++   +  ++     
Sbjct: 133 SVELHKSAKEAWQLGRKLWKELESGRLEKLVEKPEKNKPDSCPHSVSLTGSEFMNRENKL 192

Query: 167 LRMPCGLTQGSSITVIGIP----------DGLLGNFRIDLTGEPLPGEPDPPLILHYNVR 226
           + +PCGLT GS IT++G P            L+  F I+L G       DPP ILH+N R
Sbjct: 193 MELPCGLTLGSHITLVGRPRKAHPKEGDWSKLVSQFVIELQGLKTVEGEDPPRILHFNPR 252

Query: 227 LLGDKLTEDPVIVQNTWTVSRDWGDEERCPS-PGSDENGKVDELEKCNKIVGNVETRLSE 286
           L GD  ++ PVI QN+      WG  +RC      D+   VD   KC K + + +   SE
Sbjct: 253 LKGD-WSKKPVIEQNS-CYRMQWGPAQRCEGWKSRDDEETVDSHVKCEKWIRD-DDNYSE 312

Query: 287 SNKN---FNKSKSMVQGAKTRPYFPFKLGQPFAATLRVGVDGIQMTVDGKHVTSFAYRET 346
            ++     N+     +  K    FPF   + F  TL  G++G  + VDGKHVTSF YR  
Sbjct: 313 GSRARWWLNRLIGRRKRVKVEWPFPFVEEKLFVLTLSAGLEGYHINVDGKHVTSFPYRTG 372

Query: 347 LEPWLVSEVKISGDLKLISVLASGLPTSEDS---DHIVNIEALKSSPLSLERPLDLFIGV 406
                 + + ++GD+ + SV  + LPTS  S      + +     +P+  + P+++FIG+
Sbjct: 373 FTLEDATGLTVNGDIDVHSVFVASLPTSHPSFAPQRHLELSKRWQAPVVPDGPVEIFIGI 432

Query: 407 FSTANNFKYRMAVRRTWIQYPEVRSGSVAVRFFVGLHKNQIVNEELWDEARTYGDIQMMP 466
            S  N+F  RMAVR++W+Q+  + S  V  RFFV LH  + VN EL  EA  +GDI ++P
Sbjct: 433 LSAGNHFSERMAVRKSWMQHVLITSAKVVARFFVALHGRKEVNVELKKEAEYFGDIVLVP 492

Query: 467 FVDYYSLITWKTLGICIFGAEIASAKYIMKTDDDAFVRVDEVLASLKRIDVQSGLLYGLI 526
           ++D Y L+  KT+ IC  GA   SAKYIMK DDD FV++  V+  +K++     L  G +
Sbjct: 493 YMDSYDLVVLKTVAICEHGALAFSAKYIMKCDDDTFVKLGAVINEVKKVPEGRSLYIGNM 552

Query: 527 NSDSQPHRDPESKWYISMEEWPEDNYPTWAHGPGYVVSNDIAKTISKKYKEGRLKMFKLE 586
           N   +P R    KW ++ EEWPE++YP +A+GPGYV+S+DIA+ I  K++  +L++FK+E
Sbjct: 553 NYYHKPLRG--GKWAVTYEEWPEEDYPPYANGPGYVLSSDIARFIVDKFERHKLRLFKME 612

Query: 587 DVAMGIWIENMKREGLKIRYEKDDRIHIEGCKDDYMVAHYQGPREMLCLWQKLQEGNGIR 628
           DV++G+W+E+ K     + Y    R    GC ++Y  AHYQ PR+M+CLW KL   N   
Sbjct: 613 DVSVGMWVEHFKNTTNPVDYRHSLRFCQFGCVENYYTAHYQSPRQMICLWDKLLRQNKPE 669

BLAST of Tan0009873 vs. ExPASy Swiss-Prot
Match: Q9LV16 (Hydroxyproline O-galactosyltransferase GALT6 OS=Arabidopsis thaliana OX=3702 GN=GALT6 PE=2 SV=2)

HSP 1 Score: 345.1 bits (884), Expect = 1.6e-93
Identity = 203/558 (36.38%), Postives = 302/558 (54.12%), Query Frame = 0

Query: 107 AVEAIKEAGGVWDVLKTSIEKERLGYTNESARAKEKQ--------CPHFLTKMNATKLDN 166
           +VE  K A   W+V +   E+   G T ++   ++K+               +  + L  
Sbjct: 125 SVELHKSAKVAWEVGRKIWEELESGKTLKALEKEKKKKIEEHGTNSCSLSVSLTGSDLLK 184

Query: 167 NGHKLRMPCGLTQGSSITVIGIPDGL-------------------LGNFRIDLTGEPLPG 226
            G+ + +PCGLT GS ITV+G P                      +  F+++L G     
Sbjct: 185 RGNIMELPCGLTLGSHITVVGKPRAAHSEKDPKISMLKEGDEAVKVSQFKLELQGLKAVE 244

Query: 227 EPDPPLILHYNVRLLGDKLTEDPVIVQNTWTVSRDWGDEERCPSPGS-DENGKVDELEKC 286
             +PP ILH N RL GD  +  PVI QNT      WG  +RC    S D+   VD   KC
Sbjct: 245 GEEPPRILHLNPRLKGD-WSGKPVIEQNT-CYRMQWGSAQRCEGWRSRDDEETVDGQVKC 304

Query: 287 NKIV--GNVETRLSESNKNFNKSKSMVQGAKTRPY----FPFKLGQPFAATLRVGVDGIQ 346
            K     ++ ++  ES+K  +   S + G   +      FPF + + F  TL  G++G  
Sbjct: 305 EKWARDDSITSKEEESSKAASWWLSRLIGRSKKVTVEWPFPFTVDKLFVLTLSAGLEGYH 364

Query: 347 MTVDGKHVTSFAYRETLEPWLVSEVKISGDLKLISVLASGLPTSEDS---DHIVNIEALK 406
           ++VDGKHVTSF YR        + + I+GD+ + SV A  LPTS  S      + + +  
Sbjct: 365 VSVDGKHVTSFPYRTGFTLEDATGLTINGDIDVHSVFAGSLPTSHPSFSPQRHLELSSNW 424

Query: 407 SSPLSLERPLDLFIGVFSTANNFKYRMAVRRTWIQYPEVRSGSVAVRFFVGLHKNQIVNE 466
            +P   +  +D+FIG+ S  N+F  RMAVRR+W+Q+  V+S  V  RFFV LH  + VN 
Sbjct: 425 QAPSLPDEQVDMFIGILSAGNHFAERMAVRRSWMQHKLVKSSKVVARFFVALHSRKEVNV 484

Query: 467 ELWDEARTYGDIQMMPFVDYYSLITWKTLGICIFGAEIASAKYIMKTDDDAFVRVDEVLA 526
           EL  EA  +GDI ++P++D Y L+  KT+ IC +GA   +AK+IMK DDD FV+VD VL+
Sbjct: 485 ELKKEAEFFGDIVIVPYMDSYDLVVLKTVAICEYGAHQLAAKFIMKCDDDTFVQVDAVLS 544

Query: 527 SLKRIDVQSGLLYGLINSDSQPHRDPESKWYISMEEWPEDNYPTWAHGPGYVVSNDIAKT 586
             K+      L  G IN   +P R  + KW ++ EEWPE++YP +A+GPGY++SNDI++ 
Sbjct: 545 EAKKTPTDRSLYIGNINYYHKPLR--QGKWSVTYEEWPEEDYPPYANGPGYILSNDISRF 604

Query: 587 ISKKYKEGRLKMFKLEDVAMGIWIENMKREGLKIRYEKDDRIHIEGCKDDYMVAHYQGPR 628
           I K++++ +L+MFK+EDV++G+W+E        + Y    R    GC ++Y+ AHYQ PR
Sbjct: 605 IVKEFEKHKLRMFKMEDVSVGMWVEQFNNGTKPVDYIHSLRFCQFGCIENYLTAHYQSPR 664

BLAST of Tan0009873 vs. ExPASy Swiss-Prot
Match: Q8GXG6 (Hydroxyproline O-galactosyltransferase GALT4 OS=Arabidopsis thaliana OX=3702 GN=GALT4 PE=2 SV=2)

HSP 1 Score: 341.3 bits (874), Expect = 2.4e-92
Identity = 196/545 (35.96%), Postives = 300/545 (55.05%), Query Frame = 0

Query: 107 AVEAIKEAGGVWDVLKTSIEKERLGYTNESARAKEKQCPHFLTKMNATKLDNNGHKLRMP 166
           A  AI     +WD L + + K          + + ++CP  +  ++ ++  N    L +P
Sbjct: 136 AKHAISMGRKMWDGLDSGLIKP----DKAPVKTRIEKCPD-MVSVSESEFVNRSRILVLP 195

Query: 167 CGLTQGSSITVIGIP-------DG----LLGNFRIDLTGEPLPGEPDPPLILHYNVRLLG 226
           CGLT GS ITV+  P       DG    ++  F ++L G       DPP ILH+N R+ G
Sbjct: 196 CGLTLGSHITVVATPHWAHVEKDGDKTAMVSQFMMELQGLKAVDGEDPPRILHFNPRIKG 255

Query: 227 DKLTEDPVIVQNTWTVSRDWGDEERCPS-PGSDENGKVDELEKCNKIV------GNVETR 286
           D  +  PVI QNT      WG   RC     SD+   VD   KC +        GN    
Sbjct: 256 D-WSGRPVIEQNT-CYRMQWGSGLRCDGRESSDDEEYVDGEVKCERWKRDDDDGGNNGDD 315

Query: 287 LSESNKNFNKSKSMVQGAKTRPY---FPFKLGQPFAATLRVGVDGIQMTVDGKHVTSFAY 346
             ES K +  ++ M +  K   +   +PF  G+ F  TLR G++G  ++V+G+H+TSF Y
Sbjct: 316 FDESKKTWWLNRLMGRRKKMITHDWDYPFAEGKLFVLTLRAGMEGYHISVNGRHITSFPY 375

Query: 347 RETLEPWLVSEVKISGDLKLISVLASGLPTSEDS---DHIVNIEALKSSPLSLERPLDLF 406
           R        + + + G++ + SV A+ LP++  S      + ++ +  +P   ++P++LF
Sbjct: 376 RTGFVLEDATGLAVKGNIDVHSVYAASLPSTNPSFAPQKHLEMQRIWKAPSLPQKPVELF 435

Query: 407 IGVFSTANNFKYRMAVRRTWIQYPEVRSGSVAVRFFVGLHKNQIVNEELWDEARTYGDIQ 466
           IG+ S  N+F  RMAVR++W+Q   VRS  V  RFFV LH  + VN +L  EA  +GDI 
Sbjct: 436 IGILSAGNHFAERMAVRKSWMQQKLVRSSKVVARFFVALHARKEVNVDLKKEAEYFGDIV 495

Query: 467 MMPFVDYYSLITWKTLGICIFGAEIASAKYIMKTDDDAFVRVDEVLASLKRIDVQSGLLY 526
           ++P++D+Y L+  KT+ IC +G    +AKY+MK DDD FVRVD V+   +++  +  L  
Sbjct: 496 IVPYMDHYDLVVLKTVAICEYGVNTVAAKYVMKCDDDTFVRVDAVIQEAEKVKGRESLYI 555

Query: 527 GLINSDSQPHRDPESKWYISMEEWPEDNYPTWAHGPGYVVSNDIAKTISKKYKEGRLKMF 586
           G IN + +P R    KW ++ EEWPE+ YP +A+GPGY++S D+AK I   +++ RL++F
Sbjct: 556 GNINFNHKPLR--TGKWAVTFEEWPEEYYPPYANGPGYILSYDVAKFIVDDFEQKRLRLF 615

Query: 587 KLEDVAMGIWIENMKREGLKIRYEKDDRIHIEGCKDDYMVAHYQGPREMLCLWQKLQEGN 628
           K+EDV+MG+W+E    E   +      +    GC +DY  AHYQ PR+M+C+W KLQ   
Sbjct: 616 KMEDVSMGMWVEKF-NETRPVAVVHSLKFCQFGCIEDYFTAHYQSPRQMICMWDKLQRLG 670

BLAST of Tan0009873 vs. NCBI nr
Match: KAG7013139.1 (Beta-1,3-galactosyltransferase GALT1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1230.7 bits (3183), Expect = 0.0e+00
Identity = 593/631 (93.98%), Postives = 615/631 (97.46%), Query Frame = 0

Query: 1   MKKSYGRVLLASLFMLVLMVYVILRSSTRQDYYATPLYTSVDPLQWLNPAVPPAVQNPEI 60
           MKK+YGRVL+ASL ML+LMVYVI RSSTRQ+YYATPLY +VDPLQWLNPAVP AVQNPEI
Sbjct: 1   MKKNYGRVLVASLSMLLLMVYVISRSSTRQNYYATPLYNNVDPLQWLNPAVPTAVQNPEI 60

Query: 61  GHQVISADSIISSLFTQGNFTDGENRALQTWNHLRSIINCTRGLPNAVEAIKEAGGVWDV 120
            +QVISADSIISSLFT GNFTDGENRALQTWNHLR+IIN TRGLPNAVEAIKEAGGVWD+
Sbjct: 61  AYQVISADSIISSLFTPGNFTDGENRALQTWNHLRTIINHTRGLPNAVEAIKEAGGVWDI 120

Query: 121 LKTSIEKERLGYTNESARAKEKQCPHFLTKMNATKLDNNGHKLRMPCGLTQGSSITVIGI 180
           LK SIEKERLGYTN SARAKEKQCPHFLTKMNATKL++NGHKLRMPCGLTQGSSIT+IGI
Sbjct: 121 LKASIEKERLGYTNGSARAKEKQCPHFLTKMNATKLEHNGHKLRMPCGLTQGSSITIIGI 180

Query: 181 PDGLLGNFRIDLTGEPLPGEPDPPLILHYNVRLLGDKLTEDPVIVQNTWTVSRDWGDEER 240
           PDGLLGNFRIDLTGEPLPGEPDPPLILHYNVRLLGDKLTEDPVIVQNTWTVS DWGDEER
Sbjct: 181 PDGLLGNFRIDLTGEPLPGEPDPPLILHYNVRLLGDKLTEDPVIVQNTWTVSHDWGDEER 240

Query: 241 CPSPGSDENGKVDELEKCNKIVGNVETRLSESNKNFNKSKSMVQGAKTRPYFPFKLGQPF 300
           CPSP SDENGKVDELEKCNKIVGNVETRLS+SNKNFNKSKSMVQGAKTRPYFPFKLGQPF
Sbjct: 241 CPSPDSDENGKVDELEKCNKIVGNVETRLSDSNKNFNKSKSMVQGAKTRPYFPFKLGQPF 300

Query: 301 AATLRVGVDGIQMTVDGKHVTSFAYRETLEPWLVSEVKISGDLKLISVLASGLPTSEDSD 360
           AATLRVGVDGIQMTVDGKHVTSFAYRETLEPWLVSEVKISGDLKLISVLASGLPTSEDSD
Sbjct: 301 AATLRVGVDGIQMTVDGKHVTSFAYRETLEPWLVSEVKISGDLKLISVLASGLPTSEDSD 360

Query: 361 HIVNIEALKSSPLSLERPLDLFIGVFSTANNFKYRMAVRRTWIQYPEVRSGSVAVRFFVG 420
           HIVNIEALKSSPLS E+PLDLFIGVFSTANNFKYRMA+RRTW+QYP V+SGSVAVRFFVG
Sbjct: 361 HIVNIEALKSSPLSPEKPLDLFIGVFSTANNFKYRMAIRRTWMQYPAVQSGSVAVRFFVG 420

Query: 421 LHKNQIVNEELWDEARTYGDIQMMPFVDYYSLITWKTLGICIFGAEIASAKYIMKTDDDA 480
           LHKNQIVNEELWDEARTYGDIQMMPFVDYYS+ITWKTLGICIFGAEIASAKYIMKTDDDA
Sbjct: 421 LHKNQIVNEELWDEARTYGDIQMMPFVDYYSVITWKTLGICIFGAEIASAKYIMKTDDDA 480

Query: 481 FVRVDEVLASLKRIDVQSGLLYGLINSDSQPHRDPESKWYISMEEWPEDNYPTWAHGPGY 540
           FVRVDEVLASLKRID QSGLLYGLINSDSQPHRDPESKWYISME WPEDNYPTWAHGPGY
Sbjct: 481 FVRVDEVLASLKRIDAQSGLLYGLINSDSQPHRDPESKWYISMEAWPEDNYPTWAHGPGY 540

Query: 541 VVSNDIAKTISKKYKEGRLKMFKLEDVAMGIWIENMKREGLKIRYEKDDRIHIEGCKDDY 600
           VVSNDIAKTISK YKEGRLKMFKLEDVAMGIWIENMK++GLK++YEKDDRIHIEGCKDDY
Sbjct: 541 VVSNDIAKTISKNYKEGRLKMFKLEDVAMGIWIENMKKDGLKVQYEKDDRIHIEGCKDDY 600

Query: 601 MVAHYQGPREMLCLWQKLQEGNGIRCCGSDN 632
           +VAHYQGPREMLCLWQKLQEGNGIRCCGSDN
Sbjct: 601 VVAHYQGPREMLCLWQKLQEGNGIRCCGSDN 631

BLAST of Tan0009873 vs. NCBI nr
Match: XP_022968057.1 (beta-1,3-galactosyltransferase GALT1-like [Cucurbita maxima])

HSP 1 Score: 1229.9 bits (3181), Expect = 0.0e+00
Identity = 595/631 (94.29%), Postives = 614/631 (97.31%), Query Frame = 0

Query: 1   MKKSYGRVLLASLFMLVLMVYVILRSSTRQDYYATPLYTSVDPLQWLNPAVPPAVQNPEI 60
           MKKSYGRVL+ASL ML+LMVYVI RSSTRQ+YYATPLY SV PLQWLNPAVP AVQNPEI
Sbjct: 1   MKKSYGRVLVASLSMLLLMVYVISRSSTRQNYYATPLYNSVAPLQWLNPAVPTAVQNPEI 60

Query: 61  GHQVISADSIISSLFTQGNFTDGENRALQTWNHLRSIINCTRGLPNAVEAIKEAGGVWDV 120
            +QVISADSIISSLFT GNFTDGENRALQTWNHLR+IIN TRGLPNAVEAIKEAGGVWD+
Sbjct: 61  AYQVISADSIISSLFTPGNFTDGENRALQTWNHLRTIINHTRGLPNAVEAIKEAGGVWDI 120

Query: 121 LKTSIEKERLGYTNESARAKEKQCPHFLTKMNATKLDNNGHKLRMPCGLTQGSSITVIGI 180
           LK SIEKERLGY N SARAKEKQCPHFLTKMNATKL++NGHKLRMPCGLTQGSSITVIGI
Sbjct: 121 LKASIEKERLGYINGSARAKEKQCPHFLTKMNATKLEHNGHKLRMPCGLTQGSSITVIGI 180

Query: 181 PDGLLGNFRIDLTGEPLPGEPDPPLILHYNVRLLGDKLTEDPVIVQNTWTVSRDWGDEER 240
           PDGLLGNFRIDLTGEPLPGEPDPPLILHYNVRLLGDKLTEDPVIVQNTWTVS DWGDEER
Sbjct: 181 PDGLLGNFRIDLTGEPLPGEPDPPLILHYNVRLLGDKLTEDPVIVQNTWTVSHDWGDEER 240

Query: 241 CPSPGSDENGKVDELEKCNKIVGNVETRLSESNKNFNKSKSMVQGAKTRPYFPFKLGQPF 300
           CPSPGSDENGKVDELEKCNKIVGNVETRLS+SNKNFNKSKSMVQG+KTRPYFPFKLGQPF
Sbjct: 241 CPSPGSDENGKVDELEKCNKIVGNVETRLSDSNKNFNKSKSMVQGSKTRPYFPFKLGQPF 300

Query: 301 AATLRVGVDGIQMTVDGKHVTSFAYRETLEPWLVSEVKISGDLKLISVLASGLPTSEDSD 360
           AATLRVGVDGIQMTVDGKHVTSFAYRETLEPWLVSEVKISGDLKLISVLASGLPTSEDSD
Sbjct: 301 AATLRVGVDGIQMTVDGKHVTSFAYRETLEPWLVSEVKISGDLKLISVLASGLPTSEDSD 360

Query: 361 HIVNIEALKSSPLSLERPLDLFIGVFSTANNFKYRMAVRRTWIQYPEVRSGSVAVRFFVG 420
           HIVNIEALKSSPLS E+PLDLFIGVFSTANNFKYRMA+RRTW+QYP V+SGSVAVRFFVG
Sbjct: 361 HIVNIEALKSSPLSPEKPLDLFIGVFSTANNFKYRMAIRRTWMQYPAVQSGSVAVRFFVG 420

Query: 421 LHKNQIVNEELWDEARTYGDIQMMPFVDYYSLITWKTLGICIFGAEIASAKYIMKTDDDA 480
           LHKNQIVNEELWDEARTYGDIQMMPFVDYYS+ITWKTLGICIFGAEIASAKYIMKTDDDA
Sbjct: 421 LHKNQIVNEELWDEARTYGDIQMMPFVDYYSVITWKTLGICIFGAEIASAKYIMKTDDDA 480

Query: 481 FVRVDEVLASLKRIDVQSGLLYGLINSDSQPHRDPESKWYISMEEWPEDNYPTWAHGPGY 540
           FVRVDEVLASLKRID QSGLLYGLINSDSQPHRDPESKWYISME WPEDNYPTWAHGPGY
Sbjct: 481 FVRVDEVLASLKRIDAQSGLLYGLINSDSQPHRDPESKWYISMEAWPEDNYPTWAHGPGY 540

Query: 541 VVSNDIAKTISKKYKEGRLKMFKLEDVAMGIWIENMKREGLKIRYEKDDRIHIEGCKDDY 600
           VVSNDIAKTISK YKEGRLKMFKLEDVAMGIWIENMK++GLKI+YEKDDRIHIEGCKDDY
Sbjct: 541 VVSNDIAKTISKNYKEGRLKMFKLEDVAMGIWIENMKKDGLKIQYEKDDRIHIEGCKDDY 600

Query: 601 MVAHYQGPREMLCLWQKLQEGNGIRCCGSDN 632
           +VAHYQGPREMLCLWQKLQEGNGIRCCGSDN
Sbjct: 601 VVAHYQGPREMLCLWQKLQEGNGIRCCGSDN 631

BLAST of Tan0009873 vs. NCBI nr
Match: KAG6574080.1 (Beta-1,3-galactosyltransferase GALT1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1228.8 bits (3178), Expect = 0.0e+00
Identity = 592/631 (93.82%), Postives = 614/631 (97.31%), Query Frame = 0

Query: 1   MKKSYGRVLLASLFMLVLMVYVILRSSTRQDYYATPLYTSVDPLQWLNPAVPPAVQNPEI 60
           MKK+YGRVL+ASL ML+LMVYVI RSSTRQ+YYATPLY +VDPLQWLNPAVP AVQNPEI
Sbjct: 1   MKKNYGRVLVASLSMLLLMVYVISRSSTRQNYYATPLYNNVDPLQWLNPAVPTAVQNPEI 60

Query: 61  GHQVISADSIISSLFTQGNFTDGENRALQTWNHLRSIINCTRGLPNAVEAIKEAGGVWDV 120
            +QVISADSIISSLFT GNFTDGENRAL TWNHLR+IIN TRGLPNAVEAIKEAGGVWD+
Sbjct: 61  AYQVISADSIISSLFTPGNFTDGENRALHTWNHLRTIINHTRGLPNAVEAIKEAGGVWDI 120

Query: 121 LKTSIEKERLGYTNESARAKEKQCPHFLTKMNATKLDNNGHKLRMPCGLTQGSSITVIGI 180
           LK SIEKERLGYTN SARAKEKQCPHFLTKMNATKL++NGHKLRMPCGLTQGSSIT+IGI
Sbjct: 121 LKASIEKERLGYTNGSARAKEKQCPHFLTKMNATKLEHNGHKLRMPCGLTQGSSITIIGI 180

Query: 181 PDGLLGNFRIDLTGEPLPGEPDPPLILHYNVRLLGDKLTEDPVIVQNTWTVSRDWGDEER 240
           PDGLLGNFRIDLTGEPLPGEPDPPLILHYNVRLLGDKLTEDPVIVQNTWTVS DWGDEER
Sbjct: 181 PDGLLGNFRIDLTGEPLPGEPDPPLILHYNVRLLGDKLTEDPVIVQNTWTVSHDWGDEER 240

Query: 241 CPSPGSDENGKVDELEKCNKIVGNVETRLSESNKNFNKSKSMVQGAKTRPYFPFKLGQPF 300
           CPSP SDENGKVDELEKCNKIVGNVETRLS+SNKNFNKSKSMVQGAKTRPYFPFKLGQPF
Sbjct: 241 CPSPDSDENGKVDELEKCNKIVGNVETRLSDSNKNFNKSKSMVQGAKTRPYFPFKLGQPF 300

Query: 301 AATLRVGVDGIQMTVDGKHVTSFAYRETLEPWLVSEVKISGDLKLISVLASGLPTSEDSD 360
           AATLRVGVDGIQMTVDGKHVTSFAYRETLEPWLVSEVKISGDLKLISVLASGLPTSEDSD
Sbjct: 301 AATLRVGVDGIQMTVDGKHVTSFAYRETLEPWLVSEVKISGDLKLISVLASGLPTSEDSD 360

Query: 361 HIVNIEALKSSPLSLERPLDLFIGVFSTANNFKYRMAVRRTWIQYPEVRSGSVAVRFFVG 420
           HIVNIEALKSSPLS E+PLDLFIGVFSTANNFKYRMA+RRTW+QYP V+SGSVAVRFFVG
Sbjct: 361 HIVNIEALKSSPLSPEKPLDLFIGVFSTANNFKYRMAIRRTWMQYPAVQSGSVAVRFFVG 420

Query: 421 LHKNQIVNEELWDEARTYGDIQMMPFVDYYSLITWKTLGICIFGAEIASAKYIMKTDDDA 480
           LHKNQIVNEELWDEARTYGDIQMMPFVDYYS+ITWKTLGICIFGAEIASAKYIMKTDDDA
Sbjct: 421 LHKNQIVNEELWDEARTYGDIQMMPFVDYYSVITWKTLGICIFGAEIASAKYIMKTDDDA 480

Query: 481 FVRVDEVLASLKRIDVQSGLLYGLINSDSQPHRDPESKWYISMEEWPEDNYPTWAHGPGY 540
           FVRVDEVLASLKRID QSGLLYGLINSDSQPHRDPESKWYISME WPEDNYPTWAHGPGY
Sbjct: 481 FVRVDEVLASLKRIDAQSGLLYGLINSDSQPHRDPESKWYISMEAWPEDNYPTWAHGPGY 540

Query: 541 VVSNDIAKTISKKYKEGRLKMFKLEDVAMGIWIENMKREGLKIRYEKDDRIHIEGCKDDY 600
           VVSNDIAKTISK YKEGRLKMFKLEDVAMGIWIENMK++GLK++YEKDDRIHIEGCKDDY
Sbjct: 541 VVSNDIAKTISKNYKEGRLKMFKLEDVAMGIWIENMKKDGLKVQYEKDDRIHIEGCKDDY 600

Query: 601 MVAHYQGPREMLCLWQKLQEGNGIRCCGSDN 632
           +VAHYQGPREMLCLWQKLQEGNGIRCCGSDN
Sbjct: 601 VVAHYQGPREMLCLWQKLQEGNGIRCCGSDN 631

BLAST of Tan0009873 vs. NCBI nr
Match: XP_022945449.1 (beta-1,3-galactosyltransferase GALT1-like [Cucurbita moschata])

HSP 1 Score: 1224.5 bits (3167), Expect = 0.0e+00
Identity = 591/631 (93.66%), Postives = 612/631 (96.99%), Query Frame = 0

Query: 1   MKKSYGRVLLASLFMLVLMVYVILRSSTRQDYYATPLYTSVDPLQWLNPAVPPAVQNPEI 60
           MKK+YGRVL+ASL ML+LMVYVI RSSTRQ+YYATPLY SVDPLQWLNPAVP AVQNPEI
Sbjct: 1   MKKNYGRVLVASLSMLLLMVYVISRSSTRQNYYATPLYNSVDPLQWLNPAVPTAVQNPEI 60

Query: 61  GHQVISADSIISSLFTQGNFTDGENRALQTWNHLRSIINCTRGLPNAVEAIKEAGGVWDV 120
            +QVISADSIISSLFT GNFTDGEN+ALQTWNHL++IIN TRGLPN+VEAIKEAGGVWD+
Sbjct: 61  AYQVISADSIISSLFTPGNFTDGENQALQTWNHLKTIINHTRGLPNSVEAIKEAGGVWDI 120

Query: 121 LKTSIEKERLGYTNESARAKEKQCPHFLTKMNATKLDNNGHKLRMPCGLTQGSSITVIGI 180
           LK SIEKERLGYTN SAR KEKQCPHFLTKMNATKLD+NGHKLRMPCGLTQGSSITVIGI
Sbjct: 121 LKASIEKERLGYTNGSARVKEKQCPHFLTKMNATKLDHNGHKLRMPCGLTQGSSITVIGI 180

Query: 181 PDGLLGNFRIDLTGEPLPGEPDPPLILHYNVRLLGDKLTEDPVIVQNTWTVSRDWGDEER 240
           PDGLLGNFRIDLTGEPLPGEPDPPLILHYNVRLLGDKLTEDPVIVQNTWTVS DWGDEER
Sbjct: 181 PDGLLGNFRIDLTGEPLPGEPDPPLILHYNVRLLGDKLTEDPVIVQNTWTVSHDWGDEER 240

Query: 241 CPSPGSDENGKVDELEKCNKIVGNVETRLSESNKNFNKSKSMVQGAKTRPYFPFKLGQPF 300
           CPSP SDENGKVDELEKCNKIVGNVETRLS SNKNFNKSKSMVQGAKTRPYFPFKLGQPF
Sbjct: 241 CPSPDSDENGKVDELEKCNKIVGNVETRLSGSNKNFNKSKSMVQGAKTRPYFPFKLGQPF 300

Query: 301 AATLRVGVDGIQMTVDGKHVTSFAYRETLEPWLVSEVKISGDLKLISVLASGLPTSEDSD 360
           AATLRVGVDGIQMTVDGKHVTSFAYRETLEPWLVSEVKISGDLKLISVLASGLPTSEDSD
Sbjct: 301 AATLRVGVDGIQMTVDGKHVTSFAYRETLEPWLVSEVKISGDLKLISVLASGLPTSEDSD 360

Query: 361 HIVNIEALKSSPLSLERPLDLFIGVFSTANNFKYRMAVRRTWIQYPEVRSGSVAVRFFVG 420
           HIVNIEALKSSPLS  +PLDLFIGVFSTANNFKYRMA+RRTW+QYP V+SGSVAVRFFVG
Sbjct: 361 HIVNIEALKSSPLSPAKPLDLFIGVFSTANNFKYRMAIRRTWMQYPAVQSGSVAVRFFVG 420

Query: 421 LHKNQIVNEELWDEARTYGDIQMMPFVDYYSLITWKTLGICIFGAEIASAKYIMKTDDDA 480
           LHKNQIVNEELWDEARTYGDIQMMPFVDYYS+ITWKTLGICIFGAEIASAKYIMKTDDDA
Sbjct: 421 LHKNQIVNEELWDEARTYGDIQMMPFVDYYSVITWKTLGICIFGAEIASAKYIMKTDDDA 480

Query: 481 FVRVDEVLASLKRIDVQSGLLYGLINSDSQPHRDPESKWYISMEEWPEDNYPTWAHGPGY 540
           FVRVDEVLASLKRID QSGLLYGLINSDSQPHRDPESKWYISME WPEDNYPTWAHGPGY
Sbjct: 481 FVRVDEVLASLKRIDAQSGLLYGLINSDSQPHRDPESKWYISMEAWPEDNYPTWAHGPGY 540

Query: 541 VVSNDIAKTISKKYKEGRLKMFKLEDVAMGIWIENMKREGLKIRYEKDDRIHIEGCKDDY 600
           VVSNDIAKTISK YKEGRLKMFKLEDVAMGIWIENMK++GLK++YEKDDRIHIEGCKDDY
Sbjct: 541 VVSNDIAKTISKNYKEGRLKMFKLEDVAMGIWIENMKKDGLKVQYEKDDRIHIEGCKDDY 600

Query: 601 MVAHYQGPREMLCLWQKLQEGNGIRCCGSDN 632
           +VAHYQGPREMLCLWQKLQEGNGIRCCGSDN
Sbjct: 601 VVAHYQGPREMLCLWQKLQEGNGIRCCGSDN 631

BLAST of Tan0009873 vs. NCBI nr
Match: XP_023541817.1 (beta-1,3-galactosyltransferase GALT1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1221.8 bits (3160), Expect = 0.0e+00
Identity = 592/631 (93.82%), Postives = 611/631 (96.83%), Query Frame = 0

Query: 1   MKKSYGRVLLASLFMLVLMVYVILRSSTRQDYYATPLYTSVDPLQWLNPAVPPAVQNPEI 60
           MKK+YG VL+ASL ML+LMVYVI RSSTRQ+YYATPLY SVDPL+WLNPAVP AVQNPEI
Sbjct: 1   MKKNYGWVLVASLSMLLLMVYVISRSSTRQNYYATPLYNSVDPLEWLNPAVPTAVQNPEI 60

Query: 61  GHQVISADSIISSLFTQGNFTDGENRALQTWNHLRSIINCTRGLPNAVEAIKEAGGVWDV 120
            +QVISADSIISSLFT GNFTDGENRALQTWNHLR+IIN TRGLPNAVEAIKEAGGVWD+
Sbjct: 61  AYQVISADSIISSLFTPGNFTDGENRALQTWNHLRTIINHTRGLPNAVEAIKEAGGVWDI 120

Query: 121 LKTSIEKERLGYTNESARAKEKQCPHFLTKMNATKLDNNGHKLRMPCGLTQGSSITVIGI 180
           LK SIEKERLGYTN SARAKEKQCPHFLTKMNATKL++NGHKLRMPCGLTQGSSITVIGI
Sbjct: 121 LKASIEKERLGYTNGSARAKEKQCPHFLTKMNATKLEHNGHKLRMPCGLTQGSSITVIGI 180

Query: 181 PDGLLGNFRIDLTGEPLPGEPDPPLILHYNVRLLGDKLTEDPVIVQNTWTVSRDWGDEER 240
           PDGLLGNFRIDLTGEPLPGEPDPPLILHYNVRLLGDKLTEDPVIVQNTWTVS DWGDEER
Sbjct: 181 PDGLLGNFRIDLTGEPLPGEPDPPLILHYNVRLLGDKLTEDPVIVQNTWTVSHDWGDEER 240

Query: 241 CPSPGSDENGKVDELEKCNKIVGNVETRLSESNKNFNKSKSMVQGAKTRPYFPFKLGQPF 300
           CPSP SDENGKVDELEKCNKIVGNVETRLS SNKNFNKSKSMVQG KTRPYFPFKLGQPF
Sbjct: 241 CPSPDSDENGKVDELEKCNKIVGNVETRLSGSNKNFNKSKSMVQGDKTRPYFPFKLGQPF 300

Query: 301 AATLRVGVDGIQMTVDGKHVTSFAYRETLEPWLVSEVKISGDLKLISVLASGLPTSEDSD 360
           AATLRVGVDGIQMTVDGKHVTSFAYRETLEPWLVSEVKISGDLKLISVLASGLPTSEDSD
Sbjct: 301 AATLRVGVDGIQMTVDGKHVTSFAYRETLEPWLVSEVKISGDLKLISVLASGLPTSEDSD 360

Query: 361 HIVNIEALKSSPLSLERPLDLFIGVFSTANNFKYRMAVRRTWIQYPEVRSGSVAVRFFVG 420
           HIVNIEALKSSPLS E+PLDLFIGVFSTANNFKYRMA+RRTW+QYP V+SGSVAVRFFVG
Sbjct: 361 HIVNIEALKSSPLSPEKPLDLFIGVFSTANNFKYRMAIRRTWMQYPAVQSGSVAVRFFVG 420

Query: 421 LHKNQIVNEELWDEARTYGDIQMMPFVDYYSLITWKTLGICIFGAEIASAKYIMKTDDDA 480
           LHKNQIVNEELWDEARTYGDIQMMPFVDYYS+ITWKTLGICIFGAEIASAKYIMKTDDDA
Sbjct: 421 LHKNQIVNEELWDEARTYGDIQMMPFVDYYSVITWKTLGICIFGAEIASAKYIMKTDDDA 480

Query: 481 FVRVDEVLASLKRIDVQSGLLYGLINSDSQPHRDPESKWYISMEEWPEDNYPTWAHGPGY 540
           FVRVDEVLASLKRID QSGLLYGLINSDSQPHRDPESKWYISME WPEDNYPTWAHGPGY
Sbjct: 481 FVRVDEVLASLKRIDAQSGLLYGLINSDSQPHRDPESKWYISMEAWPEDNYPTWAHGPGY 540

Query: 541 VVSNDIAKTISKKYKEGRLKMFKLEDVAMGIWIENMKREGLKIRYEKDDRIHIEGCKDDY 600
           VVSNDIAKTISK YKE RLKMFKLEDVAMGIWIENMK++GLKI+YEKDDRIHIEGCKDDY
Sbjct: 541 VVSNDIAKTISKNYKEDRLKMFKLEDVAMGIWIENMKKDGLKIQYEKDDRIHIEGCKDDY 600

Query: 601 MVAHYQGPREMLCLWQKLQEGNGIRCCGSDN 632
           +VAHYQGPREMLCLWQKLQEGNGIRCCGSDN
Sbjct: 601 VVAHYQGPREMLCLWQKLQEGNGIRCCGSDN 631

BLAST of Tan0009873 vs. ExPASy TrEMBL
Match: A0A6J1HSG4 (beta-1,3-galactosyltransferase GALT1-like OS=Cucurbita maxima OX=3661 GN=LOC111467410 PE=3 SV=1)

HSP 1 Score: 1229.9 bits (3181), Expect = 0.0e+00
Identity = 595/631 (94.29%), Postives = 614/631 (97.31%), Query Frame = 0

Query: 1   MKKSYGRVLLASLFMLVLMVYVILRSSTRQDYYATPLYTSVDPLQWLNPAVPPAVQNPEI 60
           MKKSYGRVL+ASL ML+LMVYVI RSSTRQ+YYATPLY SV PLQWLNPAVP AVQNPEI
Sbjct: 1   MKKSYGRVLVASLSMLLLMVYVISRSSTRQNYYATPLYNSVAPLQWLNPAVPTAVQNPEI 60

Query: 61  GHQVISADSIISSLFTQGNFTDGENRALQTWNHLRSIINCTRGLPNAVEAIKEAGGVWDV 120
            +QVISADSIISSLFT GNFTDGENRALQTWNHLR+IIN TRGLPNAVEAIKEAGGVWD+
Sbjct: 61  AYQVISADSIISSLFTPGNFTDGENRALQTWNHLRTIINHTRGLPNAVEAIKEAGGVWDI 120

Query: 121 LKTSIEKERLGYTNESARAKEKQCPHFLTKMNATKLDNNGHKLRMPCGLTQGSSITVIGI 180
           LK SIEKERLGY N SARAKEKQCPHFLTKMNATKL++NGHKLRMPCGLTQGSSITVIGI
Sbjct: 121 LKASIEKERLGYINGSARAKEKQCPHFLTKMNATKLEHNGHKLRMPCGLTQGSSITVIGI 180

Query: 181 PDGLLGNFRIDLTGEPLPGEPDPPLILHYNVRLLGDKLTEDPVIVQNTWTVSRDWGDEER 240
           PDGLLGNFRIDLTGEPLPGEPDPPLILHYNVRLLGDKLTEDPVIVQNTWTVS DWGDEER
Sbjct: 181 PDGLLGNFRIDLTGEPLPGEPDPPLILHYNVRLLGDKLTEDPVIVQNTWTVSHDWGDEER 240

Query: 241 CPSPGSDENGKVDELEKCNKIVGNVETRLSESNKNFNKSKSMVQGAKTRPYFPFKLGQPF 300
           CPSPGSDENGKVDELEKCNKIVGNVETRLS+SNKNFNKSKSMVQG+KTRPYFPFKLGQPF
Sbjct: 241 CPSPGSDENGKVDELEKCNKIVGNVETRLSDSNKNFNKSKSMVQGSKTRPYFPFKLGQPF 300

Query: 301 AATLRVGVDGIQMTVDGKHVTSFAYRETLEPWLVSEVKISGDLKLISVLASGLPTSEDSD 360
           AATLRVGVDGIQMTVDGKHVTSFAYRETLEPWLVSEVKISGDLKLISVLASGLPTSEDSD
Sbjct: 301 AATLRVGVDGIQMTVDGKHVTSFAYRETLEPWLVSEVKISGDLKLISVLASGLPTSEDSD 360

Query: 361 HIVNIEALKSSPLSLERPLDLFIGVFSTANNFKYRMAVRRTWIQYPEVRSGSVAVRFFVG 420
           HIVNIEALKSSPLS E+PLDLFIGVFSTANNFKYRMA+RRTW+QYP V+SGSVAVRFFVG
Sbjct: 361 HIVNIEALKSSPLSPEKPLDLFIGVFSTANNFKYRMAIRRTWMQYPAVQSGSVAVRFFVG 420

Query: 421 LHKNQIVNEELWDEARTYGDIQMMPFVDYYSLITWKTLGICIFGAEIASAKYIMKTDDDA 480
           LHKNQIVNEELWDEARTYGDIQMMPFVDYYS+ITWKTLGICIFGAEIASAKYIMKTDDDA
Sbjct: 421 LHKNQIVNEELWDEARTYGDIQMMPFVDYYSVITWKTLGICIFGAEIASAKYIMKTDDDA 480

Query: 481 FVRVDEVLASLKRIDVQSGLLYGLINSDSQPHRDPESKWYISMEEWPEDNYPTWAHGPGY 540
           FVRVDEVLASLKRID QSGLLYGLINSDSQPHRDPESKWYISME WPEDNYPTWAHGPGY
Sbjct: 481 FVRVDEVLASLKRIDAQSGLLYGLINSDSQPHRDPESKWYISMEAWPEDNYPTWAHGPGY 540

Query: 541 VVSNDIAKTISKKYKEGRLKMFKLEDVAMGIWIENMKREGLKIRYEKDDRIHIEGCKDDY 600
           VVSNDIAKTISK YKEGRLKMFKLEDVAMGIWIENMK++GLKI+YEKDDRIHIEGCKDDY
Sbjct: 541 VVSNDIAKTISKNYKEGRLKMFKLEDVAMGIWIENMKKDGLKIQYEKDDRIHIEGCKDDY 600

Query: 601 MVAHYQGPREMLCLWQKLQEGNGIRCCGSDN 632
           +VAHYQGPREMLCLWQKLQEGNGIRCCGSDN
Sbjct: 601 VVAHYQGPREMLCLWQKLQEGNGIRCCGSDN 631

BLAST of Tan0009873 vs. ExPASy TrEMBL
Match: A0A6J1G100 (beta-1,3-galactosyltransferase GALT1-like OS=Cucurbita moschata OX=3662 GN=LOC111449679 PE=3 SV=1)

HSP 1 Score: 1224.5 bits (3167), Expect = 0.0e+00
Identity = 591/631 (93.66%), Postives = 612/631 (96.99%), Query Frame = 0

Query: 1   MKKSYGRVLLASLFMLVLMVYVILRSSTRQDYYATPLYTSVDPLQWLNPAVPPAVQNPEI 60
           MKK+YGRVL+ASL ML+LMVYVI RSSTRQ+YYATPLY SVDPLQWLNPAVP AVQNPEI
Sbjct: 1   MKKNYGRVLVASLSMLLLMVYVISRSSTRQNYYATPLYNSVDPLQWLNPAVPTAVQNPEI 60

Query: 61  GHQVISADSIISSLFTQGNFTDGENRALQTWNHLRSIINCTRGLPNAVEAIKEAGGVWDV 120
            +QVISADSIISSLFT GNFTDGEN+ALQTWNHL++IIN TRGLPN+VEAIKEAGGVWD+
Sbjct: 61  AYQVISADSIISSLFTPGNFTDGENQALQTWNHLKTIINHTRGLPNSVEAIKEAGGVWDI 120

Query: 121 LKTSIEKERLGYTNESARAKEKQCPHFLTKMNATKLDNNGHKLRMPCGLTQGSSITVIGI 180
           LK SIEKERLGYTN SAR KEKQCPHFLTKMNATKLD+NGHKLRMPCGLTQGSSITVIGI
Sbjct: 121 LKASIEKERLGYTNGSARVKEKQCPHFLTKMNATKLDHNGHKLRMPCGLTQGSSITVIGI 180

Query: 181 PDGLLGNFRIDLTGEPLPGEPDPPLILHYNVRLLGDKLTEDPVIVQNTWTVSRDWGDEER 240
           PDGLLGNFRIDLTGEPLPGEPDPPLILHYNVRLLGDKLTEDPVIVQNTWTVS DWGDEER
Sbjct: 181 PDGLLGNFRIDLTGEPLPGEPDPPLILHYNVRLLGDKLTEDPVIVQNTWTVSHDWGDEER 240

Query: 241 CPSPGSDENGKVDELEKCNKIVGNVETRLSESNKNFNKSKSMVQGAKTRPYFPFKLGQPF 300
           CPSP SDENGKVDELEKCNKIVGNVETRLS SNKNFNKSKSMVQGAKTRPYFPFKLGQPF
Sbjct: 241 CPSPDSDENGKVDELEKCNKIVGNVETRLSGSNKNFNKSKSMVQGAKTRPYFPFKLGQPF 300

Query: 301 AATLRVGVDGIQMTVDGKHVTSFAYRETLEPWLVSEVKISGDLKLISVLASGLPTSEDSD 360
           AATLRVGVDGIQMTVDGKHVTSFAYRETLEPWLVSEVKISGDLKLISVLASGLPTSEDSD
Sbjct: 301 AATLRVGVDGIQMTVDGKHVTSFAYRETLEPWLVSEVKISGDLKLISVLASGLPTSEDSD 360

Query: 361 HIVNIEALKSSPLSLERPLDLFIGVFSTANNFKYRMAVRRTWIQYPEVRSGSVAVRFFVG 420
           HIVNIEALKSSPLS  +PLDLFIGVFSTANNFKYRMA+RRTW+QYP V+SGSVAVRFFVG
Sbjct: 361 HIVNIEALKSSPLSPAKPLDLFIGVFSTANNFKYRMAIRRTWMQYPAVQSGSVAVRFFVG 420

Query: 421 LHKNQIVNEELWDEARTYGDIQMMPFVDYYSLITWKTLGICIFGAEIASAKYIMKTDDDA 480
           LHKNQIVNEELWDEARTYGDIQMMPFVDYYS+ITWKTLGICIFGAEIASAKYIMKTDDDA
Sbjct: 421 LHKNQIVNEELWDEARTYGDIQMMPFVDYYSVITWKTLGICIFGAEIASAKYIMKTDDDA 480

Query: 481 FVRVDEVLASLKRIDVQSGLLYGLINSDSQPHRDPESKWYISMEEWPEDNYPTWAHGPGY 540
           FVRVDEVLASLKRID QSGLLYGLINSDSQPHRDPESKWYISME WPEDNYPTWAHGPGY
Sbjct: 481 FVRVDEVLASLKRIDAQSGLLYGLINSDSQPHRDPESKWYISMEAWPEDNYPTWAHGPGY 540

Query: 541 VVSNDIAKTISKKYKEGRLKMFKLEDVAMGIWIENMKREGLKIRYEKDDRIHIEGCKDDY 600
           VVSNDIAKTISK YKEGRLKMFKLEDVAMGIWIENMK++GLK++YEKDDRIHIEGCKDDY
Sbjct: 541 VVSNDIAKTISKNYKEGRLKMFKLEDVAMGIWIENMKKDGLKVQYEKDDRIHIEGCKDDY 600

Query: 601 MVAHYQGPREMLCLWQKLQEGNGIRCCGSDN 632
           +VAHYQGPREMLCLWQKLQEGNGIRCCGSDN
Sbjct: 601 VVAHYQGPREMLCLWQKLQEGNGIRCCGSDN 631

BLAST of Tan0009873 vs. ExPASy TrEMBL
Match: A0A6J1DBD8 (beta-1,3-galactosyltransferase GALT1 OS=Momordica charantia OX=3673 GN=LOC111019141 PE=3 SV=1)

HSP 1 Score: 1214.5 bits (3141), Expect = 0.0e+00
Identity = 584/631 (92.55%), Postives = 612/631 (96.99%), Query Frame = 0

Query: 1   MKKSYGRVLLASLFMLVLMVYVILRSSTRQDYYATPLYTSVDPLQWLNPAVPPAVQNPEI 60
           MKKSYGRVL+ASLF L+LM+YVI RSS+R++YYA PLY S+DPLQWLNPAVPPAVQNPEI
Sbjct: 1   MKKSYGRVLVASLFTLLLMIYVITRSSSRENYYANPLYNSIDPLQWLNPAVPPAVQNPEI 60

Query: 61  GHQVISADSIISSLFTQGNFTDGENRALQTWNHLRSIINCTRGLPNAVEAIKEAGGVWDV 120
            +QVISADSI+SSLFTQGNFTDGEN++LQTWNHLRSIINCTRGLPNAVEAIKEAGGVWD 
Sbjct: 61  AYQVISADSIVSSLFTQGNFTDGENQSLQTWNHLRSIINCTRGLPNAVEAIKEAGGVWDF 120

Query: 121 LKTSIEKERLGYTNESARAKEKQCPHFLTKMNATKLDNNGHKLRMPCGLTQGSSITVIGI 180
           LKTSIEKERLGYTNESARAKEKQCPHFLTKMNATK+D+NGHKLRMPCGLTQGSSITVIGI
Sbjct: 121 LKTSIEKERLGYTNESARAKEKQCPHFLTKMNATKVDSNGHKLRMPCGLTQGSSITVIGI 180

Query: 181 PDGLLGNFRIDLTGEPLPGEPDPPLILHYNVRLLGDKLTEDPVIVQNTWTVSRDWGDEER 240
           PDGLLGNFRIDLTGEPLPGEP+PP+ILHYNVRLLGDKLTEDPVIVQNTWTVSRDWG+E+R
Sbjct: 181 PDGLLGNFRIDLTGEPLPGEPEPPIILHYNVRLLGDKLTEDPVIVQNTWTVSRDWGEEDR 240

Query: 241 CPSPGSDENGKVDELEKCNKIVGNVETRLSESNKNFNKSKSMVQGAKTRPYFPFKLGQPF 300
           CP PGSDENGKVDELEKCNKIVGNVE RL E NKNFNKSKS+VQGAKT  YFPFKLG PF
Sbjct: 241 CPPPGSDENGKVDELEKCNKIVGNVENRLPELNKNFNKSKSVVQGAKTIKYFPFKLGHPF 300

Query: 301 AATLRVGVDGIQMTVDGKHVTSFAYRETLEPWLVSEVKISGDLKLISVLASGLPTSEDSD 360
           AATLRVGVDGIQMTVDGKHVTSFAYRETLEPWLVSEVKISGDLKLISVLASGLPTSEDSD
Sbjct: 301 AATLRVGVDGIQMTVDGKHVTSFAYRETLEPWLVSEVKISGDLKLISVLASGLPTSEDSD 360

Query: 361 HIVNIEALKSSPLSLERPLDLFIGVFSTANNFKYRMAVRRTWIQYPEVRSGSVAVRFFVG 420
           HIVNIEALKS+PLS E+ LDLFIGVFSTANNFKYRMAVRRTW+QY  V+SGSVAVRFFVG
Sbjct: 361 HIVNIEALKSTPLSTEKQLDLFIGVFSTANNFKYRMAVRRTWMQYLAVQSGSVAVRFFVG 420

Query: 421 LHKNQIVNEELWDEARTYGDIQMMPFVDYYSLITWKTLGICIFGAEIASAKYIMKTDDDA 480
           LHKNQIVNEELWDEARTYGDIQMMPFVDYYSLITWKTLGICIFGAEIASAKYIMKTDDDA
Sbjct: 421 LHKNQIVNEELWDEARTYGDIQMMPFVDYYSLITWKTLGICIFGAEIASAKYIMKTDDDA 480

Query: 481 FVRVDEVLASLKRIDVQSGLLYGLINSDSQPHRDPESKWYISMEEWPEDNYPTWAHGPGY 540
           FVRVDEVLASLKRI+ QSGLLYGLINSDSQPHR+PESKWYISMEEWPE+NYPTWAHGPGY
Sbjct: 481 FVRVDEVLASLKRINAQSGLLYGLINSDSQPHRNPESKWYISMEEWPEENYPTWAHGPGY 540

Query: 541 VVSNDIAKTISKKYKEGRLKMFKLEDVAMGIWIENMKREGLKIRYEKDDRIHIEGCKDDY 600
           VVS+DIAKTISKKYKEGRLKMFKLEDVAMGIWIENMKREGL+IRYEKDDRIHIEGCKDDY
Sbjct: 541 VVSSDIAKTISKKYKEGRLKMFKLEDVAMGIWIENMKREGLQIRYEKDDRIHIEGCKDDY 600

Query: 601 MVAHYQGPREMLCLWQKLQEGNGIRCCGSDN 632
           +VAHYQ PREMLCLWQKLQEGNGIRCCGSDN
Sbjct: 601 VVAHYQEPREMLCLWQKLQEGNGIRCCGSDN 631

BLAST of Tan0009873 vs. ExPASy TrEMBL
Match: A0A1S3BFK4 (beta-1,3-galactosyltransferase GALT1 OS=Cucumis melo OX=3656 GN=LOC103489127 PE=3 SV=1)

HSP 1 Score: 1190.6 bits (3079), Expect = 0.0e+00
Identity = 576/630 (91.43%), Postives = 606/630 (96.19%), Query Frame = 0

Query: 1   MKKSYGRVLLASLFMLVLMVYVILRSSTRQDYYATPLYTSVDPLQWLNPAVPPAVQNPEI 60
           MKKSYGRVL+ASL ML+LM+YVI RSSTRQ+YYA P Y S+DPLQWLNPAVPPAV NPE 
Sbjct: 1   MKKSYGRVLVASLSMLLLMIYVISRSSTRQNYYAAPFYNSMDPLQWLNPAVPPAVLNPEN 60

Query: 61  GHQVISADSIISSLFTQGNFTDGENRALQTWNHLRSIINCTRGLPNAVEAIKEAGGVWDV 120
            +QVISADSIISSLFT GNFTDGEN++LQTWNHLRSIIN TRGLPN+VEAIKEA GVW +
Sbjct: 61  AYQVISADSIISSLFTPGNFTDGENQSLQTWNHLRSIINYTRGLPNSVEAIKEARGVWSI 120

Query: 121 LKTSIEKERLGYTNESARAKEKQCPHFLTKMNATKLDNNGHKLRMPCGLTQGSSITVIGI 180
           LKT+IE ERLG TNE+ARAKEKQCPHFLTKMNATKLDN GHKLRMPCGLTQGSSITVIGI
Sbjct: 121 LKTTIENERLGSTNETARAKEKQCPHFLTKMNATKLDNIGHKLRMPCGLTQGSSITVIGI 180

Query: 181 PDGLLGNFRIDLTGEPLPGEPDPPLILHYNVRLLGDKLTEDPVIVQNTWTVSRDWGDEER 240
           PDGLLGNFRIDLTGEPLPGEPDPP+ILHYNVRLLGDKLTEDPVIVQNTWTVSRDWG+EER
Sbjct: 181 PDGLLGNFRIDLTGEPLPGEPDPPIILHYNVRLLGDKLTEDPVIVQNTWTVSRDWGEEER 240

Query: 241 CPSPGSDENGKVDELEKCNKIVGNVETRLSESNKNFNKSKSMVQGAKTRPYFPFKLGQPF 300
           CPS GSDENGKVDELEKCNKIVGN+ETRLSE NKNFNKSKSMVQGAKTR YFPFKLG PF
Sbjct: 241 CPS-GSDENGKVDELEKCNKIVGNIETRLSELNKNFNKSKSMVQGAKTRAYFPFKLGHPF 300

Query: 301 AATLRVGVDGIQMTVDGKHVTSFAYRETLEPWLVSEVKISGDLKLISVLASGLPTSEDSD 360
           AATLRVGVDGIQMTVDGKHVTSFAYRETLEPWLVSEVKISGDLKLISVLASGLPTSEDSD
Sbjct: 301 AATLRVGVDGIQMTVDGKHVTSFAYRETLEPWLVSEVKISGDLKLISVLASGLPTSEDSD 360

Query: 361 HIVNIEALKSSPLSLERPLDLFIGVFSTANNFKYRMAVRRTWIQYPEVRSGSVAVRFFVG 420
           HIVNIEALKS+PLS +R L+LFIGVFSTANNFKYRMAVRRTW+QYPEV++GSVAVRFFVG
Sbjct: 361 HIVNIEALKSTPLSPDRTLELFIGVFSTANNFKYRMAVRRTWMQYPEVQAGSVAVRFFVG 420

Query: 421 LHKNQIVNEELWDEARTYGDIQMMPFVDYYSLITWKTLGICIFGAEIASAKYIMKTDDDA 480
           LHKNQIVNEELWDEARTYGDIQMMPFVDYYSLITWKTLGICIFGAEIASAKYIMKTDDDA
Sbjct: 421 LHKNQIVNEELWDEARTYGDIQMMPFVDYYSLITWKTLGICIFGAEIASAKYIMKTDDDA 480

Query: 481 FVRVDEVLASLKRIDVQSGLLYGLINSDSQPHRDPESKWYISMEEWPEDNYPTWAHGPGY 540
           FVRVDEVLASLKRI+ QSGLLYGLINSDSQPHRDPESKWYIS+EEWPEDNYPTWAHGPGY
Sbjct: 481 FVRVDEVLASLKRINAQSGLLYGLINSDSQPHRDPESKWYISLEEWPEDNYPTWAHGPGY 540

Query: 541 VVSNDIAKTISKKYKEGRLKMFKLEDVAMGIWIENMKREGLKIRYEKDDRIHIEGCKDDY 600
           VVS+DIAKTISKKYKEG LKMFKLEDVAMGIWI N+K+EGL+IRYEKD+RIHIEGCKDDY
Sbjct: 541 VVSSDIAKTISKKYKEGSLKMFKLEDVAMGIWIANIKKEGLEIRYEKDERIHIEGCKDDY 600

Query: 601 MVAHYQGPREMLCLWQKLQEGNGIRCCGSD 631
           +VAHYQGPREMLCLWQKLQEGNG+RCCGSD
Sbjct: 601 VVAHYQGPREMLCLWQKLQEGNGVRCCGSD 629

BLAST of Tan0009873 vs. ExPASy TrEMBL
Match: A0A6J1JAC3 (beta-1,3-galactosyltransferase GALT1-like OS=Cucurbita maxima OX=3661 GN=LOC111484973 PE=3 SV=1)

HSP 1 Score: 1169.1 bits (3023), Expect = 0.0e+00
Identity = 568/631 (90.02%), Postives = 595/631 (94.29%), Query Frame = 0

Query: 1   MKKSYGRVLLASLFMLVLMVYVILRSSTRQDYYATPLYTSVDPLQWLNPAVPPAVQNPEI 60
           MKKSYGRVL+AS  ML+LMVYVI RSSTRQ+YYAT  + SVDPLQWLNPAVP AVQNPEI
Sbjct: 1   MKKSYGRVLVASFCMLLLMVYVISRSSTRQNYYATRSFNSVDPLQWLNPAVPSAVQNPEI 60

Query: 61  GHQVISADSIISSLFTQGNFTDGENRALQTWNHLRSIINCTRGLPNAVEAIKEAGGVWDV 120
            +QVI+ADSIISSLFTQGNFT+ E + LQTWNHLRSI N TRGLPNAVEAIKEAGGVW+ 
Sbjct: 61  AYQVITADSIISSLFTQGNFTNEEKQYLQTWNHLRSITNYTRGLPNAVEAIKEAGGVWNN 120

Query: 121 LKTSIEKERLGYTNESARAKEKQCPHFLTKMNATKLDNNGHKLRMPCGLTQGSSITVIGI 180
           LKTSIEKERLG TNESARAKEKQCPHFLTKMNATKLDNNGH LRMPCGLTQGSSITVIGI
Sbjct: 121 LKTSIEKERLGSTNESARAKEKQCPHFLTKMNATKLDNNGHNLRMPCGLTQGSSITVIGI 180

Query: 181 PDGLLGNFRIDLTGEPLPGEPDPPLILHYNVRLLGDKLTEDPVIVQNTWTVSRDWGDEER 240
           PDGLLGNF+IDLTGEPLPGEPDP +ILHYNVRLLGDKLTEDPVIVQNTWTVS+DWG+EER
Sbjct: 181 PDGLLGNFQIDLTGEPLPGEPDPSIILHYNVRLLGDKLTEDPVIVQNTWTVSQDWGEEER 240

Query: 241 CPSPGSDENGKVDELEKCNKIVGNVETRLSESNKNFNKSKSMVQGAKTRPYFPFKLGQPF 300
           CPS  SD+NGKVDELEKCNKIVGNVETRLSE NKNFNKSK  VQGAKT+PYFPFKLG PF
Sbjct: 241 CPSSVSDDNGKVDELEKCNKIVGNVETRLSELNKNFNKSKPTVQGAKTKPYFPFKLGHPF 300

Query: 301 AATLRVGVDGIQMTVDGKHVTSFAYRETLEPWLVSEVKISGDLKLISVLASGLPTSEDSD 360
           AATLRVGVDGIQ TVDGKHVTSFAYRETLEPWLVSEVKISGDLKLISVLASGLPTSEDSD
Sbjct: 301 AATLRVGVDGIQTTVDGKHVTSFAYRETLEPWLVSEVKISGDLKLISVLASGLPTSEDSD 360

Query: 361 HIVNIEALKSSPLSLERPLDLFIGVFSTANNFKYRMAVRRTWIQYPEVRSGSVAVRFFVG 420
           HIVNIEALKS PLS +RPLDLFIGVFSTANNFKYRMAVRRTW+QYPEVRSGSVAVRFFVG
Sbjct: 361 HIVNIEALKSIPLSPDRPLDLFIGVFSTANNFKYRMAVRRTWMQYPEVRSGSVAVRFFVG 420

Query: 421 LHKNQIVNEELWDEARTYGDIQMMPFVDYYSLITWKTLGICIFGAEIASAKYIMKTDDDA 480
           LHKNQIVNEE+WDEARTYGDIQMMPFVDYYSLITWKTLGICIFGAEIAS+KYIMKTDDDA
Sbjct: 421 LHKNQIVNEEVWDEARTYGDIQMMPFVDYYSLITWKTLGICIFGAEIASSKYIMKTDDDA 480

Query: 481 FVRVDEVLASLKRIDVQSGLLYGLINSDSQPHRDPESKWYISMEEWPEDNYPTWAHGPGY 540
           FVRVDEVLASL RI  QSGLLYGLINSDSQPHRDP+SKWYI  EEWPE+NYPTWAHGPGY
Sbjct: 481 FVRVDEVLASLNRISAQSGLLYGLINSDSQPHRDPDSKWYIGTEEWPEENYPTWAHGPGY 540

Query: 541 VVSNDIAKTISKKYKEGRLKMFKLEDVAMGIWIENMKREGLKIRYEKDDRIHIEGCKDDY 600
           VVS+DIAKTISK YKEG LKMFKLEDVAMGIWIENMKREGL IRYEKD+RIHIEGCK +Y
Sbjct: 541 VVSSDIAKTISKMYKEGSLKMFKLEDVAMGIWIENMKREGLDIRYEKDERIHIEGCKYNY 600

Query: 601 MVAHYQGPREMLCLWQKLQEGNGIRCCGSDN 632
           +VAHYQGP+EMLCLWQKLQEGNG+RCCG DN
Sbjct: 601 VVAHYQGPQEMLCLWQKLQEGNGVRCCGPDN 631

BLAST of Tan0009873 vs. TAIR 10
Match: AT1G26810.1 (galactosyltransferase1 )

HSP 1 Score: 783.9 bits (2023), Expect = 9.8e-227
Identity = 385/640 (60.16%), Postives = 480/640 (75.00%), Query Frame = 0

Query: 1   MKKSYGRVLLASLFM-LVLMVYVILRSSTRQDYYATPLYTSVD-----PLQWLNPAVPPA 60
           MK+ YG +L+ S+ M L +  YV L +   + Y        V      P++WL   +P  
Sbjct: 1   MKRFYGGLLVVSMCMFLTVYRYVDLNTPVEKPYITAAASVVVTPNTTLPMEWLRITLPDF 60

Query: 61  VQNPEIGHQVISAD--SIISSLFTQGNFTDGENRALQTWNHLRSIINCTRGLPNAVEAIK 120
           ++      + IS D  +++S LF + N +  E   L TWN L S+++  + L N V+AIK
Sbjct: 61  MKEARNTQEAISGDDIAVVSGLFVEQNVSKEEREPLLTWNRLESLVDNAQSLVNGVDAIK 120

Query: 121 EAGGVWDVLKTSIEKERLGYTNE--SARAKEKQCPHFLTKMNATKLDNNGHKLRMPCGLT 180
           EAG VW+ L +++E ++L   NE  + + KE+ CP FL+KMNAT+ D +  KL++PCGLT
Sbjct: 121 EAGIVWESLVSAVEAKKLVDVNENQTRKGKEELCPQFLSKMNATEADGSSLKLQIPCGLT 180

Query: 181 QGSSITVIGIPDGLLGNFRIDLTGEPLPGEPDPPLILHYNVRLLGDKLTEDPVIVQNTWT 240
           QGSSITVIGIPDGL+G+FRIDLTG+PLPGEPDPP+I+HYNVRLLGDK TEDPVIVQN+WT
Sbjct: 181 QGSSITVIGIPDGLVGSFRIDLTGQPLPGEPDPPIIVHYNVRLLGDKSTEDPVIVQNSWT 240

Query: 241 VSRDWGDEERCPSPGSDENGKVDELEKCNKIVGNVETRLSESNKNFNKSKSMV---QGAK 300
            S+DWG EERCP    D N KVD+L++CNK+VG    R S ++   N S+ +    + +K
Sbjct: 241 ASQDWGAEERCPKFDPDMNKKVDDLDECNKMVGGEINRTSSTSLQSNTSRGVPVAREASK 300

Query: 301 TRPYFPFKLGQPFAATLRVGVDGIQMTVDGKHVTSFAYRETLEPWLVSEVKISGDLKLIS 360
              YFPFK G    ATLRVG +G+QMTVDGKH+TSFA+R+TLEPWLVSE++I+GD +LIS
Sbjct: 301 HEKYFPFKQGFLSVATLRVGTEGMQMTVDGKHITSFAFRDTLEPWLVSEIRITGDFRLIS 360

Query: 361 VLASGLPTSEDSDHIVNIEALKSSPLSLERPLDLFIGVFSTANNFKYRMAVRRTWIQYPE 420
           +LASGLPTSE+S+H+V++EALKS  LS  RPLDL IGVFSTANNFK RMAVRRTW+QY +
Sbjct: 361 ILASGLPTSEESEHVVDLEALKSPTLSPLRPLDLVIGVFSTANNFKRRMAVRRTWMQYDD 420

Query: 421 VRSGSVAVRFFVGLHKNQIVNEELWDEARTYGDIQMMPFVDYYSLITWKTLGICIFGAEI 480
           VRSG VAVRFFVGLHK+ +VN ELW+EARTYGD+Q+MPFVDYYSLI+WKTL ICIFG E+
Sbjct: 421 VRSGRVAVRFFVGLHKSPLVNLELWNEARTYGDVQLMPFVDYYSLISWKTLAICIFGTEV 480

Query: 481 ASAKYIMKTDDDAFVRVDEVLASLKRIDVQSGLLYGLINSDSQPHRDPESKWYISMEEWP 540
            SAK+IMKTDDDAFVRVDEVL SL   +   GL+YGLINSDSQP R+P+SKWYIS EEWP
Sbjct: 481 DSAKFIMKTDDDAFVRVDEVLLSLSMTNNTRGLIYGLINSDSQPIRNPDSKWYISYEEWP 540

Query: 541 EDNYPTWAHGPGYVVSNDIAKTISKKYKEGRLKMFKLEDVAMGIWIENMKREGLKIRYEK 600
           E+ YP WAHGPGY+VS DIA+++ K +KEG LKMFKLEDVAMGIWI  + + GL+  YE 
Sbjct: 541 EEKYPPWAHGPGYIVSRDIAESVGKLFKEGNLKMFKLEDVAMGIWIAELTKHGLEPHYEN 600

Query: 601 DDRIHIEGCKDDYMVAHYQGPREMLCLWQKLQEGNGIRCC 628
           D RI  +GCKD Y+VAHYQ P EM CLW+K QE     CC
Sbjct: 601 DGRIISDGCKDGYVVAHYQSPAEMTCLWRKYQETKRSLCC 640

BLAST of Tan0009873 vs. TAIR 10
Match: AT3G06440.1 (Galactosyltransferase family protein )

HSP 1 Score: 513.5 bits (1321), Expect = 2.5e-145
Identity = 263/557 (47.22%), Postives = 356/557 (63.91%), Query Frame = 0

Query: 74  LFTQGNFTDGE--NRALQTWNHLRSIINCTRGLPNAVEAIKEAGGVWDVLKTSIEKERLG 133
           LF+  +F   E  +  +  W+ +R  +     LP   + I+EA      L   I +E+  
Sbjct: 76  LFSNKSFFGEEEVSNGMLVWSRMRPFLERPDALPETAQGIEEATLAMKGLVLEINREKRA 135

Query: 134 YTNESARAKEKQ-CPHFLTKMNATKLDNNGHKLRMPCGLTQGSSITVIGIPDGLLGNFRI 193
           Y++     + ++ CP F+T  +      +   L +PCGL + SSIT++GIPD    +F+I
Sbjct: 136 YSSGMVSKEIRRICPDFVTAFDKDLSGLSHVLLELPCGLIEDSSITLVGIPDEHSSSFQI 195

Query: 194 DLTGEPLPGEPDPPLILHYNVRLLGDKLTEDPVIVQNTWTVSRDWGDEERCPSPGSDENG 253
            L G  L GE   P+IL YNV          P IVQNTWT    WG+EERC   GS +N 
Sbjct: 196 QLVGSGLSGETRRPIILRYNVNF------SKPSIVQNTWTEKLGWGNEERCQYHGSLKNH 255

Query: 254 KVDELEKCNKIVGNVETRLSESNKNFNKSKSMVQGAKTRPYFPFKLGQPFAATLRVGVDG 313
            VDEL  CNK  G +   +SE + N + +  +   + +   FPF  G PF A L  G++G
Sbjct: 256 LVDELPLCNKQTGRI---ISEKSSNDDATMEL---SLSNANFPFLKGSPFTAALWFGLEG 315

Query: 314 IQMTVDGKHVTSFAYRETLEPWLVSEVKISGDLKLISVLASGLPTSEDSDHIVNIEALKS 373
             MT++G+H TSFAYRE LEPWLVS VK+SG LK++SVLA+ LP  +D   ++  E LK+
Sbjct: 316 FHMTINGRHETSFAYREKLEPWLVSAVKVSGGLKILSVLATRLPIPDDHASLIIEEKLKA 375

Query: 374 SPLSLERPLDLFIGVFSTANNFKYRMAVRRTWIQYPEVRSGSVAVRFFVGLHKNQIVNEE 433
             LS  R ++L +GVFST NNFK RMA+RR+W+QY  VRSG VAVRF +GLH N+ VN E
Sbjct: 376 PSLSGTR-IELLVGVFSTGNNFKRRMALRRSWMQYEAVRSGKVAVRFLIGLHTNEKVNLE 435

Query: 434 LWDEARTYGDIQMMPFVDYYSLITWKTLGICIFGAEIASAKYIMKTDDDAFVRVDEVLAS 493
           +W E++ YGDIQ MPFVDYY L++ KT+ +CI G ++  AKYIMKTDDDAFVR+DE+L+S
Sbjct: 436 MWRESKAYGDIQFMPFVDYYGLLSLKTVALCILGTKVIPAKYIMKTDDDAFVRIDELLSS 495

Query: 494 LKRIDVQSGLLYGLINSDSQPHRDPESKWYISMEEWPEDNYPTWAHGPGYVVSNDIAKTI 553
           L+     S LLYGLI+ DS P R+  SKW+I  EEWP D+YP WAHGPGY++S+DIAK +
Sbjct: 496 LEE-RPSSALLYGLISFDSSPDREQGSKWFIPKEEWPLDSYPPWAHGPGYIISHDIAKFV 555

Query: 554 SKKYKEGRLKMFKLEDVAMGIWIENMKREGLKIRYEKDDRIHIEGCKDDYMVAHYQGPRE 613
            K +++  L +FKLEDVAMGIWI+   +   +++Y  D R H   CK +Y++ HYQ PR 
Sbjct: 556 VKGHRQRDLGLFKLEDVAMGIWIQQFNQTIKRVKYINDKRFHNSDCKSNYILVHYQTPRL 615

Query: 614 MLCLWQKLQEGNGIRCC 628
           +LCLW+KLQ+ N   CC
Sbjct: 616 ILCLWEKLQKENQSICC 618

BLAST of Tan0009873 vs. TAIR 10
Match: AT3G06440.2 (Galactosyltransferase family protein )

HSP 1 Score: 436.0 bits (1120), Expect = 5.0e-122
Identity = 228/486 (46.91%), Postives = 309/486 (63.58%), Query Frame = 0

Query: 74  LFTQGNFTDGE--NRALQTWNHLRSIINCTRGLPNAVEAIKEAGGVWDVLKTSIEKERLG 133
           LF+  +F   E  +  +  W+ +R  +     LP   + I+EA      L   I +E+  
Sbjct: 76  LFSNKSFFGEEEVSNGMLVWSRMRPFLERPDALPETAQGIEEATLAMKGLVLEINREKRA 135

Query: 134 YTNESARAKEKQ-CPHFLTKMNATKLDNNGHKLRMPCGLTQGSSITVIGIPDGLLGNFRI 193
           Y++     + ++ CP F+T  +      +   L +PCGL + SSIT++GIPD    +F+I
Sbjct: 136 YSSGMVSKEIRRICPDFVTAFDKDLSGLSHVLLELPCGLIEDSSITLVGIPDEHSSSFQI 195

Query: 194 DLTGEPLPGEPDPPLILHYNVRLLGDKLTEDPVIVQNTWTVSRDWGDEERCPSPGSDENG 253
            L G  L GE   P+IL YNV          P IVQNTWT    WG+EERC   GS +N 
Sbjct: 196 QLVGSGLSGETRRPIILRYNVNF------SKPSIVQNTWTEKLGWGNEERCQYHGSLKNH 255

Query: 254 KVDELEKCNKIVGNVETRLSESNKNFNKSKSMVQGAKTRPYFPFKLGQPFAATLRVGVDG 313
            VDEL  CNK  G +   +SE + N + +  +   + +   FPF  G PF A L  G++G
Sbjct: 256 LVDELPLCNKQTGRI---ISEKSSNDDATMEL---SLSNANFPFLKGSPFTAALWFGLEG 315

Query: 314 IQMTVDGKHVTSFAYRETLEPWLVSEVKISGDLKLISVLASGLPTSEDSDHIVNIEALKS 373
             MT++G+H TSFAYRE LEPWLVS VK+SG LK++SVLA+ LP  +D   ++  E LK+
Sbjct: 316 FHMTINGRHETSFAYREKLEPWLVSAVKVSGGLKILSVLATRLPIPDDHASLIIEEKLKA 375

Query: 374 SPLSLERPLDLFIGVFSTANNFKYRMAVRRTWIQYPEVRSGSVAVRFFVGLHKNQIVNEE 433
             LS  R ++L +GVFST NNFK RMA+RR+W+QY  VRSG VAVRF +GLH N+ VN E
Sbjct: 376 PSLSGTR-IELLVGVFSTGNNFKRRMALRRSWMQYEAVRSGKVAVRFLIGLHTNEKVNLE 435

Query: 434 LWDEARTYGDIQMMPFVDYYSLITWKTLGICIFGAEIASAKYIMKTDDDAFVRVDEVLAS 493
           +W E++ YGDIQ MPFVDYY L++ KT+ +CI G ++  AKYIMKTDDDAFVR+DE+L+S
Sbjct: 436 MWRESKAYGDIQFMPFVDYYGLLSLKTVALCILGTKVIPAKYIMKTDDDAFVRIDELLSS 495

Query: 494 LKRIDVQSGLLYGLINSDSQPHRDPESKWYISMEEWPEDNYPTWAHGPGYVVSNDIAKTI 553
           L+     S LLYGLI+ DS P R+  SKW+I  EEWP D+YP WAHGPGY++S+DIAK +
Sbjct: 496 LEE-RPSSALLYGLISFDSSPDREQGSKWFIPKEEWPLDSYPPWAHGPGYIISHDIAKFV 547

Query: 554 SKKYKE 557
            K +++
Sbjct: 556 VKGHRQ 547

BLAST of Tan0009873 vs. TAIR 10
Match: AT1G74800.1 (Galactosyltransferase family protein )

HSP 1 Score: 347.1 bits (889), Expect = 3.1e-95
Identity = 199/542 (36.72%), Postives = 295/542 (54.43%), Query Frame = 0

Query: 107 AVEAIKEAGGVWDV---LKTSIEKERL-GYTNESARAKEKQCPHFLTKMNATKLDNNGHK 166
           +VE  K A   W +   L   +E  RL     +  + K   CPH ++   +  ++     
Sbjct: 133 SVELHKSAKEAWQLGRKLWKELESGRLEKLVEKPEKNKPDSCPHSVSLTGSEFMNRENKL 192

Query: 167 LRMPCGLTQGSSITVIGIP----------DGLLGNFRIDLTGEPLPGEPDPPLILHYNVR 226
           + +PCGLT GS IT++G P            L+  F I+L G       DPP ILH+N R
Sbjct: 193 MELPCGLTLGSHITLVGRPRKAHPKEGDWSKLVSQFVIELQGLKTVEGEDPPRILHFNPR 252

Query: 227 LLGDKLTEDPVIVQNTWTVSRDWGDEERCPS-PGSDENGKVDELEKCNKIVGNVETRLSE 286
           L GD  ++ PVI QN+      WG  +RC      D+   VD   KC K + + +   SE
Sbjct: 253 LKGD-WSKKPVIEQNS-CYRMQWGPAQRCEGWKSRDDEETVDSHVKCEKWIRD-DDNYSE 312

Query: 287 SNKN---FNKSKSMVQGAKTRPYFPFKLGQPFAATLRVGVDGIQMTVDGKHVTSFAYRET 346
            ++     N+     +  K    FPF   + F  TL  G++G  + VDGKHVTSF YR  
Sbjct: 313 GSRARWWLNRLIGRRKRVKVEWPFPFVEEKLFVLTLSAGLEGYHINVDGKHVTSFPYRTG 372

Query: 347 LEPWLVSEVKISGDLKLISVLASGLPTSEDS---DHIVNIEALKSSPLSLERPLDLFIGV 406
                 + + ++GD+ + SV  + LPTS  S      + +     +P+  + P+++FIG+
Sbjct: 373 FTLEDATGLTVNGDIDVHSVFVASLPTSHPSFAPQRHLELSKRWQAPVVPDGPVEIFIGI 432

Query: 407 FSTANNFKYRMAVRRTWIQYPEVRSGSVAVRFFVGLHKNQIVNEELWDEARTYGDIQMMP 466
            S  N+F  RMAVR++W+Q+  + S  V  RFFV LH  + VN EL  EA  +GDI ++P
Sbjct: 433 LSAGNHFSERMAVRKSWMQHVLITSAKVVARFFVALHGRKEVNVELKKEAEYFGDIVLVP 492

Query: 467 FVDYYSLITWKTLGICIFGAEIASAKYIMKTDDDAFVRVDEVLASLKRIDVQSGLLYGLI 526
           ++D Y L+  KT+ IC  GA   SAKYIMK DDD FV++  V+  +K++     L  G +
Sbjct: 493 YMDSYDLVVLKTVAICEHGALAFSAKYIMKCDDDTFVKLGAVINEVKKVPEGRSLYIGNM 552

Query: 527 NSDSQPHRDPESKWYISMEEWPEDNYPTWAHGPGYVVSNDIAKTISKKYKEGRLKMFKLE 586
           N   +P R    KW ++ EEWPE++YP +A+GPGYV+S+DIA+ I  K++  +L++FK+E
Sbjct: 553 NYYHKPLRG--GKWAVTYEEWPEEDYPPYANGPGYVLSSDIARFIVDKFERHKLRLFKME 612

Query: 587 DVAMGIWIENMKREGLKIRYEKDDRIHIEGCKDDYMVAHYQGPREMLCLWQKLQEGNGIR 628
           DV++G+W+E+ K     + Y    R    GC ++Y  AHYQ PR+M+CLW KL   N   
Sbjct: 613 DVSVGMWVEHFKNTTNPVDYRHSLRFCQFGCVENYYTAHYQSPRQMICLWDKLLRQNKPE 669

BLAST of Tan0009873 vs. TAIR 10
Match: AT5G62620.1 (Galactosyltransferase family protein )

HSP 1 Score: 345.1 bits (884), Expect = 1.2e-94
Identity = 203/558 (36.38%), Postives = 302/558 (54.12%), Query Frame = 0

Query: 107 AVEAIKEAGGVWDVLKTSIEKERLGYTNESARAKEKQ--------CPHFLTKMNATKLDN 166
           +VE  K A   W+V +   E+   G T ++   ++K+               +  + L  
Sbjct: 125 SVELHKSAKVAWEVGRKIWEELESGKTLKALEKEKKKKIEEHGTNSCSLSVSLTGSDLLK 184

Query: 167 NGHKLRMPCGLTQGSSITVIGIPDGL-------------------LGNFRIDLTGEPLPG 226
            G+ + +PCGLT GS ITV+G P                      +  F+++L G     
Sbjct: 185 RGNIMELPCGLTLGSHITVVGKPRAAHSEKDPKISMLKEGDEAVKVSQFKLELQGLKAVE 244

Query: 227 EPDPPLILHYNVRLLGDKLTEDPVIVQNTWTVSRDWGDEERCPSPGS-DENGKVDELEKC 286
             +PP ILH N RL GD  +  PVI QNT      WG  +RC    S D+   VD   KC
Sbjct: 245 GEEPPRILHLNPRLKGD-WSGKPVIEQNT-CYRMQWGSAQRCEGWRSRDDEETVDGQVKC 304

Query: 287 NKIV--GNVETRLSESNKNFNKSKSMVQGAKTRPY----FPFKLGQPFAATLRVGVDGIQ 346
            K     ++ ++  ES+K  +   S + G   +      FPF + + F  TL  G++G  
Sbjct: 305 EKWARDDSITSKEEESSKAASWWLSRLIGRSKKVTVEWPFPFTVDKLFVLTLSAGLEGYH 364

Query: 347 MTVDGKHVTSFAYRETLEPWLVSEVKISGDLKLISVLASGLPTSEDS---DHIVNIEALK 406
           ++VDGKHVTSF YR        + + I+GD+ + SV A  LPTS  S      + + +  
Sbjct: 365 VSVDGKHVTSFPYRTGFTLEDATGLTINGDIDVHSVFAGSLPTSHPSFSPQRHLELSSNW 424

Query: 407 SSPLSLERPLDLFIGVFSTANNFKYRMAVRRTWIQYPEVRSGSVAVRFFVGLHKNQIVNE 466
            +P   +  +D+FIG+ S  N+F  RMAVRR+W+Q+  V+S  V  RFFV LH  + VN 
Sbjct: 425 QAPSLPDEQVDMFIGILSAGNHFAERMAVRRSWMQHKLVKSSKVVARFFVALHSRKEVNV 484

Query: 467 ELWDEARTYGDIQMMPFVDYYSLITWKTLGICIFGAEIASAKYIMKTDDDAFVRVDEVLA 526
           EL  EA  +GDI ++P++D Y L+  KT+ IC +GA   +AK+IMK DDD FV+VD VL+
Sbjct: 485 ELKKEAEFFGDIVIVPYMDSYDLVVLKTVAICEYGAHQLAAKFIMKCDDDTFVQVDAVLS 544

Query: 527 SLKRIDVQSGLLYGLINSDSQPHRDPESKWYISMEEWPEDNYPTWAHGPGYVVSNDIAKT 586
             K+      L  G IN   +P R  + KW ++ EEWPE++YP +A+GPGY++SNDI++ 
Sbjct: 545 EAKKTPTDRSLYIGNINYYHKPLR--QGKWSVTYEEWPEEDYPPYANGPGYILSNDISRF 604

Query: 587 ISKKYKEGRLKMFKLEDVAMGIWIENMKREGLKIRYEKDDRIHIEGCKDDYMVAHYQGPR 628
           I K++++ +L+MFK+EDV++G+W+E        + Y    R    GC ++Y+ AHYQ PR
Sbjct: 605 IVKEFEKHKLRMFKMEDVSVGMWVEQFNNGTKPVDYIHSLRFCQFGCIENYLTAHYQSPR 664

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8L7F91.4e-22560.16Beta-1,3-galactosyltransferase GALT1 OS=Arabidopsis thaliana OX=3702 GN=GALT1 PE... [more]
Q9ASW13.5e-14447.22Hydroxyproline O-galactosyltransferase GALT3 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q8RX554.3e-9436.72Hydroxyproline O-galactosyltransferase GALT5 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9LV161.6e-9336.38Hydroxyproline O-galactosyltransferase GALT6 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q8GXG62.4e-9235.96Hydroxyproline O-galactosyltransferase GALT4 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Match NameE-valueIdentityDescription
KAG7013139.10.0e+0093.98Beta-1,3-galactosyltransferase GALT1 [Cucurbita argyrosperma subsp. argyrosperma... [more]
XP_022968057.10.0e+0094.29beta-1,3-galactosyltransferase GALT1-like [Cucurbita maxima][more]
KAG6574080.10.0e+0093.82Beta-1,3-galactosyltransferase GALT1, partial [Cucurbita argyrosperma subsp. sor... [more]
XP_022945449.10.0e+0093.66beta-1,3-galactosyltransferase GALT1-like [Cucurbita moschata][more]
XP_023541817.10.0e+0093.82beta-1,3-galactosyltransferase GALT1-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1HSG40.0e+0094.29beta-1,3-galactosyltransferase GALT1-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1G1000.0e+0093.66beta-1,3-galactosyltransferase GALT1-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1DBD80.0e+0092.55beta-1,3-galactosyltransferase GALT1 OS=Momordica charantia OX=3673 GN=LOC111019... [more]
A0A1S3BFK40.0e+0091.43beta-1,3-galactosyltransferase GALT1 OS=Cucumis melo OX=3656 GN=LOC103489127 PE=... [more]
A0A6J1JAC30.0e+0090.02beta-1,3-galactosyltransferase GALT1-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT1G26810.19.8e-22760.16galactosyltransferase1 [more]
AT3G06440.12.5e-14547.22Galactosyltransferase family protein [more]
AT3G06440.25.0e-12246.91Galactosyltransferase family protein [more]
AT1G74800.13.1e-9536.72Galactosyltransferase family protein [more]
AT5G62620.11.2e-9436.38Galactosyltransferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001079Galectin, carbohydrate recognition domainSMARTSM00908Gal_bind_lectin_2coord: 165..350
e-value: 2.9E-29
score: 113.2
IPR001079Galectin, carbohydrate recognition domainSMARTSM00276galectin_3coord: 159..351
e-value: 7.9E-4
score: 22.5
IPR001079Galectin, carbohydrate recognition domainPFAMPF00337Gal-bind_lectincoord: 163..349
e-value: 5.4E-37
score: 126.3
IPR001079Galectin, carbohydrate recognition domainPROSITEPS51304GALECTINcoord: 161..351
score: 38.653545
IPR001079Galectin, carbohydrate recognition domainCDDcd00070GLECTcoord: 160..348
e-value: 8.84327E-26
score: 100.786
NoneNo IPR availableGENE3D3.90.550.50coord: 374..595
e-value: 3.3E-16
score: 61.3
NoneNo IPR availableGENE3D2.60.120.200coord: 158..349
e-value: 2.4E-26
score: 93.8
NoneNo IPR availablePANTHERPTHR11214:SF287SUBFAMILY NOT NAMEDcoord: 64..620
IPR002659Glycosyl transferase, family 31PFAMPF01762Galactosyl_Tcoord: 395..577
e-value: 1.0E-39
score: 136.4
IPR002659Glycosyl transferase, family 31PANTHERPTHR11214BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASEcoord: 64..620
IPR013320Concanavalin A-like lectin/glucanase domain superfamilySUPERFAMILY49899Concanavalin A-like lectins/glucanasescoord: 163..351

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0009873.1Tan0009873.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006486 protein glycosylation
cellular_component GO:0000139 Golgi membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0030246 carbohydrate binding
molecular_function GO:0010488 UDP-galactose:N-glycan beta-1,3-galactosyltransferase activity
molecular_function GO:0016758 hexosyltransferase activity