Tan0008543 (gene) Snake gourd v1

Overview
NameTan0008543
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionGTP-binding protein EngA
LocationLG01: 15194043 .. 15198503 (-)
RNA-Seq ExpressionTan0008543
SyntenyTan0008543
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTCGCTTATGGGTTCCATTGGTCTTCCAACCCCGTGGGTCATCGGCAACGACGTTTTCCATATCGGTAGAACAAGTAGAAAGAACAAGCTCAAATACTCTCTCTCTCAATTTTCTCAGTGGCTCGGTTTTATGCAACGAGGGGCACATGCCTTCAAATTTTTCTACCATTCCCAAGCCCGACACCAAGAAATTCAACCATTCATTCATCAAATTAGGTACCTCCTTCCCGCTACCGTTTCTGTTGATTGCTTGTGTGGGTTTAGTACTTACGATGATGGATTAAAGAACTTTACACCTGCTAGAACTGGGGAGTGATTGCGCATAGTTATACTTGACAATTCGTATTTGTTTTCAAAGACTACTAACGCCGCTTGGTCATTGTTTTTGGGTTACGTAACTCTCTAGGTTGGAATAGTTGTATTTCGGACACAAGATTATCGCTTCATTTCGCTGCTGTTCATATCGCCAGGGGATTTTGTGGAGTATCAGAAAATGGGCACTCGGAGAAATGTTTAAATGATAATGCAAGTGATGGTGATGTTGTTGCTAATAAAGTTCAAGGCGTCCATAGAAACTCTGTGGACTTCACCAAAATTCCTATTAATATGCTTCCAACTGTTGTGCTCGTTGGGCGCCCAAATGTTGGCAAGTCTGCATTATTTAACCGGTAAACTCTTGTATGCTTATATTATTCTAGTAATGAACTTCCCAATGTTGTGCAATGTGAAGTCAGTGATTCTTTTTCTGCATTATCTTGCTGGTATCATGAATTTAGTTCTCTTGGTGTTTTTGTTGTTAATCTATAGGTTTATTAGAAGGAGGGAGGCTCTAGTGTACAACACTCCAGATGATCACGTTACTCGGGATATTCGGGAAGGTGTTGCCAAACTTGGAGATTTGCGGTTTGTAGCATTGGATTAAATGCGCTTAGAGACAACAACATCTTACGCTCTATTCTTGAAAGAACAAAGAAGAATGATCAAAAATGTGCTTCTCAAGTCTCAGCTAGCAATTTTCCTTATTGATGCAAGGTGATTGGTGAATGTTTTTCATAAACTTGGTTGAGATGAATGTAGATATGGATTTAATTGCAAGAGGAATGCCTGTTGTCTTATTGCAATGTATATACATATATACACATGCATCTTTCTTTCGTTTTATCGTGGTTCTGAAGTTTAAAGTAGTCGTGATACTATTTTTCTGAGCAGAGCTGGTCTTCACCCCTTTGATTTGGAGGTTGGGAAGTGGTTTGCATGCAGAGACATGCACCGATATTAACATCGTGGTAGCAATGAATAAATCAATCGTTTTTGACAATGATGTGCACACTTTTTTGGTCTCTTTGCTCTCCGAAGCCCAAAGGTTAGGCTTTGGAGATCCAGTTCCTATATCAGCTGAAACTGGACTTGGCATGCAAGATCTTTATTGGGCCATCAAACCTGTGCTTGAGAAGTATATGTTGAAAGTTATAAATGGTAAGAAATGTACCTATCAATCAATATATCATTCGAAAGATCTTGGCACATTAGCAATAACCACCACAAGTTGATCAAAGAGGCTAATTGTATATCTTGTTTGGATGCCTTTTGAGATTTTGGGTTCAAATTATAATGATCGTCCTTTTGATATTAAATAATATCCTAAGTTTCTTGACACCATTATTGTAGAGTTTGGTAGTTGTGTAATGGAATTAAATCTAGTTTTTGTAAGCTGGTGTATCGACACCTATGGTTATAAGAAAAGAAAAAAATATCAATGTGTTTCTATCCAGCTTTATGAGTGCCCTTCCAGAACATGCATTTTCTTTTATTTTGCATACTTCCTACTTGATTTTGTGGAAGTGCATTCACACGAGAGAGGAGGCCATTATTCATTATTGCAAAGTTTAGCCATCCTTTAATTTGTCTAAAAAATAGCTAGAAACTTGATCGTTCTAGCTTACAGGGATATTAATGTGGCATAGTAAAACATTATAAAACATTATATCTTTTCTGCTATCCTTTGTCAAGACAACTTTTCTCCAAAAACGTACACCATCTTCTTTATAATGGGGTTGGATGTTGGGCAATTATCAACTTTCACCATCGTCCTCTTTGGTCTAACTTGTAGATGATATGGTGATTTTGGCATTATTCTTCCTCCCCACTCTTCTAACCTGGTTCACAATTCCTAGATAATGGTGGTCTTGATCGCTTCCATCTAGTCACAAGCTCCAATGAGGAGGAGGACACCCAGGACAGTAAGGTGCCATTACAGTTAGCAATTGTTGGACGACCTAATGTTGGCAAGTCAACCTTGCTGAACACACTGTTGCAATCGGATCGTGTTTTGGTTGGTCCTGAAGCTGGTTTAACAAGGGATTCAGTTCGAGCTCAATTTGAGTTTGAGGGCAGAACTATATATTTGGTAAGCACGCTGTTTGACTATTTCGTGTTTCTCCTTTTTATGATGCCTTGTACTCTGAATAAAATCTATGTTCCTTAGACGTCCTTTATGTCCTTTATGTTGTGACACTTTCTTTAATGAAGTTCTAATTGTTAATTTTTGGTAATGGTAATGAGTAGGTTGATACAGCTGGTTGGTTGCATAGGACTAAGGAAGAGAAAGGACCAGGATCCTTGAGTGTCATGCAATCGAGTAAGAATCTGATGAGAGCCCATGTGGTTGCTTTGGTTCTTGATGCAGAAGAGGTGTGTGATACAATCAGTTTCTTCCTTATCTTTCTTGTGATTTTTCATGACCATCCACGAACCATTTAATGTTATGGGGTAGCAAGCCTATGCCTAAGTCCACCCCTTGTTTAAGCGTGATTCCAATTTTCTTCTACGGAAAAGTAGAAGATTGTTGGTAGAAGTTGAAAGTTAGTTAAAATTTAAAAGCTTCTTTGAACAAAGTAAAACCTATCACAAGATGATTATTCTTATGGCAACGTGAGACAAATAACTAATTTTTAAGCATTGGACTTTTCTATTGACATATTGATCTATGTGATGTCCAGTTTGCAAGAGAAAGGAGAAGTATGAAACATCTTTGAAGTGGTTATTGCAAGGCGAACGGTGGAGGAAGGTCGTGGTTTGGTAGTAATTGTCAACAAGATGGATCTTATGAGGCGCGACAAAAATTTTGCATCATATGAGAAGATCTTGGAGGTCGCTTCCAGAAGAAATTCAAACAGATAATTCCTCGTATGGTAATTTAGCATTTTATGTGATTTTAGCCGTATCTTACCGGTATAGAAAATGTGATCGAAGAGATTCCTTTTGAAGGAATGAGATTTTCCTATTTTTGGGAGAATATTTCTCAAGTTTGGGGTGAACTCCAAACTCGATTCAATTTCTTTCATTTGGTAAACAACATTGGAAATGAAGATTTTTCATTCCTATTACTCTACTCCTTTCTCACTAAGTAAACATGACCTTAATGTTGGATATTATTATTTCTGACTTCAAAACTTATATAAATTGTGTAGATATTGAAATGGAAATTGGGTTTGTAGGCTAGTTTGATAACCATTTGGTTTTGAAAATTAAGCTTATAAATACTACTTCTATCATGTTTCCTTATCTACTTTTTAGATGTGTTCTAAAAAACCAAGCAAAGTTTTGAAAACTAAAAAAGTAGTTTCCAAAAACTTGTTTTTGTTTTTGAAATTTAGCTAAGAATTTAAATGTTTTCTTAGGAAGGATGGAACCTTTGTAGGGATGATGAAAACCATTGTAGAAAATGTGAGAAAAAGAAATACAATTTTCAAAACCAAAAACCAAATGGTTATCAAACGGGGGCCTTTTTAGAACACCCCTTGCTTTCAGTAAGCAAATGCATATGCTCTCTGTGATTCTTTCTTCTAAATATTAACATGGCAAATGCAAATGGTCATGTACGTGTTAGCGTTAACATGGCTTTTAAATCGGCATCCGTGCAACAGAATACGGTTTATTCATTTCAAAGACTTGAAGGAAGAGGCGGACTTTGCGATCATGCGGCGAGTTATTGAGACATGTGAAAAATGGTGTTCGAGGTTGTCCACAGCTCGTCTTAACCGGTGGTTGCGGAAGGTCAGCAGAAAATACCCTTTTGTTTTCTTAGCTCAGCATATGTATAGCACAATTTGTAAAAGCTCGGGCTTGCACAGGTAATGAGCAGACATTCATGGAAAGATCAATCTGCACAACCAAAGGTCAAGTACTTCACGCAGGTCAAAGCGAGACCGCCTACATTCGTTGCCTTTGTCAATGTGGGAAGACACGACTATCGAATCTGATATCGGTTTTTAACCAAATCGTTGAAGGAAGATTTCGATTTGGGTGGAATTCCTATAAGAATCATGCGCAATGTGTCTTTGCTCCAAAGAAAACAAATTGATGGGGTTGGCAAGAGCAGCAGACACGTTAACCGAACACTTGAAAGAATCCGATCGGATAAGAGACGACCCATCGTTGAAGAACAGACAGCTTGA

mRNA sequence

ATGTCTCGCTTATGGGTTCCATTGGTCTTCCAACCCCGTGGGTCATCGGCAACGACGTTTTCCATATCGGTAGAACAAGTAGAAAGAACAAGCTCAAATACTCTCTCTCTCAATTTTCTCAGTGGCTCGGTTTTATGCAACGAGGGGCACATGCCTTCAAATTTTTCTACCATTCCCAAGCCCGACACCAAGAAATTCAACCATTCATTCATCAAATTAGGTTGGAATAGTTGTATTTCGGACACAAGATTATCGCTTCATTTCGCTGCTGTTCATATCGCCAGGGGATTTTGTGGAGTATCAGAAAATGGGCACTCGGAGAAATGTTTAAATGATAATGCAAGTGATGGTGATGTTGTTGCTAATAAAGTTCAAGGCGTCCATAGAAACTCTGTGGACTTCACCAAAATTCCTATTAATATGCTTCCAACTGTTGTGCTCGTTGGGCGCCCAAATGTTGGCAAGTCTGCATTATTTAACCGGTTTATTAGAAGGAGGGAGGCTCTAGTGTACAACACTCCAGATGATCACGTTACTCGGGATATTCGGGAAGAGCTGGTCTTCACCCCTTTGATTTGGAGGTTGGGAAGTGGTTTGCATGCAGAGACATGCACCGATATTAACATCGTGGTAGCAATGAATAAATCAATCGTTTTTGACAATGATGTGCACACTTTTTTGGTCTCTTTGCTCTCCGAAGCCCAAAGGTTAGGCTTTGGAGATCCAGTTCCTATATCAGCTGAAACTGGACTTGGCATGCAAGATCTTTATTGGGCCATCAAACCTGTGCTTGAGAAGTATATGTTGAAAGTTATAAATGATAATGGTGGTCTTGATCGCTTCCATCTAGTCACAAGCTCCAATGAGGAGGAGGACACCCAGGACAGTAAGGTGCCATTACAGTTAGCAATTGTTGGACGACCTAATGTTGGCAAGTCAACCTTGCTGAACACACTGTTGCAATCGGATCGTGTTTTGGTTGGTCCTGAAGCTGGTTTAACAAGGGATTCAGTTCGAGCTCAATTTGAGTTTGAGGGCAGAACTATATATTTGGTTGATACAGCTGGTTGGTTGCATAGGACTAAGGAAGAGAAAGGACCAGGATCCTTGAGTGTCATGCAATCGAGTAAGAATCTGATGAGAGCCCATGTGGTTGCTTTGGTTCTTGATGCAGAAGAGCGTGATTCCAATTTTCTTCTACGGAAAAGTAGAAGATTGTTGGTAGAAGTTGAAACGTTAACATGGCTTTTAAATCGGCATCCGTGCAACAGAATACGGTTTATTCATTTCAAAGACTTGAAGGAAGAGGCGGACTTTGCGATCATGCGGCGAGTTATTGAGACATGTGAAAAATGGTGTTCGAGGTTGTCCACAGCTCGTCTTAACCGGTGGTTGCGGAAGGTCAGCAGAAAATACCCTTTTGTTTTCTTAGCTCAGCATATGTATAGCACAATTTGTAAAAGCTCGGGCTTGCACAGAAAACAAATTGATGGGGTTGGCAAGAGCAGCAGACACGTTAACCGAACACTTGAAAGAATCCGATCGGATAAGAGACGACCCATCGTTGAAGAACAGACAGCTTGA

Coding sequence (CDS)

ATGTCTCGCTTATGGGTTCCATTGGTCTTCCAACCCCGTGGGTCATCGGCAACGACGTTTTCCATATCGGTAGAACAAGTAGAAAGAACAAGCTCAAATACTCTCTCTCTCAATTTTCTCAGTGGCTCGGTTTTATGCAACGAGGGGCACATGCCTTCAAATTTTTCTACCATTCCCAAGCCCGACACCAAGAAATTCAACCATTCATTCATCAAATTAGGTTGGAATAGTTGTATTTCGGACACAAGATTATCGCTTCATTTCGCTGCTGTTCATATCGCCAGGGGATTTTGTGGAGTATCAGAAAATGGGCACTCGGAGAAATGTTTAAATGATAATGCAAGTGATGGTGATGTTGTTGCTAATAAAGTTCAAGGCGTCCATAGAAACTCTGTGGACTTCACCAAAATTCCTATTAATATGCTTCCAACTGTTGTGCTCGTTGGGCGCCCAAATGTTGGCAAGTCTGCATTATTTAACCGGTTTATTAGAAGGAGGGAGGCTCTAGTGTACAACACTCCAGATGATCACGTTACTCGGGATATTCGGGAAGAGCTGGTCTTCACCCCTTTGATTTGGAGGTTGGGAAGTGGTTTGCATGCAGAGACATGCACCGATATTAACATCGTGGTAGCAATGAATAAATCAATCGTTTTTGACAATGATGTGCACACTTTTTTGGTCTCTTTGCTCTCCGAAGCCCAAAGGTTAGGCTTTGGAGATCCAGTTCCTATATCAGCTGAAACTGGACTTGGCATGCAAGATCTTTATTGGGCCATCAAACCTGTGCTTGAGAAGTATATGTTGAAAGTTATAAATGATAATGGTGGTCTTGATCGCTTCCATCTAGTCACAAGCTCCAATGAGGAGGAGGACACCCAGGACAGTAAGGTGCCATTACAGTTAGCAATTGTTGGACGACCTAATGTTGGCAAGTCAACCTTGCTGAACACACTGTTGCAATCGGATCGTGTTTTGGTTGGTCCTGAAGCTGGTTTAACAAGGGATTCAGTTCGAGCTCAATTTGAGTTTGAGGGCAGAACTATATATTTGGTTGATACAGCTGGTTGGTTGCATAGGACTAAGGAAGAGAAAGGACCAGGATCCTTGAGTGTCATGCAATCGAGTAAGAATCTGATGAGAGCCCATGTGGTTGCTTTGGTTCTTGATGCAGAAGAGCGTGATTCCAATTTTCTTCTACGGAAAAGTAGAAGATTGTTGGTAGAAGTTGAAACGTTAACATGGCTTTTAAATCGGCATCCGTGCAACAGAATACGGTTTATTCATTTCAAAGACTTGAAGGAAGAGGCGGACTTTGCGATCATGCGGCGAGTTATTGAGACATGTGAAAAATGGTGTTCGAGGTTGTCCACAGCTCGTCTTAACCGGTGGTTGCGGAAGGTCAGCAGAAAATACCCTTTTGTTTTCTTAGCTCAGCATATGTATAGCACAATTTGTAAAAGCTCGGGCTTGCACAGAAAACAAATTGATGGGGTTGGCAAGAGCAGCAGACACGTTAACCGAACACTTGAAAGAATCCGATCGGATAAGAGACGACCCATCGTTGAAGAACAGACAGCTTGA

Protein sequence

MSRLWVPLVFQPRGSSATTFSISVEQVERTSSNTLSLNFLSGSVLCNEGHMPSNFSTIPKPDTKKFNHSFIKLGWNSCISDTRLSLHFAAVHIARGFCGVSENGHSEKCLNDNASDGDVVANKVQGVHRNSVDFTKIPINMLPTVVLVGRPNVGKSALFNRFIRRREALVYNTPDDHVTRDIREELVFTPLIWRLGSGLHAETCTDINIVVAMNKSIVFDNDVHTFLVSLLSEAQRLGFGDPVPISAETGLGMQDLYWAIKPVLEKYMLKVINDNGGLDRFHLVTSSNEEEDTQDSKVPLQLAIVGRPNVGKSTLLNTLLQSDRVLVGPEAGLTRDSVRAQFEFEGRTIYLVDTAGWLHRTKEEKGPGSLSVMQSSKNLMRAHVVALVLDAEERDSNFLLRKSRRLLVEVETLTWLLNRHPCNRIRFIHFKDLKEEADFAIMRRVIETCEKWCSRLSTARLNRWLRKVSRKYPFVFLAQHMYSTICKSSGLHRKQIDGVGKSSRHVNRTLERIRSDKRRPIVEEQTA
Homology
BLAST of Tan0008543 vs. ExPASy Swiss-Prot
Match: Q1MDD6 (GTPase Der OS=Rhizobium leguminosarum bv. viciae (strain 3841) OX=216596 GN=der PE=3 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 1.4e-37
Identity = 131/404 (32.43%), Postives = 189/404 (46.78%), Query Frame = 0

Query: 144 TVVLVGRPNVGKSALFNRFIRRREALVYNTPDDHVTRDIR-------------------E 203
           TV +VGRPNVGKS LFNR + ++ ALV +TP   VTRD R                   E
Sbjct: 4   TVAIVGRPNVGKSTLFNRLVGKKLALVDDTPG--VTRDRRPGDARLMGLTFTIIDTAGLE 63

Query: 204 ELVFTPLIWRLGSGLHA---ETCTDINIVVAMNKSIVFDNDV---------HTFLVSLLS 263
           E     L  R+ +   A   E    + +V A +     D D+            LV+  S
Sbjct: 64  EADAESLQGRMRAQTEAAIDEADLSLFVVDAKSGLTPVDTDLAEMLRRRGKPVVLVANKS 123

Query: 264 EAQ----------RLGFGDPVPISAETGLGMQDLYWAIKPVLEKYMLKVINDNGGLDRFH 323
           EA+           LG G+P PISAE G GM DL  AI   + +       ++  +    
Sbjct: 124 EARGSDSGFYDAYTLGLGEPTPISAEHGQGMLDLRDAIVAAIGEDRAYPEKEDVAVTDVD 183

Query: 324 LVTSSNE----EEDTQDSKVPLQLAIVGRPNVGKSTLLNTLLQSDRVLVGPEAGLTRDSV 383
           +  SS+E    EE   D   PL++AIVGRPN GKSTL+N  L  DR+L GPEAG+TRDS+
Sbjct: 184 IPQSSDEGDEDEEPIYDDTKPLRVAIVGRPNAGKSTLINRFLGEDRLLTGPEAGITRDSI 243

Query: 384 RAQFEFEGRTIYLVDTAGWLHRTKEEKGPGSLSVMQSSKNLMRAHVVALVLDA----EER 443
             ++++ GRTI + DTAG   + +  +    LSV  + + +  A  V +V DA    E++
Sbjct: 244 SVEWDWRGRTIKMFDTAGMRRKARVIEKLEKLSVADALRAIRFAETVVIVFDATIPFEKQ 303

Query: 444 D---SNFLLRKSRRLLVEVETLTWLLNRHPCNRIRFIHFKDLKEEAD------------- 474
           D    + +LR+ R  ++       + +R            DL+E+ D             
Sbjct: 304 DLQIVDLVLREGRAAVLAFNKWDMIEDRQAV-------LADLREKTDRLLPQARGIRAVP 363

BLAST of Tan0008543 vs. ExPASy Swiss-Prot
Match: B0CK66 (GTPase Der OS=Brucella suis (strain ATCC 23445 / NCTC 10510) OX=470137 GN=der PE=3 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 1.8e-37
Identity = 129/404 (31.93%), Postives = 191/404 (47.28%), Query Frame = 0

Query: 144 TVVLVGRPNVGKSALFNRFIRRREALVYNTP--------DDHVTRDIREELVFTP-LIWR 203
           T+ +VGRPNVGKS LFNR + R+ ALV + P         D    D++ +L+ T  L   
Sbjct: 4   TLAIVGRPNVGKSTLFNRLVGRKLALVDDLPGVTRDRRIHDAKLYDLKFQLIDTAGLEEA 63

Query: 204 LGSGLHAETCTDINIVVAMNKSIVFDNDV--------HTF------------LVSLLSEA 263
               L A         ++   +++F  D          TF            LV+  +EA
Sbjct: 64  ANDSLEARMRAQTEAAISEADAVLFVIDAKAGITPADSTFAEAVRRSGKPVVLVANKAEA 123

Query: 264 Q----------RLGFGDPVPISAETGLGMQDLYWAIKPVL--EKYMLKVINDNGGLDRF- 323
           +          +LG G+P PISAE G GM DL  AI  +L  E+   +   +    + F 
Sbjct: 124 RGSEAGMYDAFQLGLGEPCPISAEHGQGMPDLRDAIVELLGEERVFAEERQEEAADEVFT 183

Query: 324 -----HLVTSSNEEEDTQ-----DSKVPLQLAIVGRPNVGKSTLLNTLLQSDRVLVGPEA 383
                 LV    E+ D +     D+  PL++AIVGRPN GKSTL+NT+L  DR+L GPEA
Sbjct: 184 PAAVGALVGDDIEDPDAEEIPAYDATKPLRIAIVGRPNAGKSTLINTMLGEDRLLTGPEA 243

Query: 384 GLTRDSVRAQFEFEGRTIYLVDTAGWLHRTKEEKGPGSLSVMQSSKNLMRAHVVALVLDA 443
           G+TRDS+ A +E+ GR I L DTAG   + + ++    LSV    + +  A VV +VLDA
Sbjct: 244 GITRDSISADWEWHGRKIKLFDTAGMRRKARVQEKLEKLSVADGLRAIRFAEVVIIVLDA 303

Query: 444 ----EERD---SNFLLRKSRRLLVEVETLTWLLNRH---------------PCNRIRFIH 474
               E++D   ++ ++R+ R  ++       + +R                    +R + 
Sbjct: 304 TIPFEKQDLQIADLIIREGRAPVIAFNKWDLIEDRQMVLADLYEKTARLLPQVRGLRAVP 363

BLAST of Tan0008543 vs. ExPASy Swiss-Prot
Match: Q2K5L2 (GTPase Der OS=Rhizobium etli (strain CFN 42 / ATCC 51251) OX=347834 GN=der PE=3 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 1.8e-37
Identity = 134/405 (33.09%), Postives = 185/405 (45.68%), Query Frame = 0

Query: 144 TVVLVGRPNVGKSALFNRFIRRREALVYNTPDDHVTRDIR-------------------E 203
           TV +VGRPNVGKS LFNR + ++ ALV +TP   VTRD R                   E
Sbjct: 4   TVAIVGRPNVGKSTLFNRLVGKKLALVDDTPG--VTRDRRPGDARLMGLTFTIIDTAGLE 63

Query: 204 ELVFTPLIWRLGSGLHA---ETCTDINIVVAMNKSIVFDNDV---------HTFLVSLLS 263
           E     L  R+ +   A   E    + +V A N     D  +            LV+  S
Sbjct: 64  EADEESLQGRMRAQTEAAIDEADLSLFVVDAKNGLTPVDTALAEMLRRRGKPVVLVANKS 123

Query: 264 EAQ----------RLGFGDPVPISAETGLGMQDLYWAIKPVLEKYMLKVINDNGGLDRFH 323
           EA+           LG G+P PISAE G GM DL  AI   + K       D    D   
Sbjct: 124 EARGSDSGFYDAYTLGLGEPTPISAEHGQGMLDLRDAIVEAIGKDRAYAKEDVAVTD-VD 183

Query: 324 LVTSSNE-----EEDTQDSKVPLQLAIVGRPNVGKSTLLNTLLQSDRVLVGPEAGLTRDS 383
           +  S NE     EE   D   PL++AIVGRPN GKSTL+N  L  DR+L GPEAG+TRDS
Sbjct: 184 IPPSENEADGEDEEPAYDETKPLRVAIVGRPNAGKSTLINRFLGEDRLLTGPEAGITRDS 243

Query: 384 VRAQFEFEGRTIYLVDTAGWLHRTKEEKGPGSLSVMQSSKNLMRAHVVALVLDA----EE 443
           +  ++++ GRTI + DTAG   + +  +    LSV  + + +  A  V +V DA    E+
Sbjct: 244 ISVEWDWRGRTIKMFDTAGMRRKARVTEKLEKLSVADALRAIRFAETVVIVFDATIPFEK 303

Query: 444 RD---SNFLLRKSRRLLVEVETLTWLLNRHPCNRIRFIHFKDLKEEAD------------ 474
           +D    + +LR+ R  ++       + +R            DL+E+ D            
Sbjct: 304 QDLQIVDLVLREGRAAVLAFNKWDMIEDRQAV-------LADLREKTDRLLPQARGIRAV 363

BLAST of Tan0008543 vs. ExPASy Swiss-Prot
Match: A6WW65 (GTPase Der OS=Ochrobactrum anthropi (strain ATCC 49188 / DSM 6882 / JCM 21032 / NBRC 15819 / NCTC 12168) OX=439375 GN=der PE=3 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 2.4e-37
Identity = 133/409 (32.52%), Postives = 190/409 (46.45%), Query Frame = 0

Query: 144 TVVLVGRPNVGKSALFNRFIRRREALVYNTPDDHVTRDIR-------------------E 203
           TV +VGRPNVGKS LFNR + R+ ALV + P   VTRD R                   E
Sbjct: 4   TVAIVGRPNVGKSTLFNRLVGRKLALVDDLPG--VTRDRRIHDAKLYDLKFQVIDTAGLE 63

Query: 204 ELVFTPLIWRLGSGLHA---ETCTDINIVVAMN---------KSIVFDNDVHTFLVSLLS 263
           E     L  R+ +   A   E    + +V A N           +V  +     LV+  +
Sbjct: 64  EAANDSLEARMRAQTEAAIQEADVILFVVDAKNGLTPTDSTFAEVVRRSGKPVVLVANKA 123

Query: 264 EAQ----------RLGFGDPVPISAETGLGMQDLYWAIKPVL----------------EK 323
           EA+          +LG G+P PISAE G GM DL  AI  +L                E 
Sbjct: 124 EARGAQSGMYDAFQLGLGEPCPISAEHGQGMPDLRDAIVELLGEERVFADEKEEETADEV 183

Query: 324 YMLKVINDNGGLDRFHLVTSSNEEEDTQDSKVPLQLAIVGRPNVGKSTLLNTLLQSDRVL 383
           +  K + +  G D   +     EE    D+  PL++AIVGRPN GKSTL+NT+L  DR+L
Sbjct: 184 FTPKAVGELIGDD---IEDPDEEEIPAYDATKPLRIAIVGRPNAGKSTLINTMLGEDRLL 243

Query: 384 VGPEAGLTRDSVRAQFEFEGRTIYLVDTAGWLHRTKEEKGPGSLSVMQSSKNLMRAHVVA 443
            GPEAG+TRDS+   +E+ GR I L DTAG   +++ ++    LSV    + +  A VV 
Sbjct: 244 TGPEAGITRDSISVDWEWRGRKIKLFDTAGLRRKSRVQEKLEKLSVADGLRAIRFAEVVI 303

Query: 444 LVLDA----EERD---SNFLLRKSRRLLVEVETLTWLLNRH---------------PCNR 474
           +VLDA    E++D   ++ ++R+ R  ++       + +R                    
Sbjct: 304 IVLDATIPFEKQDLQIADLIIREGRAPIIAFNKWDLIEDRQMVLADLYEKTARLLPQVRG 363

BLAST of Tan0008543 vs. ExPASy Swiss-Prot
Match: A9M8F4 (GTPase Der OS=Brucella canis (strain ATCC 23365 / NCTC 10854) OX=483179 GN=der PE=3 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 4.1e-37
Identity = 130/406 (32.02%), Postives = 190/406 (46.80%), Query Frame = 0

Query: 144 TVVLVGRPNVGKSALFNRFIRRREALVYNTPDDHVTRDIR-----------EELVFTPLI 203
           T+ +VGRPNVGKS LFNR + R+ ALV + P   VTRD R           + +    L 
Sbjct: 4   TLAIVGRPNVGKSTLFNRLVGRKLALVDDLPG--VTRDRRIHDAKLYDLKFQVIDTAGLE 63

Query: 204 WRLGSGLHAETCTDINIVVAMNKSIVFDNDV--------HTF------------LVSLLS 263
                 L A         ++   +++F  D          TF            LV+  +
Sbjct: 64  EAANDSLEARMRAQTEAAISEADAVLFVIDAKAGITPADSTFAEAVRRSGKPVVLVANKA 123

Query: 264 EAQ----------RLGFGDPVPISAETGLGMQDLYWAIKPVL--EKYMLKVINDNGGLDR 323
           EA+          +LG G+P PISAE G GM DL  AI  +L  E+   +   +    + 
Sbjct: 124 EARGSEAGMYDAFQLGLGEPCPISAEHGQGMPDLRDAIVELLGEERVFAEERQEEAADEV 183

Query: 324 F------HLVTSSNEEEDTQ-----DSKVPLQLAIVGRPNVGKSTLLNTLLQSDRVLVGP 383
           F       LV    E+ D +     D+  PL++AIVGRPN GKSTL+NT+L  DR+L GP
Sbjct: 184 FTPAAVGALVGDDIEDPDAEEIPAYDATKPLRIAIVGRPNAGKSTLINTMLGEDRLLTGP 243

Query: 384 EAGLTRDSVRAQFEFEGRTIYLVDTAGWLHRTKEEKGPGSLSVMQSSKNLMRAHVVALVL 443
           EAG+TRDS+ A +E+ GR I L DTAG   + + ++    LSV    + +  A VV +VL
Sbjct: 244 EAGITRDSISADWEWHGRKIKLFDTAGMRRKARVQEKLEKLSVADGLRAIRFAEVVIIVL 303

Query: 444 DA----EERD---SNFLLRKSRRLLVEVETLTWLLNRH---------------PCNRIRF 474
           DA    E++D   ++ ++R+ R  ++       + +R                    +R 
Sbjct: 304 DATIPFEKQDLQIADLIIREGRAPVIAFNKWDLIEDRQMVLADLYEKTARLLPQVRGLRA 363

BLAST of Tan0008543 vs. NCBI nr
Match: XP_022984952.1 (uncharacterized protein LOC111483067 [Cucurbita maxima])

HSP 1 Score: 617.8 bits (1592), Expect = 8.4e-173
Identity = 373/656 (56.86%), Postives = 413/656 (62.96%), Query Frame = 0

Query: 1   MSRLWVPLVFQPRGSSATTFSISVEQVERTSSNTLSLNFLSGSVLCNEGHMPSNFSTIPK 60
           MSRLW PL+ Q R       S  V +   TS ++L  NFLSGSVLCN GH PSNFST+PK
Sbjct: 1   MSRLWFPLLSQRRRVIGNGVSDIVGRSRTTSFSSLLPNFLSGSVLCNGGHTPSNFSTLPK 60

Query: 61  PDTKKFNHSFIKLGWNSCISDTRLSLHFAAVHIARGFCGVSENGHSEKCLNDNASDGDVV 120
           P ++K NHSF+K GWNS IS TRL L  AAVHIARGFCGVSENGHSEK LND ASDGDVV
Sbjct: 61  PLSEKCNHSFVKSGWNSGISSTRLLLPLAAVHIARGFCGVSENGHSEKFLNDKASDGDVV 120

Query: 121 ANKVQGVHRNSVDFTKIPINMLPTVVLVGRPNVGKSALFNRFIRRREALVYNTPDDHVTR 180
           AN VQ VH+NSVDFTKIPINMLPTVVLVGRPNVGKSALFNRFIRRREALVYNTPDDHVTR
Sbjct: 121 ANIVQAVHKNSVDFTKIPINMLPTVVLVGRPNVGKSALFNRFIRRREALVYNTPDDHVTR 180

Query: 181 DIRE------ELVFTPL----------------------------------IWRLGSGLH 240
           DIRE      +L F  L                                  +    +GLH
Sbjct: 181 DIREGVAKLGDLRFVVLDSSGLETAVSSGSILERTARMTEKVLLKCQLAIFLIDARAGLH 240

Query: 241 ----------AETCTDINIVVAMNKSIVFDNDVHTFLVSLLSEAQRLGFGDPVPISAETG 300
                          DINI+VAMNKS    +   T L +   EA+RLGFGDPVPISAETG
Sbjct: 241 PFDLEVGKWLRRNVPDINILVAMNKSESLIDSSGTLLAAGF-EAERLGFGDPVPISAETG 300

Query: 301 LGMQDLYWAIKPVLEKYMLKVINDNGGLDRFHLVTSSNEEEDTQDSKVPLQLAIVGRPNV 360
           LGM DLY AIKPVLEKYMLKVINDNGGLDRFH VT SNE+EDTQDSKV LQLAIVGRPNV
Sbjct: 301 LGMHDLYLAIKPVLEKYMLKVINDNGGLDRFHQVTGSNEDEDTQDSKVQLQLAIVGRPNV 360

Query: 361 GKSTLLNTLLQSDRVLVGPEAGLTRDSVRAQFEFEGRTIYLVDTAGWLHRTKEEKGPGSL 420
           GKSTLLNTLLQSDRVLVGPEAGLTRDSVRAQFEFEGRTIYLVDTAGWLHRTKEEKGP SL
Sbjct: 361 GKSTLLNTLLQSDRVLVGPEAGLTRDSVRAQFEFEGRTIYLVDTAGWLHRTKEEKGPSSL 420

Query: 421 SVMQSSKNLMRAHVVALVLDAEE--RDSNFLLRK----SRRLLVEVETLTWLLNRHPCNR 480
           SVMQS+KNLMRAHVVALVLDAEE  R+   +       +RR + E   L  ++N+    R
Sbjct: 421 SVMQSTKNLMRAHVVALVLDAEEIARERRSMKHAEVVIARRAVEEGRGLVIIVNKMDLMR 480

Query: 481 ----------------------------IRFIHFKDLKEEADFAIMRRVIETCEKWCSRL 528
                                       I  I    L+     A+MR+V+ET EKWC RL
Sbjct: 481 GRKNSASYEKILEAVPEEIQTVIPQVTGIPVIFISALEGRGRLAVMRQVVETYEKWCLRL 540

BLAST of Tan0008543 vs. NCBI nr
Match: XP_023552191.1 (uncharacterized protein LOC111809943 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 614.8 bits (1584), Expect = 7.1e-172
Identity = 372/656 (56.71%), Postives = 412/656 (62.80%), Query Frame = 0

Query: 1   MSRLWVPLVFQPRGSSATTFSISVEQVERTSSNTLSLNFLSGSVLCNEGHMPSNFSTIPK 60
           MSRLW PL+ Q R       S  V +   TS + L  NFLSGSVLCN GH PSNFST+PK
Sbjct: 1   MSRLWFPLLSQRRRVIGNGVSGIVGRSRTTSFSCLLPNFLSGSVLCNGGHTPSNFSTLPK 60

Query: 61  PDTKKFNHSFIKLGWNSCISDTRLSLHFAAVHIARGFCGVSENGHSEKCLNDNASDGDVV 120
           P ++K NHSF+K GWNS IS TR  L  AAVHIARGFCGVSENGHSEKCLND ASDGDVV
Sbjct: 61  PLSEKCNHSFVKSGWNSGISSTRSLLPLAAVHIARGFCGVSENGHSEKCLNDKASDGDVV 120

Query: 121 ANKVQGVHRNSVDFTKIPINMLPTVVLVGRPNVGKSALFNRFIRRREALVYNTPDDHVTR 180
           AN VQ V++NSVDFTKIPIN LPTVVLVGRPNVGKSALFNRFIRRREALVYNTPDDHVTR
Sbjct: 121 ANIVQAVYKNSVDFTKIPINRLPTVVLVGRPNVGKSALFNRFIRRREALVYNTPDDHVTR 180

Query: 181 DIRE------ELVFTPL----------------------------------IWRLGSGLH 240
           DIRE      +L F  L                                  +    +GLH
Sbjct: 181 DIREGVAKLGDLRFVVLDSSGLETAVSSGSILERTAKMTEKVLLKCQLAIFLIDARAGLH 240

Query: 241 ----------AETCTDINIVVAMNKSIVFDNDVHTFLVSLLSEAQRLGFGDPVPISAETG 300
                          DINI+VAMNKS    +   T L +   EA+RLGFGDPVPISAETG
Sbjct: 241 PFDLEVGKWLRRHVPDINILVAMNKSESLIDCSGTLLAAGF-EAERLGFGDPVPISAETG 300

Query: 301 LGMQDLYWAIKPVLEKYMLKVINDNGGLDRFHLVTSSNEEEDTQDSKVPLQLAIVGRPNV 360
           LGM DLY AIKPVLEKYMLKVINDNGGLDRFH VT SNE+EDTQDSKV LQLAIVGRPNV
Sbjct: 301 LGMHDLYLAIKPVLEKYMLKVINDNGGLDRFHQVTGSNEDEDTQDSKVQLQLAIVGRPNV 360

Query: 361 GKSTLLNTLLQSDRVLVGPEAGLTRDSVRAQFEFEGRTIYLVDTAGWLHRTKEEKGPGSL 420
           GKSTLLNTLLQSDRVLVGPEAGLTRDSVRAQFEFEGRTIYLVDTAGWLHRTKEEKGP SL
Sbjct: 361 GKSTLLNTLLQSDRVLVGPEAGLTRDSVRAQFEFEGRTIYLVDTAGWLHRTKEEKGPSSL 420

Query: 421 SVMQSSKNLMRAHVVALVLDAEE--RDSNFLLRK----SRRLLVEVETLTWLLNRHPCNR 480
           SVMQS+KNLMRAHVVALVLDAEE  R+   +       +RR + E   L  ++N+    R
Sbjct: 421 SVMQSTKNLMRAHVVALVLDAEEIARERRSMKHAEVVIARRAVEEGRGLVIIVNKMDLMR 480

Query: 481 ----------------------------IRFIHFKDLKEEADFAIMRRVIETCEKWCSRL 528
                                       I  I    L+     A+MR+V+ET EKWC RL
Sbjct: 481 GRKNSASYEKILEAVPEEIQTVIPQVTGIPVIFISALEGRGRLAVMRQVVETYEKWCLRL 540

BLAST of Tan0008543 vs. NCBI nr
Match: KAG6577347.1 (hypothetical protein SDJN03_24921, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 612.1 bits (1577), Expect = 4.6e-171
Identity = 370/656 (56.40%), Postives = 411/656 (62.65%), Query Frame = 0

Query: 1   MSRLWVPLVFQPRGSSATTFSISVEQVERTSSNTLSLNFLSGSVLCNEGHMPSNFSTIPK 60
           MSRLW PL+ Q R       S  V +   TS + L  NFLSGSVLCN GH PSNFST+P+
Sbjct: 1   MSRLWFPLLSQRRRVIGNGVSGIVGRSRTTSFSCLLPNFLSGSVLCNGGHTPSNFSTLPE 60

Query: 61  PDTKKFNHSFIKLGWNSCISDTRLSLHFAAVHIARGFCGVSENGHSEKCLNDNASDGDVV 120
           P ++K NHSF+K GWNS  S TRL L  AAVHIARGFCGVSENGHSEK LND A DGDVV
Sbjct: 61  PLSEKCNHSFVKSGWNSGFSSTRLLLPLAAVHIARGFCGVSENGHSEKFLNDKARDGDVV 120

Query: 121 ANKVQGVHRNSVDFTKIPINMLPTVVLVGRPNVGKSALFNRFIRRREALVYNTPDDHVTR 180
           AN VQ VH+NSVDFTKIPINMLPTVVLVGRPNVGKSALFNRFIRRREALVYNTPDDHVTR
Sbjct: 121 ANIVQAVHKNSVDFTKIPINMLPTVVLVGRPNVGKSALFNRFIRRREALVYNTPDDHVTR 180

Query: 181 DIRE------ELVFTPL----------------------------------IWRLGSGLH 240
           DIRE      +L F  L                                  +    +GLH
Sbjct: 181 DIREGVAKLGDLRFVVLDSSGLETAVSSGSILERTAKMTEKVLLKCQLAIFLIDARAGLH 240

Query: 241 ----------AETCTDINIVVAMNKSIVFDNDVHTFLVSLLSEAQRLGFGDPVPISAETG 300
                          DINI+VAMNKS    +   T L +   EA+RLGFGDPVPISAETG
Sbjct: 241 PFDLEVGKWLRRHVPDINILVAMNKSESLIDCSGTLLAAGF-EAERLGFGDPVPISAETG 300

Query: 301 LGMQDLYWAIKPVLEKYMLKVINDNGGLDRFHLVTSSNEEEDTQDSKVPLQLAIVGRPNV 360
           LGM DLY AIKPVLEKYMLKVINDNGGLDRFH VT SNE+EDTQDSKV LQLAIVGRPNV
Sbjct: 301 LGMHDLYLAIKPVLEKYMLKVINDNGGLDRFHQVTGSNEDEDTQDSKVQLQLAIVGRPNV 360

Query: 361 GKSTLLNTLLQSDRVLVGPEAGLTRDSVRAQFEFEGRTIYLVDTAGWLHRTKEEKGPGSL 420
           GKSTLLNTLLQSDRVLVGPEAGLTRDSVRAQFEFEGRTIYLVDTAGWLHRTKEEKGP SL
Sbjct: 361 GKSTLLNTLLQSDRVLVGPEAGLTRDSVRAQFEFEGRTIYLVDTAGWLHRTKEEKGPSSL 420

Query: 421 SVMQSSKNLMRAHVVALVLDAEE--RDSNFLLRK----SRRLLVEVETLTWLLNRHPCNR 480
           SVMQS+KNLMRAHVVALVLDAEE  R+   +       +RR + E   L  ++N+    R
Sbjct: 421 SVMQSTKNLMRAHVVALVLDAEEIARERRSMKHAEVVIARRAVEEGRGLVIIVNKMDLMR 480

Query: 481 ----------------------------IRFIHFKDLKEEADFAIMRRVIETCEKWCSRL 528
                                       I  I    L+     A+MR+V+ET EKWC RL
Sbjct: 481 GRKNSASYEKILEAVPEEVQTVIPQVTGIPVIFISALEGRGRLAVMRQVVETYEKWCLRL 540

BLAST of Tan0008543 vs. NCBI nr
Match: XP_022929494.1 (uncharacterized protein LOC111436046 [Cucurbita moschata])

HSP 1 Score: 610.9 bits (1574), Expect = 1.0e-170
Identity = 369/656 (56.25%), Postives = 411/656 (62.65%), Query Frame = 0

Query: 1   MSRLWVPLVFQPRGSSATTFSISVEQVERTSSNTLSLNFLSGSVLCNEGHMPSNFSTIPK 60
           MSRLW PL+ Q R       S  V +   TS + L  NFLSGSVLCN GH PSNFST+PK
Sbjct: 1   MSRLWFPLLSQRRRVIGNGVSGIVGRSRTTSFSCLLPNFLSGSVLCNGGHTPSNFSTLPK 60

Query: 61  PDTKKFNHSFIKLGWNSCISDTRLSLHFAAVHIARGFCGVSENGHSEKCLNDNASDGDVV 120
           P ++K NHSF+K GWNS  S TRL L  AAVHIARGFCGVSENGHSEK LND A DGDVV
Sbjct: 61  PLSEKCNHSFVKSGWNSGFSSTRLLLPLAAVHIARGFCGVSENGHSEKFLNDKARDGDVV 120

Query: 121 ANKVQGVHRNSVDFTKIPINMLPTVVLVGRPNVGKSALFNRFIRRREALVYNTPDDHVTR 180
           AN VQ VH+NS+DFTKIPINMLPTVVLVGRPNVGKSALFNRFIRRREALVYNTPDDHVTR
Sbjct: 121 ANIVQAVHKNSLDFTKIPINMLPTVVLVGRPNVGKSALFNRFIRRREALVYNTPDDHVTR 180

Query: 181 DIRE------ELVFTPL----------------------------------IWRLGSGLH 240
           DIRE      +L F  L                                  +    +GLH
Sbjct: 181 DIREGVAKLGDLRFVVLDSSGLETAVSSGSILERTAKMTEKVLLKCQLAIFLIDARAGLH 240

Query: 241 ----------AETCTDINIVVAMNKSIVFDNDVHTFLVSLLSEAQRLGFGDPVPISAETG 300
                          DINI+VAMNKS    +   T L +   EA+RLGFGDPVPISAETG
Sbjct: 241 PFDLEVGKWLRRHVPDINILVAMNKSESLIDCSGTLLAAGF-EAERLGFGDPVPISAETG 300

Query: 301 LGMQDLYWAIKPVLEKYMLKVINDNGGLDRFHLVTSSNEEEDTQDSKVPLQLAIVGRPNV 360
           LGM DLY AIKPVLEKYML+VINDNGGLDRFH VT SNE+EDTQDSKV LQLAIVGRPNV
Sbjct: 301 LGMHDLYLAIKPVLEKYMLEVINDNGGLDRFHQVTGSNEDEDTQDSKVQLQLAIVGRPNV 360

Query: 361 GKSTLLNTLLQSDRVLVGPEAGLTRDSVRAQFEFEGRTIYLVDTAGWLHRTKEEKGPGSL 420
           GKSTLLNTLLQSDRVLVGPEAGLTRDSVRAQFEFEGRTIYLVDTAGWLHRTKEEKGP SL
Sbjct: 361 GKSTLLNTLLQSDRVLVGPEAGLTRDSVRAQFEFEGRTIYLVDTAGWLHRTKEEKGPSSL 420

Query: 421 SVMQSSKNLMRAHVVALVLDAEE--RDSNFLLRK----SRRLLVEVETLTWLLNRHPCNR 480
           SVMQS+KNLMRAHVVALVLDAEE  R+   +       +RR + E   L  ++N+    R
Sbjct: 421 SVMQSTKNLMRAHVVALVLDAEEIARERRSMKHAEVVIARRAVEEGRGLVIIVNKMDLMR 480

Query: 481 ----------------------------IRFIHFKDLKEEADFAIMRRVIETCEKWCSRL 528
                                       I  I    L+     A+MR+V+ET EKWC RL
Sbjct: 481 GRKNSASYEKILEAVPEEVQTVIPQVTGIPVIFISALEGRGRLAVMRQVVETYEKWCLRL 540

BLAST of Tan0008543 vs. NCBI nr
Match: XP_022136758.1 (uncharacterized protein LOC111008384 [Momordica charantia])

HSP 1 Score: 596.3 bits (1536), Expect = 2.6e-166
Identity = 360/657 (54.79%), Postives = 406/657 (61.80%), Query Frame = 0

Query: 1   MSRLWVPLVFQPRGSSATTFSISVEQVERTSSNTLSLNFLSGSVLCNEGHMPSNFSTIPK 60
           MSRLWVPL+   R            +  RTSS+T++ NF SGSV C+EGH  S FS++P+
Sbjct: 1   MSRLWVPLLSHRRRVIGYGVFRIGGRSRRTSSSTVTPNFFSGSVFCSEGHTSSKFSSLPE 60

Query: 61  PDTKKFNHSFIKLGWNSCISDTRLSLHFAAVHIARGFCGVSENGHSEKCLNDNASDGDVV 120
           P ++K NHSF+  GWNSCIS+ RL +  AA  IARGFCG+SENG+SEK  NDNA D DV 
Sbjct: 61  PWSEKCNHSFVTSGWNSCISNPRLLVPLAAARIARGFCGLSENGNSEKRSNDNAMDSDVS 120

Query: 121 ANKVQGVHRNSVDFTKIPINMLPTVVLVGRPNVGKSALFNRFIRRREALVYNTPDDHVTR 180
            NKVQGVHRNSVDFTKI I+MLPTVVLVGRPNVGKSALFNRFI+RREALVYNTPDDHVTR
Sbjct: 121 GNKVQGVHRNSVDFTKISISMLPTVVLVGRPNVGKSALFNRFIKRREALVYNTPDDHVTR 180

Query: 181 DIRE------ELVFT--------------------------------------------- 240
           DIRE      +L F                                              
Sbjct: 181 DIREGVAKLGDLRFVVLDSSGLETTASSGSILERTARMTGNVLLKSQLAIFLIDARAGLH 240

Query: 241 PLIWRLGSGLHAETCTDINIVVAMNKSIVFDNDVHTFLVSLLSEAQRLGFGDPVPISAET 300
           P    +G  L   T  DI I+VAMNKS    +   T L + L EAQRLGFGDPVPISAET
Sbjct: 241 PFDLEVGKWLRRHT-PDIKIIVAMNKSESLFDSSGTLLAAAL-EAQRLGFGDPVPISAET 300

Query: 301 GLGMQDLYWAIKPVLEKYMLKVINDNGGLDRFHLVTSSNEEEDTQDSKVPLQLAIVGRPN 360
           GLGMQ+LY AIKPVLE YMLKVI+DNGGLDRFH VTSSNE+EDTQDSK+PLQLAIVGRPN
Sbjct: 301 GLGMQELYCAIKPVLENYMLKVIDDNGGLDRFHQVTSSNEDEDTQDSKLPLQLAIVGRPN 360

Query: 361 VGKSTLLNTLLQSDRVLVGPEAGLTRDSVRAQFEFEGRTIYLVDTAGWLHRTKEEKGPGS 420
           VGKSTLLNTLLQSDRVLVGPEAGLTRDSVRAQF+FE RTIYLVDTAGWLHRTKEEKGP S
Sbjct: 361 VGKSTLLNTLLQSDRVLVGPEAGLTRDSVRAQFQFEDRTIYLVDTAGWLHRTKEEKGPAS 420

Query: 421 LSVMQSSKNLMRAHVVALVLDAEE--RDSNFLLRK----SRRLLVEVETLTWLLNRHPCN 480
           LSVMQSSKNLMRAHVVALVLDAEE  R+   +       +RR + E   L  ++N+    
Sbjct: 421 LSVMQSSKNLMRAHVVALVLDAEEIARERRSMKHAEVVIARRAVEEGRGLVVIVNKMDLM 480

Query: 481 R----------------------------IRFIHFKDLKEEADFAIMRRVIETCEKWCSR 528
           R                            I  I    L+     A+MR+VIET EKWCSR
Sbjct: 481 RGKENFASYEKILEAVPEEIQTVIPQVTGIPVIFISALEGRGRLAVMRQVIETYEKWCSR 540

BLAST of Tan0008543 vs. ExPASy TrEMBL
Match: A0A6J1J3J0 (GTP-binding protein EngA OS=Cucurbita maxima OX=3661 GN=LOC111483067 PE=3 SV=1)

HSP 1 Score: 617.8 bits (1592), Expect = 4.1e-173
Identity = 373/656 (56.86%), Postives = 413/656 (62.96%), Query Frame = 0

Query: 1   MSRLWVPLVFQPRGSSATTFSISVEQVERTSSNTLSLNFLSGSVLCNEGHMPSNFSTIPK 60
           MSRLW PL+ Q R       S  V +   TS ++L  NFLSGSVLCN GH PSNFST+PK
Sbjct: 1   MSRLWFPLLSQRRRVIGNGVSDIVGRSRTTSFSSLLPNFLSGSVLCNGGHTPSNFSTLPK 60

Query: 61  PDTKKFNHSFIKLGWNSCISDTRLSLHFAAVHIARGFCGVSENGHSEKCLNDNASDGDVV 120
           P ++K NHSF+K GWNS IS TRL L  AAVHIARGFCGVSENGHSEK LND ASDGDVV
Sbjct: 61  PLSEKCNHSFVKSGWNSGISSTRLLLPLAAVHIARGFCGVSENGHSEKFLNDKASDGDVV 120

Query: 121 ANKVQGVHRNSVDFTKIPINMLPTVVLVGRPNVGKSALFNRFIRRREALVYNTPDDHVTR 180
           AN VQ VH+NSVDFTKIPINMLPTVVLVGRPNVGKSALFNRFIRRREALVYNTPDDHVTR
Sbjct: 121 ANIVQAVHKNSVDFTKIPINMLPTVVLVGRPNVGKSALFNRFIRRREALVYNTPDDHVTR 180

Query: 181 DIRE------ELVFTPL----------------------------------IWRLGSGLH 240
           DIRE      +L F  L                                  +    +GLH
Sbjct: 181 DIREGVAKLGDLRFVVLDSSGLETAVSSGSILERTARMTEKVLLKCQLAIFLIDARAGLH 240

Query: 241 ----------AETCTDINIVVAMNKSIVFDNDVHTFLVSLLSEAQRLGFGDPVPISAETG 300
                          DINI+VAMNKS    +   T L +   EA+RLGFGDPVPISAETG
Sbjct: 241 PFDLEVGKWLRRNVPDINILVAMNKSESLIDSSGTLLAAGF-EAERLGFGDPVPISAETG 300

Query: 301 LGMQDLYWAIKPVLEKYMLKVINDNGGLDRFHLVTSSNEEEDTQDSKVPLQLAIVGRPNV 360
           LGM DLY AIKPVLEKYMLKVINDNGGLDRFH VT SNE+EDTQDSKV LQLAIVGRPNV
Sbjct: 301 LGMHDLYLAIKPVLEKYMLKVINDNGGLDRFHQVTGSNEDEDTQDSKVQLQLAIVGRPNV 360

Query: 361 GKSTLLNTLLQSDRVLVGPEAGLTRDSVRAQFEFEGRTIYLVDTAGWLHRTKEEKGPGSL 420
           GKSTLLNTLLQSDRVLVGPEAGLTRDSVRAQFEFEGRTIYLVDTAGWLHRTKEEKGP SL
Sbjct: 361 GKSTLLNTLLQSDRVLVGPEAGLTRDSVRAQFEFEGRTIYLVDTAGWLHRTKEEKGPSSL 420

Query: 421 SVMQSSKNLMRAHVVALVLDAEE--RDSNFLLRK----SRRLLVEVETLTWLLNRHPCNR 480
           SVMQS+KNLMRAHVVALVLDAEE  R+   +       +RR + E   L  ++N+    R
Sbjct: 421 SVMQSTKNLMRAHVVALVLDAEEIARERRSMKHAEVVIARRAVEEGRGLVIIVNKMDLMR 480

Query: 481 ----------------------------IRFIHFKDLKEEADFAIMRRVIETCEKWCSRL 528
                                       I  I    L+     A+MR+V+ET EKWC RL
Sbjct: 481 GRKNSASYEKILEAVPEEIQTVIPQVTGIPVIFISALEGRGRLAVMRQVVETYEKWCLRL 540

BLAST of Tan0008543 vs. ExPASy TrEMBL
Match: A0A6J1ENX0 (GTP-binding protein EngA OS=Cucurbita moschata OX=3662 GN=LOC111436046 PE=3 SV=1)

HSP 1 Score: 610.9 bits (1574), Expect = 5.0e-171
Identity = 369/656 (56.25%), Postives = 411/656 (62.65%), Query Frame = 0

Query: 1   MSRLWVPLVFQPRGSSATTFSISVEQVERTSSNTLSLNFLSGSVLCNEGHMPSNFSTIPK 60
           MSRLW PL+ Q R       S  V +   TS + L  NFLSGSVLCN GH PSNFST+PK
Sbjct: 1   MSRLWFPLLSQRRRVIGNGVSGIVGRSRTTSFSCLLPNFLSGSVLCNGGHTPSNFSTLPK 60

Query: 61  PDTKKFNHSFIKLGWNSCISDTRLSLHFAAVHIARGFCGVSENGHSEKCLNDNASDGDVV 120
           P ++K NHSF+K GWNS  S TRL L  AAVHIARGFCGVSENGHSEK LND A DGDVV
Sbjct: 61  PLSEKCNHSFVKSGWNSGFSSTRLLLPLAAVHIARGFCGVSENGHSEKFLNDKARDGDVV 120

Query: 121 ANKVQGVHRNSVDFTKIPINMLPTVVLVGRPNVGKSALFNRFIRRREALVYNTPDDHVTR 180
           AN VQ VH+NS+DFTKIPINMLPTVVLVGRPNVGKSALFNRFIRRREALVYNTPDDHVTR
Sbjct: 121 ANIVQAVHKNSLDFTKIPINMLPTVVLVGRPNVGKSALFNRFIRRREALVYNTPDDHVTR 180

Query: 181 DIRE------ELVFTPL----------------------------------IWRLGSGLH 240
           DIRE      +L F  L                                  +    +GLH
Sbjct: 181 DIREGVAKLGDLRFVVLDSSGLETAVSSGSILERTAKMTEKVLLKCQLAIFLIDARAGLH 240

Query: 241 ----------AETCTDINIVVAMNKSIVFDNDVHTFLVSLLSEAQRLGFGDPVPISAETG 300
                          DINI+VAMNKS    +   T L +   EA+RLGFGDPVPISAETG
Sbjct: 241 PFDLEVGKWLRRHVPDINILVAMNKSESLIDCSGTLLAAGF-EAERLGFGDPVPISAETG 300

Query: 301 LGMQDLYWAIKPVLEKYMLKVINDNGGLDRFHLVTSSNEEEDTQDSKVPLQLAIVGRPNV 360
           LGM DLY AIKPVLEKYML+VINDNGGLDRFH VT SNE+EDTQDSKV LQLAIVGRPNV
Sbjct: 301 LGMHDLYLAIKPVLEKYMLEVINDNGGLDRFHQVTGSNEDEDTQDSKVQLQLAIVGRPNV 360

Query: 361 GKSTLLNTLLQSDRVLVGPEAGLTRDSVRAQFEFEGRTIYLVDTAGWLHRTKEEKGPGSL 420
           GKSTLLNTLLQSDRVLVGPEAGLTRDSVRAQFEFEGRTIYLVDTAGWLHRTKEEKGP SL
Sbjct: 361 GKSTLLNTLLQSDRVLVGPEAGLTRDSVRAQFEFEGRTIYLVDTAGWLHRTKEEKGPSSL 420

Query: 421 SVMQSSKNLMRAHVVALVLDAEE--RDSNFLLRK----SRRLLVEVETLTWLLNRHPCNR 480
           SVMQS+KNLMRAHVVALVLDAEE  R+   +       +RR + E   L  ++N+    R
Sbjct: 421 SVMQSTKNLMRAHVVALVLDAEEIARERRSMKHAEVVIARRAVEEGRGLVIIVNKMDLMR 480

Query: 481 ----------------------------IRFIHFKDLKEEADFAIMRRVIETCEKWCSRL 528
                                       I  I    L+     A+MR+V+ET EKWC RL
Sbjct: 481 GRKNSASYEKILEAVPEEVQTVIPQVTGIPVIFISALEGRGRLAVMRQVVETYEKWCLRL 540

BLAST of Tan0008543 vs. ExPASy TrEMBL
Match: A0A6J1C8F6 (GTP-binding protein EngA OS=Momordica charantia OX=3673 GN=LOC111008384 PE=3 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 1.3e-166
Identity = 360/657 (54.79%), Postives = 406/657 (61.80%), Query Frame = 0

Query: 1   MSRLWVPLVFQPRGSSATTFSISVEQVERTSSNTLSLNFLSGSVLCNEGHMPSNFSTIPK 60
           MSRLWVPL+   R            +  RTSS+T++ NF SGSV C+EGH  S FS++P+
Sbjct: 1   MSRLWVPLLSHRRRVIGYGVFRIGGRSRRTSSSTVTPNFFSGSVFCSEGHTSSKFSSLPE 60

Query: 61  PDTKKFNHSFIKLGWNSCISDTRLSLHFAAVHIARGFCGVSENGHSEKCLNDNASDGDVV 120
           P ++K NHSF+  GWNSCIS+ RL +  AA  IARGFCG+SENG+SEK  NDNA D DV 
Sbjct: 61  PWSEKCNHSFVTSGWNSCISNPRLLVPLAAARIARGFCGLSENGNSEKRSNDNAMDSDVS 120

Query: 121 ANKVQGVHRNSVDFTKIPINMLPTVVLVGRPNVGKSALFNRFIRRREALVYNTPDDHVTR 180
            NKVQGVHRNSVDFTKI I+MLPTVVLVGRPNVGKSALFNRFI+RREALVYNTPDDHVTR
Sbjct: 121 GNKVQGVHRNSVDFTKISISMLPTVVLVGRPNVGKSALFNRFIKRREALVYNTPDDHVTR 180

Query: 181 DIRE------ELVFT--------------------------------------------- 240
           DIRE      +L F                                              
Sbjct: 181 DIREGVAKLGDLRFVVLDSSGLETTASSGSILERTARMTGNVLLKSQLAIFLIDARAGLH 240

Query: 241 PLIWRLGSGLHAETCTDINIVVAMNKSIVFDNDVHTFLVSLLSEAQRLGFGDPVPISAET 300
           P    +G  L   T  DI I+VAMNKS    +   T L + L EAQRLGFGDPVPISAET
Sbjct: 241 PFDLEVGKWLRRHT-PDIKIIVAMNKSESLFDSSGTLLAAAL-EAQRLGFGDPVPISAET 300

Query: 301 GLGMQDLYWAIKPVLEKYMLKVINDNGGLDRFHLVTSSNEEEDTQDSKVPLQLAIVGRPN 360
           GLGMQ+LY AIKPVLE YMLKVI+DNGGLDRFH VTSSNE+EDTQDSK+PLQLAIVGRPN
Sbjct: 301 GLGMQELYCAIKPVLENYMLKVIDDNGGLDRFHQVTSSNEDEDTQDSKLPLQLAIVGRPN 360

Query: 361 VGKSTLLNTLLQSDRVLVGPEAGLTRDSVRAQFEFEGRTIYLVDTAGWLHRTKEEKGPGS 420
           VGKSTLLNTLLQSDRVLVGPEAGLTRDSVRAQF+FE RTIYLVDTAGWLHRTKEEKGP S
Sbjct: 361 VGKSTLLNTLLQSDRVLVGPEAGLTRDSVRAQFQFEDRTIYLVDTAGWLHRTKEEKGPAS 420

Query: 421 LSVMQSSKNLMRAHVVALVLDAEE--RDSNFLLRK----SRRLLVEVETLTWLLNRHPCN 480
           LSVMQSSKNLMRAHVVALVLDAEE  R+   +       +RR + E   L  ++N+    
Sbjct: 421 LSVMQSSKNLMRAHVVALVLDAEEIARERRSMKHAEVVIARRAVEEGRGLVVIVNKMDLM 480

Query: 481 R----------------------------IRFIHFKDLKEEADFAIMRRVIETCEKWCSR 528
           R                            I  I    L+     A+MR+VIET EKWCSR
Sbjct: 481 RGKENFASYEKILEAVPEEIQTVIPQVTGIPVIFISALEGRGRLAVMRQVIETYEKWCSR 540

BLAST of Tan0008543 vs. ExPASy TrEMBL
Match: A0A1S3BT83 (GTP-binding protein EngA OS=Cucumis melo OX=3656 GN=LOC103493287 PE=3 SV=1)

HSP 1 Score: 547.4 bits (1409), Expect = 6.7e-152
Identity = 344/654 (52.60%), Postives = 390/654 (59.63%), Query Frame = 0

Query: 1   MSRLWVPLVFQPRGSSATTFSISVEQVERTSSNTLSLNFLSGSVLCNEGHMPSNFSTIPK 60
           MSRLW PL+ Q R          VE   R  S+T S N LS SV                
Sbjct: 1   MSRLWAPLLSQRRRFIGNGVFHFVETSRRICSSTPSPNLLSDSV---------------- 60

Query: 61  PDTKKFNHSFIKLGWNSCISDTRLSLHFAAVHIARGFCGVSENGHSEKCLNDNASDGDVV 120
                    F+K GWN+CIS+TRL L  AAVHIARGFCGV ENG SEKCLND+ASDG VV
Sbjct: 61  ---------FVKSGWNTCISNTRLLLPLAAVHIARGFCGVPENGLSEKCLNDSASDGKVV 120

Query: 121 ANKVQGVHRNSVDFTKIPINMLPTVVLVGRPNVGKSALFNRFIRRREALVYNTPDDHVTR 180
           A KV+GV RNSVDFTKIPINMLPTVVLVGRPNVGKSALFNRFI+RREALVYNTPDDHVTR
Sbjct: 121 AKKVRGVQRNSVDFTKIPINMLPTVVLVGRPNVGKSALFNRFIKRREALVYNTPDDHVTR 180

Query: 181 DIRE------ELVFT--------------------------------------------- 240
           DIRE      +L F                                              
Sbjct: 181 DIREGVAKLGDLRFVVLDSSGLETAASSGSILERTARMTENVLLKSQLAIFLIDARAGLH 240

Query: 241 PLIWRLGSGLHAETCTDINIVVAMNKS-IVFDNDVHTFLVSLLSEAQRLGFGDPVPISAE 300
           P    +G  L   T  DIN+VVAMNKS  +FDN     L++   EAQRLGFGDPVPISAE
Sbjct: 241 PFDLEVGKWLRRHT-PDINLVVAMNKSESLFDNS--CTLLAAGFEAQRLGFGDPVPISAE 300

Query: 301 TGLGMQDLYWAIKPVLEKYMLKVINDNGGLDRFHLVTSSNEEEDTQDSKVPLQLAIVGRP 360
           TGLGMQ+LYWAIKPVLEKYMLKVI+DNGGLD FH VTSSNE+EDTQDSK  LQLAIVGRP
Sbjct: 301 TGLGMQELYWAIKPVLEKYMLKVISDNGGLDCFHQVTSSNEDEDTQDSKTKLQLAIVGRP 360

Query: 361 NVGKSTLLNTLLQSDRVLVGPEAGLTRDSVRAQFEFEGRTIYLVDTAGWLHRTKEEKGPG 420
           NVGKSTLLNTLLQS+RVLVGPEAGLTRDS+RAQFEFEGRTIYLVDTAGWLHRTKEEKGPG
Sbjct: 361 NVGKSTLLNTLLQSNRVLVGPEAGLTRDSIRAQFEFEGRTIYLVDTAGWLHRTKEEKGPG 420

Query: 421 SLSVMQSSKNLMRAHVVALVLDAEE--RDSNFLLRK----SRRLLVEVETLTWLLNRHPC 480
           SLSV+QS KNLMRAHVVALVLDAEE  R+   +       +RR + E   L  ++N+   
Sbjct: 421 SLSVVQSKKNLMRAHVVALVLDAEEIARERRSMKHSEVVIARRAVEEGRALVVIVNKMDL 480

Query: 481 NR----------------------------IRFIHFKDLKEEADFAIMRRVIETCEKWCS 524
            R                            I  I    L+      +M +VIET EKWC+
Sbjct: 481 MRGKKNFASYEKILEAVPEEIQTVIPQVTGIPVIFISALEGRGRLEVMHQVIETYEKWCT 540

BLAST of Tan0008543 vs. ExPASy TrEMBL
Match: A0A5A7V421 (GTP-binding protein EngA OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold22G003240 PE=3 SV=1)

HSP 1 Score: 546.6 bits (1407), Expect = 1.1e-151
Identity = 343/654 (52.45%), Postives = 388/654 (59.33%), Query Frame = 0

Query: 1   MSRLWVPLVFQPRGSSATTFSISVEQVERTSSNTLSLNFLSGSVLCNEGHMPSNFSTIPK 60
           MSRLW PL+ Q R          VE   R  S+T S N LS SV                
Sbjct: 1   MSRLWAPLLSQRRRFIGNGVFHFVETSRRICSSTPSPNLLSDSV---------------- 60

Query: 61  PDTKKFNHSFIKLGWNSCISDTRLSLHFAAVHIARGFCGVSENGHSEKCLNDNASDGDVV 120
                    F+K GWN+CIS+TRL L  AAVHIARGFCGV ENG SEKCLND+ASDG VV
Sbjct: 61  ---------FVKSGWNTCISNTRLLLPLAAVHIARGFCGVPENGLSEKCLNDSASDGKVV 120

Query: 121 ANKVQGVHRNSVDFTKIPINMLPTVVLVGRPNVGKSALFNRFIRRREALVYNTPDDHVTR 180
           A KV+GV RNSVDFTKIPINMLPTVVLVGRPNVGKSALFNRFI+RREALVYNTPDDHVTR
Sbjct: 121 AKKVRGVQRNSVDFTKIPINMLPTVVLVGRPNVGKSALFNRFIKRREALVYNTPDDHVTR 180

Query: 181 DIRE------ELVFT--------------------------------------------- 240
           DIRE      +L F                                              
Sbjct: 181 DIREGVAKLGDLRFVVLDSSGLETAASSGSILERTARMTENVLLKSQLAIFLIDARAGLH 240

Query: 241 PLIWRLGSGLHAETCTDINIVVAMNKS-IVFDNDVHTFLVSLLSEAQRLGFGDPVPISAE 300
           P    +G  L   T  DIN+VVAMNKS  +FDN        L  EAQRLGFGDPVPISAE
Sbjct: 241 PFDLEVGKWLRRHT-PDINLVVAMNKSESLFDNSCTLLAAGL--EAQRLGFGDPVPISAE 300

Query: 301 TGLGMQDLYWAIKPVLEKYMLKVINDNGGLDRFHLVTSSNEEEDTQDSKVPLQLAIVGRP 360
           TGLGMQ+LYWAIKPVLEKYMLKVI+DNGGLD FH VTSSNE+EDTQDSK  LQLAIVGRP
Sbjct: 301 TGLGMQELYWAIKPVLEKYMLKVISDNGGLDCFHQVTSSNEDEDTQDSKTKLQLAIVGRP 360

Query: 361 NVGKSTLLNTLLQSDRVLVGPEAGLTRDSVRAQFEFEGRTIYLVDTAGWLHRTKEEKGPG 420
           NVGKSTLLNTLLQS+RVLVGPEAGLTRDS+RAQFEFEGRTIYLVDTAGWLHRTKEEKGPG
Sbjct: 361 NVGKSTLLNTLLQSNRVLVGPEAGLTRDSIRAQFEFEGRTIYLVDTAGWLHRTKEEKGPG 420

Query: 421 SLSVMQSSKNLMRAHVVALVLDAEE--RDSNFLLRK----SRRLLVEVETLTWLLNRHPC 480
           SLSV+QS KNLMRAHVVALVLDAEE  R+   +       +RR + E   L  ++N+   
Sbjct: 421 SLSVVQSKKNLMRAHVVALVLDAEEIARERRSMKHSEVVIARRAVEEGRALVVIVNKMDL 480

Query: 481 NR----------------------------IRFIHFKDLKEEADFAIMRRVIETCEKWCS 524
            R                            I  I    L+      +M +VIET EKWC+
Sbjct: 481 MRGKKNFASYEKILEAVPEEIQTVIPQVTGIPVIFISALEGRGRLEVMHQVIETYEKWCT 540

BLAST of Tan0008543 vs. TAIR 10
Match: AT5G39960.1 (GTP binding;GTP binding )

HSP 1 Score: 335.9 bits (860), Expect = 5.9e-92
Identity = 207/489 (42.33%), Postives = 272/489 (55.62%), Query Frame = 0

Query: 69  SFIKLGWNSCISDTRLSLHFAAVHIARGFCGVSENGHSEKCLNDNASDGDVVANKVQGV- 128
           +F  LG+NS +        F +  I+      S NG +      N  +G+ + +K   + 
Sbjct: 43  NFSSLGFNSTVRHRSHQNRFCSNLISDLNLVRSTNGFATVSDESNVDNGEKLVDKKPKIF 102

Query: 129 HRNSVDFTKIPINMLPTVVLVGRPNVGKSALFNRFIRRREALVYNTPDDHVTRDIREELV 188
           ++  +DFTKI  N+LPTV+L+GRPNVGKSAL+NR IRRREALVYNTPDDHVTRDIRE + 
Sbjct: 103 YKKPIDFTKIDANLLPTVMLMGRPNVGKSALYNRLIRRREALVYNTPDDHVTRDIREGIA 162

Query: 189 ----------------------------------------FTPLIWRLGSGLH------- 248
                                                   F  LI  + +GLH       
Sbjct: 163 KLGDLRFNVLDSAGIETEVSSGTILGRTTAMTANVLARTQFAVLIIDVRAGLHPLDLEVG 222

Query: 249 ---AETCTDINIVVAMNKSIVFDNDVHTFLVSLLSEAQRLGFGDPVPISAETGLGMQDLY 308
               +    I  +V MNKS    +     L  + SEA  LGFG+P+ ISAETGLGM  LY
Sbjct: 223 KWLRKHAPQIKPIVVMNKSESIGS-----LDEVASEALALGFGEPIAISAETGLGMTTLY 282

Query: 309 WAIKPVLEKYMLKVINDNGGLDRFHLVTSSNEEEDTQDSKVPLQLAIVGRPNVGKSTLLN 368
             ++P+LE Y ++++ND G  D   ++T  N  ++  +SK+PLQLAIVG+PNVGKSTLLN
Sbjct: 283 EVLRPLLEDYTVEMLNDIGSQD--DVLTDENLSDEIDESKLPLQLAIVGKPNVGKSTLLN 342

Query: 369 TLLQSDRVLVGPEAGLTRDSVRAQFEFEGRTIYLVDTAGWLHRTKEEKGPGSLSVMQSSK 428
            LL+ +RVLVGPEAGLTRD+VR QFEF+GRT+YLVDTAGWL RT+ +KGP SLS+MQS K
Sbjct: 343 ALLEEERVLVGPEAGLTRDAVRVQFEFQGRTVYLVDTAGWLERTERDKGPASLSIMQSRK 402

Query: 429 NLMRAHVVALVLDAEE--------RDSNFLL-----RKSRRLLVEVETLTWLLNRH---- 473
           +LMRAHV+ALVLDAEE          S  ++      + R L+V V  +  L  R     
Sbjct: 403 SLMRAHVIALVLDAEEIIKAKCSMTHSEVVIARRAVEEGRGLVVIVNKMDRLRGRENSEM 462

BLAST of Tan0008543 vs. TAIR 10
Match: AT3G12080.1 (GTP-binding family protein )

HSP 1 Score: 102.8 bits (255), Expect = 8.4e-22
Identity = 101/360 (28.06%), Postives = 159/360 (44.17%), Query Frame = 0

Query: 110 LNDNASDGDVVANKVQGVHRNSVDFTKIPINMLPTVVLVGRPNVGKSALFNRFIRRREAL 169
           + D   +G     K + + +N+    +IP ++L  V +VGRPNVGKSALFNR +    A+
Sbjct: 129 IEDETIEGKETRRKGKRLAKNT---QQIPEHLLQRVAIVGRPNVGKSALFNRLVGENRAI 188

Query: 170 VYNTPDDHVTRD------------------------------IREELVFT--------PL 229
           V + P   VTRD                              + EEL  +        PL
Sbjct: 189 VVDEPG--VTRDRLYGRSYWGDQEFVVVDTGGVMTVSKSPSGVMEELNVSTTIGMEGIPL 248

Query: 230 IWRLGSGLHAETCTDINIVVAMNKS--IVFDND--------------------VHTFLVS 289
             R  +     +  +     A+++S  I+F  D                     H +++ 
Sbjct: 249 SSREAAIARMPSMIEKQATAAVDESAVIIFVVDGQAGPSGADVEIADWLRKYYSHKYIIL 308

Query: 290 LL-------------SEAQRLGFGDPVPISAETGLGMQDLYWAIKPVLEKYMLKVINDNG 349
            +             SE   LGF  P+PISA +G G  +L   +   L K          
Sbjct: 309 AVNKCESPRKGLMQASEFWSLGF-TPIPISALSGTGTGELLDLVCSGLIK---------- 368

Query: 350 GLDRFHLVTSSNEEEDTQDSKVPLQLAIVGRPNVGKSTLLNTLLQSDRVLVGPEAGLTRD 392
                 L    N EE+ +++ +P  +AI+GRPNVGKS++LN L++ DR +V P +G TRD
Sbjct: 369 ------LEIMENIEEEEEENYIP-AIAIIGRPNVGKSSILNALVREDRTIVSPVSGTTRD 428

BLAST of Tan0008543 vs. TAIR 10
Match: AT3G12080.2 (GTP-binding family protein )

HSP 1 Score: 102.8 bits (255), Expect = 8.4e-22
Identity = 101/360 (28.06%), Postives = 159/360 (44.17%), Query Frame = 0

Query: 110 LNDNASDGDVVANKVQGVHRNSVDFTKIPINMLPTVVLVGRPNVGKSALFNRFIRRREAL 169
           + D   +G     K + + +N+    +IP ++L  V +VGRPNVGKSALFNR +    A+
Sbjct: 129 IEDETIEGKETRRKGKRLAKNT---QQIPEHLLQRVAIVGRPNVGKSALFNRLVGENRAI 188

Query: 170 VYNTPDDHVTRD------------------------------IREELVFT--------PL 229
           V + P   VTRD                              + EEL  +        PL
Sbjct: 189 VVDEPG--VTRDRLYGRSYWGDQEFVVVDTGGVMTVSKSPSGVMEELNVSTTIGMEGIPL 248

Query: 230 IWRLGSGLHAETCTDINIVVAMNKS--IVFDND--------------------VHTFLVS 289
             R  +     +  +     A+++S  I+F  D                     H +++ 
Sbjct: 249 SSREAAIARMPSMIEKQATAAVDESAVIIFVVDGQAGPSGADVEIADWLRKYYSHKYIIL 308

Query: 290 LL-------------SEAQRLGFGDPVPISAETGLGMQDLYWAIKPVLEKYMLKVINDNG 349
            +             SE   LGF  P+PISA +G G  +L   +   L K          
Sbjct: 309 AVNKCESPRKGLMQASEFWSLGF-TPIPISALSGTGTGELLDLVCSGLIK---------- 368

Query: 350 GLDRFHLVTSSNEEEDTQDSKVPLQLAIVGRPNVGKSTLLNTLLQSDRVLVGPEAGLTRD 392
                 L    N EE+ +++ +P  +AI+GRPNVGKS++LN L++ DR +V P +G TRD
Sbjct: 369 ------LEIMENIEEEEEENYIP-AIAIIGRPNVGKSSILNALVREDRTIVSPVSGTTRD 428

BLAST of Tan0008543 vs. TAIR 10
Match: AT1G78010.1 (tRNA modification GTPase, putative )

HSP 1 Score: 63.5 bits (153), Expect = 5.7e-10
Identity = 46/108 (42.59%), Postives = 60/108 (55.56%), Query Frame = 0

Query: 300 LQLAIVGRPNVGKSTLLNTLLQSDRVLVGPEAGLTRDSVRAQFEFEGRTIYLVDTAGWLH 359
           LQ+AIVGRPNVGKS+LLN   +S+R +V   AG TRD V A     G  I L+DTAG + 
Sbjct: 318 LQIAIVGRPNVGKSSLLNAWSKSERAIVTEVAGTTRDVVEANVTVRGVPITLLDTAG-IR 377

Query: 360 RTKE--EKGPGSLSVMQSSKNLMRAHVVALVLDAEE---RDSNFLLRK 403
            T +  EK    + V +S      A V+ + + A E    +   LLRK
Sbjct: 378 ETNDIVEK----IGVERSETAAKVADVIIMAVSAVEGWTEEDTELLRK 420

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q1MDD61.4e-3732.43GTPase Der OS=Rhizobium leguminosarum bv. viciae (strain 3841) OX=216596 GN=der ... [more]
B0CK661.8e-3731.93GTPase Der OS=Brucella suis (strain ATCC 23445 / NCTC 10510) OX=470137 GN=der PE... [more]
Q2K5L21.8e-3733.09GTPase Der OS=Rhizobium etli (strain CFN 42 / ATCC 51251) OX=347834 GN=der PE=3 ... [more]
A6WW652.4e-3732.52GTPase Der OS=Ochrobactrum anthropi (strain ATCC 49188 / DSM 6882 / JCM 21032 / ... [more]
A9M8F44.1e-3732.02GTPase Der OS=Brucella canis (strain ATCC 23365 / NCTC 10854) OX=483179 GN=der P... [more]
Match NameE-valueIdentityDescription
XP_022984952.18.4e-17356.86uncharacterized protein LOC111483067 [Cucurbita maxima][more]
XP_023552191.17.1e-17256.71uncharacterized protein LOC111809943 [Cucurbita pepo subsp. pepo][more]
KAG6577347.14.6e-17156.40hypothetical protein SDJN03_24921, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022929494.11.0e-17056.25uncharacterized protein LOC111436046 [Cucurbita moschata][more]
XP_022136758.12.6e-16654.79uncharacterized protein LOC111008384 [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A6J1J3J04.1e-17356.86GTP-binding protein EngA OS=Cucurbita maxima OX=3661 GN=LOC111483067 PE=3 SV=1[more]
A0A6J1ENX05.0e-17156.25GTP-binding protein EngA OS=Cucurbita moschata OX=3662 GN=LOC111436046 PE=3 SV=1[more]
A0A6J1C8F61.3e-16654.79GTP-binding protein EngA OS=Momordica charantia OX=3673 GN=LOC111008384 PE=3 SV=... [more]
A0A1S3BT836.7e-15252.60GTP-binding protein EngA OS=Cucumis melo OX=3656 GN=LOC103493287 PE=3 SV=1[more]
A0A5A7V4211.1e-15152.45GTP-binding protein EngA OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol... [more]
Match NameE-valueIdentityDescription
AT5G39960.15.9e-9242.33GTP binding;GTP binding [more]
AT3G12080.18.4e-2228.06GTP-binding family protein [more]
AT3G12080.28.4e-2228.06GTP-binding family protein [more]
AT1G78010.15.7e-1042.59tRNA modification GTPase, putative [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006073GTP binding domainPRINTSPR00326GTP1OBGcoord: 302..322
score: 50.33
coord: 351..366
score: 27.8
IPR006073GTP binding domainPFAMPF01926MMR_HSR1coord: 145..186
e-value: 5.4E-8
score: 32.9
coord: 302..393
e-value: 1.5E-19
score: 70.1
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 120..198
e-value: 8.6E-10
score: 40.2
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 295..503
e-value: 1.1E-26
score: 95.3
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 228..398
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 141..268
IPR005225Small GTP-binding protein domainTIGRFAMTIGR00231TIGR00231coord: 301..394
e-value: 2.8E-14
score: 51.2
NoneNo IPR availablePANTHERPTHR43834GTPASE DERcoord: 127..188
coord: 207..474

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0008543.1Tan0008543.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009987 cellular process
molecular_function GO:0005525 GTP binding