Tan0008470 (gene) Snake gourd v1

Overview
NameTan0008470
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionprotein RALF-like 10
LocationLG09: 43357335 .. 43357550 (-)
RNA-Seq ExpressionTan0008470
SyntenyTan0008470
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGCAGTCATGGCTCATTTGTTTAGTTCTTATTAGCATGTTGGTGACAGGAAAAGAAGTAGCAGCGGACAGAACTTATCTTAAACTAGTGGATTGCAACAACCCAAACCCACCACCAAGTTGTTCTTCACCGAATGGAGAAGGTTCTACACCTGTTTCTGCTAATGCCTATACTCGAGGTTGCTCTGCCATCAATCGTTGTCGTGGAGGATGA

mRNA sequence

ATGAAGCAGTCATGGCTCATTTGTTTAGTTCTTATTAGCATGTTGGTGACAGGAAAAGAAGTAGCAGCGGACAGAACTTATCTTAAACTAGTGGATTGCAACAACCCAAACCCACCACCAAGTTGTTCTTCACCGAATGGAGAAGGTTCTACACCTGTTTCTGCTAATGCCTATACTCGAGGTTGCTCTGCCATCAATCGTTGTCGTGGAGGATGA

Coding sequence (CDS)

ATGAAGCAGTCATGGCTCATTTGTTTAGTTCTTATTAGCATGTTGGTGACAGGAAAAGAAGTAGCAGCGGACAGAACTTATCTTAAACTAGTGGATTGCAACAACCCAAACCCACCACCAAGTTGTTCTTCACCGAATGGAGAAGGTTCTACACCTGTTTCTGCTAATGCCTATACTCGAGGTTGCTCTGCCATCAATCGTTGTCGTGGAGGATGA

Protein sequence

MKQSWLICLVLISMLVTGKEVAADRTYLKLVDCNNPNPPPSCSSPNGEGSTPVSANAYTRGCSAINRCRGG
Homology
BLAST of Tan0008470 vs. ExPASy Swiss-Prot
Match: O64466 (Protein RALF-like 11 OS=Arabidopsis thaliana OX=3702 GN=RALFL11 PE=3 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 9.1e-09
Identity = 30/70 (42.86%), Postives = 42/70 (60.00%), Query Frame = 0

Query: 3  QSWLICLVLISMLVTGKEVAADRTYLK---LVDCNNPNPPPSCSSPNGEGSTPVSANAYT 62
          ++WLICL++I   V  + V + R Y++   +  C  PNPPP C+ P  E   P   N Y+
Sbjct: 2  KAWLICLLVICAAVIAEPVES-RNYIEYGAINKCAGPNPPPGCNPPGAEQKNPTPVNEYS 61

Query: 63 RGCSAINRCR 70
          RGCS I+RCR
Sbjct: 62 RGCSKIHRCR 70

BLAST of Tan0008470 vs. ExPASy Swiss-Prot
Match: F4ISE2 (Protein RALF-like 13 OS=Arabidopsis thaliana OX=3702 GN=RALFL13 PE=3 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 4.5e-08
Identity = 29/70 (41.43%), Postives = 42/70 (60.00%), Query Frame = 0

Query: 3  QSWLICLVLISMLVTGKEVAADRTYLK---LVDCNNPNPPPSCSSPNGEGSTPVSANAYT 62
          ++W+ICL++I   V  + V + R Y++   +  C  PNPPP C+ P  E   P   N Y+
Sbjct: 2  KAWVICLLVICAAVIAEPVES-RNYIEYGAINKCAGPNPPPGCNPPGAEQKNPNPVNEYS 61

Query: 63 RGCSAINRCR 70
          RGCS I+RCR
Sbjct: 62 RGCSKIHRCR 70

BLAST of Tan0008470 vs. ExPASy Swiss-Prot
Match: Q9LSG0 (Protein RALF-like 25 OS=Arabidopsis thaliana OX=3702 GN=RALFL25 PE=3 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 3.8e-07
Identity = 33/71 (46.48%), Postives = 41/71 (57.75%), Query Frame = 0

Query: 1  MKQSWLICLVLISMLVTGKEVAAD--RTYLKLVDCNNPNPPPSCSSPNGEGSTPVSANAY 60
          MK   +I LV+ S+L+ G+  A D  R YL L  C  PN PP C     +  TPV  N Y
Sbjct: 1  MKTFMIILLVICSILIVGRVEANDNKRKYLLLDPCLRPNAPPGCHRQPYKPRTPV--NVY 60

Query: 61 TRGCSAINRCR 70
          +RGC+ INRCR
Sbjct: 61 SRGCTTINRCR 69

BLAST of Tan0008470 vs. ExPASy Swiss-Prot
Match: F4ISE1 (Protein RALF-like 12 OS=Arabidopsis thaliana OX=3702 GN=RALFL12 PE=2 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 5.0e-07
Identity = 28/70 (40.00%), Postives = 42/70 (60.00%), Query Frame = 0

Query: 3  QSWLICLVLISMLVTGKEVAADRTYLK---LVDCNNPNPPPSCSSPNGEGSTPVSANAYT 62
          ++W+I L++I  +V  + V + R Y++   +  C  PNPPP C+ P  E   P   N Y+
Sbjct: 2  KAWVIGLLVICAVVIAEPVES-RNYIEYGAINKCAGPNPPPGCNPPGTEQKNPTPVNEYS 61

Query: 63 RGCSAINRCR 70
          RGCS I+RCR
Sbjct: 62 RGCSKIHRCR 70

BLAST of Tan0008470 vs. ExPASy Swiss-Prot
Match: O65919 (Protein RALF-like 10 OS=Arabidopsis thaliana OX=3702 GN=RALFL10 PE=2 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 2.5e-06
Identity = 27/67 (40.30%), Postives = 35/67 (52.24%), Query Frame = 0

Query: 6  LICLVLISMLVTGKEVAADRTYLK---LVDCNNPNPPPSCSSPNGEGSTPVSANAYTRGC 65
          +ICL++I   V    V + R +L    +  C  PNPPP C  P  +   P  AN Y RGC
Sbjct: 5  VICLLVIFAAVIAVPVESRRKHLDYGVITKCAGPNPPPGCYPPGAQQKNPTPANEYRRGC 64

Query: 66 SAINRCR 70
          S I RC+
Sbjct: 65 SKITRCK 71

BLAST of Tan0008470 vs. NCBI nr
Match: XP_038877580.1 (protein RALF-like 11 [Benincasa hispida])

HSP 1 Score: 84.0 bits (206), Expect = 5.9e-13
Identity = 43/70 (61.43%), Postives = 51/70 (72.86%), Query Frame = 0

Query: 3  QSWLICLVLISMLVTGKEVAADRTYLKLVD-CNNPNPPPSCSSPNGEGSTPVSANAYTRG 62
          +SWLIC+V ISMLV G  VAA++ YL+ +D CN PNPPP+CS    E    V AN Y RG
Sbjct: 2  KSWLICVVFISMLVVGPGVAAEKKYLQFLDPCNQPNPPPTCS----EVKEQVEANPYNRG 61

Query: 63 CSAINRCRGG 72
          CSAI+RCRGG
Sbjct: 62 CSAIHRCRGG 67

BLAST of Tan0008470 vs. NCBI nr
Match: XP_022947821.1 (protein RALF-like 10 [Cucurbita moschata])

HSP 1 Score: 75.9 bits (185), Expect = 1.6e-10
Identity = 40/70 (57.14%), Postives = 46/70 (65.71%), Query Frame = 0

Query: 3  QSWLICLVLISMLVTGKEVAADRTYLKLVD-CNNPNPPPSCSSPNGEGSTPVSANAYTRG 62
          +SWLICLVLI MLV  ++VA++  +LK  D C  PNPPP CS  N      V AN Y RG
Sbjct: 2  RSWLICLVLIGMLVVSQDVASETKFLKFPDPCQQPNPPPGCSIGNA-----VPANPYRRG 61

Query: 63 CSAINRCRGG 72
          CS I RCRGG
Sbjct: 62 CSKITRCRGG 66

BLAST of Tan0008470 vs. NCBI nr
Match: XP_023533089.1 (protein RALF-like 10 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 73.9 bits (180), Expect = 6.1e-10
Identity = 40/70 (57.14%), Postives = 46/70 (65.71%), Query Frame = 0

Query: 3  QSWLICLVLISMLVTGKEVAADRTYLKLVD-CNNPNPPPSCSSPNGEGSTPVSANAYTRG 62
          +SWLIC+VLISMLV  +EVA++  +LK  D C  PNPP  CS  N      V AN Y RG
Sbjct: 2  RSWLICIVLISMLVVSQEVASETKFLKFPDPCQQPNPPLGCSIGNA-----VPANPYRRG 61

Query: 63 CSAINRCRGG 72
          CS I RCRGG
Sbjct: 62 CSKITRCRGG 66

BLAST of Tan0008470 vs. NCBI nr
Match: KAE8637268.1 (hypothetical protein CSA_017684 [Cucumis sativus])

HSP 1 Score: 68.2 bits (165), Expect = 3.3e-08
Identity = 37/69 (53.62%), Postives = 46/69 (66.67%), Query Frame = 0

Query: 3  QSWLICLVLISMLVTGKEVAADRTYLKLVD-CNNPNPPPSCSSPNGEGSTPVSANAYTRG 62
          +SWLIC+V ISMLV  +EV A   +LK  + C+ P+PPPSCSS   +       N Y RG
Sbjct: 2  KSWLICVVFISMLVMSQEVGAQTNHLKFPNPCDLPSPPPSCSS---KVKDHAPTNPYDRG 61

Query: 63 CSAINRCRG 71
          CSAI+RCRG
Sbjct: 62 CSAIHRCRG 67

BLAST of Tan0008470 vs. NCBI nr
Match: KAG7020863.1 (hypothetical protein SDJN02_17551, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 66.6 bits (161), Expect = 9.7e-08
Identity = 35/71 (49.30%), Postives = 47/71 (66.20%), Query Frame = 0

Query: 7  ICLVLISMLVTGKEVAADR------TYLKLVDCNNPNPPPSCSSPNGEGSTPVSANAYTR 66
          ICL LI+ML+T ++V  ++       YLK ++C++PNPP  CS    +G   V AN Y R
Sbjct: 6  ICLALITMLMTSQQVMTNQEATSQIKYLKHLECHHPNPPAHCSLSGVKGYQHVPANPYDR 65

Query: 67 GCSAINRCRGG 72
          GCSAI+RCRGG
Sbjct: 66 GCSAIHRCRGG 76

BLAST of Tan0008470 vs. ExPASy TrEMBL
Match: A0A0A0LI94 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G191310 PE=3 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 9.1e-12
Identity = 42/70 (60.00%), Postives = 50/70 (71.43%), Query Frame = 0

Query: 3  QSWLICLVLISMLVTGKEVAADRTYLKLVD-CNNPNPPPSCSSPNGEGSTPVSANAYTRG 62
          +SWLICL LI MLV G+EVAA+  +LK +D CN P+PPP+C  PN +      AN Y R 
Sbjct: 2  KSWLICLALIGMLVVGQEVAAETKHLKFIDPCNQPSPPPTC--PNRD--KVEEANPYNRD 61

Query: 63 CSAINRCRGG 72
          CSAINRCRGG
Sbjct: 62 CSAINRCRGG 67

BLAST of Tan0008470 vs. ExPASy TrEMBL
Match: A0A6J1G7J2 (protein RALF-like 10 OS=Cucurbita moschata OX=3662 GN=LOC111451581 PE=3 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 7.7e-11
Identity = 40/70 (57.14%), Postives = 46/70 (65.71%), Query Frame = 0

Query: 3  QSWLICLVLISMLVTGKEVAADRTYLKLVD-CNNPNPPPSCSSPNGEGSTPVSANAYTRG 62
          +SWLICLVLI MLV  ++VA++  +LK  D C  PNPPP CS  N      V AN Y RG
Sbjct: 2  RSWLICLVLIGMLVVSQDVASETKFLKFPDPCQQPNPPPGCSIGNA-----VPANPYRRG 61

Query: 63 CSAINRCRGG 72
          CS I RCRGG
Sbjct: 62 CSKITRCRGG 66

BLAST of Tan0008470 vs. ExPASy TrEMBL
Match: A0A0A0M0X3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G690160 PE=3 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.6e-08
Identity = 37/69 (53.62%), Postives = 46/69 (66.67%), Query Frame = 0

Query: 3  QSWLICLVLISMLVTGKEVAADRTYLKLVD-CNNPNPPPSCSSPNGEGSTPVSANAYTRG 62
          +SWLIC+V ISMLV  +EV A   +LK  + C+ P+PPPSCSS   +       N Y RG
Sbjct: 2  KSWLICVVFISMLVMSQEVGAQTNHLKFPNPCDLPSPPPSCSS---KVKDHAPTNPYDRG 61

Query: 63 CSAINRCRG 71
          CSAI+RCRG
Sbjct: 62 CSAIHRCRG 67

BLAST of Tan0008470 vs. ExPASy TrEMBL
Match: A0A1S4DY33 (protein RALF-like 30 OS=Cucumis melo OX=3656 GN=LOC107991083 PE=3 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 3.0e-07
Identity = 37/70 (52.86%), Postives = 45/70 (64.29%), Query Frame = 0

Query: 3  QSWLICLVLISMLVTGKEVAADRTYLKLVD-CNNPNPPPSCSSPNGEGSTPVSANAYTRG 62
          +SWLIC  L+SMLV G EVAA   +LK +D C  P+PP SC   +    +P+   AY RG
Sbjct: 2  KSWLICFALLSMLVMGHEVAAKTKFLKFLDPCLQPSPPRSCYK-HERVDSPI---AYDRG 61

Query: 63 CSAINRCRGG 72
          CS I RCRGG
Sbjct: 62 CSPILRCRGG 67

BLAST of Tan0008470 vs. ExPASy TrEMBL
Match: B2Y4P6 (Putative rapid alkalinization factor (RALF) family protein OS=Arabidopsis halleri subsp. halleri OX=81971 GN=7C17.3 PE=3 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 8.9e-07
Identity = 31/70 (44.29%), Postives = 43/70 (61.43%), Query Frame = 0

Query: 3  QSWLICLVLISMLVTGKEVAADRTYLK---LVDCNNPNPPPSCSSPNGEGSTPVSANAYT 62
          ++W+ICL++IS  VT + V + R Y++   +  C  PNPPP C  P  E   P   N Y+
Sbjct: 2  KAWVICLLVISAAVTAEPVES-RKYIEDGAINKCAGPNPPPGCHPPGAEDKKPTPVNEYS 61

Query: 63 RGCSAINRCR 70
          RGCS I+RCR
Sbjct: 62 RGCSKIHRCR 70

BLAST of Tan0008470 vs. TAIR 10
Match: AT2G19030.1 (ralf-like 11 )

HSP 1 Score: 60.5 bits (145), Expect = 6.5e-10
Identity = 30/70 (42.86%), Postives = 42/70 (60.00%), Query Frame = 0

Query: 3  QSWLICLVLISMLVTGKEVAADRTYLK---LVDCNNPNPPPSCSSPNGEGSTPVSANAYT 62
          ++WLICL++I   V  + V + R Y++   +  C  PNPPP C+ P  E   P   N Y+
Sbjct: 2  KAWLICLLVICAAVIAEPVES-RNYIEYGAINKCAGPNPPPGCNPPGAEQKNPTPVNEYS 61

Query: 63 RGCSAINRCR 70
          RGCS I+RCR
Sbjct: 62 RGCSKIHRCR 70

BLAST of Tan0008470 vs. TAIR 10
Match: AT2G19045.1 (RALF-like 13 )

HSP 1 Score: 58.2 bits (139), Expect = 3.2e-09
Identity = 29/70 (41.43%), Postives = 42/70 (60.00%), Query Frame = 0

Query: 3  QSWLICLVLISMLVTGKEVAADRTYLK---LVDCNNPNPPPSCSSPNGEGSTPVSANAYT 62
          ++W+ICL++I   V  + V + R Y++   +  C  PNPPP C+ P  E   P   N Y+
Sbjct: 2  KAWVICLLVICAAVIAEPVES-RNYIEYGAINKCAGPNPPPGCNPPGAEQKNPNPVNEYS 61

Query: 63 RGCSAINRCR 70
          RGCS I+RCR
Sbjct: 62 RGCSKIHRCR 70

BLAST of Tan0008470 vs. TAIR 10
Match: AT3G25165.1 (ralf-like 25 )

HSP 1 Score: 55.1 bits (131), Expect = 2.7e-08
Identity = 33/71 (46.48%), Postives = 41/71 (57.75%), Query Frame = 0

Query: 1  MKQSWLICLVLISMLVTGKEVAAD--RTYLKLVDCNNPNPPPSCSSPNGEGSTPVSANAY 60
          MK   +I LV+ S+L+ G+  A D  R YL L  C  PN PP C     +  TPV  N Y
Sbjct: 1  MKTFMIILLVICSILIVGRVEANDNKRKYLLLDPCLRPNAPPGCHRQPYKPRTPV--NVY 60

Query: 61 TRGCSAINRCR 70
          +RGC+ INRCR
Sbjct: 61 SRGCTTINRCR 69

BLAST of Tan0008470 vs. TAIR 10
Match: AT2G19040.1 (RALF-like 12 )

HSP 1 Score: 54.7 bits (130), Expect = 3.6e-08
Identity = 28/70 (40.00%), Postives = 42/70 (60.00%), Query Frame = 0

Query: 3  QSWLICLVLISMLVTGKEVAADRTYLK---LVDCNNPNPPPSCSSPNGEGSTPVSANAYT 62
          ++W+I L++I  +V  + V + R Y++   +  C  PNPPP C+ P  E   P   N Y+
Sbjct: 2  KAWVIGLLVICAVVIAEPVES-RNYIEYGAINKCAGPNPPPGCNPPGTEQKNPTPVNEYS 61

Query: 63 RGCSAINRCR 70
          RGCS I+RCR
Sbjct: 62 RGCSKIHRCR 70

BLAST of Tan0008470 vs. TAIR 10
Match: AT2G19020.1 (ralf-like 10 )

HSP 1 Score: 52.4 bits (124), Expect = 1.8e-07
Identity = 27/67 (40.30%), Postives = 35/67 (52.24%), Query Frame = 0

Query: 6  LICLVLISMLVTGKEVAADRTYLK---LVDCNNPNPPPSCSSPNGEGSTPVSANAYTRGC 65
          +ICL++I   V    V + R +L    +  C  PNPPP C  P  +   P  AN Y RGC
Sbjct: 5  VICLLVIFAAVIAVPVESRRKHLDYGVITKCAGPNPPPGCYPPGAQQKNPTPANEYRRGC 64

Query: 66 SAINRCR 70
          S I RC+
Sbjct: 65 SKITRCK 71

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O644669.1e-0942.86Protein RALF-like 11 OS=Arabidopsis thaliana OX=3702 GN=RALFL11 PE=3 SV=1[more]
F4ISE24.5e-0841.43Protein RALF-like 13 OS=Arabidopsis thaliana OX=3702 GN=RALFL13 PE=3 SV=1[more]
Q9LSG03.8e-0746.48Protein RALF-like 25 OS=Arabidopsis thaliana OX=3702 GN=RALFL25 PE=3 SV=1[more]
F4ISE15.0e-0740.00Protein RALF-like 12 OS=Arabidopsis thaliana OX=3702 GN=RALFL12 PE=2 SV=1[more]
O659192.5e-0640.30Protein RALF-like 10 OS=Arabidopsis thaliana OX=3702 GN=RALFL10 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_038877580.15.9e-1361.43protein RALF-like 11 [Benincasa hispida][more]
XP_022947821.11.6e-1057.14protein RALF-like 10 [Cucurbita moschata][more]
XP_023533089.16.1e-1057.14protein RALF-like 10 [Cucurbita pepo subsp. pepo][more]
KAE8637268.13.3e-0853.62hypothetical protein CSA_017684 [Cucumis sativus][more]
KAG7020863.19.7e-0849.30hypothetical protein SDJN02_17551, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
A0A0A0LI949.1e-1260.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G191310 PE=3 SV=1[more]
A0A6J1G7J27.7e-1157.14protein RALF-like 10 OS=Cucurbita moschata OX=3662 GN=LOC111451581 PE=3 SV=1[more]
A0A0A0M0X31.6e-0853.62Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G690160 PE=3 SV=1[more]
A0A1S4DY333.0e-0752.86protein RALF-like 30 OS=Cucumis melo OX=3656 GN=LOC107991083 PE=3 SV=1[more]
B2Y4P68.9e-0744.29Putative rapid alkalinization factor (RALF) family protein OS=Arabidopsis haller... [more]
Match NameE-valueIdentityDescription
AT2G19030.16.5e-1042.86ralf-like 11 [more]
AT2G19045.13.2e-0941.43RALF-like 13 [more]
AT3G25165.12.7e-0846.48ralf-like 25 [more]
AT2G19040.13.6e-0840.00RALF-like 12 [more]
AT2G19020.11.8e-0740.30ralf-like 10 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008801Rapid ALkalinization FactorPFAMPF05498RALFcoord: 31..70
e-value: 7.0E-9
score: 36.5
NoneNo IPR availablePANTHERPTHR34270:SF5PROTEIN RALF-LIKE 10-RELATEDcoord: 3..71
NoneNo IPR availablePANTHERPTHR34270PROTEIN RALF-LIKE 15-RELATEDcoord: 3..71

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0008470.1Tan0008470.1mRNA