Tan0008195 (gene) Snake gourd v1

Overview
NameTan0008195
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionnaringenin,2-oxoglutarate 3-dioxygenase
LocationLG01: 27668117 .. 27673192 (+)
RNA-Seq ExpressionTan0008195
SyntenyTan0008195
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCAACTAAATCCGCCATTTTTGCACTCTCCAAGAAAATCTTCCCCAAATCAAATCAATGGCTTCTTCCACTCTCACTGCTCTCGCCGACGAGCCGACACTGCAGGCCTCTTTCATCCGCGACGAGGACGAGCGTCCTAAAATCCCTTACAACCATTTCAGCGATGAAGTTCCTATCATCTCACTCAACGGAATCGACGACACCGCCGGTGGCCGCAGGGCTGAGATTTGTCGGAAAATCGTCGAGGCCTGCGAGGACTGGGGGATATTCCAGGTCGTCGACCATGGCGTCGATTCGGAGCTTATTTCCGAGATGACTCGTCTCGCTAGAGAGTTCTTTGCCCTTCCGCCGGAGGAGAAGCTACGTTTCGACATGTCCGGCGGTAAGAAGGGCGGATTCATCGTCTCCAGTCATCTCAAGGTGACGATATCGAAAACCCTCCTCTGATTTCTCCATTAGTCGATGATGTTTTACAGATCTGTGGATTTAATCTGTTCAGTTGCGATGTGTTATGGTTTTTTCTCTCTTTTTCTTTGGACCTGTAATTGTAAATGCTCATCAGTATTGAAATTGGGAGGACATTTATTACAGGCTCTACCGATAGATGATCATTATAAATGGTAGGGAGCCTACCTCAACTAATTAGCATTTAGGGTCTTTTTGGGTGAAAATTATAATAACTCGTAAAATACTGGGAACTCTACAAATGCAAACTATACTACATTTTAAATAATATTATATAAATTTCACCGATTATAATAACACACGTTATTTTCAATACTAAAAAGAATCCTCGATTGTTGAAATTTTTTATAATTTAAATGGAAAATTTTCTTAATACTTTTGTTTTTTAGTACTCTAACATTTGGTGTTAGAGGATTCAAATTTATTGACCTCTTAGCTTGGTCATAAATCTTACTTATACTTAAAGCTATATTCCTGTTGGCAATTTTCCATATCACTATTATTTATTATTCACCACATTGATATTCTTTTGAAAATGATACGAGGTCTACTAAAAATCATGTGGGATACACTGTTATTAAAGTATTGTGTGAACAAAAGAAGTGCATGTTAGAGAATGATTTTAGAAATATTGATTGTAGTGTAATTGATTTGTTTATAATTGATTTATTAAAAGTGATTTTCATATAATTATTTTTATAATTATTTTCATGTTTGGTTCTATACTTTTAGTGATTTTGGTTTTGAAAAAAAATCATTTTTTATGAAAATTTATGAATTTTGACAAAAGATTATAGTTTTTACTTGTCATTAAAAGTATGCTATTTTAAAAAATGAATAATAGTCATTTTAAATTACAATCATTTGACGAGAAAATCACTTCAAATGATTTTGGATTCTTCCAAAATCACTTGTTTAATATTGTCAAACATCACACTTTAAGTAGAAAAATGATTTTAGACATGTAAAAAGTGATTTTGGTCACTCGAAAATCACTCCAAAACATGTCTAAAGTATGATATTCTGTTAGTACTCAAAATTTTCGAAGAAATTTGACTGGGTGATCAAACTTACATATTAATTATGGTTGAGCTCACTTTTGCGGTTGATTTCAACCTCGTTAGATATAATGATTTAGGGGAAAATTAAATGATTATTGTTAAAGCCAGCAGTTCTCACTTATTGTTCTTAAAACGTGTGATTACTTAATGAGGGGCTGAATAATTTTTCTTATAATAAATAAGTCGATTGATTGGTGGAAAGCCCAAGTTTAGCTATCCTCTAATCCCGATGCTAGCTTTCAATTTCGATTCTTTTTTTTTTTTAGTATACTCTGTAAAAAAAATTTAATTCTAGGAGAGTATGATTAATATCGGTGGAACCCACCATTTTCAAAAATATGATGTAGTGACAAACCATTAACAGAGGTTGGCATCTGTTTGTGACGAATTGCTACTAGAGAAAGTATTTCTAGTACTATTATCATAATAGCATACTTTTTATGTTCACATTATGTTATAATATTAGTAAATCCCATATCATTTATGTTGGACCCGACATAATTATGAAAAAGTGGTAAATAAATGAAAGTAGTATGGAAAATTTTCTATATTATCCTAATACTAGAAATTTTCCCAATCTTGATGTAGGGTGTCATTTTCATCCTTTTAGAACTTTTACTATACCACTATTTTGATCCCTTAACTCTTAGCTATGGACCATTTCAGCCTACTAATTTAAAGTTTAGCACAAGGTTGAACACTTACTATTAACTAGATAGCTTTATATAGTGTTAAGTGGATAGATTATGGTATATTTTCAGGGGGAAGCGGTTAAAGATTGGCGTGAGATAGTGACCTACTTCGCATATCCGATCAGAAACAGAAACTACTCTCTATGGCCGGACAAGCCAGAGGGGTGGGCGGCGAAGACGGCGGAGTACAGCGAGAAGCTGATGGGGTTGGCCTGTAAGCTTTTGGAGGTTTTATCAGAAGCCATGGGGCTTGAGAAAGAGGCATTAACCAAGGCCTGTGTGGACATGGACCAGAAGATTGTGGTCAATTTCTATCCCAAATGCCCTCAGCCAGACCTCACTTTGGGGCTGAAGCGCCACACTGATCCTGGAACCATAACACTGCTGCTGCAGGACCAGGTTGGCGGACTTCAGGCTACCAAAGACGGTGGTCGGTCGTGGATCACCGTTCAGCCCGTCGAAGGAGCTTTCGTCGTCAACCTCGGAGACCATGGCCATGTAAGTGTGTGTTTGATATGTCTTTTGTAATGCTTATGAATGACTGAAACTAACTATTTTAAAAAATATTAGCGCCTGGTTGAAATTTTAGAGTAGTAAAAAATACTTTCGATCAACTAAAAATATTTTTTTTATTTTAAAATTGATTTTAGAAAGTTATTTTACCAATTAAAAATAGATCTAGAGATAATTAAGAAATGATTTTTGAAGAAGCATTTAAATAATTTTTATTTTTTTATAAATGTTTATATTACACTACTTCACCTAAAAATACTTCTTTTTAAAAGATATTGAACTGATCTCTTTAACAAATCTCTTTAAGCACTTTGAAAAATCTCTTTAAGCACTTTGAAAAATAAGAGAGAACATTGAGAATAAGGTTTAAAATGTGATGGTCTCAATTTTTTTGGAAATGTCGATAAAATGTTAATGTGATGATTTTTGAGAAATTTTAAAAATATATTTAAATTAAAAAATAAACATTTTAAATGCGTTAAAATACTTAATATAATAGGTATTTACCTGATGTTATATTTTGTTGTTATTTTTTTAATATTGCATCAATTTTATATGTTATAATAGAAATATCGATATCAAACCTATACATACGTAAAAAATATCGATGAAAATGTCAATATGTCAAATTTAATGATAGGATTGAGAGAGAAGAGAATTGACGACATGGTGGGAGATCTAAATAAAAATAAAGAAATTAATGTGATTTGACTTTTAACTTTTAAGTTTCTGGGGCTGCATAAAAAAAATTAGTTTAAAACAAATCTGCATAAATTTACCTGGGCCCACGGTTTTGAGGAGGAATAGAAAGCCACGTTGCTTTTCTTAAAATAATAAAAAAGAAATTCTCTTCTATTTATAGTTTTAAAAAATCAAGATTGAATTTTCTTCACTTTTTTACTAATATTTTAATCTGCGTAGTTTTCAAATATTCCTTTAGCTTACCTTAAAATTGAATCTTATTATTAACTTTCCTAAACAAAATTAACCGCTATTAAACTTCATTCTATAAATTTTGAAAATATGTTTACATCATGCATTTTCTGGTAAGAAAATTATTATTATTACTTAACCAATTTTGAGAAAAAATAACTTTAAAGTGATGATAGGACTCAATTTTAAACTTGATAAAAAGTATAAAAACTGAACATTTAAGAGTACCATAACAAAAATGGAATGAAATTCAAGTATTTTAACCTCTTTTAAAATATATTCAACACTTTCATTTTCTTCTTTTTCATCTCCTTTATTTTATTTTTACCTTTTTCCTTTTTCTCACTCTACTTTAAATTATTTTATACATCTTAAAAATAAAATAAGTAAGAATATTGATTTTTTTTAATTTTAATTACTATATTTCTTTATATTCGTAACTTTATTATTTTCAAATAATTAAATAATTATTTTTCCTCACTTCTATTAATATTATAATTAAATTTTGTTTACAATTATTTAAGATAGTTGTATCAGCTTCATTAAATAGTTTTTTTTATAGTAATTACCAATCAAAGCATTACTAAAAAAAAGTAAAATAAGCCAATAGTGGAAAGTAAAGGAATTAGGTGCAATCTACAAAGAAAACTTATCAGTATTATTTTTGGGTAAATTTAACCCCCTTCATTTATTGCTTTGTCACATCATCTTTTTAAGAATAATGTAGAAAACACATACAATAAATATACATACATATATATATATATATATACATACAAAATTACAATGTCAACAAAAGAAATAAGGTACGGTATACAGTATTGTAGTACTAGAAAATTTTCCTTCGAACTTAGACATGAATTAACGAGTTTATTGATAGTTGCAACAGTATCTTAGCAATGGTAGGTTCAAGAATGCTGATCATCAAGCAGTGGTGAATTCAAATACAAGTAGGCTTTCAATAGCCACATTTCAAAACCCAGCACCAGAAGCCATTGTTTATCCATTGAAGATCAGAGATGGAGAGAAGCCAATTCTTGAGAAACCCATCACTTTCACTGAGATGTATCAGAGCAAGATGAGCAGGGATCTTGAGCTTGCAAGGCTCAAGAAACTGGCCCATCAACAACATTCCTCTGAGTTATTGGATCAGGCCCAGCCCAATGGCCAAATCTTTGCTTGAAAACTCATTATCCCATCTTTCTCATCTCTTTGATCATGTGTGTTTTCTTTATTTTATCTTAATAGTTTTTTTATGGTGGGTAGTTTTGGGTTTAGTTTGTGCTCTATTGTATGGAATAAGTGTAATATGCATGGATCTATGAAATTCTCTAAACCTATTAACAATGTAATTCTAATGTTTAGATTGGGAGATTTGAATTGTGGTGGCTTTCTTTTAAGTTGGTTGTTAG

mRNA sequence

CCCAACTAAATCCGCCATTTTTGCACTCTCCAAGAAAATCTTCCCCAAATCAAATCAATGGCTTCTTCCACTCTCACTGCTCTCGCCGACGAGCCGACACTGCAGGCCTCTTTCATCCGCGACGAGGACGAGCGTCCTAAAATCCCTTACAACCATTTCAGCGATGAAGTTCCTATCATCTCACTCAACGGAATCGACGACACCGCCGGTGGCCGCAGGGCTGAGATTTGTCGGAAAATCGTCGAGGCCTGCGAGGACTGGGGGATATTCCAGGTCGTCGACCATGGCGTCGATTCGGAGCTTATTTCCGAGATGACTCGTCTCGCTAGAGAGTTCTTTGCCCTTCCGCCGGAGGAGAAGCTACGTTTCGACATGTCCGGCGGTAAGAAGGGCGGATTCATCGTCTCCAGTCATCTCAAGGGGGAAGCGGTTAAAGATTGGCGTGAGATAGTGACCTACTTCGCATATCCGATCAGAAACAGAAACTACTCTCTATGGCCGGACAAGCCAGAGGGGTGGGCGGCGAAGACGGCGGAGTACAGCGAGAAGCTGATGGGGTTGGCCTGTAAGCTTTTGGAGGTTTTATCAGAAGCCATGGGGCTTGAGAAAGAGGCATTAACCAAGGCCTGTGTGGACATGGACCAGAAGATTGTGGTCAATTTCTATCCCAAATGCCCTCAGCCAGACCTCACTTTGGGGCTGAAGCGCCACACTGATCCTGGAACCATAACACTGCTGCTGCAGGACCAGGTTGGCGGACTTCAGGCTACCAAAGACGGTGGTCGGTCGTGGATCACCGTTCAGCCCGTCGAAGGAGCTTTCGTCGTCAACCTCGGAGACCATGGCCATTATCTTAGCAATGGTAGGTTCAAGAATGCTGATCATCAAGCAGTGGTGAATTCAAATACAAGTAGGCTTTCAATAGCCACATTTCAAAACCCAGCACCAGAAGCCATTGTTTATCCATTGAAGATCAGAGATGGAGAGAAGCCAATTCTTGAGAAACCCATCACTTTCACTGAGATGTATCAGAGCAAGATGAGCAGGGATCTTGAGCTTGCAAGGCTCAAGAAACTGGCCCATCAACAACATTCCTCTGAGTTATTGGATCAGGCCCAGCCCAATGGCCAAATCTTTGCTTGAAAACTCATTATCCCATCTTTCTCATCTCTTTGATCATGTGTGTTTTCTTTATTTTATCTTAATAGTTTTTTTATGGTGGGTAGTTTTGGGTTTAGTTTGTGCTCTATTGTATGGAATAAGTGTAATATGCATGGATCTATGAAATTCTCTAAACCTATTAACAATGTAATTCTAATGTTTAGATTGGGAGATTTGAATTGTGGTGGCTTTCTTTTAAGTTGGTTGTTAG

Coding sequence (CDS)

ATGGCTTCTTCCACTCTCACTGCTCTCGCCGACGAGCCGACACTGCAGGCCTCTTTCATCCGCGACGAGGACGAGCGTCCTAAAATCCCTTACAACCATTTCAGCGATGAAGTTCCTATCATCTCACTCAACGGAATCGACGACACCGCCGGTGGCCGCAGGGCTGAGATTTGTCGGAAAATCGTCGAGGCCTGCGAGGACTGGGGGATATTCCAGGTCGTCGACCATGGCGTCGATTCGGAGCTTATTTCCGAGATGACTCGTCTCGCTAGAGAGTTCTTTGCCCTTCCGCCGGAGGAGAAGCTACGTTTCGACATGTCCGGCGGTAAGAAGGGCGGATTCATCGTCTCCAGTCATCTCAAGGGGGAAGCGGTTAAAGATTGGCGTGAGATAGTGACCTACTTCGCATATCCGATCAGAAACAGAAACTACTCTCTATGGCCGGACAAGCCAGAGGGGTGGGCGGCGAAGACGGCGGAGTACAGCGAGAAGCTGATGGGGTTGGCCTGTAAGCTTTTGGAGGTTTTATCAGAAGCCATGGGGCTTGAGAAAGAGGCATTAACCAAGGCCTGTGTGGACATGGACCAGAAGATTGTGGTCAATTTCTATCCCAAATGCCCTCAGCCAGACCTCACTTTGGGGCTGAAGCGCCACACTGATCCTGGAACCATAACACTGCTGCTGCAGGACCAGGTTGGCGGACTTCAGGCTACCAAAGACGGTGGTCGGTCGTGGATCACCGTTCAGCCCGTCGAAGGAGCTTTCGTCGTCAACCTCGGAGACCATGGCCATTATCTTAGCAATGGTAGGTTCAAGAATGCTGATCATCAAGCAGTGGTGAATTCAAATACAAGTAGGCTTTCAATAGCCACATTTCAAAACCCAGCACCAGAAGCCATTGTTTATCCATTGAAGATCAGAGATGGAGAGAAGCCAATTCTTGAGAAACCCATCACTTTCACTGAGATGTATCAGAGCAAGATGAGCAGGGATCTTGAGCTTGCAAGGCTCAAGAAACTGGCCCATCAACAACATTCCTCTGAGTTATTGGATCAGGCCCAGCCCAATGGCCAAATCTTTGCTTGA

Protein sequence

MASSTLTALADEPTLQASFIRDEDERPKIPYNHFSDEVPIISLNGIDDTAGGRRAEICRKIVEACEDWGIFQVVDHGVDSELISEMTRLAREFFALPPEEKLRFDMSGGKKGGFIVSSHLKGEAVKDWREIVTYFAYPIRNRNYSLWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAMGLEKEALTKACVDMDQKIVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDGGRSWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNTSRLSIATFQNPAPEAIVYPLKIRDGEKPILEKPITFTEMYQSKMSRDLELARLKKLAHQQHSSELLDQAQPNGQIFA
Homology
BLAST of Tan0008195 vs. ExPASy Swiss-Prot
Match: Q05965 (Naringenin,2-oxoglutarate 3-dioxygenase (Fragment) OS=Matthiola incana OX=3724 GN=FHT PE=2 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 7.3e-171
Identity = 288/360 (80.00%), Postives = 324/360 (90.00%), Query Frame = 0

Query: 2   ASSTLTALADEPTLQASFIRDEDERPKIPYNHFSDEVPIISLNGIDDTAGGRRAEICRKI 61
           A  TLT LA E  L + F+RDEDERPK+ YN FSDE+P+ISL GIDD   G+R EICR+I
Sbjct: 1   APGTLTELAGESKLNSKFVRDEDERPKVAYNEFSDEIPVISLAGIDD-VDGKRGEICREI 60

Query: 62  VEACEDWGIFQVVDHGVDSELISEMTRLAREFFALPPEEKLRFDMSGGKKGGFIVSSHLK 121
           VEACE+WGIFQVVDHGVD+ L+++MTRLAR+FFALPPEEKLRFDMSGGKKGGFIVSSHL+
Sbjct: 61  VEACENWGIFQVVDHGVDTSLVADMTRLARDFFALPPEEKLRFDMSGGKKGGFIVSSHLQ 120

Query: 122 GEAVKDWREIVTYFAYPIRNRNYSLWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAMG 181
           GEAV+DWREIVTYF+YP+RNR+YS WPDKP+GWA  T EYSEKLMGLACKLLEVLSEAMG
Sbjct: 121 GEAVQDWREIVTYFSYPVRNRDYSRWPDKPQGWAKVTEEYSEKLMGLACKLLEVLSEAMG 180

Query: 182 LEKEALTKACVDMDQKIVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDG 241
           LEKE+LT ACVDMDQKIVVN+YPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQAT+D 
Sbjct: 181 LEKESLTNACVDMDQKIVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRDD 240

Query: 242 GRSWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNTSRLSIATFQNPAPEAIV 301
           G +WITVQPVEGAFVVNLGDHGH+LSNGRFKNADHQAVVNSN+SRLSIATFQNPAPEA V
Sbjct: 241 GNTWITVQPVEGAFVVNLGDHGHFLSNGRFKNADHQAVVNSNSSRLSIATFQNPAPEATV 300

Query: 302 YPLKIRDGEKPILEKPITFTEMYQSKMSRDLELARLKKLAHQQHSSELLDQAQPNGQIFA 361
           YPLK+R+GEK I+E+PITF EMY+ KM RDLELARLKKLA ++H+ +  + A+P  QI A
Sbjct: 301 YPLKVREGEKAIMEEPITFAEMYKRKMGRDLELARLKKLAKEEHNHK--EAAKPLDQILA 357

BLAST of Tan0008195 vs. ExPASy Swiss-Prot
Match: Q07353 (Naringenin,2-oxoglutarate 3-dioxygenase (Fragment) OS=Petunia hybrida OX=4102 GN=AN3 PE=1 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 1.4e-169
Identity = 286/341 (83.87%), Postives = 316/341 (92.67%), Query Frame = 0

Query: 4   STLTALADEPTLQASFIRDEDERPKIPYNHFSDEVPIISLNGIDDTAGGRRAEICRKIVE 63
           STLTALA+E TLQ SFIRDEDERPK+ YN FS+E+PIISL GIDD   G+RAEIC KIV+
Sbjct: 7   STLTALAEEKTLQTSFIRDEDERPKVAYNQFSNEIPIISLEGIDDET-GKRAEICDKIVK 66

Query: 64  ACEDWGIFQVVDHGVDSELISEMTRLAREFFALPPEEKLRFDMSGGKKGGFIVSSHLKGE 123
           ACEDWG+FQVVDHGVD+E+IS+MT  A+EFFALPPEEKLRFDMSGGKKGGFIVSSHL+GE
Sbjct: 67  ACEDWGVFQVVDHGVDAEVISQMTTFAKEFFALPPEEKLRFDMSGGKKGGFIVSSHLQGE 126

Query: 124 AVKDWREIVTYFAYPIRNRNYSLWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAMGLE 183
            V+DWREIVTYF+YP R R+YS WPDKPEGW A T +YSEKLM LACKLL+VLSEAMGLE
Sbjct: 127 VVQDWREIVTYFSYPTRARDYSRWPDKPEGWIAVTQKYSEKLMELACKLLDVLSEAMGLE 186

Query: 184 KEALTKACVDMDQKIVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDGGR 243
           KEALTKACVDMDQK+VVNFYPKCP+PDLTLGLKRHTDPGTITLLLQDQVGGLQATKD G+
Sbjct: 187 KEALTKACVDMDQKVVVNFYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDNGK 246

Query: 244 SWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNTSRLSIATFQNPAPEAIVYP 303
           +WITVQPVEGAFVVNLGDHGH+LSNGRFKNADHQAVVNSN+SRLSIATFQNPAPEAIVYP
Sbjct: 247 TWITVQPVEGAFVVNLGDHGHFLSNGRFKNADHQAVVNSNSSRLSIATFQNPAPEAIVYP 306

Query: 304 LKIRDGEKPILEKPITFTEMYQSKMSRDLELARLKKLAHQQ 345
           LKIR+GEK I+++PITF EMY+ KMS+DLELARLKK A +Q
Sbjct: 307 LKIREGEKSIMDEPITFAEMYRRKMSKDLELARLKKQAKEQ 346

BLAST of Tan0008195 vs. ExPASy Swiss-Prot
Match: Q9S818 (Naringenin,2-oxoglutarate 3-dioxygenase OS=Arabidopsis thaliana OX=3702 GN=F3H PE=1 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 2.3e-169
Identity = 286/361 (79.22%), Postives = 323/361 (89.47%), Query Frame = 0

Query: 1   MASSTLTALADEPTLQASFIRDEDERPKIPYNHFSDEVPIISLNGIDDTAGGRRAEICRK 60
           MA  TLT LA E  L + F+RDEDERPK+ YN FSDE+P+ISL GIDD   G+R EICR+
Sbjct: 1   MAPGTLTELAGESKLNSKFVRDEDERPKVAYNVFSDEIPVISLAGIDD-VDGKRGEICRQ 60

Query: 61  IVEACEDWGIFQVVDHGVDSELISEMTRLAREFFALPPEEKLRFDMSGGKKGGFIVSSHL 120
           IVEACE+WGIFQVVDHGVD+ L+++MTRLAR+FFALPPE+KLRFDMSGGKKGGFIVSSHL
Sbjct: 61  IVEACENWGIFQVVDHGVDTNLVADMTRLARDFFALPPEDKLRFDMSGGKKGGFIVSSHL 120

Query: 121 KGEAVKDWREIVTYFAYPIRNRNYSLWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAM 180
           +GEAV+DWREIVTYF+YP+RNR+YS WPDKPEGW   T EYSE+LM LACKLLEVLSEAM
Sbjct: 121 QGEAVQDWREIVTYFSYPVRNRDYSRWPDKPEGWVKVTEEYSERLMSLACKLLEVLSEAM 180

Query: 181 GLEKEALTKACVDMDQKIVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 240
           GLEKE+LT ACVDMDQKIVVN+YPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQAT+D
Sbjct: 181 GLEKESLTNACVDMDQKIVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRD 240

Query: 241 GGRSWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNTSRLSIATFQNPAPEAI 300
            G++WITVQPVEGAFVVNLGDHGH+LSNGRFKNADHQAVVNSN+SRLSIATFQNPAP+A 
Sbjct: 241 NGKTWITVQPVEGAFVVNLGDHGHFLSNGRFKNADHQAVVNSNSSRLSIATFQNPAPDAT 300

Query: 301 VYPLKIRDGEKPILEKPITFTEMYQSKMSRDLELARLKKLAHQQHSSELLDQAQPNGQIF 360
           VYPLK+R+GEK ILE+PITF EMY+ KM RDLELARLKKLA ++   + +D  +P  QIF
Sbjct: 301 VYPLKVREGEKAILEEPITFAEMYKRKMGRDLELARLKKLAKEERDHKEVD--KPVDQIF 358

Query: 361 A 362
           A
Sbjct: 361 A 358

BLAST of Tan0008195 vs. ExPASy Swiss-Prot
Match: Q7XZQ7 (Flavanone 3-dioxygenase OS=Petroselinum crispum OX=4043 GN=FHT PE=1 SV=1)

HSP 1 Score: 590.5 bits (1521), Expect = 1.3e-167
Identity = 278/344 (80.81%), Postives = 316/344 (91.86%), Query Frame = 0

Query: 1   MASSTLTALADEPTLQASFIRDEDERPKIPYNHFSDEVPIISLNGIDDTAGGRRAEICRK 60
           MA STLTALA E TL + F+RDEDERPKI YN FSDE+P+ISL GIDD +  +R++ICRK
Sbjct: 1   MAPSTLTALAQEKTLNSKFVRDEDERPKIAYNKFSDEIPVISLAGIDDDSVDKRSQICRK 60

Query: 61  IVEACEDWGIFQVVDHGVDSELISEMTRLAREFFALPPEEKLRFDMSGGKKGGFIVSSHL 120
           IVEACEDWGIFQVVDHG+D +LISEMTRLAR+FFALP EEKLRFDM+GGKKGGFIVSSHL
Sbjct: 61  IVEACEDWGIFQVVDHGIDIDLISEMTRLARQFFALPAEEKLRFDMTGGKKGGFIVSSHL 120

Query: 121 KGEAVKDWREIVTYFAYPIRNRNYSLWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAM 180
           +GEAV+DWREIVTYF+YPI+ R+YS WPDKPEGW + T  YS++LM LACKLLEVLSEAM
Sbjct: 121 QGEAVQDWREIVTYFSYPIQARDYSRWPDKPEGWRSITEMYSDELMALACKLLEVLSEAM 180

Query: 181 GLEKEALTKACVDMDQKIVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 240
           GLEKE LTKACVDMDQK++VN+YPKCPQP+LTLGLKRHTDPGTITLLLQDQVGGLQAT+D
Sbjct: 181 GLEKEGLTKACVDMDQKVIVNYYPKCPQPNLTLGLKRHTDPGTITLLLQDQVGGLQATRD 240

Query: 241 GGRSWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNTSRLSIATFQNPAPEAI 300
           GG++WITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQAVVNSN+SR+SIATFQNPAP A 
Sbjct: 241 GGKTWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNSSRMSIATFQNPAPNAT 300

Query: 301 VYPLKIRDGEKPILEKPITFTEMYQSKMSRDLELARLKKLAHQQ 345
           VYPLKIR+GEK ++E+PITF EMY+ KMSRD+E+A LKKLA ++
Sbjct: 301 VYPLKIREGEKAVMEEPITFAEMYKRKMSRDIEMATLKKLAKEK 344

BLAST of Tan0008195 vs. ExPASy Swiss-Prot
Match: Q06942 (Naringenin,2-oxoglutarate 3-dioxygenase OS=Malus domestica OX=3750 PE=2 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 2.2e-167
Identity = 283/347 (81.56%), Postives = 316/347 (91.07%), Query Frame = 0

Query: 3   SSTLTALADEPTLQASFIRDEDERPKIPYNHFSDEVPIISLNGIDDTAGGRRAEICRKIV 62
           ++TLT++A E TLQ  F+RDEDERPK+ YN FS+E+PIISL GID+   GRR EIC+KIV
Sbjct: 4   ATTLTSIAHEKTLQQKFVRDEDERPKVAYNDFSNEIPIISLAGIDEVE-GRRGEICKKIV 63

Query: 63  EACEDWGIFQVVDHGVDSELISEMTRLAREFFALPPEEKLRFDMSGGKKGGFIVSSHLKG 122
            ACEDWGIFQ+VDHGVD+ELISEMT LAREFFALP EEKLRFDMSGGKKGGFIVSSHL+G
Sbjct: 64  AACEDWGIFQIVDHGVDAELISEMTGLAREFFALPSEEKLRFDMSGGKKGGFIVSSHLQG 123

Query: 123 EAVKDWREIVTYFAYPIRNRNYSLWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAMGL 182
           EAV+DWREIVTYF+YPIR+R+YS WPDKPE W   T +YS++LMGLACKLL VLSEAMGL
Sbjct: 124 EAVQDWREIVTYFSYPIRHRDYSRWPDKPEAWREVTKKYSDELMGLACKLLGVLSEAMGL 183

Query: 183 EKEALTKACVDMDQKIVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDGG 242
           + EALTKACVDMDQK+VVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQAT+D G
Sbjct: 184 DTEALTKACVDMDQKVVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRDDG 243

Query: 243 RSWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNTSRLSIATFQNPAPEAIVY 302
           ++WITVQPVEGAFVVNLGDHGH LSNGRFKNADHQAVVNSN+SRLSIATFQNPA EAIVY
Sbjct: 244 KTWITVQPVEGAFVVNLGDHGHLLSNGRFKNADHQAVVNSNSSRLSIATFQNPAQEAIVY 303

Query: 303 PLKIRDGEKPILEKPITFTEMYQSKMSRDLELARLKKLAHQQHSSEL 350
           PL +R+GEKPILE PIT+TEMY+ KMS+DLELARLKKLA +Q S +L
Sbjct: 304 PLSVREGEKPILEAPITYTEMYKKKMSKDLELARLKKLAKEQQSQDL 349

BLAST of Tan0008195 vs. NCBI nr
Match: XP_022939196.1 (naringenin,2-oxoglutarate 3-dioxygenase [Cucurbita moschata])

HSP 1 Score: 660.6 bits (1703), Expect = 7.7e-186
Identity = 325/369 (88.08%), Postives = 342/369 (92.68%), Query Frame = 0

Query: 1   MASSTLTALADEPTLQASFIRDEDERPKIPYNHFSDEVPIISLNGIDDTAGGRRAEICRK 60
           MASSTLTALAD+P LQ SFIRDEDERPKIPYNHFSDEVPIISL GIDD AGGRRAEICRK
Sbjct: 1   MASSTLTALADQPALQPSFIRDEDERPKIPYNHFSDEVPIISLAGIDDVAGGRRAEICRK 60

Query: 61  IVEACEDWGIFQVVDHGVDSELISEMTRLAREFFALPPEEKLRFDMSGGKKGGFIVSSHL 120
           IVEACEDWGIFQVVDHGVDS+LI+EMTRLAREFFALP EEKLRFDMSGGKKGGFIVSSHL
Sbjct: 61  IVEACEDWGIFQVVDHGVDSDLITEMTRLAREFFALPAEEKLRFDMSGGKKGGFIVSSHL 120

Query: 121 KGEAVKDWREIVTYFAYPIRNRNYSLWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAM 180
           KGE VKDWREIVTYF+YPIRNR+Y+ WPDKPEGWAA+TAEYSEKLMGLACKLLEVLSEAM
Sbjct: 121 KGEVVKDWREIVTYFSYPIRNRDYTRWPDKPEGWAARTAEYSEKLMGLACKLLEVLSEAM 180

Query: 181 GLEKEALTKACVDMDQKIVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 240
           GLEKEALTKACVDMDQKIVVNFYPKC QPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD
Sbjct: 181 GLEKEALTKACVDMDQKIVVNFYPKCLQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 240

Query: 241 GGRSWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNTSRLSIATFQNPAPEAI 300
           GG+SWITV+PV GAFVVNLGDHGHYLSNGRFKNADHQAVVNSNTSRLSIATFQNPAPEAI
Sbjct: 241 GGKSWITVRPVPGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNTSRLSIATFQNPAPEAI 300

Query: 301 VYPLKIRDGEKPILEKPITFTEMYQSKMSRDLELARLKKLAHQQHSSELLDQAQ------ 360
           VYPLK+R+GEKPILE PITF++MYQ+KMSRDLELARLKK A QQ + +   Q Q      
Sbjct: 301 VYPLKVREGEKPILENPITFSQMYQTKMSRDLELARLKKQAQQQQAQQQQAQQQAHFEPK 360

Query: 361 --PNGQIFA 362
             PN +IFA
Sbjct: 361 PKPNDKIFA 369

BLAST of Tan0008195 vs. NCBI nr
Match: KAG6596669.1 (Naringenin,2-oxoglutarate 3-dioxygenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 658.7 bits (1698), Expect = 2.9e-185
Identity = 322/363 (88.71%), Postives = 342/363 (94.21%), Query Frame = 0

Query: 1   MASSTLTALADEPTLQASFIRDEDERPKIPYNHFSDEVPIISLNGIDDTAGGRRAEICRK 60
           MASSTLTALAD+P LQ SFIRDEDERPKIPYNHFSDEVPIISL GIDD AGGRRAEICRK
Sbjct: 1   MASSTLTALADQPALQPSFIRDEDERPKIPYNHFSDEVPIISLAGIDDVAGGRRAEICRK 60

Query: 61  IVEACEDWGIFQVVDHGVDSELISEMTRLAREFFALPPEEKLRFDMSGGKKGGFIVSSHL 120
           IVEACEDWGIFQVVDHGVDS+LI+EMTRLAREFFALP EEKLRFDMSGGKKGGFIVSSHL
Sbjct: 61  IVEACEDWGIFQVVDHGVDSDLITEMTRLAREFFALPAEEKLRFDMSGGKKGGFIVSSHL 120

Query: 121 KGEAVKDWREIVTYFAYPIRNRNYSLWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAM 180
           KGE VKDWREI+TYF+YPIRNR+Y+ WPDKPEGWAA+TAEYSEKLMGLACKLLEVLSEAM
Sbjct: 121 KGEVVKDWREIMTYFSYPIRNRDYTRWPDKPEGWAARTAEYSEKLMGLACKLLEVLSEAM 180

Query: 181 GLEKEALTKACVDMDQKIVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 240
           GLEKEALTKACVDMDQKIVVNFYPKC QPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD
Sbjct: 181 GLEKEALTKACVDMDQKIVVNFYPKCLQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 240

Query: 241 GGRSWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNTSRLSIATFQNPAPEAI 300
           GG+SWITV+PV GAFVVNLGDHGHYLSNGRFKNADHQAVVNSNTSRLSIATFQNPAPEAI
Sbjct: 241 GGKSWITVRPVPGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNTSRLSIATFQNPAPEAI 300

Query: 301 VYPLKIRDGEKPILEKPITFTEMYQSKMSRDLELARLKKLAHQQ--HSSELLDQAQPNGQ 360
           VYPLK+R+GEKPILE PITF++MYQ+KMSRDLELARLKK A QQ   + +   + +PN +
Sbjct: 301 VYPLKVREGEKPILENPITFSQMYQTKMSRDLELARLKKQAQQQQAQTQQAHFEPKPNDK 360

Query: 361 IFA 362
           IFA
Sbjct: 361 IFA 363

BLAST of Tan0008195 vs. NCBI nr
Match: XP_023539914.1 (naringenin,2-oxoglutarate 3-dioxygenase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 657.5 bits (1695), Expect = 6.5e-185
Identity = 321/361 (88.92%), Postives = 339/361 (93.91%), Query Frame = 0

Query: 1   MASSTLTALADEPTLQASFIRDEDERPKIPYNHFSDEVPIISLNGIDDTAGGRRAEICRK 60
           MASSTLT+LAD+P LQ SFIRDEDERPKIPYNHFSDEVPIISL GIDD  GGRRAEICRK
Sbjct: 1   MASSTLTSLADQPALQPSFIRDEDERPKIPYNHFSDEVPIISLAGIDDVTGGRRAEICRK 60

Query: 61  IVEACEDWGIFQVVDHGVDSELISEMTRLAREFFALPPEEKLRFDMSGGKKGGFIVSSHL 120
           IVEACEDWGIFQVVDHGVDS+LISEMTRLAREFFALP EEKLRFDMSGGKKGGFIVSSHL
Sbjct: 61  IVEACEDWGIFQVVDHGVDSDLISEMTRLAREFFALPAEEKLRFDMSGGKKGGFIVSSHL 120

Query: 121 KGEAVKDWREIVTYFAYPIRNRNYSLWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAM 180
           KGE VKDWREIVTYF+YPIRNR+Y+ WPDKPEGWA +TAEYSEKLMGLACKLLEVLSEAM
Sbjct: 121 KGEVVKDWREIVTYFSYPIRNRDYTRWPDKPEGWATRTAEYSEKLMGLACKLLEVLSEAM 180

Query: 181 GLEKEALTKACVDMDQKIVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 240
           GLEKEALTKACVDMDQKIVVNFYPKC QPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD
Sbjct: 181 GLEKEALTKACVDMDQKIVVNFYPKCVQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 240

Query: 241 GGRSWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNTSRLSIATFQNPAPEAI 300
           GG+SWITV+PV GAFVVNLGDHGHYLSNGRFKNADHQAVVNSNTSRLSIATFQNPAPEAI
Sbjct: 241 GGKSWITVRPVPGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNTSRLSIATFQNPAPEAI 300

Query: 301 VYPLKIRDGEKPILEKPITFTEMYQSKMSRDLELARLKKLAHQQHSSELLDQAQPNGQIF 360
           VYPLK+R+GEKPILE PITF++MYQ+KMSRDLELARLKK A QQ  +    + +PN +IF
Sbjct: 301 VYPLKVREGEKPILENPITFSQMYQTKMSRDLELARLKKQA-QQQQAHFEPKPKPNDKIF 360

Query: 361 A 362
           A
Sbjct: 361 A 360

BLAST of Tan0008195 vs. NCBI nr
Match: KAG7028205.1 (Naringenin,2-oxoglutarate 3-dioxygenase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 656.4 bits (1692), Expect = 1.5e-184
Identity = 318/348 (91.38%), Postives = 334/348 (95.98%), Query Frame = 0

Query: 1   MASSTLTALADEPTLQASFIRDEDERPKIPYNHFSDEVPIISLNGIDDTAGGRRAEICRK 60
           MASSTLTALAD+P LQ SFIRDEDERPKIPYNHFSDEVPIISL GIDD AGGRRAEICRK
Sbjct: 1   MASSTLTALADQPALQPSFIRDEDERPKIPYNHFSDEVPIISLAGIDDVAGGRRAEICRK 60

Query: 61  IVEACEDWGIFQVVDHGVDSELISEMTRLAREFFALPPEEKLRFDMSGGKKGGFIVSSHL 120
           IVEACEDWGIFQVVDHGVDS+LI+EMTRLAREFFALP EEKLRFDMSGGKKGGFIVSSHL
Sbjct: 61  IVEACEDWGIFQVVDHGVDSDLITEMTRLAREFFALPAEEKLRFDMSGGKKGGFIVSSHL 120

Query: 121 KGEAVKDWREIVTYFAYPIRNRNYSLWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAM 180
           KGE VKDWREIVTYF+YPIRNR+Y+ WPDKPEGWAA+TAEYSEKLMGLACKLLEVLSEAM
Sbjct: 121 KGEVVKDWREIVTYFSYPIRNRDYTRWPDKPEGWAARTAEYSEKLMGLACKLLEVLSEAM 180

Query: 181 GLEKEALTKACVDMDQKIVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 240
           GLEKEALTKACVDMDQKIVVNFYPKC QPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD
Sbjct: 181 GLEKEALTKACVDMDQKIVVNFYPKCLQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 240

Query: 241 GGRSWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNTSRLSIATFQNPAPEAI 300
           GG+SWITV+PV GAFVVNLGDHGHYLSNGRFKNADHQAVVNSNTSRLSIATFQNPAPEAI
Sbjct: 241 GGKSWITVRPVPGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNTSRLSIATFQNPAPEAI 300

Query: 301 VYPLKIRDGEKPILEKPITFTEMYQSKMSRDLELARLKKLAHQQHSSE 349
           VYPLK+R+GEKPILE PITF++MYQ+KMSRDLELARLKK A QQ + +
Sbjct: 301 VYPLKVREGEKPILENPITFSQMYQTKMSRDLELARLKKQAQQQQAQQ 348

BLAST of Tan0008195 vs. NCBI nr
Match: XP_023005621.1 (naringenin,2-oxoglutarate 3-dioxygenase-like [Cucurbita maxima])

HSP 1 Score: 655.2 bits (1689), Expect = 3.2e-184
Identity = 322/364 (88.46%), Postives = 338/364 (92.86%), Query Frame = 0

Query: 1   MASSTLTALADEPTLQASFIRDEDERPKIPYNHFSDEVPIISLNGIDDTAGGRRAEICRK 60
           MASSTLTAL+D+P LQ SFIRDEDERPKIPYNHFSDEVPIISL GIDD AGGRRAEICRK
Sbjct: 1   MASSTLTALSDQPALQPSFIRDEDERPKIPYNHFSDEVPIISLAGIDDVAGGRRAEICRK 60

Query: 61  IVEACEDWGIFQVVDHGVDSELISEMTRLAREFFALPPEEKLRFDMSGGKKGGFIVSSHL 120
           IVEACEDWGIFQVVDHGVD +LISEMTRLAREFFALP EEKLRFDMSGGKKGGFIVSSHL
Sbjct: 61  IVEACEDWGIFQVVDHGVDLDLISEMTRLAREFFALPAEEKLRFDMSGGKKGGFIVSSHL 120

Query: 121 KGEAVKDWREIVTYFAYPIRNRNYSLWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAM 180
           KGE VKDWREIVTYF+YPIRNR+Y+ WPDKPEGWAA+T EYSEKLMGLACKLLEVLSEAM
Sbjct: 121 KGEVVKDWREIVTYFSYPIRNRDYTRWPDKPEGWAARTVEYSEKLMGLACKLLEVLSEAM 180

Query: 181 GLEKEALTKACVDMDQKIVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 240
           GLEKEALTKACVDMDQKIVVNFYPKC QPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD
Sbjct: 181 GLEKEALTKACVDMDQKIVVNFYPKCLQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 240

Query: 241 GGRSWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNTSRLSIATFQNPAPEAI 300
           GG+SWITV+PV GAFVVNLGDHGHYLSNGRFKNADHQAVVNSNTSRLSIATFQNPAPEAI
Sbjct: 241 GGKSWITVRPVPGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNTSRLSIATFQNPAPEAI 300

Query: 301 VYPLKIRDGEKPILEKPITFTEMYQSKMSRDLELARLKKLAHQQH---SSELLDQAQPNG 360
           VYPLK+R+GEKPILE PITF+EMYQ KMSRDLELARLKK A QQ      +   + +PN 
Sbjct: 301 VYPLKVREGEKPILENPITFSEMYQIKMSRDLELARLKKQAQQQQQQAQQQAHFEPKPND 360

Query: 361 QIFA 362
           +IFA
Sbjct: 361 KIFA 364

BLAST of Tan0008195 vs. ExPASy TrEMBL
Match: A0A6J1FF87 (naringenin,2-oxoglutarate 3-dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111445185 PE=3 SV=1)

HSP 1 Score: 660.6 bits (1703), Expect = 3.7e-186
Identity = 325/369 (88.08%), Postives = 342/369 (92.68%), Query Frame = 0

Query: 1   MASSTLTALADEPTLQASFIRDEDERPKIPYNHFSDEVPIISLNGIDDTAGGRRAEICRK 60
           MASSTLTALAD+P LQ SFIRDEDERPKIPYNHFSDEVPIISL GIDD AGGRRAEICRK
Sbjct: 1   MASSTLTALADQPALQPSFIRDEDERPKIPYNHFSDEVPIISLAGIDDVAGGRRAEICRK 60

Query: 61  IVEACEDWGIFQVVDHGVDSELISEMTRLAREFFALPPEEKLRFDMSGGKKGGFIVSSHL 120
           IVEACEDWGIFQVVDHGVDS+LI+EMTRLAREFFALP EEKLRFDMSGGKKGGFIVSSHL
Sbjct: 61  IVEACEDWGIFQVVDHGVDSDLITEMTRLAREFFALPAEEKLRFDMSGGKKGGFIVSSHL 120

Query: 121 KGEAVKDWREIVTYFAYPIRNRNYSLWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAM 180
           KGE VKDWREIVTYF+YPIRNR+Y+ WPDKPEGWAA+TAEYSEKLMGLACKLLEVLSEAM
Sbjct: 121 KGEVVKDWREIVTYFSYPIRNRDYTRWPDKPEGWAARTAEYSEKLMGLACKLLEVLSEAM 180

Query: 181 GLEKEALTKACVDMDQKIVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 240
           GLEKEALTKACVDMDQKIVVNFYPKC QPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD
Sbjct: 181 GLEKEALTKACVDMDQKIVVNFYPKCLQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 240

Query: 241 GGRSWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNTSRLSIATFQNPAPEAI 300
           GG+SWITV+PV GAFVVNLGDHGHYLSNGRFKNADHQAVVNSNTSRLSIATFQNPAPEAI
Sbjct: 241 GGKSWITVRPVPGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNTSRLSIATFQNPAPEAI 300

Query: 301 VYPLKIRDGEKPILEKPITFTEMYQSKMSRDLELARLKKLAHQQHSSELLDQAQ------ 360
           VYPLK+R+GEKPILE PITF++MYQ+KMSRDLELARLKK A QQ + +   Q Q      
Sbjct: 301 VYPLKVREGEKPILENPITFSQMYQTKMSRDLELARLKKQAQQQQAQQQQAQQQAHFEPK 360

Query: 361 --PNGQIFA 362
             PN +IFA
Sbjct: 361 PKPNDKIFA 369

BLAST of Tan0008195 vs. ExPASy TrEMBL
Match: A0A6J1KVH7 (naringenin,2-oxoglutarate 3-dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC111498563 PE=3 SV=1)

HSP 1 Score: 655.2 bits (1689), Expect = 1.6e-184
Identity = 322/364 (88.46%), Postives = 338/364 (92.86%), Query Frame = 0

Query: 1   MASSTLTALADEPTLQASFIRDEDERPKIPYNHFSDEVPIISLNGIDDTAGGRRAEICRK 60
           MASSTLTAL+D+P LQ SFIRDEDERPKIPYNHFSDEVPIISL GIDD AGGRRAEICRK
Sbjct: 1   MASSTLTALSDQPALQPSFIRDEDERPKIPYNHFSDEVPIISLAGIDDVAGGRRAEICRK 60

Query: 61  IVEACEDWGIFQVVDHGVDSELISEMTRLAREFFALPPEEKLRFDMSGGKKGGFIVSSHL 120
           IVEACEDWGIFQVVDHGVD +LISEMTRLAREFFALP EEKLRFDMSGGKKGGFIVSSHL
Sbjct: 61  IVEACEDWGIFQVVDHGVDLDLISEMTRLAREFFALPAEEKLRFDMSGGKKGGFIVSSHL 120

Query: 121 KGEAVKDWREIVTYFAYPIRNRNYSLWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAM 180
           KGE VKDWREIVTYF+YPIRNR+Y+ WPDKPEGWAA+T EYSEKLMGLACKLLEVLSEAM
Sbjct: 121 KGEVVKDWREIVTYFSYPIRNRDYTRWPDKPEGWAARTVEYSEKLMGLACKLLEVLSEAM 180

Query: 181 GLEKEALTKACVDMDQKIVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 240
           GLEKEALTKACVDMDQKIVVNFYPKC QPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD
Sbjct: 181 GLEKEALTKACVDMDQKIVVNFYPKCLQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 240

Query: 241 GGRSWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNTSRLSIATFQNPAPEAI 300
           GG+SWITV+PV GAFVVNLGDHGHYLSNGRFKNADHQAVVNSNTSRLSIATFQNPAPEAI
Sbjct: 241 GGKSWITVRPVPGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNTSRLSIATFQNPAPEAI 300

Query: 301 VYPLKIRDGEKPILEKPITFTEMYQSKMSRDLELARLKKLAHQQH---SSELLDQAQPNG 360
           VYPLK+R+GEKPILE PITF+EMYQ KMSRDLELARLKK A QQ      +   + +PN 
Sbjct: 301 VYPLKVREGEKPILENPITFSEMYQIKMSRDLELARLKKQAQQQQQQAQQQAHFEPKPND 360

Query: 361 QIFA 362
           +IFA
Sbjct: 361 KIFA 364

BLAST of Tan0008195 vs. ExPASy TrEMBL
Match: A0A6J1CU28 (naringenin,2-oxoglutarate 3-dioxygenase OS=Momordica charantia OX=3673 GN=LOC111014783 PE=3 SV=1)

HSP 1 Score: 634.8 bits (1636), Expect = 2.2e-178
Identity = 312/365 (85.48%), Postives = 334/365 (91.51%), Query Frame = 0

Query: 4   STLTALADEPTLQASFIRDEDERPKIPYNHFSDEVPIISLNGIDDTAGGRRAEICRKIVE 63
           STLTALADE TL+ +F+RDEDERPK+P++ FSDEVPIISL GIDD  GGRRAEICRKIVE
Sbjct: 80  STLTALADEKTLRPTFVRDEDERPKVPHDLFSDEVPIISLAGIDDAEGGRRAEICRKIVE 139

Query: 64  ACEDWGIFQVVDHGVDSELISEMTRLAREFFALPPEEKLRFDMSGGKKGGFIVSSHLKGE 123
           ACEDWGIFQVVDHGVD+ LISEM+RLA EFFALPPEEKLRFDMSGGKKGGFIVSSHLKGE
Sbjct: 140 ACEDWGIFQVVDHGVDAALISEMSRLATEFFALPPEEKLRFDMSGGKKGGFIVSSHLKGE 199

Query: 124 AVKDWREIVTYFAYPIRNRNYSLWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAMGLE 183
           AV+DWREIVTYF+YPIRNR+YS WPDKPEGW AKT EYSEKLMGLACKLLEVLSEAMGLE
Sbjct: 200 AVQDWREIVTYFSYPIRNRDYSRWPDKPEGWVAKTEEYSEKLMGLACKLLEVLSEAMGLE 259

Query: 184 KEALTKACVDMDQKIVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDGGR 243
           KEALT ACVDMDQKIVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD GR
Sbjct: 260 KEALTNACVDMDQKIVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDNGR 319

Query: 244 SWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNTSRLSIATFQNPAPEAIVYP 303
           +WITVQPV GAFVVNLGDHGHYLSNGRFKNADHQAVVNS +SRLSIATFQNPAPEAIVYP
Sbjct: 320 TWITVQPVPGAFVVNLGDHGHYLSNGRFKNADHQAVVNSKSSRLSIATFQNPAPEAIVYP 379

Query: 304 LKIRDGEKPILEKPITFTEMYQSKMSRDLELARLKKLAHQQ--HSSELLD-----QAQPN 362
           LK+R+GE PILE PITF+EMYQ KMSRD+ELARLKKLA Q+   + ELLD     + +P 
Sbjct: 380 LKVREGENPILESPITFSEMYQRKMSRDIELARLKKLAQQEQAQAQELLDKPHLVEPKPM 439

BLAST of Tan0008195 vs. ExPASy TrEMBL
Match: A0A6J1HQP9 (naringenin,2-oxoglutarate 3-dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC111465227 PE=3 SV=1)

HSP 1 Score: 625.5 bits (1612), Expect = 1.3e-175
Identity = 296/342 (86.55%), Postives = 324/342 (94.74%), Query Frame = 0

Query: 3   SSTLTALADEPTLQASFIRDEDERPKIPYNHFSDEVPIISLNGIDDTAGGRRAEICRKIV 62
           +STLT LADEPTLQ  FIRDEDER K+PYNHFS+EVPIIS+ GIDD  GG RAEICRKI+
Sbjct: 6   TSTLTDLADEPTLQPDFIRDEDERSKVPYNHFSNEVPIISIAGIDDVEGGGRAEICRKII 65

Query: 63  EACEDWGIFQVVDHGVDSELISEMTRLAREFFALPPEEKLRFDMSGGKKGGFIVSSHLKG 122
           +ACEDWGIFQ+VDHGVDSE++S+M RLAREFFALP EEKLR+DMSGGKKGGFIVSSHLKG
Sbjct: 66  DACEDWGIFQIVDHGVDSEIVSDMARLAREFFALPSEEKLRYDMSGGKKGGFIVSSHLKG 125

Query: 123 EAVKDWREIVTYFAYPIRNRNYSLWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAMGL 182
           E VKDWREIVTYF+YPIRNR+YSLWPDKPEGWAAKTAEYSEK+MGL  K+L VLSEAMGL
Sbjct: 126 EVVKDWREIVTYFSYPIRNRDYSLWPDKPEGWAAKTAEYSEKMMGLGRKMLVVLSEAMGL 185

Query: 183 EKEALTKACVDMDQKIVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDGG 242
           EKEA+ KACVDMDQKIVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDGG
Sbjct: 186 EKEAIVKACVDMDQKIVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDGG 245

Query: 243 RSWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNTSRLSIATFQNPAPEAIVY 302
           R+WITVQPV+GAF+VNLGDH HYLSNGRFKNA+H+AVVNSN+SRLSIATFQNPAPEAIVY
Sbjct: 246 RTWITVQPVQGAFIVNLGDHSHYLSNGRFKNAEHRAVVNSNSSRLSIATFQNPAPEAIVY 305

Query: 303 PLKIRDGEKPILEKPITFTEMYQSKMSRDLELARLKKLAHQQ 345
           PLKIR+GEKPILEKPITF+EMYQ+KM+RD+ELA+LKK A QQ
Sbjct: 306 PLKIREGEKPILEKPITFSEMYQTKMNRDIELAKLKKQAQQQ 347

BLAST of Tan0008195 vs. ExPASy TrEMBL
Match: A0A1Z1G625 (Flavanone 3-hydroxylase OS=Vernicia fordii OX=73154 PE=2 SV=1)

HSP 1 Score: 622.9 bits (1605), Expect = 8.6e-175
Identity = 300/364 (82.42%), Postives = 332/364 (91.21%), Query Frame = 0

Query: 1   MASSTLTALADEPTLQASFIRDEDERPKIPYNHFSDEVPIISLNGIDDTAGGRRAEICRK 60
           MA STLTALA+E TLQ SF+RDEDERPK+ YN FS+E+P+ISL GIDDT G +RAEIC+K
Sbjct: 1   MAPSTLTALAEEKTLQRSFVRDEDERPKVAYNQFSNEIPVISLAGIDDTEGNKRAEICKK 60

Query: 61  IVEACEDWGIFQVVDHGVDSELISEMTRLAREFFALPPEEKLRFDMSGGKKGGFIVSSHL 120
           IVEACEDWG+FQVVDHGVDS+LISEMTRLA+EFFALPPEEKLRFDMSGGKKGGFIVSSHL
Sbjct: 61  IVEACEDWGVFQVVDHGVDSKLISEMTRLAKEFFALPPEEKLRFDMSGGKKGGFIVSSHL 120

Query: 121 KGEAVKDWREIVTYFAYPIRNRNYSLWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAM 180
           +GEAV+DWREIVTYF+YP+R R+YS WPDKPEGW   T EYSEKLMGLACKLLEVLSEAM
Sbjct: 121 QGEAVQDWREIVTYFSYPLRTRDYSRWPDKPEGWIKVTEEYSEKLMGLACKLLEVLSEAM 180

Query: 181 GLEKEALTKACVDMDQKIVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 240
           GLEKEALTKACVDMDQK+VVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQAT+D
Sbjct: 181 GLEKEALTKACVDMDQKVVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRD 240

Query: 241 GGRSWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNTSRLSIATFQNPAPEAI 300
            G++WITVQPVEGAFVVNLGDHGH+LSNGRFKNADHQAVVNSN SRLSIATFQNPAPEA 
Sbjct: 241 NGKTWITVQPVEGAFVVNLGDHGHFLSNGRFKNADHQAVVNSNYSRLSIATFQNPAPEAT 300

Query: 301 VYPLKIRDGEKPILEKPITFTEMYQSKMSRDLELARLKKLAHQQHSSELLD---QAQPNG 360
           VYPLKI +G KP+LE+PITF EMY+ KMS+DLELARLKKLA +Q   +L     +++P  
Sbjct: 301 VYPLKIEEGAKPVLEEPITFAEMYRRKMSKDLELARLKKLAKEQQLKDLEKSKLESKPIE 360

Query: 361 QIFA 362
           +IFA
Sbjct: 361 EIFA 364

BLAST of Tan0008195 vs. TAIR 10
Match: AT3G51240.1 (flavanone 3-hydroxylase )

HSP 1 Score: 596.3 bits (1536), Expect = 1.7e-170
Identity = 286/361 (79.22%), Postives = 323/361 (89.47%), Query Frame = 0

Query: 1   MASSTLTALADEPTLQASFIRDEDERPKIPYNHFSDEVPIISLNGIDDTAGGRRAEICRK 60
           MA  TLT LA E  L + F+RDEDERPK+ YN FSDE+P+ISL GIDD   G+R EICR+
Sbjct: 1   MAPGTLTELAGESKLNSKFVRDEDERPKVAYNVFSDEIPVISLAGIDD-VDGKRGEICRQ 60

Query: 61  IVEACEDWGIFQVVDHGVDSELISEMTRLAREFFALPPEEKLRFDMSGGKKGGFIVSSHL 120
           IVEACE+WGIFQVVDHGVD+ L+++MTRLAR+FFALPPE+KLRFDMSGGKKGGFIVSSHL
Sbjct: 61  IVEACENWGIFQVVDHGVDTNLVADMTRLARDFFALPPEDKLRFDMSGGKKGGFIVSSHL 120

Query: 121 KGEAVKDWREIVTYFAYPIRNRNYSLWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAM 180
           +GEAV+DWREIVTYF+YP+RNR+YS WPDKPEGW   T EYSE+LM LACKLLEVLSEAM
Sbjct: 121 QGEAVQDWREIVTYFSYPVRNRDYSRWPDKPEGWVKVTEEYSERLMSLACKLLEVLSEAM 180

Query: 181 GLEKEALTKACVDMDQKIVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKD 240
           GLEKE+LT ACVDMDQKIVVN+YPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQAT+D
Sbjct: 181 GLEKESLTNACVDMDQKIVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRD 240

Query: 241 GGRSWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNTSRLSIATFQNPAPEAI 300
            G++WITVQPVEGAFVVNLGDHGH+LSNGRFKNADHQAVVNSN+SRLSIATFQNPAP+A 
Sbjct: 241 NGKTWITVQPVEGAFVVNLGDHGHFLSNGRFKNADHQAVVNSNSSRLSIATFQNPAPDAT 300

Query: 301 VYPLKIRDGEKPILEKPITFTEMYQSKMSRDLELARLKKLAHQQHSSELLDQAQPNGQIF 360
           VYPLK+R+GEK ILE+PITF EMY+ KM RDLELARLKKLA ++   + +D  +P  QIF
Sbjct: 301 VYPLKVREGEKAILEEPITFAEMYKRKMGRDLELARLKKLAKEERDHKEVD--KPVDQIF 358

Query: 361 A 362
           A
Sbjct: 361 A 358

BLAST of Tan0008195 vs. TAIR 10
Match: AT3G51240.2 (flavanone 3-hydroxylase )

HSP 1 Score: 477.6 bits (1228), Expect = 8.6e-135
Identity = 229/276 (82.97%), Postives = 254/276 (92.03%), Query Frame = 0

Query: 86  MTRLAREFFALPPEEKLRFDMSGGKKGGFIVSSHLKGEAVKDWREIVTYFAYPIRNRNYS 145
           MTRLAR+FFALPPE+KLRFDMSGGKKGGFIVSSHL+GEAV+DWREIVTYF+YP+RNR+YS
Sbjct: 1   MTRLARDFFALPPEDKLRFDMSGGKKGGFIVSSHLQGEAVQDWREIVTYFSYPVRNRDYS 60

Query: 146 LWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAMGLEKEALTKACVDMDQKIVVNFYPK 205
            WPDKPEGW   T EYSE+LM LACKLLEVLSEAMGLEKE+LT ACVDMDQKIVVN+YPK
Sbjct: 61  RWPDKPEGWVKVTEEYSERLMSLACKLLEVLSEAMGLEKESLTNACVDMDQKIVVNYYPK 120

Query: 206 CPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDGGRSWITVQPVEGAFVVNLGDHGHY 265
           CPQPDLTLGLKRHTDPGTITLLLQDQVGGLQAT+D G++WITVQPVEGAFVVNLGDHGH+
Sbjct: 121 CPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRDNGKTWITVQPVEGAFVVNLGDHGHF 180

Query: 266 LSNGRFKNADHQAVVNSNTSRLSIATFQNPAPEAIVYPLKIRDGEKPILEKPITFTEMYQ 325
           LSNGRFKNADHQAVVNSN+SRLSIATFQNPAP+A VYPLK+R+GEK ILE+PITF EMY+
Sbjct: 181 LSNGRFKNADHQAVVNSNSSRLSIATFQNPAPDATVYPLKVREGEKAILEEPITFAEMYK 240

Query: 326 SKMSRDLELARLKKLAHQQHSSELLDQAQPNGQIFA 362
            KM RDLELARLKKLA ++   + +D  +P  QIFA
Sbjct: 241 RKMGRDLELARLKKLAKEERDHKEVD--KPVDQIFA 274

BLAST of Tan0008195 vs. TAIR 10
Match: AT5G24530.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 218.4 bits (555), Expect = 9.4e-57
Identity = 121/339 (35.69%), Postives = 194/339 (57.23%), Query Frame = 0

Query: 1   MASSTLTALADEPTLQASFIRDEDERPKIPYNHFSDEVPIISLNGIDDTAGGRRAEICRK 60
           MA+  ++      TL  +++R   +RP++      ++ P+I L+  D      R+ + ++
Sbjct: 1   MAAKLISTGFRHTTLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTD------RSFLIQQ 60

Query: 61  IVEACEDWGIFQVVDHGVDSELISEMTRLAREFFALPPEEKLR-FDMSGGKKGGFIVSSH 120
           I +AC  +G FQV++HGV+ ++I EM  +AREFF++  EEK++ +     K      S +
Sbjct: 61  IHQACARFGFFQVINHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSFN 120

Query: 121 LKGEAVKDWREIVTYFAYPIRNRNYSLWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEA 180
           +K E V +WR+ +    YPI ++  + WP  P  +    ++YS ++  +  K+ E++SE+
Sbjct: 121 VKKEEVNNWRDYLRLHCYPI-HKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISES 180

Query: 181 MGLEKEALTKACVDMDQKIVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQD-QVGGLQAT 240
           +GLEK+ + K   +  Q + VN+YP CP+P+LT GL  HTDP  +T+LLQD  V GLQ  
Sbjct: 181 LGLEKDYMKKVLGEQGQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQIL 240

Query: 241 KDGGRSWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNTSRLSIATFQNPAPE 300
            DG   W  V P   AFV+N+GD    LSNG +K+  H+AV N+   RLS+A+F  PA  
Sbjct: 241 IDG--QWFAVNPHPDAFVINIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADC 300

Query: 301 AIVYPLK----IRDGEKPILEKPITFTEMYQSKMSRDLE 334
           A++ P K      D E   + K  T+ E Y+   SR+L+
Sbjct: 301 AVMSPAKPLWEAEDDETKPVYKDFTYAEYYKKFWSRNLD 330

BLAST of Tan0008195 vs. TAIR 10
Match: AT4G10490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 217.2 bits (552), Expect = 2.1e-56
Identity = 116/314 (36.94%), Postives = 178/314 (56.69%), Query Frame = 0

Query: 17  ASFIRDEDERPKIPYNHFS-DEVPIISLNGIDDTAGGRRAEICRKIVEACEDWGIFQVVD 76
           ++++R   +RPK+     S D +P+I L+   D  G  RA+I  +   AC   G FQ+ +
Sbjct: 20  SNYVRPVSDRPKMSEVQTSGDSIPLIDLH---DLHGPNRADIINQFAHACSSCGFFQIKN 79

Query: 77  HGVDSELISEMTRLAREFFALPPEEKLRFDMSGGKKGGFIVSS-HLKGEAVKDWREIVTY 136
           HGV  E I +M   AREFF     E+++   +  KK   + +S ++  E V +WR+ +  
Sbjct: 80  HGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSFNVSKEKVSNWRDFLRL 139

Query: 137 FAYPIRNRNYSLWPDKPEGWAAKTAEYSEKLMGLACKLLEVLSEAMGLEKEALTKACVDM 196
             YPI +   + WP  P  +   TAEY+  +  L   LLE +SE++GL K+ ++      
Sbjct: 140 HCYPIED-FINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIGKH 199

Query: 197 DQKIVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDGGRSWITVQPVEGA 256
            Q + +N+YP+CPQP+LT GL  H D   IT+LLQD+V GLQ  KDG   WI V PV   
Sbjct: 200 GQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDG--KWIAVNPVPNT 259

Query: 257 FVVNLGDHGHYLSNGRFKNADHQAVVNSNTSRLSIATFQNPAPEAIVYP---LKIRDGEK 316
           F+VNLGD    +SN ++K+  H+AVVNS+  R+SI TF  P+ +A++ P   L   + + 
Sbjct: 260 FIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDS 319

Query: 317 PILEKPITFTEMYQ 326
           P + +  T+ E ++
Sbjct: 320 PAIYRNFTYAEYFE 327

BLAST of Tan0008195 vs. TAIR 10
Match: AT4G10500.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 206.8 bits (525), Expect = 2.8e-53
Identity = 114/344 (33.14%), Postives = 184/344 (53.49%), Query Frame = 0

Query: 1   MASSTLTAL-----ADEPTLQASFIRDEDERPKIPYNHFS-DEVPIISLNGIDDTAGGRR 60
           MA+S ++ L     A    + ++++R   +RP +     S D +P+I L    D  G  R
Sbjct: 1   MATSAISKLLVSDFASSVHIPSNYVRPISDRPNLSEVESSGDSIPLIDLR---DLHGPNR 60

Query: 61  AEICRKIVEACEDWGIFQVVDHGVDSELISEMTRLAREFFALPPEEKLR-FDMSGGKKGG 120
           A I +++  AC  +G FQ+ +HGV    +++M  +AREFF  P  E+++ +     K   
Sbjct: 61  AVIVQQLASACSTYGFFQIKNHGVPDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTR 120

Query: 121 FIVSSHLKGEAVKDWREIVTYFAYPIRNRNYSLWPDKPEGWAAKTAEYSEKLMGLACKLL 180
              S ++  + V +WR+ +    +PI +     WP  P  +   TAEY+  +  L  +LL
Sbjct: 121 LSTSFNVGADKVLNWRDFLRLHCFPIED-FIEEWPSSPISFREVTAEYATSVRALVLRLL 180

Query: 181 EVLSEAMGLEKEALTKACVDMDQKIVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVG 240
           E +SE++GLE + ++       Q +  N+YP CP+P+LT GL  H DP  IT+LLQDQV 
Sbjct: 181 EAISESLGLESDHISNILGKHAQHMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQVS 240

Query: 241 GLQATKDGGRSWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNTSRLSIATFQ 300
           GLQ  KD    W+ V P+   F+VN+GD    +SN ++K+  H+AVVN+   RLSI TF 
Sbjct: 241 GLQVFKDD--KWVAVSPIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFY 300

Query: 301 NPAPEAIVYPLK--IRDGEKPILEKPITFTEMYQSKMSRDLELA 336
            P+ +A++ P    + + +   + +   F E +    +R L  A
Sbjct: 301 FPSTDAVIGPAHELVNEQDSLAIYRTYPFVEYWDKFWNRSLATA 338

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q059657.3e-17180.00Naringenin,2-oxoglutarate 3-dioxygenase (Fragment) OS=Matthiola incana OX=3724 G... [more]
Q073531.4e-16983.87Naringenin,2-oxoglutarate 3-dioxygenase (Fragment) OS=Petunia hybrida OX=4102 GN... [more]
Q9S8182.3e-16979.22Naringenin,2-oxoglutarate 3-dioxygenase OS=Arabidopsis thaliana OX=3702 GN=F3H P... [more]
Q7XZQ71.3e-16780.81Flavanone 3-dioxygenase OS=Petroselinum crispum OX=4043 GN=FHT PE=1 SV=1[more]
Q069422.2e-16781.56Naringenin,2-oxoglutarate 3-dioxygenase OS=Malus domestica OX=3750 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022939196.17.7e-18688.08naringenin,2-oxoglutarate 3-dioxygenase [Cucurbita moschata][more]
KAG6596669.12.9e-18588.71Naringenin,2-oxoglutarate 3-dioxygenase, partial [Cucurbita argyrosperma subsp. ... [more]
XP_023539914.16.5e-18588.92naringenin,2-oxoglutarate 3-dioxygenase [Cucurbita pepo subsp. pepo][more]
KAG7028205.11.5e-18491.38Naringenin,2-oxoglutarate 3-dioxygenase, partial [Cucurbita argyrosperma subsp. ... [more]
XP_023005621.13.2e-18488.46naringenin,2-oxoglutarate 3-dioxygenase-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1FF873.7e-18688.08naringenin,2-oxoglutarate 3-dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1KVH71.6e-18488.46naringenin,2-oxoglutarate 3-dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1CU282.2e-17885.48naringenin,2-oxoglutarate 3-dioxygenase OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A6J1HQP91.3e-17586.55naringenin,2-oxoglutarate 3-dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A1Z1G6258.6e-17582.42Flavanone 3-hydroxylase OS=Vernicia fordii OX=73154 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT3G51240.11.7e-17079.22flavanone 3-hydroxylase [more]
AT3G51240.28.6e-13582.97flavanone 3-hydroxylase [more]
AT5G24530.19.4e-5735.692-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G10490.12.1e-5636.942-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G10500.12.8e-5333.142-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 6..333
e-value: 5.6E-89
score: 300.7
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 196..294
e-value: 3.8E-23
score: 81.9
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 38..149
e-value: 2.8E-22
score: 79.7
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 8..345
NoneNo IPR availablePANTHERPTHR47990:SF11NARINGENIN,2-OXOGLUTARATE 3-DIOXYGENASEcoord: 8..345
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 9..330
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 191..295
score: 13.225734

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0008195.1Tan0008195.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding