Tan0007960 (gene) Snake gourd v1

Overview
NameTan0007960
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionE3 ubiquitin ligase BIG BROTHER-like
LocationLG07: 11806206 .. 11812519 (+)
RNA-Seq ExpressionTan0007960
SyntenyTan0007960
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCTGGAACTCAAACATCGAGGTTCATTGCATGAATAATGGTTATCCTTACAGCACGGCCTCTTTTATGGAATACTTTGAAGGTCTTACATATGATCATGTGAATTTCATTTTTTCCGGTCCCTCACATTCACAGGTACTATACGTCTCTTTGATTTTGGATGCCTTCCTTGGATCTCTTGTCTGTGAACTAGATTGATTGAACTCATGACATTTAGATGCTGTTTTGTCTGTACCTGCTATTCTGTAGCACTATATTGAAAAAAGTTATTTGACGTTACTTGTTGCCATGATGAGATCGTGCCTACATCTACTGTTTCTTTGCTTTGTTGGTTGTTTTTATGGATAATCTCTCTATCTCTTTCAAATTGCTCCCCTCGTCTTAAGATTTGTTGACAATTGACCCAAGAAGAAAAAAAAGGAAACCATGCCACATTTCATCTTCTAATTACTGATGCATCTTGCTTTAACTTCCTGATGCTTGATTGGACATTCATTCTCAAGATCAAATAGTCTTTATGTCTGATTTTCAAAATTCCAAATTGTTAAATCACCAAGCAACCCAAAAGCTTAAGCTAGTGGGTTACGGTAAATTTAATCTTATGTCAATACTCAAAATGCATAAAATTAATTTCCCAATATGGAAGTATAATGTTTTCATTCTTTCATTCAGGAGACTGTTTATCTGGGCATTCTGATTCTTGGAGCACTTCATGCTTTGATGCTCAATCGCTTGAAGTACTAGGTCATGAATCCACTATTGATGAACATAGGATGCCACAGGACTTCTCGACAATCCCGAATGAACAGAGTTTAGGAAATAGAGTGTGGGAGGAAAATGCCAACCCTAATATGGCCGGCAATAGCATGGAATGTAAGGATTGAGATCTAATTCGTTTTCGAATGAATACTCTAGTATATTTGTTTGTTAAATTGAAGTTTCCAAGTTAAGGTAATGGAACATCAATTCAGATATTGAACGTTAATATTCTATACAATCACATGCTGCAAAGGTTAATGACTCTCTATTTTGATTTTTTGTCTATTATTTTTTTCTCTGTTCATGAATTGAGGTACTTGTTCAGAAAAAGCAGTTGATGTCATCAATTTTCTGGTTAATTGGCAAACTTGATCTTTATGATGGCTATTGAGGGTATTATTTTTCCTGATTATAGGGCATTTTTGCATATTTGGAAATTCTAATGGTTAAATGGATCTGATGGTTCACTTGTGACTCAAATATGTAATAAAAATTACGTGTTGTCGTAAAATTGGCTTTAGGCATATTAGTCATTTTGTGAAATGGGTAACTCATTCAACGCAACAAATTGAATGTAGTGTGATTGAACAAGTATCTTATACTGATACACATATCTTCTTCCATATTTCAATGTTAACTGTAATTACTTGGGGAGCATCTTTATCTTTACCAAGTACTATTCTGATGTAGTATTAACTATGCAGCTAGCTTGAGTGTTAAAGTAAAATCTAATAACAAAATCATAATACAAGAAATATATATTTCTGTCATTTTTTAACATTCATTGTTGTTTGTCTCTTGGCTTCTTTCTTATTCAGTTGAATGTTTGTGGAGTTTTAAATACTAGTTCCCAATTCAACTATATTCCATTGCCTTTTCAGGCCCTCGGAGGCATTCAAATTATCAGGATTATCAGGTTTGCATCTTTTTGATTCATGAACTTTTTATGCCATTATGCTTATAGTTCTAGTTTTATTGTTAAGGAGGTTATAAGTGAATTACCTCACTTTTGCCTTAGTGTCCCTGCATTTTGTGAGTATGCATGCTTGCAATCAAGCACATGCTTATGTTGATACCTAAGATAAAATTATTCTTGCAAGACATAAAATTGGCTCATCTGAGACTACTGGAAAAAAGTAGAGGTAGAGAATATAGAATAGGAATTCATTTCTAACCCAATGTTGACAACCTAGGAGGTGGTTATGATTTTCTTAGGTCCCGTTTGATAACCATTTGGTTTTTTAAAATTGTGTCGGTTTTCTCACCATTTGTCTACAATGGTTTTCATTTTCTCTACCGTGGTTTCCATCTTTCTTAATGAACTATTTGAGTTTCTAGCGAAATTTAAAAAACAAAAATAAATTTTTGAAAACTACTTTTTTTAGTCTCAAAACTTAGTTTGATTTTTGAAAACATAGGTAAAAAATGGATAACAAAACATGGAAACCCATAGATGAAAGTAGTGTTTTTAGGCTTAATTTTCAAAAACTAAAAATTAAAAATCAAATGGTTATTCAACGGGGCCTTAATATCTTGGTTTTCAGATTTTTGTTCAAGGCCTAATAGACTTGGAATTTAGTTCCAACGCTCTTCAGAAGACTTCTAAATAAATGCATTCCCTAATCAGTTCCAGTTTGAATTATGCAGACTATTTGGCAAGATATTATTGATCCTGATAACATGACTTATGAGGTTAGTGCATCCGGTAGTTTTCTTTCCTCGTTACTTTTGCTTGTTACCGCACAAAATGTTTCCTCTCCTGTAAATTACATGACTAAGTTTCCTTCACTGCTTATTTATTACATGAATATTGCTAATTGAACTATTATTCCGGACTTGCTGCTGTATAAAGAAAATGGAAAAACTAGTAGCCTATTTATTATATGAATAGGCTTGGTTTTGGATGCTTTCTTGCTAGGGTAGAAAGGCAGGAAATGCTGGCTCAGATTAATTTAGTTGTTTATGGTACACTTCTAGCTGGTTCTCTAACCTTGAACTTGCTTCCTTGGAAGGCCTTATGTACTAAAAAGGAAAAAGAAAAGGCTTGCTTCTTTGGAGGGATCTTCCTGTTGATGTTGCTTTTTAAGAATCTAAAGTTTATTAGATCGGAGCGTAAGGAACCAGCTGTAAATCCACTTTTTACATTCTGAAATCAAGGGCTATGCTTAATAAACATAAAATTTGTCTGGCTAACTGTAATGTGTAAAGTCAAGTGTATGCCTCCTTAATTATGTCGATTACTCTCTTCAATAGAGTGACAGTTTAAAGCAACTTAATTTCAGGAATTAGTAGATCTAGGCGAGACAGTAGGAACTCAAAGTAGAGGCCTTTCGCAAGAACTGATTGCATTGCTTCCAGTATCGAAGTATAAATGTTGGTTTTTCTCAAGGAAGAAATCACGAAATGAAAGGTAACACTAGATTGATTGATTGTTTTATCCCGTATGATTTTTTGAACTTTTCCTATTTTTCTTTTGATTTTGAGGATTGTTCCAGCTACCGAGATGAATCAGAATTTCCGCAATGCTTGCAAGTCACTTTTGAAATGTTAAGGTTCACTAGAAATCTTTGGGCATGCTACAAAGTTCACTGTATTTATTTCTTAAAAGAATATTATCGTATTGTTTTACTATAGCGTTTCTCCATGTTTTTTTTTGTTATCTGTTGAATGAGCAATTATGTTGCTAGCCTGACTTAGATATCCTATTTAGCCATTAGCTAGCTAAAATTAAAAGCTGACTGAAATTGGTAATTATATGTGATCTTACTGAGTCAATTGACTTTTTGGTAAGATGTTAGATATTTATGCCAAACTAAGGACGTTATAAAAATTAAATTGCATGTACCATTGTCCTAGGAAGCTGTGGGCCGTTTACGTAGAACTATGGACCTATGGTGATATCCACTAACGGTCTAACGGAGAAGGCGGCTTCGATATTTAGTCGTACTTACCAATACCCTTGTATATATAGCTAAGTAATGATGTCCATGAATTCTAATATTTTCATGTTCATTATCAATATTAAGATTCATGTATACTTATGGCTTGGTCTTTCTCAAAACTATCTCATTAAAGAAAGAAAACACTTCAGAGTGGTGTGGTTGAGTAGCCTACGTCTGCTATTAATAACAGCACCATTTATTCAACAACGAAGAATACAATGATCATTAGACCCAAGAGATGTATAAGTCTTGCTCAACAACTCAGAGAATACTTTCAAAAAGTTTGAACCCTTTTCACTCAGAAAACTTTTGAGTCATAATCGATTACACTAAAAAACATACATTTTGTATATAGGTGTGTAATATGTCAAATGGAATACAAACGTGGAGATCAAAGGATCACTCTACCGTGCAAACACAGGTACCATACCAGTTGCGGGACCAAGTGGCTTAGTATCAACAAGGTAATGTTCTGATATCTTTTATCCTTGCTCTTTTGAGAATAATTTTAGGCCCAAACGATAACCATTTTATTTTTAGCTTTCTATTTTTGAAAATTGTGCCAGTTTTCTCCTGACTTCTTCTTTACAATGGTTTTCACATTTCTTGAAGAAATGTTTGAATAGTTAGCCAAATTCCAAAAACAAAAATGAGATTTTAAAAACTATGGCCCCGTTTGATTACCATTTGGTTTTTGGTTTCTAGTTTTTGAAAATTAAGCTTAAAAAATAAAAATGATACCCTCACCTATGAGTTTTCTTACTTTGTTATCTACTTTTTACCTAAGTTTTCAAAAATCAAGCCAAGTTTTAAAAACTAAAAAAAAAAAAATAGTTTTTGAAAACTTGATTTTGTTTTTTGAATTCGACTAAGAATTGAAATGTTTCCTTGAGAAAATGAAATTCAGGGTAGAGAAATTAGGAGAAAACAAACTCAATTTACAAAAACCAAAAGCAAAAAATGAAATGGTTATCAAATGAGGCCTATGTTTTTTAGTTTTCAAAATTTGGCTTGGATTTTGAAAACACTATTAGAAAGTAGATAACAAATCAAAGAAATTCATAGGTAGAAATACTAATTTTCAAAAACAAAAAACAAAAAACCAAGACCCTTTTTGATAACTATTTGATTTTTGAAATTGTGCTCGTTTTCTCACAATTTCTCTCCAATGGTTTTCATCTTCTCTACATAGGTTTCCATCTTTCTTAAGGAAATCTTTGAGTTTCTAGCCAAATTCCAAAAACAAAAACAAGTTTTTGAAAACTATTTTTTTAGTTTTCAAAACTTGGCTTGTTTGTGAAAACATAGGTAAAAAGTAGGATAAGAAAACATGAAAACTCATAGGTAGAAATACTATTTATGGGCTTAATTTTTAAAAATCAAAAAACAAAAAACAAAATGGTTATCAAACGGAGCTGAGGCCTCATTTGATAACCTTGATTTCTGGTTTTTGAAAATTAAGCATATAAATACTACTTTCACTTGGTTTTCTTGTTTTGTTATCTATTTTTTTACCTACATTTTTTTAATGAGCAAACCAAGCTTTCATAGAGAAAAAATGAAATAATACACAAGCGTACAAAAAAGAAAGCCCTCAAAAGGGAGCCCACTATAAAAGGGAGCCCACTATAAAAAAGGGCTCCAATCTAGTAAAATAAGACTAAGAGAATAATTACCAAAGTCCTCAACACTGAAACCCAAAGAGAAATGTAGAATCTAACAAAGGATCACACTCCCAAAGAAGAGGTCTTTCTCCCCTGAAAGATTCTATTATTTCTCTCCCTTCACAACCCCCACAAAATAACACACACCCCAGCTTGCCACAAAAATAAACTTTTCTCACGAAAGGGCAACCCAGTGAGAAGTTCTATAAACAACTCTCTGTTATTCAGATAACCCATGTAACTGAAATTGAACTACTGAAAGAAATAACTGCAGACTGCCCTCGCCAAATTACACAACCACAATAAATGATCAAGGTCTTCAGTTGTCTTCTTGCACAGAATACAACAGAAAGGACCTGACGAAGGGTACCCCCTGGCCAACACCCGATCTAAGGTGTTAACCCTTCCGTGCCAAATCTGCCAAACAAAAAACTTTACCTTCTTCGGGACCTTAATCTTCCAGAGAGAGGAGAAGGAGGAACCACCCGACCTATCCAAAAAAAACAAGACGCTAAAAAAAGGAGCTACAAGAAAAGCCTCTAGATGGGAAGGAGTCTAGAGGCGGGCATCCCGCCGATGAGGGCTGAAACAAAAATGGATGATTAAAGATAACAAAGAGACATTCCCTAGGTTAAAAAAAGACAACATCAACCGTTTCAATTTTTTACCTACATTTTTAAAAACTAAGTCAAGTTTTAAAGACTAAAAAAAGTGGTTTTCAAAAATTTATTTTTGTTTTTGGAATTTGACCAAGAATTCAAATGTTTTCTTGAGAAAATGAAACTTGAAAACAAGCAAAATTTTCAAACCAAAGCCCAAATGATATCAAAACAATGCCTTTGTAACATACTATCAGTAATTTTAGCCAATTCATGACATAATCTCTCTGTTCCAGGCTTGCCCCATTTGCTACACTGAGGTTTTTGGTGACAATTCAAACCGTTAA

mRNA sequence

ATGAGCTGGAACTCAAACATCGAGGTTCATTGCATGAATAATGGTTATCCTTACAGCACGGCCTCTTTTATGGAATACTTTGAAGGTCTTACATATGATCATGTGAATTTCATTTTTTCCGGTCCCTCACATTCACAGTGGGTTACGGTAAATTTAATCTTATGTCAATACTCAAAATGCATAAAATTAATTTCCCAATATGGAAGTATAATGTTTTCATTCTTTCATTCAGGAGACTGTTTATCTGGGCATTCTGATTCTTGGAGCACTTCATGCTTTGATGCTCAATCGCTTGAAGTACTAGGTCATGAATCCACTATTGATGAACATAGGATGCCACAGGACTTCTCGACAATCCCGAATGAACAGAGTTTAGGAAATAGAGTGTGGGAGGAAAATGCCAACCCTAATATGGCCGGCAATAGCATGGAATGCCCTCGGAGGCATTCAAATTATCAGGATTATCAGACTATTTGGCAAGATATTATTGATCCTGATAACATGACTTATGAGGAATTAGTAGATCTAGGCGAGACAGTAGGAACTCAAAGTAGAGGCCTTTCGCAAGAACTGATTGCATTGCTTCCAGTATCGAAGTATAAATGTTGGTTTTTCTCAAGGAAGAAATCACGAAATGAAAGGTGTGTAATATGTCAAATGGAATACAAACGTGGAGATCAAAGGATCACTCTACCGTGCAAACACAGGTACCATACCAGTTGCGGGACCAAGTGGCTTAGTATCAACAAGGCTTGCCCCATTTGCTACACTGAGGTTTTTGGTGACAATTCAAACCGTTAA

Coding sequence (CDS)

ATGAGCTGGAACTCAAACATCGAGGTTCATTGCATGAATAATGGTTATCCTTACAGCACGGCCTCTTTTATGGAATACTTTGAAGGTCTTACATATGATCATGTGAATTTCATTTTTTCCGGTCCCTCACATTCACAGTGGGTTACGGTAAATTTAATCTTATGTCAATACTCAAAATGCATAAAATTAATTTCCCAATATGGAAGTATAATGTTTTCATTCTTTCATTCAGGAGACTGTTTATCTGGGCATTCTGATTCTTGGAGCACTTCATGCTTTGATGCTCAATCGCTTGAAGTACTAGGTCATGAATCCACTATTGATGAACATAGGATGCCACAGGACTTCTCGACAATCCCGAATGAACAGAGTTTAGGAAATAGAGTGTGGGAGGAAAATGCCAACCCTAATATGGCCGGCAATAGCATGGAATGCCCTCGGAGGCATTCAAATTATCAGGATTATCAGACTATTTGGCAAGATATTATTGATCCTGATAACATGACTTATGAGGAATTAGTAGATCTAGGCGAGACAGTAGGAACTCAAAGTAGAGGCCTTTCGCAAGAACTGATTGCATTGCTTCCAGTATCGAAGTATAAATGTTGGTTTTTCTCAAGGAAGAAATCACGAAATGAAAGGTGTGTAATATGTCAAATGGAATACAAACGTGGAGATCAAAGGATCACTCTACCGTGCAAACACAGGTACCATACCAGTTGCGGGACCAAGTGGCTTAGTATCAACAAGGCTTGCCCCATTTGCTACACTGAGGTTTTTGGTGACAATTCAAACCGTTAA

Protein sequence

MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGPSHSQWVTVNLILCQYSKCIKLISQYGSIMFSFFHSGDCLSGHSDSWSTSCFDAQSLEVLGHESTIDEHRMPQDFSTIPNEQSLGNRVWEENANPNMAGNSMECPRRHSNYQDYQTIWQDIIDPDNMTYEELVDLGETVGTQSRGLSQELIALLPVSKYKCWFFSRKKSRNERCVICQMEYKRGDQRITLPCKHRYHTSCGTKWLSINKACPICYTEVFGDNSNR
Homology
BLAST of Tan0007960 vs. ExPASy Swiss-Prot
Match: Q8L649 (E3 ubiquitin-protein ligase BIG BROTHER OS=Arabidopsis thaliana OX=3702 GN=BB PE=1 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 9.2e-38
Identity = 97/258 (37.60%), Postives = 134/258 (51.94%), Query Frame = 0

Query: 8   EVHCMNNGYPY-STASFMEYFEGLTYDHVNFIFSGPSHSQWVTVNLILCQYSKCIKLISQ 67
           + H    G+PY +T S+M+++ G     +N+  +   H Q    NL     +   K    
Sbjct: 10  DAHYTETGFPYAATGSYMDFYGGAAQGPLNYDHAATMHPQ---DNLYWTMNTNAYK-FGF 69

Query: 68  YGSIMFSFFHSGDCLSGHSDSWSTSCFDAQSLEVLGHESTIDEHRMPQDFSTIPNEQSLG 127
            GS   SF+ S D ++ H    S               +  D H M        N  +  
Sbjct: 70  SGSDNASFYGSYD-MNDHLSRMSIG------------RTNWDYHPMVNVADDPENTVARS 129

Query: 128 NRVWEENANPNMAGNSMECPRRHSNYQDYQTIWQDIIDPDNMTYEELVDLGETVGTQSRG 187
            ++ + + +      + EC     +    Q  WQD IDPD MTYEELV+LGE VGT+SRG
Sbjct: 130 VQIGDTDEH----SEAEECIANEHDPDSPQVSWQDDIDPDTMTYEELVELGEAVGTESRG 189

Query: 188 LSQELIALLPVSKYKCWFFSRKKSRNERCVICQMEYKRGDQRITLPCKHRYHTSCGTKWL 247
           LSQELI  LP  KYK      +K   ERCVICQ++YK G++++ LPCKH YH+ C +KWL
Sbjct: 190 LSQELIETLPTKKYKFGSIFSRKRAGERCVICQLKYKIGERQMNLPCKHVYHSECISKWL 246

Query: 248 SINKACPICYTEVFGDNS 265
           SINK CP+C +EVFG+ S
Sbjct: 250 SINKVCPVCNSEVFGEPS 246

BLAST of Tan0007960 vs. ExPASy Swiss-Prot
Match: Q9LT17 (E3 ubiquitin ligase BIG BROTHER-related OS=Arabidopsis thaliana OX=3702 GN=BBR PE=2 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 2.6e-24
Identity = 53/111 (47.75%), Postives = 70/111 (63.06%), Query Frame = 0

Query: 156 QTIWQDIIDPDNMTYEELVDLGETVGTQSRGLSQELIALLPVSKYKCWFFSRKKSRNERC 215
           Q  W D +DPD ++YEEL+ LG+ VGT+SRGLS + IA LP  +YK      +   NE C
Sbjct: 232 QDAW-DEMDPDELSYEELLALGDIVGTESRGLSADTIASLPSKRYK--EGDNQNGTNESC 291

Query: 216 VICQMEYKRGDQRITLPCKHRYHTSCGTKWLSINKACPICYTEVFGDNSNR 267
           VIC+++Y+  +  I LPCKH YH+ C   WL INK CP+C  EV    S +
Sbjct: 292 VICRLDYEDDEDLILLPCKHSYHSECINNWLKINKVCPVCSAEVSTSTSGQ 339

BLAST of Tan0007960 vs. ExPASy Swiss-Prot
Match: O49500 (E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 1.3e-15
Identity = 45/97 (46.39%), Postives = 56/97 (57.73%), Query Frame = 0

Query: 163 IDPDNMTYEELVDLGETVGTQSRGLSQELIALLPVSKYKCWFFSRKKSRN--ERCVICQM 222
           +D DNM+YEEL+ LGE +G  S GLS+E+I  L V K      S   S    E C +CQ 
Sbjct: 567 LDVDNMSYEELLALGERIGDVSTGLSEEVI--LKVMKQHKHTSSAAGSHQDMEPCCVCQE 626

Query: 223 EYKRGDQRITLPCKHRYHTSCGTKWLSINKACPICYT 258
           EY  GD   TL C H +HT+C  +WL +   CPIC T
Sbjct: 627 EYAEGDDLGTLGCGHEFHTACVKQWLMLKNLCPICKT 661

BLAST of Tan0007960 vs. ExPASy Swiss-Prot
Match: Q9ZQF9 (E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 8.4e-15
Identity = 44/100 (44.00%), Postives = 56/100 (56.00%), Query Frame = 0

Query: 163 IDPDNMTYEELVDLGETVGTQSRGLSQELIALLPVSKYKCWFFSRKK----SRNERCVIC 222
           +D DNM+YEEL+ LGE +G  S GLS+E+I L  + ++K    S          E C IC
Sbjct: 601 LDVDNMSYEELLALGERIGDVSTGLSEEVI-LKAMKQHKHTSSSPSSVELHQNIEPCCIC 660

Query: 223 QMEYKRGDQRITLPCKHRYHTSCGTKWLSINKACPICYTE 259
           Q EY  GD   TL C H +H  C  +W+ I   CPIC TE
Sbjct: 661 QEEYVEGDNLGTLKCGHEFHKDCIKQWVMIKNLCPICKTE 699

BLAST of Tan0007960 vs. ExPASy Swiss-Prot
Match: Q7XTV7 (Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=39947 GN=HIP1 PE=1 SV=2)

HSP 1 Score: 81.3 bits (199), Expect = 1.9e-14
Identity = 41/94 (43.62%), Postives = 51/94 (54.26%), Query Frame = 0

Query: 163 IDPDNMTYEELVDLGETVGTQSRGLSQELIA-LLPVSKYKCWFFSRKKSRNERCVICQME 222
           +D DNM+YEEL+ L E +G  S GLS+E +  LL   K+  W         E C ICQ E
Sbjct: 568 LDIDNMSYEELLALEERIGNVSTGLSEEEVTKLLKQRKFSSWRL-EASVEEEPCCICQEE 627

Query: 223 YKRGDQRITLPCKHRYHTSCGTKWLSINKACPIC 256
           Y  GD   TL C H +H  C  +WL +   CPIC
Sbjct: 628 YVDGDDLGTLDCGHDFHVGCVRQWLVVKNTCPIC 660

BLAST of Tan0007960 vs. NCBI nr
Match: XP_038901510.1 (E3 ubiquitin-protein ligase BIG BROTHER-like [Benincasa hispida])

HSP 1 Score: 430.3 bits (1105), Expect = 1.3e-116
Identity = 210/266 (78.95%), Postives = 218/266 (81.95%), Query Frame = 0

Query: 1   MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGPSHSQWVTVNLILCQYSKC 60
           MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSG SHSQ     L    Y K 
Sbjct: 1   MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHSQETIYPLTNSSYYK- 60

Query: 61  IKLISQYGSIMFSFFHSGDCLSGHSDSWSTSCFDAQSLEVLGHESTIDEHRMPQDFSTIP 120
                                 GHSDSWSTS FDAQS E+ GHESTIDEHR  +  ST P
Sbjct: 61  ---------------------FGHSDSWSTSHFDAQSFEIQGHESTIDEHR--RLLSTAP 120

Query: 121 NEQSLGNRVWEENANPNMAGNSMECPRRHSNYQDYQTIWQDIIDPDNMTYEELVDLGETV 180
           NEQSLGNRVWEENANPNM+ N+MECPRRHSNY +YQTIWQDI+DPDNMTYEELVDLGETV
Sbjct: 121 NEQSLGNRVWEENANPNMSSNNMECPRRHSNYHEYQTIWQDIVDPDNMTYEELVDLGETV 180

Query: 181 GTQSRGLSQELIALLPVSKYKCWFFSRKKSRNERCVICQMEYKRGDQRITLPCKHRYHTS 240
           GTQSRGLSQELI+LLPVSKYKC FF R KSRNERCVICQMEYKRGDQRITLPCKHRYHTS
Sbjct: 181 GTQSRGLSQELISLLPVSKYKCGFFLRNKSRNERCVICQMEYKRGDQRITLPCKHRYHTS 240

Query: 241 CGTKWLSINKACPICYTEVFGDNSNR 267
           CGTKWLSINKACPICYTEVFGD+S R
Sbjct: 241 CGTKWLSINKACPICYTEVFGDSSKR 242

BLAST of Tan0007960 vs. NCBI nr
Match: XP_022995132.1 (E3 ubiquitin ligase BIG BROTHER-like [Cucurbita maxima] >XP_022995133.1 E3 ubiquitin ligase BIG BROTHER-like [Cucurbita maxima] >XP_022995134.1 E3 ubiquitin ligase BIG BROTHER-like [Cucurbita maxima] >XP_022995135.1 E3 ubiquitin ligase BIG BROTHER-like [Cucurbita maxima])

HSP 1 Score: 425.6 bits (1093), Expect = 3.1e-115
Identity = 203/266 (76.32%), Postives = 215/266 (80.83%), Query Frame = 0

Query: 1   MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGPSHSQWVTVNLILCQYSKC 60
           MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFS PSHS+          Y K 
Sbjct: 1   MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSAPSHSEETVCPATNSSYYK- 60

Query: 61  IKLISQYGSIMFSFFHSGDCLSGHSDSWSTSCFDAQSLEVLGHESTIDEHRMPQDFSTIP 120
                                  H+DSWS SCFD QS EV GHES IDEHRM Q+FST+P
Sbjct: 61  ---------------------FEHADSWSASCFDTQSFEVQGHESVIDEHRMQQEFSTVP 120

Query: 121 NEQSLGNRVWEENANPNMAGNSMECPRRHSNYQDYQTIWQDIIDPDNMTYEELVDLGETV 180
           +EQSLGNRV EEN NP+MA NSMECPRRHSNY +YQTIWQDIIDPDNMTYEELVDLGETV
Sbjct: 121 DEQSLGNRVREENVNPDMASNSMECPRRHSNYHEYQTIWQDIIDPDNMTYEELVDLGETV 180

Query: 181 GTQSRGLSQELIALLPVSKYKCWFFSRKKSRNERCVICQMEYKRGDQRITLPCKHRYHTS 240
           G+QSRGLSQELIALLPVSK+KCWFFSRKKSR ERCVICQM YK GDQRITLPCKH+YHTS
Sbjct: 181 GSQSRGLSQELIALLPVSKFKCWFFSRKKSRTERCVICQMNYKHGDQRITLPCKHKYHTS 240

Query: 241 CGTKWLSINKACPICYTEVFGDNSNR 267
           CG+KWLSINKACPICYTEV+GD S R
Sbjct: 241 CGSKWLSINKACPICYTEVYGDISKR 244

BLAST of Tan0007960 vs. NCBI nr
Match: XP_023532670.1 (E3 ubiquitin ligase BIG BROTHER-like [Cucurbita pepo subsp. pepo] >XP_023532671.1 E3 ubiquitin ligase BIG BROTHER-like [Cucurbita pepo subsp. pepo] >XP_023532672.1 E3 ubiquitin ligase BIG BROTHER-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 423.3 bits (1087), Expect = 1.5e-114
Identity = 203/266 (76.32%), Postives = 213/266 (80.08%), Query Frame = 0

Query: 1   MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGPSHSQWVTVNLILCQYSKC 60
           MSWNSNIEVHCM NGYPYSTASFMEYFEGLTYDHVNFIFSGPSHSQ          Y K 
Sbjct: 1   MSWNSNIEVHCMTNGYPYSTASFMEYFEGLTYDHVNFIFSGPSHSQETVCPATNSSYYK- 60

Query: 61  IKLISQYGSIMFSFFHSGDCLSGHSDSWSTSCFDAQSLEVLGHESTIDEHRMPQDFSTIP 120
                                  H+DSWS SCFD QS EV GHES IDEHRM Q+FST+P
Sbjct: 61  ---------------------FEHADSWSASCFDTQSFEVQGHESAIDEHRMQQEFSTVP 120

Query: 121 NEQSLGNRVWEENANPNMAGNSMECPRRHSNYQDYQTIWQDIIDPDNMTYEELVDLGETV 180
           +EQSLGNR  EEN NP+MA NSMECPRRHSNY +YQTIWQDIIDPDNMTYEELVDLGETV
Sbjct: 121 DEQSLGNRAREENVNPDMASNSMECPRRHSNYHEYQTIWQDIIDPDNMTYEELVDLGETV 180

Query: 181 GTQSRGLSQELIALLPVSKYKCWFFSRKKSRNERCVICQMEYKRGDQRITLPCKHRYHTS 240
           G+QSRGLSQELIALLPVSK+KCW FSRKKSR ERCVICQM YK GDQRITLPCKH+YHTS
Sbjct: 181 GSQSRGLSQELIALLPVSKFKCWLFSRKKSRTERCVICQMNYKYGDQRITLPCKHKYHTS 240

Query: 241 CGTKWLSINKACPICYTEVFGDNSNR 267
           CG+KWLSINKACPICYTEVFGD S R
Sbjct: 241 CGSKWLSINKACPICYTEVFGDISKR 244

BLAST of Tan0007960 vs. NCBI nr
Match: XP_022957965.1 (E3 ubiquitin ligase BIG BROTHER-like [Cucurbita moschata] >XP_022957966.1 E3 ubiquitin ligase BIG BROTHER-like [Cucurbita moschata] >XP_022957967.1 E3 ubiquitin ligase BIG BROTHER-like [Cucurbita moschata] >XP_022957968.1 E3 ubiquitin ligase BIG BROTHER-like [Cucurbita moschata] >KAG7035855.1 E3 ubiquitin ligase BIG BROTHER [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 421.8 bits (1083), Expect = 4.4e-114
Identity = 202/266 (75.94%), Postives = 212/266 (79.70%), Query Frame = 0

Query: 1   MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGPSHSQWVTVNLILCQYSKC 60
           MSWNSNIEVHCM NGYPYSTASFMEYFEGLTYDHVNFIFSGPSHSQ          Y K 
Sbjct: 1   MSWNSNIEVHCMTNGYPYSTASFMEYFEGLTYDHVNFIFSGPSHSQETVCPATNSSYYK- 60

Query: 61  IKLISQYGSIMFSFFHSGDCLSGHSDSWSTSCFDAQSLEVLGHESTIDEHRMPQDFSTIP 120
                                  H+DSWS SCFD  S EV GHES IDEHRM Q+FS +P
Sbjct: 61  ---------------------FEHADSWSASCFDTPSFEVQGHESAIDEHRMQQEFSPVP 120

Query: 121 NEQSLGNRVWEENANPNMAGNSMECPRRHSNYQDYQTIWQDIIDPDNMTYEELVDLGETV 180
           +EQSLGNR  EEN NP+MA NSMECPRRHSNY +YQTIWQDIIDPDNMTYEELVDLGETV
Sbjct: 121 DEQSLGNRAREENVNPDMASNSMECPRRHSNYHEYQTIWQDIIDPDNMTYEELVDLGETV 180

Query: 181 GTQSRGLSQELIALLPVSKYKCWFFSRKKSRNERCVICQMEYKRGDQRITLPCKHRYHTS 240
           G+QSRGLSQELIALLPVSK+KCWFFSRKKSR ERCVICQM YK GDQRITLPCKH+YHTS
Sbjct: 181 GSQSRGLSQELIALLPVSKFKCWFFSRKKSRTERCVICQMNYKHGDQRITLPCKHKYHTS 240

Query: 241 CGTKWLSINKACPICYTEVFGDNSNR 267
           CG+KWLSINKACPICYTEVFGD S R
Sbjct: 241 CGSKWLSINKACPICYTEVFGDISKR 244

BLAST of Tan0007960 vs. NCBI nr
Match: KAG6605908.1 (E3 ubiquitin-protein ligase BIG BROTHER, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 421.8 bits (1083), Expect = 4.4e-114
Identity = 202/266 (75.94%), Postives = 212/266 (79.70%), Query Frame = 0

Query: 1   MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGPSHSQWVTVNLILCQYSKC 60
           MSWNSNIEVHCM NGYPYSTASFMEYFEGLTYDHVNFIFSGPSHSQ          Y K 
Sbjct: 72  MSWNSNIEVHCMTNGYPYSTASFMEYFEGLTYDHVNFIFSGPSHSQETVCPATNSSYYK- 131

Query: 61  IKLISQYGSIMFSFFHSGDCLSGHSDSWSTSCFDAQSLEVLGHESTIDEHRMPQDFSTIP 120
                                  H+DSWS SCFD  S EV GHES IDEHRM Q+FS +P
Sbjct: 132 ---------------------FEHADSWSASCFDTPSFEVQGHESAIDEHRMQQEFSPVP 191

Query: 121 NEQSLGNRVWEENANPNMAGNSMECPRRHSNYQDYQTIWQDIIDPDNMTYEELVDLGETV 180
           +EQSLGNR  EEN NP+MA NSMECPRRHSNY +YQTIWQDIIDPDNMTYEELVDLGETV
Sbjct: 192 DEQSLGNRAREENVNPDMASNSMECPRRHSNYHEYQTIWQDIIDPDNMTYEELVDLGETV 251

Query: 181 GTQSRGLSQELIALLPVSKYKCWFFSRKKSRNERCVICQMEYKRGDQRITLPCKHRYHTS 240
           G+QSRGLSQELIALLPVSK+KCWFFSRKKSR ERCVICQM YK GDQRITLPCKH+YHTS
Sbjct: 252 GSQSRGLSQELIALLPVSKFKCWFFSRKKSRTERCVICQMNYKHGDQRITLPCKHKYHTS 311

Query: 241 CGTKWLSINKACPICYTEVFGDNSNR 267
           CG+KWLSINKACPICYTEVFGD S R
Sbjct: 312 CGSKWLSINKACPICYTEVFGDISKR 315

BLAST of Tan0007960 vs. ExPASy TrEMBL
Match: A0A6J1K714 (E3 ubiquitin ligase BIG BROTHER-like OS=Cucurbita maxima OX=3661 GN=LOC111490765 PE=4 SV=1)

HSP 1 Score: 425.6 bits (1093), Expect = 1.5e-115
Identity = 203/266 (76.32%), Postives = 215/266 (80.83%), Query Frame = 0

Query: 1   MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGPSHSQWVTVNLILCQYSKC 60
           MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFS PSHS+          Y K 
Sbjct: 1   MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSAPSHSEETVCPATNSSYYK- 60

Query: 61  IKLISQYGSIMFSFFHSGDCLSGHSDSWSTSCFDAQSLEVLGHESTIDEHRMPQDFSTIP 120
                                  H+DSWS SCFD QS EV GHES IDEHRM Q+FST+P
Sbjct: 61  ---------------------FEHADSWSASCFDTQSFEVQGHESVIDEHRMQQEFSTVP 120

Query: 121 NEQSLGNRVWEENANPNMAGNSMECPRRHSNYQDYQTIWQDIIDPDNMTYEELVDLGETV 180
           +EQSLGNRV EEN NP+MA NSMECPRRHSNY +YQTIWQDIIDPDNMTYEELVDLGETV
Sbjct: 121 DEQSLGNRVREENVNPDMASNSMECPRRHSNYHEYQTIWQDIIDPDNMTYEELVDLGETV 180

Query: 181 GTQSRGLSQELIALLPVSKYKCWFFSRKKSRNERCVICQMEYKRGDQRITLPCKHRYHTS 240
           G+QSRGLSQELIALLPVSK+KCWFFSRKKSR ERCVICQM YK GDQRITLPCKH+YHTS
Sbjct: 181 GSQSRGLSQELIALLPVSKFKCWFFSRKKSRTERCVICQMNYKHGDQRITLPCKHKYHTS 240

Query: 241 CGTKWLSINKACPICYTEVFGDNSNR 267
           CG+KWLSINKACPICYTEV+GD S R
Sbjct: 241 CGSKWLSINKACPICYTEVYGDISKR 244

BLAST of Tan0007960 vs. ExPASy TrEMBL
Match: A0A6J1H1S6 (E3 ubiquitin ligase BIG BROTHER-like OS=Cucurbita moschata OX=3662 GN=LOC111459339 PE=4 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 2.2e-114
Identity = 202/266 (75.94%), Postives = 212/266 (79.70%), Query Frame = 0

Query: 1   MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGPSHSQWVTVNLILCQYSKC 60
           MSWNSNIEVHCM NGYPYSTASFMEYFEGLTYDHVNFIFSGPSHSQ          Y K 
Sbjct: 1   MSWNSNIEVHCMTNGYPYSTASFMEYFEGLTYDHVNFIFSGPSHSQETVCPATNSSYYK- 60

Query: 61  IKLISQYGSIMFSFFHSGDCLSGHSDSWSTSCFDAQSLEVLGHESTIDEHRMPQDFSTIP 120
                                  H+DSWS SCFD  S EV GHES IDEHRM Q+FS +P
Sbjct: 61  ---------------------FEHADSWSASCFDTPSFEVQGHESAIDEHRMQQEFSPVP 120

Query: 121 NEQSLGNRVWEENANPNMAGNSMECPRRHSNYQDYQTIWQDIIDPDNMTYEELVDLGETV 180
           +EQSLGNR  EEN NP+MA NSMECPRRHSNY +YQTIWQDIIDPDNMTYEELVDLGETV
Sbjct: 121 DEQSLGNRAREENVNPDMASNSMECPRRHSNYHEYQTIWQDIIDPDNMTYEELVDLGETV 180

Query: 181 GTQSRGLSQELIALLPVSKYKCWFFSRKKSRNERCVICQMEYKRGDQRITLPCKHRYHTS 240
           G+QSRGLSQELIALLPVSK+KCWFFSRKKSR ERCVICQM YK GDQRITLPCKH+YHTS
Sbjct: 181 GSQSRGLSQELIALLPVSKFKCWFFSRKKSRTERCVICQMNYKHGDQRITLPCKHKYHTS 240

Query: 241 CGTKWLSINKACPICYTEVFGDNSNR 267
           CG+KWLSINKACPICYTEVFGD S R
Sbjct: 241 CGSKWLSINKACPICYTEVFGDISKR 244

BLAST of Tan0007960 vs. ExPASy TrEMBL
Match: A0A0A0L1Z5 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G290860 PE=4 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 5.0e-111
Identity = 202/266 (75.94%), Postives = 213/266 (80.08%), Query Frame = 0

Query: 1   MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGPSHSQWVTVNLILCQYSKC 60
           MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSG SHS          Q + C
Sbjct: 9   MSWNSNIEVHCMNNGYPYSTASFMEYFEGLTYDHVNFIFSGASHS----------QETVC 68

Query: 61  IKLISQYGSIMFSFFHSGDCLSGHSDSWSTSCFDAQSLEVLGHESTIDEHRMPQDFSTIP 120
               S Y               GHSD WS S FDAQS EV  HESTIDEHR P   ST+ 
Sbjct: 69  PSTNSSY-------------KFGHSDLWSASYFDAQSFEVQDHESTIDEHRRP--LSTVQ 128

Query: 121 NEQSLGNRVWEENANPNMAGNSMECPRRHSNYQDYQTIWQDIIDPDNMTYEELVDLGETV 180
           NEQS GN VWEENANPNM+GNSMECPRRHSNY +YQTIWQ+I+DPDNMTYEELVDLGETV
Sbjct: 129 NEQSSGNSVWEENANPNMSGNSMECPRRHSNYHEYQTIWQEIVDPDNMTYEELVDLGETV 188

Query: 181 GTQSRGLSQELIALLPVSKYKCWFFSRKKSRNERCVICQMEYKRGDQRITLPCKHRYHTS 240
           GTQSRGL+QELIALLPVS+Y+  FFSRKKSRNERCVICQMEYKRGD+ ITLPCKH+YHTS
Sbjct: 189 GTQSRGLTQELIALLPVSRYRWGFFSRKKSRNERCVICQMEYKRGDKIITLPCKHKYHTS 248

Query: 241 CGTKWLSINKACPICYTEVFGDNSNR 267
           CGTKWLSI KACPICYTEVFGD S R
Sbjct: 249 CGTKWLSIKKACPICYTEVFGDTSKR 249

BLAST of Tan0007960 vs. ExPASy TrEMBL
Match: A0A6J1D445 (E3 ubiquitin ligase BIG BROTHER-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017416 PE=4 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 6.5e-111
Identity = 201/269 (74.72%), Postives = 217/269 (80.67%), Query Frame = 0

Query: 1   MSWNSNIEVHCMNNGYPYSTA-SFMEYFEGLTYDHVNFIFSGPSHSQWVTVNLILCQYSK 60
           MSWN+++EV+CMNN YPYSTA SFMEYFEGLTY+HVNFIFSG SHSQ             
Sbjct: 13  MSWNTSMEVNCMNNSYPYSTAGSFMEYFEGLTYEHVNFIFSGASHSQETV---------- 72

Query: 61  CIKLISQYGSIMFSFFHSGDCLSGHSDSWSTSCFDAQSLEVLGHESTIDEHRMPQDFSTI 120
                  Y S   S++       GHSDSWS+S FDAQS EVLGHEST+DEHR PQDFST+
Sbjct: 73  -------YPSTNSSYYK-----FGHSDSWSSSYFDAQSSEVLGHESTVDEHRRPQDFSTL 132

Query: 121 PNEQSLGNRVWEENANPNMAG--NSMECPRRHSNYQDYQTIWQDIIDPDNMTYEELVDLG 180
           PNEQS GNR WEENANPNM+   N+MECPRRH NYQ+YQTIWQDI+DPDNMTYEEL+DLG
Sbjct: 133 PNEQSSGNRAWEENANPNMSSNTNTMECPRRHPNYQEYQTIWQDIVDPDNMTYEELLDLG 192

Query: 181 ETVGTQSRGLSQELIALLPVSKYKCWFFSRKKSRNERCVICQMEYKRGDQRITLPCKHRY 240
           ETVGT +RGL QELIALLPVSKYKC FF  KKSRNERCVICQMEYKRGDQRITLPCKHRY
Sbjct: 193 ETVGTHNRGLPQELIALLPVSKYKCGFFLMKKSRNERCVICQMEYKRGDQRITLPCKHRY 252

Query: 241 HTSCGTKWLSINKACPICYTEVFGDNSNR 267
           HT CGTKWLSINKACPICYTEVFG  S R
Sbjct: 253 HTCCGTKWLSINKACPICYTEVFGGTSKR 259

BLAST of Tan0007960 vs. ExPASy TrEMBL
Match: A0A6J1D6M7 (E3 ubiquitin ligase BIG BROTHER-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111017416 PE=4 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 6.5e-111
Identity = 201/269 (74.72%), Postives = 217/269 (80.67%), Query Frame = 0

Query: 1   MSWNSNIEVHCMNNGYPYSTA-SFMEYFEGLTYDHVNFIFSGPSHSQWVTVNLILCQYSK 60
           MSWN+++EV+CMNN YPYSTA SFMEYFEGLTY+HVNFIFSG SHSQ             
Sbjct: 1   MSWNTSMEVNCMNNSYPYSTAGSFMEYFEGLTYEHVNFIFSGASHSQETV---------- 60

Query: 61  CIKLISQYGSIMFSFFHSGDCLSGHSDSWSTSCFDAQSLEVLGHESTIDEHRMPQDFSTI 120
                  Y S   S++       GHSDSWS+S FDAQS EVLGHEST+DEHR PQDFST+
Sbjct: 61  -------YPSTNSSYYK-----FGHSDSWSSSYFDAQSSEVLGHESTVDEHRRPQDFSTL 120

Query: 121 PNEQSLGNRVWEENANPNMAG--NSMECPRRHSNYQDYQTIWQDIIDPDNMTYEELVDLG 180
           PNEQS GNR WEENANPNM+   N+MECPRRH NYQ+YQTIWQDI+DPDNMTYEEL+DLG
Sbjct: 121 PNEQSSGNRAWEENANPNMSSNTNTMECPRRHPNYQEYQTIWQDIVDPDNMTYEELLDLG 180

Query: 181 ETVGTQSRGLSQELIALLPVSKYKCWFFSRKKSRNERCVICQMEYKRGDQRITLPCKHRY 240
           ETVGT +RGL QELIALLPVSKYKC FF  KKSRNERCVICQMEYKRGDQRITLPCKHRY
Sbjct: 181 ETVGTHNRGLPQELIALLPVSKYKCGFFLMKKSRNERCVICQMEYKRGDQRITLPCKHRY 240

Query: 241 HTSCGTKWLSINKACPICYTEVFGDNSNR 267
           HT CGTKWLSINKACPICYTEVFG  S R
Sbjct: 241 HTCCGTKWLSINKACPICYTEVFGGTSKR 247

BLAST of Tan0007960 vs. TAIR 10
Match: AT3G63530.1 (RING/U-box superfamily protein )

HSP 1 Score: 158.7 bits (400), Expect = 6.5e-39
Identity = 97/258 (37.60%), Postives = 134/258 (51.94%), Query Frame = 0

Query: 8   EVHCMNNGYPY-STASFMEYFEGLTYDHVNFIFSGPSHSQWVTVNLILCQYSKCIKLISQ 67
           + H    G+PY +T S+M+++ G     +N+  +   H Q    NL     +   K    
Sbjct: 10  DAHYTETGFPYAATGSYMDFYGGAAQGPLNYDHAATMHPQ---DNLYWTMNTNAYK-FGF 69

Query: 68  YGSIMFSFFHSGDCLSGHSDSWSTSCFDAQSLEVLGHESTIDEHRMPQDFSTIPNEQSLG 127
            GS   SF+ S D ++ H    S               +  D H M        N  +  
Sbjct: 70  SGSDNASFYGSYD-MNDHLSRMSIG------------RTNWDYHPMVNVADDPENTVARS 129

Query: 128 NRVWEENANPNMAGNSMECPRRHSNYQDYQTIWQDIIDPDNMTYEELVDLGETVGTQSRG 187
            ++ + + +      + EC     +    Q  WQD IDPD MTYEELV+LGE VGT+SRG
Sbjct: 130 VQIGDTDEH----SEAEECIANEHDPDSPQVSWQDDIDPDTMTYEELVELGEAVGTESRG 189

Query: 188 LSQELIALLPVSKYKCWFFSRKKSRNERCVICQMEYKRGDQRITLPCKHRYHTSCGTKWL 247
           LSQELI  LP  KYK      +K   ERCVICQ++YK G++++ LPCKH YH+ C +KWL
Sbjct: 190 LSQELIETLPTKKYKFGSIFSRKRAGERCVICQLKYKIGERQMNLPCKHVYHSECISKWL 246

Query: 248 SINKACPICYTEVFGDNS 265
           SINK CP+C +EVFG+ S
Sbjct: 250 SINKVCPVCNSEVFGEPS 246

BLAST of Tan0007960 vs. TAIR 10
Match: AT3G63530.2 (RING/U-box superfamily protein )

HSP 1 Score: 158.7 bits (400), Expect = 6.5e-39
Identity = 97/258 (37.60%), Postives = 134/258 (51.94%), Query Frame = 0

Query: 8   EVHCMNNGYPY-STASFMEYFEGLTYDHVNFIFSGPSHSQWVTVNLILCQYSKCIKLISQ 67
           + H    G+PY +T S+M+++ G     +N+  +   H Q    NL     +   K    
Sbjct: 10  DAHYTETGFPYAATGSYMDFYGGAAQGPLNYDHAATMHPQ---DNLYWTMNTNAYK-FGF 69

Query: 68  YGSIMFSFFHSGDCLSGHSDSWSTSCFDAQSLEVLGHESTIDEHRMPQDFSTIPNEQSLG 127
            GS   SF+ S D ++ H    S               +  D H M        N  +  
Sbjct: 70  SGSDNASFYGSYD-MNDHLSRMSIG------------RTNWDYHPMVNVADDPENTVARS 129

Query: 128 NRVWEENANPNMAGNSMECPRRHSNYQDYQTIWQDIIDPDNMTYEELVDLGETVGTQSRG 187
            ++ + + +      + EC     +    Q  WQD IDPD MTYEELV+LGE VGT+SRG
Sbjct: 130 VQIGDTDEH----SEAEECIANEHDPDSPQVSWQDDIDPDTMTYEELVELGEAVGTESRG 189

Query: 188 LSQELIALLPVSKYKCWFFSRKKSRNERCVICQMEYKRGDQRITLPCKHRYHTSCGTKWL 247
           LSQELI  LP  KYK      +K   ERCVICQ++YK G++++ LPCKH YH+ C +KWL
Sbjct: 190 LSQELIETLPTKKYKFGSIFSRKRAGERCVICQLKYKIGERQMNLPCKHVYHSECISKWL 246

Query: 248 SINKACPICYTEVFGDNS 265
           SINK CP+C +EVFG+ S
Sbjct: 250 SINKVCPVCNSEVFGEPS 246

BLAST of Tan0007960 vs. TAIR 10
Match: AT3G19910.1 (RING/U-box superfamily protein )

HSP 1 Score: 114.0 bits (284), Expect = 1.8e-25
Identity = 53/111 (47.75%), Postives = 70/111 (63.06%), Query Frame = 0

Query: 156 QTIWQDIIDPDNMTYEELVDLGETVGTQSRGLSQELIALLPVSKYKCWFFSRKKSRNERC 215
           Q  W D +DPD ++YEEL+ LG+ VGT+SRGLS + IA LP  +YK      +   NE C
Sbjct: 232 QDAW-DEMDPDELSYEELLALGDIVGTESRGLSADTIASLPSKRYK--EGDNQNGTNESC 291

Query: 216 VICQMEYKRGDQRITLPCKHRYHTSCGTKWLSINKACPICYTEVFGDNSNR 267
           VIC+++Y+  +  I LPCKH YH+ C   WL INK CP+C  EV    S +
Sbjct: 292 VICRLDYEDDEDLILLPCKHSYHSECINNWLKINKVCPVCSAEVSTSTSGQ 339

BLAST of Tan0007960 vs. TAIR 10
Match: AT5G52140.1 (RING/U-box superfamily protein )

HSP 1 Score: 106.3 bits (264), Expect = 3.9e-23
Identity = 51/105 (48.57%), Postives = 66/105 (62.86%), Query Frame = 0

Query: 164 DPDNMTYEELVDLGETVGTQSRGLSQELIALLPVSKY----KCWFFSRKKSR----NERC 223
           DP+NMTYEEL +LG++VG   +GLSQE I+ L   KY    K W+    K +    + +C
Sbjct: 175 DPENMTYEELTELGDSVGDVGKGLSQERISRLRTHKYGTKTKSWYCLHMKKKFVADDSQC 234

Query: 224 VICQMEYKRGDQRITLPCKHRYHTSCGTKWLSINKACPICYTEVF 261
            IC MEY +GD+  TLPCKH YH  C ++WL  NK C IC  EV+
Sbjct: 235 SICLMEYAKGDKITTLPCKHIYHKDCISQWLKQNKVCCICKAEVY 279

BLAST of Tan0007960 vs. TAIR 10
Match: AT3G47180.1 (RING/U-box superfamily protein )

HSP 1 Score: 98.2 bits (243), Expect = 1.0e-20
Identity = 46/101 (45.54%), Postives = 65/101 (64.36%), Query Frame = 0

Query: 160 QDIIDPDNMTYEELVDLGETVGTQSRGLSQ-ELIALLPVSKYKCWFFSRKKSRNERCVIC 219
           +D IDPD ++YEEL+ LG+ +G ++RGL+  E+   L  S Y    FS  K+  +RCV+C
Sbjct: 109 EDEIDPDQLSYEELIALGDFIGVENRGLTPIEISTCLNASTY---VFSHNKNEIDRCVVC 168

Query: 220 QMEYKRGDQRITL-PCKHRYHTSCGTKWLSINKACPICYTE 259
           QME++  +  + L PC H YH+ C TKWL   K CPIC +E
Sbjct: 169 QMEFEERESLVVLRPCDHPYHSECITKWLETKKICPICCSE 206

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8L6499.2e-3837.60E3 ubiquitin-protein ligase BIG BROTHER OS=Arabidopsis thaliana OX=3702 GN=BB PE... [more]
Q9LT172.6e-2447.75E3 ubiquitin ligase BIG BROTHER-related OS=Arabidopsis thaliana OX=3702 GN=BBR P... [more]
O495001.3e-1546.39E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV... [more]
Q9ZQF98.4e-1544.00E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV... [more]
Q7XTV71.9e-1443.62Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=399... [more]
Match NameE-valueIdentityDescription
XP_038901510.11.3e-11678.95E3 ubiquitin-protein ligase BIG BROTHER-like [Benincasa hispida][more]
XP_022995132.13.1e-11576.32E3 ubiquitin ligase BIG BROTHER-like [Cucurbita maxima] >XP_022995133.1 E3 ubiqu... [more]
XP_023532670.11.5e-11476.32E3 ubiquitin ligase BIG BROTHER-like [Cucurbita pepo subsp. pepo] >XP_023532671.... [more]
XP_022957965.14.4e-11475.94E3 ubiquitin ligase BIG BROTHER-like [Cucurbita moschata] >XP_022957966.1 E3 ubi... [more]
KAG6605908.14.4e-11475.94E3 ubiquitin-protein ligase BIG BROTHER, partial [Cucurbita argyrosperma subsp. ... [more]
Match NameE-valueIdentityDescription
A0A6J1K7141.5e-11576.32E3 ubiquitin ligase BIG BROTHER-like OS=Cucurbita maxima OX=3661 GN=LOC111490765... [more]
A0A6J1H1S62.2e-11475.94E3 ubiquitin ligase BIG BROTHER-like OS=Cucurbita moschata OX=3662 GN=LOC1114593... [more]
A0A0A0L1Z55.0e-11175.94RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G290860 P... [more]
A0A6J1D4456.5e-11174.72E3 ubiquitin ligase BIG BROTHER-like isoform X1 OS=Momordica charantia OX=3673 G... [more]
A0A6J1D6M76.5e-11174.72E3 ubiquitin ligase BIG BROTHER-like isoform X2 OS=Momordica charantia OX=3673 G... [more]
Match NameE-valueIdentityDescription
AT3G63530.16.5e-3937.60RING/U-box superfamily protein [more]
AT3G63530.26.5e-3937.60RING/U-box superfamily protein [more]
AT3G19910.11.8e-2547.75RING/U-box superfamily protein [more]
AT5G52140.13.9e-2348.57RING/U-box superfamily protein [more]
AT3G47180.11.0e-2045.54RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 215..255
e-value: 9.3E-6
score: 35.1
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 214..255
e-value: 9.3E-11
score: 41.9
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 215..255
score: 10.969719
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 176..260
e-value: 4.9E-17
score: 63.4
IPR033276E3 ubiquitin-protein ligase BIG BROTHERPANTHERPTHR46400RING/U-BOX SUPERFAMILY PROTEINcoord: 1..264
NoneNo IPR availablePANTHERPTHR46400:SF5RING/U-BOX SUPERFAMILY PROTEINcoord: 1..264
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 205..260

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0007960.1Tan0007960.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046621 negative regulation of organ growth
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0004842 ubiquitin-protein transferase activity