Tan0007830 (gene) Snake gourd v1

Overview
NameTan0007830
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionmitogen-activated protein kinase kinase kinase 18-like
LocationLG08: 75028267 .. 75029855 (+)
RNA-Seq ExpressionTan0007830
SyntenyTan0007830
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTTTCGCTCCATAATCAATGATCCAGATCCACGCGCCTATATAAATTCTCCCTCAATTCCCGAATTCCCAAACCATTCTACTTTTCTTCCTCTGTTTCTTCCCTTTTTCCTCTGTTTCAGCTTTTTCGGCACTCAACAATGGCGAAAACGACCACGCCTTCGTGGACAAGAACTAACTGTGTCATCGGAAAGGGCTCCTTCGGAACAGTCAGTGTCGGAATCAGAAAATCGGACGGTCGGATCTTCGCGGTGAAGTCTGTGGAACAGAGTGTTGAATTCCGGCGGCAAATTGAGTGTTTAGAGAATGAAATTCGGGTTCTCCGATCCTTGAATTCGCCGTACGTGGTGGGGTTTCTCGGAGACGACGTTTCCGGGGTGTCTACGACGACGTCGTTTCGGAATCTGCACATGGAGTATTTGCCAGGTGGCACCGTCGCCGATGACCCAATTGTCGCCGGCGACGAGAAGGTTTTACGGGCGAGGACACGGTGTATTGTTTCGGCTCTGAGTTATATTCATTCGAAAGGAATTGTCCATTGTGATGTTAAAGGGGGGAATATTTTGATCGGATTGGATCCGAGTTTTGTGAAATTGGCCGATTTCGGCTCTGCACTTAGTGCCGGCGACACGTGTCAAGGACGGGTGGCGCCACGTGGGAGTCCGCTATGGATGGCGCCGGAGGTGGTTCGAGGGGAATACCAGGGGCCGGAATCGGATGTTTGGTCCGTCGGGTGTACGGTCATAGAAATGGTCACCGGGAAACCGGCGTGGGAAGATTTCGGGGCCGATACGCTGAGTCGAATCGGATTTTCCGACAAGGTTCCGGAGTTTCCGACGAGGTTGTCGGAACTCGGACGGGATTTTCTCCGGAAGTGTCTCAGAAGAGATCCGAGTGAGCGGTGGAGCTGTGATCGTCTGCTGCAGCATCCATTTCTGGCGGCGGCGGCGGCCTTGCCGGATTTCTCCGACGATAATTCCCCCCGGTGCGTGCTCGATTGGGTCAACGTCAATTTTGAGGACGACGAAGATGATGAAATCCCATTCTCCGGCGCAACCTCCGGTGACGGAGGCTGGGAAAGTGAGATCTCAGGCAACGGCAGAATTGGGAAATTGAGTACGAGTGAATGGCCGAATTGGGAATCGGATGGTTGGTCGTCGGTGAGAAGGTTCTGCAGTGAAGCGGCAGCGGCGCCGGCAAGCTGTGAAGAAGAAGAAGGGGTGGGGGTAGAATCGGAATATGGGAGTTTGAGAGGGGTAGAAGGGGTAATGGAGGGGACAAGTTCGGAATATTCGGAGTTTGTAAAGGACAGCCACGGAAAATTGGGAGGTGAATATTCAAATCCTGACGGTCTGGAAATTCCGGAGCGGCCACGTAATAATTGCGGCGGCGGCGGCAGAAGCGGCTGTGGCGGTGGCGGTGACGGTGGATTAAATTTCCGACGGTGGGGGTCTGAAAGATGTGAAATTATAACGATAACCTCGTGGATATATTCAATAGAATTGATATTATGTTATTATTGGAAAATATTAATGAAGCAATTGGTGTTATTTGGGAATTATAATTTATTACCTTTCTTTTGTGCCTAA

mRNA sequence

ATTTTTCGCTCCATAATCAATGATCCAGATCCACGCGCCTATATAAATTCTCCCTCAATTCCCGAATTCCCAAACCATTCTACTTTTCTTCCTCTGTTTCTTCCCTTTTTCCTCTGTTTCAGCTTTTTCGGCACTCAACAATGGCGAAAACGACCACGCCTTCGTGGACAAGAACTAACTGTGTCATCGGAAAGGGCTCCTTCGGAACAGTCAGTGTCGGAATCAGAAAATCGGACGGTCGGATCTTCGCGGTGAAGTCTGTGGAACAGAGTGTTGAATTCCGGCGGCAAATTGAGTGTTTAGAGAATGAAATTCGGGTTCTCCGATCCTTGAATTCGCCGTACGTGGTGGGGTTTCTCGGAGACGACGTTTCCGGGGTGTCTACGACGACGTCGTTTCGGAATCTGCACATGGAGTATTTGCCAGGTGGCACCGTCGCCGATGACCCAATTGTCGCCGGCGACGAGAAGGTTTTACGGGCGAGGACACGGTGTATTGTTTCGGCTCTGAGTTATATTCATTCGAAAGGAATTGTCCATTGTGATGTTAAAGGGGGGAATATTTTGATCGGATTGGATCCGAGTTTTGTGAAATTGGCCGATTTCGGCTCTGCACTTAGTGCCGGCGACACGTGTCAAGGACGGGTGGCGCCACGTGGGAGTCCGCTATGGATGGCGCCGGAGGTGGTTCGAGGGGAATACCAGGGGCCGGAATCGGATGTTTGGTCCGTCGGGTGTACGGTCATAGAAATGGTCACCGGGAAACCGGCGTGGGAAGATTTCGGGGCCGATACGCTGAGTCGAATCGGATTTTCCGACAAGGTTCCGGAGTTTCCGACGAGGTTGTCGGAACTCGGACGGGATTTTCTCCGGAAGTGTCTCAGAAGAGATCCGAGTGAGCGGTGGAGCTGTGATCGTCTGCTGCAGCATCCATTTCTGGCGGCGGCGGCGGCCTTGCCGGATTTCTCCGACGATAATTCCCCCCGGTGCGTGCTCGATTGGGTCAACGTCAATTTTGAGGACGACGAAGATGATGAAATCCCATTCTCCGGCGCAACCTCCGGTGACGGAGGCTGGGAAAGTGAGATCTCAGGCAACGGCAGAATTGGGAAATTGAGTACGAGTGAATGGCCGAATTGGGAATCGGATGGTTGGTCGTCGGTGAGAAGGTTCTGCAGTGAAGCGGCAGCGGCGCCGGCAAGCTGTGAAGAAGAAGAAGGGGTGGGGGTAGAATCGGAATATGGGAGTTTGAGAGGGGTAGAAGGGGTAATGGAGGGGACAAGTTCGGAATATTCGGAGTTTGTAAAGGACAGCCACGGAAAATTGGGAGGTGAATATTCAAATCCTGACGGTCTGGAAATTCCGGAGCGGCCACGTAATAATTGCGGCGGCGGCGGCAGAAGCGGCTGTGGCGGTGGCGGTGACGGTGGATTAAATTTCCGACGGTGGGGGTCTGAAAGATGTGAAATTATAACGATAACCTCGTGGATATATTCAATAGAATTGATATTATGTTATTATTGGAAAATATTAATGAAGCAATTGGTGTTATTTGGGAATTATAATTTATTACCTTTCTTTTGTGCCTAA

Coding sequence (CDS)

ATGGCGAAAACGACCACGCCTTCGTGGACAAGAACTAACTGTGTCATCGGAAAGGGCTCCTTCGGAACAGTCAGTGTCGGAATCAGAAAATCGGACGGTCGGATCTTCGCGGTGAAGTCTGTGGAACAGAGTGTTGAATTCCGGCGGCAAATTGAGTGTTTAGAGAATGAAATTCGGGTTCTCCGATCCTTGAATTCGCCGTACGTGGTGGGGTTTCTCGGAGACGACGTTTCCGGGGTGTCTACGACGACGTCGTTTCGGAATCTGCACATGGAGTATTTGCCAGGTGGCACCGTCGCCGATGACCCAATTGTCGCCGGCGACGAGAAGGTTTTACGGGCGAGGACACGGTGTATTGTTTCGGCTCTGAGTTATATTCATTCGAAAGGAATTGTCCATTGTGATGTTAAAGGGGGGAATATTTTGATCGGATTGGATCCGAGTTTTGTGAAATTGGCCGATTTCGGCTCTGCACTTAGTGCCGGCGACACGTGTCAAGGACGGGTGGCGCCACGTGGGAGTCCGCTATGGATGGCGCCGGAGGTGGTTCGAGGGGAATACCAGGGGCCGGAATCGGATGTTTGGTCCGTCGGGTGTACGGTCATAGAAATGGTCACCGGGAAACCGGCGTGGGAAGATTTCGGGGCCGATACGCTGAGTCGAATCGGATTTTCCGACAAGGTTCCGGAGTTTCCGACGAGGTTGTCGGAACTCGGACGGGATTTTCTCCGGAAGTGTCTCAGAAGAGATCCGAGTGAGCGGTGGAGCTGTGATCGTCTGCTGCAGCATCCATTTCTGGCGGCGGCGGCGGCCTTGCCGGATTTCTCCGACGATAATTCCCCCCGGTGCGTGCTCGATTGGGTCAACGTCAATTTTGAGGACGACGAAGATGATGAAATCCCATTCTCCGGCGCAACCTCCGGTGACGGAGGCTGGGAAAGTGAGATCTCAGGCAACGGCAGAATTGGGAAATTGAGTACGAGTGAATGGCCGAATTGGGAATCGGATGGTTGGTCGTCGGTGAGAAGGTTCTGCAGTGAAGCGGCAGCGGCGCCGGCAAGCTGTGAAGAAGAAGAAGGGGTGGGGGTAGAATCGGAATATGGGAGTTTGAGAGGGGTAGAAGGGGTAATGGAGGGGACAAGTTCGGAATATTCGGAGTTTGTAAAGGACAGCCACGGAAAATTGGGAGGTGAATATTCAAATCCTGACGGTCTGGAAATTCCGGAGCGGCCACGTAATAATTGCGGCGGCGGCGGCAGAAGCGGCTGTGGCGGTGGCGGTGACGGTGGATTAAATTTCCGACGGTGGGGGTCTGAAAGATGTGAAATTATAACGATAACCTCGTGGATATATTCAATAGAATTGATATTATGTTATTATTGGAAAATATTAATGAAGCAATTGGTGTTATTTGGGAATTATAATTTATTACCTTTCTTTTGTGCCTAA

Protein sequence

MAKTTTPSWTRTNCVIGKGSFGTVSVGIRKSDGRIFAVKSVEQSVEFRRQIECLENEIRVLRSLNSPYVVGFLGDDVSGVSTTTSFRNLHMEYLPGGTVADDPIVAGDEKVLRARTRCIVSALSYIHSKGIVHCDVKGGNILIGLDPSFVKLADFGSALSAGDTCQGRVAPRGSPLWMAPEVVRGEYQGPESDVWSVGCTVIEMVTGKPAWEDFGADTLSRIGFSDKVPEFPTRLSELGRDFLRKCLRRDPSERWSCDRLLQHPFLAAAAALPDFSDDNSPRCVLDWVNVNFEDDEDDEIPFSGATSGDGGWESEISGNGRIGKLSTSEWPNWESDGWSSVRRFCSEAAAAPASCEEEEGVGVESEYGSLRGVEGVMEGTSSEYSEFVKDSHGKLGGEYSNPDGLEIPERPRNNCGGGGRSGCGGGGDGGLNFRRWGSERCEIITITSWIYSIELILCYYWKILMKQLVLFGNYNLLPFFCA
Homology
BLAST of Tan0007830 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 2.7e-56
Identity = 131/309 (42.39%), Postives = 171/309 (55.34%), Query Frame = 0

Query: 9   WTRTNCVIGKGSFGTVSVGIRKSDGRIFAVKSVEQSVEFRRQIECLENEIRVLRSLNSPY 68
           WTR   ++G+GS  TV      +   I AVKS E       + E L+ E ++L SL+SPY
Sbjct: 3   WTRGR-ILGRGSTATVYAAAGHNSDEILAVKSSEV-----HRSEFLQREAKILSSLSSPY 62

Query: 69  VVGFLGDDVSGVSTTTSFRNLHMEYLPGGTVADDPIVAG---DEKVLRARTRCIVSALSY 128
           V+G+ G +    S      NL MEY P GT+ D     G   DE  +   TR I+  L Y
Sbjct: 63  VIGYRGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGRVDETRVVKYTRDILKGLEY 122

Query: 129 IHSKGIVHCDVKGGNILIGLDPSFVKLADFGSALSAGDTCQGRVAPRGSPLWMAPEVVRG 188
           IHSKGIVHCDVKG N++I  +    K+ADFG A       +  V   G+P +MAPEV RG
Sbjct: 123 IHSKGIVHCDVKGSNVVIS-EKGEAKIADFGCAKRVDPVFESPV--MGTPAFMAPEVARG 182

Query: 189 EYQGPESDVWSVGCTVIEMVTGKPAW-----EDFGADTLSRIGFSDKVPEFPTRLSELGR 248
           E QG ESD+W+VGCT+IEMVTG P W      +     L R+G+S + PE P  L+E  +
Sbjct: 183 EKQGKESDIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPELPCLLAEEAK 242

Query: 249 DFLRKCLRRDPSERWSCDRLLQHPFLAAAAALPDFSD-------DNSPRCVLD---WVNV 300
           DFL KCL+R+ +ERW+  +LL HPFL      PD           NSP  V D   W +V
Sbjct: 243 DFLEKCLKREANERWTATQLLNHPFLTTK---PDIEPVLVPGLISNSPTSVTDQTFWRSV 299

BLAST of Tan0007830 vs. ExPASy Swiss-Prot
Match: Q9ZVP5 (Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK18 PE=1 SV=1)

HSP 1 Score: 206.8 bits (525), Expect = 5.3e-52
Identity = 151/366 (41.26%), Postives = 191/366 (52.19%), Query Frame = 0

Query: 8   SWTRTNCVIGKGSFGTVSVGIRKSDGRIFAVKSVEQSVEFRRQIECLENEIRVLRSLNSP 67
           +WTR    +G+GS  TVS       G   AVK    S EF R  E L+ E ++L SLNSP
Sbjct: 2   NWTRGK-TLGRGSTATVSAATCHESGETLAVK----SAEFHRS-EFLQREAKILSSLNSP 61

Query: 68  YVVGFLGDDV-------SGVSTTTSFRNLHMEYLPGGTVADDPIVAG---DEKVLRARTR 127
           YV+G+ G ++       +G +TT S   L MEY P GT+ D     G   DE  +   TR
Sbjct: 62  YVIGYRGCEITREPFHNNGEATTYS---LLMEYAPYGTLTDVATKNGGFIDEARVVKYTR 121

Query: 128 CIVSALSYIH-SKGIVHCDVKGGNILIGLDPSFVKLADFGSALSAGDTCQGRVAPRGSPL 187
            I+  L YIH SKGI HCD+KG N+L+G +    K+ADFG A          V  RG+P 
Sbjct: 122 QILLGLEYIHNSKGIAHCDIKGSNVLVG-ENGEAKIADFGCAKWVEPEITEPV--RGTPA 181

Query: 188 WMAPEVVRGEYQGPESDVWSVGCTVIEMVTGKPAWEDFGAD------TLSRIGFSDKVPE 247
           +MAPE  RGE QG ESD+W+VGCTVIEMVTG   W   GAD       L R+G+  ++PE
Sbjct: 182 FMAPEAARGERQGKESDIWAVGCTVIEMVTGSQPW--IGADFTDPVSVLYRVGYLGELPE 241

Query: 248 FPTRLSELGRDFLRKCLRRDPSERWSCDRLLQHPFLA-AAAALPDFSDDNSPRCVLD--- 307
            P  L+E  +DFL KCL+++ +ERW+  +LL HPFL      L      NSP  V D   
Sbjct: 242 LPCSLTEQAKDFLGKCLKKEATERWTASQLLNHPFLVNKEPELVTGLVTNSPTSVTDQMF 301

Query: 308 WVNVNFEDDEDDEIPFSGATSGDGGWESEISGNGRIGKLSTSEW-------PNWESDG-- 344
           W +V  E  ED              WE     + RIG LS   W         W+ DG  
Sbjct: 302 WRSVEEEVSED----------RSSWWECH--EDERIGVLS---WIGHVVVESTWDLDGED 338

BLAST of Tan0007830 vs. ExPASy Swiss-Prot
Match: Q54R82 (Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=44689 GN=mkkA PE=1 SV=2)

HSP 1 Score: 184.5 bits (467), Expect = 2.8e-45
Identity = 94/261 (36.02%), Postives = 148/261 (56.70%), Query Frame = 0

Query: 15  VIGKGSFGTVSVGIRKSDGRIFAVKSVE-----QSVEFRRQIECLENEIRVLRSLNSPYV 74
           ++G+G +G+V +G+ K  G +FAVK +E        + +  I     EI V+RSL    +
Sbjct: 175 ILGRGGYGSVYLGLNKDTGELFAVKQLEIVDINSDPKLKNMILSFSKEIEVMRSLRHDNI 234

Query: 75  VGFLGDDVSGVSTTTSFRNLHMEYLPGGTVAD--DPIVAGDEKVLRARTRCIVSALSYIH 134
           V +L     G S   SF ++ +EY+PGG+++       A  E V++  T+ I+  LS++H
Sbjct: 235 VRYL-----GTSLDQSFLSVFLEYIPGGSISSLLGKFGAFSENVIKVYTKQILQGLSFLH 294

Query: 135 SKGIVHCDVKGGNILIGLDPSFVKLADFGSALSAGDTCQGRVAPRGSPLWMAPEVVRGEY 194
           +  I+H D+KG NILI      VKL+DFG + S         + +G+P WMAPEV++   
Sbjct: 295 ANSIIHRDIKGANILID-TKGIVKLSDFGCSKSFSGIVSQFKSMQGTPYWMAPEVIKQTG 354

Query: 195 QGPESDVWSVGCTVIEMVTGKPAWEDFG--ADTLSRIGFSDKVPEFPTRLSELGRDFLRK 254
            G  SD+WS+GC ++EM T +P W +    A  +  I  S+ +P  P+ +S+   DFL  
Sbjct: 355 HGRSSDIWSLGCVIVEMATAQPPWSNITELAAVMYHIASSNSIPNIPSHMSQEAFDFLNL 414

Query: 255 CLRRDPSERWSCDRLLQHPFL 267
           C +RDP ER   ++LL+HPF+
Sbjct: 415 CFKRDPKERPDANQLLKHPFI 429

BLAST of Tan0007830 vs. ExPASy Swiss-Prot
Match: Q40541 (Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097 GN=NPK1 PE=1 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 5.0e-42
Identity = 98/276 (35.51%), Postives = 151/276 (54.71%), Query Frame = 0

Query: 5   TTPSWTRTNCVIGKGSFGTVSVGIRKSDGRIFAVKSVEQSV------EFRRQIECLENEI 64
           T P   R   +IG G+FG V +G+    G + A+K V  ++        +  +  LE E+
Sbjct: 75  TPPIRWRKGEMIGCGAFGRVYMGMNVDSGELLAIKEVSIAMNGASRERAQAHVRELEEEV 134

Query: 65  RVLRSLNSPYVVGFLGDDVSGVSTTTSFRNLHMEYLPGGTVAD--DPIVAGDEKVLRART 124
            +L++L+ P +V +L     G +      N+ +E++PGG+++       +  E V+R  T
Sbjct: 135 NLLKNLSHPNIVRYL-----GTAREAGSLNILLEFVPGGSISSLLGKFGSFPESVIRMYT 194

Query: 125 RCIVSALSYIHSKGIVHCDVKGGNILIGLDPSFVKLADFGSALSAGD--TCQGRVAPRGS 184
           + ++  L Y+H  GI+H D+KG NIL+  +   +KLADFG++    +  T  G  + +G+
Sbjct: 195 KQLLLGLEYLHKNGIMHRDIKGANILVD-NKGCIKLADFGASKKVVELATMTGAKSMKGT 254

Query: 185 PLWMAPEVVRGEYQGPESDVWSVGCTVIEMVTGKPAWEDFGAD--TLSRIGFSDKVPEFP 244
           P WMAPEV+        +D+WSVGCT+IEM TGKP W     +   L  IG +   P  P
Sbjct: 255 PYWMAPEVILQTGHSFSADIWSVGCTIIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIP 314

Query: 245 TRLSELGRDFLRKCLRRDPSERWSCDRLLQHPFLAA 269
             LS   +DFL KCL+++P  R S   LLQHPF+ A
Sbjct: 315 EHLSAESKDFLLKCLQKEPHLRHSASNLLQHPFVTA 344

BLAST of Tan0007830 vs. ExPASy Swiss-Prot
Match: O22040 (Mitogen-activated protein kinase kinase kinase ANP1 OS=Arabidopsis thaliana OX=3702 GN=ANP1 PE=1 SV=2)

HSP 1 Score: 171.0 bits (432), Expect = 3.2e-41
Identity = 99/271 (36.53%), Postives = 152/271 (56.09%), Query Frame = 0

Query: 8   SWTRTNCVIGKGSFGTVSVGIRKSDGRIFAVKSVEQSVEF------RRQIECLENEIRVL 67
           SW R   +IG+G+FGTV +G+    G + AVK V  +  F      +  I+ LE E+++L
Sbjct: 68  SW-RKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLIAANFASKEKTQAHIQELEEEVKLL 127

Query: 68  RSLNSPYVVGFLGDDVSGVSTTTSFRNLHMEYLPGGTVAD--DPIVAGDEKVLRARTRCI 127
           ++L+ P +V +L     G        N+ +E++PGG+++   +      E V+R  TR +
Sbjct: 128 KNLSHPNIVRYL-----GTVREDDTLNILLEFVPGGSISSLLEKFGPFPESVVRTYTRQL 187

Query: 128 VSALSYIHSKGIVHCDVKGGNILIGLDPSFVKLADFGSALSAGD--TCQGRVAPRGSPLW 187
           +  L Y+H+  I+H D+KG NIL+  +   +KLADFG++    +  T  G  + +G+P W
Sbjct: 188 LLGLEYLHNHAIMHRDIKGANILVD-NKGCIKLADFGASKQVAELATMTGAKSMKGTPYW 247

Query: 188 MAPEVVRGEYQGPESDVWSVGCTVIEMVTGKPAWEDFGAD--TLSRIGFSDKVPEFPTRL 247
           MAPEV+        +D+WSVGCTVIEMVTGK  W     +   +  IG +   P  P  L
Sbjct: 248 MAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTKSHPPIPDTL 307

Query: 248 SELGRDFLRKCLRRDPSERWSCDRLLQHPFL 267
           S   +DFL KCL+  P+ R +   LL+HPF+
Sbjct: 308 SSDAKDFLLKCLQEVPNLRPTASELLKHPFV 331

BLAST of Tan0007830 vs. NCBI nr
Match: XP_022949062.1 (mitogen-activated protein kinase kinase kinase 18-like [Cucurbita moschata])

HSP 1 Score: 748.4 bits (1931), Expect = 3.8e-212
Identity = 382/482 (79.25%), Postives = 409/482 (84.85%), Query Frame = 0

Query: 1   MAKTTTPSWTRTNCVIGKGSFGTVSVGIRKSDGRIFAVKSVEQSVEFRRQIECLENEIRV 60
           MA TT  SWTR+NCV+GKGSFGTV+VGIR SDGR+FAVKSVEQSV FRRQIECLENEIR+
Sbjct: 1   MADTTKLSWTRSNCVVGKGSFGTVTVGIRNSDGRVFAVKSVEQSVGFRRQIECLENEIRI 60

Query: 61  LRSLNSPYVVGFLGDDVSGVSTTTSFRNLHMEYLPGGTVADDPIVAGDEKVLRARTRCIV 120
           LRSLNSPYVVGFLGDDVS  + TTS RNLHMEYLPGGTVADDPI  GDE VLRARTRCIV
Sbjct: 61  LRSLNSPYVVGFLGDDVSEETPTTSLRNLHMEYLPGGTVADDPIGTGDEMVLRARTRCIV 120

Query: 121 SALSYIHSKGIVHCDVKGGNILIGLDPSFVKLADFGSALSA--GDTCQGRVAPRGSPLWM 180
           SALS++HSKGIVHCDVKG N+LIGL+PSFVKLADFGSA+    GDTC+   APRGSPLWM
Sbjct: 121 SALSFVHSKGIVHCDVKGRNVLIGLNPSFVKLADFGSAIEIGDGDTCRCSGAPRGSPLWM 180

Query: 181 APEVVRGEYQGPESDVWSVGCTVIEMVTGKPAWEDFGADTLSRIGFSDKVPEFPTRLSEL 240
           APEVVRGEYQGPESDVWSVGCTVIEMVTGKPAWEDFGADTLSRIGFSD +PEFPTRLS L
Sbjct: 181 APEVVRGEYQGPESDVWSVGCTVIEMVTGKPAWEDFGADTLSRIGFSDDLPEFPTRLSGL 240

Query: 241 GRDFLRKCLRRDPSERWSCDRLLQHPFLAAAAALPDFSDDNSPRCVLDWVNVNFEDDEDD 300
           GRDFLRKCL RDP ERWSCDRLLQHPFLA  AA P  + +NSPRCVLDWVN +F+D+ED+
Sbjct: 241 GRDFLRKCLVRDPKERWSCDRLLQHPFLATVAASPQIAIENSPRCVLDWVNADFDDNEDE 300

Query: 301 EIPFSGATSGDGGWESEISGNGRIGKLSTSEWPNWESDGWSSVRRFCSEAAAAPASCEEE 360
           EIPFSG TSG G  ESEISG  RIGKLSTSEWPNWESDGWSS RRF     A P +C EE
Sbjct: 301 EIPFSGETSGAGSRESEISGKCRIGKLSTSEWPNWESDGWSSARRF----TATPVTC-EE 360

Query: 361 EGVGVESEYGSLRGVEGVMEGTSSEYSEFVKDSHGKLGGEYSNPDGLEIPERPRNNCGGG 420
           EGVGV  EYG+LRGVE  MEGTSSEYSEFVK S+ +LG   SNP GLE PERPRNNCG G
Sbjct: 361 EGVGVNWEYGNLRGVEMEMEGTSSEYSEFVKHSNEELGDALSNPGGLETPERPRNNCGSG 420

Query: 421 GRSGCGGGGDGGLNFRRWGSERCEIITITSWIYSIELILCYYWKILMKQLVLFGNYNLLP 480
             SG  GGG GGLN +RW S+  EII ITS IYSIELILCYYWKIL+K+LVLFG Y LL 
Sbjct: 421 --SGGDGGGGGGLNSQRWLSDNYEIIPITSLIYSIELILCYYWKILIKKLVLFGIYTLLL 475

BLAST of Tan0007830 vs. NCBI nr
Match: XP_023524561.1 (mitogen-activated protein kinase kinase kinase 18-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 744.2 bits (1920), Expect = 7.1e-211
Identity = 379/486 (77.98%), Postives = 407/486 (83.74%), Query Frame = 0

Query: 1   MAKTTTPSWTRTNCVIGKGSFGTVSVGIRKSDGRIFAVKSVEQSVEFRRQIECLENEIRV 60
           MA TT  SWTR+NCV+GKGSFGTV+VGIR SDGR+FAVKSVEQSV FRRQIECLENEIR+
Sbjct: 1   MADTTKLSWTRSNCVVGKGSFGTVTVGIRNSDGRVFAVKSVEQSVGFRRQIECLENEIRI 60

Query: 61  LRSLNSPYVVGFLGDDVSGVSTTTSFRNLHMEYLPGGTVADDPIVAGDEKVLRARTRCIV 120
           LRSLNSPYVVGFLGDDVS  + TTS RNLHMEYLPGGTVADDPI  GDE VLRARTRCIV
Sbjct: 61  LRSLNSPYVVGFLGDDVSDETPTTSLRNLHMEYLPGGTVADDPIGTGDEMVLRARTRCIV 120

Query: 121 SALSYIHSKGIVHCDVKGGNILIGLDPSFVKLADFGSALSA------GDTCQGRVAPRGS 180
           SAL Y+HSKGIVHCDVKG N+LIGL+PSFVKLADFGSA+        GDTC+  VAPRGS
Sbjct: 121 SALGYVHSKGIVHCDVKGRNVLIGLNPSFVKLADFGSAIEIGDGDGDGDTCRCSVAPRGS 180

Query: 181 PLWMAPEVVRGEYQGPESDVWSVGCTVIEMVTGKPAWEDFGADTLSRIGFSDKVPEFPTR 240
           PLWMAPEVVRGEYQGPESDVWSVGCTVIEMVTGKPAWEDFGADTLSRIGFSD +PEFPTR
Sbjct: 181 PLWMAPEVVRGEYQGPESDVWSVGCTVIEMVTGKPAWEDFGADTLSRIGFSDDLPEFPTR 240

Query: 241 LSELGRDFLRKCLRRDPSERWSCDRLLQHPFLAAAAALPDFSDDNSPRCVLDWVNVNFED 300
           LS LGRDFLRKCL RDP +RWSCDRLLQHPFLA  AA P  + +NSPRCVLDW+N +F+D
Sbjct: 241 LSGLGRDFLRKCLVRDPKKRWSCDRLLQHPFLATVAASPQIAIENSPRCVLDWINADFDD 300

Query: 301 DEDDEIPFSGATSGDGGWESEISGNGRIGKLSTSEWPNWESDGWSSVRRFCSEAAAAPAS 360
           +ED+EIPFSG TS  G  ESEISG  RIGKLSTSEWPNWESDGWSS RRF     A P +
Sbjct: 301 NEDEEIPFSGETSSAGNRESEISGKCRIGKLSTSEWPNWESDGWSSARRF----TATPVT 360

Query: 361 CEEEEGVGVESEYGSLRGVEGVMEGTSSEYSEFVKDSHGKLGGEYSNPDGLEIPERPRNN 420
           C EEEGVGV  EYG+ RGVE  MEG+SSEYSEFVK S  +LG   SNP GLE PERPRNN
Sbjct: 361 C-EEEGVGVNWEYGNSRGVEMEMEGSSSEYSEFVKPSDEELGAALSNPGGLETPERPRNN 420

Query: 421 CGGGGRSGCGGGGDGGLNFRRWGSERCEIITITSWIYSIELILCYYWKILMKQLVLFGNY 480
           CG G  SG GGGG GGLN +RW S+  EII ITS IYSIELILCYYWKIL+K+LVLFG Y
Sbjct: 421 CGSG--SGGGGGGGGGLNSQRWLSDNYEIIPITSLIYSIELILCYYWKILIKKLVLFGIY 479

BLAST of Tan0007830 vs. NCBI nr
Match: XP_038893928.1 (mitogen-activated protein kinase kinase kinase 18-like [Benincasa hispida])

HSP 1 Score: 743.0 bits (1917), Expect = 1.6e-210
Identity = 384/486 (79.01%), Postives = 412/486 (84.77%), Query Frame = 0

Query: 1   MAKTTTPSWTRTNCVIGKGSFGTVSVGIRKSDGRIFAVKSVEQSVEFRRQIECLENEIRV 60
           M KTT  SWTRT  VIGKGSFGTVSVGI KSDGRIFAVKSVEQS+ FRRQIE LENEIR+
Sbjct: 1   MVKTTPLSWTRTKSVIGKGSFGTVSVGIGKSDGRIFAVKSVEQSIGFRRQIESLENEIRI 60

Query: 61  LRSLNSPYVVGFLGDDVSGVSTTTSFRNLHMEYLPGGTVADDPIVAGDEKVLRARTRCIV 120
           LRSLNSPYVVGFLGDDVS  S TTSFRNLHMEYLPGGTVADD   AGD+K+LR RTRC+V
Sbjct: 61  LRSLNSPYVVGFLGDDVSNESPTTSFRNLHMEYLPGGTVADDATAAGDDKLLRERTRCLV 120

Query: 121 SALSYIHSKGIVHCDVKGGNILIGLDPSFVKLADFGSALS---AGDTCQGRVAPRGSPLW 180
           SALSYIHSKGIVHCDVKG NILIGL+P  VKLADFGSA+    AGD C G +APRGSPLW
Sbjct: 121 SALSYIHSKGIVHCDVKGRNILIGLNPRVVKLADFGSAIELSRAGDRCLGSIAPRGSPLW 180

Query: 181 MAPEVVRGEYQGPESDVWSVGCTVIEMVTGKPAWEDFGADTLSRIGFSDKVPEFPTRLSE 240
           MAPEVVRGE+QGPESDVWSVGCTVIEMVTGKPAWEDFGADTLSRIGFSD +P+FPTRLS+
Sbjct: 181 MAPEVVRGEFQGPESDVWSVGCTVIEMVTGKPAWEDFGADTLSRIGFSDDLPDFPTRLSD 240

Query: 241 LGRDFLRKCLRRDPSERWSCDRLLQHPFLAAAAALPDFSDDNSPRCVLDWVNVNFEDDED 300
           +GRDFLRKCLRR+PSERWSCDRLLQHPFLAAAA+ P+ + +NSPRCVLDWVN +F DDE 
Sbjct: 241 VGRDFLRKCLRRNPSERWSCDRLLQHPFLAAAAS-PEIAVENSPRCVLDWVNGSFSDDE- 300

Query: 301 DEIPFSGATSGDGGWESEISGNGRIGKLSTSEWPNWESDGWSSVRRFCSEAAA-APASC- 360
           +EIP +  TSG GG ESE+SG  RIGKLSTS WPNWESDGWS VR  CSEAAA   A C 
Sbjct: 301 EEIPIANETSGFGGQESELSGKERIGKLSTSVWPNWESDGWSPVRSCCSEAAAETEAICR 360

Query: 361 -EEEEGVGVESEYGSLRGVEGVMEGTSSEYSEFVKDSHGKLGGEYSNPDGLEIPERPRNN 420
            EEE+  G E EYG+LRGVEG MEGTSSEY EFV D+HGKLG EYSN  G++IPERPRNN
Sbjct: 361 EEEEDDEGAEWEYGNLRGVEGEMEGTSSEYLEFVADNHGKLGAEYSNHGGMKIPERPRNN 420

Query: 421 CGGGGRSGCGGGGDGGLNFRRWGSERCEIITITSWIYSIELILC--YYWKILMKQLVLFG 479
            GG   S   GGG GGL+FRRWGSER EI TITSW YSIE ILC  YYWKILMKQLVLFG
Sbjct: 421 YGG---SDGDGGGSGGLSFRRWGSERDEITTITSWKYSIEWILCYYYYWKILMKQLVLFG 480

BLAST of Tan0007830 vs. NCBI nr
Match: XP_022998673.1 (mitogen-activated protein kinase kinase kinase 18 [Cucurbita maxima])

HSP 1 Score: 740.3 bits (1910), Expect = 1.0e-209
Identity = 383/487 (78.64%), Postives = 407/487 (83.57%), Query Frame = 0

Query: 1   MAKTTTPSWTRTNCVIGKGSFGTVSVGIRKSDGRIFAVKSVEQSVEFRRQIECLENEIRV 60
           MA TT  SWTR+NCVIGKGSFGTV+VGIR SDGRIFAVKSV QSV FRRQIECLE EIR+
Sbjct: 1   MANTTKLSWTRSNCVIGKGSFGTVTVGIRNSDGRIFAVKSVGQSVGFRRQIECLEKEIRI 60

Query: 61  LRSLNSPYVVGFLGDDVSGVSTTTSFRNLHMEYLPGGTVADDPIVAGDEKVLRARTRCIV 120
           LRSLNSPYVVGFLGDDVS  +  TS RNLHMEYLPGGTVADDPI  GDE VLRARTRCIV
Sbjct: 61  LRSLNSPYVVGFLGDDVSDETPMTSLRNLHMEYLPGGTVADDPIGTGDEMVLRARTRCIV 120

Query: 121 SALSYIHSKGIVHCDVKGGNILIGLDPSFVKLADFGSALSA-------GDTCQGRVAPRG 180
           SALSY+HSKGIVHCDVKG N+LIGL+PSFVKLADFGSA+         GDTC+  VAPRG
Sbjct: 121 SALSYVHSKGIVHCDVKGRNVLIGLNPSFVKLADFGSAIEISGGDGGDGDTCRCSVAPRG 180

Query: 181 SPLWMAPEVVRGEYQGPESDVWSVGCTVIEMVTGKPAWEDFGADTLSRIGFSDKVPEFPT 240
           SPLWMAPEVVRGEYQGPESDVWSVGCTVIEMVTGKPAWEDFGADTLSRIGFSD +PEFPT
Sbjct: 181 SPLWMAPEVVRGEYQGPESDVWSVGCTVIEMVTGKPAWEDFGADTLSRIGFSDDLPEFPT 240

Query: 241 RLSELGRDFLRKCLRRDPSERWSCDRLLQHPFLAAAAALPDFSDDNSPRCVLDWVNVNFE 300
           RLS LG DFLRKCL RDP ERWSCDRLLQHPFLAA AA P+ + +NSPRCVLDWVN +F+
Sbjct: 241 RLSGLGLDFLRKCLVRDPKERWSCDRLLQHPFLAAVAASPEIAIENSPRCVLDWVNADFD 300

Query: 301 DDEDDEIPFSGATSGDGGWESEISGNGRIGKLSTSEWPNWESDGWSSVRRFCSEAAAAPA 360
           D+ED+EIPFSG TSG G  ESEISG  RIGKLSTSEWPNWESDGWSS RRF     AAP 
Sbjct: 301 DNEDEEIPFSGETSGAGSQESEISGKCRIGKLSTSEWPNWESDGWSSARRF----TAAPV 360

Query: 361 SCEEEEGVGVESEYGSLRGVEGVMEGTSSEYSEFVKDSHGKLGGEYSNPDGLEIPERPRN 420
           +C  EEGVGV  EY + RGVE  MEGTSSEYSEFVK S  +LG   SNP GLE PERPRN
Sbjct: 361 TC--EEGVGVNWEYENSRGVEMEMEGTSSEYSEFVKHSDEELGAALSNPSGLETPERPRN 420

Query: 421 NCGGGGRSGCGGGGDGGLNFRRWGSERCEIITITSWIYSIELILCYYWKILMKQLVLFGN 480
           NCG G  SG GGGG GGLN +RW S+  EIITITS IYSI+LILCYYWKILMK+LVLFG 
Sbjct: 421 NCGSG--SGSGGGG-GGLNSQRWLSDNYEIITITSLIYSIKLILCYYWKILMKKLVLFGI 478

BLAST of Tan0007830 vs. NCBI nr
Match: XP_008457561.1 (PREDICTED: mitogen-activated protein kinase kinase kinase NPK1 [Cucumis melo])

HSP 1 Score: 708.0 bits (1826), Expect = 5.6e-200
Identity = 375/501 (74.85%), Postives = 407/501 (81.24%), Query Frame = 0

Query: 1   MAKTTTPSWTRTNCVIGKGSFGTVSVGIRKSDGRIFAVKSVEQSVEFRRQIECLENEIRV 60
           M K+T  SWTR+N  IGKGSFGTVS+GI K   RIFAVKSVEQS  FR QIECLENEIR+
Sbjct: 1   MVKSTALSWTRSNSPIGKGSFGTVSLGIGKPHARIFAVKSVEQSHGFRPQIECLENEIRI 60

Query: 61  LRSLNSPYVVGFLGDDVSGVSTTTSFRNLHMEYLPGGTVADDPIVAGDEKVLRARTRCIV 120
           LRSLNSPYVV FLGDDVS  S TTSFRNLHMEYLPGGTVADDP   G+EK+LR RT C+V
Sbjct: 61  LRSLNSPYVVRFLGDDVSDESPTTSFRNLHMEYLPGGTVADDPTGTGNEKLLRERTWCLV 120

Query: 121 SALSYIHSKGIVHCDVKGGNILIGLDPSFVKLADFGSAL---SAGDTCQGRVAPRGSPLW 180
           SALSYIHSKGIVHCDVKG NIL+GL+P FVKLADFGSA+    AG+  +G VAPRGSPLW
Sbjct: 121 SALSYIHSKGIVHCDVKGRNILVGLNPGFVKLADFGSAIELHGAGERSRGSVAPRGSPLW 180

Query: 181 MAPEVVRGEYQGPESDVWSVGCTVIEMVTGKPAWEDFGADTLSRIGFSDKVPEFPTRLSE 240
           MAPEVVRGE+QGPESDVWSVGCTVIEMVTGKPAW+DFGADTLSRIGFSD +PEFPT LSE
Sbjct: 181 MAPEVVRGEFQGPESDVWSVGCTVIEMVTGKPAWDDFGADTLSRIGFSDDLPEFPTWLSE 240

Query: 241 LGRDFLRKCLRRDPSERWSCDRLLQHPFLAAAA--ALPDFSDDNSPRCVLDWVNVNFEDD 300
           + RDFLRKCLRR+PSERWSCDRLLQHPFLAAAA  A P+ + +NSPRCVLDWVN +F DD
Sbjct: 241 VCRDFLRKCLRRNPSERWSCDRLLQHPFLAAAAAEASPEIAVENSPRCVLDWVNSSFSDD 300

Query: 301 EDDEIPFSGATSGDGGWESEISGNGRIGKLSTSEWPNWESDGWSSVRRFCSEAAA-APAS 360
           E +EIP +   SG GG E+EI G  RIGKLSTSEWPNWESDGWS+VR  CSEAA     S
Sbjct: 301 E-EEIPNADEPSGSGGQENEIYGKERIGKLSTSEWPNWESDGWSAVRSCCSEAAGETEES 360

Query: 361 C----------EEEEGVGVESEYGSLRGVEGVMEGTSSEYSEFV-KDSHGKLGGEYSNPD 420
           C          EEEE  G E E G+ R VEG MEGTSSEYSEFV +D+HGKL  +YSNP 
Sbjct: 361 CRKDEEEEEEEEEEEEEGAEWECGNWRRVEGEMEGTSSEYSEFVRRDNHGKLDAQYSNPG 420

Query: 421 GLEIPERPRNNCGGGGRSGCG-GGGDGGLNFRRWGSERCEIITITSWIYSIELIL-CYYW 480
           G+ IPERPRNN G G   GCG GGG GGL+FRR G ER EI TITSWIYSIELIL CY+W
Sbjct: 421 GITIPERPRNNYGRG--DGCGDGGGSGGLSFRRLGYERYEITTITSWIYSIELILCCYFW 480

Query: 481 KILMKQLVLFGNYNLLPFFCA 483
            ILMKQLVLFGNY LLPFFCA
Sbjct: 481 HILMKQLVLFGNYTLLPFFCA 498

BLAST of Tan0007830 vs. ExPASy TrEMBL
Match: A0A6J1GAZ5 (mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita moschata OX=3662 GN=LOC111452526 PE=3 SV=1)

HSP 1 Score: 748.4 bits (1931), Expect = 1.8e-212
Identity = 382/482 (79.25%), Postives = 409/482 (84.85%), Query Frame = 0

Query: 1   MAKTTTPSWTRTNCVIGKGSFGTVSVGIRKSDGRIFAVKSVEQSVEFRRQIECLENEIRV 60
           MA TT  SWTR+NCV+GKGSFGTV+VGIR SDGR+FAVKSVEQSV FRRQIECLENEIR+
Sbjct: 1   MADTTKLSWTRSNCVVGKGSFGTVTVGIRNSDGRVFAVKSVEQSVGFRRQIECLENEIRI 60

Query: 61  LRSLNSPYVVGFLGDDVSGVSTTTSFRNLHMEYLPGGTVADDPIVAGDEKVLRARTRCIV 120
           LRSLNSPYVVGFLGDDVS  + TTS RNLHMEYLPGGTVADDPI  GDE VLRARTRCIV
Sbjct: 61  LRSLNSPYVVGFLGDDVSEETPTTSLRNLHMEYLPGGTVADDPIGTGDEMVLRARTRCIV 120

Query: 121 SALSYIHSKGIVHCDVKGGNILIGLDPSFVKLADFGSALSA--GDTCQGRVAPRGSPLWM 180
           SALS++HSKGIVHCDVKG N+LIGL+PSFVKLADFGSA+    GDTC+   APRGSPLWM
Sbjct: 121 SALSFVHSKGIVHCDVKGRNVLIGLNPSFVKLADFGSAIEIGDGDTCRCSGAPRGSPLWM 180

Query: 181 APEVVRGEYQGPESDVWSVGCTVIEMVTGKPAWEDFGADTLSRIGFSDKVPEFPTRLSEL 240
           APEVVRGEYQGPESDVWSVGCTVIEMVTGKPAWEDFGADTLSRIGFSD +PEFPTRLS L
Sbjct: 181 APEVVRGEYQGPESDVWSVGCTVIEMVTGKPAWEDFGADTLSRIGFSDDLPEFPTRLSGL 240

Query: 241 GRDFLRKCLRRDPSERWSCDRLLQHPFLAAAAALPDFSDDNSPRCVLDWVNVNFEDDEDD 300
           GRDFLRKCL RDP ERWSCDRLLQHPFLA  AA P  + +NSPRCVLDWVN +F+D+ED+
Sbjct: 241 GRDFLRKCLVRDPKERWSCDRLLQHPFLATVAASPQIAIENSPRCVLDWVNADFDDNEDE 300

Query: 301 EIPFSGATSGDGGWESEISGNGRIGKLSTSEWPNWESDGWSSVRRFCSEAAAAPASCEEE 360
           EIPFSG TSG G  ESEISG  RIGKLSTSEWPNWESDGWSS RRF     A P +C EE
Sbjct: 301 EIPFSGETSGAGSRESEISGKCRIGKLSTSEWPNWESDGWSSARRF----TATPVTC-EE 360

Query: 361 EGVGVESEYGSLRGVEGVMEGTSSEYSEFVKDSHGKLGGEYSNPDGLEIPERPRNNCGGG 420
           EGVGV  EYG+LRGVE  MEGTSSEYSEFVK S+ +LG   SNP GLE PERPRNNCG G
Sbjct: 361 EGVGVNWEYGNLRGVEMEMEGTSSEYSEFVKHSNEELGDALSNPGGLETPERPRNNCGSG 420

Query: 421 GRSGCGGGGDGGLNFRRWGSERCEIITITSWIYSIELILCYYWKILMKQLVLFGNYNLLP 480
             SG  GGG GGLN +RW S+  EII ITS IYSIELILCYYWKIL+K+LVLFG Y LL 
Sbjct: 421 --SGGDGGGGGGLNSQRWLSDNYEIIPITSLIYSIELILCYYWKILIKKLVLFGIYTLLL 475

BLAST of Tan0007830 vs. ExPASy TrEMBL
Match: A0A6J1KHE6 (mitogen-activated protein kinase kinase kinase 18 OS=Cucurbita maxima OX=3661 GN=LOC111493258 PE=3 SV=1)

HSP 1 Score: 740.3 bits (1910), Expect = 5.0e-210
Identity = 383/487 (78.64%), Postives = 407/487 (83.57%), Query Frame = 0

Query: 1   MAKTTTPSWTRTNCVIGKGSFGTVSVGIRKSDGRIFAVKSVEQSVEFRRQIECLENEIRV 60
           MA TT  SWTR+NCVIGKGSFGTV+VGIR SDGRIFAVKSV QSV FRRQIECLE EIR+
Sbjct: 1   MANTTKLSWTRSNCVIGKGSFGTVTVGIRNSDGRIFAVKSVGQSVGFRRQIECLEKEIRI 60

Query: 61  LRSLNSPYVVGFLGDDVSGVSTTTSFRNLHMEYLPGGTVADDPIVAGDEKVLRARTRCIV 120
           LRSLNSPYVVGFLGDDVS  +  TS RNLHMEYLPGGTVADDPI  GDE VLRARTRCIV
Sbjct: 61  LRSLNSPYVVGFLGDDVSDETPMTSLRNLHMEYLPGGTVADDPIGTGDEMVLRARTRCIV 120

Query: 121 SALSYIHSKGIVHCDVKGGNILIGLDPSFVKLADFGSALSA-------GDTCQGRVAPRG 180
           SALSY+HSKGIVHCDVKG N+LIGL+PSFVKLADFGSA+         GDTC+  VAPRG
Sbjct: 121 SALSYVHSKGIVHCDVKGRNVLIGLNPSFVKLADFGSAIEISGGDGGDGDTCRCSVAPRG 180

Query: 181 SPLWMAPEVVRGEYQGPESDVWSVGCTVIEMVTGKPAWEDFGADTLSRIGFSDKVPEFPT 240
           SPLWMAPEVVRGEYQGPESDVWSVGCTVIEMVTGKPAWEDFGADTLSRIGFSD +PEFPT
Sbjct: 181 SPLWMAPEVVRGEYQGPESDVWSVGCTVIEMVTGKPAWEDFGADTLSRIGFSDDLPEFPT 240

Query: 241 RLSELGRDFLRKCLRRDPSERWSCDRLLQHPFLAAAAALPDFSDDNSPRCVLDWVNVNFE 300
           RLS LG DFLRKCL RDP ERWSCDRLLQHPFLAA AA P+ + +NSPRCVLDWVN +F+
Sbjct: 241 RLSGLGLDFLRKCLVRDPKERWSCDRLLQHPFLAAVAASPEIAIENSPRCVLDWVNADFD 300

Query: 301 DDEDDEIPFSGATSGDGGWESEISGNGRIGKLSTSEWPNWESDGWSSVRRFCSEAAAAPA 360
           D+ED+EIPFSG TSG G  ESEISG  RIGKLSTSEWPNWESDGWSS RRF     AAP 
Sbjct: 301 DNEDEEIPFSGETSGAGSQESEISGKCRIGKLSTSEWPNWESDGWSSARRF----TAAPV 360

Query: 361 SCEEEEGVGVESEYGSLRGVEGVMEGTSSEYSEFVKDSHGKLGGEYSNPDGLEIPERPRN 420
           +C  EEGVGV  EY + RGVE  MEGTSSEYSEFVK S  +LG   SNP GLE PERPRN
Sbjct: 361 TC--EEGVGVNWEYENSRGVEMEMEGTSSEYSEFVKHSDEELGAALSNPSGLETPERPRN 420

Query: 421 NCGGGGRSGCGGGGDGGLNFRRWGSERCEIITITSWIYSIELILCYYWKILMKQLVLFGN 480
           NCG G  SG GGGG GGLN +RW S+  EIITITS IYSI+LILCYYWKILMK+LVLFG 
Sbjct: 421 NCGSG--SGSGGGG-GGLNSQRWLSDNYEIITITSLIYSIKLILCYYWKILMKKLVLFGI 478

BLAST of Tan0007830 vs. ExPASy TrEMBL
Match: A0A1S3C5Y2 (mitogen-activated protein kinase kinase kinase NPK1 OS=Cucumis melo OX=3656 GN=LOC103497226 PE=3 SV=1)

HSP 1 Score: 708.0 bits (1826), Expect = 2.7e-200
Identity = 375/501 (74.85%), Postives = 407/501 (81.24%), Query Frame = 0

Query: 1   MAKTTTPSWTRTNCVIGKGSFGTVSVGIRKSDGRIFAVKSVEQSVEFRRQIECLENEIRV 60
           M K+T  SWTR+N  IGKGSFGTVS+GI K   RIFAVKSVEQS  FR QIECLENEIR+
Sbjct: 1   MVKSTALSWTRSNSPIGKGSFGTVSLGIGKPHARIFAVKSVEQSHGFRPQIECLENEIRI 60

Query: 61  LRSLNSPYVVGFLGDDVSGVSTTTSFRNLHMEYLPGGTVADDPIVAGDEKVLRARTRCIV 120
           LRSLNSPYVV FLGDDVS  S TTSFRNLHMEYLPGGTVADDP   G+EK+LR RT C+V
Sbjct: 61  LRSLNSPYVVRFLGDDVSDESPTTSFRNLHMEYLPGGTVADDPTGTGNEKLLRERTWCLV 120

Query: 121 SALSYIHSKGIVHCDVKGGNILIGLDPSFVKLADFGSAL---SAGDTCQGRVAPRGSPLW 180
           SALSYIHSKGIVHCDVKG NIL+GL+P FVKLADFGSA+    AG+  +G VAPRGSPLW
Sbjct: 121 SALSYIHSKGIVHCDVKGRNILVGLNPGFVKLADFGSAIELHGAGERSRGSVAPRGSPLW 180

Query: 181 MAPEVVRGEYQGPESDVWSVGCTVIEMVTGKPAWEDFGADTLSRIGFSDKVPEFPTRLSE 240
           MAPEVVRGE+QGPESDVWSVGCTVIEMVTGKPAW+DFGADTLSRIGFSD +PEFPT LSE
Sbjct: 181 MAPEVVRGEFQGPESDVWSVGCTVIEMVTGKPAWDDFGADTLSRIGFSDDLPEFPTWLSE 240

Query: 241 LGRDFLRKCLRRDPSERWSCDRLLQHPFLAAAA--ALPDFSDDNSPRCVLDWVNVNFEDD 300
           + RDFLRKCLRR+PSERWSCDRLLQHPFLAAAA  A P+ + +NSPRCVLDWVN +F DD
Sbjct: 241 VCRDFLRKCLRRNPSERWSCDRLLQHPFLAAAAAEASPEIAVENSPRCVLDWVNSSFSDD 300

Query: 301 EDDEIPFSGATSGDGGWESEISGNGRIGKLSTSEWPNWESDGWSSVRRFCSEAAA-APAS 360
           E +EIP +   SG GG E+EI G  RIGKLSTSEWPNWESDGWS+VR  CSEAA     S
Sbjct: 301 E-EEIPNADEPSGSGGQENEIYGKERIGKLSTSEWPNWESDGWSAVRSCCSEAAGETEES 360

Query: 361 C----------EEEEGVGVESEYGSLRGVEGVMEGTSSEYSEFV-KDSHGKLGGEYSNPD 420
           C          EEEE  G E E G+ R VEG MEGTSSEYSEFV +D+HGKL  +YSNP 
Sbjct: 361 CRKDEEEEEEEEEEEEEGAEWECGNWRRVEGEMEGTSSEYSEFVRRDNHGKLDAQYSNPG 420

Query: 421 GLEIPERPRNNCGGGGRSGCG-GGGDGGLNFRRWGSERCEIITITSWIYSIELIL-CYYW 480
           G+ IPERPRNN G G   GCG GGG GGL+FRR G ER EI TITSWIYSIELIL CY+W
Sbjct: 421 GITIPERPRNNYGRG--DGCGDGGGSGGLSFRRLGYERYEITTITSWIYSIELILCCYFW 480

Query: 481 KILMKQLVLFGNYNLLPFFCA 483
            ILMKQLVLFGNY LLPFFCA
Sbjct: 481 HILMKQLVLFGNYTLLPFFCA 498

BLAST of Tan0007830 vs. ExPASy TrEMBL
Match: A0A0A0LVK2 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G532310 PE=3 SV=1)

HSP 1 Score: 683.3 bits (1762), Expect = 7.2e-193
Identity = 366/497 (73.64%), Postives = 400/497 (80.48%), Query Frame = 0

Query: 1   MAKTTTPSWTRTNCVIGKGSFGTVSVGIRKSDGRIFAVKSVEQSVEFRRQIECLENEIRV 60
           M KTT  SWTR+N  IGKGSF TVS+GIRK D RIFAVKSV+Q+   R QI+CLENEIR+
Sbjct: 1   MLKTTALSWTRSNSPIGKGSFATVSLGIRKPDARIFAVKSVQQTQTLRPQIDCLENEIRI 60

Query: 61  LRSLNSPYVVGFLGDDVSGVSTTTSFRNLHMEYLPGGTVADDPIVAGDEKVLRARTRCIV 120
           LRSLNSPYVV FLGDDVS  S TTSFRNLHMEYLPGGT ADDP    D+K+LR RT C+V
Sbjct: 61  LRSLNSPYVVAFLGDDVSHESPTTSFRNLHMEYLPGGTAADDPTGTRDDKLLRERTWCLV 120

Query: 121 SALSYIHSKGIVHCDVKGGNILIGLDPSFVKLADFGSAL---SAGDTCQGRVAPRGSPLW 180
           SALSYIHSKGIVHCDVKG N+LIGL+P F+KLADFGSA+     G   +  +APRGSPLW
Sbjct: 121 SALSYIHSKGIVHCDVKGRNVLIGLNPGFLKLADFGSAIELHGPGHRSRDSLAPRGSPLW 180

Query: 181 MAPEVVRGEYQGPESDVWSVGCTVIEMVTGKPAWEDFGADTLSRIGFSDKVPEFPTRLSE 240
           MAPEVVRGE+QGPESDVWS+GCTVIEMVTGKPAWEDFGADTLSRIGFSD +P+FPT LSE
Sbjct: 181 MAPEVVRGEFQGPESDVWSLGCTVIEMVTGKPAWEDFGADTLSRIGFSDDLPDFPTCLSE 240

Query: 241 LGRDFLRKCLRRDPSERWSCDRLLQHPFLAAAAAL---PDFSDDNSPRCVLDWVNVNFED 300
           + RDFLRKCLRR+PSERWSCDRLLQHPFLAAAAA    P  + +NSPRCVLDWVNV+F D
Sbjct: 241 VCRDFLRKCLRRNPSERWSCDRLLQHPFLAAAAAAAASPKIAVENSPRCVLDWVNVSFSD 300

Query: 301 DEDDEIPFSGATSGDGGWESEISGNGRIGKLS-TSEWPNWESDGWSSVRRFCSEAAA-AP 360
           DE +EIP +   SG GG E+EI G  RIGKLS TSEWPNWESDGWS+VR   SEAAA   
Sbjct: 301 DE-EEIPHADEASGSGGQENEIYGKERIGKLSTTSEWPNWESDGWSAVRSSYSEAAAETE 360

Query: 361 ASC----EEEEGVGVESEYGSLRGVEGVMEGTSSEYSEFV-KDSHGKLGGEYSNPDGLEI 420
           ASC    EEEEG G E E G+LR VEG MEG S EYSEFV +D+HGKLG EYSNP G+ I
Sbjct: 361 ASCRKEEEEEEGGGAEWECGNLRRVEGEMEGRSWEYSEFVRRDNHGKLGAEYSNPGGMII 420

Query: 421 PERPRNNCGGGGRSGCGGGGDGGLNFRRWGSERCEIIT-ITSWIYSIELIL-CYYWKILM 480
           PERPRNN GGGG  GC   G GGL+FRR G E  EI T ITSWIYSIELIL CYYW ILM
Sbjct: 421 PERPRNNFGGGG--GC---GSGGLSFRRLGYEISEITTIITSWIYSIELILCCYYWNILM 480

Query: 481 KQLVLFGNYNLLPFFCA 483
           K+LVLFGNY  LPFF A
Sbjct: 481 KKLVLFGNYTFLPFFSA 491

BLAST of Tan0007830 vs. ExPASy TrEMBL
Match: A0A5A7UY96 (Mitogen-activated protein kinase kinase kinase NPK1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold340G00810 PE=3 SV=1)

HSP 1 Score: 600.5 bits (1547), Expect = 6.1e-168
Identity = 311/411 (75.67%), Postives = 341/411 (82.97%), Query Frame = 0

Query: 1   MAKTTTPSWTRTNCVIGKGSFGTVSVGIRKSDGRIFAVKSVEQSVEFRRQIECLENEIRV 60
           M K+T  SWTR+N  IGKGSFGTVS+GI K   RIFAVKSVEQS  FR QIECLENEIR+
Sbjct: 1   MVKSTALSWTRSNSPIGKGSFGTVSLGIGKPHARIFAVKSVEQSHGFRPQIECLENEIRI 60

Query: 61  LRSLNSPYVVGFLGDDVSGVSTTTSFRNLHMEYLPGGTVADDPIVAGDEKVLRARTRCIV 120
           LRSLNSPYVV FLGDDVS  S TTSFRNLHMEYLPGGTVADDP   G+EK+LR RT C+V
Sbjct: 61  LRSLNSPYVVRFLGDDVSDESPTTSFRNLHMEYLPGGTVADDPTGTGNEKLLRERTWCLV 120

Query: 121 SALSYIHSKGIVHCDVKGGNILIGLDPSFVKLADFGSAL---SAGDTCQGRVAPRGSPLW 180
           SALSYIHSKGIVHCDVKG NIL+GL+P FVKLADFGSA+    AG+  +G VAPRGSPLW
Sbjct: 121 SALSYIHSKGIVHCDVKGRNILVGLNPGFVKLADFGSAIELHGAGERSRGSVAPRGSPLW 180

Query: 181 MAPEVVRGEYQGPESDVWSVGCTVIEMVTGKPAWEDFGADTLSRIGFSDKVPEFPTRLSE 240
           MAPEVVRGE+QGPESDVWSVGCTVIEMVTGKPAW+DFGADTLSRIGFSD +PEFPT LSE
Sbjct: 181 MAPEVVRGEFQGPESDVWSVGCTVIEMVTGKPAWDDFGADTLSRIGFSDDLPEFPTWLSE 240

Query: 241 LGRDFLRKCLRRDPSERWSCDRLLQHPFLAAAA--ALPDFSDDNSPRCVLDWVNVNFEDD 300
           + RDFLRKCLRR+PSERWSCDRLLQHPFLAAAA  A P+ + +NSPRCVLDWVN +F DD
Sbjct: 241 VCRDFLRKCLRRNPSERWSCDRLLQHPFLAAAAAEASPEIAVENSPRCVLDWVNSSFSDD 300

Query: 301 EDDEIPFSGATSGDGGWESEISGNGRIGKLSTSEWPNWESDGWSSVRRFCSEAAA-APAS 360
           E +EIP +   SG GG E+EI G  RIGKLSTSEWPNWESDGWS+VR  CSEAA     S
Sbjct: 301 E-EEIPNADEPSGSGGQENEIYGKERIGKLSTSEWPNWESDGWSAVRSCCSEAAGETEES 360

Query: 361 C--EEEEGVGVESEYGSLRGVEGVMEGTSSEYSEFV-KDSHGKLGGEYSNP 403
           C  +EEE  G E E G+ R VEG MEGTSSEYSEFV +D+HGKL  +YSNP
Sbjct: 361 CRKDEEEEEGAEWECGNWRRVEGEMEGTSSEYSEFVRRDNHGKLDAQYSNP 410

BLAST of Tan0007830 vs. TAIR 10
Match: AT2G30040.1 (mitogen-activated protein kinase kinase kinase 14 )

HSP 1 Score: 359.4 bits (921), Expect = 4.6e-99
Identity = 196/348 (56.32%), Postives = 242/348 (69.54%), Query Frame = 0

Query: 4   TTTPSWTRTNCVIGKGSFGTVSVGIRKSDGRIFAVKSVEQSVEFRRQIECLENEIRVLRS 63
           +++ SW R +CV G+G FGTVS  + K DG +FAVKS++ +     Q E LENEI +LRS
Sbjct: 12  SSSSSWIRGSCV-GRGCFGTVSKALSKIDGGLFAVKSIDLATCLPSQAESLENEIVILRS 71

Query: 64  LNS-PYVVGFLGDDVSGVSTTTSFRNLHMEYLPGGTVADDPIVAGDEKVLRARTRCIVSA 123
           + S P +V FLGDDVS    T SFRNLH+EY P G VA+  IV  +E +LR    C+VSA
Sbjct: 72  MKSHPNIVRFLGDDVS-KEGTASFRNLHLEYSPEGDVANGGIV--NETLLRRYVWCLVSA 131

Query: 124 LSYIHSKGIVHCDVKGGNILIGLDPSFVKLADFGSALSAGDTCQGRVAPRGSPLWMAPEV 183
           LS++HS GIVHCDVK  N+L+    S VKLADFGSA+   +     V+PRGSPLWMAPEV
Sbjct: 132 LSHVHSNGIVHCDVKSKNVLVFNGGSSVKLADFGSAVEF-EKSTIHVSPRGSPLWMAPEV 191

Query: 184 VRGEYQGPESDVWSVGCTVIEMVTGKPAWEDFGADTLSRIGFSDKVPEFPTRLSELGRDF 243
           VR EYQGPESDVWS+GCTVIEM+TGKPAWED G D+LSRIGFS+ +P  P  LSELGRDF
Sbjct: 192 VRREYQGPESDVWSLGCTVIEMLTGKPAWEDHGFDSLSRIGFSNDLPFIPVGLSELGRDF 251

Query: 244 LRKCLRRDPSERWSCDRLLQHPFLAAAAALPDFSDDNSPRCVLDWVNVNFEDDEDDEIPF 303
           L KCL+RD S+RWSCD+LLQHPFL        F  ++SPRCVLDWVN  F+++E+     
Sbjct: 252 LEKCLKRDRSQRWSCDQLLQHPFLCQDHH-DSFFTESSPRCVLDWVNSEFDEEEE----- 311

Query: 304 SGATSGDGGWESEISGNGRIGKLSTSEWPNWESDGWSSVRRFCSEAAA 351
               S +   ES +S   RI KL+ +   NWES+GW+ VR    E+ A
Sbjct: 312 ----SDEWRPESMVSAMARISKLAITGGANWESNGWTEVRDTSEESEA 344

BLAST of Tan0007830 vs. TAIR 10
Match: AT1G07150.1 (mitogen-activated protein kinase kinase kinase 13 )

HSP 1 Score: 354.0 bits (907), Expect = 1.9e-97
Identity = 198/375 (52.80%), Postives = 246/375 (65.60%), Query Frame = 0

Query: 1   MAKTTTPSWTRTNCVIGKGSFGTVSVGIRKSDGRIFAVKSVEQSVEFRRQIECLENEIRV 60
           M  + +  W R  C IG+G FG VS  I K++G +FAVKSV+ +     Q E LENEI V
Sbjct: 15  MLSSPSSFWVRGAC-IGRGCFGAVSTAISKTNGEVFAVKSVDLATSLPTQSESLENEISV 74

Query: 61  LRSLN-SPYVVGFLGDDVSGVSTTTSFRNLHMEYLPGGTVADDPIVAG----DEKVLRAR 120
            RSL   PY+V FLGD VS   TTT FRNL++EYLP G VA     AG    DE +L+  
Sbjct: 75  FRSLKPHPYIVKFLGDGVSKEGTTT-FRNLYLEYLPNGDVASHR--AGGKIEDETLLQRY 134

Query: 121 TRCIVSALSYIHSKGIVHCDVKGGNILIGLDPSFVKLADFGSALSAGDTCQGRVAPRGSP 180
           T C+VSAL ++HS+G VHCDVK  NIL+    S VKLADFGSA     T +  + PRGSP
Sbjct: 135 TACLVSALRHVHSQGFVHCDVKARNILVS-QSSMVKLADFGSAFRI-HTPRALITPRGSP 194

Query: 181 LWMAPEVVRGEYQGPESDVWSVGCTVIEMVTGKPAWEDFGADTLSRIGFSDKVPEFPTRL 240
           LWMAPEV+R EYQGPESDVWS+GCT+IEM TGKPAWED G D+LSRI FSD++P FP++L
Sbjct: 195 LWMAPEVIRREYQGPESDVWSLGCTIIEMFTGKPAWEDHGIDSLSRISFSDELPVFPSKL 254

Query: 241 SELGRDFLRKCLRRDPSERWSCDRLLQHPFLAAAAALPDFSDDNSPRCVLDWVNVNFE-D 300
           SE+GRDFL KCL+RDP++RWSCD+LLQHPFL+          ++SPRCVLDWVN  F+ +
Sbjct: 255 SEIGRDFLEKCLKRDPNQRWSCDQLLQHPFLSQCH--NSSPTESSPRCVLDWVNSGFDLE 314

Query: 301 DEDDEIPFSGATSGDGGWESEISGNGRIGKLSTSEWPNWESDGWSSVRRFCSEAAAAPAS 360
           +E++E+         G  E E +    I  L+T+    WESDGW  VR  C  +     +
Sbjct: 315 EEEEEV---------GRSEFEDAAKAIICNLATTGGVIWESDGWVEVR--CHASEEEGTT 370

Query: 361 CEEEEGVGVESEYGS 370
            E      VESEY +
Sbjct: 375 MEYSGSTRVESEYNT 370

BLAST of Tan0007830 vs. TAIR 10
Match: AT1G07150.2 (mitogen-activated protein kinase kinase kinase 13 )

HSP 1 Score: 354.0 bits (907), Expect = 1.9e-97
Identity = 198/375 (52.80%), Postives = 246/375 (65.60%), Query Frame = 0

Query: 1   MAKTTTPSWTRTNCVIGKGSFGTVSVGIRKSDGRIFAVKSVEQSVEFRRQIECLENEIRV 60
           M  + +  W R  C IG+G FG VS  I K++G +FAVKSV+ +     Q E LENEI V
Sbjct: 1   MLSSPSSFWVRGAC-IGRGCFGAVSTAISKTNGEVFAVKSVDLATSLPTQSESLENEISV 60

Query: 61  LRSLN-SPYVVGFLGDDVSGVSTTTSFRNLHMEYLPGGTVADDPIVAG----DEKVLRAR 120
            RSL   PY+V FLGD VS   TTT FRNL++EYLP G VA     AG    DE +L+  
Sbjct: 61  FRSLKPHPYIVKFLGDGVSKEGTTT-FRNLYLEYLPNGDVASHR--AGGKIEDETLLQRY 120

Query: 121 TRCIVSALSYIHSKGIVHCDVKGGNILIGLDPSFVKLADFGSALSAGDTCQGRVAPRGSP 180
           T C+VSAL ++HS+G VHCDVK  NIL+    S VKLADFGSA     T +  + PRGSP
Sbjct: 121 TACLVSALRHVHSQGFVHCDVKARNILVS-QSSMVKLADFGSAFRI-HTPRALITPRGSP 180

Query: 181 LWMAPEVVRGEYQGPESDVWSVGCTVIEMVTGKPAWEDFGADTLSRIGFSDKVPEFPTRL 240
           LWMAPEV+R EYQGPESDVWS+GCT+IEM TGKPAWED G D+LSRI FSD++P FP++L
Sbjct: 181 LWMAPEVIRREYQGPESDVWSLGCTIIEMFTGKPAWEDHGIDSLSRISFSDELPVFPSKL 240

Query: 241 SELGRDFLRKCLRRDPSERWSCDRLLQHPFLAAAAALPDFSDDNSPRCVLDWVNVNFE-D 300
           SE+GRDFL KCL+RDP++RWSCD+LLQHPFL+          ++SPRCVLDWVN  F+ +
Sbjct: 241 SEIGRDFLEKCLKRDPNQRWSCDQLLQHPFLSQCH--NSSPTESSPRCVLDWVNSGFDLE 300

Query: 301 DEDDEIPFSGATSGDGGWESEISGNGRIGKLSTSEWPNWESDGWSSVRRFCSEAAAAPAS 360
           +E++E+         G  E E +    I  L+T+    WESDGW  VR  C  +     +
Sbjct: 301 EEEEEV---------GRSEFEDAAKAIICNLATTGGVIWESDGWVEVR--CHASEEEGTT 356

Query: 361 CEEEEGVGVESEYGS 370
            E      VESEY +
Sbjct: 361 MEYSGSTRVESEYNT 356

BLAST of Tan0007830 vs. TAIR 10
Match: AT2G32510.1 (mitogen-activated protein kinase kinase kinase 17 )

HSP 1 Score: 221.1 bits (562), Expect = 1.9e-57
Identity = 131/309 (42.39%), Postives = 171/309 (55.34%), Query Frame = 0

Query: 9   WTRTNCVIGKGSFGTVSVGIRKSDGRIFAVKSVEQSVEFRRQIECLENEIRVLRSLNSPY 68
           WTR   ++G+GS  TV      +   I AVKS E       + E L+ E ++L SL+SPY
Sbjct: 3   WTRGR-ILGRGSTATVYAAAGHNSDEILAVKSSEV-----HRSEFLQREAKILSSLSSPY 62

Query: 69  VVGFLGDDVSGVSTTTSFRNLHMEYLPGGTVADDPIVAG---DEKVLRARTRCIVSALSY 128
           V+G+ G +    S      NL MEY P GT+ D     G   DE  +   TR I+  L Y
Sbjct: 63  VIGYRGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGRVDETRVVKYTRDILKGLEY 122

Query: 129 IHSKGIVHCDVKGGNILIGLDPSFVKLADFGSALSAGDTCQGRVAPRGSPLWMAPEVVRG 188
           IHSKGIVHCDVKG N++I  +    K+ADFG A       +  V   G+P +MAPEV RG
Sbjct: 123 IHSKGIVHCDVKGSNVVIS-EKGEAKIADFGCAKRVDPVFESPV--MGTPAFMAPEVARG 182

Query: 189 EYQGPESDVWSVGCTVIEMVTGKPAW-----EDFGADTLSRIGFSDKVPEFPTRLSELGR 248
           E QG ESD+W+VGCT+IEMVTG P W      +     L R+G+S + PE P  L+E  +
Sbjct: 183 EKQGKESDIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPELPCLLAEEAK 242

Query: 249 DFLRKCLRRDPSERWSCDRLLQHPFLAAAAALPDFSD-------DNSPRCVLD---WVNV 300
           DFL KCL+R+ +ERW+  +LL HPFL      PD           NSP  V D   W +V
Sbjct: 243 DFLEKCLKREANERWTATQLLNHPFLTTK---PDIEPVLVPGLISNSPTSVTDQTFWRSV 299

BLAST of Tan0007830 vs. TAIR 10
Match: AT5G55090.1 (mitogen-activated protein kinase kinase kinase 15 )

HSP 1 Score: 210.7 bits (535), Expect = 2.6e-54
Identity = 147/385 (38.18%), Postives = 210/385 (54.55%), Query Frame = 0

Query: 8   SWTRTNCVIGKGSFGTVSVGIRKSDGRIFAVKSVEQSVEFRRQIECLENEIRVLRSLNSP 67
           +W R   +IG+GS  TVS+GI  S G  FAVKS E S         L+ E  +L  L+SP
Sbjct: 5   NWIR-GPIIGRGSTATVSLGITNS-GDFFAVKSAEFS-----SSAFLQREQSILSKLSSP 64

Query: 68  YVVGFLGDDVSGVSTTTSFRNLHMEYLPGGTVADDPIVAG---DEKVLRARTRCIVSALS 127
           Y+V ++G +V+  +    + NL MEY+ GG++ D    +G    E ++R+ TR I+  L 
Sbjct: 65  YIVKYIGSNVTKENDKLMY-NLLMEYVSGGSLHDLIKNSGGKLPEPLIRSYTRQILKGLM 124

Query: 128 YIHSKGIVHCDVKGGNILIGLDPSFVKLADFGSALSAGDTCQGRVAPRGSPLWMAPEVVR 187
           Y+H +GIVHCDVK  N++IG      K+ D G A +  +     +   G+P +M+PEV R
Sbjct: 125 YLHDQGIVHCDVKSQNVMIG--GEIAKIVDLGCAKTVEE--NENLEFSGTPAFMSPEVAR 184

Query: 188 GEYQGPESDVWSVGCTVIEMVTGKPAWEDFG--ADTLSRIGFSDKVPEFPTRLSELGRDF 247
           GE Q   +DVW++GCTVIEM TG   W +       + +IGF+ + P  P  LSE G+DF
Sbjct: 185 GEEQSFPADVWALGCTVIEMATGSSPWPELNDVVAAIYKIGFTGESPVIPVWLSEKGQDF 244

Query: 248 LRKCLRRDPSERWSCDRLLQHPFLAAAAALPDFS----DDNSPRCVLD---W----VNVN 307
           LRKCLR+DP +RW+ + LLQHPFL       D +    + +SP  VLD   W     + +
Sbjct: 245 LRKCLRKDPKQRWTVEELLQHPFLDEEDNDSDQTGNCLNSSSPSTVLDQRFWDLCETSRS 304

Query: 308 FEDDEDDEIPFSGATSGDGGWESEISGNGRIGKLS---TSEWPNWESDGWSSVR------ 363
               ED E PF+ +T  +  W+ +     RI KL+   +S  P+WE++GW  VR      
Sbjct: 305 RFIKEDHEDPFANST--NFLWDDDSLPGDRIKKLAGDESSGEPDWETNGWIEVRGEIEKR 364

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O808882.7e-5642.39Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370... [more]
Q9ZVP55.3e-5241.26Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=370... [more]
Q54R822.8e-4536.02Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=... [more]
Q405415.0e-4235.51Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097... [more]
O220403.2e-4136.53Mitogen-activated protein kinase kinase kinase ANP1 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
XP_022949062.13.8e-21279.25mitogen-activated protein kinase kinase kinase 18-like [Cucurbita moschata][more]
XP_023524561.17.1e-21177.98mitogen-activated protein kinase kinase kinase 18-like [Cucurbita pepo subsp. pe... [more]
XP_038893928.11.6e-21079.01mitogen-activated protein kinase kinase kinase 18-like [Benincasa hispida][more]
XP_022998673.11.0e-20978.64mitogen-activated protein kinase kinase kinase 18 [Cucurbita maxima][more]
XP_008457561.15.6e-20074.85PREDICTED: mitogen-activated protein kinase kinase kinase NPK1 [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A6J1GAZ51.8e-21279.25mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita moschata OX=... [more]
A0A6J1KHE65.0e-21078.64mitogen-activated protein kinase kinase kinase 18 OS=Cucurbita maxima OX=3661 GN... [more]
A0A1S3C5Y22.7e-20074.85mitogen-activated protein kinase kinase kinase NPK1 OS=Cucumis melo OX=3656 GN=L... [more]
A0A0A0LVK27.2e-19373.64Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G532... [more]
A0A5A7UY966.1e-16875.67Mitogen-activated protein kinase kinase kinase NPK1 OS=Cucumis melo var. makuwa ... [more]
Match NameE-valueIdentityDescription
AT2G30040.14.6e-9956.32mitogen-activated protein kinase kinase kinase 14 [more]
AT1G07150.11.9e-9752.80mitogen-activated protein kinase kinase kinase 13 [more]
AT1G07150.21.9e-9752.80mitogen-activated protein kinase kinase kinase 13 [more]
AT2G32510.11.9e-5742.39mitogen-activated protein kinase kinase kinase 17 [more]
AT5G55090.12.6e-5438.18mitogen-activated protein kinase kinase kinase 15 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 44..64
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 8..294
e-value: 1.9E-63
score: 216.6
NoneNo IPR availablePIRSRPIRSR037014-1PIRSR037014-1coord: 15..272
e-value: 2.4E-18
score: 63.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 296..329
NoneNo IPR availablePANTHERPTHR48011:SF7MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 14coord: 4..367
NoneNo IPR availablePANTHERPTHR48011CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATEDcoord: 4..367
NoneNo IPR availableCDDcd06606STKc_MAPKKKcoord: 8..265
e-value: 3.54227E-117
score: 342.578
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 10..266
e-value: 5.4E-67
score: 238.6
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 15..266
e-value: 9.4E-53
score: 179.2
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 10..266
score: 38.534245
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 16..39
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 131..143
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 14..280

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0007830.1Tan0007830.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009658 chloroplast organization
biological_process GO:0045893 positive regulation of transcription, DNA-templated
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0019901 protein kinase binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity