Tan0006710 (gene) Snake gourd v1

Overview


Name	Tan0006710
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Pectinesterase
Location	LG01: 59089739 .. 59091361 (-)
RNA-Seq Expression	Tan0006710
Synteny	Tan0006710

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCATTTCAACTACATGTGTTCTTTATAGTTAGCTTACTAATTTTCATCAATCCTTTTGTTGAATTTTCTTATAGCTTTATGTTTAATTTAACCGTGTCTTTAGATGGTTATGGTAATTTTGCTCGAATTAGCGATGCTATTGCAGCAGCACCAAACTTCAGCACTACTAGATTTTATATTCACGTGAAACCTGGTATTTATCACGAGTATGTTGAAGTTCCAATTCAAAAGACATTCATTGCTTTGATTGGAGATGATGCTTCTACAACCATCATCGTTGGTAATCGAAGTAATGACACTGGATTTTCAACTCCAACAACTGCAACCCTCAGTAAGTCTTTTCTATTCTACTAAACCCATGTTTGGAAGTGATTTTGAAATAGTTAGAATCACTTTTGTCGCACTCAAATTTCACTCTCGAACATGCTTAGAATGAAAACTGCATTAAAAAGTATAAAATCAAACCTCAAAGCTTCGTTTGATAATCATTTGGTTTTTGAAAATTAAGCTTGTTTTCTTCTAATTTCTCTACAATGGTTTTTATCACCTTAAGAAGCATTTTAAATTCTTAGCTAAATTTTAAAAACAAAAACAAGTTTTCAAAACTTGCTTTTTTTTTCTTTCTTTTTTTTGGTTTTTGACATATGGCTTGGTTTTTGAAAAAATATATAAAAAATAGATAACAAAATAACGTGGAAAAAAGACAAAAAACCAAAAAACAAATGGTTATCAAACGGGACCTAATTAATTAGAATTTGAATGACTAAAAGCATGTTTAGGAATAATTTTAAACATGAAAAACGTGATTTTGAGCTCCCATATATACATATATGCCCAAACAAAACAAAGTTGACATGTCCATATTAATTATATCCTGCACATTTTGTTCTAGAAATTATGAAAATAAAGCTAAAAAATGTATTGTTTGCAATTGTAGGTGTGAAAGGTGAAAACTTCATGGCTAAGTTGTTGACTTTTGAAAACTCTGCTGGACCAAAGAAAGGGCAAGCAGTGGCTGTTTTCAACATGGCGAACTACACATCGTACTACAAATGTGTATTTTTAGGTTATCAAGACACCCTTTATGCAGGAGCATTACCACAATTCTTCAAAGAGTGCGACATATACGGAACCGTAGACTTTATTTTCGGAAACGGACTTGCAGTATTCCAAGACTGTAATGTATACGCTCGATTACCCGACATTCAAATTACAATCACTGCACAATCCAAAGACAACATAAACAGTTTGAGTGGCTTCGTGTTCCAAAACTGCAATGTGACAGTGTCACCAGACATAACATCAAGCAAGGACAATGTTACTGTGTTCCTTGGACGACCATGGAGGAAATATTCGATGGTCGTTTTCATGGAGTCGTTTCTTGACAACGTAGTTCAACCAAGAGGATGGTTGGAGTGGCCAGGTGAGCCATTGAATTTACTATTTTATGCTGAATTTAATAATAATGGTCCAGGAGCTGACACTTCTCGAAGAGTCAATTGGCCTACTTACCATGTATTGCATAATACAATGGATGTTGCAAAGTTTACGGTTGGAAATTTCATTGATGGGACTAAATGGCTACCCCAAACTGGCATCCCTTTTAGGACTGGCTTGTGA

mRNA sequence

ATGGCATTTCAACTACATGTGTTCTTTATAGTTAGCTTACTAATTTTCATCAATCCTTTTGTTGAATTTTCTTATAGCTTTATGTTTAATTTAACCGTGTCTTTAGATGGTTATGGTAATTTTGCTCGAATTAGCGATGCTATTGCAGCAGCACCAAACTTCAGCACTACTAGATTTTATATTCACGTGAAACCTGGTATTTATCACGAGTATGTTGAAGTTCCAATTCAAAAGACATTCATTGCTTTGATTGGAGATGATGCTTCTACAACCATCATCGTTGGTAATCGAAGTAATGACACTGGATTTTCAACTCCAACAACTGCAACCCTCAGTGTGAAAGGTGAAAACTTCATGGCTAAGTTGTTGACTTTTGAAAACTCTGCTGGACCAAAGAAAGGGCAAGCAGTGGCTGTTTTCAACATGGCGAACTACACATCGTACTACAAATGTGTATTTTTAGGTTATCAAGACACCCTTTATGCAGGAGCATTACCACAATTCTTCAAAGAGTGCGACATATACGGAACCGTAGACTTTATTTTCGGAAACGGACTTGCAGTATTCCAAGACTGTAATGTATACGCTCGATTACCCGACATTCAAATTACAATCACTGCACAATCCAAAGACAACATAAACAGTTTGAGTGGCTTCGTGTTCCAAAACTGCAATGTGACAGTGTCACCAGACATAACATCAAGCAAGGACAATGTTACTGTGTTCCTTGGACGACCATGGAGGAAATATTCGATGGTCGTTTTCATGGAGTCGTTTCTTGACAACGTAGTTCAACCAAGAGGATGGTTGGAGTGGCCAGGTGAGCCATTGAATTTACTATTTTATGCTGAATTTAATAATAATGGTCCAGGAGCTGACACTTCTCGAAGAGTCAATTGGCCTACTTACCATGTATTGCATAATACAATGGATGTTGCAAAGTTTACGGTTGGAAATTTCATTGATGGGACTAAATGGCTACCCCAAACTGGCATCCCTTTTAGGACTGGCTTGTGA

Coding sequence (CDS)

ATGGCATTTCAACTACATGTGTTCTTTATAGTTAGCTTACTAATTTTCATCAATCCTTTTGTTGAATTTTCTTATAGCTTTATGTTTAATTTAACCGTGTCTTTAGATGGTTATGGTAATTTTGCTCGAATTAGCGATGCTATTGCAGCAGCACCAAACTTCAGCACTACTAGATTTTATATTCACGTGAAACCTGGTATTTATCACGAGTATGTTGAAGTTCCAATTCAAAAGACATTCATTGCTTTGATTGGAGATGATGCTTCTACAACCATCATCGTTGGTAATCGAAGTAATGACACTGGATTTTCAACTCCAACAACTGCAACCCTCAGTGTGAAAGGTGAAAACTTCATGGCTAAGTTGTTGACTTTTGAAAACTCTGCTGGACCAAAGAAAGGGCAAGCAGTGGCTGTTTTCAACATGGCGAACTACACATCGTACTACAAATGTGTATTTTTAGGTTATCAAGACACCCTTTATGCAGGAGCATTACCACAATTCTTCAAAGAGTGCGACATATACGGAACCGTAGACTTTATTTTCGGAAACGGACTTGCAGTATTCCAAGACTGTAATGTATACGCTCGATTACCCGACATTCAAATTACAATCACTGCACAATCCAAAGACAACATAAACAGTTTGAGTGGCTTCGTGTTCCAAAACTGCAATGTGACAGTGTCACCAGACATAACATCAAGCAAGGACAATGTTACTGTGTTCCTTGGACGACCATGGAGGAAATATTCGATGGTCGTTTTCATGGAGTCGTTTCTTGACAACGTAGTTCAACCAAGAGGATGGTTGGAGTGGCCAGGTGAGCCATTGAATTTACTATTTTATGCTGAATTTAATAATAATGGTCCAGGAGCTGACACTTCTCGAAGAGTCAATTGGCCTACTTACCATGTATTGCATAATACAATGGATGTTGCAAAGTTTACGGTTGGAAATTTCATTGATGGGACTAAATGGCTACCCCAAACTGGCATCCCTTTTAGGACTGGCTTGTGA

Protein sequence

MAFQLHVFFIVSLLIFINPFVEFSYSFMFNLTVSLDGYGNFARISDAIAAAPNFSTTRFYIHVKPGIYHEYVEVPIQKTFIALIGDDASTTIIVGNRSNDTGFSTPTTATLSVKGENFMAKLLTFENSAGPKKGQAVAVFNMANYTSYYKCVFLGYQDTLYAGALPQFFKECDIYGTVDFIFGNGLAVFQDCNVYARLPDIQITITAQSKDNINSLSGFVFQNCNVTVSPDITSSKDNVTVFLGRPWRKYSMVVFMESFLDNVVQPRGWLEWPGEPLNLLFYAEFNNNGPGADTSRRVNWPTYHVLHNTMDVAKFTVGNFIDGTKWLPQTGIPFRTGL

Homology

[Close]

BLAST of Tan0006710 vs. ExPASy Swiss-Prot
Match: O81415 (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3702 GN=PME39 PE=2 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 6.8e-86
Identity = 151/312 (48.40%), Postives = 212/312 (67.95%), Query Frame = 0


Query: 29  FNLTVSLDGYGNFARISDAIAAAPNFSTTRFYIHVKPGIYHEYVEVPIQKTFIALIGDDA 88
           +NL+V++DG GNF  I+DA+ AAPN S TRF I++K G Y E VE+P +KT I  IGD  
Sbjct: 220 YNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDGI 279

Query: 89  STTIIVGNRSNDTGFSTPTTATLSVKGENFMAKLLTFENSAGPKKGQAVAVFNMANYTSY 148
             T+I  NRS   G+ST  T T+ VKG+ ++AK ++F NSAGP K QAVA  + ++++++
Sbjct: 280 GKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGSDHSAF 339

Query: 149 YKCVFLGYQDTLYAGALPQFFKECDIYGTVDFIFGNGLAVFQDCNVYARLPD--IQITIT 208
           Y+C F GYQDTLY  +  QF++ECDIYGT+DFIFGN   VFQ+ ++YAR P+   +I  T
Sbjct: 340 YRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAFT 399

Query: 209 AQSKDNINSLSGFVFQNCNVTVSPDITSSKDNVTVFLGRPWRKYSMVVFMESFLDNVVQP 268
           AQS++  +  +G    NC +  +PD+   K+N   +LGRPWRKYS  V ++SF+D+++ P
Sbjct: 400 AQSRNQSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIHP 459

Query: 269 RGWLEWPGE-PLNLLFYAEFNNNGPGADTSRRVNWPTYHVLHNTMDVAKFTVGNFIDGTK 328
            GWLE   +  L  L+Y E+ N GPGA+ ++RV WP +  + N  +  +FTVG FIDG+ 
Sbjct: 460 AGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGST 519

Query: 329 WLPQTGIPFRTG 338
           WL  TGIPF  G
Sbjct: 520 WLNSTGIPFSLG 531

[Close]

BLAST of Tan0006710 vs. ExPASy Swiss-Prot
Match: O48711 (Probable pectinesterase/pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PME12 PE=2 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 2.7e-82
Identity = 148/312 (47.44%), Postives = 208/312 (66.67%), Query Frame = 0


Query: 30  NLTVSLDGYGNFARISDAIAAAPNFSTTRFYIHVKPGIYHEYVEVPIQKTFIALIGDDAS 89
           +L V+ DG GNF+ I++AI+ APN S  R  I+VK G+Y E +++PI KT I LIGD + 
Sbjct: 237 SLVVAADGTGNFSTINEAISFAPNMSNDRVLIYVKEGVYDENIDIPIYKTNIVLIGDGSD 296

Query: 90  TTIIVGNRSNDTGFSTPTTATLSVKGENFMAKLLTFENSAGPKKGQAVAVFNMANYTSYY 149
            T I GNRS   G++T  +ATL+V GE F+A+ +   N+AGP+K QAVA+   A++ + Y
Sbjct: 297 VTFITGNRSVGDGWTTFRSATLAVSGEGFLARDIMITNTAGPEKHQAVALRVNADFVALY 356

Query: 150 KCVFLGYQDTLYAGALPQFFKECDIYGTVDFIFGNGLAVFQDCNVYARLP-DIQIT-ITA 209
           +CV  GYQDTLY  +  QF++ECDIYGT+D+IFGN   VFQ CN+ ++LP   Q T ITA
Sbjct: 357 RCVIDGYQDTLYTHSFRQFYRECDIYGTIDYIFGNAAVVFQGCNIVSKLPMPGQFTVITA 416

Query: 210 QSKDNINSLSGFVFQNCNVTVSPDITSSKDNVTVFLGRPWRKYSMVVFMESFLDNVVQPR 269
           QS+D  +  +G   QNC++  S D+ +S + V  +LGRPWR++S  V MES++D  +   
Sbjct: 417 QSRDTQDEDTGISMQNCSILASEDLFNSSNKVKSYLGRPWREFSRTVVMESYIDEFIDGS 476

Query: 270 GWLEW-PGEPLNLLFYAEFNNNGPGADTSRRVNWPTYHVLHNTMDVAKFTVGNFIDGTKW 329
           GW +W  GE L+ L+Y E+NNNGPG++T +RVNWP +H++    D   FT   FI G  W
Sbjct: 477 GWSKWNGGEALDTLYYGEYNNNGPGSETVKRVNWPGFHIM-GYEDAFNFTATEFITGDGW 536

Query: 330 LPQTGIPFRTGL 339
           L  T  P+  G+
Sbjct: 537 LGSTSFPYDNGI 547

[Close]

BLAST of Tan0006710 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 2.7e-82
Identity = 143/311 (45.98%), Postives = 210/311 (67.52%), Query Frame = 0


Query: 30  NLTVSLDGYGNFARISDAIAAAPNFSTTRFYIHVKPGIYHEYVEVPIQKTFIALIGDDAS 89
           N+ V+ DG GN   I +A+AAA     +R+ I++K G Y+E +EV ++   I  +GD   
Sbjct: 202 NIVVAQDGSGNVKTIQEAVAAASRAGGSRYVIYIKAGTYNENIEVKLKN--IMFVGDGIG 261

Query: 90  TTIIVGNRSNDTGFSTPTTATLSVKGENFMAKLLTFENSAGPKKGQAVAVFNMANYTSYY 149
            TII G++S   G +T  +AT++V G+NF+A+ +T  N+AGP   QAVA+ + ++ + +Y
Sbjct: 262 KTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIRNTAGPNNHQAVALRSGSDLSVFY 321

Query: 150 KCVFLGYQDTLYAGALPQFFKECDIYGTVDFIFGNGLAVFQDCNVYARLPDIQI-TITAQ 209
           +C F GYQDTLY  +  QF++ECDIYGTVDFIFGN   V Q+CN++AR P  +  T+TAQ
Sbjct: 322 RCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNCNIFARKPPNRTNTLTAQ 381

Query: 210 SKDNINSLSGFVFQNCNVTVSPDITSSKDNVTVFLGRPWRKYSMVVFMESFLDNVVQPRG 269
            + + N  +G +  NC VT + D+   + +V  FLGRPW++YS  V++++FLD+++ P G
Sbjct: 382 GRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWKQYSRTVYIKTFLDSLINPAG 441

Query: 270 WLEWPGE-PLNLLFYAEFNNNGPGADTSRRVNWPTYHVLHNTMDVAKFTVGNFIDGTKWL 329
           W+EW G+  LN L+YAE+ N GPG+ T+ RV W  YHVL +   V++FTVGNFI G  WL
Sbjct: 442 WMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNSWL 501

Query: 330 PQTGIPFRTGL 339
           P T +PF +GL
Sbjct: 502 PATNVPFTSGL 510

[Close]

BLAST of Tan0006710 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 2.3e-81
Identity = 147/314 (46.82%), Postives = 210/314 (66.88%), Query Frame = 0


Query: 31  LTVSLDGYGNFARISDAIAAAPN---FSTTRFYIHVKPGIYHEYVEVPIQKTFIALIGDD 90
           +TVS DG GNF  I+ A+AAAPN    S   F I+V  GIY EY+ +   K ++ +IGD 
Sbjct: 265 VTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEYISIAKNKRYMMMIGDG 324

Query: 91  ASTTIIVGNRSNDTGFSTPTTATLSVKGENFMAKLLTFENSAGPKKGQAVAVFNMANYTS 150
            + T++ GNRS   G++T  +AT +V   NF+A  +TF N+AGP+K QAVA+ + A+++ 
Sbjct: 325 INQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNTAGPEKHQAVALRSGADFSI 384

Query: 151 YYKCVFLGYQDTLYAGALPQFFKECDIYGTVDFIFGNGLAVFQDCNVYAR--LPDIQITI 210
           +Y C F  YQDTLY  +L QF++ECD+YGTVDFIFGN   VFQ+CN+Y R  +P+    I
Sbjct: 385 FYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAI 444

Query: 211 TAQSKDNINSLSGFVFQNCNVTVSPDITSSKDNVTVFLGRPWRKYSMVVFMESFLDNVVQ 270
           TAQ + + N  +G   QNC +  + D+ SS   V  +LGRPW++YS  V+M+S++D  V+
Sbjct: 445 TAQGRSDPNQNTGTSIQNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVYMQSYIDGFVE 504

Query: 271 PRGWLEWPGE-PLNLLFYAEFNNNGPGADTSRRVNWPTYHVLHNTMDVAKFTVGNFIDGT 330
           P GW EW G+  L+ L+YAE+NN GPG++T+ RV WP YHV+++T D A FTV       
Sbjct: 505 PVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVINST-DAANFTVTGLFIEA 564

Query: 331 KWLPQTGIPFRTGL 339
            W+ +TG+P+ +GL
Sbjct: 565 DWIWKTGVPYTSGL 577

[Close]

BLAST of Tan0006710 vs. ExPASy Swiss-Prot
Match: Q9LXK7 (Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana OX=3702 GN=PME32 PE=2 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 3.8e-81
Identity = 145/309 (46.93%), Postives = 202/309 (65.37%), Query Frame = 0


Query: 33  VSLDGYGNFARISDAIAAAPNFSTTRFYIHVKPGIYHEYVEVPIQKTFIALIGDDASTTI 92
           V+ DG GNF  ISDA+ AAP++ST R+ IHVK G+Y E VE+  +K  I ++GD    T+
Sbjct: 217 VAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVENVEIKKKKWNIMMVGDGIDATV 276

Query: 93  IVGNRSNDTGFSTPTTATLSVKGENFMAKLLTFENSAGPKKGQAVAVFNMANYTSYYKCV 152
           I GNRS   G++T  +AT +V G  F+A+ +TF+N+AGP+K QAVA+ +  +   +Y+C 
Sbjct: 277 ITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAGPEKHQAVAIRSDTDLGVFYRCA 336

Query: 153 FLGYQDTLYAGALPQFFKECDIYGTVDFIFGNGLAVFQDCNVYAR--LPDIQITITAQSK 212
             GYQDTLYA ++ QFF+EC I GTVDFIFG+  AVFQ C + A+  LP+ + +ITAQ +
Sbjct: 337 MRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQSCQIKAKQGLPNQKNSITAQGR 396

Query: 213 DNINSLSGFVFQNCNVTVSPDITSSKDNVTVFLGRPWRKYSMVVFMESFLDNVVQPRGWL 272
            + N  +GF  Q  N+    D+  + +    +LGRPW+ YS  VFM++++ + + P GWL
Sbjct: 397 KDPNEPTGFTIQFSNIAADTDLLLNLNTTATYLGRPWKLYSRTVFMQNYMSDAINPVGWL 456

Query: 273 EWPGE-PLNLLFYAEFNNNGPGADTSRRVNWPTYHVLHNTMDVAKFTVGNFIDGTKWLPQ 332
           EW G   L+ L+Y E+ N+GPGA   RRV WP YHVL+ + +   FTV   I G  WLP 
Sbjct: 457 EWNGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHVLNTSAEANNFTVSQLIQGNLWLPS 516

Query: 333 TGIPFRTGL 339
           TGI F  GL
Sbjct: 517 TGITFIAGL 525

[Close]

BLAST of Tan0006710 vs. NCBI nr
Match: XP_022150332.1 (probable pectinesterase/pectinesterase inhibitor 39 [Momordica charantia])

HSP 1 Score: 555.8 bits (1431), Expect = 2.5e-154
Identity = 262/338 (77.51%), Postives = 297/338 (87.87%), Query Frame = 0


Query: 1   MAFQLHVFFIVSLLIFINPFVEFSYSFMFNLTVSLDGYGNFARISDAIAAAPNFSTTRFY 60
           MAF+L  FF+VSLL+ IN FV+FSY FMFN TVS DG GNF R+SDAIAAAPNFSTTRFY
Sbjct: 1   MAFKLLGFFVVSLLVVINHFVKFSYCFMFNATVSPDGSGNFVRVSDAIAAAPNFSTTRFY 60

Query: 61  IHVKPGIYHEYVEVPIQKTFIALIGDDASTTIIVGNRSNDTGFSTPTTATLSVKGENFMA 120
           IHVKPGIY E+VEVP+QKTFIALIG+DASTTIIV NRSN +GFST TTATL VKG+NFMA
Sbjct: 61  IHVKPGIYKEHVEVPVQKTFIALIGEDASTTIIVDNRSNGSGFSTQTTATLIVKGQNFMA 120

Query: 121 KLLTFENSAGPKKGQAVAVFNMANYTSYYKCVFLGYQDTLYAGALPQFFKECDIYGTVDF 180
           + LTFENSA  K+GQA+AV + A +T+YYKCVFL YQDTLYAGA+PQFFKECDIYG +DF
Sbjct: 121 QFLTFENSAEQKEGQAIAVMDQAKHTAYYKCVFLSYQDTLYAGAVPQFFKECDIYGRIDF 180

Query: 181 IFGNGLAVFQDCNVYARLPDIQITITAQSKDNINSLSGFVFQNCNVTVSPDITSSKDNVT 240
           IFGNGL VFQDCN+YAR+P   I +TAQSK+ +   SGF+FQNCNVTVSP+I SSKDNVT
Sbjct: 181 IFGNGLVVFQDCNIYARIPGTHIIVTAQSKNAVEDQSGFIFQNCNVTVSPEIASSKDNVT 240

Query: 241 VFLGRPWRKYSMVVFMESFLDNVVQPRGWLEWPGEPLNLLFYAEFNNNGPGADTSRRVNW 300
           +FLGRPWR YSMV+FMESFLD VVQP+GWLEWPG P+NLLFYAE+NN GPGADTS+RV+W
Sbjct: 241 MFLGRPWRDYSMVIFMESFLDRVVQPKGWLEWPGRPVNLLFYAEYNNRGPGADTSQRVDW 300

Query: 301 PTYHVLHNTMDVAKFTVGNFIDGTKWLPQTGIPFRTGL 339
           P YHVLHN M+V KFTV NFI+GTKWLP+TGIPFR GL
Sbjct: 301 PGYHVLHNAMEVTKFTVENFINGTKWLPETGIPFRAGL 338

[Close]

BLAST of Tan0006710 vs. NCBI nr
Match: XP_022150325.1 (probable pectinesterase/pectinesterase inhibitor 44 [Momordica charantia])

HSP 1 Score: 540.4 bits (1391), Expect = 1.1e-149
Identity = 254/338 (75.15%), Postives = 299/338 (88.46%), Query Frame = 0


Query: 1   MAFQLHVFFIVSLLIFINPFVEFSYSFMFNLTVSLDGYGNFARISDAIAAAPNFSTTRFY 60
           M F++ +FFIV+LL+ ++ FVEFSYSF FN+TVSLDG GNF R++DAIAAAPNFST+RFY
Sbjct: 1   MTFKVVMFFIVTLLVILDHFVEFSYSFTFNITVSLDGMGNFFRVNDAIAAAPNFSTSRFY 60

Query: 61  IHVKPGIYHEYVEVPIQKTFIALIGDDASTTIIVGNRSNDTGFSTPTTATLSVKGENFMA 120
           IHVKPG Y+E+VEVP QKTFIALIGDDASTTIIV NRSN TGFST T+ATL+V GENFMA
Sbjct: 61  IHVKPGTYNEHVEVPAQKTFIALIGDDASTTIIVDNRSNGTGFSTATSATLTVSGENFMA 120

Query: 121 KLLTFENSAGPKKGQAVAVFNMANYTSYYKCVFLGYQDTLYAGALPQFFKECDIYGTVDF 180
           + LTF+NSAGP KGQAVAV + A +T YYKCVFLGYQDTL+AGA+PQFFKECDIYG+VDF
Sbjct: 121 QSLTFQNSAGPDKGQAVAVLDQAKHTVYYKCVFLGYQDTLFAGAVPQFFKECDIYGSVDF 180

Query: 181 IFGNGLAVFQDCNVYARLPDIQITITAQSKDNINSLSGFVFQNCNVTVSPDITSSKDNVT 240
           IFGNGLA+FQDCN+YARL DI IT+TAQSKDN+++ SGF+FQ CNVTVSP+I SSK+ VT
Sbjct: 181 IFGNGLAIFQDCNIYARLSDIHITVTAQSKDNVDNHSGFIFQACNVTVSPEIVSSKNKVT 240

Query: 241 VFLGRPWRKYSMVVFMESFLDNVVQPRGWLEWPGEPLNLLFYAEFNNNGPGADTSRRVNW 300
           VFLGRPWR YSMV F+ESFLD+VVQP+GWLEWPG P+NLLFYAE+ N+G G++TS RV+W
Sbjct: 241 VFLGRPWRTYSMVAFIESFLDDVVQPKGWLEWPGTPVNLLFYAEYKNSGTGSETSNRVDW 300

Query: 301 PTYHVLHNTMDVAKFTVGNFIDGTKWLPQTGIPFRTGL 339
           P +H+     +VA FTV NFIDGT+WLP+TGIPFRTGL
Sbjct: 301 PGFHLFSYAAEVANFTVENFIDGTQWLPETGIPFRTGL 338

[Close]

BLAST of Tan0006710 vs. NCBI nr
Match: XP_022151324.1 (pectinesterase 2-like [Momordica charantia])

HSP 1 Score: 533.9 bits (1374), Expect = 1.0e-147
Identity = 254/338 (75.15%), Postives = 292/338 (86.39%), Query Frame = 0


Query: 1   MAFQLHVFFIVSLLIFINPFVEFSYSFMFNLTVSLDGYGNFARISDAIAAAPNFSTTRFY 60
           MAF   V F+VSLL+ IN FV+FSYSF FN  VSLDG GNF R+SDA AAAPNF TTRFY
Sbjct: 1   MAFNFLVLFVVSLLVVINHFVKFSYSFRFNAVVSLDGTGNFVRVSDATAAAPNFGTTRFY 60

Query: 61  IHVKPGIYHEYVEVPIQKTFIALIGDDASTTIIVGNRSNDTGFSTPTTATLSVKGENFMA 120
           IH+KPGIY E+VEVP+QKTFIALIGDDASTTIIV NRSN +GFST  TATL+VKG+NFMA
Sbjct: 61  IHIKPGIYKEHVEVPVQKTFIALIGDDASTTIIVDNRSNGSGFSTSATATLTVKGQNFMA 120

Query: 121 KLLTFENSAGPKKGQAVAVFNMANYTSYYKCVFLGYQDTLYAGALPQFFKECDIYGTVDF 180
           + LTF+NSAGP + QA+A+ + A +T+YYKCVFLGYQDTLYA A+PQFFKECDIYG+VDF
Sbjct: 121 QSLTFQNSAGPNEHQAIALLDQAKHTAYYKCVFLGYQDTLYAAAVPQFFKECDIYGSVDF 180

Query: 181 IFGNGLAVFQDCNVYARLPDIQITITAQSKDNINSLSGFVFQNCNVTVSPDITSSKDNVT 240
           IFGNGLAVFQDCN+YA+L  I IT+TAQSKD +N  SGF+FQ CNVTVSP+I SS+D V 
Sbjct: 181 IFGNGLAVFQDCNIYAQLSGI-ITVTAQSKDKVNDHSGFIFQRCNVTVSPNIASSRDKVI 240

Query: 241 VFLGRPWRKYSMVVFMESFLDNVVQPRGWLEWPGEPLNLLFYAEFNNNGPGADTSRRVNW 300
           +FLGRPWR YSMVVF+ES+LDNVVQP+GWLEWPG P+NLLFYAE+ N+GPGA T +RV+W
Sbjct: 241 MFLGRPWRTYSMVVFIESYLDNVVQPKGWLEWPGVPVNLLFYAEYKNSGPGAHTPQRVHW 300

Query: 301 PTYHVLHNTMDVAKFTVGNFIDGTKWLPQTGIPFRTGL 339
           P YHVL+N   VAKFTV NFID TKWLP+TGIPFRTGL
Sbjct: 301 PGYHVLYNAKQVAKFTVENFIDWTKWLPETGIPFRTGL 337

[Close]

BLAST of Tan0006710 vs. NCBI nr
Match: XP_011654361.2 (probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus])

HSP 1 Score: 514.2 bits (1323), Expect = 8.4e-142
Identity = 245/335 (73.13%), Postives = 287/335 (85.67%), Query Frame = 0


Query: 3   FQLHVFFIVSLLIFINPFVEFSYSFMFNLTVSLDGYGNFARISDAIAAAPNFSTTRFYIH 62
           F L + F++ LL+ +   VEFS  F FN TVSLDG  NF +I+DAIAAAPNFSTTRFYIH
Sbjct: 4   FTLEMIFVLYLLVVV--IVEFSDCFSFNSTVSLDGTENFIKINDAIAAAPNFSTTRFYIH 63

Query: 63  VKPGIYHEYVEVPIQKTFIALIGDDASTTIIVGNRSNDTGFSTPTTATLSVKGENFMAKL 122
           VKPG Y E +EVP +KT IALIGDD+STTIIV NRSN TG ST ++ATL+V G NFMA+ 
Sbjct: 64  VKPGTYQEIIEVPYEKTCIALIGDDSSTTIIVNNRSNGTGSSTASSATLTVNGSNFMAQF 123

Query: 123 LTFENSAGPKKGQAVAVFNMANYTSYYKCVFLGYQDTLYAGALPQFFKECDIYGTVDFIF 182
           LTF+N+AGP +GQA+A+ + A +T+YYKCVFLGYQDTLYAGALPQFFKECDIYG+VDFIF
Sbjct: 124 LTFQNTAGPNEGQAIALLDQATHTAYYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIF 183

Query: 183 GNGLAVFQDCNVYARLPDIQITITAQSKDNINSLSGFVFQNCNVTVSPDITSSKDNVTVF 242
           GNGL VFQDCN+YARL D+QIT+TAQSK ++NSLSGF+FQNCNVTVSP+I  SKDNVTVF
Sbjct: 184 GNGLVVFQDCNIYARLFDVQITVTAQSKPSLNSLSGFIFQNCNVTVSPEIAPSKDNVTVF 243

Query: 243 LGRPWRKYSMVVFMESFLDNVVQPRGWLEWPGEPLNLLFYAEFNNNGPGADTSRRVNWPT 302
           LGRPWR YSMVVF++SFLDNVVQP+GWLEWPG P NLL+YAE+NN+G GA+TS+RVNWP 
Sbjct: 244 LGRPWRMYSMVVFIDSFLDNVVQPKGWLEWPGVPENLLYYAEYNNSGGGANTSQRVNWPG 303

Query: 303 YHVLHNTMDVAKFTVGNFIDGTKWLPQTGIPFRTG 338
           YH L+N  +VA FTV  FI+GT+WLPQTGIPFR G
Sbjct: 304 YHALNNAKEVATFTVETFINGTQWLPQTGIPFRAG 336

[Close]

BLAST of Tan0006710 vs. NCBI nr
Match: XP_023002625.1 (probable pectinesterase/pectinesterase inhibitor 39 [Cucurbita maxima])

HSP 1 Score: 513.8 bits (1322), Expect = 1.1e-141
Identity = 247/338 (73.08%), Postives = 288/338 (85.21%), Query Frame = 0


Query: 1   MAFQLHVFFIVSLLIFINPFVEFSYSFMFNLTVSLDGYGNFARISDAIAAAPNFSTTRFY 60
           MAFQ  +FF +S+L+    F++FS +F FN TVSLDG GNF +ISDAI AAPNF+TTRFY
Sbjct: 1   MAFQHVMFFTLSILLL--HFLKFSSTFDFNSTVSLDGTGNFVKISDAIVAAPNFTTTRFY 60

Query: 61  IHVKPGIYHEYVEVPIQKTFIALIGDDASTTIIVGNRSNDTGFSTPTTATLSVKGENFMA 120
           IH+KPGIY+E++E+P QKTFI+LIGD+ASTTIIV NRS  TGFST  +ATL+V G NFMA
Sbjct: 61  IHIKPGIYNEHIEIPSQKTFISLIGDNASTTIIVDNRSKATGFSTANSATLTVNGANFMA 120

Query: 121 KLLTFENSAGPKKGQAVAVFNMANYTSYYKCVFLGYQDTLYAGALPQFFKECDIYGTVDF 180
           K LTF+NSAGPK GQAVAV + A +T+YYKCVFLGYQDTLYA A+PQFFKECDIYGTVDF
Sbjct: 121 KFLTFQNSAGPKMGQAVAVLDQAKHTAYYKCVFLGYQDTLYAAAVPQFFKECDIYGTVDF 180

Query: 181 IFGNGLAVFQDCNVYARLPDIQITITAQSKDNINSLSGFVFQNCNVTVSPDITSSKDNVT 240
           IFGNGL VFQDCN+YARL DIQ T+TAQSK N++  SGFVFQNC VTVSP+I SS+D V 
Sbjct: 181 IFGNGLVVFQDCNIYARLVDIQSTVTAQSKANLDEQSGFVFQNCTVTVSPEIASSRDKVL 240

Query: 241 VFLGRPWRKYSMVVFMESFLDNVVQPRGWLEWPGEPLNLLFYAEFNNNGPGADTSRRVNW 300
           VFLGRPWR YSMV F+ESFLDNVVQP+GWLEW G P+NLL+Y E+NN G GA+TS+RV+W
Sbjct: 241 VFLGRPWRAYSMVAFVESFLDNVVQPKGWLEWLGVPVNLLYYGEYNNRGGGANTSQRVDW 300

Query: 301 PTYHVLHNTMDVAKFTVGNFIDGTKWLPQTGIPFRTGL 339
           P +HVLH+  DVAKFTV NFI+GT WLPQT IPFR+GL
Sbjct: 301 PGFHVLHSLTDVAKFTVENFINGTTWLPQTTIPFRSGL 336

[Close]

BLAST of Tan0006710 vs. ExPASy TrEMBL
Match: A0A6J1DAG5 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018528 PE=4 SV=1)

HSP 1 Score: 555.8 bits (1431), Expect = 1.2e-154
Identity = 262/338 (77.51%), Postives = 297/338 (87.87%), Query Frame = 0


Query: 1   MAFQLHVFFIVSLLIFINPFVEFSYSFMFNLTVSLDGYGNFARISDAIAAAPNFSTTRFY 60
           MAF+L  FF+VSLL+ IN FV+FSY FMFN TVS DG GNF R+SDAIAAAPNFSTTRFY
Sbjct: 1   MAFKLLGFFVVSLLVVINHFVKFSYCFMFNATVSPDGSGNFVRVSDAIAAAPNFSTTRFY 60

Query: 61  IHVKPGIYHEYVEVPIQKTFIALIGDDASTTIIVGNRSNDTGFSTPTTATLSVKGENFMA 120
           IHVKPGIY E+VEVP+QKTFIALIG+DASTTIIV NRSN +GFST TTATL VKG+NFMA
Sbjct: 61  IHVKPGIYKEHVEVPVQKTFIALIGEDASTTIIVDNRSNGSGFSTQTTATLIVKGQNFMA 120

Query: 121 KLLTFENSAGPKKGQAVAVFNMANYTSYYKCVFLGYQDTLYAGALPQFFKECDIYGTVDF 180
           + LTFENSA  K+GQA+AV + A +T+YYKCVFL YQDTLYAGA+PQFFKECDIYG +DF
Sbjct: 121 QFLTFENSAEQKEGQAIAVMDQAKHTAYYKCVFLSYQDTLYAGAVPQFFKECDIYGRIDF 180

Query: 181 IFGNGLAVFQDCNVYARLPDIQITITAQSKDNINSLSGFVFQNCNVTVSPDITSSKDNVT 240
           IFGNGL VFQDCN+YAR+P   I +TAQSK+ +   SGF+FQNCNVTVSP+I SSKDNVT
Sbjct: 181 IFGNGLVVFQDCNIYARIPGTHIIVTAQSKNAVEDQSGFIFQNCNVTVSPEIASSKDNVT 240

Query: 241 VFLGRPWRKYSMVVFMESFLDNVVQPRGWLEWPGEPLNLLFYAEFNNNGPGADTSRRVNW 300
           +FLGRPWR YSMV+FMESFLD VVQP+GWLEWPG P+NLLFYAE+NN GPGADTS+RV+W
Sbjct: 241 MFLGRPWRDYSMVIFMESFLDRVVQPKGWLEWPGRPVNLLFYAEYNNRGPGADTSQRVDW 300

Query: 301 PTYHVLHNTMDVAKFTVGNFIDGTKWLPQTGIPFRTGL 339
           P YHVLHN M+V KFTV NFI+GTKWLP+TGIPFR GL
Sbjct: 301 PGYHVLHNAMEVTKFTVENFINGTKWLPETGIPFRAGL 338

[Close]

BLAST of Tan0006710 vs. ExPASy TrEMBL
Match: A0A6J1D863 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018522 PE=4 SV=1)

HSP 1 Score: 540.4 bits (1391), Expect = 5.3e-150
Identity = 254/338 (75.15%), Postives = 299/338 (88.46%), Query Frame = 0


Query: 1   MAFQLHVFFIVSLLIFINPFVEFSYSFMFNLTVSLDGYGNFARISDAIAAAPNFSTTRFY 60
           M F++ +FFIV+LL+ ++ FVEFSYSF FN+TVSLDG GNF R++DAIAAAPNFST+RFY
Sbjct: 1   MTFKVVMFFIVTLLVILDHFVEFSYSFTFNITVSLDGMGNFFRVNDAIAAAPNFSTSRFY 60

Query: 61  IHVKPGIYHEYVEVPIQKTFIALIGDDASTTIIVGNRSNDTGFSTPTTATLSVKGENFMA 120
           IHVKPG Y+E+VEVP QKTFIALIGDDASTTIIV NRSN TGFST T+ATL+V GENFMA
Sbjct: 61  IHVKPGTYNEHVEVPAQKTFIALIGDDASTTIIVDNRSNGTGFSTATSATLTVSGENFMA 120

Query: 121 KLLTFENSAGPKKGQAVAVFNMANYTSYYKCVFLGYQDTLYAGALPQFFKECDIYGTVDF 180
           + LTF+NSAGP KGQAVAV + A +T YYKCVFLGYQDTL+AGA+PQFFKECDIYG+VDF
Sbjct: 121 QSLTFQNSAGPDKGQAVAVLDQAKHTVYYKCVFLGYQDTLFAGAVPQFFKECDIYGSVDF 180

Query: 181 IFGNGLAVFQDCNVYARLPDIQITITAQSKDNINSLSGFVFQNCNVTVSPDITSSKDNVT 240
           IFGNGLA+FQDCN+YARL DI IT+TAQSKDN+++ SGF+FQ CNVTVSP+I SSK+ VT
Sbjct: 181 IFGNGLAIFQDCNIYARLSDIHITVTAQSKDNVDNHSGFIFQACNVTVSPEIVSSKNKVT 240

Query: 241 VFLGRPWRKYSMVVFMESFLDNVVQPRGWLEWPGEPLNLLFYAEFNNNGPGADTSRRVNW 300
           VFLGRPWR YSMV F+ESFLD+VVQP+GWLEWPG P+NLLFYAE+ N+G G++TS RV+W
Sbjct: 241 VFLGRPWRTYSMVAFIESFLDDVVQPKGWLEWPGTPVNLLFYAEYKNSGTGSETSNRVDW 300

Query: 301 PTYHVLHNTMDVAKFTVGNFIDGTKWLPQTGIPFRTGL 339
           P +H+     +VA FTV NFIDGT+WLP+TGIPFRTGL
Sbjct: 301 PGFHLFSYAAEVANFTVENFIDGTQWLPETGIPFRTGL 338

[Close]

BLAST of Tan0006710 vs. ExPASy TrEMBL
Match: A0A6J1DCN7 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111019286 PE=4 SV=1)

HSP 1 Score: 533.9 bits (1374), Expect = 4.9e-148
Identity = 254/338 (75.15%), Postives = 292/338 (86.39%), Query Frame = 0


Query: 1   MAFQLHVFFIVSLLIFINPFVEFSYSFMFNLTVSLDGYGNFARISDAIAAAPNFSTTRFY 60
           MAF   V F+VSLL+ IN FV+FSYSF FN  VSLDG GNF R+SDA AAAPNF TTRFY
Sbjct: 1   MAFNFLVLFVVSLLVVINHFVKFSYSFRFNAVVSLDGTGNFVRVSDATAAAPNFGTTRFY 60

Query: 61  IHVKPGIYHEYVEVPIQKTFIALIGDDASTTIIVGNRSNDTGFSTPTTATLSVKGENFMA 120
           IH+KPGIY E+VEVP+QKTFIALIGDDASTTIIV NRSN +GFST  TATL+VKG+NFMA
Sbjct: 61  IHIKPGIYKEHVEVPVQKTFIALIGDDASTTIIVDNRSNGSGFSTSATATLTVKGQNFMA 120

Query: 121 KLLTFENSAGPKKGQAVAVFNMANYTSYYKCVFLGYQDTLYAGALPQFFKECDIYGTVDF 180
           + LTF+NSAGP + QA+A+ + A +T+YYKCVFLGYQDTLYA A+PQFFKECDIYG+VDF
Sbjct: 121 QSLTFQNSAGPNEHQAIALLDQAKHTAYYKCVFLGYQDTLYAAAVPQFFKECDIYGSVDF 180

Query: 181 IFGNGLAVFQDCNVYARLPDIQITITAQSKDNINSLSGFVFQNCNVTVSPDITSSKDNVT 240
           IFGNGLAVFQDCN+YA+L  I IT+TAQSKD +N  SGF+FQ CNVTVSP+I SS+D V 
Sbjct: 181 IFGNGLAVFQDCNIYAQLSGI-ITVTAQSKDKVNDHSGFIFQRCNVTVSPNIASSRDKVI 240

Query: 241 VFLGRPWRKYSMVVFMESFLDNVVQPRGWLEWPGEPLNLLFYAEFNNNGPGADTSRRVNW 300
           +FLGRPWR YSMVVF+ES+LDNVVQP+GWLEWPG P+NLLFYAE+ N+GPGA T +RV+W
Sbjct: 241 MFLGRPWRTYSMVVFIESYLDNVVQPKGWLEWPGVPVNLLFYAEYKNSGPGAHTPQRVHW 300

Query: 301 PTYHVLHNTMDVAKFTVGNFIDGTKWLPQTGIPFRTGL 339
           P YHVL+N   VAKFTV NFID TKWLP+TGIPFRTGL
Sbjct: 301 PGYHVLYNAKQVAKFTVENFIDWTKWLPETGIPFRTGL 337

[Close]

BLAST of Tan0006710 vs. ExPASy TrEMBL
Match: A0A6J1KPG6 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111496427 PE=4 SV=1)

HSP 1 Score: 513.8 bits (1322), Expect = 5.3e-142
Identity = 247/338 (73.08%), Postives = 288/338 (85.21%), Query Frame = 0


Query: 1   MAFQLHVFFIVSLLIFINPFVEFSYSFMFNLTVSLDGYGNFARISDAIAAAPNFSTTRFY 60
           MAFQ  +FF +S+L+    F++FS +F FN TVSLDG GNF +ISDAI AAPNF+TTRFY
Sbjct: 1   MAFQHVMFFTLSILLL--HFLKFSSTFDFNSTVSLDGTGNFVKISDAIVAAPNFTTTRFY 60

Query: 61  IHVKPGIYHEYVEVPIQKTFIALIGDDASTTIIVGNRSNDTGFSTPTTATLSVKGENFMA 120
           IH+KPGIY+E++E+P QKTFI+LIGD+ASTTIIV NRS  TGFST  +ATL+V G NFMA
Sbjct: 61  IHIKPGIYNEHIEIPSQKTFISLIGDNASTTIIVDNRSKATGFSTANSATLTVNGANFMA 120

Query: 121 KLLTFENSAGPKKGQAVAVFNMANYTSYYKCVFLGYQDTLYAGALPQFFKECDIYGTVDF 180
           K LTF+NSAGPK GQAVAV + A +T+YYKCVFLGYQDTLYA A+PQFFKECDIYGTVDF
Sbjct: 121 KFLTFQNSAGPKMGQAVAVLDQAKHTAYYKCVFLGYQDTLYAAAVPQFFKECDIYGTVDF 180

Query: 181 IFGNGLAVFQDCNVYARLPDIQITITAQSKDNINSLSGFVFQNCNVTVSPDITSSKDNVT 240
           IFGNGL VFQDCN+YARL DIQ T+TAQSK N++  SGFVFQNC VTVSP+I SS+D V 
Sbjct: 181 IFGNGLVVFQDCNIYARLVDIQSTVTAQSKANLDEQSGFVFQNCTVTVSPEIASSRDKVL 240

Query: 241 VFLGRPWRKYSMVVFMESFLDNVVQPRGWLEWPGEPLNLLFYAEFNNNGPGADTSRRVNW 300
           VFLGRPWR YSMV F+ESFLDNVVQP+GWLEW G P+NLL+Y E+NN G GA+TS+RV+W
Sbjct: 241 VFLGRPWRAYSMVAFVESFLDNVVQPKGWLEWLGVPVNLLYYGEYNNRGGGANTSQRVDW 300

Query: 301 PTYHVLHNTMDVAKFTVGNFIDGTKWLPQTGIPFRTGL 339
           P +HVLH+  DVAKFTV NFI+GT WLPQT IPFR+GL
Sbjct: 301 PGFHVLHSLTDVAKFTVENFINGTTWLPQTTIPFRSGL 336

[Close]

BLAST of Tan0006710 vs. ExPASy TrEMBL
Match: A0A6J1GIG0 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111454459 PE=4 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 2.9e-140
Identity = 244/338 (72.19%), Postives = 284/338 (84.02%), Query Frame = 0


Query: 1   MAFQLHVFFIVSLLIFINPFVEFSYSFMFNLTVSLDGYGNFARISDAIAAAPNFSTTRFY 60
           MAFQ  +FF +S+L+    F++FS +F FN TVSLDG GNF +ISDAI AAPNF+TTRFY
Sbjct: 1   MAFQHVMFFTLSILLL--HFLKFSNTFDFNSTVSLDGTGNFVKISDAIVAAPNFTTTRFY 60

Query: 61  IHVKPGIYHEYVEVPIQKTFIALIGDDASTTIIVGNRSNDTGFSTPTTATLSVKGENFMA 120
           IH+KPGIY+E++EVP QKTFI+LIGD+ASTTIIV NRS   GFST  +ATL+V G NFMA
Sbjct: 61  IHIKPGIYNEHIEVPFQKTFISLIGDNASTTIIVDNRSKAAGFSTSNSATLTVNGANFMA 120

Query: 121 KLLTFENSAGPKKGQAVAVFNMANYTSYYKCVFLGYQDTLYAGALPQFFKECDIYGTVDF 180
           K LTF+NSAGPK GQAVAV + A +T+YYKCVFLGYQDTLYA A+PQFFKECDIYGTVDF
Sbjct: 121 KFLTFQNSAGPKMGQAVAVLDQAKHTAYYKCVFLGYQDTLYAAAVPQFFKECDIYGTVDF 180

Query: 181 IFGNGLAVFQDCNVYARLPDIQITITAQSKDNINSLSGFVFQNCNVTVSPDITSSKDNVT 240
           IFGNGL VFQDCN+YARL DI  T+TAQSK N++  SGFVFQNC VTVSP+I SS+D V 
Sbjct: 181 IFGNGLVVFQDCNIYARLVDIHTTVTAQSKANLDEQSGFVFQNCTVTVSPEIASSRDKVL 240

Query: 241 VFLGRPWRKYSMVVFMESFLDNVVQPRGWLEWPGEPLNLLFYAEFNNNGPGADTSRRVNW 300
           VFLGRPWR YSMV F+ESFLDNVVQP+GWLEW G P+NLL+Y E+NN G GA+TS+RV+W
Sbjct: 241 VFLGRPWRAYSMVAFVESFLDNVVQPKGWLEWLGVPVNLLYYGEYNNRGGGANTSQRVDW 300

Query: 301 PTYHVLHNTMDVAKFTVGNFIDGTKWLPQTGIPFRTGL 339
           P +HVLH+  DVA FTV NFI+GT WLP+T IPFR GL
Sbjct: 301 PGFHVLHSLTDVANFTVENFINGTTWLPETTIPFRAGL 336

[Close]

BLAST of Tan0006710 vs. TAIR 10
Match: AT4G02300.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 318.9 bits (816), Expect = 4.8e-87
Identity = 151/312 (48.40%), Postives = 212/312 (67.95%), Query Frame = 0


Query: 29  FNLTVSLDGYGNFARISDAIAAAPNFSTTRFYIHVKPGIYHEYVEVPIQKTFIALIGDDA 88
           +NL+V++DG GNF  I+DA+ AAPN S TRF I++K G Y E VE+P +KT I  IGD  
Sbjct: 220 YNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDGI 279

Query: 89  STTIIVGNRSNDTGFSTPTTATLSVKGENFMAKLLTFENSAGPKKGQAVAVFNMANYTSY 148
             T+I  NRS   G+ST  T T+ VKG+ ++AK ++F NSAGP K QAVA  + ++++++
Sbjct: 280 GKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGSDHSAF 339

Query: 149 YKCVFLGYQDTLYAGALPQFFKECDIYGTVDFIFGNGLAVFQDCNVYARLPD--IQITIT 208
           Y+C F GYQDTLY  +  QF++ECDIYGT+DFIFGN   VFQ+ ++YAR P+   +I  T
Sbjct: 340 YRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAFT 399

Query: 209 AQSKDNINSLSGFVFQNCNVTVSPDITSSKDNVTVFLGRPWRKYSMVVFMESFLDNVVQP 268
           AQS++  +  +G    NC +  +PD+   K+N   +LGRPWRKYS  V ++SF+D+++ P
Sbjct: 400 AQSRNQSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIHP 459

Query: 269 RGWLEWPGE-PLNLLFYAEFNNNGPGADTSRRVNWPTYHVLHNTMDVAKFTVGNFIDGTK 328
            GWLE   +  L  L+Y E+ N GPGA+ ++RV WP +  + N  +  +FTVG FIDG+ 
Sbjct: 460 AGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGST 519

Query: 329 WLPQTGIPFRTG 338
           WL  TGIPF  G
Sbjct: 520 WLNSTGIPFSLG 531

[Close]

BLAST of Tan0006710 vs. TAIR 10
Match: AT2G26440.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 307.0 bits (785), Expect = 1.9e-83
Identity = 148/312 (47.44%), Postives = 208/312 (66.67%), Query Frame = 0


Query: 30  NLTVSLDGYGNFARISDAIAAAPNFSTTRFYIHVKPGIYHEYVEVPIQKTFIALIGDDAS 89
           +L V+ DG GNF+ I++AI+ APN S  R  I+VK G+Y E +++PI KT I LIGD + 
Sbjct: 237 SLVVAADGTGNFSTINEAISFAPNMSNDRVLIYVKEGVYDENIDIPIYKTNIVLIGDGSD 296

Query: 90  TTIIVGNRSNDTGFSTPTTATLSVKGENFMAKLLTFENSAGPKKGQAVAVFNMANYTSYY 149
            T I GNRS   G++T  +ATL+V GE F+A+ +   N+AGP+K QAVA+   A++ + Y
Sbjct: 297 VTFITGNRSVGDGWTTFRSATLAVSGEGFLARDIMITNTAGPEKHQAVALRVNADFVALY 356

Query: 150 KCVFLGYQDTLYAGALPQFFKECDIYGTVDFIFGNGLAVFQDCNVYARLP-DIQIT-ITA 209
           +CV  GYQDTLY  +  QF++ECDIYGT+D+IFGN   VFQ CN+ ++LP   Q T ITA
Sbjct: 357 RCVIDGYQDTLYTHSFRQFYRECDIYGTIDYIFGNAAVVFQGCNIVSKLPMPGQFTVITA 416

Query: 210 QSKDNINSLSGFVFQNCNVTVSPDITSSKDNVTVFLGRPWRKYSMVVFMESFLDNVVQPR 269
           QS+D  +  +G   QNC++  S D+ +S + V  +LGRPWR++S  V MES++D  +   
Sbjct: 417 QSRDTQDEDTGISMQNCSILASEDLFNSSNKVKSYLGRPWREFSRTVVMESYIDEFIDGS 476

Query: 270 GWLEW-PGEPLNLLFYAEFNNNGPGADTSRRVNWPTYHVLHNTMDVAKFTVGNFIDGTKW 329
           GW +W  GE L+ L+Y E+NNNGPG++T +RVNWP +H++    D   FT   FI G  W
Sbjct: 477 GWSKWNGGEALDTLYYGEYNNNGPGSETVKRVNWPGFHIM-GYEDAFNFTATEFITGDGW 536

Query: 330 LPQTGIPFRTGL 339
           L  T  P+  G+
Sbjct: 537 LGSTSFPYDNGI 547

[Close]

BLAST of Tan0006710 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 303.9 bits (777), Expect = 1.6e-82
Identity = 147/314 (46.82%), Postives = 210/314 (66.88%), Query Frame = 0


Query: 31  LTVSLDGYGNFARISDAIAAAPN---FSTTRFYIHVKPGIYHEYVEVPIQKTFIALIGDD 90
           +TVS DG GNF  I+ A+AAAPN    S   F I+V  GIY EY+ +   K ++ +IGD 
Sbjct: 265 VTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEYISIAKNKRYMMMIGDG 324

Query: 91  ASTTIIVGNRSNDTGFSTPTTATLSVKGENFMAKLLTFENSAGPKKGQAVAVFNMANYTS 150
            + T++ GNRS   G++T  +AT +V   NF+A  +TF N+AGP+K QAVA+ + A+++ 
Sbjct: 325 INQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNTAGPEKHQAVALRSGADFSI 384

Query: 151 YYKCVFLGYQDTLYAGALPQFFKECDIYGTVDFIFGNGLAVFQDCNVYAR--LPDIQITI 210
           +Y C F  YQDTLY  +L QF++ECD+YGTVDFIFGN   VFQ+CN+Y R  +P+    I
Sbjct: 385 FYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAI 444

Query: 211 TAQSKDNINSLSGFVFQNCNVTVSPDITSSKDNVTVFLGRPWRKYSMVVFMESFLDNVVQ 270
           TAQ + + N  +G   QNC +  + D+ SS   V  +LGRPW++YS  V+M+S++D  V+
Sbjct: 445 TAQGRSDPNQNTGTSIQNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVYMQSYIDGFVE 504

Query: 271 PRGWLEWPGE-PLNLLFYAEFNNNGPGADTSRRVNWPTYHVLHNTMDVAKFTVGNFIDGT 330
           P GW EW G+  L+ L+YAE+NN GPG++T+ RV WP YHV+++T D A FTV       
Sbjct: 505 PVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVINST-DAANFTVTGLFIEA 564

Query: 331 KWLPQTGIPFRTGL 339
            W+ +TG+P+ +GL
Sbjct: 565 DWIWKTGVPYTSGL 577

[Close]

BLAST of Tan0006710 vs. TAIR 10
Match: AT3G43270.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 303.1 bits (775), Expect = 2.7e-82
Identity = 145/309 (46.93%), Postives = 202/309 (65.37%), Query Frame = 0


Query: 33  VSLDGYGNFARISDAIAAAPNFSTTRFYIHVKPGIYHEYVEVPIQKTFIALIGDDASTTI 92
           V+ DG GNF  ISDA+ AAP++ST R+ IHVK G+Y E VE+  +K  I ++GD    T+
Sbjct: 217 VAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVENVEIKKKKWNIMMVGDGIDATV 276

Query: 93  IVGNRSNDTGFSTPTTATLSVKGENFMAKLLTFENSAGPKKGQAVAVFNMANYTSYYKCV 152
           I GNRS   G++T  +AT +V G  F+A+ +TF+N+AGP+K QAVA+ +  +   +Y+C 
Sbjct: 277 ITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAGPEKHQAVAIRSDTDLGVFYRCA 336

Query: 153 FLGYQDTLYAGALPQFFKECDIYGTVDFIFGNGLAVFQDCNVYAR--LPDIQITITAQSK 212
             GYQDTLYA ++ QFF+EC I GTVDFIFG+  AVFQ C + A+  LP+ + +ITAQ +
Sbjct: 337 MRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQSCQIKAKQGLPNQKNSITAQGR 396

Query: 213 DNINSLSGFVFQNCNVTVSPDITSSKDNVTVFLGRPWRKYSMVVFMESFLDNVVQPRGWL 272
            + N  +GF  Q  N+    D+  + +    +LGRPW+ YS  VFM++++ + + P GWL
Sbjct: 397 KDPNEPTGFTIQFSNIAADTDLLLNLNTTATYLGRPWKLYSRTVFMQNYMSDAINPVGWL 456

Query: 273 EWPGE-PLNLLFYAEFNNNGPGADTSRRVNWPTYHVLHNTMDVAKFTVGNFIDGTKWLPQ 332
           EW G   L+ L+Y E+ N+GPGA   RRV WP YHVL+ + +   FTV   I G  WLP 
Sbjct: 457 EWNGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHVLNTSAEANNFTVSQLIQGNLWLPS 516

Query: 333 TGIPFRTGL 339
           TGI F  GL
Sbjct: 517 TGITFIAGL 525

[Close]

BLAST of Tan0006710 vs. TAIR 10
Match: AT4G33220.1 (pectin methylesterase 44 )

HSP 1 Score: 303.1 bits (775), Expect = 2.7e-82
Identity = 144/313 (46.01%), Postives = 208/313 (66.45%), Query Frame = 0


Query: 29  FNLTVSLDGYGNFARISDAIAAAPNFSTTRFYIHVKPGIYHEYVEVPIQKTFIALIGDDA 88
           ++++V+LDG GNF +I DAI  AP++S+TRF I++K G+Y E VE+  +K  I ++GD  
Sbjct: 211 YDVSVALDGTGNFTKIMDAIKKAPDYSSTRFVIYIKKGLYLENVEIKKKKWNIVMLGDGI 270

Query: 89  STTIIVGNRSNDTGFSTPTTATLSVKGENFMAKLLTFENSAGPKKGQAVAVFNMANYTSY 148
             T+I GNRS   G++T  +AT +V G  F+A+ +TF+N+AGP+K QAVA+ + ++ + +
Sbjct: 271 DVTVISGNRSFIDGWTTFRSATFAVSGRGFLARDITFQNTAGPEKHQAVALRSDSDLSVF 330

Query: 149 YKCVFLGYQDTLYAGALPQFFKECDIYGTVDFIFGNGLAVFQDCNVYAR--LPDIQITIT 208
           ++C   GYQDTLY   + QF++EC I GTVDFIFG+G  VFQ+C + A+  LP+ + TIT
Sbjct: 331 FRCAMRGYQDTLYTHTMRQFYRECTITGTVDFIFGDGTVVFQNCQILAKRGLPNQKNTIT 390

Query: 209 AQSKDNINSLSGFVFQNCNVTVSPDITSSKDNVTVFLGRPWRKYSMVVFMESFLDNVVQP 268
           AQ + ++N  SGF  Q  N++   D+    +    +LGRPW+ YS  VF+ + + +VV+P
Sbjct: 391 AQGRKDVNQPSGFSIQFSNISADADLVPYLNTTRTYLGRPWKLYSRTVFIRNNMSDVVRP 450

Query: 269 RGWLEWPGE-PLNLLFYAEFNNNGPGADTSRRVNWPTYHVLHNTMDVAKFTVGNFIDGTK 328
            GWLEW  +  L+ LFY EF N GPG+  S RV WP YHV +N+     FTV  FI G  
Sbjct: 451 EGWLEWNADFALDTLFYGEFMNYGPGSGLSSRVKWPGYHVFNNSDQANNFTVSQFIKGNL 510

Query: 329 WLPQTGIPFRTGL 339
           WLP TG+ F  GL
Sbjct: 511 WLPSTGVTFSDGL 523

The following BLAST results are available for this feature:

BLAST of Tan0006710 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81415	6.8e-86	48.40	Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3...	[more]
O48711	2.7e-82	47.44	Probable pectinesterase/pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3...	[more]
O04887	2.7e-82	45.98	Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1	[more]
Q9SRX4	2.3e-81	46.82	Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=37...	[more]
Q9LXK7	3.8e-81	46.93	Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of Tan0006710 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022150332.1	2.5e-154	77.51	probable pectinesterase/pectinesterase inhibitor 39 [Momordica charantia]	[more]
XP_022150325.1	1.1e-149	75.15	probable pectinesterase/pectinesterase inhibitor 44 [Momordica charantia]	[more]
XP_022151324.1	1.0e-147	75.15	pectinesterase 2-like [Momordica charantia]	[more]
XP_011654361.2	8.4e-142	73.13	probable pectinesterase/pectinesterase inhibitor 39 [Cucumis sativus]	[more]
XP_023002625.1	1.1e-141	73.08	probable pectinesterase/pectinesterase inhibitor 39 [Cucurbita maxima]	[more]

BLAST of Tan0006710 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DAG5	1.2e-154	77.51	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018528 PE=4 SV=1	[more]
A0A6J1D863	5.3e-150	75.15	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018522 PE=4 SV=1	[more]
A0A6J1DCN7	4.9e-148	75.15	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111019286 PE=4 SV=1	[more]
A0A6J1KPG6	5.3e-142	73.08	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111496427 PE=4 SV=1	[more]
A0A6J1GIG0	2.9e-140	72.19	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111454459 PE=4 SV=1	[more]

BLAST of Tan0006710 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G02300.1	4.8e-87	48.40	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT2G26440.1	1.9e-83	47.44	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT1G02810.1	1.6e-82	46.82	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G43270.1	2.7e-82	46.93	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G33220.1	2.7e-82	46.01	pectin methylesterase 44	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 30..323 e-value: 1.1E-107 score: 359.2
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 26..338 e-value: 1.7E-110 score: 371.3
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 28..337
None	No IPR available	PANTHER	PTHR31707:SF205	PECTINESTERASE/PECTINESTERASE INHIBITOR 22-RELATED	coord: 28..337
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 174..183
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 30..336

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0006710.1	Tan0006710.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity