Tan0002627 (gene) Snake gourd v1

Overview
NameTan0002627
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionCBL-interacting serine/threonine-protein kinase 14-like
LocationLG03: 62846594 .. 62848371 (+)
RNA-Seq ExpressionTan0002627
SyntenyTan0002627
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTCACTCTAAAACTTTCTCCCTTTCTCTCTCCTCAACCTTCTCACCTATTTCCATGGCAGAAAAGCAGTTGAAAAAAAATAATATGCCAAAACCCTATAATTAACACAACCAAACCTCTCCAAGAATCTTCAAAGATCTTCCCCAAATTCTCAAAATCCTCCTTCACCGAATTCCCATGAAGGCAAAGGCAAATCGAGAATTGAATTAAAGTACCAGCTCCTTTTCGTTTGTAACTGTTGCCATGCCGGAGTTCGACGACGGAAATTCTTCGCCGGAACTTGCCGGTGCCGACGGGATTCTGTTCGGAAAATACGAACTCGGGAAGCTTTTGGGATGTGGAGCCTTCGCGAAGGTCTTCCATGGCAGAAACGTTCTTACGGGACAGAGCGTTGCGATTAAAGCCGTTAACAAACTCAAGGTTCTCAAAGGCGGTTTCACGGCGCACGTTAAGAGAGAAATCGCTATCATGCGCCGCCTTCGTCATCCAAACGTCGTTCGTCTCTTCGAAGTTCTCGCCACGAAGAAGAAGATTTATTTCGTCATGGAGTTCGCCAAGGGAGGAGAACTCTTCGCCAAGGTTTCCAAGGGGCGATTCAGTGAGAATCTCAGCCGTCGGTACTTTCAGCAGTTGATCACCGCCGTTGGATACTGTCATTCCAGAGGAATTTTTCATCGCGATTTGAAGCCGGAGAATCTCCTTCTCGATGACAATTTCGATCTCAGAGTTTCGGATTTTGGACTCAGCGCTTTCACCGATCAGATTCGTCCCGACGGATTGCTCCACACGCTCTGTGGAACTCCGGCCTACGTCGCGCCGGAGATTCTCGCCAAGAAAGGCTACGACGGCGCTAAGATCGACGTCTGGTCCTGCGGTATAATCCTCTATGTTCTCACTACTGGATACCTTCCGTTTAATGATTCCAATTTAATGGCGATGTATCGGAAGATTTACAGAGGCGATTTCCGGTGCCCTAAGTGGACGTCGCCAGATCTGCGGCGGTTTCTGTCCAGGCTTCTGGATACGAATCCGGAGACGAGGATCACCGTCGATGAGATTCATCAAGATCCGTGGTTCAGAAAGGGATTTCAGGAGGAGAAGCTTCAATTTGAAGATTATTCTAGTTCAGAGCTTTACGGCGATGTGAACAATTCGAAATGTCTGAATGCTTTTGATCTTATTTCGTTCTCACCTGGCTTTGATTTGACTGGATTGATCAACGAGACGGAATTTTCGTCCGGCGTTGAGAGGTTCCTCTCCGCTGAGTCGCCAGAGAAGATCATTGTGACGGTGGAGGAGGTTTCGAAGTCGGAAAATGCGGCCGTGGAGAAGAACGGCTGGGGAGCTAAAGTGGAAGGCCACGACGGCGGTTTCGTCGCCGTTATTGATATTTTCCGGCTGACGGAGGAATTGGTGGTTGTTGAGATCAAAAACAGAGTGAGGGAAATTGATCAAATTTGGAAAGATAAATTGAGACCTCAACTCGAATGTTTAATCTACCGGTCGGACTGATGCATTTCGATCGAAAGTCGGTGGCCGGTTTCAAGGATTTGATCGGAAATCCGAACGACGAGGACGATGCAGTCGACGGTACGGTTTTAGGAGAATTTTTCCGGGTTTCTTGTAAACGTTTTCTTCTTTCCCGGATTTTTTGAATTTTTTTTTTTTTTTAAATTTCTGTAATTTCTCTTGAATGGGAATTTTGTAATTATACTAACTTTGATGTTTTATATATTTTTATTTTTCATTGTATAATTTATAAATCAAATTAAGCGGAG

mRNA sequence

CCTCACTCTAAAACTTTCTCCCTTTCTCTCTCCTCAACCTTCTCACCTATTTCCATGGCAGAAAAGCAGTTGAAAAAAAATAATATGCCAAAACCCTATAATTAACACAACCAAACCTCTCCAAGAATCTTCAAAGATCTTCCCCAAATTCTCAAAATCCTCCTTCACCGAATTCCCATGAAGGCAAAGGCAAATCGAGAATTGAATTAAAGTACCAGCTCCTTTTCGTTTGTAACTGTTGCCATGCCGGAGTTCGACGACGGAAATTCTTCGCCGGAACTTGCCGGTGCCGACGGGATTCTGTTCGGAAAATACGAACTCGGGAAGCTTTTGGGATGTGGAGCCTTCGCGAAGGTCTTCCATGGCAGAAACGTTCTTACGGGACAGAGCGTTGCGATTAAAGCCGTTAACAAACTCAAGGTTCTCAAAGGCGGTTTCACGGCGCACGTTAAGAGAGAAATCGCTATCATGCGCCGCCTTCGTCATCCAAACGTCGTTCGTCTCTTCGAAGTTCTCGCCACGAAGAAGAAGATTTATTTCGTCATGGAGTTCGCCAAGGGAGGAGAACTCTTCGCCAAGGTTTCCAAGGGGCGATTCAGTGAGAATCTCAGCCGTCGGTACTTTCAGCAGTTGATCACCGCCGTTGGATACTGTCATTCCAGAGGAATTTTTCATCGCGATTTGAAGCCGGAGAATCTCCTTCTCGATGACAATTTCGATCTCAGAGTTTCGGATTTTGGACTCAGCGCTTTCACCGATCAGATTCGTCCCGACGGATTGCTCCACACGCTCTGTGGAACTCCGGCCTACGTCGCGCCGGAGATTCTCGCCAAGAAAGGCTACGACGGCGCTAAGATCGACGTCTGGTCCTGCGGTATAATCCTCTATGTTCTCACTACTGGATACCTTCCGTTTAATGATTCCAATTTAATGGCGATGTATCGGAAGATTTACAGAGGCGATTTCCGGTGCCCTAAGTGGACGTCGCCAGATCTGCGGCGGTTTCTGTCCAGGCTTCTGGATACGAATCCGGAGACGAGGATCACCGTCGATGAGATTCATCAAGATCCGTGGTTCAGAAAGGGATTTCAGGAGGAGAAGCTTCAATTTGAAGATTATTCTAGTTCAGAGCTTTACGGCGATGTGAACAATTCGAAATGTCTGAATGCTTTTGATCTTATTTCGTTCTCACCTGGCTTTGATTTGACTGGATTGATCAACGAGACGGAATTTTCGTCCGGCGTTGAGAGGTTCCTCTCCGCTGAGTCGCCAGAGAAGATCATTGTGACGGTGGAGGAGGTTTCGAAGTCGGAAAATGCGGCCGTGGAGAAGAACGGCTGGGGAGCTAAAGTGGAAGGCCACGACGGCGGTTTCGTCGCCGTTATTGATATTTTCCGGCTGACGGAGGAATTGGTGGTTGTTGAGATCAAAAACAGAGTGAGGGAAATTGATCAAATTTGGAAAGATAAATTGAGACCTCAACTCGAATGTTTAATCTACCGGTCGGACTGATGCATTTCGATCGAAAGTCGGTGGCCGGTTTCAAGGATTTGATCGGAAATCCGAACGACGAGGACGATGCAGTCGACGGTACGGTTTTAGGAGAATTTTTCCGGGTTTCTTGTAAACGTTTTCTTCTTTCCCGGATTTTTTGAATTTTTTTTTTTTTTTAAATTTCTGTAATTTCTCTTGAATGGGAATTTTGTAATTATACTAACTTTGATGTTTTATATATTTTTATTTTTCATTGTATAATTTATAAATCAAATTAAGCGGAG

Coding sequence (CDS)

ATGCCGGAGTTCGACGACGGAAATTCTTCGCCGGAACTTGCCGGTGCCGACGGGATTCTGTTCGGAAAATACGAACTCGGGAAGCTTTTGGGATGTGGAGCCTTCGCGAAGGTCTTCCATGGCAGAAACGTTCTTACGGGACAGAGCGTTGCGATTAAAGCCGTTAACAAACTCAAGGTTCTCAAAGGCGGTTTCACGGCGCACGTTAAGAGAGAAATCGCTATCATGCGCCGCCTTCGTCATCCAAACGTCGTTCGTCTCTTCGAAGTTCTCGCCACGAAGAAGAAGATTTATTTCGTCATGGAGTTCGCCAAGGGAGGAGAACTCTTCGCCAAGGTTTCCAAGGGGCGATTCAGTGAGAATCTCAGCCGTCGGTACTTTCAGCAGTTGATCACCGCCGTTGGATACTGTCATTCCAGAGGAATTTTTCATCGCGATTTGAAGCCGGAGAATCTCCTTCTCGATGACAATTTCGATCTCAGAGTTTCGGATTTTGGACTCAGCGCTTTCACCGATCAGATTCGTCCCGACGGATTGCTCCACACGCTCTGTGGAACTCCGGCCTACGTCGCGCCGGAGATTCTCGCCAAGAAAGGCTACGACGGCGCTAAGATCGACGTCTGGTCCTGCGGTATAATCCTCTATGTTCTCACTACTGGATACCTTCCGTTTAATGATTCCAATTTAATGGCGATGTATCGGAAGATTTACAGAGGCGATTTCCGGTGCCCTAAGTGGACGTCGCCAGATCTGCGGCGGTTTCTGTCCAGGCTTCTGGATACGAATCCGGAGACGAGGATCACCGTCGATGAGATTCATCAAGATCCGTGGTTCAGAAAGGGATTTCAGGAGGAGAAGCTTCAATTTGAAGATTATTCTAGTTCAGAGCTTTACGGCGATGTGAACAATTCGAAATGTCTGAATGCTTTTGATCTTATTTCGTTCTCACCTGGCTTTGATTTGACTGGATTGATCAACGAGACGGAATTTTCGTCCGGCGTTGAGAGGTTCCTCTCCGCTGAGTCGCCAGAGAAGATCATTGTGACGGTGGAGGAGGTTTCGAAGTCGGAAAATGCGGCCGTGGAGAAGAACGGCTGGGGAGCTAAAGTGGAAGGCCACGACGGCGGTTTCGTCGCCGTTATTGATATTTTCCGGCTGACGGAGGAATTGGTGGTTGTTGAGATCAAAAACAGAGTGAGGGAAATTGATCAAATTTGGAAAGATAAATTGAGACCTCAACTCGAATGTTTAATCTACCGGTCGGACTGA

Protein sequence

MPEFDDGNSSPELAGADGILFGKYELGKLLGCGAFAKVFHGRNVLTGQSVAIKAVNKLKVLKGGFTAHVKREIAIMRRLRHPNVVRLFEVLATKKKIYFVMEFAKGGELFAKVSKGRFSENLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSAFTDQIRPDGLLHTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWFRKGFQEEKLQFEDYSSSELYGDVNNSKCLNAFDLISFSPGFDLTGLINETEFSSGVERFLSAESPEKIIVTVEEVSKSENAAVEKNGWGAKVEGHDGGFVAVIDIFRLTEELVVVEIKNRVREIDQIWKDKLRPQLECLIYRSD
Homology
BLAST of Tan0002627 vs. ExPASy Swiss-Prot
Match: Q9LZW4 (CBL-interacting serine/threonine-protein kinase 14 OS=Arabidopsis thaliana OX=3702 GN=CIPK14 PE=1 SV=1)

HSP 1 Score: 549.3 bits (1414), Expect = 3.8e-155
Identity = 268/414 (64.73%), Postives = 330/414 (79.71%), Query Frame = 0

Query: 18  GILFGKYELGKLLGCGAFAKVFHGRNVLTGQSVAIKAVNKLKVLKGGFTAHVKREIAIMR 77
           G LFGKYE+GKL+GCGAFAKV+HGR+  TGQSVAIK V+K ++ KGG   +++REIAIM 
Sbjct: 16  GQLFGKYEVGKLVGCGAFAKVYHGRSTATGQSVAIKVVSKQRLQKGGLNGNIQREIAIMH 75

Query: 78  RLRHPNVVRLFEVLATKKKIYFVMEFAKGGELFAKVSKGRFSENLSRRYFQQLITAVGYC 137
           RLRHP++VRLFEVLATK KI+FVMEFAKGGELFAKVSKGRF E+LSRRYFQQLI+AVGYC
Sbjct: 76  RLRHPSIVRLFEVLATKSKIFFVMEFAKGGELFAKVSKGRFCEDLSRRYFQQLISAVGYC 135

Query: 138 HSRGIFHRDLKPENLLLDDNFDLRVSDFGLSAFTDQIRPDGLLHTLCGTPAYVAPEILAK 197
           HSRGIFHRDLKPENLLLD+  DL++SDFGLSA TDQIRPDGLLHTLCGTPAYVAPE+LAK
Sbjct: 136 HSRGIFHRDLKPENLLLDEKLDLKISDFGLSALTDQIRPDGLLHTLCGTPAYVAPEVLAK 195

Query: 198 KGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDLRRFLSR 257
           KGYDGAKID+WSCGIIL+VL  GYLPFND NLM MYRKIY+G+FR PKWTSPDLRR L+R
Sbjct: 196 KGYDGAKIDIWSCGIILFVLNAGYLPFNDHNLMVMYRKIYKGEFRIPKWTSPDLRRLLTR 255

Query: 258 LLDTNPETRITVDEIHQDPWFRKGFQEEKLQFE-DYSSSELYGDVNNSKC----LNAFDL 317
           LLDTNP+TRIT++EI  DPWF++G+ +   +F  + S  +L  D  +S+     +NAFD+
Sbjct: 256 LLDTNPQTRITIEEIIHDPWFKQGYDDRMSKFHLEDSDMKLPADETDSEMGARRMNAFDI 315

Query: 318 ISFSPGFDLTGLINETEFSSGVERFLSAESPEKIIVTVEEVSKSENAAV-EKNGWGAKVE 377
           IS SPGF+L+GL  +      VERF+SA + E+++  +EE+  +EN  V +K  WG K+E
Sbjct: 316 ISGSPGFNLSGLFGDARKYDRVERFVSAWTAERVVERLEEIVSAENLTVAKKETWGMKIE 375

Query: 378 GHDGGFVAVIDIFRLTEELVVVEIKNRVREI---DQIWKDKLRPQLECLIYRSD 423
           G  G F  V++I +LT+ELV++E++ R R       +W D LRP    L++ SD
Sbjct: 376 GQKGNFAMVVEINQLTDELVMIEVRKRQRAAASGRDLWTDTLRPFFVELVHESD 429

BLAST of Tan0002627 vs. ExPASy Swiss-Prot
Match: O22932 (CBL-interacting serine/threonine-protein kinase 11 OS=Arabidopsis thaliana OX=3702 GN=CIPK11 PE=1 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 3.3e-138
Identity = 258/424 (60.85%), Postives = 320/424 (75.47%), Query Frame = 0

Query: 1   MPEFDDGNSSPELAGADGILFGKYELGKLLGCGAFAKVFHGRNVLTGQSVAIKAVNKLKV 60
           MPE +    S +   A   LFGKYELGKLLGCGAFAKVFH R+  TGQSVA+K +NK K+
Sbjct: 1   MPEIEIAAGSGDNNDA---LFGKYELGKLLGCGAFAKVFHARDRRTGQSVAVKILNKKKL 60

Query: 61  LKGGFTA-HVKREIAIMRRLRHPNVVRLFEVLATKKKIYFVMEFAKGGELFAKVSK-GRF 120
           L     A ++KREI+IMRRL HPN+V+L EV+ATK KI+F MEF KGGELF K+SK GR 
Sbjct: 61  LTNPALANNIKREISIMRRLSHPNIVKLHEVMATKSKIFFAMEFVKGGELFNKISKHGRL 120

Query: 121 SENLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSAFTDQIRPDG 180
           SE+LSRRYFQQLI+AVGYCH+RG++HRDLKPENLL+D+N +L+VSDFGLSA TDQIRPDG
Sbjct: 121 SEDLSRRYFQQLISAVGYCHARGVYHRDLKPENLLIDENGNLKVSDFGLSALTDQIRPDG 180

Query: 181 LLHTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIYR 240
           LLHTLCGTPAYVAPEIL+KKGY+GAK+DVWSCGI+L+VL  GYLPFND N+M MY+KIY+
Sbjct: 181 LLHTLCGTPAYVAPEILSKKGYEGAKVDVWSCGIVLFVLVAGYLPFNDPNVMNMYKKIYK 240

Query: 241 GDFRCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWF-RKGFQEEKLQFEDYSSSEL 300
           G++R P+W SPDL+RF+SRLLD NPETRIT+DEI +DPWF R GF++ K   ++    ++
Sbjct: 241 GEYRFPRWMSPDLKRFVSRLLDINPETRITIDEILKDPWFVRGGFKQIKFHDDEIEDQKV 300

Query: 301 YGDVNNSKCLNAFDLISFSPGFDLTGLINETEFSSG-VERFLSAESPEKIIVTVEEVSKS 360
              +   K LNAFDLIS+S G DL+GL      SSG  ERFLS +SPE +   VE  ++ 
Sbjct: 301 ESSLEAVKSLNAFDLISYSSGLDLSGLFAGCSNSSGESERFLSEKSPEMLAEEVEGFARE 360

Query: 361 ENAAVEK---NGWGAKVEGHDGGFVAVIDIFRLTEELVVVEIKNRVREID---QIWKDKL 415
           EN  ++K     +G ++EG +G F   I I RL + LVVVE + R  + D   ++W  KL
Sbjct: 361 ENLRMKKKKEEEYGFEMEGQNGKFGIGICISRLNDLLVVVEARRRGGDGDCYKEMWNGKL 420

BLAST of Tan0002627 vs. ExPASy Swiss-Prot
Match: O80902 (CBL-interacting serine/threonine-protein kinase 22 OS=Arabidopsis thaliana OX=3702 GN=CIPK22 PE=1 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 9.9e-127
Identity = 239/425 (56.24%), Postives = 303/425 (71.29%), Query Frame = 0

Query: 5   DDGNSSPEL----AGAD--GILFGKYELGKLLGCGAFAKVFHGRNVLT-GQSVAIKAVNK 64
           +D NSS  +     G D    LFGKY+LGKLLG GAFAKV+   ++   G+SVAIK V K
Sbjct: 3   EDSNSSESIIVNVTGDDNKSALFGKYDLGKLLGSGAFAKVYQAEDLQNGGESVAIKVVQK 62

Query: 65  LKVLKGGFTAHVKREIAIMRRLRHPNVVRLFEVLATKKKIYFVMEFAKGGELFAKVSKGR 124
            K LK G TAHVKREI++MRRLRHP++V L EVLATK KIYFVME AKGGELF++V+  R
Sbjct: 63  -KRLKDGLTAHVKREISVMRRLRHPHIVLLSEVLATKTKIYFVMELAKGGELFSRVTSNR 122

Query: 125 FSENLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSAFTDQIRPD 184
           F+E+LSR+YF+QLI+AV YCH+RG+FHRDLKPENLLLD+N DL+VSDFGLSA  +QI PD
Sbjct: 123 FTESLSRKYFRQLISAVRYCHARGVFHRDLKPENLLLDENRDLKVSDFGLSAMKEQIHPD 182

Query: 185 GLLHTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIY 244
           G+LHTLCGTPAYVAPE+L KKGYDG+K D+WSCG++L++L  GYLPF D N+M +YRKI+
Sbjct: 183 GMLHTLCGTPAYVAPELLLKKGYDGSKADIWSCGVVLFLLNAGYLPFRDPNIMGLYRKIH 242

Query: 245 RGDFRCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWFRKGFQEEK---LQFEDYSS 304
           +  ++ P WTS DLR+ L RLL+ NPE RITV+EI +DPWF  G    +   +Q +DY  
Sbjct: 243 KAQYKLPDWTSSDLRKLLRRLLEPNPELRITVEEILKDPWFNHGVDPSEIIGIQADDYDL 302

Query: 305 SELYGDVNNSKCLNAFDLISFSPGFDLTGLINETEFSSGVERFLSAESPEKIIVTVEEVS 364
            E      N K LNAFDLIS +   +L+GL          ++F+S ES   I+  VEEV+
Sbjct: 303 EE------NGKILNAFDLISSASSSNLSGLFGNFVTPDHCDQFVSDESTAVIMRKVEEVA 362

Query: 365 KSENAAVEKNGWGA-KVEGHDGGFVAVIDIFRLTEELVVVEIKNRVREIDQIWKDKLRPQ 419
           K  N  + K    A K+EG  G    V+ + RLT ELV+VE+KN+ R++  +W D LR +
Sbjct: 363 KQLNLRIAKKKERAIKLEGPHGVANVVVKVRRLTNELVMVEMKNKQRDVGLVWADALRQK 420

BLAST of Tan0002627 vs. ExPASy Swiss-Prot
Match: Q9SN43 (CBL-interacting serine/threonine-protein kinase 12 OS=Arabidopsis thaliana OX=3702 GN=CIPK12 PE=1 SV=1)

HSP 1 Score: 452.2 bits (1162), Expect = 6.4e-126
Identity = 233/445 (52.36%), Postives = 303/445 (68.09%), Query Frame = 0

Query: 12  ELAGADGILFGKYELGKLLGCGAFAKVFHGRNVLTGQSVAIKAVNKLKVLKGGFTAHVKR 71
           E +    ++ G+YE+GKLLG G FAKV+  RNV T +SVAIK ++K KVLKGG  AH+KR
Sbjct: 14  ERSSPQALILGRYEMGKLLGHGTFAKVYLARNVKTNESVAIKVIDKEKVLKGGLIAHIKR 73

Query: 72  EIAIMRRLRHPNVVRLFEVLATKKKIYFVMEFAKGGELFAKVSKGRFSENLSRRYFQQLI 131
           EI+I+RR+RHPN+V+LFEV+ATK KIYFVME+ +GGELF KV+KGR  E ++R+YFQQLI
Sbjct: 74  EISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFNKVAKGRLKEEVARKYFQQLI 133

Query: 132 TAVGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSAFTDQIRPDGLLHTLCGTPAYVA 191
           +AV +CH+RG++HRDLKPENLLLD+N +L+VSDFGLSA +DQIR DGL HT CGTPAYVA
Sbjct: 134 SAVTFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTPAYVA 193

Query: 192 PEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDL 251
           PE+LA+KGYD AK+D+WSCG+IL+VL  GYLPF+D N+MAMY+KIYRG+FRCP+W S +L
Sbjct: 194 PEVLARKGYDAAKVDIWSCGVILFVLMAGYLPFHDRNVMAMYKKIYRGEFRCPRWFSTEL 253

Query: 252 RRFLSRLLDTNPETRITVDEIHQDPWFRKGFQEEKLQFED-------------------- 311
            R LS+LL+TNPE R T  EI ++ WF+KGF+  K   ED                    
Sbjct: 254 TRLLSKLLETNPEKRFTFPEIMENSWFKKGFKHIKFYVEDDKLCNVVDDDELESDSVESD 313

Query: 312 ----YSSSEL--------YGDVNNSKCLNAFDLISFSPGFDLTGLINETEFSSGVERFLS 371
                S SE+         G +     LNAFD+ISFS GFDL+GL ++    S   RF+S
Sbjct: 314 RDSAASESEIEYLEPRRRVGGLPRPASLNAFDIISFSQGFDLSGLFDDDGEGS---RFVS 373

Query: 372 AESPEKIIVTVEEVSKSENAAVEKNGWGAKVEGH----DGGFVAVIDIFRLTEELVVVEI 418
                KII  +EE++K  +  V K      +EG      G      +IF LT  LVVVE+
Sbjct: 374 GAPVSKIISKLEEIAKVVSFTVRKKDCRVSLEGSRQGVKGPLTIAAEIFELTPSLVVVEV 433

BLAST of Tan0002627 vs. ExPASy Swiss-Prot
Match: Q68Y49 (CBL-interacting protein kinase 19 OS=Oryza sativa subsp. japonica OX=39947 GN=CIPK19 PE=1 SV=1)

HSP 1 Score: 451.1 bits (1159), Expect = 1.4e-125
Identity = 236/480 (49.17%), Postives = 305/480 (63.54%), Query Frame = 0

Query: 9   SSPELAG---ADGILFGKYELGKLLGCGAFAKVFHGRNVLTGQSVAIKAVNKLKVLKGGF 68
           S+  LAG     G+L G+YELG+LLG G FAKV+  R+  +G+ VAIK ++K K ++ G 
Sbjct: 19  SAASLAGKPRGGGLLLGRYELGRLLGHGTFAKVYQARSADSGEPVAIKVLDKEKAMRHGL 78

Query: 69  TAHVKREIAIMRRLRHPNVVRLFEVLATKKKIYFVMEFAKGGELFAKVSKGRFSENLSRR 128
             H+KREIAI+RR+RHPN+VRLFEV+ATK KIYFVME  +GGELF +V+KGR  E+ +RR
Sbjct: 79  VPHIKREIAILRRVRHPNIVRLFEVMATKSKIYFVMELVRGGELFGRVAKGRLKEDTARR 138

Query: 129 YFQQLITAVGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSAFTDQIRPDGLLHTLCG 188
           YFQQL++AVG+CH+RG+FHRDLKPENLL+D++ DL+VSDFGLSA  DQ  PDGLLHT CG
Sbjct: 139 YFQQLVSAVGFCHARGVFHRDLKPENLLVDEHGDLKVSDFGLSAVADQFHPDGLLHTFCG 198

Query: 189 TPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIYRGDFRCPK 248
           TP+YVAPE+LA++GYDGAK D+WSCGIIL+VL  GYLPF+D NLMAMYRKIYRG+FRCP+
Sbjct: 199 TPSYVAPEVLARRGYDGAKADIWSCGIILFVLMAGYLPFHDQNLMAMYRKIYRGEFRCPR 258

Query: 249 WTSPDLRRFLSRLLDTNPETRITVDEIHQDPWFRKGFQEEKLQFED-------------- 308
           W S DL   L+R+LDTNPETRITV E+ +  WF+KGF+  +   ED              
Sbjct: 259 WFSKDLSSLLNRILDTNPETRITVKEVMESRWFQKGFRPVRFYVEDDQVHSLADGDNDMP 318

Query: 309 -----------------------------------------YSSSEL----YGDVNNSKC 368
                                                     SS E      G +     
Sbjct: 319 ELEPSEPPPPPPFPPPPPQQDDDGEESGWESDSSVASCPATLSSEERRQRPLGSLTRPAS 378

Query: 369 LNAFDLISFSPGFDLTGLINETEFSSGVERFLSAESPEKIIVTVEEVSKSENAAVEKNGW 420
           LNAFD+ISFS GFDL+GL  E        RF+SAE  + II  +EE++K ++  V +  W
Sbjct: 379 LNAFDIISFSKGFDLSGLFEE---RGSEVRFISAEPMQTIITKLEEIAKVKSFFVRRKDW 438

BLAST of Tan0002627 vs. NCBI nr
Match: XP_022949666.1 (CBL-interacting serine/threonine-protein kinase 14-like [Cucurbita moschata])

HSP 1 Score: 783.9 bits (2023), Expect = 7.1e-223
Identity = 395/429 (92.07%), Postives = 404/429 (94.17%), Query Frame = 0

Query: 1   MPEFDDGNSSPELAGAD----GILFGKYELGKLLGCGAFAKVFHGRNVLTGQSVAIKAVN 60
           MP FDD N  P LA  D    GILFGKYELGKLLGCGAFAKV+HGRNVLTGQSVAIK VN
Sbjct: 1   MPPFDD-NPPPRLARGDSLPEGILFGKYELGKLLGCGAFAKVYHGRNVLTGQSVAIKIVN 60

Query: 61  KLKVLKGGFTAHVKREIAIMRRLRHPNVVRLFEVLATKKKIYFVMEFAKGGELFAKVSKG 120
           KLKVLKGGF AHVKREIAIMRRLRHPNVVRLFEVLATK KIYFVMEFAKGGELF KVSKG
Sbjct: 61  KLKVLKGGFKAHVKREIAIMRRLRHPNVVRLFEVLATKTKIYFVMEFAKGGELFGKVSKG 120

Query: 121 RFSENLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSAFTDQIRP 180
           RFSENLSRRYFQQLITAVGYCHS+GIFHRDLKPENLLLDDNFDLRVSDFGLSA TDQIRP
Sbjct: 121 RFSENLSRRYFQQLITAVGYCHSQGIFHRDLKPENLLLDDNFDLRVSDFGLSALTDQIRP 180

Query: 181 DGLLHTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKI 240
           DGLLHTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKI
Sbjct: 181 DGLLHTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKI 240

Query: 241 YRGDFRCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWFRKGFQEEKLQFEDYSSSE 300
           YRGDFRCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWF KGFQEEKLQ +D S+SE
Sbjct: 241 YRGDFRCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWFVKGFQEEKLQIDD-SASE 300

Query: 301 LYGDVNNSKCLNAFDLISFSPGFDLTGLINETEFSSGVERFLSAESPEKIIVTVEEVSKS 360
           +YGDVNN+KCLNAFDLISFSPGFDLTGLINETEFSSGVERFLSAESPEKIIVTVEE SKS
Sbjct: 301 IYGDVNNAKCLNAFDLISFSPGFDLTGLINETEFSSGVERFLSAESPEKIIVTVEEASKS 360

Query: 361 ENAAVEKNGWGAKVEGHDGGFVAVIDIFRLTEELVVVEIKNRVREID---QIWKDKLRPQ 420
           ENAAVEKNGWGAKVEG DGGF+AVIDIFRLTEELVVVEIKNRVRE+D   QIWKD LRPQ
Sbjct: 361 ENAAVEKNGWGAKVEGLDGGFIAVIDIFRLTEELVVVEIKNRVREVDQDGQIWKDILRPQ 420

Query: 421 LECLIYRSD 423
           LECLIYRS+
Sbjct: 421 LECLIYRSE 427

BLAST of Tan0002627 vs. NCBI nr
Match: XP_023545198.1 (CBL-interacting serine/threonine-protein kinase 14-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 783.1 bits (2021), Expect = 1.2e-222
Identity = 395/429 (92.07%), Postives = 402/429 (93.71%), Query Frame = 0

Query: 1   MPEFDDGNSSPELAGAD----GILFGKYELGKLLGCGAFAKVFHGRNVLTGQSVAIKAVN 60
           MP FDD N  P L   D    GILFGKYELGKLLGCGAFAKV+HGRNVLTGQSVAIK VN
Sbjct: 1   MPPFDD-NPPPRLVRGDSLPEGILFGKYELGKLLGCGAFAKVYHGRNVLTGQSVAIKIVN 60

Query: 61  KLKVLKGGFTAHVKREIAIMRRLRHPNVVRLFEVLATKKKIYFVMEFAKGGELFAKVSKG 120
           KLKVLKGGF AHVKREIAIMRRLRHPNVVRLFEVLATK KIYFVMEFAKGGELF KVSKG
Sbjct: 61  KLKVLKGGFKAHVKREIAIMRRLRHPNVVRLFEVLATKTKIYFVMEFAKGGELFGKVSKG 120

Query: 121 RFSENLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSAFTDQIRP 180
           RFSENLSRRYFQQLITAVGYCHS+GIFHRDLKPENLLLDDNFDLRVSDFGLSA TDQIRP
Sbjct: 121 RFSENLSRRYFQQLITAVGYCHSQGIFHRDLKPENLLLDDNFDLRVSDFGLSALTDQIRP 180

Query: 181 DGLLHTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKI 240
           DGLLHTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKI
Sbjct: 181 DGLLHTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKI 240

Query: 241 YRGDFRCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWFRKGFQEEKLQFEDYSSSE 300
           YRGDFRCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWF KGFQEEKLQ ED S SE
Sbjct: 241 YRGDFRCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWFVKGFQEEKLQIED-SGSE 300

Query: 301 LYGDVNNSKCLNAFDLISFSPGFDLTGLINETEFSSGVERFLSAESPEKIIVTVEEVSKS 360
           +YGDVNN+KCLNAFDLISFSPGFDLTGLINETEFSSGVERFLSAESPEKIIVTVEE SKS
Sbjct: 301 IYGDVNNAKCLNAFDLISFSPGFDLTGLINETEFSSGVERFLSAESPEKIIVTVEEASKS 360

Query: 361 ENAAVEKNGWGAKVEGHDGGFVAVIDIFRLTEELVVVEIKNRVREID---QIWKDKLRPQ 420
           ENAAVEKNGWGAKVEG DGGF+AVIDIFRLTEELVVVEIKNRVRE+D   QIWKD LRPQ
Sbjct: 361 ENAAVEKNGWGAKVEGLDGGFIAVIDIFRLTEELVVVEIKNRVREVDPDGQIWKDILRPQ 420

Query: 421 LECLIYRSD 423
           LECLIYRS+
Sbjct: 421 LECLIYRSE 427

BLAST of Tan0002627 vs. NCBI nr
Match: KAG7033738.1 (CBL-interacting serine/threonine-protein kinase 14, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 782.7 bits (2020), Expect = 1.6e-222
Identity = 395/428 (92.29%), Postives = 403/428 (94.16%), Query Frame = 0

Query: 2   PEFDDGNSSPELAGAD----GILFGKYELGKLLGCGAFAKVFHGRNVLTGQSVAIKAVNK 61
           P FDD N  P LA  D    GILFGKYELGKLLGCGAFAKV+HGRNVLTGQSVAIK VNK
Sbjct: 9   PPFDD-NPPPRLARGDSLPEGILFGKYELGKLLGCGAFAKVYHGRNVLTGQSVAIKIVNK 68

Query: 62  LKVLKGGFTAHVKREIAIMRRLRHPNVVRLFEVLATKKKIYFVMEFAKGGELFAKVSKGR 121
           LKVLKGGF AHVKREIAIMRRLRHPNVVRLFEVLATK KIYFVMEFAKGGELF KVSKGR
Sbjct: 69  LKVLKGGFKAHVKREIAIMRRLRHPNVVRLFEVLATKTKIYFVMEFAKGGELFGKVSKGR 128

Query: 122 FSENLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSAFTDQIRPD 181
           FSENLSRRYFQQLITAVGYCHS+GIFHRDLKPENLLLDDNFDLRVSDFGLSA TDQIRPD
Sbjct: 129 FSENLSRRYFQQLITAVGYCHSQGIFHRDLKPENLLLDDNFDLRVSDFGLSALTDQIRPD 188

Query: 182 GLLHTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIY 241
           GLLHTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIY
Sbjct: 189 GLLHTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIY 248

Query: 242 RGDFRCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWFRKGFQEEKLQFEDYSSSEL 301
           RGDFRCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWF KGFQEEKLQ ED S+SE+
Sbjct: 249 RGDFRCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWFVKGFQEEKLQIED-SASEI 308

Query: 302 YGDVNNSKCLNAFDLISFSPGFDLTGLINETEFSSGVERFLSAESPEKIIVTVEEVSKSE 361
           YGDVNN+KCLNAFDLISFSPGFDLTGLINETEFSSGVERFLSAESPEKIIVTVEE SKSE
Sbjct: 309 YGDVNNAKCLNAFDLISFSPGFDLTGLINETEFSSGVERFLSAESPEKIIVTVEEASKSE 368

Query: 362 NAAVEKNGWGAKVEGHDGGFVAVIDIFRLTEELVVVEIKNRVREID---QIWKDKLRPQL 421
           NAAVEKNGWGAKVEG DGGF+AVIDIFRLTEELVVVEIKNRVRE+D   QIWKD LRPQL
Sbjct: 369 NAAVEKNGWGAKVEGLDGGFIAVIDIFRLTEELVVVEIKNRVREVDQDGQIWKDILRPQL 428

Query: 422 ECLIYRSD 423
           ECLIYRS+
Sbjct: 429 ECLIYRSE 434

BLAST of Tan0002627 vs. NCBI nr
Match: XP_022924879.1 (CBL-interacting serine/threonine-protein kinase 14-like [Cucurbita moschata])

HSP 1 Score: 781.2 bits (2016), Expect = 4.6e-222
Identity = 387/422 (91.71%), Postives = 401/422 (95.02%), Query Frame = 0

Query: 1   MPEFDDGNSSPELAGADGILFGKYELGKLLGCGAFAKVFHGRNVLTGQSVAIKAVNKLKV 60
           MPEFD G+SSPE     GILFGKYELGKLLG GAFAKV+HGRNV TGQSVAIKAVNKLKV
Sbjct: 1   MPEFDGGDSSPE-----GILFGKYELGKLLGRGAFAKVYHGRNVHTGQSVAIKAVNKLKV 60

Query: 61  LKGGFTAHVKREIAIMRRLRHPNVVRLFEVLATKKKIYFVMEFAKGGELFAKVSKGRFSE 120
           L GGFTAHVKREI IMRRLRHPNVVRLFEVLATK KIYFVMEFAKGGELF KVSKGRFSE
Sbjct: 61  LNGGFTAHVKREIDIMRRLRHPNVVRLFEVLATKTKIYFVMEFAKGGELFTKVSKGRFSE 120

Query: 121 NLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSAFTDQIRPDGLL 180
           NLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLD+NFDLRVSDFGL A TDQIRPDGLL
Sbjct: 121 NLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLDENFDLRVSDFGLGALTDQIRPDGLL 180

Query: 181 HTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIYRGD 240
           HTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLT G+LPFNDSNLMAMYRKIYRGD
Sbjct: 181 HTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTAGFLPFNDSNLMAMYRKIYRGD 240

Query: 241 FRCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWFRKGFQEEKLQFEDYSSSELYGD 300
           FRCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWFRKGFQEEK+QFEDYS SELYGD
Sbjct: 241 FRCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWFRKGFQEEKIQFEDYSVSELYGD 300

Query: 301 VNNSKCLNAFDLISFSPGFDLTGLINETEFSSGVERFLSAESPEKIIVTVEEVSKSENAA 360
           +NN+KCLNAFDLISFS  FD TGLINETEFSS VERFLSAESPEKII+TVEEVSKSENAA
Sbjct: 301 MNNAKCLNAFDLISFSLCFDSTGLINETEFSSDVERFLSAESPEKIIMTVEEVSKSENAA 360

Query: 361 VEKNGWGAKVEGHDGGFVAVIDIFRLTEELVVVEIKNRVREIDQIWKDKLRPQLECLIYR 420
           VEKNGWG +VEGHDGGFVAVI+IFRLTEELVVVEIKNRVREIDQIWK+KLRPQL+CLIY+
Sbjct: 361 VEKNGWGVRVEGHDGGFVAVIEIFRLTEELVVVEIKNRVREIDQIWKEKLRPQLDCLIYQ 417

Query: 421 SD 423
           S+
Sbjct: 421 SE 417

BLAST of Tan0002627 vs. NCBI nr
Match: XP_023517341.1 (CBL-interacting serine/threonine-protein kinase 14-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 779.2 bits (2011), Expect = 1.7e-221
Identity = 386/422 (91.47%), Postives = 401/422 (95.02%), Query Frame = 0

Query: 1   MPEFDDGNSSPELAGADGILFGKYELGKLLGCGAFAKVFHGRNVLTGQSVAIKAVNKLKV 60
           MPEFD G+ SPE     GILFGKYELGKLLG GAFAKV+HGRNV TG+SVAIKAVNKLKV
Sbjct: 1   MPEFDGGDPSPE-----GILFGKYELGKLLGRGAFAKVYHGRNVHTGKSVAIKAVNKLKV 60

Query: 61  LKGGFTAHVKREIAIMRRLRHPNVVRLFEVLATKKKIYFVMEFAKGGELFAKVSKGRFSE 120
           L GGFTAHVKREI IMRRLRHPNVVRLFEVLATK KIYFVMEFAKGGELF KVSKGRFSE
Sbjct: 61  LNGGFTAHVKREIDIMRRLRHPNVVRLFEVLATKTKIYFVMEFAKGGELFTKVSKGRFSE 120

Query: 121 NLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSAFTDQIRPDGLL 180
           NLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLD+NFDLRVSDFGL A TDQIRPDGLL
Sbjct: 121 NLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLDENFDLRVSDFGLGALTDQIRPDGLL 180

Query: 181 HTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIYRGD 240
           HTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLT G+LPFNDSNLMAMYRKIYRGD
Sbjct: 181 HTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTAGFLPFNDSNLMAMYRKIYRGD 240

Query: 241 FRCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWFRKGFQEEKLQFEDYSSSELYGD 300
           FRCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWFRKGFQEEK+QFEDYS SELYGD
Sbjct: 241 FRCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWFRKGFQEEKIQFEDYSVSELYGD 300

Query: 301 VNNSKCLNAFDLISFSPGFDLTGLINETEFSSGVERFLSAESPEKIIVTVEEVSKSENAA 360
           +NN+KCLNAFDLISFS  FD TGLINETEFSS VERFLSAESPEKII+TVEEVSKSENAA
Sbjct: 301 MNNAKCLNAFDLISFSLCFDSTGLINETEFSSDVERFLSAESPEKIIMTVEEVSKSENAA 360

Query: 361 VEKNGWGAKVEGHDGGFVAVIDIFRLTEELVVVEIKNRVREIDQIWKDKLRPQLECLIYR 420
           VEKNGWGA+VEGHDGGFVAVI+IFRLTEELVVVEIKNRVREIDQIWK+KLRPQL+CLIY+
Sbjct: 361 VEKNGWGARVEGHDGGFVAVIEIFRLTEELVVVEIKNRVREIDQIWKEKLRPQLDCLIYQ 417

Query: 421 SD 423
           S+
Sbjct: 421 SE 417

BLAST of Tan0002627 vs. ExPASy TrEMBL
Match: A0A6J1GCM3 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111452989 PE=3 SV=1)

HSP 1 Score: 783.9 bits (2023), Expect = 3.4e-223
Identity = 395/429 (92.07%), Postives = 404/429 (94.17%), Query Frame = 0

Query: 1   MPEFDDGNSSPELAGAD----GILFGKYELGKLLGCGAFAKVFHGRNVLTGQSVAIKAVN 60
           MP FDD N  P LA  D    GILFGKYELGKLLGCGAFAKV+HGRNVLTGQSVAIK VN
Sbjct: 1   MPPFDD-NPPPRLARGDSLPEGILFGKYELGKLLGCGAFAKVYHGRNVLTGQSVAIKIVN 60

Query: 61  KLKVLKGGFTAHVKREIAIMRRLRHPNVVRLFEVLATKKKIYFVMEFAKGGELFAKVSKG 120
           KLKVLKGGF AHVKREIAIMRRLRHPNVVRLFEVLATK KIYFVMEFAKGGELF KVSKG
Sbjct: 61  KLKVLKGGFKAHVKREIAIMRRLRHPNVVRLFEVLATKTKIYFVMEFAKGGELFGKVSKG 120

Query: 121 RFSENLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSAFTDQIRP 180
           RFSENLSRRYFQQLITAVGYCHS+GIFHRDLKPENLLLDDNFDLRVSDFGLSA TDQIRP
Sbjct: 121 RFSENLSRRYFQQLITAVGYCHSQGIFHRDLKPENLLLDDNFDLRVSDFGLSALTDQIRP 180

Query: 181 DGLLHTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKI 240
           DGLLHTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKI
Sbjct: 181 DGLLHTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKI 240

Query: 241 YRGDFRCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWFRKGFQEEKLQFEDYSSSE 300
           YRGDFRCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWF KGFQEEKLQ +D S+SE
Sbjct: 241 YRGDFRCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWFVKGFQEEKLQIDD-SASE 300

Query: 301 LYGDVNNSKCLNAFDLISFSPGFDLTGLINETEFSSGVERFLSAESPEKIIVTVEEVSKS 360
           +YGDVNN+KCLNAFDLISFSPGFDLTGLINETEFSSGVERFLSAESPEKIIVTVEE SKS
Sbjct: 301 IYGDVNNAKCLNAFDLISFSPGFDLTGLINETEFSSGVERFLSAESPEKIIVTVEEASKS 360

Query: 361 ENAAVEKNGWGAKVEGHDGGFVAVIDIFRLTEELVVVEIKNRVREID---QIWKDKLRPQ 420
           ENAAVEKNGWGAKVEG DGGF+AVIDIFRLTEELVVVEIKNRVRE+D   QIWKD LRPQ
Sbjct: 361 ENAAVEKNGWGAKVEGLDGGFIAVIDIFRLTEELVVVEIKNRVREVDQDGQIWKDILRPQ 420

Query: 421 LECLIYRSD 423
           LECLIYRS+
Sbjct: 421 LECLIYRSE 427

BLAST of Tan0002627 vs. ExPASy TrEMBL
Match: A0A6J1EAG3 (CBL-interacting serine/threonine-protein kinase 14-like OS=Cucurbita moschata OX=3662 GN=LOC111432298 PE=3 SV=1)

HSP 1 Score: 781.2 bits (2016), Expect = 2.2e-222
Identity = 387/422 (91.71%), Postives = 401/422 (95.02%), Query Frame = 0

Query: 1   MPEFDDGNSSPELAGADGILFGKYELGKLLGCGAFAKVFHGRNVLTGQSVAIKAVNKLKV 60
           MPEFD G+SSPE     GILFGKYELGKLLG GAFAKV+HGRNV TGQSVAIKAVNKLKV
Sbjct: 1   MPEFDGGDSSPE-----GILFGKYELGKLLGRGAFAKVYHGRNVHTGQSVAIKAVNKLKV 60

Query: 61  LKGGFTAHVKREIAIMRRLRHPNVVRLFEVLATKKKIYFVMEFAKGGELFAKVSKGRFSE 120
           L GGFTAHVKREI IMRRLRHPNVVRLFEVLATK KIYFVMEFAKGGELF KVSKGRFSE
Sbjct: 61  LNGGFTAHVKREIDIMRRLRHPNVVRLFEVLATKTKIYFVMEFAKGGELFTKVSKGRFSE 120

Query: 121 NLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSAFTDQIRPDGLL 180
           NLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLD+NFDLRVSDFGL A TDQIRPDGLL
Sbjct: 121 NLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLDENFDLRVSDFGLGALTDQIRPDGLL 180

Query: 181 HTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIYRGD 240
           HTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLT G+LPFNDSNLMAMYRKIYRGD
Sbjct: 181 HTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTAGFLPFNDSNLMAMYRKIYRGD 240

Query: 241 FRCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWFRKGFQEEKLQFEDYSSSELYGD 300
           FRCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWFRKGFQEEK+QFEDYS SELYGD
Sbjct: 241 FRCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWFRKGFQEEKIQFEDYSVSELYGD 300

Query: 301 VNNSKCLNAFDLISFSPGFDLTGLINETEFSSGVERFLSAESPEKIIVTVEEVSKSENAA 360
           +NN+KCLNAFDLISFS  FD TGLINETEFSS VERFLSAESPEKII+TVEEVSKSENAA
Sbjct: 301 MNNAKCLNAFDLISFSLCFDSTGLINETEFSSDVERFLSAESPEKIIMTVEEVSKSENAA 360

Query: 361 VEKNGWGAKVEGHDGGFVAVIDIFRLTEELVVVEIKNRVREIDQIWKDKLRPQLECLIYR 420
           VEKNGWG +VEGHDGGFVAVI+IFRLTEELVVVEIKNRVREIDQIWK+KLRPQL+CLIY+
Sbjct: 361 VEKNGWGVRVEGHDGGFVAVIEIFRLTEELVVVEIKNRVREIDQIWKEKLRPQLDCLIYQ 417

Query: 421 SD 423
           S+
Sbjct: 421 SE 417

BLAST of Tan0002627 vs. ExPASy TrEMBL
Match: A0A6J1ITF4 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111478246 PE=3 SV=1)

HSP 1 Score: 779.2 bits (2011), Expect = 8.4e-222
Identity = 393/429 (91.61%), Postives = 401/429 (93.47%), Query Frame = 0

Query: 1   MPEFDDGNSSPELAGAD----GILFGKYELGKLLGCGAFAKVFHGRNVLTGQSVAIKAVN 60
           MP FDD N  P LA  D    GILFGKYELGKLLGCGAFAKV+HGRNVLTGQSVAIK VN
Sbjct: 1   MPPFDD-NPPPRLARGDSLPEGILFGKYELGKLLGCGAFAKVYHGRNVLTGQSVAIKIVN 60

Query: 61  KLKVLKGGFTAHVKREIAIMRRLRHPNVVRLFEVLATKKKIYFVMEFAKGGELFAKVSKG 120
           KLKVLKGGF AHVKREIAIMRRLRHPNVVRLFEVLATK KIYFVMEFAKGGELF KVSKG
Sbjct: 61  KLKVLKGGFKAHVKREIAIMRRLRHPNVVRLFEVLATKTKIYFVMEFAKGGELFGKVSKG 120

Query: 121 RFSENLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSAFTDQIRP 180
           RFSENLSRRYFQQLITAVGYCHS+GIFHRDLKPENLLLDDNFDLRVSDFGLSA TDQIRP
Sbjct: 121 RFSENLSRRYFQQLITAVGYCHSQGIFHRDLKPENLLLDDNFDLRVSDFGLSALTDQIRP 180

Query: 181 DGLLHTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKI 240
           DGLLHTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKI
Sbjct: 181 DGLLHTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKI 240

Query: 241 YRGDFRCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWFRKGFQEEKLQFEDYSSSE 300
           YRGDFRCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWF KG QEEKLQ ED S SE
Sbjct: 241 YRGDFRCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWFVKGLQEEKLQIED-SGSE 300

Query: 301 LYGDVNNSKCLNAFDLISFSPGFDLTGLINETEFSSGVERFLSAESPEKIIVTVEEVSKS 360
           +YGDVNN+KCLNAFDLISFSPGFDLTGLINETEFSSGVERFLSAESPEKIIVTVEE SKS
Sbjct: 301 IYGDVNNAKCLNAFDLISFSPGFDLTGLINETEFSSGVERFLSAESPEKIIVTVEEASKS 360

Query: 361 ENAAVEKNGWGAKVEGHDGGFVAVIDIFRLTEELVVVEIKNRVREID---QIWKDKLRPQ 420
           ENAAVEKNGWGAKVEG DGGF+ VIDIF+LTEELVVVEIKNRVRE+D   QIWKD LRPQ
Sbjct: 361 ENAAVEKNGWGAKVEGLDGGFIVVIDIFQLTEELVVVEIKNRVREVDQDGQIWKDILRPQ 420

Query: 421 LECLIYRSD 423
           LECLIYRS+
Sbjct: 421 LECLIYRSE 427

BLAST of Tan0002627 vs. ExPASy TrEMBL
Match: A0A6J1HRC5 (CBL-interacting serine/threonine-protein kinase 14-like OS=Cucurbita maxima OX=3661 GN=LOC111466020 PE=3 SV=1)

HSP 1 Score: 774.6 bits (1999), Expect = 2.1e-220
Identity = 384/422 (91.00%), Postives = 399/422 (94.55%), Query Frame = 0

Query: 1   MPEFDDGNSSPELAGADGILFGKYELGKLLGCGAFAKVFHGRNVLTGQSVAIKAVNKLKV 60
           MPEFD G+SS E     GILFGKYELGKLLG GAFAKV+HGRNV TGQSVAIKAVNKLKV
Sbjct: 1   MPEFDGGDSSSE-----GILFGKYELGKLLGRGAFAKVYHGRNVHTGQSVAIKAVNKLKV 60

Query: 61  LKGGFTAHVKREIAIMRRLRHPNVVRLFEVLATKKKIYFVMEFAKGGELFAKVSKGRFSE 120
           L GGFTAHVKREI IMRRLRHPNVVRLFEVLATK KIYFVMEFAKGGELF KVSKGRFSE
Sbjct: 61  LNGGFTAHVKREIDIMRRLRHPNVVRLFEVLATKTKIYFVMEFAKGGELFTKVSKGRFSE 120

Query: 121 NLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSAFTDQIRPDGLL 180
           NLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLD+NFDLRVSDFGL A TDQIRPDGLL
Sbjct: 121 NLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLDENFDLRVSDFGLGALTDQIRPDGLL 180

Query: 181 HTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIYRGD 240
           HTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLT G+LPFNDSNLMAMYRKIYRGD
Sbjct: 181 HTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTAGFLPFNDSNLMAMYRKIYRGD 240

Query: 241 FRCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWFRKGFQEEKLQFEDYSSSELYGD 300
           FRCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWFRKGF EEK+QFEDYS SELYGD
Sbjct: 241 FRCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWFRKGFHEEKIQFEDYSVSELYGD 300

Query: 301 VNNSKCLNAFDLISFSPGFDLTGLINETEFSSGVERFLSAESPEKIIVTVEEVSKSENAA 360
           +NN+KCLNAFDLISFS  FD TGLINETEFSS VERFLSAESPEKII+TV EVSKSENAA
Sbjct: 301 MNNAKCLNAFDLISFSLCFDSTGLINETEFSSDVERFLSAESPEKIIMTVAEVSKSENAA 360

Query: 361 VEKNGWGAKVEGHDGGFVAVIDIFRLTEELVVVEIKNRVREIDQIWKDKLRPQLECLIYR 420
           VEKNGWGAKVEGHDGGF+AVI+IFRLTEELVVVEIKNRVREIDQIWK+KLRPQL+CLI++
Sbjct: 361 VEKNGWGAKVEGHDGGFIAVIEIFRLTEELVVVEIKNRVREIDQIWKEKLRPQLDCLIFQ 417

Query: 421 SD 423
           S+
Sbjct: 421 SE 417

BLAST of Tan0002627 vs. ExPASy TrEMBL
Match: A0A0A0L2V2 (Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_4G331590 PE=3 SV=1)

HSP 1 Score: 759.2 bits (1959), Expect = 9.0e-216
Identity = 385/432 (89.12%), Postives = 398/432 (92.13%), Query Frame = 0

Query: 1   MPEFDDGNSSPELAGAD----GILFGKYELGKLLGCGAFAKVFHGRNVLTGQSVAIKAVN 60
           MP F D    P L  AD    GILFGKYELGKLLGCGAFAKV+HGRNVLTGQSVAIKAVN
Sbjct: 1   MPAFHDDTPPPGLPRADSLPEGILFGKYELGKLLGCGAFAKVYHGRNVLTGQSVAIKAVN 60

Query: 61  KLKVLKGGFTAHVKREIAIMRRLRHPNVVRLFEVLATKKKIYFVMEFAKGGELFAKVSKG 120
           KLKVLKGGFTAHVKREIAIMRRLRHPNVVRLFEVLATK KIYFVME+AKGGELF KVSKG
Sbjct: 61  KLKVLKGGFTAHVKREIAIMRRLRHPNVVRLFEVLATKTKIYFVMEYAKGGELFGKVSKG 120

Query: 121 RFSENLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSAFTDQIRP 180
           RFSENLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSA  DQIRP
Sbjct: 121 RFSENLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSALADQIRP 180

Query: 181 DGLLHTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKI 240
           DGLLHTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLT GYLPFNDSNLMAMYRKI
Sbjct: 181 DGLLHTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTAGYLPFNDSNLMAMYRKI 240

Query: 241 YRGDFRCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWFRKGFQEEKLQFE--DYSS 300
           YRGDFRCPKWTSPDL+RF+SRLLDTNPE+RITVDEI +DPWF KGFQEEK QF+  D S+
Sbjct: 241 YRGDFRCPKWTSPDLQRFISRLLDTNPESRITVDEIQRDPWFVKGFQEEKFQFQELDDSA 300

Query: 301 SELYGDVNNSKCLNAFDLISFSPGFDLTGLI-NETEFSSGVERFLSAESPEKIIVTVEEV 360
           S++YGDVNN+KCLNAFDLISFSPGFDLTGLI  ETEFSS VERFLSAESPEKIIVTVEEV
Sbjct: 301 SDVYGDVNNAKCLNAFDLISFSPGFDLTGLIKEETEFSSCVERFLSAESPEKIIVTVEEV 360

Query: 361 SKSENAAVEKNGWGAKVEGHDGGFVAVIDIFRLTEELVVVEIKNRVREID---QIWKDKL 420
           +KSENAAVEKNGWG KVEG DGGFV VIDIFRLTEELVVVEIKNRVREI+   QIWKD L
Sbjct: 361 TKSENAAVEKNGWGMKVEGLDGGFVVVIDIFRLTEELVVVEIKNRVREIEQDRQIWKDVL 420

Query: 421 RPQLECLIYRSD 423
           RPQLECLIYRSD
Sbjct: 421 RPQLECLIYRSD 432

BLAST of Tan0002627 vs. TAIR 10
Match: AT5G01820.1 (serine/threonine protein kinase 1 )

HSP 1 Score: 549.3 bits (1414), Expect = 2.7e-156
Identity = 268/414 (64.73%), Postives = 330/414 (79.71%), Query Frame = 0

Query: 18  GILFGKYELGKLLGCGAFAKVFHGRNVLTGQSVAIKAVNKLKVLKGGFTAHVKREIAIMR 77
           G LFGKYE+GKL+GCGAFAKV+HGR+  TGQSVAIK V+K ++ KGG   +++REIAIM 
Sbjct: 16  GQLFGKYEVGKLVGCGAFAKVYHGRSTATGQSVAIKVVSKQRLQKGGLNGNIQREIAIMH 75

Query: 78  RLRHPNVVRLFEVLATKKKIYFVMEFAKGGELFAKVSKGRFSENLSRRYFQQLITAVGYC 137
           RLRHP++VRLFEVLATK KI+FVMEFAKGGELFAKVSKGRF E+LSRRYFQQLI+AVGYC
Sbjct: 76  RLRHPSIVRLFEVLATKSKIFFVMEFAKGGELFAKVSKGRFCEDLSRRYFQQLISAVGYC 135

Query: 138 HSRGIFHRDLKPENLLLDDNFDLRVSDFGLSAFTDQIRPDGLLHTLCGTPAYVAPEILAK 197
           HSRGIFHRDLKPENLLLD+  DL++SDFGLSA TDQIRPDGLLHTLCGTPAYVAPE+LAK
Sbjct: 136 HSRGIFHRDLKPENLLLDEKLDLKISDFGLSALTDQIRPDGLLHTLCGTPAYVAPEVLAK 195

Query: 198 KGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDLRRFLSR 257
           KGYDGAKID+WSCGIIL+VL  GYLPFND NLM MYRKIY+G+FR PKWTSPDLRR L+R
Sbjct: 196 KGYDGAKIDIWSCGIILFVLNAGYLPFNDHNLMVMYRKIYKGEFRIPKWTSPDLRRLLTR 255

Query: 258 LLDTNPETRITVDEIHQDPWFRKGFQEEKLQFE-DYSSSELYGDVNNSKC----LNAFDL 317
           LLDTNP+TRIT++EI  DPWF++G+ +   +F  + S  +L  D  +S+     +NAFD+
Sbjct: 256 LLDTNPQTRITIEEIIHDPWFKQGYDDRMSKFHLEDSDMKLPADETDSEMGARRMNAFDI 315

Query: 318 ISFSPGFDLTGLINETEFSSGVERFLSAESPEKIIVTVEEVSKSENAAV-EKNGWGAKVE 377
           IS SPGF+L+GL  +      VERF+SA + E+++  +EE+  +EN  V +K  WG K+E
Sbjct: 316 ISGSPGFNLSGLFGDARKYDRVERFVSAWTAERVVERLEEIVSAENLTVAKKETWGMKIE 375

Query: 378 GHDGGFVAVIDIFRLTEELVVVEIKNRVREI---DQIWKDKLRPQLECLIYRSD 423
           G  G F  V++I +LT+ELV++E++ R R       +W D LRP    L++ SD
Sbjct: 376 GQKGNFAMVVEINQLTDELVMIEVRKRQRAAASGRDLWTDTLRPFFVELVHESD 429

BLAST of Tan0002627 vs. TAIR 10
Match: AT2G30360.1 (SOS3-interacting protein 4 )

HSP 1 Score: 493.0 bits (1268), Expect = 2.3e-139
Identity = 258/424 (60.85%), Postives = 320/424 (75.47%), Query Frame = 0

Query: 1   MPEFDDGNSSPELAGADGILFGKYELGKLLGCGAFAKVFHGRNVLTGQSVAIKAVNKLKV 60
           MPE +    S +   A   LFGKYELGKLLGCGAFAKVFH R+  TGQSVA+K +NK K+
Sbjct: 1   MPEIEIAAGSGDNNDA---LFGKYELGKLLGCGAFAKVFHARDRRTGQSVAVKILNKKKL 60

Query: 61  LKGGFTA-HVKREIAIMRRLRHPNVVRLFEVLATKKKIYFVMEFAKGGELFAKVSK-GRF 120
           L     A ++KREI+IMRRL HPN+V+L EV+ATK KI+F MEF KGGELF K+SK GR 
Sbjct: 61  LTNPALANNIKREISIMRRLSHPNIVKLHEVMATKSKIFFAMEFVKGGELFNKISKHGRL 120

Query: 121 SENLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSAFTDQIRPDG 180
           SE+LSRRYFQQLI+AVGYCH+RG++HRDLKPENLL+D+N +L+VSDFGLSA TDQIRPDG
Sbjct: 121 SEDLSRRYFQQLISAVGYCHARGVYHRDLKPENLLIDENGNLKVSDFGLSALTDQIRPDG 180

Query: 181 LLHTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIYR 240
           LLHTLCGTPAYVAPEIL+KKGY+GAK+DVWSCGI+L+VL  GYLPFND N+M MY+KIY+
Sbjct: 181 LLHTLCGTPAYVAPEILSKKGYEGAKVDVWSCGIVLFVLVAGYLPFNDPNVMNMYKKIYK 240

Query: 241 GDFRCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWF-RKGFQEEKLQFEDYSSSEL 300
           G++R P+W SPDL+RF+SRLLD NPETRIT+DEI +DPWF R GF++ K   ++    ++
Sbjct: 241 GEYRFPRWMSPDLKRFVSRLLDINPETRITIDEILKDPWFVRGGFKQIKFHDDEIEDQKV 300

Query: 301 YGDVNNSKCLNAFDLISFSPGFDLTGLINETEFSSG-VERFLSAESPEKIIVTVEEVSKS 360
              +   K LNAFDLIS+S G DL+GL      SSG  ERFLS +SPE +   VE  ++ 
Sbjct: 301 ESSLEAVKSLNAFDLISYSSGLDLSGLFAGCSNSSGESERFLSEKSPEMLAEEVEGFARE 360

Query: 361 ENAAVEK---NGWGAKVEGHDGGFVAVIDIFRLTEELVVVEIKNRVREID---QIWKDKL 415
           EN  ++K     +G ++EG +G F   I I RL + LVVVE + R  + D   ++W  KL
Sbjct: 361 ENLRMKKKKEEEYGFEMEGQNGKFGIGICISRLNDLLVVVEARRRGGDGDCYKEMWNGKL 420

BLAST of Tan0002627 vs. TAIR 10
Match: AT2G38490.1 (CBL-interacting protein kinase 22 )

HSP 1 Score: 454.9 bits (1169), Expect = 7.0e-128
Identity = 239/425 (56.24%), Postives = 303/425 (71.29%), Query Frame = 0

Query: 5   DDGNSSPEL----AGAD--GILFGKYELGKLLGCGAFAKVFHGRNVLT-GQSVAIKAVNK 64
           +D NSS  +     G D    LFGKY+LGKLLG GAFAKV+   ++   G+SVAIK V K
Sbjct: 27  EDSNSSESIIVNVTGDDNKSALFGKYDLGKLLGSGAFAKVYQAEDLQNGGESVAIKVVQK 86

Query: 65  LKVLKGGFTAHVKREIAIMRRLRHPNVVRLFEVLATKKKIYFVMEFAKGGELFAKVSKGR 124
            K LK G TAHVKREI++MRRLRHP++V L EVLATK KIYFVME AKGGELF++V+  R
Sbjct: 87  -KRLKDGLTAHVKREISVMRRLRHPHIVLLSEVLATKTKIYFVMELAKGGELFSRVTSNR 146

Query: 125 FSENLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSAFTDQIRPD 184
           F+E+LSR+YF+QLI+AV YCH+RG+FHRDLKPENLLLD+N DL+VSDFGLSA  +QI PD
Sbjct: 147 FTESLSRKYFRQLISAVRYCHARGVFHRDLKPENLLLDENRDLKVSDFGLSAMKEQIHPD 206

Query: 185 GLLHTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIY 244
           G+LHTLCGTPAYVAPE+L KKGYDG+K D+WSCG++L++L  GYLPF D N+M +YRKI+
Sbjct: 207 GMLHTLCGTPAYVAPELLLKKGYDGSKADIWSCGVVLFLLNAGYLPFRDPNIMGLYRKIH 266

Query: 245 RGDFRCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWFRKGFQEEK---LQFEDYSS 304
           +  ++ P WTS DLR+ L RLL+ NPE RITV+EI +DPWF  G    +   +Q +DY  
Sbjct: 267 KAQYKLPDWTSSDLRKLLRRLLEPNPELRITVEEILKDPWFNHGVDPSEIIGIQADDYDL 326

Query: 305 SELYGDVNNSKCLNAFDLISFSPGFDLTGLINETEFSSGVERFLSAESPEKIIVTVEEVS 364
            E      N K LNAFDLIS +   +L+GL          ++F+S ES   I+  VEEV+
Sbjct: 327 EE------NGKILNAFDLISSASSSNLSGLFGNFVTPDHCDQFVSDESTAVIMRKVEEVA 386

Query: 365 KSENAAVEKNGWGA-KVEGHDGGFVAVIDIFRLTEELVVVEIKNRVREIDQIWKDKLRPQ 419
           K  N  + K    A K+EG  G    V+ + RLT ELV+VE+KN+ R++  +W D LR +
Sbjct: 387 KQLNLRIAKKKERAIKLEGPHGVANVVVKVRRLTNELVMVEMKNKQRDVGLVWADALRQK 444

BLAST of Tan0002627 vs. TAIR 10
Match: AT4G18700.1 (CBL-interacting protein kinase 12 )

HSP 1 Score: 452.2 bits (1162), Expect = 4.5e-127
Identity = 233/445 (52.36%), Postives = 303/445 (68.09%), Query Frame = 0

Query: 12  ELAGADGILFGKYELGKLLGCGAFAKVFHGRNVLTGQSVAIKAVNKLKVLKGGFTAHVKR 71
           E +    ++ G+YE+GKLLG G FAKV+  RNV T +SVAIK ++K KVLKGG  AH+KR
Sbjct: 14  ERSSPQALILGRYEMGKLLGHGTFAKVYLARNVKTNESVAIKVIDKEKVLKGGLIAHIKR 73

Query: 72  EIAIMRRLRHPNVVRLFEVLATKKKIYFVMEFAKGGELFAKVSKGRFSENLSRRYFQQLI 131
           EI+I+RR+RHPN+V+LFEV+ATK KIYFVME+ +GGELF KV+KGR  E ++R+YFQQLI
Sbjct: 74  EISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFNKVAKGRLKEEVARKYFQQLI 133

Query: 132 TAVGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSAFTDQIRPDGLLHTLCGTPAYVA 191
           +AV +CH+RG++HRDLKPENLLLD+N +L+VSDFGLSA +DQIR DGL HT CGTPAYVA
Sbjct: 134 SAVTFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTPAYVA 193

Query: 192 PEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIYRGDFRCPKWTSPDL 251
           PE+LA+KGYD AK+D+WSCG+IL+VL  GYLPF+D N+MAMY+KIYRG+FRCP+W S +L
Sbjct: 194 PEVLARKGYDAAKVDIWSCGVILFVLMAGYLPFHDRNVMAMYKKIYRGEFRCPRWFSTEL 253

Query: 252 RRFLSRLLDTNPETRITVDEIHQDPWFRKGFQEEKLQFED-------------------- 311
            R LS+LL+TNPE R T  EI ++ WF+KGF+  K   ED                    
Sbjct: 254 TRLLSKLLETNPEKRFTFPEIMENSWFKKGFKHIKFYVEDDKLCNVVDDDELESDSVESD 313

Query: 312 ----YSSSEL--------YGDVNNSKCLNAFDLISFSPGFDLTGLINETEFSSGVERFLS 371
                S SE+         G +     LNAFD+ISFS GFDL+GL ++    S   RF+S
Sbjct: 314 RDSAASESEIEYLEPRRRVGGLPRPASLNAFDIISFSQGFDLSGLFDDDGEGS---RFVS 373

Query: 372 AESPEKIIVTVEEVSKSENAAVEKNGWGAKVEGH----DGGFVAVIDIFRLTEELVVVEI 418
                KII  +EE++K  +  V K      +EG      G      +IF LT  LVVVE+
Sbjct: 374 GAPVSKIISKLEEIAKVVSFTVRKKDCRVSLEGSRQGVKGPLTIAAEIFELTPSLVVVEV 433

BLAST of Tan0002627 vs. TAIR 10
Match: AT1G29230.1 (CBL-interacting protein kinase 18 )

HSP 1 Score: 447.2 bits (1149), Expect = 1.5e-125
Identity = 234/456 (51.32%), Postives = 301/456 (66.01%), Query Frame = 0

Query: 6   DGNSSPE-----LAGADGILFGKYELGKLLGCGAFAKVFHGRNVLTGQSVAIKAVNKLKV 65
           DGN SP+      +  + IL GKYELGKLLG G FAKV+  +N+ +G  VAIK ++K K+
Sbjct: 51  DGNISPQSPRSPRSPRNNILMGKYELGKLLGHGTFAKVYLAQNIKSGDKVAIKVIDKEKI 110

Query: 66  LKGGFTAHVKREIAIMRRLRHPNVVRLFEVLATKKKIYFVMEFAKGGELFAKVSKGRFSE 125
           +K G  AH+KREI+I+RR+RHP +V LFEV+ATK KIYFVME+  GGELF  V+KGR  E
Sbjct: 111 MKSGLVAHIKREISILRRVRHPYIVHLFEVMATKSKIYFVMEYVGGGELFNTVAKGRLPE 170

Query: 126 NLSRRYFQQLITAVGYCHSRGIFHRDLKPENLLLDDNFDLRVSDFGLSAFTDQIRPDGLL 185
             +RRYFQQLI++V +CH RG++HRDLKPENLLLD+  +L+VSDFGLSA  +Q+R DGL 
Sbjct: 171 ETARRYFQQLISSVSFCHGRGVYHRDLKPENLLLDNKGNLKVSDFGLSAVAEQLRQDGLC 230

Query: 186 HTLCGTPAYVAPEILAKKGYDGAKIDVWSCGIILYVLTTGYLPFNDSNLMAMYRKIYRGD 245
           HT CGTPAY+APE+L +KGYD AK DVWSCG+IL+VL  G++PF D N+M MY+KIY+G+
Sbjct: 231 HTFCGTPAYIAPEVLTRKGYDAAKADVWSCGVILFVLMAGHIPFYDKNIMVMYKKIYKGE 290

Query: 246 FRCPKWTSPDLRRFLSRLLDTNPETRITVDEIHQDPWFRKGFQEEKLQFED--------- 305
           FRCP+W S DL R L+RLLDTNP+TRIT+ EI ++ WF+KGF+  K   ED         
Sbjct: 291 FRCPRWFSSDLVRLLTRLLDTNPDTRITIPEIMKNRWFKKGFKHVKFYIEDDKLCREDED 350

Query: 306 -------------YSSSELYGDVN--------NSKCLNAFDLISFSPGFDLTGLINETEF 365
                         S S+   DV             LNAFD+ISFS GFDL+GL  E E 
Sbjct: 351 EEEEASSSGRSSTVSESDAEFDVKRMGIGSMPRPSSLNAFDIISFSSGFDLSGLFEE-EG 410

Query: 366 SSGVERFLSAESPEKIIVTVEEVSKSENAAVEKNGWGAKV----EGHDGGFVAVIDIFRL 420
             G  RF+S     KII  +EE++K  +  V K  W  ++    EG  G      +IF L
Sbjct: 411 GEGT-RFVSGAPVSKIISKLEEIAKIVSFTVRKKEWSLRLEGCREGAKGPLTIAAEIFEL 470

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LZW43.8e-15564.73CBL-interacting serine/threonine-protein kinase 14 OS=Arabidopsis thaliana OX=37... [more]
O229323.3e-13860.85CBL-interacting serine/threonine-protein kinase 11 OS=Arabidopsis thaliana OX=37... [more]
O809029.9e-12756.24CBL-interacting serine/threonine-protein kinase 22 OS=Arabidopsis thaliana OX=37... [more]
Q9SN436.4e-12652.36CBL-interacting serine/threonine-protein kinase 12 OS=Arabidopsis thaliana OX=37... [more]
Q68Y491.4e-12549.17CBL-interacting protein kinase 19 OS=Oryza sativa subsp. japonica OX=39947 GN=CI... [more]
Match NameE-valueIdentityDescription
XP_022949666.17.1e-22392.07CBL-interacting serine/threonine-protein kinase 14-like [Cucurbita moschata][more]
XP_023545198.11.2e-22292.07CBL-interacting serine/threonine-protein kinase 14-like [Cucurbita pepo subsp. p... [more]
KAG7033738.11.6e-22292.29CBL-interacting serine/threonine-protein kinase 14, partial [Cucurbita argyrospe... [more]
XP_022924879.14.6e-22291.71CBL-interacting serine/threonine-protein kinase 14-like [Cucurbita moschata][more]
XP_023517341.11.7e-22191.47CBL-interacting serine/threonine-protein kinase 14-like [Cucurbita pepo subsp. p... [more]
Match NameE-valueIdentityDescription
A0A6J1GCM33.4e-22392.07Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1EAG32.2e-22291.71CBL-interacting serine/threonine-protein kinase 14-like OS=Cucurbita moschata OX... [more]
A0A6J1ITF48.4e-22291.61Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1HRC52.1e-22091.00CBL-interacting serine/threonine-protein kinase 14-like OS=Cucurbita maxima OX=3... [more]
A0A0A0L2V29.0e-21689.12Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_4... [more]
Match NameE-valueIdentityDescription
AT5G01820.12.7e-15664.73serine/threonine protein kinase 1 [more]
AT2G30360.12.3e-13960.85SOS3-interacting protein 4 [more]
AT2G38490.17.0e-12856.24CBL-interacting protein kinase 22 [more]
AT4G18700.14.5e-12752.36CBL-interacting protein kinase 12 [more]
AT1G29230.11.5e-12551.32CBL-interacting protein kinase 18 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 24..278
e-value: 1.9E-106
score: 369.6
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 24..278
e-value: 1.5E-70
score: 237.6
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 24..278
score: 51.240711
IPR004041NAF domainPFAMPF03822NAFcoord: 305..363
e-value: 8.8E-17
score: 61.0
NoneNo IPR availableGENE3D3.30.310.80Kinase associated domain 1, KA1coord: 303..420
e-value: 6.9E-30
score: 105.5
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 104..284
e-value: 5.1E-59
score: 200.8
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 16..103
e-value: 7.7E-28
score: 98.5
NoneNo IPR availablePIRSRPIRSR037921-2PIRSR037921-2coord: 28..231
e-value: 1.3E-11
score: 42.2
NoneNo IPR availablePIRSRPIRSR000620-2PIRSR000620-2coord: 25..272
e-value: 5.2E-13
score: 46.5
NoneNo IPR availablePANTHERPTHR43895FAMILY NOT NAMEDcoord: 12..420
NoneNo IPR availablePANTHERPTHR43895:SF22CBL-INTERACTING SERINE/THREONINE-PROTEIN KINASE 14coord: 12..420
NoneNo IPR availableCDDcd12195CIPK_Ccoord: 308..414
e-value: 2.12654E-32
score: 116.903
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 30..53
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 142..154
IPR018451NAF/FISL domainPROSITEPS50816NAFcoord: 302..326
score: 11.230983
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 20..290

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0002627.1Tan0002627.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0007165 signal transduction
molecular_function GO:0005524 ATP binding
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity