Tan0001134 (gene) Snake gourd v1

Overview
NameTan0001134
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionalkane hydroxylase MAH1-like
LocationLG03: 70525475 .. 70527014 (-)
RNA-Seq ExpressionTan0001134
SyntenyTan0001134
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGTCCATTGCCATTCTCTCCATCCTTCTCTTTTCATTACTCTTCATTATTTTCTCTGTTTTTGTAAAATGGAACCCTTATGGGCTTCCTTGGAGTTGGCCTCTTTTCGGAATGATCCCTACAATTGTCTTCCATATCCACCGAATTCATGACCGTGTAACTGAAATCTTAAAACAAGCTGGTTCCACTTTCTTCTTCAATGGCGTTTGGTTTGCTGATATTGACTTCTTGCTCACAGCCGATCCTTCAAATATACACCATATAATGAGTGTCAATTTTGAGCGATATCCAAAAGGGCCTGATTTCAAGTATATTTTTGACGTTTTGGGAGATGGGATTTTCAACTCCGACTCTGATTCATGGAAGAATCAACGCAAAACCGCTCATTCCTTGGTCCGCCATGAGAGTTTTCTTCAATTCTTGGAGAAAATTGTGATGAAGAAGGTGAATCAAGGGGCTGATTCCAGTGCTTGAAAGTGTTTGTGAAAATGGGTCGGTATTGGATTTGCAAGATTTGTTTCAGAGATTTTCGTTTGATTCCACTTGTATGTTGGTCACCGGCTTCGACCTCCATAGTTTATCTCTCGAATTTCCTCAAGTTCCCTTTTCCAAAGCCATGGATGATGTGGAGGAAGTGATTTTTCTTCGACACTTTTTTCCTAAAAAAATTTGGGAATTACAAAAGAAACTCCAATTTGGTGAAGCCAAGAGGCTGAAACAAGCTTGGGAAATCATAGATCAAACCATCGAAAAATTGCTAGCCTCAAAAAGAGAGAGCTTGAAAAAGCTGAAAGAAGAGCCAGCTGAAGTAGGAGTAGGAGGAGTTGATTTGATCACATCATATATTACAAACAAATTAGAGGATGAAATTAGCAACAACAACGACAAGTTCTTGAGAGACACTATTTTGAATTTCATGATTGCTGGTCGAGACACTCTCAGCTCAGCACTCTCTTGGTTTTTCTTTTGTCTTTCAAACAATCCAATGGTGGAAGCAAAGATAAGAGAAGAGCTCAAAGGAATCATCAATATCCCAACAAACGAAGCTCATGATGAATGGAGAATATTTTCAATTGAAGAGCTGAAGAATCTAGTTTACTTTCATGGTGCTTTATGTGAAACACTTAGACTTTATCCTCCAGTTCCATTTCAACACAAAGTCCCTTCGCAACCTGACATTCTTCCATCAGGCCATCATGTCAATCCCAAAACAAAGATTCTGTTTTCGTTATATGCATTAGGAAGAACAAGTGATGTTTGGGGAAAAGATTGCATGGAATTCAAGCCAGAGAGATGGATTTCAGAAAATGGAAGGATCAAGCATGTGCCATCTTACAAATTCTTGGCCTTTAACGCAGGGCCAAGGACTTGTTTAGGAAAAGAAGTGGCTTTTCTTCAGATGAAAATAGTTGCGGCTGCCATCATTCACAACTACAATCTTCAACACATTGGCCGCCATAAGGTTGTTCCTAGTGCTTCTATTATTCTTCACATGAAGCATGGATTCGAAGTCATGGTTACTAAGTTTACTTCATAA

mRNA sequence

ATGGCGTCCATTGCCATTCTCTCCATCCTTCTCTTTTCATTACTCTTCATTATTTTCTCTGTTTTTGTAAAATGGAACCCTTATGGGCTTCCTTGGAGTTGGCCTCTTTTCGGAATGATCCCTACAATTGTCTTCCATATCCACCGAATTCATGACCGTGTAACTGAAATCTTAAAACAAGCTGGTTCCACTTTCTTCTTCAATGGCGTTTGGTTTGCTGATATTGACTTCTTGCTCACAGCCGATCCTTCAAATATACACCATATAATGAGTGTCAATTTTGAGCGATATCCAAAAGGGCCTGATTTCAAGTATATTTTTGACGTTTTGGGAGATGGGATTTTCAACTCCGACTCTGATTCATGGAAGAATCAACGCAAAACCGCTCATTCCTTGGGGCTGATTCCAGTGCTTGAAAGTGTTTGTGAAAATGGGTCGGTATTGGATTTGCAAGATTTGTTTCAGAGATTTTCGTTTGATTCCACTTGTATGTTGGTCACCGGCTTCGACCTCCATAGTTTATCTCTCGAATTTCCTCAAGTTCCCTTTTCCAAAGCCATGGATGATGTGGAGGAAGTGATTTTTCTTCGACACTTTTTTCCTAAAAAAATTTGGGAATTACAAAAGAAACTCCAATTTGGTGAAGCCAAGAGGCTGAAACAAGCTTGGGAAATCATAGATCAAACCATCGAAAAATTGCTAGCCTCAAAAAGAGAGAGCTTGAAAAAGCTGAAAGAAGAGCCAGCTGAAGTAGGAGTAGGAGGAGTTGATTTGATCACATCATATATTACAAACAAATTAGAGGATGAAATTAGCAACAACAACGACAAGTTCTTGAGAGACACTATTTTGAATTTCATGATTGCTGGTCGAGACACTCTCAGCTCAGCACTCTCTTGGTTTTTCTTTTGTCTTTCAAACAATCCAATGGTGGAAGCAAAGATAAGAGAAGAGCTCAAAGGAATCATCAATATCCCAACAAACGAAGCTCATGATGAATGGAGAATATTTTCAATTGAAGAGCTGAAGAATCTAGTTTACTTTCATGGTGCTTTATGTGAAACACTTAGACTTTATCCTCCAGTTCCATTTCAACACAAAGTCCCTTCGCAACCTGACATTCTTCCATCAGGCCATCATGTCAATCCCAAAACAAAGATTCTGTTTTCGTTATATGCATTAGGAAGAACAAGTGATGTTTGGGGAAAAGATTGCATGGAATTCAAGCCAGAGAGATGGATTTCAGAAAATGGAAGGATCAAGCATGTGCCATCTTACAAATTCTTGGCCTTTAACGCAGGGCCAAGGACTTGTTTAGGAAAAGAAGTGGCTTTTCTTCAGATGAAAATAGTTGCGGCTGCCATCATTCACAACTACAATCTTCAACACATTGGCCGCCATAAGGTTGTTCCTAGTGCTTCTATTATTCTTCACATGAAGCATGGATTCGAAGTCATGGTTACTAAGTTTACTTCATAA

Coding sequence (CDS)

ATGGCGTCCATTGCCATTCTCTCCATCCTTCTCTTTTCATTACTCTTCATTATTTTCTCTGTTTTTGTAAAATGGAACCCTTATGGGCTTCCTTGGAGTTGGCCTCTTTTCGGAATGATCCCTACAATTGTCTTCCATATCCACCGAATTCATGACCGTGTAACTGAAATCTTAAAACAAGCTGGTTCCACTTTCTTCTTCAATGGCGTTTGGTTTGCTGATATTGACTTCTTGCTCACAGCCGATCCTTCAAATATACACCATATAATGAGTGTCAATTTTGAGCGATATCCAAAAGGGCCTGATTTCAAGTATATTTTTGACGTTTTGGGAGATGGGATTTTCAACTCCGACTCTGATTCATGGAAGAATCAACGCAAAACCGCTCATTCCTTGGGGCTGATTCCAGTGCTTGAAAGTGTTTGTGAAAATGGGTCGGTATTGGATTTGCAAGATTTGTTTCAGAGATTTTCGTTTGATTCCACTTGTATGTTGGTCACCGGCTTCGACCTCCATAGTTTATCTCTCGAATTTCCTCAAGTTCCCTTTTCCAAAGCCATGGATGATGTGGAGGAAGTGATTTTTCTTCGACACTTTTTTCCTAAAAAAATTTGGGAATTACAAAAGAAACTCCAATTTGGTGAAGCCAAGAGGCTGAAACAAGCTTGGGAAATCATAGATCAAACCATCGAAAAATTGCTAGCCTCAAAAAGAGAGAGCTTGAAAAAGCTGAAAGAAGAGCCAGCTGAAGTAGGAGTAGGAGGAGTTGATTTGATCACATCATATATTACAAACAAATTAGAGGATGAAATTAGCAACAACAACGACAAGTTCTTGAGAGACACTATTTTGAATTTCATGATTGCTGGTCGAGACACTCTCAGCTCAGCACTCTCTTGGTTTTTCTTTTGTCTTTCAAACAATCCAATGGTGGAAGCAAAGATAAGAGAAGAGCTCAAAGGAATCATCAATATCCCAACAAACGAAGCTCATGATGAATGGAGAATATTTTCAATTGAAGAGCTGAAGAATCTAGTTTACTTTCATGGTGCTTTATGTGAAACACTTAGACTTTATCCTCCAGTTCCATTTCAACACAAAGTCCCTTCGCAACCTGACATTCTTCCATCAGGCCATCATGTCAATCCCAAAACAAAGATTCTGTTTTCGTTATATGCATTAGGAAGAACAAGTGATGTTTGGGGAAAAGATTGCATGGAATTCAAGCCAGAGAGATGGATTTCAGAAAATGGAAGGATCAAGCATGTGCCATCTTACAAATTCTTGGCCTTTAACGCAGGGCCAAGGACTTGTTTAGGAAAAGAAGTGGCTTTTCTTCAGATGAAAATAGTTGCGGCTGCCATCATTCACAACTACAATCTTCAACACATTGGCCGCCATAAGGTTGTTCCTAGTGCTTCTATTATTCTTCACATGAAGCATGGATTCGAAGTCATGGTTACTAAGTTTACTTCATAA

Protein sequence

MASIAILSILLFSLLFIIFSVFVKWNPYGLPWSWPLFGMIPTIVFHIHRIHDRVTEILKQAGSTFFFNGVWFADIDFLLTADPSNIHHIMSVNFERYPKGPDFKYIFDVLGDGIFNSDSDSWKNQRKTAHSLGLIPVLESVCENGSVLDLQDLFQRFSFDSTCMLVTGFDLHSLSLEFPQVPFSKAMDDVEEVIFLRHFFPKKIWELQKKLQFGEAKRLKQAWEIIDQTIEKLLASKRESLKKLKEEPAEVGVGGVDLITSYITNKLEDEISNNNDKFLRDTILNFMIAGRDTLSSALSWFFFCLSNNPMVEAKIREELKGIINIPTNEAHDEWRIFSIEELKNLVYFHGALCETLRLYPPVPFQHKVPSQPDILPSGHHVNPKTKILFSLYALGRTSDVWGKDCMEFKPERWISENGRIKHVPSYKFLAFNAGPRTCLGKEVAFLQMKIVAAAIIHNYNLQHIGRHKVVPSASIILHMKHGFEVMVTKFTS
Homology
BLAST of Tan0001134 vs. ExPASy Swiss-Prot
Match: Q9FVS9 (Alkane hydroxylase MAH1 OS=Arabidopsis thaliana OX=3702 GN=CYP96A15 PE=2 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 6.5e-122
Identity = 226/515 (43.88%), Postives = 320/515 (62.14%), Query Frame = 0

Query: 1   MASIAILSILLFSLLFIIFSVFVKWNPYGLP--WSWPLFGMIPTIVFHIHRIHDRVTEIL 60
           MA +      +F L+ +    F++  P G P   +WP   M+P ++  I RI+D   E+L
Sbjct: 1   MAMLGFYVTFIFFLVCLFTYFFLQKKPQGQPILKNWPFLRMLPGMLHQIPRIYDWTVEVL 60

Query: 61  KQAGSTFFFNGVWFADIDFLLTADPSNIHHIMSVNFERYPKGPDFKYIFDVLGDGIFNSD 120
           +    TF+F G W +  D L TADP NIHHI+S NF  YPKGP+FK IFDVLG+GI   D
Sbjct: 61  EATNLTFYFKGPWLSGTDMLFTADPRNIHHILSSNFGNYPKGPEFKKIFDVLGEGILTVD 120

Query: 121 SDSWKNQRKTAHSL--------------------GLIPVLESVCENGSVLDLQDLFQRFS 180
            + W+  RK+ H+L                    GL+P L++  +   +++LQD+FQRF 
Sbjct: 121 FELWEEMRKSNHALFHNQDFIELSVSSNKSKLKEGLVPFLDNAAQKNIIIELQDVFQRFM 180

Query: 181 FDSTCMLVTGFDLHSLSLEFPQVPFSKAMDDVEEVIFLRHFFPKKIWELQKKLQFGEAKR 240
           FD++ +L+TG+D  SLS+E  +V F +A D  EE I+ RHF P  +W LQ  +  G  ++
Sbjct: 181 FDTSSILMTGYDPMSLSIEMLEVEFGEAADIGEEAIYYRHFKPVILWRLQNWIGIGLERK 240

Query: 241 LKQAWEIIDQTIEKLLASKR-ESLKKLKEEPAEVGVGGVDLITSYI---TNKLEDEISNN 300
           ++ A   +++   K+++S+R E + + K EP        D +T Y+   T+K +  +  N
Sbjct: 241 MRTALATVNRMFAKIISSRRKEEISRAKTEPY-----SKDALTYYMNVDTSKYK-LLKPN 300

Query: 301 NDKFLRDTILNFMIAGRDTLSSALSWFFFCLSNNPMVEAKIREELKGIINIPTNEAHDEW 360
            DKF+RD I + ++AGRDT SS L+WFF+ LS +P V AK+R E+               
Sbjct: 301 KDKFIRDVIFSLVLAGRDTTSSVLTWFFWLLSKHPQVMAKLRHEIN-------------- 360

Query: 361 RIFSIEELKNLVYFHGALCETLRLYPPVPFQHKVPSQPDILPSGHHVNPKTKILFSLYAL 420
             F  E+L+ LVY H AL E++RLYPP+PF HK P++PD+LPSGH V+  +KI+  +YAL
Sbjct: 361 TKFDNEDLEKLVYLHAALSESMRLYPPLPFNHKSPAKPDVLPSGHKVDANSKIVICIYAL 420

Query: 421 GRTSDVWGKDCMEFKPERWISENGRIKHVPSYKFLAFNAGPRTCLGKEVAFLQMKIVAAA 480
           GR   VWG+D ++FKPERWIS+NG ++H PSYKF+AFN+GPRTCLGK +A LQMK+VA  
Sbjct: 421 GRMRSVWGEDALDFKPERWISDNGGLRHEPSYKFMAFNSGPRTCLGKNLALLQMKMVALE 480

Query: 481 IIHNYNLQHIGRHKVVPSASIILHMKHGFEVMVTK 490
           II NY+ + I  HKV P  SI+L MKHG +V VTK
Sbjct: 481 IIRNYDFKVIEGHKVEPIPSILLRMKHGLKVTVTK 495

BLAST of Tan0001134 vs. ExPASy Swiss-Prot
Match: A0A140IL90 (Noroxomaritidine synthase OS=Narcissus aff. pseudonarcissus MK-2014 OX=1540222 GN=Cyp96T1 PE=1 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 5.5e-113
Identity = 212/508 (41.73%), Postives = 305/508 (60.04%), Query Frame = 0

Query: 4   IAILSILLFSLLFIIFSVFVKWNPYGLPWSWPLFGMIPTIVFHIHRIHDRVTEILKQAGS 63
           IAI   L+FSLL    S     +   LP++WP+FGM+P I+ + ++ +D  T  L++ G 
Sbjct: 20  IAIACFLIFSLLLSARSS----SEDSLPYNWPIFGMLPAIISN-NQFNDFTTARLRKMGW 79

Query: 64  TFFFNGVWFADIDFLLTADPSNIHHIMSVNFERYPKGPDFKYIFDVLGDGIFNSDSDSWK 123
           TF F G W  D+D++ T DPSNI+H+ + NFE YPKG +   +FD+ G+ IFN+D D W 
Sbjct: 80  TFIFKGPWLLDMDYIFTCDPSNINHMFNDNFENYPKG-ELGKVFDIFGNNIFNADGDLWH 139

Query: 124 NQRKTAHSL--------------------GLIPVLESVCENGSVLDLQDLFQRFSFDSTC 183
           + RK A ++                     LIP+L+S       +DLQD+F RF+FD++C
Sbjct: 140 DHRKMAQTILWDGNYRTMQATFIRNKMDNALIPILDSAASKRKPVDLQDVFFRFTFDTSC 199

Query: 184 MLVTGFDLHSLSLEFPQVPFSKAMDDVEEVIFLRHFFPKKIWELQKKLQFGEAKRLKQAW 243
             V   D  SL++EFP VPFSKA D   +    RH  P+ IW+L++    G  + L  AW
Sbjct: 200 FSVLAADPESLTMEFPAVPFSKAADQALDAALTRHITPRLIWKLKRFFNVGSERTLAVAW 259

Query: 244 EIIDQTIEKLLASKRESLKKLKEEPAEVG-VGGVDLITSYITNKLEDEISNNNDKFLRDT 303
           ++ID  I        + + +LK +   VG +   D ++ Y+     D  + ++DKFLRD 
Sbjct: 260 KVIDSYI-------YDKIAELKAKRKLVGKINSYDAVSFYM-----DNFNIHDDKFLRDN 319

Query: 304 ILNFMIAGRDTLSSALSWFFFCLSNNPMVEAKIREELKGIINIPT-NEAHDEWRIFSIEE 363
              +++A R+T S  ++W F+ L  NP VE KI  ELK I++  +  + +D + +F    
Sbjct: 320 AFTYLLAQRNTQSLTMTWLFYALFENPKVELKILSELKSIVDESSERKFNDGFALFDSNM 379

Query: 364 LKNLVYFHGALCETLRLYPPVPFQHKVPSQPDILPSGHHVNPKTKILFSLYALGRTSDVW 423
           +++ +Y H  LCE LR+YPPVPF+ K   + D+LPSGH V    KILFS YA+ R   +W
Sbjct: 380 IQSAIYLHATLCEALRIYPPVPFEIKDAHKADVLPSGHKVRAGEKILFSPYAMARMKGIW 439

Query: 424 GKDCMEFKPERWISENGRIKHVPSYKFLAFNAGPRTCLGKEVAFLQMKIVAAAIIHNYNL 483
           G DC+EFKPERWI+ NG +KH P+YKF AF+AGPR CLGKE++F QMK+V A II+N++L
Sbjct: 440 GDDCLEFKPERWITGNGTLKHEPAYKFFAFSAGPRICLGKELSFTQMKMVVATIIYNFHL 499

Query: 484 QHIGRHKVVPSASIILHMKHGFEVMVTK 490
           Q +  H V  S SI++ MKHG  V V K
Sbjct: 500 QMVKGHVVEQSNSILMDMKHGLMVQVRK 509

BLAST of Tan0001134 vs. ExPASy Swiss-Prot
Match: A0A140IL92 (Noroxomaritidine synthase 3 OS=Narcissus aff. pseudonarcissus MK-2014 OX=1540222 GN=Cyp96T3 PE=2 SV=1)

HSP 1 Score: 408.7 bits (1049), Expect = 9.5e-113
Identity = 212/508 (41.73%), Postives = 303/508 (59.65%), Query Frame = 0

Query: 4   IAILSILLFSLLFIIFSVFVKWNPYGLPWSWPLFGMIPTIVFHIHRIHDRVTEILKQAGS 63
           IAI   L+FSLL    S     +   LP++WP+FGM+P I+ +  + +D  T  L++ G 
Sbjct: 20  IAIACFLIFSLLLSARSS----SEDSLPYNWPIFGMLPAIISN-SQFNDFTTARLRKMGW 79

Query: 64  TFFFNGVWFADIDFLLTADPSNIHHIMSVNFERYPKGPDFKYIFDVLGDGIFNSDSDSWK 123
           TF F G W  D+D++ T DPSNI+H+ + NFE YPKG +   +FD+ G+ IFN+D D W 
Sbjct: 80  TFIFKGPWLLDMDYIFTCDPSNINHMFNDNFENYPKG-ELGKVFDIFGNNIFNADGDLWH 139

Query: 124 NQRKTAHSL--------------------GLIPVLESVCENGSVLDLQDLFQRFSFDSTC 183
           + RK A ++                     LIP+L+S       +DLQD+F RF+FD++C
Sbjct: 140 DHRKMAQTILWDGNYRTMQATFIRNKMDNALIPILDSAASKRKPVDLQDVFFRFTFDTSC 199

Query: 184 MLVTGFDLHSLSLEFPQVPFSKAMDDVEEVIFLRHFFPKKIWELQKKLQFGEAKRLKQAW 243
             V   D  SL++EFP VPFSKA D   +    RH  P+ IW+L++    G  + L  AW
Sbjct: 200 FSVLAADPESLTMEFPAVPFSKAADQALDAALTRHITPRLIWKLKRFFNVGSERTLAVAW 259

Query: 244 EIIDQTIEKLLASKRESLKKLKEEPAEVG-VGGVDLITSYITNKLEDEISNNNDKFLRDT 303
           ++ID  I        + + +LK +   VG +   D ++ Y+     D  + ++DKFLRD 
Sbjct: 260 KVIDSYI-------YDKIAELKAKRKLVGKINSYDAVSFYM-----DNFNIHDDKFLRDN 319

Query: 304 ILNFMIAGRDTLSSALSWFFFCLSNNPMVEAKIREELKGIINIPTNEA-HDEWRIFSIEE 363
              +++A R+T S  ++W F+ L  NP VE KI  ELK I++  +    +D + +F    
Sbjct: 320 AFTYLLAQRNTQSLTMAWLFYALFENPKVELKILSELKSIVDESSERRFNDGFALFDSNM 379

Query: 364 LKNLVYFHGALCETLRLYPPVPFQHKVPSQPDILPSGHHVNPKTKILFSLYALGRTSDVW 423
           +++ +Y H  LCE LR+YPPVPF+ K   + D+LPSGH V    KILFS YA+ R   +W
Sbjct: 380 IQSAIYLHATLCEALRIYPPVPFEIKDAHKADVLPSGHKVRAGEKILFSPYAMARMKGIW 439

Query: 424 GKDCMEFKPERWISENGRIKHVPSYKFLAFNAGPRTCLGKEVAFLQMKIVAAAIIHNYNL 483
           G DC+EFKPERWI+ NG +KH P+YKF AF+AGPR CLGKE++F QMK+V A II+N++L
Sbjct: 440 GDDCLEFKPERWITGNGTLKHEPAYKFFAFSAGPRICLGKELSFTQMKMVVATIIYNFHL 499

Query: 484 QHIGRHKVVPSASIILHMKHGFEVMVTK 490
           Q +  H V  S SI++ MKHG  V V K
Sbjct: 500 QMVKGHVVEQSNSILMDMKHGLMVQVRK 509

BLAST of Tan0001134 vs. ExPASy Swiss-Prot
Match: A0A140IL91 (Noroxomaritidine synthase 2 OS=Narcissus aff. pseudonarcissus MK-2014 OX=1540222 GN=Cyp96T2 PE=2 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 2.1e-112
Identity = 212/508 (41.73%), Postives = 305/508 (60.04%), Query Frame = 0

Query: 4   IAILSILLFSLLFIIFSVFVKWNPYGLPWSWPLFGMIPTIVFHIHRIHDRVTEILKQAGS 63
           IAI   L+FSLL    S     +   LP++WP+FGM+P I+ + ++ +D  T  L++ G 
Sbjct: 20  IAIACFLIFSLLLSARSS----SEDSLPYNWPIFGMLPAIISN-NQFNDFTTARLRKMGW 79

Query: 64  TFFFNGVWFADIDFLLTADPSNIHHIMSVNFERYPKGPDFKYIFDVLGDGIFNSDSDSWK 123
           TF F G W  D+D++ T DPSNI+H+ + NFE YPKG +   +FD+ G+ IFN+D D W 
Sbjct: 80  TFIFKGPWLLDMDYIFTCDPSNINHMFNDNFENYPKG-ELGKVFDIFGNNIFNADGDLWH 139

Query: 124 NQRKTAHSL--------------------GLIPVLESVCENGSVLDLQDLFQRFSFDSTC 183
           + RK A ++                     LIP+L+S       +DLQD+  RF+FD++C
Sbjct: 140 DHRKMAQTILWDGNYRTMQATFIRNKMDNALIPILDSAACKRKPVDLQDVLLRFTFDTSC 199

Query: 184 MLVTGFDLHSLSLEFPQVPFSKAMDDVEEVIFLRHFFPKKIWELQKKLQFGEAKRLKQAW 243
             V   D  SL++EFP VPFSKA D   +    RH  P+ IW+L++    G  + L  AW
Sbjct: 200 FSVLAADPESLTMEFPPVPFSKAADQALDAALTRHITPRLIWKLKRFFNVGSERTLAVAW 259

Query: 244 EIIDQTIEKLLASKRESLKKLKEEPAEVG-VGGVDLITSYITNKLEDEISNNNDKFLRDT 303
           ++ID  I        + + +LK +   VG +   D ++ Y+     D  + ++DKFLRD 
Sbjct: 260 KVIDSYI-------YDKIAELKAKRKLVGKINSYDAVSFYM-----DNFNIHDDKFLRDN 319

Query: 304 ILNFMIAGRDTLSSALSWFFFCLSNNPMVEAKIREELKGIINIPT-NEAHDEWRIFSIEE 363
              +++A R+T S  ++W F+ L  NP VE KI  ELK I++  +  + +D + +F    
Sbjct: 320 AFTYLLAQRNTQSLTMTWLFYALFENPKVELKILSELKSIVDESSERKFNDGFTLFDSNM 379

Query: 364 LKNLVYFHGALCETLRLYPPVPFQHKVPSQPDILPSGHHVNPKTKILFSLYALGRTSDVW 423
           +++ +Y H ALCE LR+YPPVPF+ K   + D+LPSGH V    KILFS YA+ R   +W
Sbjct: 380 IQSAIYLHAALCEALRIYPPVPFEIKDAHKADVLPSGHKVRAGEKILFSPYAMARMKGIW 439

Query: 424 GKDCMEFKPERWISENGRIKHVPSYKFLAFNAGPRTCLGKEVAFLQMKIVAAAIIHNYNL 483
           G DC+EFKPERWI+ NG +KH P+YKF AF+AGPR CLGKE++F QMK+V A II+N++L
Sbjct: 440 GDDCLEFKPERWITGNGTLKHEPAYKFFAFSAGPRICLGKELSFTQMKMVVATIIYNFHL 499

Query: 484 QHIGRHKVVPSASIILHMKHGFEVMVTK 490
           Q +  H V  S SI++ MKHG  V V K
Sbjct: 500 QMVKGHVVEQSNSILMDMKHGLMVQVRK 509

BLAST of Tan0001134 vs. ExPASy Swiss-Prot
Match: A0A2H5AJ00 (Noroxomaritidine synthase 1 OS=Narcissus pseudonarcissus OX=39639 GN=Cyp96T1 PE=2 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 3.6e-112
Identity = 211/508 (41.54%), Postives = 307/508 (60.43%), Query Frame = 0

Query: 4   IAILSILLFSLLFIIFSVFVKWNPYGLPWSWPLFGMIPTIVFHIHRIHDRVTEILKQAGS 63
           IAI  +++FSLL    S     +  GLP++WP+FGM+P I+ + ++ +D  T  L++ G 
Sbjct: 20  IAIACLVVFSLLRSARSS----SKGGLPYNWPIFGMLPAIISN-NQFNDFTTARLRKMGW 79

Query: 64  TFFFNGVWFADIDFLLTADPSNIHHIMSVNFERYPKGPDFKYIFDVLGDGIFNSDSDSWK 123
           TF F G W  D+D++ T DPSNI+H+ + NFE YPKG +   +FD+ G+ IFN+D D W 
Sbjct: 80  TFIFKGPWLLDMDYIFTCDPSNINHMFNDNFENYPKG-ELGKVFDIFGNNIFNADGDLWH 139

Query: 124 NQRKTAHSL--------------------GLIPVLESVCENGSVLDLQDLFQRFSFDSTC 183
           + RK A ++                     LIP+L+S     + +DLQD+  RF+FD++C
Sbjct: 140 DHRKMAQTILWDGNYRTMQATFIRNKMDNALIPILDSAACKRNPVDLQDVLLRFTFDTSC 199

Query: 184 MLVTGFDLHSLSLEFPQVPFSKAMDDVEEVIFLRHFFPKKIWELQKKLQFGEAKRLKQAW 243
             V   D  SL++EFP VPFSKA D   +    RH  P+ IW+L++    G  + L  AW
Sbjct: 200 FSVLAADPESLTMEFPPVPFSKAADQALDAALTRHITPRLIWKLKRFFNVGSERTLAVAW 259

Query: 244 EIIDQTIEKLLASKRESLKKLKEEPAEVG-VGGVDLITSYITNKLEDEISNNNDKFLRDT 303
           ++ID  I        + + +LK +   VG +   D ++ Y+     D  + ++DKFLRD 
Sbjct: 260 KVIDSYI-------YDKIAELKAKRKLVGKINSYDAVSFYM-----DNFNIHDDKFLRDN 319

Query: 304 ILNFMIAGRDTLSSALSWFFFCLSNNPMVEAKIREELKGIINIPT-NEAHDEWRIFSIEE 363
              +++A R+T S  ++W F+ L  NP VE KI  ELK I++  +  + +D + +F    
Sbjct: 320 AFTYLLAQRNTQSLTMTWLFYALFENPKVELKILSELKSIVDESSERKFNDGFALFDSNM 379

Query: 364 LKNLVYFHGALCETLRLYPPVPFQHKVPSQPDILPSGHHVNPKTKILFSLYALGRTSDVW 423
           +++ +Y H  LCE LR+YPPVPF+ K   + D+LPSGH V    KILFS YA+ R   +W
Sbjct: 380 IQSAIYLHATLCEALRIYPPVPFEIKDAHKADVLPSGHKVRAGEKILFSPYAMARMKGIW 439

Query: 424 GKDCMEFKPERWISENGRIKHVPSYKFLAFNAGPRTCLGKEVAFLQMKIVAAAIIHNYNL 483
           G DC+EFKPERWI+ NG +KH P+YKF AF+AGPR CLGKE++F QMK+V A II+N++L
Sbjct: 440 GDDCLEFKPERWITGNGTLKHEPAYKFFAFSAGPRICLGKELSFTQMKMVVATIIYNFHL 499

Query: 484 QHIGRHKVVPSASIILHMKHGFEVMVTK 490
           Q +  H V  S SI++ MKHG  V V K
Sbjct: 500 QMVKGHVVEQSNSILMDMKHGLMVQVRK 509

BLAST of Tan0001134 vs. NCBI nr
Match: XP_038882620.1 (alkane hydroxylase MAH1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 758.1 bits (1956), Expect = 4.8e-215
Identity = 382/514 (74.32%), Postives = 424/514 (82.49%), Query Frame = 0

Query: 1   MASI---AILSILLFSLLFIIFSVFVKWNPYG-LPWSWPLFGMIPTIVFHIHRIHDRVTE 60
           MASI   AIL  L    LFIIFS   +WN +G +PW+WP+ GM PT+V HI+R HDRVTE
Sbjct: 1   MASIDFSAILFALFIPFLFIIFSRISRWNFHGVVPWNWPIVGMTPTVVAHINRAHDRVTE 60

Query: 61  ILKQAGSTFFFNGVWFADIDFLLTADPSNIHHIMSVNFERYPKGPDFKYIFDVLGDGIFN 120
           +L+ AGSTFFF GVWF+ +DFL T DPSNIHHI+S NFERYPKGPDFKYIF+VLGDGIFN
Sbjct: 61  VLQAAGSTFFFKGVWFSGMDFLFTVDPSNIHHILSSNFERYPKGPDFKYIFEVLGDGIFN 120

Query: 121 SDSDSWKNQRKTAHSL--------------------GLIPVLESVCENGSVLDLQDLFQR 180
           SDSD WKNQRKTAHSL                    GL+P+LESVCENGSVLDLQDLFQR
Sbjct: 121 SDSDVWKNQRKTAHSLVHDENFLQFLEKITLKKVKEGLVPILESVCENGSVLDLQDLFQR 180

Query: 181 FSFDSTCMLVTGFDLHSLSLEFPQVPFSKAMDDVEEVIFLRHFFPKKIWELQKKLQFGEA 240
           FSFDSTCMLVTGFDL+SLSL+ P+VPFSKAMDDVEEVIFLRH FPKKIWE  KKLQ G+ 
Sbjct: 181 FSFDSTCMLVTGFDLNSLSLQLPEVPFSKAMDDVEEVIFLRHVFPKKIWEFHKKLQIGQP 240

Query: 241 KRLKQAWEIIDQTIEKLLASKRESLK-KLKEEPAEVGVGGVDLITSYITNKLEDEISNNN 300
           KRLKQAWEIID+TI KL+ASKR+S+K ++KEE  E G+ GVDLITSYITN      + N+
Sbjct: 241 KRLKQAWEIIDETIAKLIASKRKSMKNQMKEEADEQGI-GVDLITSYITN------TKND 300

Query: 301 DKFLRDTILNFMIAGRDTLSSALSWFFFCLSNNPMVEAKIREELKGIINIPTNEAHDEWR 360
           D+FLRDT+LNFMIAGRDTLSSALSWFFFCLSNNP+V A IREELK    IPTNE  D+ R
Sbjct: 301 DEFLRDTVLNFMIAGRDTLSSALSWFFFCLSNNPIVVANIREELK--TTIPTNEVRDQLR 360

Query: 361 IFSIEELKNLVYFHGALCETLRLYPPVPFQHKVPSQPDILPSGHHVNPKTKILFSLYALG 420
           IFSIEE+  LVYFHG+LCE LRLYPPVP QHKV  Q DILP+GHH+NPKTKIL SLYALG
Sbjct: 361 IFSIEEVDKLVYFHGSLCEALRLYPPVPLQHKVSVQHDILPTGHHINPKTKILISLYALG 420

Query: 421 RTSDVWGKDCMEFKPERWISENGRIKHVPSYKFLAFNAGPRTCLGKEVAFLQMKIVAAAI 480
           R SDVWGKD MEFKPERWISENGRIKHVPSYKFLAFNAGPRTCLGK+VAF ++KIVAAAI
Sbjct: 421 RMSDVWGKDSMEFKPERWISENGRIKHVPSYKFLAFNAGPRTCLGKQVAFTELKIVAAAI 480

Query: 481 IHNYNLQHIGRHKVVPSASIILHMKHGFEVMVTK 490
           IHNYN+     HKVVP+ASIILHMKHGF+V VTK
Sbjct: 481 IHNYNIIQQAGHKVVPNASIILHMKHGFKVKVTK 505

BLAST of Tan0001134 vs. NCBI nr
Match: XP_038881122.1 (alkane hydroxylase MAH1-like [Benincasa hispida])

HSP 1 Score: 754.6 bits (1947), Expect = 5.4e-214
Identity = 370/511 (72.41%), Postives = 411/511 (80.43%), Query Frame = 0

Query: 1   MASIAILSI--LLFSLLFIIFSVFVKWNPYGLPWSWPLFGMIPTIVFHIHRIHDRVTEIL 60
           MASI+ L I  +    L + F + +KWN  GLPW+WP FGM   I FH HRIHD +T+IL
Sbjct: 23  MASISFLEIPLVFIPFLLVFFLLSLKWNSNGLPWNWPFFGMTLNIFFHFHRIHDHLTQIL 82

Query: 61  KQAGSTFFFNGVWFADIDFLLTADPSNIHHIMSVNFERYPKGPDFKYIFDVLGDGIFNSD 120
            Q+ STF F GVWFAD+DF  TA+PSNIHHI+S  FERYPKGPDFKYIFD+LGDGIFNSD
Sbjct: 83  YQSSSTFLFKGVWFADLDFFFTANPSNIHHILSTKFERYPKGPDFKYIFDILGDGIFNSD 142

Query: 121 SDSWKNQRKTAHSL--------------------GLIPVLESVCENGSVLDLQDLFQRFS 180
           SD WKNQRKTAHSL                    GL+P+LESVCENGSVLDLQDLFQRFS
Sbjct: 143 SDVWKNQRKTAHSLVRDENFLQFLEKITLKKVKEGLVPILESVCENGSVLDLQDLFQRFS 202

Query: 181 FDSTCMLVTGFDLHSLSLEFPQVPFSKAMDDVEEVIFLRHFFPKKIWELQKKLQFGEAKR 240
           FDSTCMLVTGFDLHSLSLE PQVPFSKAMDD EEVIFLRHFFPK IWE QK +  G+ KR
Sbjct: 203 FDSTCMLVTGFDLHSLSLELPQVPFSKAMDDAEEVIFLRHFFPKPIWEFQKTVHIGQPKR 262

Query: 241 LKQAWEIIDQTIEKLLASKRESLKKLKEEPAEVGVGGVDLITSYITNKLEDEISNNNDKF 300
           LKQAW+IID+TI KL+ASKRESL    +E  +   GGVDLITSYITN      +N +DKF
Sbjct: 263 LKQAWKIIDETIAKLIASKRESLGNQVKEEGDHEQGGVDLITSYITN------TNKDDKF 322

Query: 301 LRDTILNFMIAGRDTLSSALSWFFFCLSNNPMVEAKIREELKGIINIPTNEAHDEWRIFS 360
           LRDT+LNFMIAGRDTLSSALSWFFFCLSNNP+VE KIR+ELK    IP NE+ DEWRIFS
Sbjct: 323 LRDTVLNFMIAGRDTLSSALSWFFFCLSNNPIVEEKIRDELK--TTIPLNESRDEWRIFS 382

Query: 361 IEELKNLVYFHGALCETLRLYPPVPFQHKVPSQPDILPSGHHVNPKTKILFSLYALGRTS 420
           IEELK LVYFHGALCE LRLYPPVP+QHKV  Q D LPSGHH+NPKTKI+FSLYALGR S
Sbjct: 383 IEELKRLVYFHGALCEALRLYPPVPYQHKVAVQHDTLPSGHHINPKTKIVFSLYALGRMS 442

Query: 421 DVWGKDCMEFKPERWISENGRIKHVPSYKFLAFNAGPRTCLGKEVAFLQMKIVAAAIIHN 480
           DVWGKDCMEFKPERWI+E G IKH PSYKFLAFNAGPRTCLGKEVAF+++K+VAA IIHN
Sbjct: 443 DVWGKDCMEFKPERWITEKGSIKHEPSYKFLAFNAGPRTCLGKEVAFIEIKLVAATIIHN 502

Query: 481 YNLQHIGRHKVVPSASIILHMKHGFEVMVTK 490
           YN+     +KVVP+ SIILHMKHGF+V +TK
Sbjct: 503 YNIIRQMGYKVVPNPSIILHMKHGFKVRITK 525

BLAST of Tan0001134 vs. NCBI nr
Match: XP_022977942.1 (alkane hydroxylase MAH1-like [Cucurbita maxima])

HSP 1 Score: 752.7 bits (1942), Expect = 2.0e-213
Identity = 372/511 (72.80%), Postives = 417/511 (81.60%), Query Frame = 0

Query: 1   MASIAILSILL--FSLLFIIFSVFVKWNPYGLPWSWPLFGMIPTIVFHIHRIHDRVTEIL 60
           MAS++ LSILL     +F +F + +KWN  GLPW+WP FGM PTI+FHIHR+H RVT IL
Sbjct: 1   MASLSFLSILLAFIPFVFALFVLSLKWNFNGLPWNWPFFGMTPTILFHIHRVHHRVTRIL 60

Query: 61  KQAGSTFFFNGVWFADIDFLLTADPSNIHHIMSVNFERYPKGPDFKYIFDVLGDGIFNSD 120
           +Q+ STF F G WFA +DF LTADPSNIHHI+S NF+RYPKGPDFKYIFDVLGDGIFNSD
Sbjct: 61  QQSPSTFLFKGAWFAGLDFFLTADPSNIHHILSTNFDRYPKGPDFKYIFDVLGDGIFNSD 120

Query: 121 SDSWKNQRKTAHSL--------------------GLIPVLESVCENGSVLDLQDLFQRFS 180
           S SWK QR TAH+L                    GL+ VLESVCENGSVLDLQDLFQRFS
Sbjct: 121 SVSWKTQRTTAHALVRQEKFLHFLEKIAMKKVREGLVSVLESVCENGSVLDLQDLFQRFS 180

Query: 181 FDSTCMLVTGFDLHSLSLEFPQVPFSKAMDDVEEVIFLRHFFPKKIWELQKKLQFGEAKR 240
           FDSTCMLVTGFDLHSLSLEFPQ+PFSKAMDD+EEVIFLRHF PKKIWE QKKLQ G   R
Sbjct: 181 FDSTCMLVTGFDLHSLSLEFPQLPFSKAMDDIEEVIFLRHFIPKKIWEFQKKLQIGHPMR 240

Query: 241 LKQAWEIIDQTIEKLLASKRESLKKLKEEPAEVGVGGVDLITSYITNKLEDEISNNNDKF 300
           L++AWEIIDQTI  L+A+KRESL +LKEE  +    GVDLITSY+ NK       N+DK 
Sbjct: 241 LQEAWEIIDQTIANLIATKRESLNQLKEEKDQ---EGVDLITSYMENK------ENDDKM 300

Query: 301 LRDTILNFMIAGRDTLSSALSWFFFCLSNNPMVEAKIREELKGIINIPTNEAHDEWRIFS 360
           LRDT+LNFMIAGRDTLSSALSWFFFCLSNNP  EAKIREEL+    IP +E   +WR+FS
Sbjct: 301 LRDTVLNFMIAGRDTLSSALSWFFFCLSNNPTAEAKIREELE--TTIPRSEPRGQWRMFS 360

Query: 361 IEELKNLVYFHGALCETLRLYPPVPFQHKVPSQPDILPSGHHVNPKTKILFSLYALGRTS 420
           I+E+  LVYFHGALCETLRLYPPVPFQHK+PSQPD+LPSG HVNPKTKI+FSLYALGR S
Sbjct: 361 IKEVDKLVYFHGALCETLRLYPPVPFQHKIPSQPDVLPSGLHVNPKTKIIFSLYALGRMS 420

Query: 421 DVWGKDCMEFKPERWISENGRIKHVPSYKFLAFNAGPRTCLGKEVAFLQMKIVAAAIIHN 480
           DVWGKDCMEFKPERWI+E GRIKHVPSYKF+AFNAGPRTC+GK+VAF +MKIVAAA+IHN
Sbjct: 421 DVWGKDCMEFKPERWIAETGRIKHVPSYKFVAFNAGPRTCIGKQVAFTEMKIVAAAMIHN 480

Query: 481 YNLQHIGRHKVVPSASIILHMKHGFEVMVTK 490
           Y +      +VVPSASIILHMKHGF+V VTK
Sbjct: 481 YAIVQETGQEVVPSASIILHMKHGFKVRVTK 500

BLAST of Tan0001134 vs. NCBI nr
Match: XP_023544417.1 (alkane hydroxylase MAH1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 749.6 bits (1934), Expect = 1.7e-212
Identity = 371/511 (72.60%), Postives = 415/511 (81.21%), Query Frame = 0

Query: 1   MASIAILSILL--FSLLFIIFSVFVKWNPYGLPWSWPLFGMIPTIVFHIHRIHDRVTEIL 60
           MAS++ LSILL     +F +  + +KWN  GLPW+WP FGM PTI+FHIHR+H R+T IL
Sbjct: 1   MASLSFLSILLAFIPFVFALVVLSLKWNFNGLPWNWPFFGMTPTILFHIHRVHHRLTHIL 60

Query: 61  KQAGSTFFFNGVWFADIDFLLTADPSNIHHIMSVNFERYPKGPDFKYIFDVLGDGIFNSD 120
           +Q+ STF F G WFA +DF LT DPSNIHHI+S NF+RYPKGPDFKYIFDVLGDGIFNSD
Sbjct: 61  QQSPSTFLFKGAWFAGLDFFLTVDPSNIHHILSTNFDRYPKGPDFKYIFDVLGDGIFNSD 120

Query: 121 SDSWKNQRKTAHSL--------------------GLIPVLESVCENGSVLDLQDLFQRFS 180
           S SWK QR TAH+L                     L+ VLESVCENGSVLDLQDLFQRFS
Sbjct: 121 SVSWKTQRTTAHALVRQEKFLHFLEKIAMKKVREELVSVLESVCENGSVLDLQDLFQRFS 180

Query: 181 FDSTCMLVTGFDLHSLSLEFPQVPFSKAMDDVEEVIFLRHFFPKKIWELQKKLQFGEAKR 240
           FDSTCMLVTGFDLHSLSLEFPQVPFSKAMDD+EEVIFLRHF PKKIWE QKKLQ G   R
Sbjct: 181 FDSTCMLVTGFDLHSLSLEFPQVPFSKAMDDIEEVIFLRHFIPKKIWEFQKKLQIGHPMR 240

Query: 241 LKQAWEIIDQTIEKLLASKRESLKKLKEEPAEVGVGGVDLITSYITNKLEDEISNNNDKF 300
           L++AWEIIDQTI  L+A+KRESL +L+EE  E    GVDLITSY+ NK       N+DK 
Sbjct: 241 LQEAWEIIDQTIANLIATKRESLNQLREEKDE---EGVDLITSYMENK------ENDDKM 300

Query: 301 LRDTILNFMIAGRDTLSSALSWFFFCLSNNPMVEAKIREELKGIINIPTNEAHDEWRIFS 360
           LRDT+LNFMIAGRDTLSSALSWFFFCLSNNP VEAKIREEL+    IPT+E   +WR+FS
Sbjct: 301 LRDTVLNFMIAGRDTLSSALSWFFFCLSNNPTVEAKIREELE--TTIPTSEPRGQWRMFS 360

Query: 361 IEELKNLVYFHGALCETLRLYPPVPFQHKVPSQPDILPSGHHVNPKTKILFSLYALGRTS 420
           I+E+  LVYFHGALCETLRLYPPVPFQHK+PSQPD+LPSGHHVNPKTKI+FSLYALGR S
Sbjct: 361 IKEVDKLVYFHGALCETLRLYPPVPFQHKIPSQPDVLPSGHHVNPKTKIIFSLYALGRMS 420

Query: 421 DVWGKDCMEFKPERWISENGRIKHVPSYKFLAFNAGPRTCLGKEVAFLQMKIVAAAIIHN 480
           DVWGKDCMEFKPERW+SE GRI HVPS+KFLAFNAGPRTCLGK+VAF +MKIVAAA+IHN
Sbjct: 421 DVWGKDCMEFKPERWVSEKGRIMHVPSHKFLAFNAGPRTCLGKQVAFTEMKIVAAAMIHN 480

Query: 481 YNLQHIGRHKVVPSASIILHMKHGFEVMVTK 490
           Y +      +VVPSASIILHMKHG +V VTK
Sbjct: 481 YAIVQETGQEVVPSASIILHMKHGLKVRVTK 500

BLAST of Tan0001134 vs. NCBI nr
Match: XP_022950281.1 (alkane hydroxylase MAH1-like [Cucurbita moschata])

HSP 1 Score: 747.3 bits (1928), Expect = 8.6e-212
Identity = 370/511 (72.41%), Postives = 415/511 (81.21%), Query Frame = 0

Query: 1   MASIAILSILL--FSLLFIIFSVFVKWNPYGLPWSWPLFGMIPTIVFHIHRIHDRVTEIL 60
           MAS++ LSILL     +F +F + +KWN  GLPW+WP FGM PTI+FHIHR+H R+T IL
Sbjct: 34  MASLSFLSILLAFIPFVFALFVLSLKWNFNGLPWNWPFFGMTPTILFHIHRVHHRLTRIL 93

Query: 61  KQAGSTFFFNGVWFADIDFLLTADPSNIHHIMSVNFERYPKGPDFKYIFDVLGDGIFNSD 120
           +Q+ STF F G WFA +DF LTADPSNIHHI+S NF+RYPKG DFKY+FDVLGDGI NSD
Sbjct: 94  QQSPSTFLFKGAWFAGLDFFLTADPSNIHHILSTNFDRYPKGTDFKYMFDVLGDGISNSD 153

Query: 121 SDSWKNQRKTAHSL--------------------GLIPVLESVCENGSVLDLQDLFQRFS 180
           S SWK QR TAH+L                    GL+ VLESVCENGSVLDLQDLFQRFS
Sbjct: 154 SVSWKTQRTTAHALVRQEKFLHFLERIAMKKVREGLVSVLESVCENGSVLDLQDLFQRFS 213

Query: 181 FDSTCMLVTGFDLHSLSLEFPQVPFSKAMDDVEEVIFLRHFFPKKIWELQKKLQFGEAKR 240
           FDS+CMLVTG DLHSLSLEFPQVPFSKAMDD+EEVIFLRH  PKKIWE QKKLQ G  KR
Sbjct: 214 FDSSCMLVTGLDLHSLSLEFPQVPFSKAMDDIEEVIFLRHLIPKKIWEFQKKLQIGHPKR 273

Query: 241 LKQAWEIIDQTIEKLLASKRESLKKLKEEPAEVGVGGVDLITSYITNKLEDEISNNNDKF 300
           L++AWEIIDQTI  L+A+KRESL +L+EE  +    GVDLITSY+ NK       N+DK 
Sbjct: 274 LQEAWEIIDQTIANLIATKRESLNQLREEKDQ---EGVDLITSYMENK------ENDDKM 333

Query: 301 LRDTILNFMIAGRDTLSSALSWFFFCLSNNPMVEAKIREELKGIINIPTNEAHDEWRIFS 360
           LRDT+LNF+IAGRDTLSSALSWFFFCLSNNP VEAKIREEL+    IPT+E    WR+FS
Sbjct: 334 LRDTVLNFVIAGRDTLSSALSWFFFCLSNNPTVEAKIREELE--TTIPTSEPRGRWRMFS 393

Query: 361 IEELKNLVYFHGALCETLRLYPPVPFQHKVPSQPDILPSGHHVNPKTKILFSLYALGRTS 420
           I+E+  LVYFHGALCETLRLYPPVPFQHK+PSQPD+LPSGHHVNPKTKI+FSLYALGR S
Sbjct: 394 IKEVDKLVYFHGALCETLRLYPPVPFQHKIPSQPDVLPSGHHVNPKTKIIFSLYALGRMS 453

Query: 421 DVWGKDCMEFKPERWISENGRIKHVPSYKFLAFNAGPRTCLGKEVAFLQMKIVAAAIIHN 480
           DVWGKDCMEFKPERWISE G IKHVPSYKFLAFNAGPRTCLGK+VAF++MKIVAAA+IHN
Sbjct: 454 DVWGKDCMEFKPERWISETGMIKHVPSYKFLAFNAGPRTCLGKQVAFIEMKIVAAAMIHN 513

Query: 481 YNLQHIGRHKVVPSASIILHMKHGFEVMVTK 490
           Y +       VVPSASIILHMKHGF+V VTK
Sbjct: 514 YAIVQQTGQAVVPSASIILHMKHGFKVRVTK 533

BLAST of Tan0001134 vs. ExPASy TrEMBL
Match: A0A6J1IJU0 (alkane hydroxylase MAH1-like OS=Cucurbita maxima OX=3661 GN=LOC111478084 PE=3 SV=1)

HSP 1 Score: 752.7 bits (1942), Expect = 9.9e-214
Identity = 372/511 (72.80%), Postives = 417/511 (81.60%), Query Frame = 0

Query: 1   MASIAILSILL--FSLLFIIFSVFVKWNPYGLPWSWPLFGMIPTIVFHIHRIHDRVTEIL 60
           MAS++ LSILL     +F +F + +KWN  GLPW+WP FGM PTI+FHIHR+H RVT IL
Sbjct: 1   MASLSFLSILLAFIPFVFALFVLSLKWNFNGLPWNWPFFGMTPTILFHIHRVHHRVTRIL 60

Query: 61  KQAGSTFFFNGVWFADIDFLLTADPSNIHHIMSVNFERYPKGPDFKYIFDVLGDGIFNSD 120
           +Q+ STF F G WFA +DF LTADPSNIHHI+S NF+RYPKGPDFKYIFDVLGDGIFNSD
Sbjct: 61  QQSPSTFLFKGAWFAGLDFFLTADPSNIHHILSTNFDRYPKGPDFKYIFDVLGDGIFNSD 120

Query: 121 SDSWKNQRKTAHSL--------------------GLIPVLESVCENGSVLDLQDLFQRFS 180
           S SWK QR TAH+L                    GL+ VLESVCENGSVLDLQDLFQRFS
Sbjct: 121 SVSWKTQRTTAHALVRQEKFLHFLEKIAMKKVREGLVSVLESVCENGSVLDLQDLFQRFS 180

Query: 181 FDSTCMLVTGFDLHSLSLEFPQVPFSKAMDDVEEVIFLRHFFPKKIWELQKKLQFGEAKR 240
           FDSTCMLVTGFDLHSLSLEFPQ+PFSKAMDD+EEVIFLRHF PKKIWE QKKLQ G   R
Sbjct: 181 FDSTCMLVTGFDLHSLSLEFPQLPFSKAMDDIEEVIFLRHFIPKKIWEFQKKLQIGHPMR 240

Query: 241 LKQAWEIIDQTIEKLLASKRESLKKLKEEPAEVGVGGVDLITSYITNKLEDEISNNNDKF 300
           L++AWEIIDQTI  L+A+KRESL +LKEE  +    GVDLITSY+ NK       N+DK 
Sbjct: 241 LQEAWEIIDQTIANLIATKRESLNQLKEEKDQ---EGVDLITSYMENK------ENDDKM 300

Query: 301 LRDTILNFMIAGRDTLSSALSWFFFCLSNNPMVEAKIREELKGIINIPTNEAHDEWRIFS 360
           LRDT+LNFMIAGRDTLSSALSWFFFCLSNNP  EAKIREEL+    IP +E   +WR+FS
Sbjct: 301 LRDTVLNFMIAGRDTLSSALSWFFFCLSNNPTAEAKIREELE--TTIPRSEPRGQWRMFS 360

Query: 361 IEELKNLVYFHGALCETLRLYPPVPFQHKVPSQPDILPSGHHVNPKTKILFSLYALGRTS 420
           I+E+  LVYFHGALCETLRLYPPVPFQHK+PSQPD+LPSG HVNPKTKI+FSLYALGR S
Sbjct: 361 IKEVDKLVYFHGALCETLRLYPPVPFQHKIPSQPDVLPSGLHVNPKTKIIFSLYALGRMS 420

Query: 421 DVWGKDCMEFKPERWISENGRIKHVPSYKFLAFNAGPRTCLGKEVAFLQMKIVAAAIIHN 480
           DVWGKDCMEFKPERWI+E GRIKHVPSYKF+AFNAGPRTC+GK+VAF +MKIVAAA+IHN
Sbjct: 421 DVWGKDCMEFKPERWIAETGRIKHVPSYKFVAFNAGPRTCIGKQVAFTEMKIVAAAMIHN 480

Query: 481 YNLQHIGRHKVVPSASIILHMKHGFEVMVTK 490
           Y +      +VVPSASIILHMKHGF+V VTK
Sbjct: 481 YAIVQETGQEVVPSASIILHMKHGFKVRVTK 500

BLAST of Tan0001134 vs. ExPASy TrEMBL
Match: A0A6J1GEE6 (alkane hydroxylase MAH1-like OS=Cucurbita moschata OX=3662 GN=LOC111453415 PE=3 SV=1)

HSP 1 Score: 747.3 bits (1928), Expect = 4.1e-212
Identity = 370/511 (72.41%), Postives = 415/511 (81.21%), Query Frame = 0

Query: 1   MASIAILSILL--FSLLFIIFSVFVKWNPYGLPWSWPLFGMIPTIVFHIHRIHDRVTEIL 60
           MAS++ LSILL     +F +F + +KWN  GLPW+WP FGM PTI+FHIHR+H R+T IL
Sbjct: 34  MASLSFLSILLAFIPFVFALFVLSLKWNFNGLPWNWPFFGMTPTILFHIHRVHHRLTRIL 93

Query: 61  KQAGSTFFFNGVWFADIDFLLTADPSNIHHIMSVNFERYPKGPDFKYIFDVLGDGIFNSD 120
           +Q+ STF F G WFA +DF LTADPSNIHHI+S NF+RYPKG DFKY+FDVLGDGI NSD
Sbjct: 94  QQSPSTFLFKGAWFAGLDFFLTADPSNIHHILSTNFDRYPKGTDFKYMFDVLGDGISNSD 153

Query: 121 SDSWKNQRKTAHSL--------------------GLIPVLESVCENGSVLDLQDLFQRFS 180
           S SWK QR TAH+L                    GL+ VLESVCENGSVLDLQDLFQRFS
Sbjct: 154 SVSWKTQRTTAHALVRQEKFLHFLERIAMKKVREGLVSVLESVCENGSVLDLQDLFQRFS 213

Query: 181 FDSTCMLVTGFDLHSLSLEFPQVPFSKAMDDVEEVIFLRHFFPKKIWELQKKLQFGEAKR 240
           FDS+CMLVTG DLHSLSLEFPQVPFSKAMDD+EEVIFLRH  PKKIWE QKKLQ G  KR
Sbjct: 214 FDSSCMLVTGLDLHSLSLEFPQVPFSKAMDDIEEVIFLRHLIPKKIWEFQKKLQIGHPKR 273

Query: 241 LKQAWEIIDQTIEKLLASKRESLKKLKEEPAEVGVGGVDLITSYITNKLEDEISNNNDKF 300
           L++AWEIIDQTI  L+A+KRESL +L+EE  +    GVDLITSY+ NK       N+DK 
Sbjct: 274 LQEAWEIIDQTIANLIATKRESLNQLREEKDQ---EGVDLITSYMENK------ENDDKM 333

Query: 301 LRDTILNFMIAGRDTLSSALSWFFFCLSNNPMVEAKIREELKGIINIPTNEAHDEWRIFS 360
           LRDT+LNF+IAGRDTLSSALSWFFFCLSNNP VEAKIREEL+    IPT+E    WR+FS
Sbjct: 334 LRDTVLNFVIAGRDTLSSALSWFFFCLSNNPTVEAKIREELE--TTIPTSEPRGRWRMFS 393

Query: 361 IEELKNLVYFHGALCETLRLYPPVPFQHKVPSQPDILPSGHHVNPKTKILFSLYALGRTS 420
           I+E+  LVYFHGALCETLRLYPPVPFQHK+PSQPD+LPSGHHVNPKTKI+FSLYALGR S
Sbjct: 394 IKEVDKLVYFHGALCETLRLYPPVPFQHKIPSQPDVLPSGHHVNPKTKIIFSLYALGRMS 453

Query: 421 DVWGKDCMEFKPERWISENGRIKHVPSYKFLAFNAGPRTCLGKEVAFLQMKIVAAAIIHN 480
           DVWGKDCMEFKPERWISE G IKHVPSYKFLAFNAGPRTCLGK+VAF++MKIVAAA+IHN
Sbjct: 454 DVWGKDCMEFKPERWISETGMIKHVPSYKFLAFNAGPRTCLGKQVAFIEMKIVAAAMIHN 513

Query: 481 YNLQHIGRHKVVPSASIILHMKHGFEVMVTK 490
           Y +       VVPSASIILHMKHGF+V VTK
Sbjct: 514 YAIVQQTGQAVVPSASIILHMKHGFKVRVTK 533

BLAST of Tan0001134 vs. ExPASy TrEMBL
Match: A0A0A0KJ78 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G487530 PE=3 SV=1)

HSP 1 Score: 741.5 bits (1913), Expect = 2.3e-210
Identity = 371/515 (72.04%), Postives = 418/515 (81.17%), Query Frame = 0

Query: 1   MASIAILSIL----LFSLLFIIFSVFVKWNPYG-LPWSWPLFGMIPTIVFHIHRIHDRVT 60
           MASI   SIL    +  L+FII S   +W+ +G +PW+WP+ GM PT++ HIHR HDRVT
Sbjct: 1   MASIDFSSILFAFIIPFLVFIILSRIWRWDFHGIIPWNWPIVGMTPTVIAHIHRPHDRVT 60

Query: 61  EILKQAGSTFFFNGVWFADIDFLLTADPSNIHHIMSVNFERYPKGPDFKYIFDVLGDGIF 120
           E+L+Q   TFFF G+WF+ +DFL T DPSNIHHI+S NFERYPKGPDFKYIF+VLGDGIF
Sbjct: 61  EVLQQTAFTFFFKGIWFSGMDFLFTVDPSNIHHILSANFERYPKGPDFKYIFEVLGDGIF 120

Query: 121 NSDSDSWKNQRKTAHSL--------------------GLIPVLESVCENGSVLDLQDLFQ 180
           NSDSD WKNQRKTAH L                    GL+P+L+SVCENGSVLDLQDLFQ
Sbjct: 121 NSDSDVWKNQRKTAHYLVRHHNFLQFLEKITLNKVKTGLVPILDSVCENGSVLDLQDLFQ 180

Query: 181 RFSFDSTCMLVTGFDLHSLSLEFPQVPFSKAMDDVEEVIFLRHFFPKKIWELQKKLQFGE 240
           RFSFDSTCM+VTGFDL+SLSLEFP+VPFSKAMDD EEVIF+RHFFPK IWE QKKLQ G+
Sbjct: 181 RFSFDSTCMMVTGFDLNSLSLEFPEVPFSKAMDDAEEVIFVRHFFPKTIWEFQKKLQIGQ 240

Query: 241 AKRLKQAWEIIDQTIEKLLASKRESLKKLKEEPAEVGVGGVDLITSYITNKLEDEISNNN 300
            KRLKQAWEIID+TI KL+ASKR+SLK   +E  + G  GVDLITSYITN        N+
Sbjct: 241 PKRLKQAWEIIDETIAKLIASKRKSLKNEMKEEDDNGKEGVDLITSYITN------IEND 300

Query: 301 DKFLRDTILNFMIAGRDTLSSALSWFFFCLSNNPMVEAKIREELKGIINIPTNEAHDEWR 360
           D+FLRDT+LNFMIAGRDTLSSALSWFFFCLSN+P V  KIREEL+    IPTNEA+D+ R
Sbjct: 301 DEFLRDTVLNFMIAGRDTLSSALSWFFFCLSNHPTVVEKIREELR--TTIPTNEAYDQRR 360

Query: 361 IFSIEELKNLVYFHGALCETLRLYPPVPFQHKVPSQPDILPSGHHVNPKTKILFSLYALG 420
           IFSIEE+ NLVYFHG LCE LRLYPPVP QHKV  Q DILPSGHH+ PKTKILFSLYALG
Sbjct: 361 IFSIEEVDNLVYFHGTLCEALRLYPPVPLQHKVSIQHDILPSGHHIKPKTKILFSLYALG 420

Query: 421 RTSDVWGKDCMEFKPERWI-SENGRIKHVPSYKFLAFNAGPRTCLGKEVAFLQMKIVAAA 480
           R S+VWGKDC+EFKPERWI SENG+IKHVPSYKFLAFNAGPRTCLGK VAF ++KIVAAA
Sbjct: 421 RMSEVWGKDCLEFKPERWINSENGKIKHVPSYKFLAFNAGPRTCLGKHVAFTELKIVAAA 480

Query: 481 IIHNYNLQHIGRHKVVPSASIILHMKHGFEVMVTK 490
           IIHNYN+     H+VVPSASIILHMKHGF+V VTK
Sbjct: 481 IIHNYNIIQQAGHEVVPSASIILHMKHGFKVKVTK 507

BLAST of Tan0001134 vs. ExPASy TrEMBL
Match: A0A6J1BWK7 (alkane hydroxylase MAH1-like OS=Momordica charantia OX=3673 GN=LOC111005984 PE=3 SV=1)

HSP 1 Score: 741.1 bits (1912), Expect = 3.0e-210
Identity = 375/514 (72.96%), Postives = 421/514 (81.91%), Query Frame = 0

Query: 1   MASIAILSILLFSL---LFIIFSVFVKWNPYGLPWSWPLFGMIPTIVFHIHRIHDRVTEI 60
           MA I+ILSILL SL     +IF + + WNP+G+PW+WP+ GMIPTI  ++HRIHDRVTEI
Sbjct: 1   MAPISILSILLPSLPLIFILIFFISLNWNPHGIPWNWPILGMIPTIFSNLHRIHDRVTEI 60

Query: 61  LKQAGSTFFFNGVWFADIDFLLTADPSNIHHIMSVNFERYPKGPDFKYIFDVLGDGIFNS 120
           L Q+ STFFF  +WFA+IDFLLTADPSNIHHI+S NF+RYPKGPDF YIFDVLGDGIFN+
Sbjct: 61  LHQSSSTFFFKAIWFANIDFLLTADPSNIHHILSANFQRYPKGPDFNYIFDVLGDGIFNA 120

Query: 121 DSDSWKNQRKTAHSL--------------------GLIPVLESVCENGSVLDLQDLFQRF 180
           DSDSWK QRK AHSL                    G++ VLESVCENGSVLDLQDLFQRF
Sbjct: 121 DSDSWKYQRKIAHSLVHRDEFLRFLEKNTMKKVKEGVVSVLESVCENGSVLDLQDLFQRF 180

Query: 181 SFDSTCMLVTGFDLHSLSLEFPQVPFSKAMDDVEEVIFLRHFFPKKIWELQKKLQFGEAK 240
           SFDSTCMLVTGFDL+SLSL+FPQVPFSKAMDDVEE+IFLRHFFPK +WEL  KLQ G+ +
Sbjct: 181 SFDSTCMLVTGFDLNSLSLDFPQVPFSKAMDDVEEIIFLRHFFPKALWELLNKLQIGQPR 240

Query: 241 RLKQAWEIIDQTIEKLLASKRESLKKLKEEPAEVGVGGVDLITSY-ITNKLEDEISNNND 300
           R KQA +IIDQTI +L+A KRESL+  KEE A+    G DLITSY I  + +D     N+
Sbjct: 241 RQKQARQIIDQTIAELIALKRESLQ--KEEAAQ----GADLITSYMIDEEYQDGNFEKNE 300

Query: 301 KFLRDTILNFMIAGRDTLSSALSWFFFCLSNNPMVEAKIREELKGIINIPTNEAHDEWRI 360
            FLRDT+LNFMIAGRDTLSSALSWFFFCLSNNP VEAKIREELK I  IP NE HD+WRI
Sbjct: 301 NFLRDTVLNFMIAGRDTLSSALSWFFFCLSNNPTVEAKIREELKSI--IPENEDHDQWRI 360

Query: 361 FSIEELKNLVYFHGALCETLRLYPPVPFQHKVPSQPDILPSGHHVNPKTKILFSLYALGR 420
           FSIEEL  LVYFHGALCETLRLYPPVPFQHKV  + D+LPSG  V+P TKILFSLYALGR
Sbjct: 361 FSIEELNKLVYFHGALCETLRLYPPVPFQHKVXVEADVLPSGXKVDPTTKILFSLYALGR 420

Query: 421 TSDVWGKDCMEFKPERWISENGRIKHVPSYKFLAFNAGPRTCLGKEVAFLQMKIVAAAII 480
            S+VWGKDC EFKPERWISE G IKHVPSYKFLAFNAGPRTCLGK+VAF ++K+VAAAII
Sbjct: 421 MSEVWGKDCEEFKPERWISEKGSIKHVPSYKFLAFNAGPRTCLGKQVAFTEIKMVAAAII 480

Query: 481 HNYN-LQHIGRHKVVPSASIILHMKHGFEVMVTK 490
           HNYN +Q IG HKVVP+ SIILHMKHGF+V +TK
Sbjct: 481 HNYNIIQEIG-HKVVPNPSIILHMKHGFKVKITK 505

BLAST of Tan0001134 vs. ExPASy TrEMBL
Match: A0A6J1BTU4 (alkane hydroxylase MAH1-like OS=Momordica charantia OX=3673 GN=LOC111005299 PE=3 SV=1)

HSP 1 Score: 740.3 bits (1910), Expect = 5.1e-210
Identity = 365/497 (73.44%), Postives = 410/497 (82.49%), Query Frame = 0

Query: 15  LFIIFSVFVKWNPYG-LPWSWPLFGMIPTIVFHIHRIHDRVTEILKQAGSTFFFNGVWFA 74
           L +IF + +KWN  G +PW+WPL GM PT+  HIHR HDR+TE+L Q G +F F G+WF 
Sbjct: 21  LLMIFFLILKWNFDGFVPWNWPLVGMTPTVACHIHRAHDRITEVLNQTGCSFLFKGIWFT 80

Query: 75  DIDFLLTADPSNIHHIMSVNFERYPKGPDFKYIFDVLGDGIFNSDSDSWKNQRKTAHSL- 134
           D+DFLLTADPSNIHHI+S NFERYPKGPDFKYIFDVLGDGIFNSDSDSW+ QR+ AHSL 
Sbjct: 81  DLDFLLTADPSNIHHILSANFERYPKGPDFKYIFDVLGDGIFNSDSDSWREQRRIAHSLV 140

Query: 135 -------------------GLIPVLESVCENGSVLDLQDLFQRFSFDSTCMLVTGFDLHS 194
                              G++ VLESVCE+GSVLDLQDLFQRFSFDSTCMLVTGFD +S
Sbjct: 141 RHDKFLQFLEETTMKKVKEGVVLVLESVCEDGSVLDLQDLFQRFSFDSTCMLVTGFDFNS 200

Query: 195 LSLEFPQVPFSKAMDDVEEVIFLRHFFPKKIWELQKKLQFGEAKRLKQAWEIIDQTIEKL 254
           LS++FPQVPFSKAMDDVEEVI LRHF PK +WELQKKLQ G+ K+LK+AWEIIDQTI KL
Sbjct: 201 LSIDFPQVPFSKAMDDVEEVILLRHFIPKSLWELQKKLQIGQPKKLKEAWEIIDQTIAKL 260

Query: 255 LASKRESLKKLKEEPAEVGVGGVDLITSYITNKLEDEISNNNDKFLRDTILNFMIAGRDT 314
           +A KRESL+  KEEPA+    G DLITSY+ N  E++    N+ FLRDT+LNFMIAGRDT
Sbjct: 261 IALKRESLQ--KEEPAQ----GADLITSYMVN--EEDGFEKNENFLRDTVLNFMIAGRDT 320

Query: 315 LSSALSWFFFCLSNNPMVEAKIREELKGIINIPTNEAHDEWRIFSIEELKNLVYFHGALC 374
           LSSALSWFFFCLSNNP VEAKIREELK I  IP NE HD+WRIFSIEEL  LVYFHGALC
Sbjct: 321 LSSALSWFFFCLSNNPTVEAKIREELKSI--IPENEDHDQWRIFSIEELNKLVYFHGALC 380

Query: 375 ETLRLYPPVPFQHKVPSQPDILPSGHHVNPKTKILFSLYALGRTSDVWGKDCMEFKPERW 434
           ETLRLYPPVPFQHKV  + D+LPSGH V+P TKILFSLYALGR S+VWGKDC EFKPERW
Sbjct: 381 ETLRLYPPVPFQHKVTVEADVLPSGHKVDPTTKILFSLYALGRMSEVWGKDCEEFKPERW 440

Query: 435 ISENGRIKHVPSYKFLAFNAGPRTCLGKEVAFLQMKIVAAAIIHNYN-LQHIGRHKVVPS 490
           ISE G IKHVPSYKFLAFNAGPRTCLGK+VAF ++K+VAAAIIHNYN +Q IG HKVVP+
Sbjct: 441 ISEKGSIKHVPSYKFLAFNAGPRTCLGKQVAFTEIKMVAAAIIHNYNIIQEIG-HKVVPN 500

BLAST of Tan0001134 vs. TAIR 10
Match: AT2G23180.1 (cytochrome P450, family 96, subfamily A, polypeptide 1 )

HSP 1 Score: 488.4 bits (1256), Expect = 6.7e-138
Identity = 250/510 (49.02%), Postives = 340/510 (66.67%), Query Frame = 0

Query: 7   LSILLFSLLFIIFSVFVKWNPYGLPWSWPLFGMIPTIVFHIHRIHDRVTEILKQAGSTFF 66
           +S+L FS L+  F +  K +   L  +WP  GM+P ++  I R++D VTE+L+ +  T+ 
Sbjct: 11  ISLLFFSFLYGYFLISKKPHRSFLT-NWPFLGMLPGLLVEIPRVYDFVTELLEASNLTYP 70

Query: 67  FNGVWFADIDFLLTADPSNIHHIMSVNFERYPKGPDFKYIFDVLGDGIFNSDSDSWKNQR 126
           F G  F  +D L+T DP+NIHHIMS NF  YPKG +FK IFDVLGDGIFN+DS+ WK+ R
Sbjct: 71  FKGPCFGGLDMLITVDPANIHHIMSSNFANYPKGTEFKKIFDVLGDGIFNADSELWKDLR 130

Query: 127 KTAHSL--------------------GLIPVLESVCENGSVLDLQDLFQRFSFDSTCMLV 186
           K+A S+                    GL+P+L+ V E   V+DLQD+FQRF+FD++ +L 
Sbjct: 131 KSAQSMMTHQDFQRFTLRTIMSKLEKGLVPLLDYVAEKKQVVDLQDVFQRFTFDTSFVLA 190

Query: 187 TGFDLHSLSLEFPQVPFSKAMDDVEEVIFLRHFFPKKIWELQKKLQFGEAKRLKQAWEII 246
           TG D   LS E PQ+ F++A+D+ EE IF RH  P+ +W++Q+ + FG+  ++K+A    
Sbjct: 191 TGVDPGCLSTEMPQIEFARALDEAEEAIFFRHVKPEIVWKMQRFIGFGDELKMKKAHSTF 250

Query: 247 DQTIEKLLASKRESLKKLKEEPAEVGVGGVDLITSYI-------TNKLEDEISNNNDKFL 306
           D+   K +ASKR+   ++      +     DL+  Y+       T K +  ++ ++DKFL
Sbjct: 251 DRVCSKCIASKRD---EITNGVINIDSSSKDLLMCYMNVDTICHTTKYK-LLNPSDDKFL 310

Query: 307 RDTILNFMIAGRDTLSSALSWFFFCLSNNPMVEAKIREELKGIINIPTNEAHDEWRIFSI 366
           RD IL+FM+AGRDT SSAL+WFF+ LS NP    KIR+E+   ++  TN    ++  F+ 
Sbjct: 311 RDMILSFMLAGRDTTSSALTWFFWLLSKNPKAITKIRQEINTQLSPRTN----DFDSFNA 370

Query: 367 EELKNLVYFHGALCETLRLYPPVPFQHKVPSQPDILPSGHHVNPKTKILFSLYALGRTSD 426
           +EL  LVY HGALCE LRLYPPVPFQHK P++ D+LPSGH V+  +KI+F LY+LGR   
Sbjct: 371 QELNKLVYVHGALCEALRLYPPVPFQHKSPTKSDVLPSGHRVDASSKIVFCLYSLGRMKS 430

Query: 427 VWGKDCMEFKPERWISENGRIKHVPSYKFLAFNAGPRTCLGKEVAFLQMKIVAAAIIHNY 486
           VWG+D  EFKPERWISE+GR+ HVPS+KFL+FNAGPRTCLGKEVA  QMK VA  II NY
Sbjct: 431 VWGEDASEFKPERWISESGRLIHVPSFKFLSFNAGPRTCLGKEVAMTQMKTVAVKIIQNY 490

Query: 487 NLQHIGRHKVVPSASIILHMKHGFEVMVTK 490
            ++ +  HK+ P  SIILHMKHG +V VTK
Sbjct: 491 EIKVVEGHKIEPVPSIILHMKHGLKVTVTK 511

BLAST of Tan0001134 vs. TAIR 10
Match: AT4G39490.1 (cytochrome P450, family 96, subfamily A, polypeptide 10 )

HSP 1 Score: 487.6 bits (1254), Expect = 1.1e-137
Identity = 252/522 (48.28%), Postives = 342/522 (65.52%), Query Frame = 0

Query: 1   MASIAILSILLFSLLFIIFSVFV--KWNPYGLPWSWPLFGMIPTIVFHIHRIHDRVTEIL 60
           M  I++  I +    F++F  F+  K      P +WP FGMIP ++  IHR++D +TEIL
Sbjct: 1   MVFISLFEISIAFFCFLLFRHFLINKKTHRLCPTNWPFFGMIPGLLVEIHRVYDFITEIL 60

Query: 61  KQAGSTFFFNGVWFADIDFLLTADPSNIHHIMSVNFERYPKGPDFKYIFDVLGDGIFNSD 120
           +    T+   G  FA++D L+T DP+NIHHIMS NF  YPKGP+FK +FD+LGDGIFN+D
Sbjct: 61  EVTNLTYPCTGPCFANLDMLVTVDPANIHHIMSSNFANYPKGPEFKKLFDILGDGIFNAD 120

Query: 121 SDSWKNQRKTAHSL--------------------GLIPVLESVCENGSVLDLQDLFQRFS 180
           S+ WK+ RK+A S+                    GL+P+L+ V +    +DLQD+FQRF+
Sbjct: 121 SELWKDLRKSAQSMMMNPEFQKFSLATSLKKLEKGLVPLLDHVAKEKLAVDLQDMFQRFT 180

Query: 181 FDSTCMLVTGFDLHSLSLEFPQVPFSKAMDDVEEVIFLRHFFPKKIWELQKKLQFGEAKR 240
           FD+T +L TG+D   LS+E P+V F++A+DD EE IF RH  P+  W LQ  L  G+ K+
Sbjct: 181 FDTTFVLATGYDPGCLSVEMPEVEFARALDDAEEAIFFRHVKPEIFWRLQGLLGLGDEKK 240

Query: 241 LKQAWEIIDQTIEKLLASKRESLKKLKEEPAEVGVGGVDLITSYI---TNKLEDEISNNN 300
           + +A   +D+   K +A KR+ + +       V     DL+TSY+   T K +  ++ ++
Sbjct: 241 MTKARSTLDRVCSKYIAIKRDEVSR---GTNNVDSHSKDLLTSYMNLDTTKYK-LLNPSD 300

Query: 301 DKFLRDTILNFMIAGRDTLSSALSWFFFCLSNNPMVEAKIREELKGII--------NIPT 360
           ++FLRDTIL FM+AGRDT  S L+WFF+ L  NP V AKIR+E+   +        +   
Sbjct: 301 ERFLRDTILTFMLAGRDTTGSGLTWFFWLLIKNPEVIAKIRQEINTALFQRSKVDDDASN 360

Query: 361 NEAHDEWRIFSIEELKNLVYFHGALCETLRLYPPVPFQHKVPSQPDILPSGHHVNPKTKI 420
           N   D    FS +ELK LVY HGA+CE+LRLYPPVPFQHK P++PD+LPSGH V+  +KI
Sbjct: 361 NNDSDS---FSPQELKKLVYLHGAICESLRLYPPVPFQHKSPTKPDVLPSGHKVDANSKI 420

Query: 421 LFSLYALGRTSDVWGKDCMEFKPERWISENGRIKHVPSYKFLAFNAGPRTCLGKEVAFLQ 480
           LF LY+LGR   VWG+D +EFKPERWISE+G   H PSYKFL+FNAGPRTCLGKEVA +Q
Sbjct: 421 LFCLYSLGRMKSVWGEDALEFKPERWISESGNSVHEPSYKFLSFNAGPRTCLGKEVAMMQ 480

Query: 481 MKIVAAAIIHNYNLQHIGRHKVVPSASIILHMKHGFEVMVTK 490
           MK VA  II NY ++ +   ++ P+ S+ILHMKHG +V VTK
Sbjct: 481 MKSVAVKIIQNYEMKIVEGQQIEPAPSVILHMKHGLKVTVTK 515

BLAST of Tan0001134 vs. TAIR 10
Match: AT4G39480.1 (cytochrome P450, family 96, subfamily A, polypeptide 9 )

HSP 1 Score: 485.0 bits (1247), Expect = 7.4e-137
Identity = 248/519 (47.78%), Postives = 344/519 (66.28%), Query Frame = 0

Query: 1   MASIAILSILLFSLLFIIFSVF-VKWNPY-GLPWSWPLFGMIPTIVFHIHRIHDRVTEIL 60
           MA +++L I +    F+IF +F +   P+     +WPL GM+P ++  + R++D +TE+L
Sbjct: 1   MALVSLLEISIAFFCFLIFRIFLISKKPHRSFLTNWPLLGMLPGLLTVLPRVYDFITEVL 60

Query: 61  KQAGSTFFFNGVWFADIDFLLTADPSNIHHIMSVNFERYPKGPDFKYIFDVLGDGIFNSD 120
           +     + F G +   ID L T DP+NIHHIMS NF  YPKG +FK +FDVLGDGIFN+D
Sbjct: 61  EDGNLNYLFIGPFLGGIDMLFTVDPANIHHIMSSNFANYPKGTEFKKLFDVLGDGIFNAD 120

Query: 121 SDSWKNQRKTAHSL--------------------GLIPVLESVCENGSVLDLQDLFQRFS 180
           SD WK+ RK++ S+                    GL+P+L+ V +   V+DLQD+FQRF+
Sbjct: 121 SDLWKDLRKSSQSMMNHPDFQRFSLATSLSKLEKGLVPLLDHVAKEKLVVDLQDVFQRFT 180

Query: 181 FDSTCMLVTGFDLHSLSLEFPQVPFSKAMDDVEEVIFLRHFFPKKIWELQKKLQFGEAKR 240
           FD+T +L TG+D   LS+E P++ F++A+DD EE IF RHF P+ +W++Q+ +  G   +
Sbjct: 181 FDTTFVLATGYDPGCLSVEMPEIEFARALDDAEEAIFYRHFKPEVVWKMQRLIGVGAELK 240

Query: 241 LKQAWEIIDQTIEKLLASKRESLKKLKEEPAEVGVGGVDLITSYI---TNKLEDEISNNN 300
           LK+A  I D+   K +ASKR+ + +  +  +       DL+ S I   T K +  ++ ++
Sbjct: 241 LKRAHAIFDRVCSKCIASKRDEISQGIDSSS-----SKDLLMSSINVDTTKYK-LLNPSD 300

Query: 301 DKFLRDTILNFMIAGRDTLSSALSWFFFCLSNNPMVEAKIREELKGIINIPTNEAHD--- 360
           D+FLRDTIL+FM+AGRDT  SAL+WFF+ L NN     KIR+E+   +  P N+  D   
Sbjct: 301 DRFLRDTILSFMLAGRDTTGSALTWFFWLLCNNQEAMTKIRQEINTNL-FPRNKTDDGSV 360

Query: 361 --EWRIFSIEELKNLVYFHGALCETLRLYPPVPFQHKVPSQPDILPSGHHVNPKTKILFS 420
             +   F+ +E+K LVY HGA+CE LRLYPPVPF HK P++PD+LPSGH V   ++ILF 
Sbjct: 361 SYDSDSFNPQEVKKLVYLHGAVCEALRLYPPVPFNHKSPAKPDVLPSGHKVKANSRILFC 420

Query: 421 LYALGRTSDVWGKDCMEFKPERWISENGRIKHVPSYKFLAFNAGPRTCLGKEVAFLQMKI 480
           LY+LGR   VWG+D MEFKPERWISE+GR  H PSYKFL+FNAGPRTCLGKEVA  QMK 
Sbjct: 421 LYSLGRMKSVWGEDAMEFKPERWISESGRSVHEPSYKFLSFNAGPRTCLGKEVAMTQMKT 480

Query: 481 VAAAIIHNYNLQHIGRHKVVPSASIILHMKHGFEVMVTK 490
           VA  II NY++  +  HK+ P+ S+ILHMKHG +V V+K
Sbjct: 481 VAVKIIQNYDINVVEGHKIKPAPSVILHMKHGLKVTVSK 512

BLAST of Tan0001134 vs. TAIR 10
Match: AT4G32170.1 (cytochrome P450, family 96, subfamily A, polypeptide 2 )

HSP 1 Score: 455.3 bits (1170), Expect = 6.3e-128
Identity = 243/513 (47.37%), Postives = 330/513 (64.33%), Query Frame = 0

Query: 1   MASIAILSILLFSLLFIIFSVFVKWNPYG-LPWSWPLFGMIPTIVFHIHRIHDRVTEILK 60
           MASI++L + +  L F+I   F+   P+G  P +WP+  M+P ++  +HRI+D   +IL+
Sbjct: 1   MASISLLQVSVAILCFLILHYFLFKKPHGRYPRNWPVLRMLPAMLKALHRIYDYSVKILE 60

Query: 61  QAGSTFFFNGVWFADIDFLLTADPSNIHHIMSVNFERYPKGPDFKYIFDVLGDGIFNSDS 120
            +  TF F G  F  +D LLT DP+NIHHIMS NF  Y KGP+F+ +FDVLGD    +DS
Sbjct: 61  TSDLTFPFKGPRFTGMDMLLTVDPANIHHIMSSNFSNYIKGPEFQDVFDVLGDSFITTDS 120

Query: 121 DSWKNQRKTAHSL--------------------GLIPVLESVCENGSVLDLQDLFQRFSF 180
           + WKN RK+  ++                    GL+P+L    E G+ LDLQ +F RF+F
Sbjct: 121 ELWKNMRKSYQAMLHSQEFQRFSMSTMTSKLKYGLVPLLNHFAEEGTTLDLQSVFGRFTF 180

Query: 181 DSTCMLVTGFDLHSLSLEFPQVPFSKAMDDVEEVIFLRHFFPKKIWELQKKLQFGEAKRL 240
           D+  +LVTG D  SLS+E P+  F+KA+DDV E I  RHF P+ +W+LQ  + FG+ K+L
Sbjct: 181 DTIFILVTGSDPRSLSIEMPEDEFAKALDDVGEGILYRHFKPRFLWKLQNWIGFGQEKKL 240

Query: 241 KQAWEIIDQTIEKLLASKRESLKKLKEEPAEVGVGGVDLITSYI---TNKLEDEISNNND 300
            +A    D+   K +++KRE +K+          G  DL+TS+I   T K +  ++ ++D
Sbjct: 241 TEANATFDRVCAKYISAKREEIKR---SQGTSNGGSQDLLTSFIKLDTTKYK-LLNPSDD 300

Query: 301 KFLRDTILNFMIAGRDTLSSALSWFFFCLSNNPMVEAKIREELKGIINIPTNEAHDEWRI 360
           KFLRD IL F++AGRDT ++ALSWFF+ LS NP V AKI +E    INI T+ +      
Sbjct: 301 KFLRDNILAFILAGRDTTATALSWFFWLLSENPHVVAKIHQE----ININTDLSRTG--- 360

Query: 361 FSIEELKNLVYFHGALCETLRLYPPVPFQHKVPSQPDILPSGHHVNPKTKILFSLYALGR 420
            S E +  LVY HGALCE +RLYPPV F  K P + D+LPSGH V+  +KI+  LYALGR
Sbjct: 361 NSQENVDKLVYLHGALCEAMRLYPPVSFGRKSPIKSDVLPSGHKVDANSKIIICLYALGR 420

Query: 421 TSDVWGKDCMEFKPERWISENGRIKHVPSYKFLAFNAGPRTCLGKEVAFLQMKIVAAAII 480
              VWG+D  +FKPERWISENG IKH PS+KFL+FNAGPRTCLGK +A  QMKIVA  I+
Sbjct: 421 MRAVWGEDASQFKPERWISENGGIKHEPSFKFLSFNAGPRTCLGKHLAMTQMKIVAVEIL 480

Query: 481 HNYNLQHIGRHKVVPSASIILHMKHGFEVMVTK 490
            NY+++ +   K+VP+   IL MKHG ++ VTK
Sbjct: 481 RNYDIKVLQGQKIVPALGFILSMKHGLQITVTK 502

BLAST of Tan0001134 vs. TAIR 10
Match: AT1G65340.1 (cytochrome P450, family 96, subfamily A, polypeptide 3 )

HSP 1 Score: 451.1 bits (1159), Expect = 1.2e-126
Identity = 228/510 (44.71%), Postives = 322/510 (63.14%), Query Frame = 0

Query: 4   IAILSILLFSLLFIIFSVFV--KWNPYGLPWSWPLFGMIPTIVFHIHRIHDRVTEILKQA 63
           I +L I    + FI F   +  K  P  L  +WP  GM+P ++  + RI+D +TE+L+  
Sbjct: 5   IGLLEIFTAFVFFIFFQCLLLHKKTPKPLLTNWPALGMLPGLLLQVPRIYDWITEVLEAT 64

Query: 64  GSTFFFNGVWFADIDFLLTADPSNIHHIMSVNFERYPKGPDFKYIFDVLGDGIFNSDSDS 123
             TF F G   + +D LLT DP NIH+I+S NF  YPKG +FK IF+V+GD IFN DS  
Sbjct: 65  DMTFCFKGPCLSGMDILLTVDPVNIHYILSSNFANYPKGMEFKKIFEVVGDSIFNVDSGL 124

Query: 124 WKNQRKTAHSL--------------------GLIPVLESVCENGSVLDLQDLFQRFSFDS 183
           W++ R ++H++                    GL+P+LE+  +   ++DLQDLFQRF FD+
Sbjct: 125 WEDMRNSSHAIFSNQDFQMFWVSTSVRKLRQGLVPILENAADKNILVDLQDLFQRFLFDT 184

Query: 184 TCMLVTGFDLHSLSLEFPQVPFSKAMDDVEEVIFLRHFFPKKIWELQKKLQFGEAKRLKQ 243
           + +L+TG+D   LS+E P+V F  A+D V + +F RH  P  +W LQ  +  G  KRLK+
Sbjct: 185 SLILMTGYDPKCLSVEMPKVEFGDAVDGVSDGVFYRHVKPVFLWRLQYLIGVGVEKRLKR 244

Query: 244 AWEIIDQTIEKLLASKRESLKKLKEEPAEVGVGGVDLITSYIT--NKLEDEISNNNDKFL 303
              + DQ +EK++ +KRE +          G   +D++T Y+T        +  ++D+F+
Sbjct: 245 GLAVFDQLLEKIITAKREEINSHGTHHPSRG-EAIDVLTYYMTMDTTKYKYLEPSDDRFI 304

Query: 304 RDTILNFMIAGRDTLSSALSWFFFCLSNNPMVEAKIREELKGIINIPTNEAHDEWRIFSI 363
           +DTIL F+IA RDT SSAL+WFF+ +S NP    KIR+E+           + +   F  
Sbjct: 305 KDTILGFLIAARDTTSSALTWFFWLMSKNPEAINKIRQEV-----------NKKMPRFDP 364

Query: 364 EELKNLVYFHGALCETLRLYPPVPFQHKVPSQPDILPSGHHVNPKTKILFSLYALGRTSD 423
            +L+ LVY HGA+CETLRLYPPVPF HK P++PD+LPSGH V+ K KI+ S+YALGR   
Sbjct: 365 ADLEKLVYLHGAVCETLRLYPPVPFNHKSPAKPDVLPSGHRVDEKWKIVISMYALGRMKS 424

Query: 424 VWGKDCMEFKPERWISENGRIKHVPSYKFLAFNAGPRTCLGKEVAFLQMKIVAAAIIHNY 483
           VWG D  +F+PERWIS++GR+KH PSYKFLAFNAGPR CLGK++ FLQMK VAA II NY
Sbjct: 425 VWGDDAEDFRPERWISDSGRLKHEPSYKFLAFNAGPRACLGKKLTFLQMKTVAAEIIRNY 484

Query: 484 NLQHIGRHKVVPSASIILHMKHGFEVMVTK 490
           +++ +  HK  P  S++  M+HG +V +T+
Sbjct: 485 DIKVVEGHKTEPVPSVLFRMQHGLKVNITR 502

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FVS96.5e-12243.88Alkane hydroxylase MAH1 OS=Arabidopsis thaliana OX=3702 GN=CYP96A15 PE=2 SV=1[more]
A0A140IL905.5e-11341.73Noroxomaritidine synthase OS=Narcissus aff. pseudonarcissus MK-2014 OX=1540222 G... [more]
A0A140IL929.5e-11341.73Noroxomaritidine synthase 3 OS=Narcissus aff. pseudonarcissus MK-2014 OX=1540222... [more]
A0A140IL912.1e-11241.73Noroxomaritidine synthase 2 OS=Narcissus aff. pseudonarcissus MK-2014 OX=1540222... [more]
A0A2H5AJ003.6e-11241.54Noroxomaritidine synthase 1 OS=Narcissus pseudonarcissus OX=39639 GN=Cyp96T1 PE=... [more]
Match NameE-valueIdentityDescription
XP_038882620.14.8e-21574.32alkane hydroxylase MAH1-like isoform X1 [Benincasa hispida][more]
XP_038881122.15.4e-21472.41alkane hydroxylase MAH1-like [Benincasa hispida][more]
XP_022977942.12.0e-21372.80alkane hydroxylase MAH1-like [Cucurbita maxima][more]
XP_023544417.11.7e-21272.60alkane hydroxylase MAH1-like [Cucurbita pepo subsp. pepo][more]
XP_022950281.18.6e-21272.41alkane hydroxylase MAH1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1IJU09.9e-21472.80alkane hydroxylase MAH1-like OS=Cucurbita maxima OX=3661 GN=LOC111478084 PE=3 SV... [more]
A0A6J1GEE64.1e-21272.41alkane hydroxylase MAH1-like OS=Cucurbita moschata OX=3662 GN=LOC111453415 PE=3 ... [more]
A0A0A0KJ782.3e-21072.04Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G487530 PE=3 SV=1[more]
A0A6J1BWK73.0e-21072.96alkane hydroxylase MAH1-like OS=Momordica charantia OX=3673 GN=LOC111005984 PE=3... [more]
A0A6J1BTU45.1e-21073.44alkane hydroxylase MAH1-like OS=Momordica charantia OX=3673 GN=LOC111005299 PE=3... [more]
Match NameE-valueIdentityDescription
AT2G23180.16.7e-13849.02cytochrome P450, family 96, subfamily A, polypeptide 1 [more]
AT4G39490.11.1e-13748.28cytochrome P450, family 96, subfamily A, polypeptide 10 [more]
AT4G39480.17.4e-13747.78cytochrome P450, family 96, subfamily A, polypeptide 9 [more]
AT4G32170.16.3e-12847.37cytochrome P450, family 96, subfamily A, polypeptide 2 [more]
AT1G65340.11.2e-12644.71cytochrome P450, family 96, subfamily A, polypeptide 3 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 298..324
score: 30.33
coord: 438..461
score: 21.97
coord: 278..295
score: 29.8
coord: 428..438
score: 47.71
IPR001128Cytochrome P450PRINTSPR00385P450coord: 438..449
score: 34.09
coord: 289..306
score: 38.09
coord: 350..361
score: 38.96
coord: 429..438
score: 51.2
IPR001128Cytochrome P450PFAMPF00067p450coord: 31..468
e-value: 2.2E-58
score: 198.1
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 26..489
e-value: 5.6E-91
score: 307.5
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 22..489
NoneNo IPR availablePANTHERPTHR24296:SF193ALKANE HYDROXYLASE MAH1-LIKEcoord: 12..486
NoneNo IPR availablePANTHERPTHR24296CYTOCHROME P450coord: 12..486
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 431..440

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0001134.1Tan0001134.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen