Tan0000033 (gene) Snake gourd v1

Overview
NameTan0000033
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionheat stress transcription factor A-8
LocationLG04: 18703391 .. 18708149 (+)
RNA-Seq ExpressionTan0000033
SyntenyTan0000033
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CACTGACATGCCTCTCAAAAACCAAACAACACAACGCGGCAAAAAGGGATAAAACCCCCAAAATTCTTATTGTTCTACCAAAATTCTCAGAATCATTTTTCTATGTTTTCATTGCAATTTCTTCCTTTATCCTTTTGCTTTCCTCCTTATCACGCTACCTAATCTCATCGGTCTTTGAGGATTTGCAATGGTGAAATCCACGGCGGAGCGGCAGGGATCGTCGGTGGCGCCTTTTCTGAAGAAATTGTACGACATGGTCGACGATGAGGCCACCAATTCGATTATCTCCTGGAGTCCTACGGATGATAGCTTTACGATTTCGGATATGACCCAGTTCTCTATCCACTTGCTTCCTAAGTATTTTAAGCACAGCAATTTCTCTAGCTTTATGCGGCAGCTTAATATCTATGTGAGTTTCCTTGTCTGTTTGATTCTCTGTTGAAATTTTCAATTCGATGTTTGCGTTTCGTGTTTGTTTCTGTATTTTTGTGGTCTTGCTGATTTTTTCTCTCTTAAGAATGCAAGTCAGTCTAATAGTAGTACTTCATATATCTTGTTCATGAAGTCATCATTTGTTATGTTTGATTTCGGATTAAATGGTGAAGTTTCTCTATCATGAACTCGTCTTGTTTTAGTTTCCATAATTTCTAATAACGCAATTGAGGACTATATGGCTCATCAACTGGGAGGAGTTATATTCAAGATTGACTTTGAAAAAGCTTAGGAAAATGATGATGGGGGCTTGGTTGAAAGTGTGTTAGGTCGGTTAATTGCTCTGTTTTGGTTAATGGTAAGCTCAGGGGGAAATTTTTCCTCTAGACAGGGGGACTCTTAGCCCCTATCTTCTTCATCAAAATGCTCAATATTGTGTTATCATTAAAAACTTGGCAAATCCTATTTCTATATTTTTTTTTAAGTACCAAATAAAATATACCATTTATTAGAACCAGCTTGCTCAGGGCCGAGTAACTGGAGCTGCACAATACATCCTGACATAGTTACCATTATCCTCTTCTACAAGTGTAAGTTGCTTGTGGTTGGTATGGTCATTAGGGGTTAGCACTTGTGCCCAGGGTGGGGAAAGTTGTAGTATTTTAAAAGTTTCGTTTCATGTTAAAAAAAAATGGGTCGCTTATGTGAATTTAGTTGATAAAACTCTTCAACGTACTCACCTACTGTTCGATTTTGTTGGGGATATTTCTAGTTTTTTTTATTCAGTATTTGCTGGTAGAGGAAGAAACCTGACTTTGAGTAGATTTTTATCCTCAACTAGAAGTGACTGGGTTTTCCTTCCTTCATGTGTTCTATTCAGTTGTAATTGCTCACTAGGCTGCCGCTCCACCAATCTCTAAGCAACCAGCTTAATACATTTTTCTTTTTGTGTTCAAATTCTCATAAAATAACTTTCTACTGCTTGCACAGTCAGATAGTCTTCTACGTGATGGTGGCCATCAAAATATGGTAGATCAACATTTATCTTGTATTGATTGGCTGAACATGTGAGGAAAATTTTCTGGATCCCAAACTTGTTGCACTCCCCAAAGTATACATCTTCAATTTATTATTGTGTGTCACTCTTAGGTGCTGGTTCATGGTCGAGCCCCAAGGCTTTCCATACAAAGACAAACTAATCAGAAGTTCTATTTTGACTTTTGGCGTGCAAAGAGGAACTGATCAGATTTTGGTGATCAGAAGTGGAGGAGAATCATAAAAAAATAAAAAATTAATTAGGATGCTGAAGAGAAAGCGTTGAAATGTGCCAGTACATGTGAGTTTAATATTTGGCATTCTGAAATAGGGATGAAGAGAGAATTTAGTGGATTTGCAGGGGCCGTTAAATGTTTTGAGGTCGTCACAAAGGGAAGATTGGAAGAATTGGGCTTTTGAGCCTTCGGGATGATTCTCTTTTAAGTCACTGCTTTCCTTTTTTGTCAAAGGTCCTTTTGGATTCTCTTTTGTGCTCGATGGCCAGGATTCAGTGGTCATTTGGAGTGTCTGCTCTTATCTCGGGTTTATGGTTTGCACGAGATCAAAGGCTGTTTGAAGTCAAAGAAGTAGGGAAGCTAAAGTATTGGTCACGGTATTGTTAAAGAGAAGGGCGCCAGTTGAAGGATAATGGTTGTTTTCTGATGAGCAAGTTGGAAGAGCTTTGAAGATTGGTTTTTCTAGTGGTGGTGTTGGAGCCATTTCTCTCTTTTCATTATATCAATGAAATTCTCTTGTTACCTTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGATGAAGAGAGAAATGGCTCTATCTTGTCACTCTACTTGATATGGAGGCATGCCTTATGCAGCTCTTAGGTCCTTGAAGACCAATCGAACAAGTGAACTTTTATTCTTTTAAATTCATTTCCCTGCTCCTCTTGGGTTCATTTCTCGACAGGTTGATTATCGAGAGGCTGGGGTTCGGGTAAGGGAGAAATTTCACAAACATCTCACTTAATTCTAAATGTATATCTCCTTCGTCTGTAGCATTTTCTTTTACCCTTCTTGGAATATTCACTTCTTGTTTCATCGACTCTTGATTAAGGCATGATGCAATCTGCAGAAACAGGCTCCAAGTGCCATTTTCTTTCCTTTCTTTCTTCTATCCCTTTTTCTTTTTGGAATGGGAGTTTTCTTCCTGAGGCTATTATTCGTAGCTCAGTCAACCTTATTAGTTGTGATTCATAATACATGTTTTTAGGCCCTTGTGGAAAAGGGGAAATTGTTCAACATAGAGGCAAACTAGAGGAGAAACAACTAGATTTCCTCCTCTCTTCTGACATATAGTTTTGAATTGTCAAATCTTAGTTATAACAAGTGGTTCCTTGTATTATTTCAGGGTTTCCGGAAGATCGATACAGATTGCTGGGAATTTGCAACTGATGGATTTGTTAAAGGACAAAAACATCTGTTGAAGAACATCTACCGAAGGAAAAACATCCAGGCCACAGATCAACGTAAAGCGTCACAGCCCCAAGACAATTCCGAGGGACAAGGTGAACTACATGATTATACAGGCCTGTGGAAGGAAGTTGAGAATCTTAAAATAGATAAAAATGCTGTAATGCAGGAATTGGTTAAACTTAGGCAGCAGCAGGAAACTTCAGAGAATAAGTTGCTCCTATTGAGAGACCGCCTTCAAGGTATGGAAAAGAATCAACAGCAAATGCTTTCATTCTTAGTAATGGCAGTGCAAAGCCCTGGATTTTTGGTTCAGTTTCTTCAACCAAAAGAGAAGAATTGGCGGATGGCAGATGCTGGCAATATGCTGGAACAAATACCAGATGATAATCAAGTACCTTCAAATGGTATGATAGTCAGGTATCAACGTCCAATAGATGAACGTCCAGCAACTCTACTTCCACCGGTGTCGGGTCTGGGGAAACAACTAGAATCTGATCCATTCCCTGATGGAATGAAGGAATTTTTCCTTAATTCTGACTTCATGAAAATGCTTATGGATGAAAAGTTAGACAATCACAGTCAATTTGTTCTTCCAGATGTACAAGATGTCGCATGGGAGCAGCTTGCTAGCCCATTTTCATGGAATTTAGATAATGTTAGAAAAGTTGATGACGAAAGGGAACCTACGGACCATGATGACCACGAGGACCATGAACTGGATATGGAAACAATAGATACTCGAACTCATGAAGAGAACTCGCAAGATTTCGAACTTCTAATCAGACAAATGGAGAAAGGTGAAAATTTTGGGATGCAACCAGGGTTGGATGAAACTTATATCGAGAAATCAAATACTGTTAATCGTCTAGCCCAGCAGATGGAACTTTTGGCATCTGATCAAGAAATCTTATATGAAACACCAGAGAAAATGCAAGCCTATTAAAAGAATGATATGTTTTTGACGGGTTCATATTATTTCATCGATTTAAAATCTCAAAGATCAATATAAGTTTCTTTTATCTTCACTATCCACACTAATTAAAAACATTGGTTGGCTATGAAGTTATGGTGATGAGCTGCTGCAATTTCAATGTCTTGAGCAGAGAATTGAATTTTAAACAGGTTTATGGAATTTCAGCTATCTTTTTCATGGTGATACCTCAAGGTAATGCTTTTGTTTCAACTTTCAAGCATTATATCTTGTGGGCCTTGATAAATTTTTAATGATATATATATATATATATATATATGATCAATTTTGAAGAGGTGGTTTCTCAACTGCTTCTGGCTGACTTACTCCACATATTTTTCTGTATAGCTAGGCAGACACACTTGTTATACACTTGATATTAAATTTGATGTTGATTATCTCTCTCACACGATTTTGTTCATGTGCGCATATATCTTACACACAAGCAGAAAACTTTGTTGGTTATGTCATACCCAAATGATATTTGTTCATTTTTCAAATCTTGTAATGTTTTAGCCCTCGTACTTTAAATTTTAGTCCTTTTTCAAGTCCTTTAGTCAAATTTTTTTCTAAGAATCATGTATACTTGAAATATGGCAAGTGAATAAAAATCTGTCTGGACCTATGATTAAAGGTGGTTCAATTATTTATCTGTCTTTGCAATTTTGTGGTGCATTTGGATTGAGAAGAAAAATAAAATTTTTAGATAAGAAGTTTGGAGGTGGTTTGATAAAACACATTTGGAGGGTGTCCTTACTTTTAAGAGTCTCTTTCTAGGGTCATTACGAAATATGCATGCGTTTGAGACCATAAACTAACATCAATGAAAGCGAAATTCATTGAAAAATTTTAAACTACATAGG

mRNA sequence

CACTGACATGCCTCTCAAAAACCAAACAACACAACGCGGCAAAAAGGGATAAAACCCCCAAAATTCTTATTGTTCTACCAAAATTCTCAGAATCATTTTTCTATGTTTTCATTGCAATTTCTTCCTTTATCCTTTTGCTTTCCTCCTTATCACGCTACCTAATCTCATCGGTCTTTGAGGATTTGCAATGGTGAAATCCACGGCGGAGCGGCAGGGATCGTCGGTGGCGCCTTTTCTGAAGAAATTGTACGACATGGTCGACGATGAGGCCACCAATTCGATTATCTCCTGGAGTCCTACGGATGATAGCTTTACGATTTCGGATATGACCCAGTTCTCTATCCACTTGCTTCCTAAGTATTTTAAGCACAGCAATTTCTCTAGCTTTATGCGGCAGCTTAATATCTATGGTTTCCGGAAGATCGATACAGATTGCTGGGAATTTGCAACTGATGGATTTGTTAAAGGACAAAAACATCTGTTGAAGAACATCTACCGAAGGAAAAACATCCAGGCCACAGATCAACGTAAAGCGTCACAGCCCCAAGACAATTCCGAGGGACAAGGTGAACTACATGATTATACAGGCCTGTGGAAGGAAGTTGAGAATCTTAAAATAGATAAAAATGCTGTAATGCAGGAATTGGTTAAACTTAGGCAGCAGCAGGAAACTTCAGAGAATAAGTTGCTCCTATTGAGAGACCGCCTTCAAGGTATGGAAAAGAATCAACAGCAAATGCTTTCATTCTTAGTAATGGCAGTGCAAAGCCCTGGATTTTTGGTTCAGTTTCTTCAACCAAAAGAGAAGAATTGGCGGATGGCAGATGCTGGCAATATGCTGGAACAAATACCAGATGATAATCAAGTACCTTCAAATGGTATGATAGTCAGGTATCAACGTCCAATAGATGAACGTCCAGCAACTCTACTTCCACCGGTGTCGGGTCTGGGGAAACAACTAGAATCTGATCCATTCCCTGATGGAATGAAGGAATTTTTCCTTAATTCTGACTTCATGAAAATGCTTATGGATGAAAAGTTAGACAATCACAGTCAATTTGTTCTTCCAGATGTACAAGATGTCGCATGGGAGCAGCTTGCTAGCCCATTTTCATGGAATTTAGATAATGTTAGAAAAGTTGATGACGAAAGGGAACCTACGGACCATGATGACCACGAGGACCATGAACTGGATATGGAAACAATAGATACTCGAACTCATGAAGAGAACTCGCAAGATTTCGAACTTCTAATCAGACAAATGGAGAAAGGTGAAAATTTTGGGATGCAACCAGGGTTGGATGAAACTTATATCGAGAAATCAAATACTGTTAATCGTCTAGCCCAGCAGATGGAACTTTTGGCATCTGATCAAGAAATCTTATATGAAACACCAGAGAAAATGCAAGCCTATTAAAAGAATGATATGTTTTTGACGGGTTCATATTATTTCATCGATTTAAAATCTCAAAGATCAATATAAGTTTCTTTTATCTTCACTATCCACACTAATTAAAAACATTGGTTGGCTATGAAGTTATGGTGATGAGCTGCTGCAATTTCAATGTCTTGAGCAGAGAATTGAATTTTAAACAGGTTTATGGAATTTCAGCTATCTTTTTCATGGTGATACCTCAAGGTAATGCTTTTGTTTCAACTTTCAAGCATTATATCTTGTGGGCCTTGATAAATTTTTAATGATATATATATATATATATATATATGATCAATTTTGAAGAGGTGGTTTCTCAACTGCTTCTGGCTGACTTACTCCACATATTTTTCTGTATAGCTAGGCAGACACACTTGTTATACACTTGATATTAAATTTGATGTTGATTATCTCTCTCACACGATTTTGTTCATGTGCGCATATATCTTACACACAAGCAGAAAACTTTGTTGGTTATGTCATACCCAAATGATATTTGTTCATTTTTCAAATCTTGTAATGTTTTAGCCCTCGTACTTTAAATTTTAGTCCTTTTTCAAGTCCTTTAGTCAAATTTTTTTCTAAGAATCATGTATACTTGAAATATGGCAAGTGAATAAAAATCTGTCTGGACCTATGATTAAAGGTGGTTCAATTATTTATCTGTCTTTGCAATTTTGTGGTGCATTTGGATTGAGAAGAAAAATAAAATTTTTAGATAAGAAGTTTGGAGGTGGTTTGATAAAACACATTTGGAGGGTGTCCTTACTTTTAAGAGTCTCTTTCTAGGGTCATTACGAAATATGCATGCGTTTGAGACCATAAACTAACATCAATGAAAGCGAAATTCATTGAAAAATTTTAAACTACATAGG

Coding sequence (CDS)

ATGGTGAAATCCACGGCGGAGCGGCAGGGATCGTCGGTGGCGCCTTTTCTGAAGAAATTGTACGACATGGTCGACGATGAGGCCACCAATTCGATTATCTCCTGGAGTCCTACGGATGATAGCTTTACGATTTCGGATATGACCCAGTTCTCTATCCACTTGCTTCCTAAGTATTTTAAGCACAGCAATTTCTCTAGCTTTATGCGGCAGCTTAATATCTATGGTTTCCGGAAGATCGATACAGATTGCTGGGAATTTGCAACTGATGGATTTGTTAAAGGACAAAAACATCTGTTGAAGAACATCTACCGAAGGAAAAACATCCAGGCCACAGATCAACGTAAAGCGTCACAGCCCCAAGACAATTCCGAGGGACAAGGTGAACTACATGATTATACAGGCCTGTGGAAGGAAGTTGAGAATCTTAAAATAGATAAAAATGCTGTAATGCAGGAATTGGTTAAACTTAGGCAGCAGCAGGAAACTTCAGAGAATAAGTTGCTCCTATTGAGAGACCGCCTTCAAGGTATGGAAAAGAATCAACAGCAAATGCTTTCATTCTTAGTAATGGCAGTGCAAAGCCCTGGATTTTTGGTTCAGTTTCTTCAACCAAAAGAGAAGAATTGGCGGATGGCAGATGCTGGCAATATGCTGGAACAAATACCAGATGATAATCAAGTACCTTCAAATGGTATGATAGTCAGGTATCAACGTCCAATAGATGAACGTCCAGCAACTCTACTTCCACCGGTGTCGGGTCTGGGGAAACAACTAGAATCTGATCCATTCCCTGATGGAATGAAGGAATTTTTCCTTAATTCTGACTTCATGAAAATGCTTATGGATGAAAAGTTAGACAATCACAGTCAATTTGTTCTTCCAGATGTACAAGATGTCGCATGGGAGCAGCTTGCTAGCCCATTTTCATGGAATTTAGATAATGTTAGAAAAGTTGATGACGAAAGGGAACCTACGGACCATGATGACCACGAGGACCATGAACTGGATATGGAAACAATAGATACTCGAACTCATGAAGAGAACTCGCAAGATTTCGAACTTCTAATCAGACAAATGGAGAAAGGTGAAAATTTTGGGATGCAACCAGGGTTGGATGAAACTTATATCGAGAAATCAAATACTGTTAATCGTCTAGCCCAGCAGATGGAACTTTTGGCATCTGATCAAGAAATCTTATATGAAACACCAGAGAAAATGCAAGCCTATTAA

Protein sequence

MVKSTAERQGSSVAPFLKKLYDMVDDEATNSIISWSPTDDSFTISDMTQFSIHLLPKYFKHSNFSSFMRQLNIYGFRKIDTDCWEFATDGFVKGQKHLLKNIYRRKNIQATDQRKASQPQDNSEGQGELHDYTGLWKEVENLKIDKNAVMQELVKLRQQQETSENKLLLLRDRLQGMEKNQQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGMIVRYQRPIDERPATLLPPVSGLGKQLESDPFPDGMKEFFLNSDFMKMLMDEKLDNHSQFVLPDVQDVAWEQLASPFSWNLDNVRKVDDEREPTDHDDHEDHELDMETIDTRTHEENSQDFELLIRQMEKGENFGMQPGLDETYIEKSNTVNRLAQQMELLASDQEILYETPEKMQAY
Homology
BLAST of Tan0000033 vs. ExPASy Swiss-Prot
Match: Q9S7U5 (Heat stress transcription factor A-8 OS=Arabidopsis thaliana OX=3702 GN=HSFA8 PE=2 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 3.1e-77
Identity = 186/405 (45.93%), Postives = 250/405 (61.73%), Query Frame = 0

Query: 1   MVKST----AERQGSSVAPFLKKLYDMVDDEATNSIISWSPT-DDSFTISDMTQFSIHLL 60
           MVKST         SSVAPFL+K YDMVDD  T+SIISWSP+ D+SF I D T FS+ LL
Sbjct: 1   MVKSTDGGGGSSSSSSVAPFLRKCYDMVDDSTTDSIISWSPSADNSFVILDTTVFSVQLL 60

Query: 61  PKYFKHSNFSSFMRQLNIYGFRKIDTDCWEFATDGFVKGQKHLLKNIYRRKNIQATDQRK 120
           PKYFKHSNFSSF+RQLNIYGFRK+D D WEFA DGFV+GQK LLKN+ RRKN+Q+++Q K
Sbjct: 61  PKYFKHSNFSSFIRQLNIYGFRKVDADRWEFANDGFVRGQKDLLKNVIRRKNVQSSEQSK 120

Query: 121 ASQPQDNSEGQGELHDYTGLWKEVENLKIDKNAVMQELVKLRQQQETSENKLLLLRDRLQ 180
                  S       + +GLWKEV+ LK DK  + QEL+K+RQ QE ++ K+L L DR+Q
Sbjct: 121 -----HESTSTTYAQEKSGLWKEVDILKGDKQVLAQELIKVRQYQEVTDTKMLHLEDRVQ 180

Query: 181 GMEKNQQQMLSFLVMAVQSPGFLVQFLQPKEKN-WRMADAG-NMLEQIPDDNQVPSNGM- 240
           GME++QQ+MLSFLVM +++P  LVQ LQPKEKN WR A  G  ++E++ D+ +  S G+ 
Sbjct: 181 GMEESQQEMLSFLVMVMKNPSLLVQLLQPKEKNTWRKAGEGAKIVEEVTDEGESNSYGLP 240

Query: 241 IVRYQRPIDERPATLLPPVSGLGKQLESDPFPDGMKEFFLNSDFMKMLMDEKLDNHSQFV 300
           +V YQ P D          +G  K   +D     + +F  N+D +K  +DE   NH   +
Sbjct: 241 LVTYQPPSDN---------NGTAKSNSND-----VNDFLRNADMLKFCLDE---NHVPLI 300

Query: 301 LPDV-QDVAWEQLASPFSWNLDNVRKVDDEREPTDHDDHEDHELDMETIDTRTHEENSQD 360
           +PD+  D AWE+L       L   RK   ++E       +D  L+ E  D     + S  
Sbjct: 301 IPDLYDDGAWEKLLL-----LSPSRKKTKKQENIVKKGKDDLTLEEEEEDGTMELDKSYM 360

Query: 361 FELLIRQMEKGENFGMQPGLDETYIEKSNTVNRLAQQMELLASDQ 397
            +L+  +MEK ++F       +   E+S  +  L +QMELLAS++
Sbjct: 361 LKLISEEMEKPDDF----EFGQLTPERSRNLEILTEQMELLASNE 374

BLAST of Tan0000033 vs. ExPASy Swiss-Prot
Match: Q40152 (Heat shock factor protein HSF8 OS=Solanum lycopersicum OX=4081 GN=HSF8 PE=3 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 5.5e-58
Identity = 130/276 (47.10%), Postives = 176/276 (63.77%), Query Frame = 0

Query: 15  PFLKKLYDMVDDEATNSIISWSPTDDSFTISDMTQFSIHLLPKYFKHSNFSSFMRQLNIY 74
           PFL K YDMVDD +T+ I+SWSPT++SF + D  +F+  LLPKYFKH+NFSSF+RQLN Y
Sbjct: 39  PFLVKTYDMVDDPSTDKIVSWSPTNNSFVVWDPPEFAKDLLPKYFKHNNFSSFVRQLNTY 98

Query: 75  GFRKIDTDCWEFATDGFVKGQKHLLKNIYRRKNIQATDQRKASQPQDNSEGQGELHDYT- 134
           GFRK+D D WEFA +GF++GQKHLLK+I RRK      Q++  QP  N++ Q +   ++ 
Sbjct: 99  GFRKVDPDRWEFANEGFLRGQKHLLKSISRRKPAHGHAQQQ-QQPHGNAQQQMQPPGHSA 158

Query: 135 -----------GLWKEVENLKIDKNAVMQELVKLRQQQETSENKLLLLRDRLQGMEKNQQ 194
                      GL +EVE LK DKN +MQELV+LRQQQ+ ++N+L  +  RLQGME  QQ
Sbjct: 159 SVGACVEVGKFGLEEEVERLKRDKNVLMQELVRLRQQQQATDNQLQGMVQRLQGMELRQQ 218

Query: 195 QMLSFLVMAVQSPGFLVQFLQPK-EKNWRMADAGNMLE-----QIPDDNQVPSNGMIVRY 254
           QM+SFL  AV  PGFL QF+Q + E N R+A+           +  D +  P++G IV+Y
Sbjct: 219 QMMSFLAKAVNRPGFLAQFVQQQNESNKRIAEGSKKRRIKQDIESQDPSVTPADGQIVKY 278

Query: 255 QRPIDERPATLLPPVSGLGKQLESDPFPDGMKEFFL 273
           Q  I+E    +L  +S L      D F +  + F +
Sbjct: 279 QPGINEAAKAMLRELSKLDSSPRLDNFSNSPESFLI 313

BLAST of Tan0000033 vs. ExPASy Swiss-Prot
Match: P41153 (Heat shock factor protein HSF8 OS=Solanum peruvianum OX=4082 GN=HSF8 PE=2 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 1.2e-57
Identity = 129/276 (46.74%), Postives = 178/276 (64.49%), Query Frame = 0

Query: 15  PFLKKLYDMVDDEATNSIISWSPTDDSFTISDMTQFSIHLLPKYFKHSNFSSFMRQLNIY 74
           PFL K YDMVDD +T+ I+SWSPT++SF + D  +F+  LLPKYFKH+NFSSF+RQLN Y
Sbjct: 41  PFLVKTYDMVDDPSTDKIVSWSPTNNSFVVWDPPEFAKDLLPKYFKHNNFSSFVRQLNTY 100

Query: 75  GFRKIDTDCWEFATDGFVKGQKHLLKNIYRRKNIQATDQRKASQPQDNSEGQGELHDYT- 134
           GFRK+D D WEFA +GF++GQKHLLK+I RRK      Q++  QP  +++ Q +   ++ 
Sbjct: 101 GFRKVDPDRWEFANEGFLRGQKHLLKSISRRKPAHGHAQQQ-QQPHGHAQQQMQPPGHSA 160

Query: 135 -----------GLWKEVENLKIDKNAVMQELVKLRQQQETSENKLLLLRDRLQGMEKNQQ 194
                      GL +EVE LK DKN +MQELV+LRQQQ++++N+L  +  RLQGME  QQ
Sbjct: 161 SVGACVEVGKFGLEEEVERLKRDKNVLMQELVRLRQQQQSTDNQLQGMVQRLQGMELRQQ 220

Query: 195 QMLSFLVMAVQSPGFLVQFLQPK-EKNWRMADAGNMLE-----QIPDDNQVPSNGMIVRY 254
           QM+SFL  AV SPGFL QF+Q + E N R+A+           +  D +  P++G IV+Y
Sbjct: 221 QMMSFLAKAVNSPGFLAQFVQQQNESNKRIAEGSKKRRIKQDIESQDPSVTPADGQIVKY 280

Query: 255 QRPIDERPATLLPPVSGLGKQLESDPFPDGMKEFFL 273
           Q  I+E    +L  +S L      + F +  + F +
Sbjct: 281 QPGINEAAKAMLRELSKLDSSPRLENFSNSPESFLI 315

BLAST of Tan0000033 vs. ExPASy Swiss-Prot
Match: P41151 (Heat stress transcription factor A-1a OS=Arabidopsis thaliana OX=3702 GN=HSFA1A PE=1 SV=2)

HSP 1 Score: 219.2 bits (557), Expect = 8.8e-56
Identity = 123/244 (50.41%), Postives = 163/244 (66.80%), Query Frame = 0

Query: 15  PFLKKLYDMVDDEATNSIISWSPTDDSFTISDMTQFSIHLLPKYFKHSNFSSFMRQLNIY 74
           PFL K YDMV+D AT++I+SWSPT++SF + D  +FS  LLPKYFKH+NFSSF+RQLN Y
Sbjct: 52  PFLSKTYDMVEDPATDAIVSWSPTNNSFIVWDPPEFSRDLLPKYFKHNNFSSFVRQLNTY 111

Query: 75  GFRKIDTDCWEFATDGFVKGQKHLLKNIYRRKNIQATDQRKAS-QPQDNSEGQGELHDYT 134
           GFRK+D D WEFA +GF++GQKHLLK I RRK++Q      ++ Q Q  S+GQG +   +
Sbjct: 112 GFRKVDPDRWEFANEGFLRGQKHLLKKISRRKSVQGHGSSSSNPQSQQLSQGQGSMAALS 171

Query: 135 --------GLWKEVENLKIDKNAVMQELVKLRQQQETSENKLLLLRDRLQGMEKNQQQML 194
                   GL +EVE LK DKN +MQELVKLRQQQ+T++NKL +L   LQ ME+ QQQ++
Sbjct: 172 SCVEVGKFGLEEEVEQLKRDKNVLMQELVKLRQQQQTTDNKLQVLVKHLQVMEQRQQQIM 231

Query: 195 SFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQV------------PSNGMI 238
           SFL  AVQ+P FL QF+Q +  +       N   ++ +D+               S+G I
Sbjct: 232 SFLAKAVQNPTFLSQFIQKQTDSNMHVTEANKKRRLREDSTAATESNSHSHSLEASDGQI 291

BLAST of Tan0000033 vs. ExPASy Swiss-Prot
Match: Q84T61 (Heat stress transcription factor A-1 OS=Oryza sativa subsp. japonica OX=39947 GN=HSFA1 PE=2 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 2.0e-55
Identity = 125/270 (46.30%), Postives = 171/270 (63.33%), Query Frame = 0

Query: 15  PFLKKLYDMVDDEATNSIISWSPTDDSFTISDMTQFSIHLLPKYFKHSNFSSFMRQLNIY 74
           PFL K Y+MVDD AT++++SW P ++SF + +  +F+  LLPKYFKHSNFSSF+RQLN Y
Sbjct: 37  PFLMKTYEMVDDPATDAVVSWGPGNNSFVVWNTPEFARDLLPKYFKHSNFSSFVRQLNTY 96

Query: 75  GFRKIDTDCWEFATDGFVKGQKHLLKNIYRRKNIQATDQRKASQ-PQDNSEGQGELHDYT 134
           GFRK+D D WEFA +GF++GQKHLLK I RRK     +Q +  Q P        E+  + 
Sbjct: 97  GFRKVDPDRWEFANEGFLRGQKHLLKTINRRKPTHGNNQVQQPQLPAAPVPACVEVGKF- 156

Query: 135 GLWKEVENLKIDKNAVMQELVKLRQQQETSENKLLLLRDRLQGMEKNQQQMLSFLVMAVQ 194
           G+ +E+E LK DKN +MQELV+LRQQQ+T++++L  L  RLQGME+ QQQM+SFL  A+ 
Sbjct: 157 GMEEEIEMLKRDKNVLMQELVRLRQQQQTTDHQLQTLGKRLQGMEQRQQQMMSFLAKAMH 216

Query: 195 SPGFLVQFLQPKEKNWRMADAGNMLEQIP------DDNQVPSNGMIVRYQRPIDERPATL 254
           SPGFL QF+Q  E + R   A N   ++P      D      +G IV+YQ  I+E    +
Sbjct: 217 SPGFLAQFVQQNENSRRRIVASNKKRRLPKQDGSLDSESASLDGQIVKYQPMINEAAKAM 276

Query: 255 LPPVSGLGKQLESDPFPDGMKEFFLNSDFM 278
           L  +  L      +    G  + FL  ++M
Sbjct: 277 LRKILKLDSSHRFESM--GNSDNFLLENYM 303

BLAST of Tan0000033 vs. NCBI nr
Match: KAG6599929.1 (Heat stress transcription factor A-8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 724.2 bits (1868), Expect = 6.4e-205
Identity = 371/411 (90.27%), Postives = 384/411 (93.43%), Query Frame = 0

Query: 1   MVKSTAERQGSSVAPFLKKLYDMVDDEATNSIISWSPTDDSFTISDMTQFSIHLLPKYFK 60
           MVKS  ERQGSSVAPFLKKLY+MVDDEATNSIISWSP DDSFTI DMT FS+HLLPKYFK
Sbjct: 1   MVKSAGERQGSSVAPFLKKLYEMVDDEATNSIISWSPRDDSFTILDMTHFSVHLLPKYFK 60

Query: 61  HSNFSSFMRQLNIYGFRKIDTDCWEFATDGFVKGQKHLLKNIYRRKNIQATDQRKASQPQ 120
           HSNFSSFMRQLNIYGFRKI+TDCWEFATDGFVKGQKHLLKNIYRRKNIQ TDQRKASQPQ
Sbjct: 61  HSNFSSFMRQLNIYGFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQRKASQPQ 120

Query: 121 DNSEGQGELHDYTGLWKEVENLKIDKNAVMQELVKLRQQQETSENKLLLLRDRLQGMEKN 180
           DNSEGQGELHDYTGLWKEVENLKIDKNAVMQELVKLRQQQETSENK+LLLRDRLQGMEKN
Sbjct: 121 DNSEGQGELHDYTGLWKEVENLKIDKNAVMQELVKLRQQQETSENKMLLLRDRLQGMEKN 180

Query: 181 QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGMIVRYQRPI 240
           QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNG+IVRYQRPI
Sbjct: 181 QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGLIVRYQRPI 240

Query: 241 DERPATLLPPVSGLGKQLESDPFPDGMKEFFLNSDFMKMLMDEKLDNHSQFVLPDVQDVA 300
           DE PA LLPPVSGLGKQ ESDPFPDGMK+FFLNSDFMK+LMDEKLDNHSQFVLPDVQDVA
Sbjct: 241 DELPAPLLPPVSGLGKQQESDPFPDGMKDFFLNSDFMKVLMDEKLDNHSQFVLPDVQDVA 300

Query: 301 WEQ--LASPFSWNLDNVRKVDDEREPTDHDDHEDHELDMETIDTRTHEENSQDFELLIRQ 360
           WEQ  LAS  S N DNVRKVDDEREP DH+D EDHELDMETIDTRTHEEN QDFELLI+Q
Sbjct: 301 WEQLLLASTVSCNSDNVRKVDDEREPMDHEDLEDHELDMETIDTRTHEENLQDFELLIKQ 360

Query: 361 MEKGENFGMQPGLDETYIEKSNTVNRLAQQMELLASDQEILYETPEKMQAY 410
           MEKGENF +QP LDE+YIEKSNTVN     MEL+ASDQEILYETP KMQ +
Sbjct: 361 MEKGENFRLQPRLDESYIEKSNTVN----LMELMASDQEILYETPAKMQGH 407

BLAST of Tan0000033 vs. NCBI nr
Match: XP_022942461.1 (heat stress transcription factor A-8-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 721.8 bits (1862), Expect = 3.2e-204
Identity = 371/411 (90.27%), Postives = 383/411 (93.19%), Query Frame = 0

Query: 1   MVKSTAERQGSSVAPFLKKLYDMVDDEATNSIISWSPTDDSFTISDMTQFSIHLLPKYFK 60
           MVKS  ERQGSSVAPFLKKLY+MVDDEATNSIISWSP DDSFTI DMT FS+HLLPKYFK
Sbjct: 1   MVKSAGERQGSSVAPFLKKLYEMVDDEATNSIISWSPRDDSFTILDMTHFSVHLLPKYFK 60

Query: 61  HSNFSSFMRQLNIYGFRKIDTDCWEFATDGFVKGQKHLLKNIYRRKNIQATDQRKASQPQ 120
           HSNFSSFMRQLNIYGFRKI+TDCWEFATDGFVKGQKHLLKNIYRRKNIQ TDQRKASQPQ
Sbjct: 61  HSNFSSFMRQLNIYGFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQRKASQPQ 120

Query: 121 DNSEGQGELHDYTGLWKEVENLKIDKNAVMQELVKLRQQQETSENKLLLLRDRLQGMEKN 180
           DNSEGQGELHDYTGLWKEVENLKIDKNAVMQELVKLRQQQETSENK+LLLRDRLQGMEKN
Sbjct: 121 DNSEGQGELHDYTGLWKEVENLKIDKNAVMQELVKLRQQQETSENKMLLLRDRLQGMEKN 180

Query: 181 QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGMIVRYQRPI 240
           QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNG+IVRYQRPI
Sbjct: 181 QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGLIVRYQRPI 240

Query: 241 DERPATLLPPVSGLGKQLESDPFPDGMKEFFLNSDFMKMLMDEKLDNHSQFVLPDVQDVA 300
           DE PA LLPPVSGLGKQ ESDPFPDGMK+FFLNSDFMK+LMDEKLDNHSQFVLPDVQDVA
Sbjct: 241 DELPAPLLPPVSGLGKQQESDPFPDGMKDFFLNSDFMKVLMDEKLDNHSQFVLPDVQDVA 300

Query: 301 WEQ--LASPFSWNLDNVRKVDDEREPTDHDDHEDHELDMETIDTRTHEENSQDFELLIRQ 360
           WEQ  LAS  S N DNVRKVDDEREP    DHEDHELDMETIDTRTHEEN QDFELLI+Q
Sbjct: 301 WEQLLLASTVSCNSDNVRKVDDEREPM---DHEDHELDMETIDTRTHEENLQDFELLIKQ 360

Query: 361 MEKGENFGMQPGLDETYIEKSNTVNRLAQQMELLASDQEILYETPEKMQAY 410
           MEKGENFG+QP LDE+YIEKSNTVN     MEL+ASDQEILYETP KMQ +
Sbjct: 361 MEKGENFGLQPRLDESYIEKSNTVN----LMELMASDQEILYETPAKMQGH 404

BLAST of Tan0000033 vs. NCBI nr
Match: XP_023536181.1 (heat stress transcription factor A-8-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 718.8 bits (1854), Expect = 2.7e-203
Identity = 370/411 (90.02%), Postives = 382/411 (92.94%), Query Frame = 0

Query: 1   MVKSTAERQGSSVAPFLKKLYDMVDDEATNSIISWSPTDDSFTISDMTQFSIHLLPKYFK 60
           MVKS  ERQGSSVAPFLKKLY+MVDDEATNSIISWSP DDSFTI DMT FS+HLLPKYFK
Sbjct: 1   MVKSAGERQGSSVAPFLKKLYEMVDDEATNSIISWSPRDDSFTILDMTHFSVHLLPKYFK 60

Query: 61  HSNFSSFMRQLNIYGFRKIDTDCWEFATDGFVKGQKHLLKNIYRRKNIQATDQRKASQPQ 120
           HSNFSSFMRQLNIYGFRKI+TDCWEFATDGFVKGQKHLLKNIYRRKNIQ TDQRKASQPQ
Sbjct: 61  HSNFSSFMRQLNIYGFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQRKASQPQ 120

Query: 121 DNSEGQGELHDYTGLWKEVENLKIDKNAVMQELVKLRQQQETSENKLLLLRDRLQGMEKN 180
           DNSEGQGELHDYTGLWKEVENLKIDKNAVMQELVKLRQQQETSENK+LLLRDRLQGMEKN
Sbjct: 121 DNSEGQGELHDYTGLWKEVENLKIDKNAVMQELVKLRQQQETSENKMLLLRDRLQGMEKN 180

Query: 181 QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGMIVRYQRPI 240
           QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNG+IVRYQRPI
Sbjct: 181 QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGLIVRYQRPI 240

Query: 241 DERPATLLPPVSGLGKQLESDPFPDGMKEFFLNSDFMKMLMDEKLDNHSQFVLPDVQDVA 300
           DE PA LLPPVSGLGKQ ESDPFPDGMK+FFLNSDFMK+LMDEKLDNHSQFVLPDVQDVA
Sbjct: 241 DELPAPLLPPVSGLGKQQESDPFPDGMKDFFLNSDFMKVLMDEKLDNHSQFVLPDVQDVA 300

Query: 301 WEQ--LASPFSWNLDNVRKVDDEREPTDHDDHEDHELDMETIDTRTHEENSQDFELLIRQ 360
           WEQ  LAS  S N DNVRKVDDEREP    DHEDHELDMETIDTRTHEEN QDFELLI+Q
Sbjct: 301 WEQLLLASTVSCNSDNVRKVDDEREPM---DHEDHELDMETIDTRTHEENLQDFELLIKQ 360

Query: 361 MEKGENFGMQPGLDETYIEKSNTVNRLAQQMELLASDQEILYETPEKMQAY 410
           MEKGENFG+QP LDE+YIEKSNTVN     MEL+ASDQEILYET  KMQ +
Sbjct: 361 MEKGENFGLQPRLDESYIEKSNTVN----LMELMASDQEILYETQPKMQGH 404

BLAST of Tan0000033 vs. NCBI nr
Match: XP_022995879.1 (heat stress transcription factor A-8-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 717.2 bits (1850), Expect = 7.9e-203
Identity = 368/411 (89.54%), Postives = 382/411 (92.94%), Query Frame = 0

Query: 1   MVKSTAERQGSSVAPFLKKLYDMVDDEATNSIISWSPTDDSFTISDMTQFSIHLLPKYFK 60
           MVKS  ERQGSSVAPFLKKLY+MVDDEATNSIISWSP DDSFTI DMT FS+HLLPKYFK
Sbjct: 1   MVKSAGERQGSSVAPFLKKLYEMVDDEATNSIISWSPRDDSFTILDMTHFSVHLLPKYFK 60

Query: 61  HSNFSSFMRQLNIYGFRKIDTDCWEFATDGFVKGQKHLLKNIYRRKNIQATDQRKASQPQ 120
           HSNFSSFMRQLNIYGFRKI+TDCWEFATDGFVKGQKHLLKNIYRRKNIQ TDQR+ASQPQ
Sbjct: 61  HSNFSSFMRQLNIYGFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQRRASQPQ 120

Query: 121 DNSEGQGELHDYTGLWKEVENLKIDKNAVMQELVKLRQQQETSENKLLLLRDRLQGMEKN 180
           DNSEGQGELHDYTGLWKEVENLKIDKN VMQELVKLRQQQETSE+K+LLLRDRLQGMEKN
Sbjct: 121 DNSEGQGELHDYTGLWKEVENLKIDKNGVMQELVKLRQQQETSESKMLLLRDRLQGMEKN 180

Query: 181 QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGMIVRYQRPI 240
           QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNG+IVRYQRPI
Sbjct: 181 QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGLIVRYQRPI 240

Query: 241 DERPATLLPPVSGLGKQLESDPFPDGMKEFFLNSDFMKMLMDEKLDNHSQFVLPDVQDVA 300
           DE PA LLPPVSGLGKQ ESDPFPDGMK+FFLNSDFMK+LMDEKLDNHSQFVLPDVQDVA
Sbjct: 241 DELPAPLLPPVSGLGKQQESDPFPDGMKDFFLNSDFMKVLMDEKLDNHSQFVLPDVQDVA 300

Query: 301 WEQ--LASPFSWNLDNVRKVDDEREPTDHDDHEDHELDMETIDTRTHEENSQDFELLIRQ 360
           WEQ  LAS  S N DNVRKVDDEREP    DHEDHELDMETIDTRTHEEN QDFELLI+Q
Sbjct: 301 WEQLLLASTVSCNSDNVRKVDDEREPM---DHEDHELDMETIDTRTHEENLQDFELLIKQ 360

Query: 361 MEKGENFGMQPGLDETYIEKSNTVNRLAQQMELLASDQEILYETPEKMQAY 410
           MEKGENFG+QP LDE+YIEKSNTVN     MEL+ASDQEILYETP KMQ +
Sbjct: 361 MEKGENFGLQPRLDESYIEKSNTVN----LMELMASDQEILYETPAKMQGH 404

BLAST of Tan0000033 vs. NCBI nr
Match: XP_038892281.1 (heat stress transcription factor A-8 [Benincasa hispida])

HSP 1 Score: 672.9 bits (1735), Expect = 1.7e-189
Identity = 350/411 (85.16%), Postives = 373/411 (90.75%), Query Frame = 0

Query: 1   MVKSTAERQGSSVAPFLKKLYDMVDDEATNSIISWSPTDDSFTISDMTQFSIHLLPKYFK 60
           MVKS AERQGSSVAPFLKKLYDMVDD++TNSIISW+ + DSFTI D+TQFS+H+LPKYFK
Sbjct: 1   MVKSAAERQGSSVAPFLKKLYDMVDDDSTNSIISWTSSHDSFTILDITQFSLHVLPKYFK 60

Query: 61  HSNFSSFMRQLNIYGFRKIDTDCWEFATDGFVKGQKHLLKNIYRRKNIQATDQRKASQPQ 120
           HSNFSSFMRQLNIYGFRKIDTDCWEFATDGF+KGQKHLLKNIYRRKNIQ TDQR+ASQPQ
Sbjct: 61  HSNFSSFMRQLNIYGFRKIDTDCWEFATDGFIKGQKHLLKNIYRRKNIQGTDQRRASQPQ 120

Query: 121 DNSEGQGELHDYTGLWKEVENLKIDKNAVMQELVKLRQQQETSENKLLLLRDRLQGMEKN 180
           DNS+GQ EL DY+GLWKEVENLKIDKNAVMQELVKL+Q QETSENKLLLLRDRLQGMEKN
Sbjct: 121 DNSDGQVELPDYSGLWKEVENLKIDKNAVMQELVKLKQHQETSENKLLLLRDRLQGMEKN 180

Query: 181 QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGMIVRYQRPI 240
           QQQMLSFLVMAVQSPGFLVQ LQPKEKNWRMAD GNMLEQIPDDNQVPSNGMIVRYQRP+
Sbjct: 181 QQQMLSFLVMAVQSPGFLVQLLQPKEKNWRMADTGNMLEQIPDDNQVPSNGMIVRYQRPL 240

Query: 241 DERPATLLPPVSGLGKQLESDPFPDGMKEFFLNSDFMKMLMDEK--LDNHSQFVLPDVQD 300
           DE   TLLP V+G GKQ ESDPFPDGMK+FFLNSDFMK+LMDEK  LDNHSQFVLPDVQD
Sbjct: 241 DELSTTLLPAVTGPGKQQESDPFPDGMKDFFLNSDFMKVLMDEKLCLDNHSQFVLPDVQD 300

Query: 301 VAWEQ--LASPFSWNLDNVRKVDDEREPTDHDDHEDHELDMETIDTRTHEENSQDFELLI 360
           VAWEQ  LASPFS N DN RK+DDER    H D ED ELDMETIDT+THEENSQDFELLI
Sbjct: 301 VAWEQLLLASPFSGNSDNGRKIDDERR---HMDDEDDELDMETIDTQTHEENSQDFELLI 360

Query: 361 RQMEKGENFGMQPGLDETYIEKSNTVNRLAQQMELLASDQEILYETPEKMQ 408
           RQMEK E+F +QP LDE+YIEK NTVN L QQME LASDQEILYET +K+Q
Sbjct: 361 RQMEKCEDFEIQPRLDESYIEKLNTVNLLTQQMEPLASDQEILYETADKIQ 408

BLAST of Tan0000033 vs. ExPASy TrEMBL
Match: A0A6J1FNX4 (heat stress transcription factor A-8-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111447493 PE=3 SV=1)

HSP 1 Score: 721.8 bits (1862), Expect = 1.5e-204
Identity = 371/411 (90.27%), Postives = 383/411 (93.19%), Query Frame = 0

Query: 1   MVKSTAERQGSSVAPFLKKLYDMVDDEATNSIISWSPTDDSFTISDMTQFSIHLLPKYFK 60
           MVKS  ERQGSSVAPFLKKLY+MVDDEATNSIISWSP DDSFTI DMT FS+HLLPKYFK
Sbjct: 1   MVKSAGERQGSSVAPFLKKLYEMVDDEATNSIISWSPRDDSFTILDMTHFSVHLLPKYFK 60

Query: 61  HSNFSSFMRQLNIYGFRKIDTDCWEFATDGFVKGQKHLLKNIYRRKNIQATDQRKASQPQ 120
           HSNFSSFMRQLNIYGFRKI+TDCWEFATDGFVKGQKHLLKNIYRRKNIQ TDQRKASQPQ
Sbjct: 61  HSNFSSFMRQLNIYGFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQRKASQPQ 120

Query: 121 DNSEGQGELHDYTGLWKEVENLKIDKNAVMQELVKLRQQQETSENKLLLLRDRLQGMEKN 180
           DNSEGQGELHDYTGLWKEVENLKIDKNAVMQELVKLRQQQETSENK+LLLRDRLQGMEKN
Sbjct: 121 DNSEGQGELHDYTGLWKEVENLKIDKNAVMQELVKLRQQQETSENKMLLLRDRLQGMEKN 180

Query: 181 QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGMIVRYQRPI 240
           QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNG+IVRYQRPI
Sbjct: 181 QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGLIVRYQRPI 240

Query: 241 DERPATLLPPVSGLGKQLESDPFPDGMKEFFLNSDFMKMLMDEKLDNHSQFVLPDVQDVA 300
           DE PA LLPPVSGLGKQ ESDPFPDGMK+FFLNSDFMK+LMDEKLDNHSQFVLPDVQDVA
Sbjct: 241 DELPAPLLPPVSGLGKQQESDPFPDGMKDFFLNSDFMKVLMDEKLDNHSQFVLPDVQDVA 300

Query: 301 WEQ--LASPFSWNLDNVRKVDDEREPTDHDDHEDHELDMETIDTRTHEENSQDFELLIRQ 360
           WEQ  LAS  S N DNVRKVDDEREP    DHEDHELDMETIDTRTHEEN QDFELLI+Q
Sbjct: 301 WEQLLLASTVSCNSDNVRKVDDEREPM---DHEDHELDMETIDTRTHEENLQDFELLIKQ 360

Query: 361 MEKGENFGMQPGLDETYIEKSNTVNRLAQQMELLASDQEILYETPEKMQAY 410
           MEKGENFG+QP LDE+YIEKSNTVN     MEL+ASDQEILYETP KMQ +
Sbjct: 361 MEKGENFGLQPRLDESYIEKSNTVN----LMELMASDQEILYETPAKMQGH 404

BLAST of Tan0000033 vs. ExPASy TrEMBL
Match: A0A6J1K754 (heat stress transcription factor A-8-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491273 PE=3 SV=1)

HSP 1 Score: 717.2 bits (1850), Expect = 3.8e-203
Identity = 368/411 (89.54%), Postives = 382/411 (92.94%), Query Frame = 0

Query: 1   MVKSTAERQGSSVAPFLKKLYDMVDDEATNSIISWSPTDDSFTISDMTQFSIHLLPKYFK 60
           MVKS  ERQGSSVAPFLKKLY+MVDDEATNSIISWSP DDSFTI DMT FS+HLLPKYFK
Sbjct: 1   MVKSAGERQGSSVAPFLKKLYEMVDDEATNSIISWSPRDDSFTILDMTHFSVHLLPKYFK 60

Query: 61  HSNFSSFMRQLNIYGFRKIDTDCWEFATDGFVKGQKHLLKNIYRRKNIQATDQRKASQPQ 120
           HSNFSSFMRQLNIYGFRKI+TDCWEFATDGFVKGQKHLLKNIYRRKNIQ TDQR+ASQPQ
Sbjct: 61  HSNFSSFMRQLNIYGFRKIETDCWEFATDGFVKGQKHLLKNIYRRKNIQGTDQRRASQPQ 120

Query: 121 DNSEGQGELHDYTGLWKEVENLKIDKNAVMQELVKLRQQQETSENKLLLLRDRLQGMEKN 180
           DNSEGQGELHDYTGLWKEVENLKIDKN VMQELVKLRQQQETSE+K+LLLRDRLQGMEKN
Sbjct: 121 DNSEGQGELHDYTGLWKEVENLKIDKNGVMQELVKLRQQQETSESKMLLLRDRLQGMEKN 180

Query: 181 QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGMIVRYQRPI 240
           QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNG+IVRYQRPI
Sbjct: 181 QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGLIVRYQRPI 240

Query: 241 DERPATLLPPVSGLGKQLESDPFPDGMKEFFLNSDFMKMLMDEKLDNHSQFVLPDVQDVA 300
           DE PA LLPPVSGLGKQ ESDPFPDGMK+FFLNSDFMK+LMDEKLDNHSQFVLPDVQDVA
Sbjct: 241 DELPAPLLPPVSGLGKQQESDPFPDGMKDFFLNSDFMKVLMDEKLDNHSQFVLPDVQDVA 300

Query: 301 WEQ--LASPFSWNLDNVRKVDDEREPTDHDDHEDHELDMETIDTRTHEENSQDFELLIRQ 360
           WEQ  LAS  S N DNVRKVDDEREP    DHEDHELDMETIDTRTHEEN QDFELLI+Q
Sbjct: 301 WEQLLLASTVSCNSDNVRKVDDEREPM---DHEDHELDMETIDTRTHEENLQDFELLIKQ 360

Query: 361 MEKGENFGMQPGLDETYIEKSNTVNRLAQQMELLASDQEILYETPEKMQAY 410
           MEKGENFG+QP LDE+YIEKSNTVN     MEL+ASDQEILYETP KMQ +
Sbjct: 361 MEKGENFGLQPRLDESYIEKSNTVN----LMELMASDQEILYETPAKMQGH 404

BLAST of Tan0000033 vs. ExPASy TrEMBL
Match: A0A1S3CMJ8 (heat stress transcription factor A-8 OS=Cucumis melo OX=3656 GN=LOC103502515 PE=3 SV=1)

HSP 1 Score: 656.4 bits (1692), Expect = 8.0e-185
Identity = 342/411 (83.21%), Postives = 372/411 (90.51%), Query Frame = 0

Query: 1   MVKSTAERQGSSVAPFLKKLYDMVDDEATNSIISWSPTDDSFTISDMTQFSIHLLPKYFK 60
           MVKS A+RQGSSVAPFLKKLYDMVDD++TNSIISW+ ++DSFTI D+TQFS+HLLPKYFK
Sbjct: 1   MVKSAADRQGSSVAPFLKKLYDMVDDDSTNSIISWTSSNDSFTILDITQFSLHLLPKYFK 60

Query: 61  HSNFSSFMRQLNIYGFRKIDTDCWEFATDGFVKGQKHLLKNIYRRKNIQATDQRKASQPQ 120
           HSNFSSFMRQLNIYGFRKIDTDCWEFATDGFVKGQKHLLK+IYRRKNIQ TDQRKA QP+
Sbjct: 61  HSNFSSFMRQLNIYGFRKIDTDCWEFATDGFVKGQKHLLKSIYRRKNIQGTDQRKALQPR 120

Query: 121 DNSEGQGELHDYTGLWKEVENLKIDKNAVMQELVKLRQQQETSENKLLLLRDRLQGMEKN 180
           DNSEGQ EL DY+GLWKEVENLKIDKNAVMQELVKL+Q QETSENKLLLLR+RLQGMEKN
Sbjct: 121 DNSEGQVELPDYSGLWKEVENLKIDKNAVMQELVKLKQHQETSENKLLLLRERLQGMEKN 180

Query: 181 QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGMIVRYQRPI 240
           QQQMLSFLVMAVQSPGFLVQFLQPKEK+WRMAD  NMLEQI DDNQVPSNGMIVRYQRP+
Sbjct: 181 QQQMLSFLVMAVQSPGFLVQFLQPKEKSWRMADPSNMLEQISDDNQVPSNGMIVRYQRPL 240

Query: 241 DERPATLLPPVSGLGKQLESDPFPDGMKEFFLNSDFMKMLMDEK--LDNHSQFVLPDVQD 300
           DE   TLLPPV+G GKQ ES+PFPDGMK+FFLNSDFMK+LMDEK  LDNHSQFVLPDVQD
Sbjct: 241 DELSTTLLPPVTGPGKQQESEPFPDGMKDFFLNSDFMKVLMDEKVCLDNHSQFVLPDVQD 300

Query: 301 VAWEQ--LASPFSWNLDNVRKVDDEREPTDHDDHEDHELDMETIDTRTHEENSQDFELLI 360
           VAWEQ  LA+PFS N +N RKVD ER  T   D ED ELDMETIDT+THEENSQDFELLI
Sbjct: 301 VAWEQLLLANPFSGNSENGRKVDHERRYT---DSEDDELDMETIDTQTHEENSQDFELLI 360

Query: 361 RQMEKGENFGMQPGLDETYIEKSNTVNRLAQQMELLASDQEILYETPEKMQ 408
           RQMEK E+F +Q  LDE+YIE SN V+ L QQM+LLASD+EILYETP+KM+
Sbjct: 361 RQMEKCEDFEIQTRLDESYIENSNDVHLLTQQMDLLASDREILYETPDKMR 408

BLAST of Tan0000033 vs. ExPASy TrEMBL
Match: A0A0A0KMJ2 (HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G025910 PE=3 SV=1)

HSP 1 Score: 644.8 bits (1662), Expect = 2.4e-181
Identity = 334/399 (83.71%), Postives = 360/399 (90.23%), Query Frame = 0

Query: 1   MVKSTAERQGSSVAPFLKKLYDMVDDEATNSIISWSPTDDSFTISDMTQFSIHLLPKYFK 60
           MVKS AERQGSSVAPFLKKLYDMVDD++TNS+ISW+ ++DSFTI D+T FS+HLLPKYFK
Sbjct: 1   MVKSAAERQGSSVAPFLKKLYDMVDDDSTNSVISWTSSNDSFTILDITHFSLHLLPKYFK 60

Query: 61  HSNFSSFMRQLNIYGFRKIDTDCWEFATDGFVKGQKHLLKNIYRRKNIQATDQRKASQPQ 120
           HSNFSSFMRQLNIYGFRKIDTDCWEFATDGF+KGQKHLLKNIYRRKNI  TDQRKASQPQ
Sbjct: 61  HSNFSSFMRQLNIYGFRKIDTDCWEFATDGFIKGQKHLLKNIYRRKNIHGTDQRKASQPQ 120

Query: 121 DNSEGQGELHDYTGLWKEVENLKIDKNAVMQELVKLRQQQETSENKLLLLRDRLQGMEKN 180
           DNSE Q EL DY+GLWKEVENLKIDKNAVMQELVKL+Q QETSENKLLLLR+RLQGMEKN
Sbjct: 121 DNSEAQVELPDYSGLWKEVENLKIDKNAVMQELVKLKQHQETSENKLLLLRERLQGMEKN 180

Query: 181 QQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGMIVRYQRPI 240
           QQQMLSFLVMAVQSPGFLVQFLQPKEK+WRMAD GNMLEQI DDNQVPSNGMIVRYQRP+
Sbjct: 181 QQQMLSFLVMAVQSPGFLVQFLQPKEKSWRMADPGNMLEQISDDNQVPSNGMIVRYQRPL 240

Query: 241 DERPATLLPPVSGLGKQLESDPFPDGMKEFFLNSDFMKMLMDEK--LDNHSQFVLPDVQD 300
           DE   TLLPPV+G GKQ ES+PFPDGMK+FFLNSDFMK+LMDEK  LDNHSQFVLPDVQD
Sbjct: 241 DELSTTLLPPVTGPGKQQESEPFPDGMKDFFLNSDFMKVLMDEKVCLDNHSQFVLPDVQD 300

Query: 301 VAWEQ--LASPFSWNLDNVRKVDDEREPTDHDDHEDHELDMETIDTRTHEENSQDFELLI 360
           VAWEQ  LA+PFS N DN RKVD ER  T   D ED ELDMETIDT+THEENS+DFELLI
Sbjct: 301 VAWEQLLLANPFSGNSDNGRKVDHERRYT---DSEDDELDMETIDTQTHEENSEDFELLI 360

Query: 361 RQMEKGENFGMQPGLDETYIEKSNTVNRLAQQMELLASD 396
           RQMEK E+FG+QP LDE+YIE SN V+ L QQM+ LASD
Sbjct: 361 RQMEKCEDFGIQPRLDESYIENSNDVHLLTQQMDYLASD 396

BLAST of Tan0000033 vs. ExPASy TrEMBL
Match: A0A0S2IAH3 (Heat stress transcription factor A-8 OS=Momordica charantia OX=3673 GN=LOC111015865 PE=2 SV=1)

HSP 1 Score: 634.4 bits (1635), Expect = 3.3e-178
Identity = 336/402 (83.58%), Postives = 351/402 (87.31%), Query Frame = 0

Query: 1   MVKSTAERQGSSVAPFLKKLYDMVDDEATNSIISWSPTDDSFTISDMTQFSIHLLPKYFK 60
           MVKS AER+G S APFL KLY MVDDEATNSIISWSPTDDSFTI DMTQFS+ LLPKYFK
Sbjct: 1   MVKSAAERKGPSAAPFLNKLYQMVDDEATNSIISWSPTDDSFTILDMTQFSVLLLPKYFK 60

Query: 61  HSNFSSFMRQLNIYGFRKIDTDCWEFATDGFVKGQKHLLKNIYRRKNIQATDQRKASQP- 120
           HSNFSSFMRQLNIYGFRKIDTDCWEFATDGFVKGQKHLLKNI RRKNIQ TDQRKASQP 
Sbjct: 61  HSNFSSFMRQLNIYGFRKIDTDCWEFATDGFVKGQKHLLKNICRRKNIQGTDQRKASQPA 120

Query: 121 QDNSEGQGELHDYTGLWKEVENLKIDKNAVMQELVKLRQQQETSENKLLLLRDRLQGMEK 180
           Q+NSEGQGELHDYTGLWKEVENLKIDKNA+MQELVKLRQ QETSENKLLLLRDRLQGMEK
Sbjct: 121 QENSEGQGELHDYTGLWKEVENLKIDKNALMQELVKLRQHQETSENKLLLLRDRLQGMEK 180

Query: 181 NQQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQVPSNGMIVRYQRP 240
           NQQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMA+AG +LEQIPDDNQVPSNGMIVRYQRP
Sbjct: 181 NQQQMLSFLVMAVQSPGFLVQFLQPKEKNWRMAEAGTILEQIPDDNQVPSNGMIVRYQRP 240

Query: 241 IDERPATLLPPVSGLGKQLESDPFPDGMKEFFLNSDFMKMLMDEK--LDNHSQFVLPDVQ 300
           +DE PATLLPPVSG GK  ES+PFP+GMK+FFLNSDFMK+LMDEK  LDNHS FVLPDVQ
Sbjct: 241 LDELPATLLPPVSGSGKHQESEPFPEGMKDFFLNSDFMKVLMDEKLCLDNHSPFVLPDVQ 300

Query: 301 DVAWEQ--LASPFSWNLDNVRKVDDEREPTDHDDHEDHELDMETIDTRTHEENSQDFELL 360
           D+AWEQ  LAS FS N +N RKVDD             ELDMET DT T EE SQDFELL
Sbjct: 301 DIAWEQLLLASHFSGNSENGRKVDD-------------ELDMETTDTGTDEEKSQDFELL 360

Query: 361 IRQMEKGENFGMQPGLDETYIEKSNTVNRLAQQMELLASDQE 398
           +RQMEK EN GM    DE+ IEKSNTVN L QQMELLASDQE
Sbjct: 361 VRQMEKCENSGMPLRFDESCIEKSNTVNLLTQQMELLASDQE 389

BLAST of Tan0000033 vs. TAIR 10
Match: AT1G67970.1 (heat shock transcription factor A8 )

HSP 1 Score: 290.4 bits (742), Expect = 2.2e-78
Identity = 186/405 (45.93%), Postives = 250/405 (61.73%), Query Frame = 0

Query: 1   MVKST----AERQGSSVAPFLKKLYDMVDDEATNSIISWSPT-DDSFTISDMTQFSIHLL 60
           MVKST         SSVAPFL+K YDMVDD  T+SIISWSP+ D+SF I D T FS+ LL
Sbjct: 1   MVKSTDGGGGSSSSSSVAPFLRKCYDMVDDSTTDSIISWSPSADNSFVILDTTVFSVQLL 60

Query: 61  PKYFKHSNFSSFMRQLNIYGFRKIDTDCWEFATDGFVKGQKHLLKNIYRRKNIQATDQRK 120
           PKYFKHSNFSSF+RQLNIYGFRK+D D WEFA DGFV+GQK LLKN+ RRKN+Q+++Q K
Sbjct: 61  PKYFKHSNFSSFIRQLNIYGFRKVDADRWEFANDGFVRGQKDLLKNVIRRKNVQSSEQSK 120

Query: 121 ASQPQDNSEGQGELHDYTGLWKEVENLKIDKNAVMQELVKLRQQQETSENKLLLLRDRLQ 180
                  S       + +GLWKEV+ LK DK  + QEL+K+RQ QE ++ K+L L DR+Q
Sbjct: 121 -----HESTSTTYAQEKSGLWKEVDILKGDKQVLAQELIKVRQYQEVTDTKMLHLEDRVQ 180

Query: 181 GMEKNQQQMLSFLVMAVQSPGFLVQFLQPKEKN-WRMADAG-NMLEQIPDDNQVPSNGM- 240
           GME++QQ+MLSFLVM +++P  LVQ LQPKEKN WR A  G  ++E++ D+ +  S G+ 
Sbjct: 181 GMEESQQEMLSFLVMVMKNPSLLVQLLQPKEKNTWRKAGEGAKIVEEVTDEGESNSYGLP 240

Query: 241 IVRYQRPIDERPATLLPPVSGLGKQLESDPFPDGMKEFFLNSDFMKMLMDEKLDNHSQFV 300
           +V YQ P D          +G  K   +D     + +F  N+D +K  +DE   NH   +
Sbjct: 241 LVTYQPPSDN---------NGTAKSNSND-----VNDFLRNADMLKFCLDE---NHVPLI 300

Query: 301 LPDV-QDVAWEQLASPFSWNLDNVRKVDDEREPTDHDDHEDHELDMETIDTRTHEENSQD 360
           +PD+  D AWE+L       L   RK   ++E       +D  L+ E  D     + S  
Sbjct: 301 IPDLYDDGAWEKLLL-----LSPSRKKTKKQENIVKKGKDDLTLEEEEEDGTMELDKSYM 360

Query: 361 FELLIRQMEKGENFGMQPGLDETYIEKSNTVNRLAQQMELLASDQ 397
            +L+  +MEK ++F       +   E+S  +  L +QMELLAS++
Sbjct: 361 LKLISEEMEKPDDF----EFGQLTPERSRNLEILTEQMELLASNE 374

BLAST of Tan0000033 vs. TAIR 10
Match: AT4G17750.1 (heat shock factor 1 )

HSP 1 Score: 219.2 bits (557), Expect = 6.3e-57
Identity = 123/244 (50.41%), Postives = 163/244 (66.80%), Query Frame = 0

Query: 15  PFLKKLYDMVDDEATNSIISWSPTDDSFTISDMTQFSIHLLPKYFKHSNFSSFMRQLNIY 74
           PFL K YDMV+D AT++I+SWSPT++SF + D  +FS  LLPKYFKH+NFSSF+RQLN Y
Sbjct: 52  PFLSKTYDMVEDPATDAIVSWSPTNNSFIVWDPPEFSRDLLPKYFKHNNFSSFVRQLNTY 111

Query: 75  GFRKIDTDCWEFATDGFVKGQKHLLKNIYRRKNIQATDQRKAS-QPQDNSEGQGELHDYT 134
           GFRK+D D WEFA +GF++GQKHLLK I RRK++Q      ++ Q Q  S+GQG +   +
Sbjct: 112 GFRKVDPDRWEFANEGFLRGQKHLLKKISRRKSVQGHGSSSSNPQSQQLSQGQGSMAALS 171

Query: 135 --------GLWKEVENLKIDKNAVMQELVKLRQQQETSENKLLLLRDRLQGMEKNQQQML 194
                   GL +EVE LK DKN +MQELVKLRQQQ+T++NKL +L   LQ ME+ QQQ++
Sbjct: 172 SCVEVGKFGLEEEVEQLKRDKNVLMQELVKLRQQQQTTDNKLQVLVKHLQVMEQRQQQIM 231

Query: 195 SFLVMAVQSPGFLVQFLQPKEKNWRMADAGNMLEQIPDDNQV------------PSNGMI 238
           SFL  AVQ+P FL QF+Q +  +       N   ++ +D+               S+G I
Sbjct: 232 SFLAKAVQNPTFLSQFIQKQTDSNMHVTEANKKRRLREDSTAATESNSHSHSLEASDGQI 291

BLAST of Tan0000033 vs. TAIR 10
Match: AT5G16820.1 (heat shock factor 3 )

HSP 1 Score: 210.7 bits (535), Expect = 2.2e-54
Identity = 121/247 (48.99%), Postives = 160/247 (64.78%), Query Frame = 0

Query: 11  SSVAPFLKKLYDMVDDEATNSIISWSPTDDSFTISDMTQFSIHLLPKYFKHSNFSSFMRQ 70
           +SV PFL K YDMVDD  TN ++SWS  ++SF +    +FS  LLPKYFKH+NFSSF+RQ
Sbjct: 23  NSVPPFLSKTYDMVDDPLTNEVVSWSSGNNSFVVWSAPEFSKVLLPKYFKHNNFSSFVRQ 82

Query: 71  LNIYGFRKIDTDCWEFATDGFVKGQKHLLKNIYRRKNIQATDQRKASQPQDNSEGQGELH 130
           LN YGFRK+D D WEFA +GF++G+K LLK+I RRK       ++ +Q Q +S G     
Sbjct: 83  LNTYGFRKVDPDRWEFANEGFLRGRKQLLKSIVRRKPSHVQQNQQQTQVQSSSVGACVEV 142

Query: 131 DYTGLWKEVENLKIDKNAVMQELVKLRQQQETSENKLLLLRDRLQGMEKNQQQMLSFLVM 190
              G+ +EVE LK DKN +MQELV+LRQQQ+ +EN+L  +  ++Q ME+ QQQM+SFL  
Sbjct: 143 GKFGIEEEVERLKRDKNVLMQELVRLRQQQQATENQLQNVGQKVQVMEQRQQQMMSFLAK 202

Query: 191 AVQSPGFLVQFLQPKEKNW-RMADAGNMLEQIPDDNQV-----PSNGM---IVRYQRPID 249
           AVQSPGFL Q +Q    +  R     N   ++P D Q       +NG+   IVRYQ  I+
Sbjct: 203 AVQSPGFLNQLVQQNNNDGNRQIPGSNKKRRLPVDEQENRGDNVANGLNRQIVRYQPSIN 262

BLAST of Tan0000033 vs. TAIR 10
Match: AT5G16820.2 (heat shock factor 3 )

HSP 1 Score: 210.7 bits (535), Expect = 2.2e-54
Identity = 121/247 (48.99%), Postives = 160/247 (64.78%), Query Frame = 0

Query: 11  SSVAPFLKKLYDMVDDEATNSIISWSPTDDSFTISDMTQFSIHLLPKYFKHSNFSSFMRQ 70
           +SV PFL K YDMVDD  TN ++SWS  ++SF +    +FS  LLPKYFKH+NFSSF+RQ
Sbjct: 23  NSVPPFLSKTYDMVDDPLTNEVVSWSSGNNSFVVWSAPEFSKVLLPKYFKHNNFSSFVRQ 82

Query: 71  LNIYGFRKIDTDCWEFATDGFVKGQKHLLKNIYRRKNIQATDQRKASQPQDNSEGQGELH 130
           LN YGFRK+D D WEFA +GF++G+K LLK+I RRK       ++ +Q Q +S G     
Sbjct: 83  LNTYGFRKVDPDRWEFANEGFLRGRKQLLKSIVRRKPSHVQQNQQQTQVQSSSVGACVEV 142

Query: 131 DYTGLWKEVENLKIDKNAVMQELVKLRQQQETSENKLLLLRDRLQGMEKNQQQMLSFLVM 190
              G+ +EVE LK DKN +MQELV+LRQQQ+ +EN+L  +  ++Q ME+ QQQM+SFL  
Sbjct: 143 GKFGIEEEVERLKRDKNVLMQELVRLRQQQQATENQLQNVGQKVQVMEQRQQQMMSFLAK 202

Query: 191 AVQSPGFLVQFLQPKEKNW-RMADAGNMLEQIPDDNQV-----PSNGM---IVRYQRPID 249
           AVQSPGFL Q +Q    +  R     N   ++P D Q       +NG+   IVRYQ  I+
Sbjct: 203 AVQSPGFLNQLVQQNNNDGNRQIPGSNKKRRLPVDEQENRGDNVANGLNRQIVRYQPSIN 262

BLAST of Tan0000033 vs. TAIR 10
Match: AT1G32330.1 (heat shock transcription factor A1D )

HSP 1 Score: 209.5 bits (532), Expect = 5.0e-54
Identity = 135/328 (41.16%), Postives = 190/328 (57.93%), Query Frame = 0

Query: 15  PFLKKLYDMVDDEATNSIISWSPTDDSFTISDMTQFSIHLLPKYFKHSNFSSFMRQLNIY 74
           PFL K YDMVDD  T+SI+SWS  ++SF +    +F+  LLPK FKH+NFSSF+RQLN Y
Sbjct: 37  PFLSKTYDMVDDHNTDSIVSWSANNNSFIVWKPPEFARDLLPKNFKHNNFSSFVRQLNTY 96

Query: 75  GFRKIDTDCWEFATDGFVKGQKHLLKNIYRRKNIQATDQRKASQPQDNSEGQG------- 134
           GFRK+D D WEFA +GF++GQKHLL++I RRK   A  Q +  Q   +S GQ        
Sbjct: 97  GFRKVDPDRWEFANEGFLRGQKHLLQSITRRK--PAHGQGQGHQRSQHSNGQNSSVSACV 156

Query: 135 ELHDYTGLWKEVENLKIDKNAVMQELVKLRQQQETSENKLLLLRDRLQGMEKNQQQMLSF 194
           E+  + GL +EVE LK DKN +MQELV+LRQQQ++++N+L  +  RLQGME  QQQ++SF
Sbjct: 157 EVGKF-GLEEEVERLKRDKNVLMQELVRLRQQQQSTDNQLQTMVQRLQGMENRQQQLMSF 216

Query: 195 LVMAVQSPGFLVQFLQPK----EKNWRMADAGNMLE-----QIPDDNQVPSNGMIVRYQR 254
           L  AVQSP FL QFLQ +    E N R++D            + +++    +G IV+YQ 
Sbjct: 217 LAKAVQSPHFLSQFLQQQNQQNESNRRISDTSKKRRFKRDGIVRNNDSATPDGQIVKYQP 276

Query: 255 PIDERPATLLPPVSGLGKQLESDPFPDGMKEFFL---NSDFMKMLMDEKLDNHSQFVLPD 314
           P+ E+   +   +      ++ +P+  G   F L    S      M+   +  S   L +
Sbjct: 277 PMHEQAKAMFKQL------MKMEPYKTGDDGFLLGNGTSTTEGTEMETSSNQVSGITLKE 336

Query: 315 VQDVAWEQLASPFSWNLDNVRKVDDERE 324
           +   +  Q +SP     +NV    +  E
Sbjct: 337 MPTASEIQSSSPIETTPENVSAASEATE 355

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9S7U53.1e-7745.93Heat stress transcription factor A-8 OS=Arabidopsis thaliana OX=3702 GN=HSFA8 PE... [more]
Q401525.5e-5847.10Heat shock factor protein HSF8 OS=Solanum lycopersicum OX=4081 GN=HSF8 PE=3 SV=1[more]
P411531.2e-5746.74Heat shock factor protein HSF8 OS=Solanum peruvianum OX=4082 GN=HSF8 PE=2 SV=1[more]
P411518.8e-5650.41Heat stress transcription factor A-1a OS=Arabidopsis thaliana OX=3702 GN=HSFA1A ... [more]
Q84T612.0e-5546.30Heat stress transcription factor A-1 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Match NameE-valueIdentityDescription
KAG6599929.16.4e-20590.27Heat stress transcription factor A-8, partial [Cucurbita argyrosperma subsp. sor... [more]
XP_022942461.13.2e-20490.27heat stress transcription factor A-8-like isoform X1 [Cucurbita moschata][more]
XP_023536181.12.7e-20390.02heat stress transcription factor A-8-like isoform X1 [Cucurbita pepo subsp. pepo... [more]
XP_022995879.17.9e-20389.54heat stress transcription factor A-8-like isoform X1 [Cucurbita maxima][more]
XP_038892281.11.7e-18985.16heat stress transcription factor A-8 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1FNX41.5e-20490.27heat stress transcription factor A-8-like isoform X1 OS=Cucurbita moschata OX=36... [more]
A0A6J1K7543.8e-20389.54heat stress transcription factor A-8-like isoform X1 OS=Cucurbita maxima OX=3661... [more]
A0A1S3CMJ88.0e-18583.21heat stress transcription factor A-8 OS=Cucumis melo OX=3656 GN=LOC103502515 PE=... [more]
A0A0A0KMJ22.4e-18183.71HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G025910 ... [more]
A0A0S2IAH33.3e-17883.58Heat stress transcription factor A-8 OS=Momordica charantia OX=3673 GN=LOC111015... [more]
Match NameE-valueIdentityDescription
AT1G67970.12.2e-7845.93heat shock transcription factor A8 [more]
AT4G17750.16.3e-5750.41heat shock factor 1 [more]
AT5G16820.12.2e-5448.99heat shock factor 3 [more]
AT5G16820.22.2e-5448.99heat shock factor 3 [more]
AT1G32330.15.0e-5441.16heat shock transcription factor A1D [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 139..173
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 109..128
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 112..127
NoneNo IPR availablePANTHERPTHR10015:SF325HEAT STRESS TRANSCRIPTION FACTOR A-8coord: 1..334
IPR000232Heat shock factor (HSF)-type, DNA-bindingPRINTSPR00056HSFDOMAINcoord: 54..66
score: 78.02
coord: 16..39
score: 46.67
coord: 67..79
score: 76.26
IPR000232Heat shock factor (HSF)-type, DNA-bindingSMARTSM00415hsfneu3coord: 12..105
e-value: 1.7E-53
score: 193.7
IPR000232Heat shock factor (HSF)-type, DNA-bindingPFAMPF00447HSF_DNA-bindcoord: 16..105
e-value: 2.2E-30
score: 105.1
IPR000232Heat shock factor (HSF)-type, DNA-bindingPROSITEPS00434HSF_DOMAINcoord: 55..79
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 8..106
e-value: 4.2E-34
score: 119.1
IPR027725Heat shock transcription factor familyPANTHERPTHR10015HEAT SHOCK TRANSCRIPTION FACTORcoord: 1..334
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 11..105

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0000033.1Tan0000033.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034605 cellular response to heat
biological_process GO:0006357 regulation of transcription by RNA polymerase II
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
molecular_function GO:0043565 sequence-specific DNA binding