Spg039061 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg039061
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptiontRNA threonylcarbamoyladenosine dehydratase
Locationscaffold12: 1630089 .. 1638176 (+)
RNA-Seq ExpressionSpg039061
SyntenySpg039061
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTCTTAGCTCTTATGTTATCAATACCAAGCCCTCCAAACGATTTAGGAGTTGTCACAGCCTCCAAATTCACCAAATGAGATCCTTTAATATCGTCAGCCCCTTTCCAAAGGAATCTTCTAAAATACTTCTCAATGCATTTGCTCACCCCCACTGGTATCTTGAATGTAGAAAGAAAATAGGTTGGGATGCTAGATAGAACAACCTTTATGAGGGTTGTTCTTCCACCTTTGGAGATGGGGAAACCTTTCCAAAGAGCTAACTTGCTGCGAATGTTGTCTATTATCGGTTCCCAGAACGCTTCCTTTAATGAACCCTTTACTTTTGCAAACACCAATATAGCGGCTCAAGGAATCAACAATTAAAGTAAAAAGGAAGGGAGACAGAGGGTCACCTTGTCTTATGCCTCTGGAAGCTGAAATTTTCCCTCTGGGCCTCCCATTTATCAAAATAGAAAAGTTGGAATTCATGCAGCAACCTCTCATCCATCTTCTCCATCTATCACTGAAGCCTTTGGCCTGAAGAGTAAAGTCTAGAAAACCCTAATCCACTTTGTCATAAGCCTTTTCAAAGTCAACTTTAATCAAAAAGCCTCTTTTCTGCGAAACACTATAGTCATCTACAGCTTTTGCCGCCATTAAAATAGCATCCATAATCTGTCTACCTTCCACAAACGCCATTTGAGAACTATCTATCGTAGAGGGGAGAACTTTTATGATTCTTTCAGCAAGCACTTTGGAAATAGATTTGTACAACACCGTGACCAGGCTAATGGGGCGGTAATTGTTAACTTTGCTAGCCACTTTTTTCTTTGGGATTAAGCACAGGTAAGTCTCATTGGTCTTCTTATGAATGATCTCGTTCTCAAAAAACTTCTGGAACACCTTAAATAAGTCATCTTTAATAGTGTTCCAAGAGTTTTTAAAAAATTCACCAGTAAAACCATCCGGCCCCGGCGACTTTTGAGCTCCCAACATGGATTTTGCTTTGAAGATTTCCTCCATTTCAAAAGGCCTTTCCAGCCATCGAGCTTGGTTTTCCGATATTTGACCCCAATCAACCTCTTTCAACGTGCAGCGCTGTTCCCCATCACACCTGTATAGCTGCTGGTAAAAACTGATAATCTCTTCCTCTATATCTTCTTTCTTGGTGAGGAGCTCCCCTTCTTTGTTAATAAGGGAGGAGATAAAACTTTTGTTTTTTCTGTACGAAGACTATCTATGGAAGAATTTCGAATTAACATCACCTTCCTTCAGCCATTTAATTTTCATTTTTTGCAGCCAAGACCTCTGCTCATTTAGAATAATGTCAGCTAGGGAAGCCTGCAGATTCTTTCTTTCAACAAAGAGGTTCTCATCGAGTGGCCCTTCTTCTTCAAGGCTATCTATACGGCTTATACTTTGACAAATCTTTTCCTTTTCCTCCCTTATTACGCCAAAAGTCTCCTTGTTCCAAACTTTAAGGTTGGCTTTGATGCCTTTTAGCTTTTCCATGAAACAGTAACTAGCCCAACCCTCAGAGTTTGTTTGCTTCCACCAAACATCAACTAAATTTCTAAAGTCAGGATGAGATAACCAAGCATTCTCAAATCTAAACGGCGTTGGACCCCACGAAAAATTCCCAGCTTGAAGCAAAATAGGGAAGTGGTCTGAGGTGACCCGTTGCAATCGAAGAACACTAACATTGGAGAATAGGTCGACCCAATCCGTGGAAACCAAAAATCTATCAATTTTAGTGAAAACCGGAGCCTCCCTTTTATCAGACCACGTATACTTGGCATTGGGCATAACAACCTCAACCAAACTGCAGTTGTCAATCCATAGTTTCCACCCACATCTTCCCAAAATCTAATGGAGGCTCCATTGACAACCTTGTAAATTGACCTCTCTCTTCGATAATTGTTTGACATTTTGAGATAGAGAACCGAGGACCCCTGTTGCTGCTATGCTAACAAGTGAGGGTAGACCACCTTGAAAATATCCTATATTTACATGCAATAATTTTGGCTCTCCAGTTGTACACAATTAATTTGAGATTAATAAAATAACTTGGGTTTTCCTTACCTCATCAAGGAATGTTTTATGTTTAGCATTAATCTGCTTTGCTTTTAACATGTTAGTCATATGTCTATTGTGATTAGGAACATTCAGTTCTTTGGCCTTAGCTCACAGAAGAATGTGACTGCATCTTATGTTGTGGTAATTGGTCTTGGTGGTGTTGGGAGTCATGCTGCTATGATGCTTCTACGATCAGGGGTTGGCAGGCTTCTCCTTGTTGACTTTGACCAGGTCTTTTACATTATCTTTCAGGAAAATGATGACTCTTGTATACAATCTGTTTGATTTCTACCACCTTGTGGAGTTGCTGACTTCCACTGCTTGCTAATCAGAAAAATATCTCTTTGCCCTCTTATGAATGTCATTGCAGATGACTTCAATCATCTTCATGTCACGTTTTAGCGTTCATTAACTTTTTATATCCAATGAAATACTTTTATATAGTTCATGTATTTTAGAATGTTGTGAGTTTTTCCCCCGTCTTCCAATTTCTTAACACATCTGTTTTCCAATTTTGAGTTTTTTATTTGCATAGATTTTCATTATGACTATATACATACATCCGTACATATATAAAATACACCAGCTTTCGTCATAGAAATTGATTGTTACATTATATCTACATCTAATAAGAGCATAGCTCAACTAGCATAAAGTTTATACTCTCGACTAAGAGATAAGAGGTTCAAATCCCTCACCCCACATGTTGTAAAAAAAAAAGTATGAAAAAGAATCAATGCATTTAAATTTTCTTTTGTAGCATGTATACTCATTTGTTAATTACTTGAAAAGGTGTCACTTTCTTCTCTAAATCGACATGCCGTTGCAACAAGAGCAGATGTAGGTGTCCCAAAAGCTCAGTGCCTGAAGGAGCACTTTCTGTCCATCTTTCCAGAGTGTCAAGTAGAAGCAAAAGTGCTATTATATGATGCAACATCTGAAGAAGAAATTCTTTGTGGAAAACCTGATTTTGTTTTGGATTGCATTGACAATATTGATACAAAGGTGACTATTTATCTGCAAGACTTTAACCTATGTTAAATCTTCTTATCTCTCTCTCTCTTTAATAATTATTTTTAAATTTTCTATTAGCAGTGACTTTAATATTGATGCTAAATTGACATATAGAAAGGATAAATAAGGACGATGGAATTTAGAAAATTAATGTACTTTCAGAAAGTATAAATGAATCATTAATTCACCATTTATTGAGGTTTAATTACCAAAACCGTCTTTTTGAAACACTATACATTTGGGGATGGGGTATTGATAGGGCCTCCTGTTATGTTTACATATGCGAGGAAGAATAGGAGAAGGGAGAAGGGCCCTAAAGATGGGAACCAATTTTGCATAGCTTTCTCATTCCAGTGGGCCTAAGTTAAATAATTAAAAGCAAGTAGTTAGAGAAGAGAATAATATAAAGAGGGAATGTATGTGAAAGGGTGAGTAAGAATAGTTATAGTGCATGCAACATCTTTGGAGTCGGAGAAATCAAGATCATCAAATTTAACCTAAGTTTATAATGAAATATTGAAAGGACTGTTTAATGAGGTGGATGGAAATGCATGTGTAAATTACGTAATGTTGTTTTGGGCGATAAAATTTCCATGTAACAAGCTTTTTAGCCAAAGGAATAGTTTGTTTATTTGTTTGGTTGTTTGTTTGTTTGTTTTTTTTTTTCATTTTAAGCAATTGATGTTGTCGAATTTTACTTCTATTTATGGATGTTCATATTTGAATGTGCTTTTCTAATAGGAGTTTGTTTTTAAATGCTTTGTAAATCATATGTGATAGGTATCACTTCTTGCTGCATGTGTACGTAGGGGTTTGAAAGTTTTATCAGCAACAGGAGCCGGAGCTCGAGCTGACCCAACAAGAATTCGTGTGGCTGATTTAAGAGAGTCAACAAATGACCCATTATCTCGAGCTGTAATGTTTCTTCATGTGACAAAAATTTTCTTTTACGCCAAAACTTTGAATAGCTAGATTCCTTCTCTAGAAGGACGCATCAGAGAAGTTGTTGGTTTTTGAAACATGTTTTAAATTGAACTCTTATTTTAAAGGGTAGTTTGTCATTTATTTTGGCTCATATAAACTCCTAAACCACTTTTATTTTATTTTATTTTAATATTTGGAAATCAATTAGATGGCTAGAGAGTGACTTGGAAAGTGAAAGGCCGAGAGTATAATTTTTGTAAGTGAAACACTTGAAAGCATTTGGAAAAATAATTTTCGTGATAAGAAGATAGCAACTTTTTTTTCTTGTGTAGAGTTATTAGTACGAAATGCTTGTTAGTTATTTAATAATGCATTAATATATAAAACCTTCTTTTCTGCTGTTATAACTTTCCTCGAAGTTTGTTTATTTGTTTTTCCTTTCTTTCTGAGAATTTAGGCTGTGGCTATTTGTTAATTACAGGTGAGGCACCGTTTGCGGAAAGGTTATGGCATTGAAGGGGGCATTCCCGTTGTATTTTCTTTAGAGAAACCCAAAGCAAAGCTGCTTCCATTCCAAGGACCAGGTGGAGAAGCTGAAAATCCTTCAGATTATCAGGTGAGGCATTTACATGCAATACGAAAGATCTTTCTATATCTTTTGAGCAAGGCCTGCATGCATTGGGGCTACTTATGTACTTGCTTCCAAGATTAACAATTCTTGACTTGACAATGAATGAGTACTTTTTAGTGTTAAATAATTGTTGGTAGGTGTTGAAGAAAATACTAGTGTGAATAAACATTTTGAATTTTTATCATGATATCAGATAGTTCCAGGGTTCAGAGTTCGCATCATACCTGTTCTGGGCACCATCCCTGCAATCTTTGGACAGGTAATGGCCTCATACGTTGTGACACAACTGGCAGGGCTGCAAGTGGAAACAGAGCCTGTTGTCAATTTAGACATCGATCATTATAGGTTGCTTCATCAGCGACTCATTGAACACGAGGAATCATTGTATGGCACCGCCATGCAAGTCCAGGTGTGAAGGCTTTGATTAATTTTGCATGGGTTATTTTGAAGTATCCATTGTACTCCTTTTGGATTTGTTTGGATGATGCTAGTTTCTAGGTTGATTCCGAGTTGGCATTTTATGAATTCCGCAAGTCCAGTTTTGAGCAAGCCTAATTTTGATTGATTTTATGAAAAAGTACTTTAATTATTAAAAATTCAGCATAAAGACCTTAATAACAAATCACTAAGATGTTTGGTTCTACACATTTAGAAATGAAAGTGCTTTTCAGTCAAGCATCGAGTCTAATATCACTCTACAAATGCTCTGTCACAAAGTCTTTAAAGTACTTTGTTGATATGCCTTGAATCTGATAAGAAGCGCTCCGAACGACCAAGTACGGGGGTCTATTATGTTAGGGTTAAGAGTGGTGCCATATATAAACCCTCTTAACCTATTTTTGCCCTAATCTCTGTATTCTGAATACTCCTCTCATAATAAGATCGTTCTCTCCCTTTGCCCGTGGATGTAGCTAACACACCGTTAGTGAACCACGTATATCTGTGTATTGTTTTCTCTTCGCCCTTTCATTCTTGTTCGTTGGTTACTGTTTTTCTGTCATAACATACTTTTTGACTTTTCTCAAAAGCACTTTCATGTTTCTCCTAAACAAAACTTGTCCTTAATTTGGACATTGATCATGATGAGAGTGAGGAGTACTTTTATTCTAGTAATTAACTTTTCGGTTTCCATGTCTGAATGAAAGTATCCTCTTCAATATATAACATCTTTTTGTTGACACTCTATCATTCTATTGTTGTTGGATTCCAATAGTTAGGATTCTAATGAAGTATCAGTGTATTCTGATGCCATTGACTGTAGGTAGATGTTGAAGAAGTGATGTTTGTCGCAAAAGAGCTCTGGCATGGAAGAAGCGCGAGAGACCAATCTGCTAAAGATGTCGGACGGGGAATGTGGCGAGCTATTAATGAGTTAATGCTTGTGAGGTATGAATAGCTTTTCTGTTAATGTTGCCTAATATGCAACAATATTTGTTGTGGATTTAATGAAAGGATTTGGCCCACACTTTTTCTCGTTCCTTTGAACAAGGACATATAATTATAAACATATACTTTTTTTTTTTTTTTGTTTTTGATAAACCATGCTTTCATTGAGGGCGAACAGAAAAACCAGCCCACAAAAAAAGAGAGGCCCCTTAGACGAAGGGGCTCCAATCCAAAATTGGAATAATTACAAAATAAACGCGTCACCGAAGAGAAACCTTATATTTAACAAGGGACCAAACCTCAGTTTAGTCCCTCTCCAACCCTCTAAAAGTCCTACTGTTTCTCTCACCCCAAAGTCCCCAAATAAGAGATAAACATATACTTTTACTCGAATTCTATAAAATTGCATTGATAACGTCCATGAGATATGGGGCTGCATGTCCTGGTAAGGTTCTAGATTTTTTTTGAATAACGAGTGATATAATTTAATACTAACAGAAAATGAAGATTGCAATAAGTTACTTGGATTTGTTCAGATGCTCACTCTCTAGTGCTGACACTTTTTTTTTGTGTACAAATTAGGTGGGACCGAGCAAAACCAGCGTCCGTGACTAACTTAATTCTTTTGAAATTTAAAGAGGTAATTCGACTTTACTCGTTAGATGAATTTCTGGGAATTATGCTGAAGAAAGTTTCGCCGTTATTTTTCTCATGTCATTCCTTTTATCTTTCCGTTTAAAGTCATTTTACTTTCTTCACAAATTACGTATTTCGTTTAAAAATTATATACTGTCTATCAATTCTTGCTTGGACTTTTTTTTTTTTTACTTAAACTAAACATTTCTTGTCTACTGTTAGTAACTTCTGGTTTTCTTCTTTTCCCCTCGGGAAAATTTGGAGGGAAGTTTGCCATTTTTTATTTTTTATTTTTTATTTTTTTTAAAGCTTAAATTACAAATTTAGTCGCTGAACGTCGAGGACCTTGAAGTTTATATCTATTTAGTAACTTTCAAAATTTTTTCAAAAAAAAAAAAAGTAACTTTCAAAATTACAAATTTAGTCCCTCAGCTTTTTACATTTTTTTTTTAATTCATGGATCTACTAGACACAAAATTAAAATTTTAGGATTCCATTAGACATAGAATTCAAATTTATATCTAATAGATCAATTAATTTTTAAAAGTTTTGTCAGGACCTATTAGGCAAAAAATTGCGAAGTTTAGGAAATTATTAGACACTTTTGACAATGCAGAAACCAAATAGACACAAACCTCAAAGTTCAGGGACTAAACTTGTAATTTAACCAAAAAACGAAAGAGGCGAGTTGTTTACAAACGGTTGCTCTGTTTTTCATGATTCTTTACTCTTGTGAAGTGTAAAAAGCAACCATGGTCCTCTCACCACCCACTCTGTCGTATCATATTTCATTTTTGCCCATATAGTTAAAAAAAAAGTTTCGTTTTGGTCCTTCAAACATCTAAATGTTTGTTTTGGTCCCTATTGGTTTCATCTTGATCCTAAAAGTACCGATTGTGAGTCAATAGTAGATGGGAAATTGAGTTTAACCTTGTGGTTATTTGATGCAGGCTGATGAACATGAGTCATCAACCCTAGATGACATAAAGGAAAAAGAACCTGAATTCTTCACGAGGGTAACTTCTATTTTGAAGCGATCTGAGATGGATTTTTCCTTGTGAAAAAGTACACTTCTTTTTTGTAATAGATAAGGATATAGTTTTCATCTCCAATATTATCAGTTTTTTCTTTTTCTTTTTTCGTTTTAGTTCAACATACGGAGGTTAGTAGTGCCTTAATTGTTAAGCTTAGATCGGTCCAATATTATCAATTTTACAGTTGACTAAAATTTTCGTCTAGTCTGTTATTTTTTGAGATTCCGAAATATAATTTTGCCAGAGTGAGCATAGTTGGCATGTACCGTTGACCAAGAGGGAAGATGTTCGAATCCCCAACCCTCAAATGTTGTTGAATAAAAAAAAAATAGTTTTCTACCATCTGTTCAATTTAGGAGTTCATGGTACTTTTCCTAGTTTTCAAACACAAAATTGTAATCCAAA

mRNA sequence

ATGTTCTTAGCTCTTATTCATATGTCTATTGTGATTAGGAACATTCAGTTCTTTGGCCTTAGCTCACAGAAGAATGTGACTGCATCTTATGTTGTGGTAATTGGTCTTGGTGGTGTTGGGAGTCATGCTGCTATGATGCTTCTACGATCAGGGGTTGGCAGGCTTCTCCTTGTTGACTTTGACCAGGTGTCACTTTCTTCTCTAAATCGACATGCCGTTGCAACAAGAGCAGATGTAGGTGTCCCAAAAGCTCAGTGCCTGAAGGAGCACTTTCTGTCCATCTTTCCAGAGTGTCAAGTAGAAGCAAAAGTGCTATTATATGATGCAACATCTGAAGAAGAAATTCTTTGTGGAAAACCTGATTTTGTTTTGGATTGCATTGACAATATTGATACAAAGGTATCACTTCTTGCTGCATGTGTACGTAGGGGTTTGAAAGTTTTATCAGCAACAGGAGCCGGAGCTCGAGCTGACCCAACAAGAATTCGTGTGGCTGATTTAAGAGAGTCAACAAATGACCCATTATCTCGAGCTGTGAGGCACCGTTTGCGGAAAGGTTATGGCATTGAAGGGGGCATTCCCGTTGTATTTTCTTTAGAGAAACCCAAAGCAAAGCTGCTTCCATTCCAAGGACCAGGTGGAGAAGCTGAAAATCCTTCAGATTATCAGATAGTTCCAGGGTTCAGAGTTCGCATCATACCTGTTCTGGGCACCATCCCTGCAATCTTTGGACAGGTAATGGCCTCATACGTTGTGACACAACTGGCAGGGCTGCAAGTGGAAACAGAGCCTGTTGTCAATTTAGACATCGATCATTATAGGTTGCTTCATCAGCGACTCATTGAACACGAGGAATCATTGTATGGCACCGCCATGCAAGTCCAGGTAGATGTTGAAGAAGTGATGTTTGTCGCAAAAGAGCTCTGGCATGGAAGAAGCGCGAGAGACCAATCTGCTAAAGATGTCGGACGGGGAATGTGGCGAGCTATTAATGAGTTAATGCTTGTGAGGTGGGACCGAGCAAAACCAGCGTCCGTGACTAACTTAATTCTTTTGAAATTTAAAGAGGCTGATGAACATGAGTCATCAACCCTAGATGACATAAAGGAAAAAGAACCTGAATTCTTCACGAGGGTAACTTCTATTTTGAAGCGATCTGAGATGGATTTTTCCTTGTGA

Coding sequence (CDS)

ATGTTCTTAGCTCTTATTCATATGTCTATTGTGATTAGGAACATTCAGTTCTTTGGCCTTAGCTCACAGAAGAATGTGACTGCATCTTATGTTGTGGTAATTGGTCTTGGTGGTGTTGGGAGTCATGCTGCTATGATGCTTCTACGATCAGGGGTTGGCAGGCTTCTCCTTGTTGACTTTGACCAGGTGTCACTTTCTTCTCTAAATCGACATGCCGTTGCAACAAGAGCAGATGTAGGTGTCCCAAAAGCTCAGTGCCTGAAGGAGCACTTTCTGTCCATCTTTCCAGAGTGTCAAGTAGAAGCAAAAGTGCTATTATATGATGCAACATCTGAAGAAGAAATTCTTTGTGGAAAACCTGATTTTGTTTTGGATTGCATTGACAATATTGATACAAAGGTATCACTTCTTGCTGCATGTGTACGTAGGGGTTTGAAAGTTTTATCAGCAACAGGAGCCGGAGCTCGAGCTGACCCAACAAGAATTCGTGTGGCTGATTTAAGAGAGTCAACAAATGACCCATTATCTCGAGCTGTGAGGCACCGTTTGCGGAAAGGTTATGGCATTGAAGGGGGCATTCCCGTTGTATTTTCTTTAGAGAAACCCAAAGCAAAGCTGCTTCCATTCCAAGGACCAGGTGGAGAAGCTGAAAATCCTTCAGATTATCAGATAGTTCCAGGGTTCAGAGTTCGCATCATACCTGTTCTGGGCACCATCCCTGCAATCTTTGGACAGGTAATGGCCTCATACGTTGTGACACAACTGGCAGGGCTGCAAGTGGAAACAGAGCCTGTTGTCAATTTAGACATCGATCATTATAGGTTGCTTCATCAGCGACTCATTGAACACGAGGAATCATTGTATGGCACCGCCATGCAAGTCCAGGTAGATGTTGAAGAAGTGATGTTTGTCGCAAAAGAGCTCTGGCATGGAAGAAGCGCGAGAGACCAATCTGCTAAAGATGTCGGACGGGGAATGTGGCGAGCTATTAATGAGTTAATGCTTGTGAGGTGGGACCGAGCAAAACCAGCGTCCGTGACTAACTTAATTCTTTTGAAATTTAAAGAGGCTGATGAACATGAGTCATCAACCCTAGATGACATAAAGGAAAAAGAACCTGAATTCTTCACGAGGGTAACTTCTATTTTGAAGCGATCTGAGATGGATTTTTCCTTGTGA

Protein sequence

MFLALIHMSIVIRNIQFFGLSSQKNVTASYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLSSLNRHAVATRADVGVPKAQCLKEHFLSIFPECQVEAKVLLYDATSEEEILCGKPDFVLDCIDNIDTKVSLLAACVRRGLKVLSATGAGARADPTRIRVADLRESTNDPLSRAVRHRLRKGYGIEGGIPVVFSLEKPKAKLLPFQGPGGEAENPSDYQIVPGFRVRIIPVLGTIPAIFGQVMASYVVTQLAGLQVETEPVVNLDIDHYRLLHQRLIEHEESLYGTAMQVQVDVEEVMFVAKELWHGRSARDQSAKDVGRGMWRAINELMLVRWDRAKPASVTNLILLKFKEADEHESSTLDDIKEKEPEFFTRVTSILKRSEMDFSL
Homology
BLAST of Spg039061 vs. NCBI nr
Match: XP_008438195.1 (PREDICTED: tRNA threonylcarbamoyladenosine dehydratase isoform X1 [Cucumis melo])

HSP 1 Score: 723.8 bits (1867), Expect = 8.1e-205
Identity = 368/386 (95.34%), Postives = 379/386 (98.19%), Query Frame = 0

Query: 7   HMSIVIRNIQFFGLSSQKNVTASYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLS 66
           ++ +V+RNIQFFGLSSQKNVTASYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLS
Sbjct: 86  YVPVVLRNIQFFGLSSQKNVTASYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLS 145

Query: 67  SLNRHAVATRADVGVPKAQCLKEHFLSIFPECQVEAKVLLYDATSEEEILCGKPDFVLDC 126
           SLNRHAVATRADVG+PKAQCLKEHFLSIFPEC+VEAKVLLYDATSEEEIL GKPDFVLDC
Sbjct: 146 SLNRHAVATRADVGIPKAQCLKEHFLSIFPECRVEAKVLLYDATSEEEILSGKPDFVLDC 205

Query: 127 IDNIDTKVSLLAACVRRGLKVLSATGAGARADPTRIRVADLRESTNDPLSRAVRHRLRKG 186
           IDNIDTKVSLLAACVRRGLKVLSATGAGARADPTRIRVADLRESTNDPLSRAVRHRLRKG
Sbjct: 206 IDNIDTKVSLLAACVRRGLKVLSATGAGARADPTRIRVADLRESTNDPLSRAVRHRLRKG 265

Query: 187 YGIEGGIPVVFSLEKPKAKLLPFQGPGGEAENPSDYQIVPGFRVRIIPVLGTIPAIFGQV 246
           YGIEGGIPVVFSLEKPKAKLLPFQGP GEAENPSDYQIVPGFRVRIIPVLGTIPAIFGQV
Sbjct: 266 YGIEGGIPVVFSLEKPKAKLLPFQGPSGEAENPSDYQIVPGFRVRIIPVLGTIPAIFGQV 325

Query: 247 MASYVVTQLAGLQVETEPVVNLDIDHYRLLHQRLIEHEESLYGTAMQVQVDVEEVMFVAK 306
           MASYVVTQLAGLQVETEPVVNLD DHYRLLHQRLIEHEESLYGTAMQVQVDVEEVMFVAK
Sbjct: 326 MASYVVTQLAGLQVETEPVVNLDTDHYRLLHQRLIEHEESLYGTAMQVQVDVEEVMFVAK 385

Query: 307 ELWHGRSARDQSAKDVGRGMWRAINELMLVRWDRAKPASVTNLILLKFKEADEHESSTLD 366
           ELWHGRSARDQSAKDVGRGMWRAINELMLVRWDRAKPASV+NLILLKFKEADEHESSTLD
Sbjct: 386 ELWHGRSARDQSAKDVGRGMWRAINELMLVRWDRAKPASVSNLILLKFKEADEHESSTLD 445

Query: 367 DIKEKEPEFFTRVTSILKRSEMDFSL 393
           DIKE EPEFF RVT+ LKR+E++FSL
Sbjct: 446 DIKENEPEFFGRVTATLKRAEVEFSL 471

BLAST of Spg039061 vs. NCBI nr
Match: XP_038893153.1 (tRNA threonylcarbamoyladenosine dehydratase isoform X1 [Benincasa hispida])

HSP 1 Score: 723.0 bits (1865), Expect = 1.4e-204
Identity = 369/380 (97.11%), Postives = 373/380 (98.16%), Query Frame = 0

Query: 13  RNIQFFGLSSQKNVTASYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLSSLNRHA 72
           RNIQFFGLSSQKNVTASYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLSSLNRHA
Sbjct: 85  RNIQFFGLSSQKNVTASYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLSSLNRHA 144

Query: 73  VATRADVGVPKAQCLKEHFLSIFPECQVEAKVLLYDATSEEEILCGKPDFVLDCIDNIDT 132
           VATRADVGVPKAQCLKEHFLSIFPECQVEAKVLLYDA SEEEIL GKPDFVLDCIDNIDT
Sbjct: 145 VATRADVGVPKAQCLKEHFLSIFPECQVEAKVLLYDAASEEEILSGKPDFVLDCIDNIDT 204

Query: 133 KVSLLAACVRRGLKVLSATGAGARADPTRIRVADLRESTNDPLSRAVRHRLRKGYGIEGG 192
           KVSLLAACVRRGLKVLSATGAGARADPTRIRVADLRESTNDPLSRAVRHRLRKGYGIEGG
Sbjct: 205 KVSLLAACVRRGLKVLSATGAGARADPTRIRVADLRESTNDPLSRAVRHRLRKGYGIEGG 264

Query: 193 IPVVFSLEKPKAKLLPFQGPGGEAENPSDYQIVPGFRVRIIPVLGTIPAIFGQVMASYVV 252
           IPVVFSLEKPKAKLLPFQGP GEAENPSDYQIVPGFRVRIIPVLGTIPAIFGQVMASYVV
Sbjct: 265 IPVVFSLEKPKAKLLPFQGPSGEAENPSDYQIVPGFRVRIIPVLGTIPAIFGQVMASYVV 324

Query: 253 TQLAGLQVETEPVVNLDIDHYRLLHQRLIEHEESLYGTAMQVQVDVEEVMFVAKELWHGR 312
           TQLAGLQVETEPVVNLD DHYRLLHQRLIEHEESLYGTAMQVQVDVEEVMFVAKELWHGR
Sbjct: 325 TQLAGLQVETEPVVNLDTDHYRLLHQRLIEHEESLYGTAMQVQVDVEEVMFVAKELWHGR 384

Query: 313 SARDQSAKDVGRGMWRAINELMLVRWDRAKPASVTNLILLKFKEADEHESSTLDDIKEKE 372
           SARDQSAKDVGRGMWRAINELMLVRWDRAKPASV NLILLKFKEADEHESSTLDDIKEKE
Sbjct: 385 SARDQSAKDVGRGMWRAINELMLVRWDRAKPASVANLILLKFKEADEHESSTLDDIKEKE 444

Query: 373 PEFFTRVTSILKRSEMDFSL 393
           PEFF +V +ILKR+EM+FSL
Sbjct: 445 PEFFRKVNAILKRAEMEFSL 464

BLAST of Spg039061 vs. NCBI nr
Match: XP_038893168.1 (tRNA threonylcarbamoyladenosine dehydratase isoform X3 [Benincasa hispida])

HSP 1 Score: 723.0 bits (1865), Expect = 1.4e-204
Identity = 369/380 (97.11%), Postives = 373/380 (98.16%), Query Frame = 0

Query: 13  RNIQFFGLSSQKNVTASYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLSSLNRHA 72
           RNIQFFGLSSQKNVTASYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLSSLNRHA
Sbjct: 36  RNIQFFGLSSQKNVTASYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLSSLNRHA 95

Query: 73  VATRADVGVPKAQCLKEHFLSIFPECQVEAKVLLYDATSEEEILCGKPDFVLDCIDNIDT 132
           VATRADVGVPKAQCLKEHFLSIFPECQVEAKVLLYDA SEEEIL GKPDFVLDCIDNIDT
Sbjct: 96  VATRADVGVPKAQCLKEHFLSIFPECQVEAKVLLYDAASEEEILSGKPDFVLDCIDNIDT 155

Query: 133 KVSLLAACVRRGLKVLSATGAGARADPTRIRVADLRESTNDPLSRAVRHRLRKGYGIEGG 192
           KVSLLAACVRRGLKVLSATGAGARADPTRIRVADLRESTNDPLSRAVRHRLRKGYGIEGG
Sbjct: 156 KVSLLAACVRRGLKVLSATGAGARADPTRIRVADLRESTNDPLSRAVRHRLRKGYGIEGG 215

Query: 193 IPVVFSLEKPKAKLLPFQGPGGEAENPSDYQIVPGFRVRIIPVLGTIPAIFGQVMASYVV 252
           IPVVFSLEKPKAKLLPFQGP GEAENPSDYQIVPGFRVRIIPVLGTIPAIFGQVMASYVV
Sbjct: 216 IPVVFSLEKPKAKLLPFQGPSGEAENPSDYQIVPGFRVRIIPVLGTIPAIFGQVMASYVV 275

Query: 253 TQLAGLQVETEPVVNLDIDHYRLLHQRLIEHEESLYGTAMQVQVDVEEVMFVAKELWHGR 312
           TQLAGLQVETEPVVNLD DHYRLLHQRLIEHEESLYGTAMQVQVDVEEVMFVAKELWHGR
Sbjct: 276 TQLAGLQVETEPVVNLDTDHYRLLHQRLIEHEESLYGTAMQVQVDVEEVMFVAKELWHGR 335

Query: 313 SARDQSAKDVGRGMWRAINELMLVRWDRAKPASVTNLILLKFKEADEHESSTLDDIKEKE 372
           SARDQSAKDVGRGMWRAINELMLVRWDRAKPASV NLILLKFKEADEHESSTLDDIKEKE
Sbjct: 336 SARDQSAKDVGRGMWRAINELMLVRWDRAKPASVANLILLKFKEADEHESSTLDDIKEKE 395

Query: 373 PEFFTRVTSILKRSEMDFSL 393
           PEFF +V +ILKR+EM+FSL
Sbjct: 396 PEFFRKVNAILKRAEMEFSL 415

BLAST of Spg039061 vs. NCBI nr
Match: XP_022972105.1 (tRNA threonylcarbamoyladenosine dehydratase isoform X1 [Cucurbita maxima])

HSP 1 Score: 721.5 bits (1861), Expect = 4.0e-204
Identity = 366/380 (96.32%), Postives = 374/380 (98.42%), Query Frame = 0

Query: 13  RNIQFFGLSSQKNVTASYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLSSLNRHA 72
           RNIQFFGLS+QKNVTASYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLSSLNRHA
Sbjct: 85  RNIQFFGLSAQKNVTASYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLSSLNRHA 144

Query: 73  VATRADVGVPKAQCLKEHFLSIFPECQVEAKVLLYDATSEEEILCGKPDFVLDCIDNIDT 132
           VATRADVGVPKAQCLKEHFLSIFPECQVEAKVLLYDATSEEEIL GKPDFVLDCIDNIDT
Sbjct: 145 VATRADVGVPKAQCLKEHFLSIFPECQVEAKVLLYDATSEEEILSGKPDFVLDCIDNIDT 204

Query: 133 KVSLLAACVRRGLKVLSATGAGARADPTRIRVADLRESTNDPLSRAVRHRLRKGYGIEGG 192
           KVSLLAACVRRGLKVLSATGAGARADPTRIRVADLRESTNDPLSRAVRHRLRKGYGIEGG
Sbjct: 205 KVSLLAACVRRGLKVLSATGAGARADPTRIRVADLRESTNDPLSRAVRHRLRKGYGIEGG 264

Query: 193 IPVVFSLEKPKAKLLPFQGPGGEAENPSDYQIVPGFRVRIIPVLGTIPAIFGQVMASYVV 252
           IPVVFSLEKPKAKLLPFQGPGGEAENPSDYQIVPGFRVRIIPVLGTIPAIFGQVMASYVV
Sbjct: 265 IPVVFSLEKPKAKLLPFQGPGGEAENPSDYQIVPGFRVRIIPVLGTIPAIFGQVMASYVV 324

Query: 253 TQLAGLQVETEPVVNLDIDHYRLLHQRLIEHEESLYGTAMQVQVDVEEVMFVAKELWHGR 312
           TQ+AGLQVETEPVVNLDI+HYRLLHQRLIEHEESLYGTAMQVQVDVEEVMFVAKELWHGR
Sbjct: 325 TQIAGLQVETEPVVNLDIEHYRLLHQRLIEHEESLYGTAMQVQVDVEEVMFVAKELWHGR 384

Query: 313 SARDQSAKDVGRGMWRAINELMLVRWDRAKPASVTNLILLKFKEADEHESSTLDDIKEKE 372
           SARDQ AKDVGRGMWRAINELMLVRWDRAKPASV NLILL+FKEADEHES+TLDDIKE E
Sbjct: 385 SARDQFAKDVGRGMWRAINELMLVRWDRAKPASVANLILLRFKEADEHESATLDDIKENE 444

Query: 373 PEFFTRVTSILKRSEMDFSL 393
           PEFFTRVT +LKR+EM+F L
Sbjct: 445 PEFFTRVTGVLKRAEMEFFL 464

BLAST of Spg039061 vs. NCBI nr
Match: XP_022932714.1 (tRNA threonylcarbamoyladenosine dehydratase isoform X2 [Cucurbita moschata])

HSP 1 Score: 721.5 bits (1861), Expect = 4.0e-204
Identity = 366/378 (96.83%), Postives = 373/378 (98.68%), Query Frame = 0

Query: 13  RNIQFFGLSSQKNVTASYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLSSLNRHA 72
           RNIQFFGLS+QKNVTASYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLSSLNRHA
Sbjct: 36  RNIQFFGLSAQKNVTASYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLSSLNRHA 95

Query: 73  VATRADVGVPKAQCLKEHFLSIFPECQVEAKVLLYDATSEEEILCGKPDFVLDCIDNIDT 132
           VATRADVGVPKAQCLKEHFLSIFPECQVEAKVLLYDATSEEEIL GKPDFVLDCIDNIDT
Sbjct: 96  VATRADVGVPKAQCLKEHFLSIFPECQVEAKVLLYDATSEEEILSGKPDFVLDCIDNIDT 155

Query: 133 KVSLLAACVRRGLKVLSATGAGARADPTRIRVADLRESTNDPLSRAVRHRLRKGYGIEGG 192
           KVSLLAACVRRGLKVLSATGAGARADPTRIRVADLRESTNDPLSRAVRHRLRKGYGIEGG
Sbjct: 156 KVSLLAACVRRGLKVLSATGAGARADPTRIRVADLRESTNDPLSRAVRHRLRKGYGIEGG 215

Query: 193 IPVVFSLEKPKAKLLPFQGPGGEAENPSDYQIVPGFRVRIIPVLGTIPAIFGQVMASYVV 252
           IPVVFSLEKPKAKLLPFQGPGGEAENPSDYQIVPGFRVRIIPVLGTIPAIFGQVMASYVV
Sbjct: 216 IPVVFSLEKPKAKLLPFQGPGGEAENPSDYQIVPGFRVRIIPVLGTIPAIFGQVMASYVV 275

Query: 253 TQLAGLQVETEPVVNLDIDHYRLLHQRLIEHEESLYGTAMQVQVDVEEVMFVAKELWHGR 312
           TQLAGLQVETEPVVNLDI+HYRLLHQRLIEHEESLYGTAMQVQVDVEEVMFVAKELWHGR
Sbjct: 276 TQLAGLQVETEPVVNLDIEHYRLLHQRLIEHEESLYGTAMQVQVDVEEVMFVAKELWHGR 335

Query: 313 SARDQSAKDVGRGMWRAINELMLVRWDRAKPASVTNLILLKFKEADEHESSTLDDIKEKE 372
           SARDQ AKDVGRGMWRAINELMLVRWDRAKPASV NLILL+FKEADEHES+TLDDIKE E
Sbjct: 336 SARDQFAKDVGRGMWRAINELMLVRWDRAKPASVANLILLRFKEADEHESATLDDIKENE 395

Query: 373 PEFFTRVTSILKRSEMDF 391
           PEFFTRVT +LKR+EM+F
Sbjct: 396 PEFFTRVTGVLKRAEMEF 413

BLAST of Spg039061 vs. ExPASy Swiss-Prot
Match: O13861 (tRNA threonylcarbamoyladenosine dehydratase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC1A6.10 PE=3 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 1.9e-63
Identity = 140/356 (39.33%), Postives = 204/356 (57.30%), Query Frame = 0

Query: 13  RNIQFFGLSSQKNVTASYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLSSLNRHA 72
           RN  FFG    + +  S+V+V+G GGVGS    ML RSGV ++ +VDFDQVSLSSLNRH+
Sbjct: 111 RNYAFFGEDGMERLRNSFVIVVGCGGVGSWVINMLARSGVQKIRIVDFDQVSLSSLNRHS 170

Query: 73  VATRADVGVPKAQCLKEHFLSIFPECQVEAKVLLYDATSEEEILCGKPDFVLDCIDNIDT 132
           +AT  DVG PK   +K+      P  +V+A+  L++  S +++L G PDFV+D IDNI T
Sbjct: 171 IATLQDVGTPKTLAIKKAIKKFAPWIEVDARNALFNPDSADDLLSGNPDFVIDAIDNIQT 230

Query: 133 KVSLLAACVRRGLKVLSATGAGARADPTRIRVADLRESTNDPLSRAVRHRLRKGYGIEGG 192
           KV LL+ C    L V+++TG+  ++DPTR+ +AD+  ++ DPLSRA R RLR   GI  G
Sbjct: 231 KVDLLSYCYNHKLPVIASTGSACKSDPTRVNIADISATSEDPLSRATRRRLRL-LGIMEG 290

Query: 193 IPVVFSLEKP---KAKLLPFQGPGGEAENPSDYQIVPGFRVRIIPVLGTIPAIFGQVMAS 252
           IPVVFS EKP   KA LLP      E  +  +   +P FR RI+PV+G +P IFG  +A+
Sbjct: 291 IPVVFSTEKPDPRKASLLPLSEEEFEKGDVDELSALPEFRARILPVIGPMPGIFGLTIAT 350

Query: 253 YVVTQLAGLQVETEPVVNLDIDHYRLLHQRLIEHEESLYGTAMQVQVDVEEVMFVAKELW 312
           YV+T +A  +   +P+  +          + +  E    G  +    +  E+ ++ +E++
Sbjct: 351 YVLTSIA--KYPMDPISTMTRPRLYEEAVKRLHAEARKAGVNLDKTFNASEMSYIIEEVY 410

Query: 313 HGRSARDQSAKDVGRGMWRAINELMLVRWDRAKPASVTNLILLKFKEADEHESSTL 366
            GRSA                 ++ +VRW+   P   TNL+ +   EA  HE + L
Sbjct: 411 VGRSALPPHES----------QKVTVVRWNPQLPFDHTNLVAMTRNEARYHEDNVL 453

BLAST of Spg039061 vs. ExPASy Swiss-Prot
Match: P36101 (tRNA threonylcarbamoyladenosine dehydratase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TCD2 PE=1 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 1.3e-61
Identity = 138/377 (36.60%), Postives = 216/377 (57.29%), Query Frame = 0

Query: 14  NIQFFGLSSQKNVTASYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLSSLNRHAV 73
           N++F G  + + ++  YVVV+G GGVGS     L+RSG  ++ +VDFDQVSLSSLNRH+ 
Sbjct: 72  NVEFLGEDTIEKLSNQYVVVVGAGGVGSWVVNSLVRSGCRKIRVVDFDQVSLSSLNRHSC 131

Query: 74  ATRADVGVPKAQCLKEHFLSIFPECQVEAKVLLYDATSEEEILCGK--PDFVLDCIDNID 133
           A   DVG PK +CL+ H   I P C+++    L+   + E +  G   PDF++DCIDNID
Sbjct: 132 AILNDVGTPKVECLRRHMREIAPWCEIDPINELWTLQNGERLTLGNGTPDFIVDCIDNID 191

Query: 134 TKVSLLAACVRRGLKVLSATGAGARADPTRIRVADLRESTNDPLSRAVRHRLRKGYGIEG 193
           TKV LL      G+KV+S+ GA A++DPT++ V DL  +  DPL+R VR +L+K  GI  
Sbjct: 192 TKVDLLEFAYNHGIKVISSMGASAKSDPTKLNVGDLATTEEDPLARVVRRKLKK-RGILS 251

Query: 194 GIPVVFSLEKP---KAKLLPFQGPGGEAENPSDYQIVPGFRVRIIPVLGTIPAIFGQVMA 253
           GIPVVFS EKP   KAKLLP      E     +   +  FRVRI+PVLGT+P++FG  + 
Sbjct: 252 GIPVVFSAEKPDPKKAKLLPLPDEEYERGKVDELSALKDFRVRILPVLGTMPSLFGLTIT 311

Query: 254 SYVVTQLAGLQVETEPVVNLDIDHYRLLHQRLIEHEESLYGTAMQVQVDVEEVMFVAKEL 313
           +++++ ++   +E     N  I  Y  ++Q L      +   + ++ + +++V ++ +E+
Sbjct: 312 TWILSNISDKPLEPVEGKN-RIKVYDGIYQSLAGQMSRVGIPSQRIPLALKDVSYLVEEV 371

Query: 314 WHGRSARDQSAKDVGRGMWRAINELMLVRWDRAKPASVTNLILLKFKEADEHESSTLDDI 373
           + G+S     +             L L +WD +KP S+ N+++L   E   HE   L   
Sbjct: 372 FKGKSPISGIS-----------TRLTLTKWDPSKPISLQNVVVLTKNEQKVHEDRVLKGK 431

Query: 374 KEKEPEFFTRVTSILKR 386
           +  +  +  +V  ++ +
Sbjct: 432 ESLQDVYDAKVLKLVSQ 435

BLAST of Spg039061 vs. ExPASy Swiss-Prot
Match: P38756 (tRNA threonylcarbamoyladenosine dehydratase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TCD1 PE=1 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 3.9e-45
Identity = 117/384 (30.47%), Postives = 204/384 (53.12%), Query Frame = 0

Query: 13  RNIQFFGLSSQKNVTASYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLSSLNRHA 72
           RN  F G    + +   Y+V++G G VGS    ML+RSG  +++++D + +S+ SLN H 
Sbjct: 59  RNYAFLGEEGMRKIKEQYIVIVGAGEVGSWVCTMLIRSGCQKIMIIDPENISIDSLNTHC 118

Query: 73  VATRADVGVPKAQCLKEHFLSIFPECQVEAKVLLYDATSEEEILCG---KPDFVLDCIDN 132
            A  +D+G PK QCLKEH   I P  +++A+   +   +  +++      P F++DC+DN
Sbjct: 119 CAVLSDIGKPKVQCLKEHLSKIAPWSEIKARAKAWTKENSHDLIFADGESPTFIVDCLDN 178

Query: 133 IDTKVSLLAACVRRGLKVLSATGAGARADPTRIRVADLRESTNDPLSRAVRHRLRKGYGI 192
           +++KV LL       + V+S+ G   ++DPTR+ + D+  +  DP+SR VR +LRK  GI
Sbjct: 179 LESKVDLLEYAHHNKIDVISSMGVATKSDPTRVSINDISMTEFDPISRCVRRKLRK-RGI 238

Query: 193 EGGIPVVFSLEKPKAKLLPFQGPGGEAENPSDYQIVPGFRVRIIPVLGTIPAIFGQVMAS 252
             GI VVFS E    +      P  + E+ +    V    +R +P LGT+P IFG  +A+
Sbjct: 239 ATGISVVFSNEMLDPRRDDILSP-IDCEHRA-INAVRDEALRHLPELGTMPGIFGLSIAT 298

Query: 253 YVVTQLAGLQVETEPVVN------LDIDHYRLLHQRLIEHEE--SLYGTAMQVQVDVEEV 312
           +++T+++G  ++   V N        ++ ++    RL E++E  SL G        +EEV
Sbjct: 299 WILTKVSGYPMKENEVKNRLKFYDSILETFQKQMARLNENKERSSLLG--------LEEV 358

Query: 313 MFVAKELWHGRSARDQSAKDVGRGMWRAINELMLVRWDRAKPASVTNLILLKFKEADEHE 372
            ++ +E++ G+S     +            +L L +W+  K  S+TN++L+  +E + HE
Sbjct: 359 GYIVEEMFRGKSPISGYS-----------TKLALTKWEANKEISLTNVVLMTKEEQEIHE 418

Query: 373 SSTLDDIKEKEPEFFTRVTSILKR 386
              L D ++    +   V   ++R
Sbjct: 419 KRILLDGEKLTAVYSEEVLDFIER 420

BLAST of Spg039061 vs. ExPASy Swiss-Prot
Match: O32037 (tRNA threonylcarbamoyladenosine dehydratase OS=Bacillus subtilis (strain 168) OX=224308 GN=tcdA PE=3 SV=3)

HSP 1 Score: 141.0 bits (354), Expect = 2.9e-32
Identity = 77/184 (41.85%), Postives = 114/184 (61.96%), Query Frame = 0

Query: 19  GLSSQKNVTASYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLSSLNRHAVATRAD 78
           GL + KN T   V V+G+GGVGS AA  L RSGVGR+LLVD D V ++++NR   A  + 
Sbjct: 16  GLETLKNST---VAVLGVGGVGSFAAEALARSGVGRILLVDKDDVDITNVNRQLHALLST 75

Query: 79  VGVPKAQCLKEHFLSIFPECQVEAKVLLYDATSEEEILCGKPDFVLDCIDNIDTKVSLLA 138
           VG PK   +K     I PEC+V A  + Y   + E+      D+V+D  D I  K+ L+ 
Sbjct: 76  VGQPKVDLMKARIADINPECEVIALKMFYTEETYEQFFDYGLDYVIDASDTICYKIHLMK 135

Query: 139 ACVRRGLKVLSATGAGARADPTRIRVADLRESTNDPLSRAVRHRLRKGYGIEGGIPVVFS 198
            C++R + ++S+ GA  + DPTR ++AD+ ++  DP+++ VR +LRK  GI+ G+ V+FS
Sbjct: 136 ECLKRDIPLISSMGAANKTDPTRFQIADISKTHTDPIAKVVRTKLRK-EGIKKGVQVIFS 195

Query: 199 LEKP 203
            E P
Sbjct: 196 DESP 195

BLAST of Spg039061 vs. ExPASy Swiss-Prot
Match: Q57097 (tRNA threonylcarbamoyladenosine dehydratase OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=tcdA PE=3 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 3.1e-26
Identity = 64/172 (37.21%), Postives = 96/172 (55.81%), Query Frame = 0

Query: 29  SYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLSSLNRHAVATRADVGVPKAQCLK 88
           +++ VIG+GGVGS     L RSG+G L L+D D + ++++NR   A    +G  K + + 
Sbjct: 34  AHICVIGIGGVGSWVVEALARSGIGELTLIDMDDICVTNINRQLPAMSGTIGKLKTEVMS 93

Query: 89  EHFLSIFPECQVEAKVLLYDATSEEEILCGKPDFVLDCIDNIDTKVSLLAACVRRGLKVL 148
           E    I PEC V          ++ + L    D+V+D IDN+ TK SL+A C R  + V+
Sbjct: 94  ERVKLINPECTVNIIDDFISPENQSDYLNRGYDYVIDAIDNVKTKASLIAYCKRNKINVI 153

Query: 149 SATGAGARADPTRIRVADLRESTNDPLSRAVRHRLRKGYGIEGGIPVVFSLE 201
           +  GAG + DPT+I++ADL ++  DPL   VR  LRK Y         FS++
Sbjct: 154 TIGGAGGQTDPTQIQIADLSKTIQDPLLAKVRSVLRKDYNFSQNPKRKFSID 205

BLAST of Spg039061 vs. ExPASy TrEMBL
Match: A0A1S3AWF9 (tRNA threonylcarbamoyladenosine dehydratase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483373 PE=4 SV=1)

HSP 1 Score: 723.8 bits (1867), Expect = 3.9e-205
Identity = 368/386 (95.34%), Postives = 379/386 (98.19%), Query Frame = 0

Query: 7   HMSIVIRNIQFFGLSSQKNVTASYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLS 66
           ++ +V+RNIQFFGLSSQKNVTASYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLS
Sbjct: 86  YVPVVLRNIQFFGLSSQKNVTASYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLS 145

Query: 67  SLNRHAVATRADVGVPKAQCLKEHFLSIFPECQVEAKVLLYDATSEEEILCGKPDFVLDC 126
           SLNRHAVATRADVG+PKAQCLKEHFLSIFPEC+VEAKVLLYDATSEEEIL GKPDFVLDC
Sbjct: 146 SLNRHAVATRADVGIPKAQCLKEHFLSIFPECRVEAKVLLYDATSEEEILSGKPDFVLDC 205

Query: 127 IDNIDTKVSLLAACVRRGLKVLSATGAGARADPTRIRVADLRESTNDPLSRAVRHRLRKG 186
           IDNIDTKVSLLAACVRRGLKVLSATGAGARADPTRIRVADLRESTNDPLSRAVRHRLRKG
Sbjct: 206 IDNIDTKVSLLAACVRRGLKVLSATGAGARADPTRIRVADLRESTNDPLSRAVRHRLRKG 265

Query: 187 YGIEGGIPVVFSLEKPKAKLLPFQGPGGEAENPSDYQIVPGFRVRIIPVLGTIPAIFGQV 246
           YGIEGGIPVVFSLEKPKAKLLPFQGP GEAENPSDYQIVPGFRVRIIPVLGTIPAIFGQV
Sbjct: 266 YGIEGGIPVVFSLEKPKAKLLPFQGPSGEAENPSDYQIVPGFRVRIIPVLGTIPAIFGQV 325

Query: 247 MASYVVTQLAGLQVETEPVVNLDIDHYRLLHQRLIEHEESLYGTAMQVQVDVEEVMFVAK 306
           MASYVVTQLAGLQVETEPVVNLD DHYRLLHQRLIEHEESLYGTAMQVQVDVEEVMFVAK
Sbjct: 326 MASYVVTQLAGLQVETEPVVNLDTDHYRLLHQRLIEHEESLYGTAMQVQVDVEEVMFVAK 385

Query: 307 ELWHGRSARDQSAKDVGRGMWRAINELMLVRWDRAKPASVTNLILLKFKEADEHESSTLD 366
           ELWHGRSARDQSAKDVGRGMWRAINELMLVRWDRAKPASV+NLILLKFKEADEHESSTLD
Sbjct: 386 ELWHGRSARDQSAKDVGRGMWRAINELMLVRWDRAKPASVSNLILLKFKEADEHESSTLD 445

Query: 367 DIKEKEPEFFTRVTSILKRSEMDFSL 393
           DIKE EPEFF RVT+ LKR+E++FSL
Sbjct: 446 DIKENEPEFFGRVTATLKRAEVEFSL 471

BLAST of Spg039061 vs. ExPASy TrEMBL
Match: A0A6J1I7L9 (tRNA threonylcarbamoyladenosine dehydratase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111470738 PE=4 SV=1)

HSP 1 Score: 721.5 bits (1861), Expect = 1.9e-204
Identity = 366/380 (96.32%), Postives = 374/380 (98.42%), Query Frame = 0

Query: 13  RNIQFFGLSSQKNVTASYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLSSLNRHA 72
           RNIQFFGLS+QKNVTASYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLSSLNRHA
Sbjct: 85  RNIQFFGLSAQKNVTASYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLSSLNRHA 144

Query: 73  VATRADVGVPKAQCLKEHFLSIFPECQVEAKVLLYDATSEEEILCGKPDFVLDCIDNIDT 132
           VATRADVGVPKAQCLKEHFLSIFPECQVEAKVLLYDATSEEEIL GKPDFVLDCIDNIDT
Sbjct: 145 VATRADVGVPKAQCLKEHFLSIFPECQVEAKVLLYDATSEEEILSGKPDFVLDCIDNIDT 204

Query: 133 KVSLLAACVRRGLKVLSATGAGARADPTRIRVADLRESTNDPLSRAVRHRLRKGYGIEGG 192
           KVSLLAACVRRGLKVLSATGAGARADPTRIRVADLRESTNDPLSRAVRHRLRKGYGIEGG
Sbjct: 205 KVSLLAACVRRGLKVLSATGAGARADPTRIRVADLRESTNDPLSRAVRHRLRKGYGIEGG 264

Query: 193 IPVVFSLEKPKAKLLPFQGPGGEAENPSDYQIVPGFRVRIIPVLGTIPAIFGQVMASYVV 252
           IPVVFSLEKPKAKLLPFQGPGGEAENPSDYQIVPGFRVRIIPVLGTIPAIFGQVMASYVV
Sbjct: 265 IPVVFSLEKPKAKLLPFQGPGGEAENPSDYQIVPGFRVRIIPVLGTIPAIFGQVMASYVV 324

Query: 253 TQLAGLQVETEPVVNLDIDHYRLLHQRLIEHEESLYGTAMQVQVDVEEVMFVAKELWHGR 312
           TQ+AGLQVETEPVVNLDI+HYRLLHQRLIEHEESLYGTAMQVQVDVEEVMFVAKELWHGR
Sbjct: 325 TQIAGLQVETEPVVNLDIEHYRLLHQRLIEHEESLYGTAMQVQVDVEEVMFVAKELWHGR 384

Query: 313 SARDQSAKDVGRGMWRAINELMLVRWDRAKPASVTNLILLKFKEADEHESSTLDDIKEKE 372
           SARDQ AKDVGRGMWRAINELMLVRWDRAKPASV NLILL+FKEADEHES+TLDDIKE E
Sbjct: 385 SARDQFAKDVGRGMWRAINELMLVRWDRAKPASVANLILLRFKEADEHESATLDDIKENE 444

Query: 373 PEFFTRVTSILKRSEMDFSL 393
           PEFFTRVT +LKR+EM+F L
Sbjct: 445 PEFFTRVTGVLKRAEMEFFL 464

BLAST of Spg039061 vs. ExPASy TrEMBL
Match: A0A6J1I520 (tRNA threonylcarbamoyladenosine dehydratase isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111470738 PE=4 SV=1)

HSP 1 Score: 721.5 bits (1861), Expect = 1.9e-204
Identity = 366/380 (96.32%), Postives = 374/380 (98.42%), Query Frame = 0

Query: 13  RNIQFFGLSSQKNVTASYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLSSLNRHA 72
           RNIQFFGLS+QKNVTASYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLSSLNRHA
Sbjct: 36  RNIQFFGLSAQKNVTASYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLSSLNRHA 95

Query: 73  VATRADVGVPKAQCLKEHFLSIFPECQVEAKVLLYDATSEEEILCGKPDFVLDCIDNIDT 132
           VATRADVGVPKAQCLKEHFLSIFPECQVEAKVLLYDATSEEEIL GKPDFVLDCIDNIDT
Sbjct: 96  VATRADVGVPKAQCLKEHFLSIFPECQVEAKVLLYDATSEEEILSGKPDFVLDCIDNIDT 155

Query: 133 KVSLLAACVRRGLKVLSATGAGARADPTRIRVADLRESTNDPLSRAVRHRLRKGYGIEGG 192
           KVSLLAACVRRGLKVLSATGAGARADPTRIRVADLRESTNDPLSRAVRHRLRKGYGIEGG
Sbjct: 156 KVSLLAACVRRGLKVLSATGAGARADPTRIRVADLRESTNDPLSRAVRHRLRKGYGIEGG 215

Query: 193 IPVVFSLEKPKAKLLPFQGPGGEAENPSDYQIVPGFRVRIIPVLGTIPAIFGQVMASYVV 252
           IPVVFSLEKPKAKLLPFQGPGGEAENPSDYQIVPGFRVRIIPVLGTIPAIFGQVMASYVV
Sbjct: 216 IPVVFSLEKPKAKLLPFQGPGGEAENPSDYQIVPGFRVRIIPVLGTIPAIFGQVMASYVV 275

Query: 253 TQLAGLQVETEPVVNLDIDHYRLLHQRLIEHEESLYGTAMQVQVDVEEVMFVAKELWHGR 312
           TQ+AGLQVETEPVVNLDI+HYRLLHQRLIEHEESLYGTAMQVQVDVEEVMFVAKELWHGR
Sbjct: 276 TQIAGLQVETEPVVNLDIEHYRLLHQRLIEHEESLYGTAMQVQVDVEEVMFVAKELWHGR 335

Query: 313 SARDQSAKDVGRGMWRAINELMLVRWDRAKPASVTNLILLKFKEADEHESSTLDDIKEKE 372
           SARDQ AKDVGRGMWRAINELMLVRWDRAKPASV NLILL+FKEADEHES+TLDDIKE E
Sbjct: 336 SARDQFAKDVGRGMWRAINELMLVRWDRAKPASVANLILLRFKEADEHESATLDDIKENE 395

Query: 373 PEFFTRVTSILKRSEMDFSL 393
           PEFFTRVT +LKR+EM+F L
Sbjct: 396 PEFFTRVTGVLKRAEMEFFL 415

BLAST of Spg039061 vs. ExPASy TrEMBL
Match: A0A6J1EXT1 (tRNA threonylcarbamoyladenosine dehydratase isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111439179 PE=4 SV=1)

HSP 1 Score: 721.5 bits (1861), Expect = 1.9e-204
Identity = 366/378 (96.83%), Postives = 373/378 (98.68%), Query Frame = 0

Query: 13  RNIQFFGLSSQKNVTASYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLSSLNRHA 72
           RNIQFFGLS+QKNVTASYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLSSLNRHA
Sbjct: 36  RNIQFFGLSAQKNVTASYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLSSLNRHA 95

Query: 73  VATRADVGVPKAQCLKEHFLSIFPECQVEAKVLLYDATSEEEILCGKPDFVLDCIDNIDT 132
           VATRADVGVPKAQCLKEHFLSIFPECQVEAKVLLYDATSEEEIL GKPDFVLDCIDNIDT
Sbjct: 96  VATRADVGVPKAQCLKEHFLSIFPECQVEAKVLLYDATSEEEILSGKPDFVLDCIDNIDT 155

Query: 133 KVSLLAACVRRGLKVLSATGAGARADPTRIRVADLRESTNDPLSRAVRHRLRKGYGIEGG 192
           KVSLLAACVRRGLKVLSATGAGARADPTRIRVADLRESTNDPLSRAVRHRLRKGYGIEGG
Sbjct: 156 KVSLLAACVRRGLKVLSATGAGARADPTRIRVADLRESTNDPLSRAVRHRLRKGYGIEGG 215

Query: 193 IPVVFSLEKPKAKLLPFQGPGGEAENPSDYQIVPGFRVRIIPVLGTIPAIFGQVMASYVV 252
           IPVVFSLEKPKAKLLPFQGPGGEAENPSDYQIVPGFRVRIIPVLGTIPAIFGQVMASYVV
Sbjct: 216 IPVVFSLEKPKAKLLPFQGPGGEAENPSDYQIVPGFRVRIIPVLGTIPAIFGQVMASYVV 275

Query: 253 TQLAGLQVETEPVVNLDIDHYRLLHQRLIEHEESLYGTAMQVQVDVEEVMFVAKELWHGR 312
           TQLAGLQVETEPVVNLDI+HYRLLHQRLIEHEESLYGTAMQVQVDVEEVMFVAKELWHGR
Sbjct: 276 TQLAGLQVETEPVVNLDIEHYRLLHQRLIEHEESLYGTAMQVQVDVEEVMFVAKELWHGR 335

Query: 313 SARDQSAKDVGRGMWRAINELMLVRWDRAKPASVTNLILLKFKEADEHESSTLDDIKEKE 372
           SARDQ AKDVGRGMWRAINELMLVRWDRAKPASV NLILL+FKEADEHES+TLDDIKE E
Sbjct: 336 SARDQFAKDVGRGMWRAINELMLVRWDRAKPASVANLILLRFKEADEHESATLDDIKENE 395

Query: 373 PEFFTRVTSILKRSEMDF 391
           PEFFTRVT +LKR+EM+F
Sbjct: 396 PEFFTRVTGVLKRAEMEF 413

BLAST of Spg039061 vs. ExPASy TrEMBL
Match: A0A6J1F2I9 (tRNA threonylcarbamoyladenosine dehydratase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111439179 PE=4 SV=1)

HSP 1 Score: 721.5 bits (1861), Expect = 1.9e-204
Identity = 366/378 (96.83%), Postives = 373/378 (98.68%), Query Frame = 0

Query: 13  RNIQFFGLSSQKNVTASYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLSSLNRHA 72
           RNIQFFGLS+QKNVTASYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLSSLNRHA
Sbjct: 85  RNIQFFGLSAQKNVTASYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLSSLNRHA 144

Query: 73  VATRADVGVPKAQCLKEHFLSIFPECQVEAKVLLYDATSEEEILCGKPDFVLDCIDNIDT 132
           VATRADVGVPKAQCLKEHFLSIFPECQVEAKVLLYDATSEEEIL GKPDFVLDCIDNIDT
Sbjct: 145 VATRADVGVPKAQCLKEHFLSIFPECQVEAKVLLYDATSEEEILSGKPDFVLDCIDNIDT 204

Query: 133 KVSLLAACVRRGLKVLSATGAGARADPTRIRVADLRESTNDPLSRAVRHRLRKGYGIEGG 192
           KVSLLAACVRRGLKVLSATGAGARADPTRIRVADLRESTNDPLSRAVRHRLRKGYGIEGG
Sbjct: 205 KVSLLAACVRRGLKVLSATGAGARADPTRIRVADLRESTNDPLSRAVRHRLRKGYGIEGG 264

Query: 193 IPVVFSLEKPKAKLLPFQGPGGEAENPSDYQIVPGFRVRIIPVLGTIPAIFGQVMASYVV 252
           IPVVFSLEKPKAKLLPFQGPGGEAENPSDYQIVPGFRVRIIPVLGTIPAIFGQVMASYVV
Sbjct: 265 IPVVFSLEKPKAKLLPFQGPGGEAENPSDYQIVPGFRVRIIPVLGTIPAIFGQVMASYVV 324

Query: 253 TQLAGLQVETEPVVNLDIDHYRLLHQRLIEHEESLYGTAMQVQVDVEEVMFVAKELWHGR 312
           TQLAGLQVETEPVVNLDI+HYRLLHQRLIEHEESLYGTAMQVQVDVEEVMFVAKELWHGR
Sbjct: 325 TQLAGLQVETEPVVNLDIEHYRLLHQRLIEHEESLYGTAMQVQVDVEEVMFVAKELWHGR 384

Query: 313 SARDQSAKDVGRGMWRAINELMLVRWDRAKPASVTNLILLKFKEADEHESSTLDDIKEKE 372
           SARDQ AKDVGRGMWRAINELMLVRWDRAKPASV NLILL+FKEADEHES+TLDDIKE E
Sbjct: 385 SARDQFAKDVGRGMWRAINELMLVRWDRAKPASVANLILLRFKEADEHESATLDDIKENE 444

Query: 373 PEFFTRVTSILKRSEMDF 391
           PEFFTRVT +LKR+EM+F
Sbjct: 445 PEFFTRVTGVLKRAEMEF 462

BLAST of Spg039061 vs. TAIR 10
Match: AT5G37530.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 639.0 bits (1647), Expect = 2.4e-183
Identity = 310/378 (82.01%), Postives = 352/378 (93.12%), Query Frame = 0

Query: 13  RNIQFFGLSSQKNVTASYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLSSLNRHA 72
           RNIQFFGL SQ  VT SYVVVIGLGGVGSHAA MLLRSGVG+LLLVDFDQVSLSSLNRHA
Sbjct: 78  RNIQFFGLESQHKVTGSYVVVIGLGGVGSHAASMLLRSGVGKLLLVDFDQVSLSSLNRHA 137

Query: 73  VATRADVGVPKAQCLKEHFLSIFPECQVEAKVLLYDATSEEEILCGKPDFVLDCIDNIDT 132
           VATRADVG+PKA CLK+HF SIFPEC +EAKV+LYD++SEEEIL GKPDFVLDCIDNIDT
Sbjct: 138 VATRADVGIPKAMCLKKHFSSIFPECHIEAKVMLYDSSSEEEILSGKPDFVLDCIDNIDT 197

Query: 133 KVSLLAACVRRGLKVLSATGAGARADPTRIRVADLRESTNDPLSRAVRHRLRKGYGIEGG 192
           KV LLAACV+RGLKVLSATGAGARADPTRIRVAD+REST DPLSR+VRHRLR+ +GIEGG
Sbjct: 198 KVGLLAACVKRGLKVLSATGAGARADPTRIRVADIRESTIDPLSRSVRHRLRRKHGIEGG 257

Query: 193 IPVVFSLEKPKAKLLPFQGPGGEAENPSDYQIVPGFRVRIIPVLGTIPAIFGQVMASYVV 252
           IPVVFSLEKPKAKLLPF+G  GE ENP DYQ+VPGFRVRIIPVLGTIPAIFGQ+MASYV+
Sbjct: 258 IPVVFSLEKPKAKLLPFEGTNGEDENPLDYQVVPGFRVRIIPVLGTIPAIFGQIMASYVI 317

Query: 253 TQLAGLQVETEPVVNLDIDHYRLLHQRLIEHEESLYGTAMQVQVDVEEVMFVAKELWHGR 312
           TQLAG+QV+ EP+VNLD+DHYRLLHQRLIEHEE++YGT+ +V+VDVEEVM++ KELWHGR
Sbjct: 318 TQLAGVQVQMEPIVNLDLDHYRLLHQRLIEHEETVYGTSAEVEVDVEEVMYIVKELWHGR 377

Query: 313 SARDQSAKDVGRGMWRAINELMLVRWDRAKPASVTNLILLKFKEADEHESSTLDDIKEKE 372
           SARD++AKDVGRGMWRA+NELMLVRWD  KPA+V+NL+LLKFKEADEHE+ TL+++KE E
Sbjct: 378 SARDETAKDVGRGMWRAMNELMLVRWDAKKPATVSNLVLLKFKEADEHETKTLEEVKESE 437

Query: 373 PEFFTRVTSILKRSEMDF 391
            EFF RV+ +LK++E+DF
Sbjct: 438 TEFFERVSCVLKKAELDF 455

BLAST of Spg039061 vs. TAIR 10
Match: AT5G37530.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 639.0 bits (1647), Expect = 2.4e-183
Identity = 310/378 (82.01%), Postives = 352/378 (93.12%), Query Frame = 0

Query: 13  RNIQFFGLSSQKNVTASYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLSSLNRHA 72
           RNIQFFGL SQ  VT SYVVVIGLGGVGSHAA MLLRSGVG+LLLVDFDQVSLSSLNRHA
Sbjct: 77  RNIQFFGLESQHKVTGSYVVVIGLGGVGSHAASMLLRSGVGKLLLVDFDQVSLSSLNRHA 136

Query: 73  VATRADVGVPKAQCLKEHFLSIFPECQVEAKVLLYDATSEEEILCGKPDFVLDCIDNIDT 132
           VATRADVG+PKA CLK+HF SIFPEC +EAKV+LYD++SEEEIL GKPDFVLDCIDNIDT
Sbjct: 137 VATRADVGIPKAMCLKKHFSSIFPECHIEAKVMLYDSSSEEEILSGKPDFVLDCIDNIDT 196

Query: 133 KVSLLAACVRRGLKVLSATGAGARADPTRIRVADLRESTNDPLSRAVRHRLRKGYGIEGG 192
           KV LLAACV+RGLKVLSATGAGARADPTRIRVAD+REST DPLSR+VRHRLR+ +GIEGG
Sbjct: 197 KVGLLAACVKRGLKVLSATGAGARADPTRIRVADIRESTIDPLSRSVRHRLRRKHGIEGG 256

Query: 193 IPVVFSLEKPKAKLLPFQGPGGEAENPSDYQIVPGFRVRIIPVLGTIPAIFGQVMASYVV 252
           IPVVFSLEKPKAKLLPF+G  GE ENP DYQ+VPGFRVRIIPVLGTIPAIFGQ+MASYV+
Sbjct: 257 IPVVFSLEKPKAKLLPFEGTNGEDENPLDYQVVPGFRVRIIPVLGTIPAIFGQIMASYVI 316

Query: 253 TQLAGLQVETEPVVNLDIDHYRLLHQRLIEHEESLYGTAMQVQVDVEEVMFVAKELWHGR 312
           TQLAG+QV+ EP+VNLD+DHYRLLHQRLIEHEE++YGT+ +V+VDVEEVM++ KELWHGR
Sbjct: 317 TQLAGVQVQMEPIVNLDLDHYRLLHQRLIEHEETVYGTSAEVEVDVEEVMYIVKELWHGR 376

Query: 313 SARDQSAKDVGRGMWRAINELMLVRWDRAKPASVTNLILLKFKEADEHESSTLDDIKEKE 372
           SARD++AKDVGRGMWRA+NELMLVRWD  KPA+V+NL+LLKFKEADEHE+ TL+++KE E
Sbjct: 377 SARDETAKDVGRGMWRAMNELMLVRWDAKKPATVSNLVLLKFKEADEHETKTLEEVKESE 436

Query: 373 PEFFTRVTSILKRSEMDF 391
            EFF RV+ +LK++E+DF
Sbjct: 437 TEFFERVSCVLKKAELDF 454

BLAST of Spg039061 vs. TAIR 10
Match: AT5G55130.1 (co-factor for nitrate, reductase and xanthine dehydrogenase 5 )

HSP 1 Score: 69.7 bits (169), Expect = 5.9e-12
Identity = 45/143 (31.47%), Postives = 72/143 (50.35%), Query Frame = 0

Query: 18  FGLSSQKNVTASYVVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLSSLNRHAVATRA 77
           F +  Q N+  S V+VIG GG+GS A + L   GVG+L ++D D V L++++R  + T A
Sbjct: 81  FAVEGQSNLLKSSVLVIGAGGLGSPALLYLAACGVGQLGIIDHDVVELNNMHRQIIHTEA 140

Query: 78  DVGVPKAQCLKEHFLSIFPECQVEAKVLLYDATSEEEILCGKPDFVLDCIDNIDTKVSLL 137
            +G PK +       SI    +V+  V     ++  EIL  + D ++D  DN  ++  + 
Sbjct: 141 FIGHPKVKSAAAACRSINSTIKVDEYVEALRTSNALEIL-SQYDIIVDATDNPPSRYMIS 200

Query: 138 AACVRRGLKVLSATGAGARADPT 161
             CV  G  ++S    G     T
Sbjct: 201 DCCVLLGKPLVSGAALGMEGQLT 222

BLAST of Spg039061 vs. TAIR 10
Match: AT1G05350.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 62.8 bits (151), Expect = 7.2e-10
Identity = 41/126 (32.54%), Postives = 66/126 (52.38%), Query Frame = 0

Query: 31  VVVIGLGGVGSHAAMMLLRSGVGRLLLVDFDQVSLSSLNRHAVATRAD-VGVPKAQCLKE 90
           V ++G+GGVGS AA ML R G+GRLLL D+D V L+++NR  +  R D VG+ K     +
Sbjct: 85  VAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDTVELANMNR--LFFRPDQVGMTKTDAAVQ 144

Query: 91  HFLSIFPECQVEAKVL---------LYDATSEEEILCGKP------DFVLDCIDNIDTKV 141
               I P+  +E+  +          + ++   +  C         D VL C+DN + ++
Sbjct: 145 TLAEINPDVVLESFTMNITTVQGFETFTSSLTNKSFCPSKEGGSGVDLVLSCVDNYEARM 204

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008438195.18.1e-20595.34PREDICTED: tRNA threonylcarbamoyladenosine dehydratase isoform X1 [Cucumis melo][more]
XP_038893153.11.4e-20497.11tRNA threonylcarbamoyladenosine dehydratase isoform X1 [Benincasa hispida][more]
XP_038893168.11.4e-20497.11tRNA threonylcarbamoyladenosine dehydratase isoform X3 [Benincasa hispida][more]
XP_022972105.14.0e-20496.32tRNA threonylcarbamoyladenosine dehydratase isoform X1 [Cucurbita maxima][more]
XP_022932714.14.0e-20496.83tRNA threonylcarbamoyladenosine dehydratase isoform X2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
O138611.9e-6339.33tRNA threonylcarbamoyladenosine dehydratase OS=Schizosaccharomyces pombe (strain... [more]
P361011.3e-6136.60tRNA threonylcarbamoyladenosine dehydratase 2 OS=Saccharomyces cerevisiae (strai... [more]
P387563.9e-4530.47tRNA threonylcarbamoyladenosine dehydratase 1 OS=Saccharomyces cerevisiae (strai... [more]
O320372.9e-3241.85tRNA threonylcarbamoyladenosine dehydratase OS=Bacillus subtilis (strain 168) OX... [more]
Q570973.1e-2637.21tRNA threonylcarbamoyladenosine dehydratase OS=Haemophilus influenzae (strain AT... [more]
Match NameE-valueIdentityDescription
A0A1S3AWF93.9e-20595.34tRNA threonylcarbamoyladenosine dehydratase isoform X1 OS=Cucumis melo OX=3656 G... [more]
A0A6J1I7L91.9e-20496.32tRNA threonylcarbamoyladenosine dehydratase isoform X1 OS=Cucurbita maxima OX=36... [more]
A0A6J1I5201.9e-20496.32tRNA threonylcarbamoyladenosine dehydratase isoform X2 OS=Cucurbita maxima OX=36... [more]
A0A6J1EXT11.9e-20496.83tRNA threonylcarbamoyladenosine dehydratase isoform X2 OS=Cucurbita moschata OX=... [more]
A0A6J1F2I91.9e-20496.83tRNA threonylcarbamoyladenosine dehydratase isoform X1 OS=Cucurbita moschata OX=... [more]
Match NameE-valueIdentityDescription
AT5G37530.12.4e-18382.01NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G37530.22.4e-18382.01NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G55130.15.9e-1231.47co-factor for nitrate, reductase and xanthine dehydrogenase 5 [more]
AT1G05350.17.2e-1032.54NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.720coord: 9..263
e-value: 5.8E-66
score: 224.7
NoneNo IPR availablePANTHERPTHR43267:SF2TRNA THREONYLCARBAMOYLADENOSINE DEHYDRATASE 1-RELATEDcoord: 11..390
NoneNo IPR availablePANTHERPTHR43267TRNA THREONYLCARBAMOYLADENOSINE DEHYDRATASEcoord: 11..390
NoneNo IPR availableCDDcd00755YgdL_likecoord: 37..252
e-value: 2.1489E-89
score: 267.549
IPR000594THIF-type NAD/FAD binding foldPFAMPF00899ThiFcoord: 13..263
e-value: 1.7E-39
score: 135.7
IPR035985Ubiquitin-activating enzymeSUPERFAMILY69572Activating enzymes of the ubiquitin-like proteinscoord: 13..261

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg039061.1Spg039061.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0061504 cyclic threonylcarbamoyladenosine biosynthetic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0061503 tRNA threonylcarbamoyladenosine dehydratase
molecular_function GO:0008641 ubiquitin-like modifier activating enzyme activity