Spg037334 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg037334
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionPyridoxal 5'-phosphate synthase subunit PdxT
Locationscaffold8: 827973 .. 830280 (+)
RNA-Seq ExpressionSpg037334
SyntenySpg037334
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTTTTTCCCTTTTTTCACTCTTCCTTTCCTTTCTTTCCTTTCACACGCAGTACGCACGCCTCCTTCGACCTTCACCCTTTCCCTCCCAATCGCAAAAGCCGCTCCCAGCCTCTCTCTGTTCAACCACCGTTGCTCGCACTTTCATTCGCGCATCGCAGCAGCCAAGCCAACAACGTGGATTTTCGTTTTACTTTCTCCTTTTTTTTTTTTTTTGAAACTCTGGTTTTACTTTGTCATTTTTTTTCCTTTTCAGTTTTCACGCCTCTTAAATCGAGATTTAAATTGGAACATTACTTCTGCTCCGCTTGTCTGCTTGATTAGATCTAAAACTCTTTGTGATAATGTTTGCAAAAAAAGATTCCTCTGCCGCAGAGAGGCTTTTCCATTCAGCGCCACTCGAAACGAGAAGTTCGAAGTGTTCAATCAACAGACGCTTCTGATTCTCTCTTTCACTCGCTCCCACTGCCTGCTTATTCGCCGCCGGAGCCGGAGCTCCGCGGCTTCTAGGGCCACTGAACGCTCCATTTCACACATGGCGATCGTCGGCGTTCTAGCTCTGCAGGGATCTTTTAACGAGCACATCGCAGGTATCTTCTTCTTCTCTATCGCTAGCTCATTTCGTAGTGGGCGTTTCTATGGATGAATACACTATGTTGTTTTACTGTGATGAGTTTTCGCCCTTAATTGATTGTGACGCGCAGCTTTGAGAAGGCTCGGAGTGAAGGGCGTAGAGATAAGGAAGCCCAAGCAGCTTCTTAATGTAACGTCTTTAATTATTCCTGGGGGAGAAAGCACCACCATGGGCAAACTTGCGGAGCTCCATAATTTGGTTTATGCCTCTTTCCTCTAGACGTTGCTGCATCTGTAATCGTCTTTAAAGAGTTTCTTTAGTTTCTCCAAAAAACTTTTCGAACACGCGATGAGCTTTTGCCATCTTCAGTTAGCATACATGCCTGCTTGTCTATAATTACATAAAAGCGAGTTAAAGATACTACATTCAATCGTGTAATAAACAACAATGTTATCTTTAAAACTTGTCTCTATGGTTTGCTTGGTAATGCTATTTGTCAAGAGTCGACGTCTGAGCACTATTCCCACTGTAGCTTTTGCTTGCATTTATATCTTTCTCTGTATTGTATTCTCCCAGAACTTCATTTATTTGGATGGGATTTCACTCACGTAATCTCCTCGATTATCTCTAGTTTGGTTAGGTTAGGTAATGTAAATATTTTCTCCCTGAGATTCGTTTCCTATCTTCTTGTATTTATTGAGGTTTAAACCTCCTTTGCTGAATAACAGAGATTGATTACTTTTCTCCTCAAAACTTAATTCTGTTATCTCGGATAACACATACCAACATCCCAAATGTATGAAATAATCGAATGTGTTAGTTTGGATGGGCTCAATACTTGGAATTAGAATCGAGTGAAGTATTTATATCGTCGTATTTTTGTATATTTGCAGTTTCCTGCTTTGCGAGAGTTCGTTAGGATGGGGAAGCCTGTCTGGGGCACCTGTGCAGGGCTTATCTTTTTGGCAAACAAAGCTACTGGTAGATTTCCTTCCTTTTTCCCTGAACATCTCAACTTCGAATGTTTTCCCCACCTCCAAAACACTAATAAAATGGTTTAAATGTGTTCTAAACTTGATATGGATACATGTATTTTCAGGACAGAAAACCGGAGGACAGGAATTTGTTGGAGGCCTAGATTGTACTGTTCATAGAAACTTCTTTGGCAGTCAGGTAACTCTCGCTGGTTTCTCATGTTGGGTTGTGCTATCAAGATGCATGAGCAATAAGTGGAAAACAAAAGAACCTTGGAAATTGGTACAGAACACGTATGGGATTCAAATCAGCATAAAGCCACAGGGATCTCAGGGCTTGCGAGAAAGATGCCCAATTTGATTGGCCCGTTCTGTTGTATTCTCTGTAGGATGGCGGAGGAAGACCTCGATCATATTTTATGGCACTGTGACTTTGCTCGCTCGGTTTGGAACAATTTGTTTGAGATTTTTGAGCTTCAATTTGCTAGACACAGGGATCTTAGGGAGATGATTGATGAGTTCCTTCCCCATCCTCCTTTTCGGGACCAAGGAAATTTCTTATGGCAAGCTGGGATTTGTGCTATCATTTGGGGTTTGTGGGGGGAGAGAAATAACAGAATTTTTAGAGGGAAGGAGAGTAATGCGGAGGATGTTTGGGACATTATTAAATATTCTGTCTCTTTATGGGCGTTGGTGACGCGTTTATTTTGTAATTATCCTTTAGGTCTTATTTTGTTAGATTGGCGTCCCTTTATCTAA

mRNA sequence

ATGATTTTTTCCCTTTTTTCACTCTTCCTTTCCTTTCTTTCCTTTCACACGCAGTACGCACGCCTCCTTCGACCTTCACCCTTTCCCTCCCAATCGCAAAAGCCGCTCCCAGCCTCTCTCTGTTCAACCACCGTTGCTCGCACTTTCATTCGCGCATCGCAGCAGCCAAGCCAACAACGTGGATTTTCATCTAAAACTCTTTGTGATAATGTTTGCAAAAAAAGATTCCTCTGCCGCAGAGAGGCTTTTCCATTCAGCGCCACTCGAAACGAGAAGTTCGAAGTGTTCAATCAACAGACGCTTCTGATTCTCTCTTTCACTCGCTCCCACTGCCTGCTTATTCGCCGCCGGAGCCGGAGCTCCGCGGCTTCTAGGGCCACTGAACGCTCCATTTCACACATGGCGATCGTCGGCGTTCTAGCTCTGCAGGGATCTTTTAACGAGCACATCGCAGCTTTGAGAAGGCTCGGAGTGAAGGGCGTAGAGATAAGGAAGCCCAAGCAGCTTCTTAATGTAACGTCTTTAATTATTCCTGGGGGAGAAAGCACCACCATGGGCAAACTTGCGGAGCTCCATAATTTGTTTCCTGCTTTGCGAGAGTTCGTTAGGATGGGGAAGCCTGTCTGGGGCACCTGTGCAGGGCTTATCTTTTTGGCAAACAAAGCTACTGGACAGAAAACCGGAGGACAGGAATTTGTTGGAGGCCTAGATTGTACTGTTCATAGAAACTTCTTTGGCAGTCAGGTAACTCTCGCTGGTTTCTCATGTTGGGTTGTGCTATCAAGATGCATGAGCAATAAGTGGAAAACAAAAGAACCTTGGAAATTGGTACAGAACACGATGGCGGAGGAAGACCTCGATCATATTTTATGGCACTGTGACTTTGCTCGCTCGGTTTGGAACAATTTGTTTGAGATTTTTGAGCTTCAATTTGCTAGACACAGGGATCTTAGGGAGATGATTGATGAGTTCCTTCCCCATCCTCCTTTTCGGGACCAAGGAAATTTCTTATGGCAAGCTGGGATTTGTGCTATCATTTGGGGTTTGTGGGGGGAGAGAAATAACAGAATTTTTAGAGGGAAGGAGAGTAATGCGGAGGATGTTTGGGACATTATTAAATATTCTGTCTCTTTATGGGCGTTGGTGACGCGTTTATTTTGTAATTATCCTTTAGGTCTTATTTTGTTAGATTGGCGTCCCTTTATCTAA

Coding sequence (CDS)

ATGATTTTTTCCCTTTTTTCACTCTTCCTTTCCTTTCTTTCCTTTCACACGCAGTACGCACGCCTCCTTCGACCTTCACCCTTTCCCTCCCAATCGCAAAAGCCGCTCCCAGCCTCTCTCTGTTCAACCACCGTTGCTCGCACTTTCATTCGCGCATCGCAGCAGCCAAGCCAACAACGTGGATTTTCATCTAAAACTCTTTGTGATAATGTTTGCAAAAAAAGATTCCTCTGCCGCAGAGAGGCTTTTCCATTCAGCGCCACTCGAAACGAGAAGTTCGAAGTGTTCAATCAACAGACGCTTCTGATTCTCTCTTTCACTCGCTCCCACTGCCTGCTTATTCGCCGCCGGAGCCGGAGCTCCGCGGCTTCTAGGGCCACTGAACGCTCCATTTCACACATGGCGATCGTCGGCGTTCTAGCTCTGCAGGGATCTTTTAACGAGCACATCGCAGCTTTGAGAAGGCTCGGAGTGAAGGGCGTAGAGATAAGGAAGCCCAAGCAGCTTCTTAATGTAACGTCTTTAATTATTCCTGGGGGAGAAAGCACCACCATGGGCAAACTTGCGGAGCTCCATAATTTGTTTCCTGCTTTGCGAGAGTTCGTTAGGATGGGGAAGCCTGTCTGGGGCACCTGTGCAGGGCTTATCTTTTTGGCAAACAAAGCTACTGGACAGAAAACCGGAGGACAGGAATTTGTTGGAGGCCTAGATTGTACTGTTCATAGAAACTTCTTTGGCAGTCAGGTAACTCTCGCTGGTTTCTCATGTTGGGTTGTGCTATCAAGATGCATGAGCAATAAGTGGAAAACAAAAGAACCTTGGAAATTGGTACAGAACACGATGGCGGAGGAAGACCTCGATCATATTTTATGGCACTGTGACTTTGCTCGCTCGGTTTGGAACAATTTGTTTGAGATTTTTGAGCTTCAATTTGCTAGACACAGGGATCTTAGGGAGATGATTGATGAGTTCCTTCCCCATCCTCCTTTTCGGGACCAAGGAAATTTCTTATGGCAAGCTGGGATTTGTGCTATCATTTGGGGTTTGTGGGGGGAGAGAAATAACAGAATTTTTAGAGGGAAGGAGAGTAATGCGGAGGATGTTTGGGACATTATTAAATATTCTGTCTCTTTATGGGCGTTGGTGACGCGTTTATTTTGTAATTATCCTTTAGGTCTTATTTTGTTAGATTGGCGTCCCTTTATCTAA

Protein sequence

MIFSLFSLFLSFLSFHTQYARLLRPSPFPSQSQKPLPASLCSTTVARTFIRASQQPSQQRGFSSKTLCDNVCKKRFLCRREAFPFSATRNEKFEVFNQQTLLILSFTRSHCLLIRRRSRSSAASRATERSISHMAIVGVLALQGSFNEHIAALRRLGVKGVEIRKPKQLLNVTSLIIPGGESTTMGKLAELHNLFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQEFVGGLDCTVHRNFFGSQVTLAGFSCWVVLSRCMSNKWKTKEPWKLVQNTMAEEDLDHILWHCDFARSVWNNLFEIFELQFARHRDLREMIDEFLPHPPFRDQGNFLWQAGICAIIWGLWGERNNRIFRGKESNAEDVWDIIKYSVSLWALVTRLFCNYPLGLILLDWRPFI
Homology
BLAST of Spg037334 vs. NCBI nr
Match: XP_023522767.1 (probable pyridoxal 5'-phosphate synthase subunit PDX2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 235.3 bits (599), Expect = 8.9e-58
Identity = 115/122 (94.26%), Postives = 117/122 (95.90%), Query Frame = 0

Query: 134 MAIVGVLALQGSFNEHIAALRRLGVKGVEIRKPKQLLNVTSLIIPGGESTTMGKLAELHN 193
           MAIVGVLALQGSFNEHIAALRRLGVKGVEIRKP+QLLNV SLIIPGGESTTM KLA+LHN
Sbjct: 1   MAIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLADLHN 60

Query: 194 LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQEFVGGLDCTVHRNFFGSQVTLAG 253
           LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQE VGGLDCTVHRNFFGSQVTL  
Sbjct: 61  LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQELVGGLDCTVHRNFFGSQVTLVA 120

Query: 254 FS 256
           FS
Sbjct: 121 FS 122

BLAST of Spg037334 vs. NCBI nr
Match: XP_022140743.1 (probable pyridoxal 5'-phosphate synthase subunit PDX2 [Momordica charantia])

HSP 1 Score: 226.5 bits (576), Expect = 4.1e-55
Identity = 110/116 (94.83%), Postives = 113/116 (97.41%), Query Frame = 0

Query: 134 MAIVGVLALQGSFNEHIAALRRLGVKGVEIRKPKQLLNVTSLIIPGGESTTMGKLAELHN 193
           MAIVGVLALQGSFNEHI+ALRRLGVKGVEIRKP+QLLNV SLIIPGGESTTM KLAELHN
Sbjct: 1   MAIVGVLALQGSFNEHISALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60

Query: 194 LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQEFVGGLDCTVHRNFFGSQV 250
           LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQE VGGLDCTVHRNFFGSQ+
Sbjct: 61  LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQELVGGLDCTVHRNFFGSQL 116

BLAST of Spg037334 vs. NCBI nr
Match: XP_023511885.1 (probable pyridoxal 5'-phosphate synthase subunit PDX2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 226.5 bits (576), Expect = 4.1e-55
Identity = 110/116 (94.83%), Postives = 113/116 (97.41%), Query Frame = 0

Query: 134 MAIVGVLALQGSFNEHIAALRRLGVKGVEIRKPKQLLNVTSLIIPGGESTTMGKLAELHN 193
           MAIVGVLALQGSFNEHIAALRRLGVKGVEIRKP+QLLNV SLIIPGGESTTM KLA+LHN
Sbjct: 1   MAIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLADLHN 60

Query: 194 LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQEFVGGLDCTVHRNFFGSQV 250
           LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQE VGGLDCTVHRNFFGSQ+
Sbjct: 61  LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQELVGGLDCTVHRNFFGSQL 116

BLAST of Spg037334 vs. NCBI nr
Match: XP_038900301.1 (probable pyridoxal 5'-phosphate synthase subunit PDX2 [Benincasa hispida])

HSP 1 Score: 224.9 bits (572), Expect = 1.2e-54
Identity = 110/116 (94.83%), Postives = 112/116 (96.55%), Query Frame = 0

Query: 134 MAIVGVLALQGSFNEHIAALRRLGVKGVEIRKPKQLLNVTSLIIPGGESTTMGKLAELHN 193
           MAIVGVLALQGSFNEHIAALRRLGVKGVEIRKP+QLLNV SLIIPGGESTTM KLAELHN
Sbjct: 1   MAIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60

Query: 194 LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQEFVGGLDCTVHRNFFGSQV 250
           LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQ  VGGLDCTVHRNFFGSQ+
Sbjct: 61  LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQL 116

BLAST of Spg037334 vs. NCBI nr
Match: XP_022986058.1 (probable pyridoxal 5'-phosphate synthase subunit PDX2 [Cucurbita maxima])

HSP 1 Score: 224.6 bits (571), Expect = 1.6e-54
Identity = 109/116 (93.97%), Postives = 112/116 (96.55%), Query Frame = 0

Query: 134 MAIVGVLALQGSFNEHIAALRRLGVKGVEIRKPKQLLNVTSLIIPGGESTTMGKLAELHN 193
           M IVGVLALQGSFNEHIAALRRLGVKGVEIRKP+QLLNV SLIIPGGESTTM KLA+LHN
Sbjct: 7   MPIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLADLHN 66

Query: 194 LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQEFVGGLDCTVHRNFFGSQV 250
           LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQE VGGLDCTVHRNFFGSQ+
Sbjct: 67  LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQELVGGLDCTVHRNFFGSQL 122

BLAST of Spg037334 vs. ExPASy Swiss-Prot
Match: Q8LAD0 (Probable pyridoxal 5'-phosphate synthase subunit PDX2 OS=Arabidopsis thaliana OX=3702 GN=PDX2 PE=1 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 7.6e-52
Identity = 99/113 (87.61%), Postives = 105/113 (92.92%), Query Frame = 0

Query: 137 VGVLALQGSFNEHIAALRRLGVKGVEIRKPKQLLNVTSLIIPGGESTTMGKLAELHNLFP 196
           VGVLALQGSFNEHIAALRRLGV+GVEIRK  QLL V+SLIIPGGESTTM KLAE HNLFP
Sbjct: 3   VGVLALQGSFNEHIAALRRLGVQGVEIRKADQLLTVSSLIIPGGESTTMAKLAEYHNLFP 62

Query: 197 ALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQEFVGGLDCTVHRNFFGSQV 250
           ALREFV+MGKPVWGTCAGLIFLA++A GQK GGQE VGGLDCTVHRNFFGSQ+
Sbjct: 63  ALREFVKMGKPVWGTCAGLIFLADRAVGQKEGGQELVGGLDCTVHRNFFGSQI 115

BLAST of Spg037334 vs. ExPASy Swiss-Prot
Match: B8G664 (Pyridoxal 5'-phosphate synthase subunit PdxT OS=Chloroflexus aggregans (strain MD-66 / DSM 9485) OX=326427 GN=pdxT PE=3 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 2.5e-26
Identity = 58/113 (51.33%), Postives = 76/113 (67.26%), Query Frame = 0

Query: 137 VGVLALQGSFNEHIAALRRLGVKGVEIRKPKQLLNVTSLIIPGGESTTMGKLAELHNLFP 196
           VGVLALQG F EH A LRR+GV+ +E+R P QL  V  LIIPGGESTT+G+L  ++ +  
Sbjct: 3   VGVLALQGDFREHCAVLRRIGVEPIEVRLPHQLAQVDHLIIPGGESTTIGRLLAIYQMLE 62

Query: 197 ALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQEFVGGLDCTVHRNFFGSQV 250
            +R        +WGTCAG I LAN+   QK GGQ  +G ++ T+ RN +GSQ+
Sbjct: 63  PIRTRGGCDLAIWGTCAGAILLANEVMDQKQGGQPTLGLMNLTIRRNAYGSQL 115

BLAST of Spg037334 vs. ExPASy Swiss-Prot
Match: A9WFU0 (Pyridoxal 5'-phosphate synthase subunit PdxT OS=Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl) OX=324602 GN=pdxT PE=3 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 7.2e-26
Identity = 57/113 (50.44%), Postives = 76/113 (67.26%), Query Frame = 0

Query: 137 VGVLALQGSFNEHIAALRRLGVKGVEIRKPKQLLNVTSLIIPGGESTTMGKLAELHNLFP 196
           VGVLALQG F EH A LRR+GV+ +E+R P+QL  V  LIIPGGESTT+G+L  ++ +  
Sbjct: 3   VGVLALQGDFREHCAVLRRIGVEPIEVRLPRQLEQVERLIIPGGESTTIGRLLTIYQMLE 62

Query: 197 ALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQEFVGGLDCTVHRNFFGSQV 250
            +R        +WGTCAG I LA+  T QK GGQ  +  ++ T+ RN +GSQ+
Sbjct: 63  PIRARAGRDLAIWGTCAGAILLASVVTDQKQGGQPTLSLMNLTIQRNAYGSQL 115

BLAST of Spg037334 vs. ExPASy Swiss-Prot
Match: B9LIK4 (Pyridoxal 5'-phosphate synthase subunit PdxT OS=Chloroflexus aurantiacus (strain ATCC 29364 / DSM 637 / Y-400-fl) OX=480224 GN=pdxT PE=3 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 7.2e-26
Identity = 57/113 (50.44%), Postives = 76/113 (67.26%), Query Frame = 0

Query: 137 VGVLALQGSFNEHIAALRRLGVKGVEIRKPKQLLNVTSLIIPGGESTTMGKLAELHNLFP 196
           VGVLALQG F EH A LRR+GV+ +E+R P+QL  V  LIIPGGESTT+G+L  ++ +  
Sbjct: 3   VGVLALQGDFREHCAVLRRIGVEPIEVRLPRQLEQVERLIIPGGESTTIGRLLTIYQMLE 62

Query: 197 ALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQEFVGGLDCTVHRNFFGSQV 250
            +R        +WGTCAG I LA+  T QK GGQ  +  ++ T+ RN +GSQ+
Sbjct: 63  PIRARAGRDLAIWGTCAGAILLASVVTDQKQGGQPTLSLMNLTIQRNAYGSQL 115

BLAST of Spg037334 vs. ExPASy Swiss-Prot
Match: B1W3G0 (Pyridoxal 5'-phosphate synthase subunit PdxT OS=Streptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350) OX=455632 GN=pdxT PE=3 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 6.1e-25
Identity = 60/118 (50.85%), Postives = 75/118 (63.56%), Query Frame = 0

Query: 131 ISHMAIVGVLALQGSFNEHIAALRRLGVKGVEIRKPKQLLNVTSLIIPGGESTTMGKLAE 190
           +S   ++GVLALQG   EH+ AL         +R+P++L  V  L+IPGGESTTM KLA 
Sbjct: 1   MSDTPVIGVLALQGDVREHLIALASADALARPVRRPEELAEVDGLVIPGGESTTMSKLAV 60

Query: 191 LHNLFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQEFVGGLDCTVHRNFFGSQ 249
           L  +   LRE VR G PV+GTCAG+I LA K    ++ GQE VGG+D  V RN FG Q
Sbjct: 61  LFGMMEPLRERVRAGMPVYGTCAGMILLAEKILDPRS-GQETVGGIDMIVRRNAFGRQ 117

BLAST of Spg037334 vs. ExPASy TrEMBL
Match: A0A6J1CIP7 (Glutaminase OS=Momordica charantia OX=3673 GN=LOC111011333 PE=3 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 2.0e-55
Identity = 110/116 (94.83%), Postives = 113/116 (97.41%), Query Frame = 0

Query: 134 MAIVGVLALQGSFNEHIAALRRLGVKGVEIRKPKQLLNVTSLIIPGGESTTMGKLAELHN 193
           MAIVGVLALQGSFNEHI+ALRRLGVKGVEIRKP+QLLNV SLIIPGGESTTM KLAELHN
Sbjct: 1   MAIVGVLALQGSFNEHISALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60

Query: 194 LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQEFVGGLDCTVHRNFFGSQV 250
           LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQE VGGLDCTVHRNFFGSQ+
Sbjct: 61  LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQELVGGLDCTVHRNFFGSQL 116

BLAST of Spg037334 vs. ExPASy TrEMBL
Match: A0A6J1JFE0 (Glutaminase OS=Cucurbita maxima OX=3661 GN=LOC111483918 PE=3 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 7.6e-55
Identity = 109/116 (93.97%), Postives = 112/116 (96.55%), Query Frame = 0

Query: 134 MAIVGVLALQGSFNEHIAALRRLGVKGVEIRKPKQLLNVTSLIIPGGESTTMGKLAELHN 193
           M IVGVLALQGSFNEHIAALRRLGVKGVEIRKP+QLLNV SLIIPGGESTTM KLA+LHN
Sbjct: 7   MPIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLADLHN 66

Query: 194 LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQEFVGGLDCTVHRNFFGSQV 250
           LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQE VGGLDCTVHRNFFGSQ+
Sbjct: 67  LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQELVGGLDCTVHRNFFGSQL 122

BLAST of Spg037334 vs. ExPASy TrEMBL
Match: A0A6J1FUQ8 (Glutaminase OS=Cucurbita moschata OX=3662 GN=LOC111448973 PE=3 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 1.0e-54
Identity = 108/116 (93.10%), Postives = 112/116 (96.55%), Query Frame = 0

Query: 134 MAIVGVLALQGSFNEHIAALRRLGVKGVEIRKPKQLLNVTSLIIPGGESTTMGKLAELHN 193
           M +VGVLALQGSFNEHIAALRRLGVKGVEIRKP+QLLNV SLIIPGGESTTM KLA+LHN
Sbjct: 1   MPVVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLADLHN 60

Query: 194 LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQEFVGGLDCTVHRNFFGSQV 250
           LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQE VGGLDCTVHRNFFGSQ+
Sbjct: 61  LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQELVGGLDCTVHRNFFGSQL 116

BLAST of Spg037334 vs. ExPASy TrEMBL
Match: A0A0A0KAR5 (Glutaminase OS=Cucumis sativus OX=3659 GN=Csa_6G139790 PE=3 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 1.7e-54
Identity = 109/116 (93.97%), Postives = 111/116 (95.69%), Query Frame = 0

Query: 134 MAIVGVLALQGSFNEHIAALRRLGVKGVEIRKPKQLLNVTSLIIPGGESTTMGKLAELHN 193
           M IVGVLALQGSFNEHIAALRRLGVKGVEIRKP+QLLNV SLIIPGGESTTM KLAELHN
Sbjct: 1   MTIVGVLALQGSFNEHIAALRRLGVKGVEIRKPEQLLNVASLIIPGGESTTMAKLAELHN 60

Query: 194 LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQEFVGGLDCTVHRNFFGSQV 250
           LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQ  VGGLDCTVHRNFFGSQ+
Sbjct: 61  LFPALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQGLVGGLDCTVHRNFFGSQL 116

BLAST of Spg037334 vs. ExPASy TrEMBL
Match: A0A2G2VQA9 (Glutaminase OS=Capsicum baccatum OX=33114 GN=CQW23_26967 PE=3 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 3.8e-54
Identity = 105/114 (92.11%), Postives = 112/114 (98.25%), Query Frame = 0

Query: 136 IVGVLALQGSFNEHIAALRRLGVKGVEIRKPKQLLNVTSLIIPGGESTTMGKLAELHNLF 195
           +VGVLALQGSFNEHIAAL+RLGVKGVE+RKP+QLLNV+SLIIPGGESTTM KLAELHNLF
Sbjct: 2   VVGVLALQGSFNEHIAALKRLGVKGVEVRKPEQLLNVSSLIIPGGESTTMAKLAELHNLF 61

Query: 196 PALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQEFVGGLDCTVHRNFFGSQV 250
           PALREFV+MGKPVWGTCAGLIFLANKATGQKTGGQE VGGLDCTVHRNFFGSQ+
Sbjct: 62  PALREFVQMGKPVWGTCAGLIFLANKATGQKTGGQELVGGLDCTVHRNFFGSQI 115

BLAST of Spg037334 vs. TAIR 10
Match: AT5G60540.1 (pyridoxine biosynthesis 2 )

HSP 1 Score: 206.1 bits (523), Expect = 5.4e-53
Identity = 99/113 (87.61%), Postives = 105/113 (92.92%), Query Frame = 0

Query: 137 VGVLALQGSFNEHIAALRRLGVKGVEIRKPKQLLNVTSLIIPGGESTTMGKLAELHNLFP 196
           VGVLALQGSFNEHIAALRRLGV+GVEIRK  QLL V+SLIIPGGESTTM KLAE HNLFP
Sbjct: 3   VGVLALQGSFNEHIAALRRLGVQGVEIRKADQLLTVSSLIIPGGESTTMAKLAEYHNLFP 62

Query: 197 ALREFVRMGKPVWGTCAGLIFLANKATGQKTGGQEFVGGLDCTVHRNFFGSQV 250
           ALREFV+MGKPVWGTCAGLIFLA++A GQK GGQE VGGLDCTVHRNFFGSQ+
Sbjct: 63  ALREFVKMGKPVWGTCAGLIFLADRAVGQKEGGQELVGGLDCTVHRNFFGSQI 115

BLAST of Spg037334 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 46.2 bits (108), Expect = 7.2e-05
Identity = 28/104 (26.92%), Postives = 47/104 (45.19%), Query Frame = 0

Query: 283 EEDLDHILWHCDFARSVWNNLFEIFELQFARHRDLREMIDEFLPHPPFRDQGNFLWQAG- 342
           +E ++H+L+ C FAR  W     I  +      +  + I   L        GN  W+   
Sbjct: 302 KETVNHLLFKCTFARLTW----AISSIPIPLGGEWADSIYVNLYWVFNLGNGNPQWEKAS 361

Query: 343 --ICAIIWGLWGERNNRIFRGKESNAEDVWDIIKYSVSLWALVT 384
             +  ++W LW  RN  +FRG+E NA++V    +  +  W + T
Sbjct: 362 QLVPWLLWRLWKNRNELVFRGREFNAQEVLRRAEDDLEEWRIRT 401

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023522767.18.9e-5894.26probable pyridoxal 5'-phosphate synthase subunit PDX2 [Cucurbita pepo subsp. pep... [more]
XP_022140743.14.1e-5594.83probable pyridoxal 5'-phosphate synthase subunit PDX2 [Momordica charantia][more]
XP_023511885.14.1e-5594.83probable pyridoxal 5'-phosphate synthase subunit PDX2 [Cucurbita pepo subsp. pep... [more]
XP_038900301.11.2e-5494.83probable pyridoxal 5'-phosphate synthase subunit PDX2 [Benincasa hispida][more]
XP_022986058.11.6e-5493.97probable pyridoxal 5'-phosphate synthase subunit PDX2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q8LAD07.6e-5287.61Probable pyridoxal 5'-phosphate synthase subunit PDX2 OS=Arabidopsis thaliana OX... [more]
B8G6642.5e-2651.33Pyridoxal 5'-phosphate synthase subunit PdxT OS=Chloroflexus aggregans (strain M... [more]
A9WFU07.2e-2650.44Pyridoxal 5'-phosphate synthase subunit PdxT OS=Chloroflexus aurantiacus (strain... [more]
B9LIK47.2e-2650.44Pyridoxal 5'-phosphate synthase subunit PdxT OS=Chloroflexus aurantiacus (strain... [more]
B1W3G06.1e-2550.85Pyridoxal 5'-phosphate synthase subunit PdxT OS=Streptomyces griseus subsp. gris... [more]
Match NameE-valueIdentityDescription
A0A6J1CIP72.0e-5594.83Glutaminase OS=Momordica charantia OX=3673 GN=LOC111011333 PE=3 SV=1[more]
A0A6J1JFE07.6e-5593.97Glutaminase OS=Cucurbita maxima OX=3661 GN=LOC111483918 PE=3 SV=1[more]
A0A6J1FUQ81.0e-5493.10Glutaminase OS=Cucurbita moschata OX=3662 GN=LOC111448973 PE=3 SV=1[more]
A0A0A0KAR51.7e-5493.97Glutaminase OS=Cucumis sativus OX=3659 GN=Csa_6G139790 PE=3 SV=1[more]
A0A2G2VQA93.8e-5492.11Glutaminase OS=Capsicum baccatum OX=33114 GN=CQW23_26967 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G60540.15.4e-5387.61pyridoxine biosynthesis 2 [more]
AT4G29090.17.2e-0526.92Ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029062Class I glutamine amidotransferase-likeGENE3D3.40.50.880coord: 133..278
e-value: 4.9E-45
score: 155.6
IPR029062Class I glutamine amidotransferase-likeSUPERFAMILY52317Class I glutamine amidotransferase-likecoord: 136..259
IPR002161Pyridoxal 5'-phosphate synthase subunit PdxT/SNOPFAMPF01174SNOcoord: 139..250
e-value: 1.5E-30
score: 106.4
IPR002161Pyridoxal 5'-phosphate synthase subunit PdxT/SNOTIGRFAMTIGR03800TIGR03800coord: 137..249
e-value: 3.4E-36
score: 122.9
IPR002161Pyridoxal 5'-phosphate synthase subunit PdxT/SNOPANTHERPTHR31559PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNOcoord: 135..250
IPR002161Pyridoxal 5'-phosphate synthase subunit PdxT/SNOPROSITEPS51130PDXT_SNO_2coord: 136..249
score: 36.665623
NoneNo IPR availablePANTHERPTHR31559:SF1SUBFAMILY NOT NAMEDcoord: 135..250
IPR021196PdxT/SNO family, conserved sitePROSITEPS01236PDXT_SNO_1coord: 174..184

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg037334.1Spg037334.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006541 glutamine metabolic process
biological_process GO:0042823 pyridoxal phosphate biosynthetic process
biological_process GO:0008614 pyridoxine metabolic process
biological_process GO:0042819 vitamin B6 biosynthetic process
cellular_component GO:0005829 cytosol
cellular_component GO:1903600 glutaminase complex
molecular_function GO:0004359 glutaminase activity