Spg036806 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg036806 vs. NCBI nr
Match: XP_038893761.1 (L-ascorbate oxidase homolog [Benincasa hispida])

HSP 1 Score: 1101.7 bits (2848), Expect = 0.0e+00
Identity = 520/542 (95.94%), Postives = 535/542 (98.71%), Query Frame = 0

Query: 80  MRECRVLCSLIFLVLFAVNEVNAENPYRFFTWKVTYGNIYPLGVKQQGILINGQFPGPQI 139
           MRE RVLCSLI LVLFAVNEVNA+NPYRFFTWKVTYGNIYPLGVKQQGILINGQFPGPQI
Sbjct: 1   MRENRVLCSLIILVLFAVNEVNADNPYRFFTWKVTYGNIYPLGVKQQGILINGQFPGPQI 60

Query: 140 DAVTNDNLIINVYNYLKEPFLISWNGLQQRRNSWQDGVYGTTCAIPPLRNFTYVLQAKDQ 199
           DAVTNDNLIINVYNYLKEPFLISWNGLQQRRNSWQDGVYGTTCAIPPLRNFTY LQAKDQ
Sbjct: 61  DAVTNDNLIINVYNYLKEPFLISWNGLQQRRNSWQDGVYGTTCAIPPLRNFTYTLQAKDQ 120

Query: 200 IGSYFYFPSTAFHKAAGAFGGIRIWSRPGIPVPFAPPAGDFTVLAGDWFKTNHYILRQAL 259
           IGSYFYFPSTAFHKAAGAFGGIRIWSRPGIPVPFAPPAGDFTVLAGDWFKTNHYILRQ L
Sbjct: 121 IGSYFYFPSTAFHKAAGAFGGIRIWSRPGIPVPFAPPAGDFTVLAGDWFKTNHYILRQVL 180

Query: 260 DNGQNLPSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGISTSINFRIQGHVMKLVEVE 319
           ++G+NLPSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGIS SINFRIQGHVMKLVEVE
Sbjct: 181 ESGKNLPSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGISASINFRIQGHVMKLVEVE 240

Query: 320 GSHTLQNTYSSLDVHVGQSYSVLVTADQPSKDYYVVVSSRFTSPILTTTAVLHYSNSWQK 379
           GSHTLQNTYSSLD+H+GQSYSVLVTA+QP KDYYVVVSSRFTSPILTTTAVLHYSNSWQK
Sbjct: 241 GSHTLQNTYSSLDIHLGQSYSVLVTANQPPKDYYVVVSSRFTSPILTTTAVLHYSNSWQK 300

Query: 380 VSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILANSAPII 439
           VSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILANSAP+I
Sbjct: 301 VSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILANSAPVI 360

Query: 440 NGKQRFAVNSVSFVQADTPLKLADFFKIPGVFSLNSIPTTPTWGNAYLQTSVMGTNFREY 499
           NGKQRFAVNSVSFVQADTPLKLAD+FKIPGVF+LNSIPTTPTWGNAYLQTSVMG+NFREY
Sbjct: 361 NGKQRFAVNSVSFVQADTPLKLADYFKIPGVFNLNSIPTTPTWGNAYLQTSVMGSNFREY 420

Query: 500 IEIVFQNWEDTVQSWHIDGYSFFVVGMDGGQWTPASRARYNLRDTVARCTTQVYPKSWTA 559
           IEIVFQNWEDTVQSWHI+GYSFFVVGMDGGQWTPASRARYNLRDTVARCTTQVYPK+WTA
Sbjct: 421 IEIVFQNWEDTVQSWHINGYSFFVVGMDGGQWTPASRARYNLRDTVARCTTQVYPKAWTA 480

Query: 560 IYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSLRDELPIPRNALLCGRASGRHTR 619
           IYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTS RDELPIPRNAL+CG+ASGRHTR
Sbjct: 481 IYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSFRDELPIPRNALVCGKASGRHTR 540

Query: 620 PL 622
           PL
Sbjct: 541 PL 542

BLAST of Spg036806 vs. NCBI nr
Match: XP_022139908.1 (L-ascorbate oxidase homolog [Momordica charantia])

HSP 1 Score: 1094.3 bits (2829), Expect = 0.0e+00
Identity = 514/542 (94.83%), Postives = 530/542 (97.79%), Query Frame = 0

Query: 80  MRECRVLCSLIFLVLFAVNEVNAENPYRFFTWKVTYGNIYPLGVKQQGILINGQFPGPQI 139
           MRECRVLCSLI LV  AVN  NA+NPYRFFTWKVTYG+IYPLGVKQQGILINGQFPGPQI
Sbjct: 1   MRECRVLCSLIILVFLAVNGANADNPYRFFTWKVTYGDIYPLGVKQQGILINGQFPGPQI 60

Query: 140 DAVTNDNLIINVYNYLKEPFLISWNGLQQRRNSWQDGVYGTTCAIPPLRNFTYVLQAKDQ 199
           DAVTNDNLIINVYNYLKEPFL+SWNGLQQRRNSWQDGVYGTTCAIPP RNFTY++QAKDQ
Sbjct: 61  DAVTNDNLIINVYNYLKEPFLLSWNGLQQRRNSWQDGVYGTTCAIPPFRNFTYIMQAKDQ 120

Query: 200 IGSYFYFPSTAFHKAAGAFGGIRIWSRPGIPVPFAPPAGDFTVLAGDWFKTNHYILRQAL 259
           IGSYFYFPSTAFHKAAGAFGGIRIWSRPGIPVPFAPPAGDFTVLAGDWFKTNHYILRQ L
Sbjct: 121 IGSYFYFPSTAFHKAAGAFGGIRIWSRPGIPVPFAPPAGDFTVLAGDWFKTNHYILRQVL 180

Query: 260 DNGQNLPSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGISTSINFRIQGHVMKLVEVE 319
           D+G+NLPSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVG+S SINFRIQGH+MKLVEVE
Sbjct: 181 DSGRNLPSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGLSASINFRIQGHLMKLVEVE 240

Query: 320 GSHTLQNTYSSLDVHVGQSYSVLVTADQPSKDYYVVVSSRFTSPILTTTAVLHYSNSWQK 379
           GSHTLQNTYSSLD+H+GQSYSVLVTADQP KDYYVVVSSRFTSPILTTTAVLHYSNSWQK
Sbjct: 241 GSHTLQNTYSSLDIHLGQSYSVLVTADQPPKDYYVVVSSRFTSPILTTTAVLHYSNSWQK 300

Query: 380 VSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILANSAPII 439
           +SGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILANSAPII
Sbjct: 301 ISGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILANSAPII 360

Query: 440 NGKQRFAVNSVSFVQADTPLKLADFFKIPGVFSLNSIPTTPTWGNAYLQTSVMGTNFREY 499
           NGKQRFAVNSVSFVQADTPLKLADFFKI GVF+LNSIPTTPTWGNAYLQTSVMG+NFREY
Sbjct: 361 NGKQRFAVNSVSFVQADTPLKLADFFKISGVFNLNSIPTTPTWGNAYLQTSVMGSNFREY 420

Query: 500 IEIVFQNWEDTVQSWHIDGYSFFVVGMDGGQWTPASRARYNLRDTVARCTTQVYPKSWTA 559
           IEIV QNWEDTVQSWHI+GYSFFVVGMDGGQWTPASRARYNLRDTVARCTTQVYPKSWTA
Sbjct: 421 IEIVLQNWEDTVQSWHINGYSFFVVGMDGGQWTPASRARYNLRDTVARCTTQVYPKSWTA 480

Query: 560 IYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSLRDELPIPRNALLCGRASGRHTR 619
           IYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSLRDELP+PRN LLCGRASGRHTR
Sbjct: 481 IYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSLRDELPVPRNXLLCGRASGRHTR 540

Query: 620 PL 622
           PL
Sbjct: 541 PL 542

BLAST of Spg036806 vs. NCBI nr
Match: XP_004153223.1 (L-ascorbate oxidase homolog [Cucumis sativus] >KAE8652663.1 hypothetical protein Csa_013262 [Cucumis sativus])

HSP 1 Score: 1093.6 bits (2827), Expect = 0.0e+00
Identity = 513/542 (94.65%), Postives = 533/542 (98.34%), Query Frame = 0

Query: 80  MRECRVLCSLIFLVLFAVNEVNAENPYRFFTWKVTYGNIYPLGVKQQGILINGQFPGPQI 139
           MRE RVLCSLI LVLFAVNEVNA+NPYRFFTWKVTYG+IYPLGVKQQGILINGQFPGPQI
Sbjct: 1   MREYRVLCSLIILVLFAVNEVNADNPYRFFTWKVTYGDIYPLGVKQQGILINGQFPGPQI 60

Query: 140 DAVTNDNLIINVYNYLKEPFLISWNGLQQRRNSWQDGVYGTTCAIPPLRNFTYVLQAKDQ 199
           DAVTNDNLIINVYNYLKEPFLISWNGLQQRRNSWQDGVYGTTCAIPP+RNFTY LQAKDQ
Sbjct: 61  DAVTNDNLIINVYNYLKEPFLISWNGLQQRRNSWQDGVYGTTCAIPPMRNFTYTLQAKDQ 120

Query: 200 IGSYFYFPSTAFHKAAGAFGGIRIWSRPGIPVPFAPPAGDFTVLAGDWFKTNHYILRQAL 259
           IGSYFYFPSTAFHKAAGAFGGIRIWSRPGIPVPFAPPAGDFTVLAGDW+KTNHYI+RQ L
Sbjct: 121 IGSYFYFPSTAFHKAAGAFGGIRIWSRPGIPVPFAPPAGDFTVLAGDWYKTNHYIMRQVL 180

Query: 260 DNGQNLPSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGISTSINFRIQGHVMKLVEVE 319
           D+G+N+PSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGIS SINFRIQGH+MKLVEVE
Sbjct: 181 DSGKNIPSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGISASINFRIQGHLMKLVEVE 240

Query: 320 GSHTLQNTYSSLDVHVGQSYSVLVTADQPSKDYYVVVSSRFTSPILTTTAVLHYSNSWQK 379
           GSHTLQNTYSSLD+H+GQSYSVLVTA+QP KDYYVVVSSRFTSPILTTTAVLHYSNSWQK
Sbjct: 241 GSHTLQNTYSSLDIHLGQSYSVLVTANQPPKDYYVVVSSRFTSPILTTTAVLHYSNSWQK 300

Query: 380 VSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILANSAPII 439
           VSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTI+LANSAP+I
Sbjct: 301 VSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIVLANSAPVI 360

Query: 440 NGKQRFAVNSVSFVQADTPLKLADFFKIPGVFSLNSIPTTPTWGNAYLQTSVMGTNFREY 499
           NGKQRFAVNSVSFVQADTPLKLAD+FKIPGVF+LN+IPT PTWGNAYLQTSVMG+NFREY
Sbjct: 361 NGKQRFAVNSVSFVQADTPLKLADYFKIPGVFNLNNIPTNPTWGNAYLQTSVMGSNFREY 420

Query: 500 IEIVFQNWEDTVQSWHIDGYSFFVVGMDGGQWTPASRARYNLRDTVARCTTQVYPKSWTA 559
           IEIVFQNWE+TVQSWHI GYSFFVVGMDGGQWTPASR+RYNLRDTVARCTTQVYPKSWTA
Sbjct: 421 IEIVFQNWENTVQSWHISGYSFFVVGMDGGQWTPASRSRYNLRDTVARCTTQVYPKSWTA 480

Query: 560 IYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSLRDELPIPRNALLCGRASGRHTR 619
           IYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTS RDELPIPRNALLCGRASGRHTR
Sbjct: 481 IYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSFRDELPIPRNALLCGRASGRHTR 540

Query: 620 PL 622
           PL
Sbjct: 541 PL 542

BLAST of Spg036806 vs. NCBI nr
Match: XP_023519275.1 (L-ascorbate oxidase homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1093.6 bits (2827), Expect = 0.0e+00
Identity = 516/542 (95.20%), Postives = 532/542 (98.15%), Query Frame = 0

Query: 80  MRECRVLCSLIFLVLFAVNEVNAENPYRFFTWKVTYGNIYPLGVKQQGILINGQFPGPQI 139
           MRE RVLCSLI LVLFAVNEV A+NPYRFFTWKVTYG IYPLGVKQQ ILINGQFPGPQI
Sbjct: 1   MRENRVLCSLIILVLFAVNEVTADNPYRFFTWKVTYGVIYPLGVKQQAILINGQFPGPQI 60

Query: 140 DAVTNDNLIINVYNYLKEPFLISWNGLQQRRNSWQDGVYGTTCAIPPLRNFTYVLQAKDQ 199
           DAVTNDNLIINVYNYLKEPFLISWNGLQQRRNSWQDGVYGTTCAIPPLRNFTYVLQAKDQ
Sbjct: 61  DAVTNDNLIINVYNYLKEPFLISWNGLQQRRNSWQDGVYGTTCAIPPLRNFTYVLQAKDQ 120

Query: 200 IGSYFYFPSTAFHKAAGAFGGIRIWSRPGIPVPFAPPAGDFTVLAGDWFKTNHYILRQAL 259
           IGSYFYFPSTAFHKAAGAFGGIRIWSRPGIPVPFAPPAGDFTVLAGDWFKTNHYILR+ L
Sbjct: 121 IGSYFYFPSTAFHKAAGAFGGIRIWSRPGIPVPFAPPAGDFTVLAGDWFKTNHYILRRVL 180

Query: 260 DNGQNLPSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGISTSINFRIQGHVMKLVEVE 319
           ++G+NLPSPDGLLINGRGWNGYTFNV+PGKTYRFRISNVG+S SINFRIQGHVMKLVEVE
Sbjct: 181 ESGRNLPSPDGLLINGRGWNGYTFNVNPGKTYRFRISNVGLSASINFRIQGHVMKLVEVE 240

Query: 320 GSHTLQNTYSSLDVHVGQSYSVLVTADQPSKDYYVVVSSRFTSPILTTTAVLHYSNSWQK 379
           GSHTLQNTYSSLD+H+GQSYSVLVTADQP KDYYVVVSSRFTSPILTTTAVLHYSNSWQK
Sbjct: 241 GSHTLQNTYSSLDIHLGQSYSVLVTADQPPKDYYVVVSSRFTSPILTTTAVLHYSNSWQK 300

Query: 380 VSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILANSAPII 439
           VSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILANSAP+I
Sbjct: 301 VSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILANSAPVI 360

Query: 440 NGKQRFAVNSVSFVQADTPLKLADFFKIPGVFSLNSIPTTPTWGNAYLQTSVMGTNFREY 499
           NGKQRFAVNSVSFVQADTPLKLAD+FKIPGVF+LNSIPTTPTWGNAYLQTSVMG+NFREY
Sbjct: 361 NGKQRFAVNSVSFVQADTPLKLADYFKIPGVFNLNSIPTTPTWGNAYLQTSVMGSNFREY 420

Query: 500 IEIVFQNWEDTVQSWHIDGYSFFVVGMDGGQWTPASRARYNLRDTVARCTTQVYPKSWTA 559
           +EIVFQNWEDTVQSWHIDGYSFFVVGMDGGQWTPASRARYNLRDTVARCTTQVYP++WTA
Sbjct: 421 VEIVFQNWEDTVQSWHIDGYSFFVVGMDGGQWTPASRARYNLRDTVARCTTQVYPRAWTA 480

Query: 560 IYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSLRDELPIPRNALLCGRASGRHTR 619
           IYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTS RDELPIPRNALLCGRA GRHTR
Sbjct: 481 IYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSFRDELPIPRNALLCGRARGRHTR 540

Query: 620 PL 622
           PL
Sbjct: 541 PL 542

BLAST of Spg036806 vs. NCBI nr
Match: XP_023001247.1 (L-ascorbate oxidase homolog [Cucurbita maxima])

HSP 1 Score: 1093.2 bits (2826), Expect = 0.0e+00
Identity = 516/542 (95.20%), Postives = 531/542 (97.97%), Query Frame = 0

Query: 80  MRECRVLCSLIFLVLFAVNEVNAENPYRFFTWKVTYGNIYPLGVKQQGILINGQFPGPQI 139
           MRE R+LCSLI  VLFAVNEV A+NPYRFFTWKVTYG IYPLGVKQQ ILINGQFPGPQI
Sbjct: 1   MRENRILCSLIIFVLFAVNEVTADNPYRFFTWKVTYGVIYPLGVKQQAILINGQFPGPQI 60

Query: 140 DAVTNDNLIINVYNYLKEPFLISWNGLQQRRNSWQDGVYGTTCAIPPLRNFTYVLQAKDQ 199
           DAVTNDNLIINVYNYLKEPFLISWNGLQQRRNSWQDGVYGTTCAIPPLRNFTYVLQAKDQ
Sbjct: 61  DAVTNDNLIINVYNYLKEPFLISWNGLQQRRNSWQDGVYGTTCAIPPLRNFTYVLQAKDQ 120

Query: 200 IGSYFYFPSTAFHKAAGAFGGIRIWSRPGIPVPFAPPAGDFTVLAGDWFKTNHYILRQAL 259
           IGSYFYFPSTAFHKAAGAFGGIRIWSRPGIPVPFAPPAGDFTVLAGDWFKTNHYILR+ L
Sbjct: 121 IGSYFYFPSTAFHKAAGAFGGIRIWSRPGIPVPFAPPAGDFTVLAGDWFKTNHYILRRVL 180

Query: 260 DNGQNLPSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGISTSINFRIQGHVMKLVEVE 319
           ++G+NLPSPDGLLINGRGWNGYTFNV+PGKTYRFRISNVG+S SINFRIQGHVMKLVEVE
Sbjct: 181 ESGRNLPSPDGLLINGRGWNGYTFNVNPGKTYRFRISNVGLSASINFRIQGHVMKLVEVE 240

Query: 320 GSHTLQNTYSSLDVHVGQSYSVLVTADQPSKDYYVVVSSRFTSPILTTTAVLHYSNSWQK 379
           GSHTLQNTYSSLD+H+GQSYSVLVTADQP KDYYVVVSSRFTSPILTTTAVLHYSNSWQK
Sbjct: 241 GSHTLQNTYSSLDIHLGQSYSVLVTADQPPKDYYVVVSSRFTSPILTTTAVLHYSNSWQK 300

Query: 380 VSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILANSAPII 439
           VSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILANSAPII
Sbjct: 301 VSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILANSAPII 360

Query: 440 NGKQRFAVNSVSFVQADTPLKLADFFKIPGVFSLNSIPTTPTWGNAYLQTSVMGTNFREY 499
           NGKQRFAVNSVSFVQADTPLKLAD+FKIPGVF+LNSIPTTPTWGNAYLQTSVMG+NFREY
Sbjct: 361 NGKQRFAVNSVSFVQADTPLKLADYFKIPGVFNLNSIPTTPTWGNAYLQTSVMGSNFREY 420

Query: 500 IEIVFQNWEDTVQSWHIDGYSFFVVGMDGGQWTPASRARYNLRDTVARCTTQVYPKSWTA 559
           +EIVFQNWEDTVQSWHIDGYSFFVVGMDGGQWTPASRARYNLRDTVARCTTQVYPK+WTA
Sbjct: 421 VEIVFQNWEDTVQSWHIDGYSFFVVGMDGGQWTPASRARYNLRDTVARCTTQVYPKAWTA 480

Query: 560 IYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSLRDELPIPRNALLCGRASGRHTR 619
           IYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTS RDELPIPRNALLCGRA GRHTR
Sbjct: 481 IYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSFRDELPIPRNALLCGRARGRHTR 540

Query: 620 PL 622
           PL
Sbjct: 541 PL 542

BLAST of Spg036806 vs. ExPASy Swiss-Prot
Match: Q00624 (L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1)

HSP 1 Score: 597.8 bits (1540), Expect = 1.4e-169
Identity = 291/541 (53.79%), Postives = 368/541 (68.02%), Query Frame = 0

Query: 80  MRECRVLCSLIFLVLFAVNEVNAENPYRFFTWKVTYGNIYPLGVKQQGILINGQFPGPQI 139
           MR  ++L + ++L   A   V+AE+PY    W VTYG   PLGV QQ ILINGQFPGP I
Sbjct: 1   MRGVKLLAACLYLAAAATVVVHAEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPNI 60

Query: 140 DAVTNDNLIINVYNYLKEPFLISWNGLQQRRNSWQDGVYGTTCAIPPLRNFTYVLQAKDQ 199
           ++ +N+N+IINV+N L EPFL++WNG+Q R+N WQDG  GT C I P  N+TY  Q KDQ
Sbjct: 61  NSTSNNNVIINVFNNLDEPFLLTWNGIQHRKNCWQDGTPGTMCPIMPGTNYTYHFQPKDQ 120

Query: 200 IGSYFYFPSTAFHKAAGAFGGIRIWSRPGIPVPFAPPAGDFTVLAGDWFKTNHYILRQAL 259
           IGSYFY+P+T  H+AAG +GG+R+ SR  IPVP+A P  D+TVL GDW+  +H  L++ L
Sbjct: 121 IGSYFYYPTTGMHRAAGGYGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTQLKKFL 180

Query: 260 DNGQNLPSPDGLLINGR-----GWNGYTFNVDPGKTYRFRISNVGISTSINFRIQGHVMK 319
           D G+ +  PDG++ING+     G +   F + PGKTYR RI NVG+ TSINFRIQ H MK
Sbjct: 181 DGGRTIGRPDGIVINGKSGKGDGSDAPLFTLKPGKTYRVRICNVGVKTSINFRIQNHKMK 240

Query: 320 LVEVEGSHTLQNTYSSLDVHVGQSYSVLVTADQPSKDYYVVVSSRFTSPILTTTAVLHYS 379
           LVE+EGSH LQN Y SLDVHVGQ +  +VTA+Q  KDYY+V SSRF   ++TTT +L Y 
Sbjct: 241 LVEMEGSHVLQNDYDSLDVHVGQCFGTIVTANQEPKDYYMVASSRFLKTVITTTGLLRYE 300

Query: 380 NSWQKVSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILAN 439
                 S  +P GP    AWSL Q RS RWNLTAS  RPNPQGSYHYG I  +RTI L N
Sbjct: 301 GGKGPASSQLPAGP-VGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVN 360

Query: 440 SAPIINGKQRFAVNSVSFVQADTPLKLADFFKIPG-VFSLNSI---PTTPTWGNAYLQTS 499
           +   ++GK RFA+N VS  + +TPLKLA++F I   VF  ++I   PT     N  ++ +
Sbjct: 361 TQGKVDGKLRFALNGVSHTEPETPLKLAEYFGISDKVFKYDTITDDPTPEQIKNIKIEPN 420

Query: 500 VMGTNFREYIEIVFQNWEDTVQSWHIDGYSFFVVGMDGGQWTPASRARYNLRDTVARCTT 559
           V+    R ++E+VF+N E +VQSWH+DGYSFF V ++ G WTP  R  YNL D V+R T 
Sbjct: 421 VLNITHRTFVEVVFENHEKSVQSWHLDGYSFFSVAVEPGTWTPEKRKNYNLLDAVSRHTV 480

Query: 560 QVYPKSWTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSLRDELPIPRNALLC 612
           QVYPK W AI +  DN GMWNVRSE+  R+YLGQQ Y  V +   SLRDE  +P  +L C
Sbjct: 481 QVYPKCWAAILLTFDNCGMWNVRSENTERRYLGQQLYASVLSPEKSLRDEYNMPETSLQC 540

BLAST of Spg036806 vs. ExPASy Swiss-Prot
Match: P29162 (L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 570.5 bits (1469), Expect = 2.4e-161
Identity = 272/541 (50.28%), Postives = 356/541 (65.80%), Query Frame = 0

Query: 88  SLIFLVLFAVNEVNAENPYRFFTWKVTYGNIYPLGVKQQGILINGQFPGPQIDAVTNDNL 147
           + + L+L     V AE+PY +F W VTYG I PLGV QQGILINGQFPGP+I+  +N+N+
Sbjct: 7   TFVALLLCLSVGVIAEDPYLYFNWNVTYGTIAPLGVPQQGILINGQFPGPRINCTSNNNI 66

Query: 148 IINVYNYLKEPFLISWNGLQQRRNSWQDGVYGTTCAIPPLRNFTYVLQAKDQIGSYFYFP 207
           ++NV+N L EPFL +WNG+Q R+NSWQDG  GT C I P +NFTY  Q KDQIGSY YFP
Sbjct: 67  VVNVFNNLDEPFLFTWNGVQHRKNSWQDGTPGTMCPIMPGQNFTYRFQVKDQIGSYSYFP 126

Query: 208 STAFHKAAGAFGGIRIWSRPGIPVPFAPPAGDFTVLAGDWFKTNHYILRQALDNGQNLPS 267
           +TA H+AAG +G + + SR  IPVPF  PA ++ V  GDW+   H  L++ LD G+ +  
Sbjct: 127 TTALHRAAGGYGALNVHSRALIPVPFDNPADEYNVFVGDWYNKGHKTLKKILDGGRTIGR 186

Query: 268 PDGLLINGRG-----WNGYTFNVDPGKTYRFRISNVGISTSINFRIQGHVMKLVEVEGSH 327
           PDG++ING+           F ++ GKTYR+R  N+G+ +S+N R QGH MKLVE+EGSH
Sbjct: 187 PDGIIINGKSAKVGEAKEPLFTMEAGKTYRYRFCNLGMRSSVNIRFQGHPMKLVELEGSH 246

Query: 328 TLQNTYSSLDVHVGQSYSVLVTADQPSKDYYVVVSSRFTSPILTTTAVLHYSNSWQKVSG 387
           T+QN Y SLD+HVGQ  SVLVTADQ  KDYY+VVSSRF    L++ A++ Y+N     S 
Sbjct: 247 TVQNIYDSLDLHVGQCLSVLVTADQEPKDYYLVVSSRFLKQALSSVAIIRYANGKGPASP 306

Query: 388 PVPGGP---TTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILANSAPII 447
            +P  P   T  IAWS+ Q RS RWNLTAS  RPNPQGSYHYG I  +RTI + NS   +
Sbjct: 307 ELPTPPPENTEGIAWSMNQFRSFRWNLTASAARPNPQGSYHYGQINITRTIKIFNSMSQV 366

Query: 448 NGKQRFAVNSVSFVQADTPLKLADFFKIPGV-----FSLNSIPTTPTWGNAYLQTSVMGT 507
            GK R+ +N +S    +TPLKL ++F             +  P  P+     + T+V   
Sbjct: 367 GGKLRYGLNGISHTNGETPLKLVEYFGATNKAFKYDLMADEAPADPS--KLTIATNVKNA 426

Query: 508 NFREYIEIVFQNWEDTVQSWHIDGYSFFVVGMDGGQWTPASRARYNLRDTVARCTTQVYP 567
            +R ++EI+F+N E T++++H+DGYSFF V ++ G+W+P  R  YNL D ++R   QVYP
Sbjct: 427 TYRNFVEIIFENHEKTIRTYHLDGYSFFAVAVEPGRWSPEKRKNYNLVDGLSRNNIQVYP 486

Query: 568 KSWTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSLRDELPIPRNALLCGRAS 616
            SW AI +  DN GMWN+RSE W + YLG+Q Y  V + S SLRDE  IP N  LCG   
Sbjct: 487 NSWAAIMLTFDNAGMWNLRSEMWEKTYLGEQLYFSVLSPSRSLRDEYNIPDNHPLCGIVK 545

BLAST of Spg036806 vs. ExPASy Swiss-Prot
Match: Q9SU40 (Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=1 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 2.2e-151
Identity = 274/550 (49.82%), Postives = 359/550 (65.27%), Query Frame = 0

Query: 86  LCSLIFLVLFA-VNEVNAENPYRFFTWKVTYGNIYPLGVKQQGILINGQFPGPQIDAVTN 145
           L  ++ LV F  ++   A +PY F+ ++V+Y    PLGV QQ I ING+FPGP I+  TN
Sbjct: 3   LFKILLLVFFVNISFCFAADPYSFYNFEVSYITASPLGVPQQVIAINGKFPGPTINVTTN 62

Query: 146 DNLIINVYNYLKEPFLISWNGLQQRRNSWQDGVYGTTCAIPPLRNFTYVLQAKDQIGSYF 205
           +NL++NV N L E  L+ WNG+QQRR SWQDGV GT C IPP  N+TY  Q KDQIGS+F
Sbjct: 63  ENLVVNVRNKLDEGLLLHWNGIQQRRVSWQDGVLGTNCPIPPKWNWTYEFQVKDQIGSFF 122

Query: 206 YFPSTAFHKAAGAFGGIRIWSRPGIPVPFAPPAGDFTVLAGDWFKTNHYILRQALDNGQN 265
           YFPS  F +A+G FG   +  R  IPVPF+ P GD TV  GDW+  NH  LR+ALD+G++
Sbjct: 123 YFPSLHFQRASGGFGSFVVNPRAIIPVPFSTPDGDITVTIGDWYIRNHTALRKALDDGKD 182

Query: 266 LPSPDGLLINGRGWNGY------------TFNVDPGKTYRFRISNVGISTSINFRIQGHV 325
           L  PDG+LING+G   Y            T  V PGKTYR R+SNVGISTS+NFRIQGH 
Sbjct: 183 LGMPDGVLINGKGPYRYNDTLVADGIDFETITVHPGKTYRLRVSNVGISTSLNFRIQGHN 242

Query: 326 MKLVEVEGSHTLQNTYSSLDVHVGQSYSVLVTADQ-PSKDYYVVVSSRFTSPIL----TT 385
           + L E EGS+T+Q  Y+SLD+HVGQSYS LVT DQ  S DYY+V S+R  +  +    T 
Sbjct: 243 LVLAESEGSYTVQQNYTSLDIHVGQSYSFLVTMDQNASSDYYIVASARVVNETIWRRVTG 302

Query: 386 TAVLHYSNSWQKVSGPVPGGPTTEI--AWSLQQARSIRWNLTASGPRPNPQGSYHYGLIK 445
             +L Y+NS  K  G +P GP  E    +S+ QARSIRWN++ASG RPNPQGS+ YG I 
Sbjct: 303 VGILKYTNSKGKAKGQLPPGPQDEFDKTFSMNQARSIRWNVSASGARPNPQGSFKYGSIN 362

Query: 446 TSRTIILANSAPI-INGKQRFAVNSVSFVQADTPLKLADFFKIPGVFSLNSIPTTPTWGN 505
            +   +L N  P+ I+GK+R  +N +SF    TP++LAD  K+  V+ L+  P  P  G 
Sbjct: 363 VTDVYVLRNMPPVTISGKRRTTLNGISFKNPSTPIRLADKLKVKDVYKLD-FPKRPLTGP 422

Query: 506 AYLQTSVMGTNFREYIEIVFQNWEDTVQSWHIDGYSFFVVGMDGGQWTPASRARYNLRDT 565
           A + TS++   +R ++E+V QN +  +QS+H+ GY+FFVVGMD G+WT  SR  YN  D 
Sbjct: 423 AKVATSIINGTYRGFMEVVLQNNDTKMQSYHMSGYAFFVVGMDYGEWTENSRGTYNKWDG 482

Query: 566 VARCTTQVYPKSWTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSLRDELPIP 615
           +AR T QVYP +W+AI ++LDN G WN+R+E+    YLGQ+ Y+RV     + + E   P
Sbjct: 483 IARSTIQVYPGAWSAILISLDNPGAWNLRTENLDSWYLGQETYVRVVNPDENNKTEFGHP 542

BLAST of Spg036806 vs. ExPASy Swiss-Prot
Match: Q8VXX5 (Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=2 SV=1)

HSP 1 Score: 520.8 bits (1340), Expect = 2.2e-146
Identity = 268/551 (48.64%), Postives = 362/551 (65.70%), Query Frame = 0

Query: 83  CRVLCS--LIFLVLFAVNEVNAENPYRFFTWKVTYGNIYPLGVKQQGILINGQFPGPQID 142
           C +L S  L F +L AV+   A +P+  + ++V+Y    PLGV QQ I +NGQFPGP ++
Sbjct: 5   CSLLASFLLCFALLSAVS--FAADPFVSYDFRVSYLTASPLGVPQQVIAVNGQFPGPLLN 64

Query: 143 AVTNDNLIINVYNYLKEPFLISWNGLQQRRNSWQDGVYGTTCAIPPLRNFTYVLQAKDQI 202
           A TN N+++NV+N+L EP L++W G+Q RRNSWQDGV GT C IPP  NFTY  Q KDQI
Sbjct: 65  ATTNYNVVVNVFNHLDEPLLLTWPGIQMRRNSWQDGVLGTNCPIPPRWNFTYQFQVKDQI 124

Query: 203 GSYFYFPSTAFHKAAGAFGGIRIWSRPGIPVPFAPPAGDFTVLAGDWFKTNHYILRQALD 262
           GS+FY PS  F +A+G FG I I +R  IP+PF  P G+   + GDW+  +H  LR+ALD
Sbjct: 125 GSFFYSPSLNFQRASGGFGPIVINNRDIIPIPFPQPDGELIFIIGDWYTQDHKALRRALD 184

Query: 263 NGQNLPSPDGLLINGRGWNGY-----------TFNVDPGKTYRFRISNVGISTSINFRIQ 322
           +G+ L  PDG+LING+G   Y           TF+V+PGKTYR R+ NVGISTS+NFRIQ
Sbjct: 185 SGKELGMPDGVLINGKGPYKYNSSVPDGIDYLTFHVEPGKTYRIRVHNVGISTSLNFRIQ 244

Query: 323 GHVMKLVEVEGSHTLQNTYSSLDVHVGQSYSVLVTADQ-PSKDYYVVVSSRFTSPI---- 382
            H + LVE EG +T Q  ++  DVHVGQSYS LVT DQ  + DYY+V S+RF +      
Sbjct: 245 NHSLLLVETEGHYTSQANFTDFDVHVGQSYSFLVTMDQDATSDYYIVASARFVNETVWQR 304

Query: 383 LTTTAVLHYSNSWQKVSGPVPGGPTTEIA--WS-LQQARSIRWNLTASGPRPNPQGSYHY 442
           +T  A+LHYSNS   VSGP+P  P T+++  WS + Q ++IR N +ASG RPNPQGS+HY
Sbjct: 305 VTGVAILHYSNSKGPVSGPLP-VPKTDVSSPWSAMSQPKTIRQNTSASGARPNPQGSFHY 364

Query: 443 GLIKTSRTIILANSAP-IINGKQRFAVNSVSFVQADTPLKLADFFKIPGVFSLNSIPTTP 502
           G I  + T IL +  P IING  R  +N +SFV   TP++LAD  K+ G + L+  P  P
Sbjct: 365 GQINITNTYILRSLPPTIINGALRATLNGISFVNPSTPVRLADRNKVKGAYKLD-FPDRP 424

Query: 503 TWGNAYLQTSVMGTNFREYIEIVFQNWEDTVQSWHIDGYSFFVVGMDGGQWTPASRARYN 562
                 L  S++   ++ +I++VFQN +  +QS+H+DGYSFFVVGMD G W+   +  YN
Sbjct: 425 FNRPLRLDRSMINATYKGFIQVVFQNNDTKIQSFHVDGYSFFVVGMDFGIWSEDKKGSYN 484

Query: 563 LRDTVARCTTQVYPKSWTAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSLRDE 612
             D ++R T +VYP  WTA+ ++LDNVG+WN+R E+  R YLG++ Y+R+       + E
Sbjct: 485 NWDAISRSTIEVYPGGWTAVLISLDNVGVWNIRVENLDRWYLGEETYMRITNPEEDGKTE 544

BLAST of Spg036806 vs. ExPASy Swiss-Prot
Match: Q9FHN6 (Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=2 SV=1)

HSP 1 Score: 506.1 bits (1302), Expect = 5.5e-142
Identity = 256/530 (48.30%), Postives = 342/530 (64.53%), Query Frame = 0

Query: 102 AENPYRFFTWKVTYGNIYPLGVKQQGILINGQFPGPQIDAVTNDNLIINVYNYLKEPFLI 161
           A +PY  + + ++Y    PLGV QQ I +NG+FPGP I+A TN N+ +NV N+L EP L+
Sbjct: 23  AGDPYVSYDFTLSYITASPLGVPQQVIAVNGKFPGPVINATTNYNVHVNVLNHLDEPLLL 82

Query: 162 SWNGLQQRRNSWQDGVYGTTCAIPPLRNFTYVLQAKDQIGSYFYFPSTAFHKAAGAFGGI 221
           +W G+Q RRNSWQDGV GT C IPP  NFTY  Q KDQIGSYFY PS  F +A+G FG +
Sbjct: 83  TWPGVQMRRNSWQDGVLGTNCPIPPNWNFTYDFQLKDQIGSYFYSPSLNFQRASGGFGAL 142

Query: 222 RIWSRPGIPVPFAPPAGDFTVLAGDWFKTNHYILRQALDNGQNLPSPDGLLINGRGWNGY 281
            I +R  +P+PF  P G+   + GDW+  NH  LR+ LD+G+ L  PDG+LING+G   Y
Sbjct: 143 IINNRDLVPIPFTEPDGEIIFIIGDWYTQNHTALRRILDSGKELGMPDGVLINGKGPFKY 202

Query: 282 -----------TFNVDPGKTYRFRISNVGISTSINFRIQGHVMKLVEVEGSHTLQNTYSS 341
                      T NVDPGKTYR R+ NVGISTS+NFRIQ H + L+E EG +T Q  ++ 
Sbjct: 203 NSSVPDGIEHETVNVDPGKTYRIRVHNVGISTSLNFRIQNHKLLLIETEGRYTSQMNFTD 262

Query: 342 LDVHVGQSYSVLVTADQ-PSKDYYVVVSSRFTSPI----LTTTAVLHYSNSWQKVSGPVP 401
            DVHVGQSYS LVT DQ  + DYY+V S+RF +      +T   +LHYSNS    SGP+P
Sbjct: 263 FDVHVGQSYSFLVTMDQNATSDYYIVASARFVNETVWQRVTGVGILHYSNSKGPASGPLP 322

Query: 402 GGPT-TEIAWS-LQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILANSAPI-INGK 461
              T     WS + Q R+I+ N +ASG RPNPQGS+HYG I  +RT IL +  P  INGK
Sbjct: 323 VSATDVNHPWSAMNQPRAIKQNTSASGARPNPQGSFHYGQINITRTYILRSLPPTKINGK 382

Query: 462 QRFAVNSVSFVQADTPLKLADFFKIPGVFSLNSIPTTPTWGN-AYLQTSVMGTNFREYIE 521
            R  +N +SFV   TP++LAD  K+ G + L+  P  P       L +S++   ++ +I+
Sbjct: 383 LRATLNGISFVNPSTPMRLADDHKVKGDYMLD-FPDRPLDEKLPRLSSSIINATYKGFIQ 442

Query: 522 IVFQNWEDTVQSWHIDGYSFFVVGMDGGQWTPASRARYNLRDTVARCTTQVYPKSWTAIY 581
           ++FQN +  +QS+HIDGY+F+VV MD G W+    + YN  D VAR T +VYP +WTA+ 
Sbjct: 443 VIFQNNDTKIQSFHIDGYAFYVVAMDFGIWSEDRNSSYNNWDAVARSTVEVYPGAWTAVL 502

Query: 582 MALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSLRDELPIPRNALLCG 612
           ++LDNVG+WN+R E+  R YLGQ+ Y+R+     +   E+  P N + CG
Sbjct: 503 ISLDNVGVWNIRVENLDRWYLGQETYMRIINPEENGSTEMDPPENVMYCG 551

BLAST of Spg036806 vs. ExPASy TrEMBL
Match: A0A6J1CGS5 (L-ascorbate oxidase homolog OS=Momordica charantia OX=3673 GN=LOC111010709 PE=3 SV=1)

HSP 1 Score: 1094.3 bits (2829), Expect = 0.0e+00
Identity = 514/542 (94.83%), Postives = 530/542 (97.79%), Query Frame = 0

Query: 80  MRECRVLCSLIFLVLFAVNEVNAENPYRFFTWKVTYGNIYPLGVKQQGILINGQFPGPQI 139
           MRECRVLCSLI LV  AVN  NA+NPYRFFTWKVTYG+IYPLGVKQQGILINGQFPGPQI
Sbjct: 1   MRECRVLCSLIILVFLAVNGANADNPYRFFTWKVTYGDIYPLGVKQQGILINGQFPGPQI 60

Query: 140 DAVTNDNLIINVYNYLKEPFLISWNGLQQRRNSWQDGVYGTTCAIPPLRNFTYVLQAKDQ 199
           DAVTNDNLIINVYNYLKEPFL+SWNGLQQRRNSWQDGVYGTTCAIPP RNFTY++QAKDQ
Sbjct: 61  DAVTNDNLIINVYNYLKEPFLLSWNGLQQRRNSWQDGVYGTTCAIPPFRNFTYIMQAKDQ 120

Query: 200 IGSYFYFPSTAFHKAAGAFGGIRIWSRPGIPVPFAPPAGDFTVLAGDWFKTNHYILRQAL 259
           IGSYFYFPSTAFHKAAGAFGGIRIWSRPGIPVPFAPPAGDFTVLAGDWFKTNHYILRQ L
Sbjct: 121 IGSYFYFPSTAFHKAAGAFGGIRIWSRPGIPVPFAPPAGDFTVLAGDWFKTNHYILRQVL 180

Query: 260 DNGQNLPSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGISTSINFRIQGHVMKLVEVE 319
           D+G+NLPSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVG+S SINFRIQGH+MKLVEVE
Sbjct: 181 DSGRNLPSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGLSASINFRIQGHLMKLVEVE 240

Query: 320 GSHTLQNTYSSLDVHVGQSYSVLVTADQPSKDYYVVVSSRFTSPILTTTAVLHYSNSWQK 379
           GSHTLQNTYSSLD+H+GQSYSVLVTADQP KDYYVVVSSRFTSPILTTTAVLHYSNSWQK
Sbjct: 241 GSHTLQNTYSSLDIHLGQSYSVLVTADQPPKDYYVVVSSRFTSPILTTTAVLHYSNSWQK 300

Query: 380 VSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILANSAPII 439
           +SGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILANSAPII
Sbjct: 301 ISGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILANSAPII 360

Query: 440 NGKQRFAVNSVSFVQADTPLKLADFFKIPGVFSLNSIPTTPTWGNAYLQTSVMGTNFREY 499
           NGKQRFAVNSVSFVQADTPLKLADFFKI GVF+LNSIPTTPTWGNAYLQTSVMG+NFREY
Sbjct: 361 NGKQRFAVNSVSFVQADTPLKLADFFKISGVFNLNSIPTTPTWGNAYLQTSVMGSNFREY 420

Query: 500 IEIVFQNWEDTVQSWHIDGYSFFVVGMDGGQWTPASRARYNLRDTVARCTTQVYPKSWTA 559
           IEIV QNWEDTVQSWHI+GYSFFVVGMDGGQWTPASRARYNLRDTVARCTTQVYPKSWTA
Sbjct: 421 IEIVLQNWEDTVQSWHINGYSFFVVGMDGGQWTPASRARYNLRDTVARCTTQVYPKSWTA 480

Query: 560 IYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSLRDELPIPRNALLCGRASGRHTR 619
           IYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSLRDELP+PRN LLCGRASGRHTR
Sbjct: 481 IYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSLRDELPVPRNXLLCGRASGRHTR 540

Query: 620 PL 622
           PL
Sbjct: 541 PL 542

BLAST of Spg036806 vs. ExPASy TrEMBL
Match: A0A6J1KG06 (L-ascorbate oxidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111495434 PE=3 SV=1)

HSP 1 Score: 1093.2 bits (2826), Expect = 0.0e+00
Identity = 516/542 (95.20%), Postives = 531/542 (97.97%), Query Frame = 0

Query: 80  MRECRVLCSLIFLVLFAVNEVNAENPYRFFTWKVTYGNIYPLGVKQQGILINGQFPGPQI 139
           MRE R+LCSLI  VLFAVNEV A+NPYRFFTWKVTYG IYPLGVKQQ ILINGQFPGPQI
Sbjct: 1   MRENRILCSLIIFVLFAVNEVTADNPYRFFTWKVTYGVIYPLGVKQQAILINGQFPGPQI 60

Query: 140 DAVTNDNLIINVYNYLKEPFLISWNGLQQRRNSWQDGVYGTTCAIPPLRNFTYVLQAKDQ 199
           DAVTNDNLIINVYNYLKEPFLISWNGLQQRRNSWQDGVYGTTCAIPPLRNFTYVLQAKDQ
Sbjct: 61  DAVTNDNLIINVYNYLKEPFLISWNGLQQRRNSWQDGVYGTTCAIPPLRNFTYVLQAKDQ 120

Query: 200 IGSYFYFPSTAFHKAAGAFGGIRIWSRPGIPVPFAPPAGDFTVLAGDWFKTNHYILRQAL 259
           IGSYFYFPSTAFHKAAGAFGGIRIWSRPGIPVPFAPPAGDFTVLAGDWFKTNHYILR+ L
Sbjct: 121 IGSYFYFPSTAFHKAAGAFGGIRIWSRPGIPVPFAPPAGDFTVLAGDWFKTNHYILRRVL 180

Query: 260 DNGQNLPSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGISTSINFRIQGHVMKLVEVE 319
           ++G+NLPSPDGLLINGRGWNGYTFNV+PGKTYRFRISNVG+S SINFRIQGHVMKLVEVE
Sbjct: 181 ESGRNLPSPDGLLINGRGWNGYTFNVNPGKTYRFRISNVGLSASINFRIQGHVMKLVEVE 240

Query: 320 GSHTLQNTYSSLDVHVGQSYSVLVTADQPSKDYYVVVSSRFTSPILTTTAVLHYSNSWQK 379
           GSHTLQNTYSSLD+H+GQSYSVLVTADQP KDYYVVVSSRFTSPILTTTAVLHYSNSWQK
Sbjct: 241 GSHTLQNTYSSLDIHLGQSYSVLVTADQPPKDYYVVVSSRFTSPILTTTAVLHYSNSWQK 300

Query: 380 VSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILANSAPII 439
           VSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILANSAPII
Sbjct: 301 VSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILANSAPII 360

Query: 440 NGKQRFAVNSVSFVQADTPLKLADFFKIPGVFSLNSIPTTPTWGNAYLQTSVMGTNFREY 499
           NGKQRFAVNSVSFVQADTPLKLAD+FKIPGVF+LNSIPTTPTWGNAYLQTSVMG+NFREY
Sbjct: 361 NGKQRFAVNSVSFVQADTPLKLADYFKIPGVFNLNSIPTTPTWGNAYLQTSVMGSNFREY 420

Query: 500 IEIVFQNWEDTVQSWHIDGYSFFVVGMDGGQWTPASRARYNLRDTVARCTTQVYPKSWTA 559
           +EIVFQNWEDTVQSWHIDGYSFFVVGMDGGQWTPASRARYNLRDTVARCTTQVYPK+WTA
Sbjct: 421 VEIVFQNWEDTVQSWHIDGYSFFVVGMDGGQWTPASRARYNLRDTVARCTTQVYPKAWTA 480

Query: 560 IYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSLRDELPIPRNALLCGRASGRHTR 619
           IYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTS RDELPIPRNALLCGRA GRHTR
Sbjct: 481 IYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSFRDELPIPRNALLCGRARGRHTR 540

Query: 620 PL 622
           PL
Sbjct: 541 PL 542

BLAST of Spg036806 vs. ExPASy TrEMBL
Match: A0A6J1E844 (L-ascorbate oxidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111431515 PE=3 SV=1)

HSP 1 Score: 1092.0 bits (2823), Expect = 0.0e+00
Identity = 515/542 (95.02%), Postives = 531/542 (97.97%), Query Frame = 0

Query: 80  MRECRVLCSLIFLVLFAVNEVNAENPYRFFTWKVTYGNIYPLGVKQQGILINGQFPGPQI 139
           MRE RVLCSLI  VLFAVNEV A+NPYRFFTWKVTYG IYPLGVKQQ ILINGQFPGPQI
Sbjct: 1   MRENRVLCSLIIFVLFAVNEVTADNPYRFFTWKVTYGVIYPLGVKQQAILINGQFPGPQI 60

Query: 140 DAVTNDNLIINVYNYLKEPFLISWNGLQQRRNSWQDGVYGTTCAIPPLRNFTYVLQAKDQ 199
           DAVTNDNLIINVYNYLKEPFLISWNGLQQRRNSWQDGVYGTTCAIPPLRNFTYVLQAKDQ
Sbjct: 61  DAVTNDNLIINVYNYLKEPFLISWNGLQQRRNSWQDGVYGTTCAIPPLRNFTYVLQAKDQ 120

Query: 200 IGSYFYFPSTAFHKAAGAFGGIRIWSRPGIPVPFAPPAGDFTVLAGDWFKTNHYILRQAL 259
           IGSYFYFPSTAFHKAAGAFGGIRIWSRPGIPVPFAPPAGDFTVLAGDWFKTNHYILR+ L
Sbjct: 121 IGSYFYFPSTAFHKAAGAFGGIRIWSRPGIPVPFAPPAGDFTVLAGDWFKTNHYILRRVL 180

Query: 260 DNGQNLPSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGISTSINFRIQGHVMKLVEVE 319
           ++G+NLPSPDGLLINGRGWNGYTFNV+PGKTYRFRISNVG+S SINFRIQGHVMKLVEVE
Sbjct: 181 ESGRNLPSPDGLLINGRGWNGYTFNVNPGKTYRFRISNVGLSASINFRIQGHVMKLVEVE 240

Query: 320 GSHTLQNTYSSLDVHVGQSYSVLVTADQPSKDYYVVVSSRFTSPILTTTAVLHYSNSWQK 379
           GSHTLQNTYSSLD+H+GQSYSVLVTADQP KDYYVVVSSRFTSPILTTTAVLHYSNSWQK
Sbjct: 241 GSHTLQNTYSSLDIHLGQSYSVLVTADQPPKDYYVVVSSRFTSPILTTTAVLHYSNSWQK 300

Query: 380 VSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILANSAPII 439
           VSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILANSAP+I
Sbjct: 301 VSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILANSAPVI 360

Query: 440 NGKQRFAVNSVSFVQADTPLKLADFFKIPGVFSLNSIPTTPTWGNAYLQTSVMGTNFREY 499
           NGKQRFAVNSVSFVQADTPLKLAD+FKIPGVF+LNSIPTTPTWGNAYLQTSVMG+NFREY
Sbjct: 361 NGKQRFAVNSVSFVQADTPLKLADYFKIPGVFNLNSIPTTPTWGNAYLQTSVMGSNFREY 420

Query: 500 IEIVFQNWEDTVQSWHIDGYSFFVVGMDGGQWTPASRARYNLRDTVARCTTQVYPKSWTA 559
           +EIVFQNWEDTVQSWHIDGYSFFVVGMDGGQWTPASRARYNLRDTVARCTTQVYP++WTA
Sbjct: 421 VEIVFQNWEDTVQSWHIDGYSFFVVGMDGGQWTPASRARYNLRDTVARCTTQVYPRAWTA 480

Query: 560 IYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSLRDELPIPRNALLCGRASGRHTR 619
           IYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTS RDELPIPRNALLCGRA GRHTR
Sbjct: 481 IYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSFRDELPIPRNALLCGRARGRHTR 540

Query: 620 PL 622
           PL
Sbjct: 541 PL 542

BLAST of Spg036806 vs. ExPASy TrEMBL
Match: E5GC62 (Multicopper oxidase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 1085.9 bits (2807), Expect = 0.0e+00
Identity = 510/542 (94.10%), Postives = 528/542 (97.42%), Query Frame = 0

Query: 80  MRECRVLCSLIFLVLFAVNEVNAENPYRFFTWKVTYGNIYPLGVKQQGILINGQFPGPQI 139
           MRE RVLCSLI LVLFAVNE NA+NPYRFFTWKVTYGNIYPLGV+QQGILINGQFPGPQI
Sbjct: 1   MREYRVLCSLIILVLFAVNEANADNPYRFFTWKVTYGNIYPLGVRQQGILINGQFPGPQI 60

Query: 140 DAVTNDNLIINVYNYLKEPFLISWNGLQQRRNSWQDGVYGTTCAIPPLRNFTYVLQAKDQ 199
           DAVTNDNLIINVYNYLKEPFLISWNGLQQRRNSWQDGVYGTTCAIPP+RNFTY LQAKDQ
Sbjct: 61  DAVTNDNLIINVYNYLKEPFLISWNGLQQRRNSWQDGVYGTTCAIPPMRNFTYTLQAKDQ 120

Query: 200 IGSYFYFPSTAFHKAAGAFGGIRIWSRPGIPVPFAPPAGDFTVLAGDWFKTNHYILRQAL 259
           IGSYFYFPSTAFHKAAGAFGGIRIWSRPGIPVPF  PAGDFTVLAGDW+KTNHYILR+ L
Sbjct: 121 IGSYFYFPSTAFHKAAGAFGGIRIWSRPGIPVPFPSPAGDFTVLAGDWYKTNHYILRRVL 180

Query: 260 DNGQNLPSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGISTSINFRIQGHVMKLVEVE 319
           D+G+NLPSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGIS SINFRIQGH+MKLVEVE
Sbjct: 181 DSGKNLPSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGISASINFRIQGHLMKLVEVE 240

Query: 320 GSHTLQNTYSSLDVHVGQSYSVLVTADQPSKDYYVVVSSRFTSPILTTTAVLHYSNSWQK 379
           GSHTLQNTYSSLD+H+GQSYSVLVTA+QP KDYYVVVSSRFTSPILTTTAVLHYSNSWQK
Sbjct: 241 GSHTLQNTYSSLDIHLGQSYSVLVTANQPPKDYYVVVSSRFTSPILTTTAVLHYSNSWQK 300

Query: 380 VSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILANSAPII 439
           VSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTI+LANSAP+I
Sbjct: 301 VSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIVLANSAPVI 360

Query: 440 NGKQRFAVNSVSFVQADTPLKLADFFKIPGVFSLNSIPTTPTWGNAYLQTSVMGTNFREY 499
           NGKQRFAVN VSFVQADTPLKLAD+FKIPGVF+LNSIPT PTWGNAYLQTSVMG+NFREY
Sbjct: 361 NGKQRFAVNGVSFVQADTPLKLADYFKIPGVFNLNSIPTNPTWGNAYLQTSVMGSNFREY 420

Query: 500 IEIVFQNWEDTVQSWHIDGYSFFVVGMDGGQWTPASRARYNLRDTVARCTTQVYPKSWTA 559
           IEIVFQNWE+TVQSWHI GYSFFVVGMDGGQWTPASR RYNLRDTVARCTTQVYPK+WTA
Sbjct: 421 IEIVFQNWENTVQSWHISGYSFFVVGMDGGQWTPASRGRYNLRDTVARCTTQVYPKAWTA 480

Query: 560 IYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSLRDELPIPRNALLCGRASGRHTR 619
           IYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTS RDELPIPRNALLCGRASGRHTR
Sbjct: 481 IYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSFRDELPIPRNALLCGRASGRHTR 540

Query: 620 PL 622
           PL
Sbjct: 541 PL 542

BLAST of Spg036806 vs. ExPASy TrEMBL
Match: A0A6J1GRW8 (L-ascorbate oxidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111456954 PE=3 SV=1)

HSP 1 Score: 1085.9 bits (2807), Expect = 0.0e+00
Identity = 512/544 (94.12%), Postives = 534/544 (98.16%), Query Frame = 0

Query: 78  IAMRECRVLCSLIFLVLFAVNEVNAENPYRFFTWKVTYGNIYPLGVKQQGILINGQFPGP 137
           +AMR+ RVLC LIF+VLFAV +VNAENPYRFFTWK+TYG+IYPLGVKQQGILINGQFPGP
Sbjct: 1   MAMRKSRVLCFLIFMVLFAVIKVNAENPYRFFTWKITYGDIYPLGVKQQGILINGQFPGP 60

Query: 138 QIDAVTNDNLIINVYNYLKEPFLISWNGLQQRRNSWQDGVYGTTCAIPPLRNFTYVLQAK 197
           QIDAVTNDNLIINVYNYLKEPFLISWNGLQQRRNSWQDGVYGTTCAIPPLRNFTYVLQAK
Sbjct: 61  QIDAVTNDNLIINVYNYLKEPFLISWNGLQQRRNSWQDGVYGTTCAIPPLRNFTYVLQAK 120

Query: 198 DQIGSYFYFPSTAFHKAAGAFGGIRIWSRPGIPVPFAPPAGDFTVLAGDWFKTNHYILRQ 257
           DQIGSYFYFPSTAFHKAAGAFGGIRIWSRPGIPVPFAPPAGDFTVLAGDWFKTNHYILRQ
Sbjct: 121 DQIGSYFYFPSTAFHKAAGAFGGIRIWSRPGIPVPFAPPAGDFTVLAGDWFKTNHYILRQ 180

Query: 258 ALDNGQNLPSPDGLLINGRGWNGYTFNVDPGKTYRFRISNVGISTSINFRIQGHVMKLVE 317
            L++G+NLP+PDGLLINGRGWNG+TFNVD GKTYRFRISNVG+S SINFRIQGH+MKLVE
Sbjct: 181 GLESGRNLPAPDGLLINGRGWNGFTFNVDQGKTYRFRISNVGLSASINFRIQGHLMKLVE 240

Query: 318 VEGSHTLQNTYSSLDVHVGQSYSVLVTADQPSKDYYVVVSSRFTSPILTTTAVLHYSNSW 377
           VEGSHTLQNTYSSLD+H+GQSYSVLVTADQP KDYYVVVSSRFTSPILTTTAVLHYSNSW
Sbjct: 241 VEGSHTLQNTYSSLDIHLGQSYSVLVTADQPPKDYYVVVSSRFTSPILTTTAVLHYSNSW 300

Query: 378 QKVSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILANSAP 437
           +KVSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILANSAP
Sbjct: 301 EKVSGPVPGGPTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILANSAP 360

Query: 438 IINGKQRFAVNSVSFVQADTPLKLADFFKIPGVFSLNSIPTTPTWGNAYLQTSVMGTNFR 497
           +INGKQRFAVNSVSFVQADTPLKLADFFKIPGVF+LNSIPT+PTWGNAYLQTSVM +NFR
Sbjct: 361 VINGKQRFAVNSVSFVQADTPLKLADFFKIPGVFNLNSIPTSPTWGNAYLQTSVMDSNFR 420

Query: 498 EYIEIVFQNWEDTVQSWHIDGYSFFVVGMDGGQWTPASRARYNLRDTVARCTTQVYPKSW 557
           EYIEIVFQNWEDTVQSWHI+GYSFFVVGMDGGQWTPASR  YNLRDTVARCTTQVYPKSW
Sbjct: 421 EYIEIVFQNWEDTVQSWHINGYSFFVVGMDGGQWTPASRDGYNLRDTVARCTTQVYPKSW 480

Query: 558 TAIYMALDNVGMWNVRSESWARQYLGQQFYLRVYTSSTSLRDELPIPRNALLCGRASGRH 617
           TAIYMALDNVG+WNVRSESWARQYLGQQFYLRVYTSSTSLRDELPIPRNALLCGRASGRH
Sbjct: 481 TAIYMALDNVGIWNVRSESWARQYLGQQFYLRVYTSSTSLRDELPIPRNALLCGRASGRH 540

Query: 618 TRPL 622
           TRPL
Sbjct: 541 TRPL 544

BLAST of Spg036806 vs. TAIR 10
Match: AT4G22010.1 (SKU5 similar 4 )

HSP 1 Score: 901.0 bits (2327), Expect = 5.4e-262
Identity = 413/532 (77.63%), Postives = 475/532 (89.29%), Query Frame = 0

Query: 90  IFLVLFAVNEVNAENPYRFFTWKVTYGNIYPLGVKQQGILINGQFPGPQIDAVTNDNLII 149
           I L+L  +N V  +NPYRFFTWK+TYG+IYPLGVKQQGILINGQFPGP IDA+TNDN+II
Sbjct: 9   IVLLLVLINGVLGDNPYRFFTWKITYGDIYPLGVKQQGILINGQFPGPHIDAITNDNIII 68

Query: 150 NVYNYLKEPFLISWNGLQQRRNSWQDGVYGTTCAIPPLRNFTYVLQAKDQIGSYFYFPST 209
           +V+NYLKEPFLISWNG+QQR+NSWQDGV GTTC IPP +NFTYV+Q KDQIGS++YFPS 
Sbjct: 69  SVFNYLKEPFLISWNGVQQRKNSWQDGVVGTTCPIPPGKNFTYVIQVKDQIGSFYYFPSL 128

Query: 210 AFHKAAGAFGGIRIWSRPGIPVPFAPPAGDFTVLAGDWFKTNHYILRQALDNGQNLPSPD 269
           AFHKAAGAFG IR+WSRP IPVPF+PP GDF +LAGDW+KTNHY+LR+ L+ G+NLP+PD
Sbjct: 129 AFHKAAGAFGAIRVWSRPRIPVPFSPPDGDFWLLAGDWYKTNHYVLRRLLEAGRNLPNPD 188

Query: 270 GLLINGRGWNGYTFNVDPGKTYRFRISNVGISTSINFRIQGHVMKLVEVEGSHTLQNTYS 329
           G+LINGRGW G TF V PGKTYRFRISNVG++TS+NFRIQGH MKLVEVEGSHT+QN Y+
Sbjct: 189 GVLINGRGWGGNTFTVQPGKTYRFRISNVGVATSLNFRIQGHTMKLVEVEGSHTVQNIYT 248

Query: 330 SLDVHVGQSYSVLVTADQPSKDYYVVVSSRFTSPILTTTAVLHYSNSWQKVSGPVPGGPT 389
           SLD+H+GQSYSVLVTA+Q  +DYY+V+SSRFT  +LTTT++LHYSNS + VSGPVP GPT
Sbjct: 249 SLDIHLGQSYSVLVTANQAPQDYYIVISSRFTRKVLTTTSILHYSNSRKGVSGPVPNGPT 308

Query: 390 TEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILANSAPIINGKQRFAVNS 449
            +IA SL QAR+IR NLTASGPRPNPQGSYHYGLIK  RTIILANSAP INGKQR+AVN 
Sbjct: 309 LDIASSLYQARTIRRNLTASGPRPNPQGSYHYGLIKPGRTIILANSAPWINGKQRYAVNG 368

Query: 450 VSFVQADTPLKLADFFKIPGVFSLNSIPTTPTWGN-AYLQTSVMGTNFREYIEIVFQNWE 509
            SFV  DTPLKLAD+FKIPGVF+L SIPT+P+ GN  YLQ+SVM  NFRE+IE+VFQNWE
Sbjct: 369 ASFVAPDTPLKLADYFKIPGVFNLGSIPTSPSGGNGGYLQSSVMAANFREFIEVVFQNWE 428

Query: 510 DTVQSWHIDGYSFFVVGMDGGQWTPASRARYNLRDTVARCTTQVYPKSWTAIYMALDNVG 569
           ++VQSWH+ GYSFFVVGMDGGQWTP SRA+YNLRD V+R T QVYP++WTAIY+ALDNVG
Sbjct: 429 NSVQSWHVSGYSFFVVGMDGGQWTPGSRAKYNLRDAVSRSTVQVYPRAWTAIYIALDNVG 488

Query: 570 MWNVRSESWARQYLGQQFYLRVYTSSTSLRDELPIPRNALLCGRASGRHTRP 621
           MWN+RSE+WARQYLGQQFYLRVYTSSTS RDE P P+NAL+CGRA GRHTRP
Sbjct: 489 MWNIRSENWARQYLGQQFYLRVYTSSTSYRDEYPPPKNALMCGRAKGRHTRP 540

BLAST of Spg036806 vs. TAIR 10
Match: AT1G76160.1 (SKU5 similar 5 )

HSP 1 Score: 814.3 bits (2102), Expect = 6.7e-236
Identity = 388/532 (72.93%), Postives = 442/532 (83.08%), Query Frame = 0

Query: 90  IFLVLFAVNEVNAENPYRFFTWKVTYGNIYPLGVKQQGILINGQFPGPQIDAVTNDNLII 149
           +F+ L  +  V AE+PYRFF W +TYG+IYPLGV+QQGILING FPGP I +VTNDNLII
Sbjct: 11  LFIGLSLLFAVTAEDPYRFFEWNITYGDIYPLGVRQQGILINGAFPGPDIHSVTNDNLII 70

Query: 150 NVYNYLKEPFLISWNGLQQRRNSWQDGVYGTTCAIPPLRNFTYVLQAKDQIGSYFYFPST 209
           NVYN L EPFL+SWNG+QQRRNS+ DGVYGTTC IPP +N+TY+LQ KDQIGS++YFPS 
Sbjct: 71  NVYNSLDEPFLLSWNGIQQRRNSFVDGVYGTTCPIPPGKNYTYILQMKDQIGSFYYFPSL 130

Query: 210 AFHKAAGAFGGIRIWSRPGIPVPFAPPAGDFTVLAGDWFKTNHYILRQALDNGQNLPSPD 269
            FHKAAG FGGIRI SRP IPVPF  PAGD TVL GDW+K NH  LR  LDNG+ LP PD
Sbjct: 131 GFHKAAGGFGGIRILSRPRIPVPFPDPAGDTTVLIGDWYKANHTDLRAQLDNGKKLPLPD 190

Query: 270 GLLINGRGWNGYTFNVDPGKTYRFRISNVGISTSINFRIQGHVMKLVEVEGSHTLQNTYS 329
           G+LINGR  +G T NV+ GKTYRFRISNVG+  S+NFRIQ H MK+VEVEG+HTLQ T+S
Sbjct: 191 GILINGRS-SGATLNVEQGKTYRFRISNVGLQDSLNFRIQDHKMKVVEVEGTHTLQTTFS 250

Query: 330 SLDVHVGQSYSVLVTADQPSKDYYVVVSSRFTSPILTTTAVLHYSNSWQKVSGPVPGGPT 389
           SLDVHVGQSYSVLVTADQ  +DYYVVVSSRFTS +LTTT +  YSNS   VSGP+PGGPT
Sbjct: 251 SLDVHVGQSYSVLVTADQTPRDYYVVVSSRFTSNVLTTTGIFRYSNSAGGVSGPIPGGPT 310

Query: 390 TEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILANSAPIINGKQRFAVNS 449
            +I WSL QAR+IR NL+ASGPRPNPQGSYHYG+I T+RTI LA+SA  ++GKQR+AVNS
Sbjct: 311 IQIDWSLNQARAIRTNLSASGPRPNPQGSYHYGMINTTRTIRLASSAGQVDGKQRYAVNS 370

Query: 450 VSFVQADTPLKLADFFKIPGVFSLNSIPTTPTWGNAYLQTSVMGTNFREYIEIVFQNWED 509
           VSF  ADTPLK+AD+FKI GV+   SI   PT G  YL TSVM  ++R ++EI+F+N ED
Sbjct: 371 VSFKPADTPLKIADYFKIDGVYRSGSIQYQPTGGGIYLDTSVMQVDYRTFVEIIFENSED 430

Query: 510 TVQSWHIDGYSFFVVGMDGGQWTPASRARYNLRDTVARCTTQVYPKSWTAIYMALDNVGM 569
            VQSWH+DGYSF+VVGMDGGQW+P SR  YNLRD VARCT QVYP SWTAI +ALDNVGM
Sbjct: 431 IVQSWHLDGYSFWVVGMDGGQWSPDSRNEYNLRDAVARCTVQVYPSSWTAILIALDNVGM 490

Query: 570 WNVRSESWARQYLGQQFYLRVYTSSTSLRDELPIPRNALLCGRASGRHTRPL 622
           WN+RSE WARQYLGQQ YLRVYT STSLRDE PIP+NALLCGRASGR TRPL
Sbjct: 491 WNLRSEFWARQYLGQQLYLRVYTPSTSLRDEYPIPKNALLCGRASGRSTRPL 541

BLAST of Spg036806 vs. TAIR 10
Match: AT1G41830.1 (SKU5-similar 6 )

HSP 1 Score: 810.8 bits (2093), Expect = 7.4e-235
Identity = 388/533 (72.80%), Postives = 440/533 (82.55%), Query Frame = 0

Query: 89  LIFLVLFAVNEVNAENPYRFFTWKVTYGNIYPLGVKQQGILINGQFPGPQIDAVTNDNLI 148
           L+F + F    V AE+PYRFF W VTYG+IYPLGV+QQGILINGQFPGP I +VTNDNLI
Sbjct: 12  LLFCLSFFA-AVTAESPYRFFDWNVTYGDIYPLGVRQQGILINGQFPGPDIHSVTNDNLI 71

Query: 149 INVYNYLKEPFLISWNGLQQRRNSWQDGVYGTTCAIPPLRNFTYVLQAKDQIGSYFYFPS 208
           INV+N L EPFLISWNG+Q RRNS+ DG+YGTTC IPP  N+TY+LQ KDQIGS++YFPS
Sbjct: 72  INVHNSLDEPFLISWNGVQNRRNSYVDGMYGTTCPIPPRSNYTYILQVKDQIGSFYYFPS 131

Query: 209 TAFHKAAGAFGGIRIWSRPGIPVPFAPPAGDFTVLAGDWFKTNHYILRQALDNGQNLPSP 268
            AFHKAAG FGGIRI SRPGIPVPFA PAGD+TVL GDW+K NH  L+  LD G+ LPSP
Sbjct: 132 LAFHKAAGGFGGIRILSRPGIPVPFADPAGDYTVLIGDWYKFNHTDLKSRLDRGRKLPSP 191

Query: 269 DGLLINGRGWNGYTFNVDPGKTYRFRISNVGISTSINFRIQGHVMKLVEVEGSHTLQNTY 328
           DG+LINGR  NG T NV+ GKTYR RISNVG+  S+NFRIQ H MKLVEVEG+HTLQ  +
Sbjct: 192 DGILINGRS-NGATLNVEQGKTYRLRISNVGLQDSLNFRIQNHRMKLVEVEGTHTLQTMF 251

Query: 329 SSLDVHVGQSYSVLVTADQPSKDYYVVVSSRFTSPILTTTAVLHYSNSWQKVSGPVPGGP 388
           SSLDVHVGQSYSVL+TADQ  +DYYVVVSSRFT  I+TTT VL YS S    SGP+PGGP
Sbjct: 252 SSLDVHVGQSYSVLITADQSPRDYYVVVSSRFTDKIITTTGVLRYSGSSTPASGPIPGGP 311

Query: 389 TTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILANSAPIINGKQRFAVN 448
           T ++ WSL QAR+IR NLTASGPRPNPQGSYHYGLI   RTI+  +SA  INGKQR+ VN
Sbjct: 312 TIQVDWSLNQARAIRTNLTASGPRPNPQGSYHYGLIPLIRTIVFGSSAGQINGKQRYGVN 371

Query: 449 SVSFVQADTPLKLADFFKIPGVFSLNSIPTTPTWGNAYLQTSVMGTNFREYIEIVFQNWE 508
           SVSFV ADTPLKLADFFKI GV+ +NSI   PT+G  YL TSV+  ++R +IEIVF+N E
Sbjct: 372 SVSFVPADTPLKLADFFKISGVYKINSISDKPTYGGLYLDTSVLQVDYRTFIEIVFENQE 431

Query: 509 DTVQSWHIDGYSFFVVGMDGGQWTPASRARYNLRDTVARCTTQVYPKSWTAIYMALDNVG 568
           D VQS+H++GYSF+VVGMDGGQW   SR  YNLRD V+R T QVYPKSWTAIY+ALDNVG
Sbjct: 432 DIVQSYHLNGYSFWVVGMDGGQWKTGSRNGYNLRDAVSRSTVQVYPKSWTAIYIALDNVG 491

Query: 569 MWNVRSESWARQYLGQQFYLRVYTSSTSLRDELPIPRNALLCGRASGRHTRPL 622
           MWN+RSE WARQYLGQQ YLRV+TSSTSLRDE PIP+N+ LCGRA GRHTRPL
Sbjct: 492 MWNLRSEFWARQYLGQQLYLRVFTSSTSLRDEYPIPKNSRLCGRARGRHTRPL 542

BLAST of Spg036806 vs. TAIR 10
Match: AT1G21850.1 (SKU5 similar 8 )

HSP 1 Score: 782.7 bits (2020), Expect = 2.2e-226
Identity = 371/531 (69.87%), Postives = 434/531 (81.73%), Query Frame = 0

Query: 88  SLIFLVLFAVNEVNAENPYRFFTWKVTYGNIYPLGVKQQGILINGQFPGPQIDAVTNDNL 147
           ++I  + F ++ V AE+PY+FF W VTYGNI PL V QQGILING+FPGP I AVTNDNL
Sbjct: 10  AMILGLFFLISFVAAEDPYKFFEWHVTYGNISPLKVAQQGILINGKFPGPDIAAVTNDNL 69

Query: 148 IINVYNYLKEPFLISWNGLQQRRNSWQDGVYGTTCAIPPLRNFTYVLQAKDQIGSYFYFP 207
           IINV+N+L EPFLISW+G++  RNS+QDGVYGTTC IPP +N+TY LQ KDQIGS++YFP
Sbjct: 70  IINVFNHLDEPFLISWSGIRNWRNSYQDGVYGTTCPIPPGKNYTYALQVKDQIGSFYYFP 129

Query: 208 STAFHKAAGAFGGIRIWSRPGIPVPFAPPAGDFTVLAGDWFKTNHYILRQALDNGQNLPS 267
           S  FHKAAG FG IRI SRP IPVPF  PAGD+TVL GDW+KTNH  LR  LDNG  LP 
Sbjct: 130 SLGFHKAAGGFGAIRISSRPRIPVPFPAPAGDYTVLIGDWYKTNHKDLRAQLDNGGKLPF 189

Query: 268 PDGLLINGRGWNGYTFNVDPGKTYRFRISNVGISTSINFRIQGHVMKLVEVEGSHTLQNT 327
           PDG+LINGRG +G T N++PGKTYR RISNVG+  S+NFRIQ H MKLVEVEG+HT+Q  
Sbjct: 190 PDGILINGRG-SGATLNIEPGKTYRLRISNVGLQNSLNFRIQNHKMKLVEVEGTHTIQTP 249

Query: 328 YSSLDVHVGQSYSVLVTADQPSKDYYVVVSSRFTSPILTTTAVLHYSNSWQKVSGPVPGG 387
           +SSLDVHVGQSYSVL+TADQP+KDYY+VVSSRFTS IL T  VLHYSNS   VSGP+P  
Sbjct: 250 FSSLDVHVGQSYSVLITADQPAKDYYIVVSSRFTSKILITAGVLHYSNSAGPVSGPIPEA 309

Query: 388 PTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILANSAPIINGKQRFAV 447
           P  ++ WS  QAR+I+ NL ASGPRPNPQG+YHYG IK +RTI LA+SA  INGKQR+AV
Sbjct: 310 P-IQLRWSFDQARAIKTNLAASGPRPNPQGTYHYGKIKVTRTIKLASSAGNINGKQRYAV 369

Query: 448 NSVSFVQADTPLKLADFFKIPGVFSLNSIPTTPTWGNAYLQTSVMGTNFREYIEIVFQNW 507
           NS SF   DTPLKLAD+FKI GV++  SIP  PT G  Y  TSVM T+++ ++EIVF+NW
Sbjct: 370 NSASFYPTDTPLKLADYFKIAGVYNPGSIPDQPTHGAIYPVTSVMQTDYKAFVEIVFENW 429

Query: 508 EDTVQSWHIDGYSFFVVGMDGGQWTPASRARYNLRDTVARCTTQVYPKSWTAIYMALDNV 567
           ED VQ+WH+DGYSFFVVGM+ G+W+ ASR  YNL D V+RCT QVYP+SWTAIY++LDNV
Sbjct: 430 EDIVQTWHLDGYSFFVVGMELGKWSAASRKVYNLNDAVSRCTVQVYPRSWTAIYVSLDNV 489

Query: 568 GMWNVRSESWARQYLGQQFYLRVYTSSTSLRDELPIPRNALLCGRASGRHT 619
           GMWN+RSE W RQYLGQQFY+RVYT STSLRDE  IP+NALLCGRA+G HT
Sbjct: 490 GMWNLRSELWERQYLGQQFYMRVYTPSTSLRDEYLIPKNALLCGRATGHHT 538

BLAST of Spg036806 vs. TAIR 10
Match: AT1G21860.1 (SKU5 similar 7 )

HSP 1 Score: 767.3 bits (1980), Expect = 9.4e-222
Identity = 364/530 (68.68%), Postives = 429/530 (80.94%), Query Frame = 0

Query: 88  SLIFLVLFAVNEVNAENPYRFFTWKVTYGNIYPLGVKQQGILINGQFPGPQIDAVTNDNL 147
           ++I  +LF ++   AE+PYRFF W VTYGNI PLGV QQGILING+FPGP I ++TNDNL
Sbjct: 10  AMITTLLFLISLAFAEDPYRFFEWHVTYGNISPLGVAQQGILINGKFPGPDIISITNDNL 69

Query: 148 IINVYNYLKEPFLISWNGLQQRRNSWQDGVYGTTCAIPPLRNFTYVLQAKDQIGSYFYFP 207
           IINV+N+L EPFL+SWNG++  +NS+QDGVYGT C IPP +N+TY LQ KDQIGS++YFP
Sbjct: 70  IINVFNHLDEPFLLSWNGIRNWKNSFQDGVYGTMCPIPPGKNYTYALQVKDQIGSFYYFP 129

Query: 208 STAFHKAAGAFGGIRIWSRPGIPVPFAPPAGDFTVLAGDWFKTNHYILRQALDNGQNLPS 267
           S  FHKAAG FGGIRI SR  IPVPF  PA D+T+L GDW+KTNH  L+  LDNG  LP 
Sbjct: 130 SLGFHKAAGGFGGIRISSRALIPVPFPTPADDYTLLVGDWYKTNHKDLKAQLDNGGKLPL 189

Query: 268 PDGLLINGRGWNGYTFNVDPGKTYRFRISNVGISTSINFRIQGHVMKLVEVEGSHTLQNT 327
           PDG+LINGR  +G T N++PGKTYR RISNVG+  S+NFRIQ H MKLVEVEG +T+QN 
Sbjct: 190 PDGILINGRS-SGATLNIEPGKTYRLRISNVGLQNSLNFRIQNHTMKLVEVEGRYTIQNL 249

Query: 328 YSSLDVHVGQSYSVLVTADQPSKDYYVVVSSRFTSPILTTTAVLHYSNSWQKVSGPVPGG 387
           +SSLDVHVGQSYSVL+TADQP+KDYYVVVSSRFTS ILTTT VLHYSNS   VSGP+P G
Sbjct: 250 FSSLDVHVGQSYSVLITADQPAKDYYVVVSSRFTSKILTTTGVLHYSNSVAPVSGPIPDG 309

Query: 388 PTTEIAWSLQQARSIRWNLTASGPRPNPQGSYHYGLIKTSRTIILANSAPIINGKQRFAV 447
           P  +++WS  QAR+IR NLTASGPRPNPQGSY YG+I  +RTI LAN+   I GKQR+AV
Sbjct: 310 P-IKLSWSFNQARAIRTNLTASGPRPNPQGSYRYGVINITRTIRLANNLGHIEGKQRYAV 369

Query: 448 NSVSFVQADTPLKLADFFKIPGVFSLNSIPTTPTWGNAYLQTSVMGTNFREYIEIVFQNW 507
           NS SF  ADTPLKL D+FKI GV+   SI   PT G  +  TSVM  +FR ++E++F+N 
Sbjct: 370 NSASFYPADTPLKLVDYFKIDGVYKPGSISDQPTNGAIFPTTSVMQADFRAFVEVIFENS 429

Query: 508 EDTVQSWHIDGYSFFVVGMDGGQWTPASRARYNLRDTVARCTTQVYPKSWTAIYMALDNV 567
           ED VQSWH+DGYSF+VVGM+ G+W+PASR  YNL D + RCT QVYP+SWTAIY+ALDNV
Sbjct: 430 EDIVQSWHLDGYSFYVVGMELGKWSPASRKVYNLNDAILRCTIQVYPRSWTAIYIALDNV 489

Query: 568 GMWNVRSESWARQYLGQQFYLRVYTSSTSLRDELPIPRNALLCGRASGRH 618
           GMWN+RSE W RQYLGQQFY+RVYT+STSLRDE  IP+NALLCGRAS  H
Sbjct: 490 GMWNMRSEIWERQYLGQQFYMRVYTTSTSLRDEYLIPKNALLCGRASSSH 537