Spg036573 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg036573
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionheat stress transcription factor A-4c-like
Locationscaffold5: 40790648 .. 40794856 (+)
RNA-Seq ExpressionSpg036573
SyntenySpg036573
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTCATGTTGCCACCCATTACAATTATCCTAAATTTAACTCACTTGGGAACGAGGAAAAACAATTTTACCCAAAAAAAAAAAAAGGGAAATTATTTTTTTTTCCAAGAAAAAAAGTTTTTTTTGACAAAAAAAAAAAAAAAAAAAAAAACAAAGAGGGAGAAATCCGTAATTTGAAACGTTGCCAATGTTTATCTTACACGTCCACCCCTTCAATTTCCCACATTTTAATAATTGGACGCTTAATAATGGTTGGCTTATCTTCCAAGTTCGTATCTTTATTTTCTTTTCCACCATTTTAGTTACTGCTTCTTGTTTTTTTTTTTCAAGGCCGGGTTTGGTCATCGTCAAATAACGATTTCTTCATTTTCCATACATAATTTCTGCTAATCGAGTGAAGGAAAAGAACTGCTTGTTTTGTTGCAACTACAGGTGCCTGGTCTACGTTATCCACAGTATGTCGAGCTTTGATTTTTAATCCTCTCTTTTTGCCTCTTTTTGTTTGTTGAATTCAAGGGGATTTTTTAATCGTCTCTTTTTAATCCCTTTTTATAATTTGATGATTTGGTTTGAATTGATTAGATTAGATTGCGCAAGTATTTTATTAGTTCAATTCAAACCGTTAACTTCAATTCTGTAACTTTCTTTTCTTTTGTCTTCTTTAGGCTAAGTTGAGTAAGTTCTACTTGACTTGACTGGAGCTGGGTCGATGCAAATTTCGTATTCTCTTTCAAAATCCCTTATGGGAACTCGAGATTCTGAATTGGGGTTCCCAGGAAATCAAGCTTCGATGCGAACCTCTGAGATTTTGCGATGATTTTGTAGTTTTACGAAGACCCAACTTGGGTTTTTCTCGCTTTTGAGGTTTGAAGTTCTTTTCTAGTGGGTGGATTATCATTGTTGTTCTCGAAGTTTGAGGTTGTGAATTTCTTAGATTTCTATTGATATTTTTTCAAGGATTTTGATACTTGGTCTTCTGTTTGCAATTTGGTGGATCGAGGGTTGTTAATGTATAGCTTCTGGATTTTGAATTTGGGGGTTTTTGTTTTGGGGATTGGAAGGGTTAGGATTTAGAATGGATGAAGTTCAGGGAGGCGGCTTGACTTCGCTGCCTCCGTTTTTAGTCAAAACATACGATATGGTCGATGATCCTTCAACCAATTCGATTGTTTCATGGAGTTCTAGTGATAAAAGCTTTGTTGTTTGGAATCCGCCCGAGTTCTCATCAGTTTTGTTGCCAAAGTTCTTTAAGCATAGCAACTTCTCGAGCTTCATCAGGCAGCTCAATACTTATGTAAGATGATAAGTTTCTTATTAATTTGCTTTGTGCTGTCTGTTTCGTTACTATAGTTGTGGGTGTGTTCTTGTTTTTGTTTTCTGAATCGTTTGTTGATCACTATAGGGGTTCAGGAAGGTGGATCCTGAACAATGGGAATTTGCCAATGAGGATTTTGTCAAAGGTCAGCCACACCTGATGAGGAACATCCACAGGAGAAAACCAGTTCATAGTCATTCTTTACAGAACCTCCATGGACAAGGAATATCTCCTCCGCTTACTGAAGTGGAAAGAAAGAGTTTAAAGGATGACATTGAAAGGCTTAAACAGGATAAAGAGCAGCATCTTCTGGAGTTACGAAAAAACGAACAAGAGTATCAAGAAGTTAATTTGCAAATGCAGAATTTGAAAGATCGATTTCAACTCGTGCAACAAGAGATGCAATCATTTAGCAGTTGGATGGCTCGCCTTTTGAAGAAACCTGGACTTCGCTTGGATCCTCTGCCACAATTGGAAACTCTTGAGAGAAAGAGGAGGTTACCTAGAGTTTCTTACAACAATAGTGAAGACAACGTTGAGGATAATCAGATGGGGACGACTCAAACCATTGGTAGAGAAAACATGGATTGTAGTTCTGATCCAATTTTGAAAACAGAGCAGTTTGAACTTGTTGAGACATCTCTGACCTTCTGGGAAGGCATTATCCATAGTTACGGTCAAAGAGTTAGTCCACTTGATTCAAGCTCAAACCTGGAGTTGGATGGATCTGTAAGTCATGCCAGTAGCCCTGCTATATCTTGCAGACAAGTTAGTGAGGAGTTTCGGTGTAAATCACCTGGAATTGACATGAATTTGGAGCCCATGGCAACCGTTGCTCCTGAATCTGTAGCCTCGAAAGATCAAGCAGCTGGAGTCAATGCTCCAGTACCAACTGGAGTCAATGATGTTTTCTGGCAACAATTCTTGACGGAGAATCCTGGTTCATCTGACCCGCAAGAAGTTCAATCAGCCAGAAAAGATTCTGATGTCATAAACGAAGAAAACAGGCAGAATGATCACGGAAAATTTTGGTGGAATGTGAGGAATGTAAATAACGTTGTAGAACAGATAGGGCACCTTACTCCAGCAGAGAAATTTTGATAGTAGGTTGATTGTTTTTCTGAATTATTGATTCCCCTTAGCTCACATGTAACATATTTTATCATGAATGGAACATTTGGGCTCTCACCCAACCCATATCTTTTTCAGATGATCTCATTGATTTTAATTCCCTCATAATGCTTGACTTGTTTAGAGAATTACTGGCTGTACCTGGGAACGAATACTTGTTTTGACTGCTTTGTTATTTAATAGGGAATGTACATTAGAGTTGGATCAGTGCGTAGTATGCAAGTGGTTATTTTTTAGGAATTTGAATCCCGTGGAACTATCTTTATATTGCTTTTTCATTATTCAAGTTTGCTAAATTCGTTACTATAGTATTCTAACGTTCTCTCTTGTTTCAATCTCTTCTTTTCATTGGGATCATTTAGGTCAGGATGCATTCTGCATTGATCAGATAGGTATCTTTGCTGGCTTCAGCATCTCCTCCTAAGATCCTATGTTGCCTCATACTGGTGTCCTTTTAATAGAAATCTGTACATTGTCTATTCTGGTTCAATCTTCACTTGCAAAATATTATTAGAATCTTCACAGCATCGTTGGTCCGAGCATAGTTTAGTAGATAAGACACATATTACCTTCCGAAGGTCAATGGTTTGATTCCCCACCCTCATATTTGCTCAACTCCAAAAAGAAAAAAAAAATCTTCAGACTATCACTGCTTTTGGAGTCTCTAGCTCTTGAGAGACGGGTGAAGGGTCTCTTACCTTTTTAAGGATGTAGAAACCAAACATGCTTCTACTCTGATGATACAGCAACACGGTTAGCTTAAAAGTTAGCCACTGTTCTGTCTGCACACAAAAGTTAACTCTTCAAAGTTGGCATTACTACTGAATTGACTGTCTTCTTTTTCAATGATTTCATATGGTGCTTCCTATTGATCATATATACTCCGGGATCATGAATAGGGAAAGTAATTTAGGGAGAGTGGTTCAGCCAAAAGTTCACTCCCATTTGTTCTCTGTTTCCTATAGTCTATCAATATTGATGGTGTTTATCTTTTGTTATGGAGATATCAATGGTTTATCTTTTGTTATGGAGATATCAATGAAGTATCATGAGACTCTATAACATGACAAGAAGGAGTTGATATTCTTGTTTAATATTTCATCATTTTAGTTTTGTTATTAAACAGTATCTATCTAGATTGTAGAGTTGTGGCTCCTTCCTTCTGAGTACGTCATATACCCGCCAGCACCCATCTTTAGAGAAGGTTCTATTGGTTCAAGTCTTTAACTAGTCTAAGCAAGGATGTATCCGCATATCTGCTAGTTTAATGGAAAGTGTCTCTTATCTGAACAAACTCATCTGACATCTGAATAAAAAATATCCCTTTTTGTTCAATGTAAAGTGATATCTCATATCTGATGGCTCCATCTGGAATTTTCTGGACTGTAGGGAGGTATTTTTCTTCTTTGAGGTATGTTCATGATTTTAAGCATCACTTCCTGATTCTTTTGGCTATTGTAGATAACTTTTGAACATGTTGAAAGCGCGATTTCGAACTACAATAAGTTCATGAAGATGAAGGACAACCTTACTGGATCTGCAACTGCCACTCTGTGAAATTGACATGGAATCTGCTAAAGGAAACATCCAGATCTCACCTTTTGATTGCTACTTGTCTTTGCATATTGGCTTACACGACCGAATTGTTAATCCAAACCACTGTGGCTCTTTGCTAATCCTTACCGTGGCGGTTACTATCAGAAAGAACATGGTCGTTATGGGAATCAAAGCCAATGTTAGGACCTCTGATGCTGGGAAGTTGTGA

mRNA sequence

ATGGATGAAGTTCAGGGAGGCGGCTTGACTTCGCTGCCTCCGTTTTTAGTCAAAACATACGATATGGTCGATGATCCTTCAACCAATTCGATTGTTTCATGGAGTTCTAGTGATAAAAGCTTTGTTGTTTGGAATCCGCCCGAGTTCTCATCAGTTTTGTTGCCAAAGTTCTTTAAGCATAGCAACTTCTCGAGCTTCATCAGGCAGCTCAATACTTATGGGTTCAGGAAGGTGGATCCTGAACAATGGGAATTTGCCAATGAGGATTTTGTCAAAGGTCAGCCACACCTGATGAGGAACATCCACAGGAGAAAACCAGTTCATAGTCATTCTTTACAGAACCTCCATGGACAAGGAATATCTCCTCCGCTTACTGAAGTGGAAAGAAAGAGTTTAAAGGATGACATTGAAAGGCTTAAACAGGATAAAGAGCAGCATCTTCTGGAGTTACGAAAAAACGAACAAGAGTATCAAGAAGTTAATTTGCAAATGCAGAATTTGAAAGATCGATTTCAACTCGTGCAACAAGAGATGCAATCATTTAGCAGTTGGATGGCTCGCCTTTTGAAGAAACCTGGACTTCGCTTGGATCCTCTGCCACAATTGGAAACTCTTGAGAGAAAGAGGAGGTTACCTAGAGTTTCTTACAACAATAGTGAAGACAACGTTGAGGATAATCAGATGGGGACGACTCAAACCATTGGTAGAGAAAACATGGATTGTAGTTCTGATCCAATTTTGAAAACAGAGCAGTTTGAACTTGTTGAGACATCTCTGACCTTCTGGGAAGGCATTATCCATAGTTACGGTCAAAGAGTTAGTCCACTTGATTCAAGCTCAAACCTGGAGTTGGATGGATCTGTAAGTCATGCCAGTAGCCCTGCTATATCTTGCAGACAAGTTAGTGAGGAGTTTCGGTGTAAATCACCTGGAATTGACATGAATTTGGAGCCCATGGCAACCGTTGCTCCTGAATCTGTAGCCTCGAAAGATCAAGCAGCTGGAGTCAATGCTCCAGTACCAACTGGAGTCAATGATGTTTTCTGGCAACAATTCTTGACGGAGAATCCTGGTTCATCTGACCCGCAAGAAGTTCAATCAGCCAGAAAAGATTCTGATGTCATAAACGAAGAAAACAGGCAGAATGATCACGGAAAATTTTGGTGGAATGTGAGGAATATAACTTTTGAACATGTTGAAAGCGCGATTTCGAACTACAATAAGTTCATGAAGATGAAGGACAACCTTACTGGATCTGCAACTGCCACTCTAAAGAACATGGTCGTTATGGGAATCAAAGCCAATGTTAGGACCTCTGATGCTGGGAAGTTGTGA

Coding sequence (CDS)

ATGGATGAAGTTCAGGGAGGCGGCTTGACTTCGCTGCCTCCGTTTTTAGTCAAAACATACGATATGGTCGATGATCCTTCAACCAATTCGATTGTTTCATGGAGTTCTAGTGATAAAAGCTTTGTTGTTTGGAATCCGCCCGAGTTCTCATCAGTTTTGTTGCCAAAGTTCTTTAAGCATAGCAACTTCTCGAGCTTCATCAGGCAGCTCAATACTTATGGGTTCAGGAAGGTGGATCCTGAACAATGGGAATTTGCCAATGAGGATTTTGTCAAAGGTCAGCCACACCTGATGAGGAACATCCACAGGAGAAAACCAGTTCATAGTCATTCTTTACAGAACCTCCATGGACAAGGAATATCTCCTCCGCTTACTGAAGTGGAAAGAAAGAGTTTAAAGGATGACATTGAAAGGCTTAAACAGGATAAAGAGCAGCATCTTCTGGAGTTACGAAAAAACGAACAAGAGTATCAAGAAGTTAATTTGCAAATGCAGAATTTGAAAGATCGATTTCAACTCGTGCAACAAGAGATGCAATCATTTAGCAGTTGGATGGCTCGCCTTTTGAAGAAACCTGGACTTCGCTTGGATCCTCTGCCACAATTGGAAACTCTTGAGAGAAAGAGGAGGTTACCTAGAGTTTCTTACAACAATAGTGAAGACAACGTTGAGGATAATCAGATGGGGACGACTCAAACCATTGGTAGAGAAAACATGGATTGTAGTTCTGATCCAATTTTGAAAACAGAGCAGTTTGAACTTGTTGAGACATCTCTGACCTTCTGGGAAGGCATTATCCATAGTTACGGTCAAAGAGTTAGTCCACTTGATTCAAGCTCAAACCTGGAGTTGGATGGATCTGTAAGTCATGCCAGTAGCCCTGCTATATCTTGCAGACAAGTTAGTGAGGAGTTTCGGTGTAAATCACCTGGAATTGACATGAATTTGGAGCCCATGGCAACCGTTGCTCCTGAATCTGTAGCCTCGAAAGATCAAGCAGCTGGAGTCAATGCTCCAGTACCAACTGGAGTCAATGATGTTTTCTGGCAACAATTCTTGACGGAGAATCCTGGTTCATCTGACCCGCAAGAAGTTCAATCAGCCAGAAAAGATTCTGATGTCATAAACGAAGAAAACAGGCAGAATGATCACGGAAAATTTTGGTGGAATGTGAGGAATATAACTTTTGAACATGTTGAAAGCGCGATTTCGAACTACAATAAGTTCATGAAGATGAAGGACAACCTTACTGGATCTGCAACTGCCACTCTAAAGAACATGGTCGTTATGGGAATCAAAGCCAATGTTAGGACCTCTGATGCTGGGAAGTTGTGA

Protein sequence

MDEVQGGGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWNPPEFSSVLLPKFFKHSNFSSFIRQLNTYGFRKVDPEQWEFANEDFVKGQPHLMRNIHRRKPVHSHSLQNLHGQGISPPLTEVERKSLKDDIERLKQDKEQHLLELRKNEQEYQEVNLQMQNLKDRFQLVQQEMQSFSSWMARLLKKPGLRLDPLPQLETLERKRRLPRVSYNNSEDNVEDNQMGTTQTIGRENMDCSSDPILKTEQFELVETSLTFWEGIIHSYGQRVSPLDSSSNLELDGSVSHASSPAISCRQVSEEFRCKSPGIDMNLEPMATVAPESVASKDQAAGVNAPVPTGVNDVFWQQFLTENPGSSDPQEVQSARKDSDVINEENRQNDHGKFWWNVRNITFEHVESAISNYNKFMKMKDNLTGSATATLKNMVVMGIKANVRTSDAGKL
Homology
BLAST of Spg036573 vs. NCBI nr
Match: KAG6583940.1 (Heat stress transcription factor A-4a, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 684.9 bits (1766), Expect = 4.7e-193
Identity = 357/463 (77.11%), Postives = 380/463 (82.07%), Query Frame = 0

Query: 1   MDEVQGGGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWNPPEFSSVLLPKFFKH 60
           MDE QG GLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVW P EFSSVLLPKFFKH
Sbjct: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60

Query: 61  SNFSSFIRQLNTYGFRKVDPEQWEFANEDFVKGQPHLMRNIHRRKPVHSHSLQNLHGQGI 120
           SNFSSFIRQLNTYGF+KVDP+QWEFAN+DFV+G+ HLM+NIHRRKPVHSHSLQN HGQG+
Sbjct: 61  SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV 120

Query: 121 SPPLTEVERKSLKDDIERLKQDKEQHLLELRKNEQEYQEVNLQMQNLKDRFQLVQQEMQS 180
           SPPLTEVERKS +D+IE LK+DKEQ LLELRK+EQEYQ V LQMQNLKDRFQ VQQ MQ 
Sbjct: 121 SPPLTEVERKSFEDNIETLKRDKEQLLLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQL 180

Query: 181 FSSWMARLLKKPGLRLDPLPQLETLERKRRLPRVSYNNSEDNVEDNQMGTTQTIGRENMD 240
           F S +AR L KPGLRLD LPQLET +RKRRLPRVSYNNSEDN+EDNQMGTTQTI REN D
Sbjct: 181 FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENRD 240

Query: 241 CSSDPILKTEQFELVETSLTFWEGIIHSYGQRVSPLDSSSNLELDGSVSHASSPAISCRQ 300
           CS DPILK EQFELVETSLTFWEGIIHSYGQ +SPLDSSSNLEL GSVSHASSPA++CRQ
Sbjct: 241 CSFDPILKEEQFELVETSLTFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300

Query: 301 VSEEFRCKSPGIDMNLEPMATVAPESVASKDQAAGVNAPVPTGVNDVFWQQFLTENPGSS 360
           VSEE RCKSPGIDMNLEPMATVAPESVASKDQAAGV APVPTGVNDVFWQQFLTENPGSS
Sbjct: 301 VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS 360

Query: 361 DPQEVQSARKDSDVINEENRQNDHGKFWWNVRNITF------------------------ 420
           DPQEVQSARKDSDVI EENR +D G FWWN R+  F                        
Sbjct: 361 DPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSRRFYLFEDLNDDLLRTSNRLQNGGRRL 420

Query: 421 ------------------EHVESAISNYNKFMKMKDNLTGSAT 422
                              HVE AI NYNK MK+KD+ T ++T
Sbjct: 421 LWPLRIYTGFRACLFHVTVHVERAILNYNKLMKVKDSPTAAST 463

BLAST of Spg036573 vs. NCBI nr
Match: XP_022927503.1 (heat stress transcription factor A-4c-like [Cucurbita moschata] >XP_022927504.1 heat stress transcription factor A-4c-like [Cucurbita moschata] >KAG7019556.1 Heat stress transcription factor A-4a [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 677.2 bits (1746), Expect = 9.8e-191
Identity = 341/394 (86.55%), Postives = 361/394 (91.62%), Query Frame = 0

Query: 1   MDEVQGGGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWNPPEFSSVLLPKFFKH 60
           MDE QG GLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVW P EFSSVLLPKFFKH
Sbjct: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60

Query: 61  SNFSSFIRQLNTYGFRKVDPEQWEFANEDFVKGQPHLMRNIHRRKPVHSHSLQNLHGQGI 120
           SNFSSFIRQLNTYGF+KVDP+QWEFAN+DFV+G+ HLM+NIHRRKPVHSHSLQN HGQG+
Sbjct: 61  SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV 120

Query: 121 SPPLTEVERKSLKDDIERLKQDKEQHLLELRKNEQEYQEVNLQMQNLKDRFQLVQQEMQS 180
           SPPLTEVERKS +D+IE LK+DKEQ LLELRK+EQEYQ V LQMQNLKDRFQ VQQ MQ 
Sbjct: 121 SPPLTEVERKSFEDNIETLKRDKEQLLLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQL 180

Query: 181 FSSWMARLLKKPGLRLDPLPQLETLERKRRLPRVSYNNSEDNVEDNQMGTTQTIGRENMD 240
           F S +AR L KPGLRLD LPQLET +RKRRLPRVSYNNSEDN+EDNQMGTTQTI REN D
Sbjct: 181 FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENRD 240

Query: 241 CSSDPILKTEQFELVETSLTFWEGIIHSYGQRVSPLDSSSNLELDGSVSHASSPAISCRQ 300
           CS DPILK EQFELVETSLTFWEGIIHSYGQ +SPLDSSSNLEL GSVSHASSPA++CRQ
Sbjct: 241 CSFDPILKEEQFELVETSLTFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300

Query: 301 VSEEFRCKSPGIDMNLEPMATVAPESVASKDQAAGVNAPVPTGVNDVFWQQFLTENPGSS 360
           VSEE RCKSPGIDMNLEPMATVAPESVASKDQAAGV APVPTGVNDVFWQQFLTENPGSS
Sbjct: 301 VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS 360

Query: 361 DPQEVQSARKDSDVINEENRQNDHGKFWWNVRNI 395
           DPQEVQSARKDSDVI EENR +D G FWWN R++
Sbjct: 361 DPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSV 394

BLAST of Spg036573 vs. NCBI nr
Match: XP_011657567.1 (heat stress transcription factor A-4c [Cucumis sativus] >XP_031736076.1 heat stress transcription factor A-4c [Cucumis sativus] >KGN65603.1 hypothetical protein Csa_019674 [Cucumis sativus])

HSP 1 Score: 676.0 bits (1743), Expect = 2.2e-190
Identity = 343/394 (87.06%), Postives = 364/394 (92.39%), Query Frame = 0

Query: 1   MDEVQGGGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWNPPEFSSVLLPKFFKH 60
           MDE QGGGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWNP EFSSVLLPKFFKH
Sbjct: 1   MDEAQGGGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWNPLEFSSVLLPKFFKH 60

Query: 61  SNFSSFIRQLNTYGFRKVDPEQWEFANEDFVKGQPHLMRNIHRRKPVHSHSLQNLHGQGI 120
           SNFSSFIRQLNTYGFRKVDPEQWEFANEDFV+G+PHLM+NIHRRKP+HSHSLQNLHGQGI
Sbjct: 61  SNFSSFIRQLNTYGFRKVDPEQWEFANEDFVRGKPHLMKNIHRRKPIHSHSLQNLHGQGI 120

Query: 121 SPPLTEVERKSLKDDIERLKQDKEQHLLELRKNEQEYQEVNLQMQNLKDRFQLVQQEMQS 180
           S PLTEVER S KDDIERLK DKEQ LLEL+K EQEYQ V LQ+QNLKDRFQ VQQEMQ 
Sbjct: 121 S-PLTEVERNSFKDDIERLKLDKEQLLLELQKYEQEYQGVGLQIQNLKDRFQRVQQEMQL 180

Query: 181 FSSWMARLLKKPGLRLDPLPQLETLERKRRLPRVSYNNSEDNVEDNQMGTTQTIGRENMD 240
           F S MARLL+KPGL LD LPQLET ERKRRLPRVSYN SED++EDN +GTTQTIGR++M 
Sbjct: 181 FISLMARLLQKPGLHLDLLPQLETPERKRRLPRVSYNISEDSLEDNHLGTTQTIGRDDMG 240

Query: 241 CSSDPILKTEQFELVETSLTFWEGIIHSYGQRVSPLDSSSNLELDGSVSHASSPAISCRQ 300
           CS DPIL+ EQ EL+ETSLTFWEGIIHSY + VSPLDSSSNLEL GSVSHASSPAISCR 
Sbjct: 241 CSFDPILEKEQLELLETSLTFWEGIIHSYDETVSPLDSSSNLELVGSVSHASSPAISCRL 300

Query: 301 VSEEFRCKSPGIDMNLEPMATVAPESVASKDQAAGVNAPVPTGVNDVFWQQFLTENPGSS 360
           V EEFRCKSPGIDMNLEPMATVAP+SVASKDQAAGVNAP+PTG NDVFWQQFLTENPG+S
Sbjct: 301 VREEFRCKSPGIDMNLEPMATVAPDSVASKDQAAGVNAPLPTGFNDVFWQQFLTENPGAS 360

Query: 361 DPQEVQSARKDSDVINEENRQNDHGKFWWNVRNI 395
           DPQEVQSARKDSDVINEENRQ+DHGKFWWN R++
Sbjct: 361 DPQEVQSARKDSDVINEENRQSDHGKFWWNTRSV 393

BLAST of Spg036573 vs. NCBI nr
Match: XP_038895068.1 (heat stress transcription factor A-4a [Benincasa hispida] >XP_038895070.1 heat stress transcription factor A-4a [Benincasa hispida])

HSP 1 Score: 675.6 bits (1742), Expect = 2.9e-190
Identity = 341/394 (86.55%), Postives = 362/394 (91.88%), Query Frame = 0

Query: 1   MDEVQGGGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWNPPEFSSVLLPKFFKH 60
           MDE QGGGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWNP EFSSVLLPKFFKH
Sbjct: 1   MDEAQGGGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWNPLEFSSVLLPKFFKH 60

Query: 61  SNFSSFIRQLNTYGFRKVDPEQWEFANEDFVKGQPHLMRNIHRRKPVHSHSLQNLHGQGI 120
           SNFSSFIRQLNTYGFRKVDPEQWEFAN+DFV+ +PHLM+NIHRRKPVHSHSLQNLHGQGI
Sbjct: 61  SNFSSFIRQLNTYGFRKVDPEQWEFANDDFVRSKPHLMKNIHRRKPVHSHSLQNLHGQGI 120

Query: 121 SPPLTEVERKSLKDDIERLKQDKEQHLLELRKNEQEYQEVNLQMQNLKDRFQLVQQEMQS 180
           S PLTEVER  L DDIERLK DKEQ LLEL+K+EQEYQ V LQMQNLKDRFQ VQQEMQS
Sbjct: 121 S-PLTEVERNGLNDDIERLKLDKEQLLLELQKHEQEYQGVGLQMQNLKDRFQCVQQEMQS 180

Query: 181 FSSWMARLLKKPGLRLDPLPQLETLERKRRLPRVSYNNSEDNVEDNQMGTTQTIGRENMD 240
           F S MAR+L+KPGL LD LPQLET ERKRRLPRVSYNN+ED +EDNQMGTTQTIGR++M 
Sbjct: 181 FISLMARILQKPGLHLDLLPQLETPERKRRLPRVSYNNNEDKLEDNQMGTTQTIGRDDMG 240

Query: 241 CSSDPILKTEQFELVETSLTFWEGIIHSYGQRVSPLDSSSNLELDGSVSHASSPAISCRQ 300
           CS D I K EQFEL+ETSLTFWEGII SYGQ VSPLDSSSNLEL G VSHASSPA SCRQ
Sbjct: 241 CSFDSIFKKEQFELIETSLTFWEGIILSYGQTVSPLDSSSNLELGGCVSHASSPATSCRQ 300

Query: 301 VSEEFRCKSPGIDMNLEPMATVAPESVASKDQAAGVNAPVPTGVNDVFWQQFLTENPGSS 360
           VSEEFRCKSPGIDMNLEP+ TVAP+S+ASKDQ AGVNAPVPTG NDVFWQQFLTENPG+S
Sbjct: 301 VSEEFRCKSPGIDMNLEPVPTVAPDSLASKDQEAGVNAPVPTGANDVFWQQFLTENPGAS 360

Query: 361 DPQEVQSARKDSDVINEENRQNDHGKFWWNVRNI 395
           DPQEVQSARKDSDVIN+ENRQ+DHGKFWWN R++
Sbjct: 361 DPQEVQSARKDSDVINDENRQSDHGKFWWNTRSV 393

BLAST of Spg036573 vs. NCBI nr
Match: XP_023519250.1 (heat stress transcription factor A-4a-like [Cucurbita pepo subsp. pepo] >XP_023519251.1 heat stress transcription factor A-4a-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 672.9 bits (1735), Expect = 1.8e-189
Identity = 337/394 (85.53%), Postives = 361/394 (91.62%), Query Frame = 0

Query: 1   MDEVQGGGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWNPPEFSSVLLPKFFKH 60
           MDE QG GLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVW P EFSSVLLPKFFKH
Sbjct: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60

Query: 61  SNFSSFIRQLNTYGFRKVDPEQWEFANEDFVKGQPHLMRNIHRRKPVHSHSLQNLHGQGI 120
           SNFSSFIRQLNTYGF+KVDP+QWEFAN+DFV+G+ HLM+NIHRRKPVHSHSLQN HGQG+
Sbjct: 61  SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV 120

Query: 121 SPPLTEVERKSLKDDIERLKQDKEQHLLELRKNEQEYQEVNLQMQNLKDRFQLVQQEMQS 180
           SPPLTEVERKS +D+IE LK+DKEQ +LELRK++QEYQ V LQMQNLKDRFQ VQQ MQ 
Sbjct: 121 SPPLTEVERKSFEDNIETLKRDKEQLVLELRKHKQEYQGVVLQMQNLKDRFQCVQQGMQL 180

Query: 181 FSSWMARLLKKPGLRLDPLPQLETLERKRRLPRVSYNNSEDNVEDNQMGTTQTIGRENMD 240
           F S +AR L KPGLRLD LPQLET +RKRRLPRVSYNNSEDN+EDNQMGTTQTI RENMD
Sbjct: 181 FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENMD 240

Query: 241 CSSDPILKTEQFELVETSLTFWEGIIHSYGQRVSPLDSSSNLELDGSVSHASSPAISCRQ 300
           CS DPILK EQFEL+ETS+ FWEGIIHSYGQ +SPLDSSSNLEL GSVSHASSPA++CRQ
Sbjct: 241 CSFDPILKEEQFELLETSIAFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300

Query: 301 VSEEFRCKSPGIDMNLEPMATVAPESVASKDQAAGVNAPVPTGVNDVFWQQFLTENPGSS 360
           VSEE RCKSPGIDMNLEPMATVAPESVASKDQAAGV APVPTGVNDVFWQQFLTENPGSS
Sbjct: 301 VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS 360

Query: 361 DPQEVQSARKDSDVINEENRQNDHGKFWWNVRNI 395
           DPQEVQSARKDSDVI EENR +D G FWWN R++
Sbjct: 361 DPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSV 394

BLAST of Spg036573 vs. ExPASy Swiss-Prot
Match: O49403 (Heat stress transcription factor A-4a OS=Arabidopsis thaliana OX=3702 GN=HSFA4A PE=1 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 1.0e-89
Identity = 192/400 (48.00%), Postives = 259/400 (64.75%), Query Frame = 0

Query: 1   MDEVQGG-GLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWNPPEFSSVLLPKFFK 60
           MDE   G   +SLPPFL KTY+MVDD S++SIVSWS S+KSF+VWNPPEFS  LLP+FFK
Sbjct: 1   MDENNHGVSSSSLPPFLTKTYEMVDDSSSDSIVSWSQSNKSFIVWNPPEFSRDLLPRFFK 60

Query: 61  HSNFSSFIRQLNTYGFRKVDPEQWEFANEDFVKGQPHLMRNIHRRKPVHSHSLQNLHGQG 120
           H+NFSSFIRQLNTYGFRK DPEQWEFAN+DFV+GQPHLM+NIHRRKPVHSHSL NL  Q 
Sbjct: 61  HNNFSSFIRQLNTYGFRKADPEQWEFANDDFVRGQPHLMKNIHRRKPVHSHSLPNLQAQ- 120

Query: 121 ISPPLTEVERKSLKDDIERLKQDKEQHLLELRKNEQEYQEVNLQMQNLKDRFQLVQQEMQ 180
              PLT+ ER  + + IERL ++KE  L EL K ++E +   +Q++ LK+R Q +++  +
Sbjct: 121 -LNPLTDSERVRMNNQIERLTKEKEGLLEELHKQDEEREVFEMQVKELKERLQHMEKRQK 180

Query: 181 SFSSWMARLLKKPGLRLDPLPQL-ETLERKRRLPRVSYNNSEDNVEDNQMGTTQTIGREN 240
           +  S+++++L+KPGL L+  P + ET ERKRR PR+ +   E  +E+N+   T  + RE 
Sbjct: 181 TMVSFVSQVLEKPGLALNLSPCVPETNERKRRFPRIEFFPDEPMLEENK---TCVVVREE 240

Query: 241 MDCSSDPILKTEQFELVETSLTFWEGIIHSYGQRVSPLDSSSNLELDGSVSHASSPAISC 300
              S     +  Q E +E+S+  WE ++    + +    S   L++D S +   SP +SC
Sbjct: 241 GSTSPSSHTREHQVEQLESSIAIWENLVSDSCESMLQSRSMMTLDVDESSTFPESPPLSC 300

Query: 301 RQVSEEFRCKSPG----IDMNLEPMATVAPESVASKDQAAGVNAPVPTGVNDVFWQQFLT 360
            Q+S + R KSP     IDMN EP  +    +VA+         P   G ND FWQQF +
Sbjct: 301 IQLSVDSRLKSPPSPRIIDMNCEPDGSKEQNTVAAPP------PPPVAGANDGFWQQFFS 360

Query: 361 ENPGSSDPQEVQSARKDSDVINEENRQNDHGKFWWNVRNI 395
           ENPGS++ +EVQ  RKD    +++       K WWN RN+
Sbjct: 361 ENPGSTEQREVQLERKD----DKDKAGVRTEKCWWNSRNV 385

BLAST of Spg036573 vs. ExPASy Swiss-Prot
Match: Q9FK72 (Heat stress transcription factor A-4c OS=Arabidopsis thaliana OX=3702 GN=HSFA4C PE=2 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 1.2e-82
Identity = 189/395 (47.85%), Postives = 247/395 (62.53%), Query Frame = 0

Query: 1   MDEVQGGGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWNPPEFSSVLLPKFFKH 60
           MDE   GG +SLPPFL KTY+MVDD S++S+V+WS ++KSF+V NP EFS  LLP+FFKH
Sbjct: 1   MDE-NNGGSSSLPPFLTKTYEMVDDSSSDSVVAWSENNKSFIVKNPAEFSRDLLPRFFKH 60

Query: 61  SNFSSFIRQLNTYGFRKVDPEQWEFANEDFVKGQPHLMRNIHRRKPVHSHSLQNLHGQGI 120
            NFSSFIRQLNTYGFRKVDPE+WEF N+DFV+G+P+LM+NIHRRKPVHSHSL NL  Q  
Sbjct: 61  KNFSSFIRQLNTYGFRKVDPEKWEFLNDDFVRGRPYLMKNIHRRKPVHSHSLVNLQAQN- 120

Query: 121 SPPLTEVERKSLKDDIERLKQDKEQHLLELRKNEQEYQEVNLQMQNLKDRFQLVQQEMQS 180
             PLTE ER+S++D IERLK +KE  L EL+  EQE +E  LQ+  LKDR Q ++Q  +S
Sbjct: 121 --PLTESERRSMEDQIERLKNEKEGLLAELQNQEQERKEFELQVTTLKDRLQHMEQHQKS 180

Query: 181 FSSWMARLLKKPGLRLDPLPQLETLERKRRLPRVSYNNSEDNVEDNQMGTTQTIGRENMD 240
             ++++++L KPGL L+    LE  ER++R             ++N +  + +       
Sbjct: 181 IVAYVSQVLGKPGLSLN----LENHERRKR-----------RFQENSLPPSSS------- 240

Query: 241 CSSDPILKTEQFELVETSLTFWEGIIHSYGQRVSPLDSSSNLELDGSVSHASSPAISCRQ 300
                    EQ E +E+SLTFWE ++    ++ S L SSS ++ D + S  S        
Sbjct: 241 -------HIEQVEKLESSLTFWENLVSESCEK-SGLQSSS-MDHDAAESSLS-------- 300

Query: 301 VSEEFRCKSPGIDMNLEPMATVAPESVASKDQAAGVNAPVP-TGVNDVFWQQFLTENPGS 360
              + R KS  IDMN EP  T              V AP P TGVND FW+Q LTENPGS
Sbjct: 301 -IGDTRPKSSKIDMNSEPPVT--------------VTAPAPKTGVNDDFWEQCLTENPGS 337

Query: 361 SDPQEVQSARKDSDVINEENRQNDHGKFWWNVRNI 395
           ++ QEVQS R+D    N  N+  +   +WWN  N+
Sbjct: 361 TEQQEVQSERRDVGNDNNGNKIGNQRTYWWNSGNV 337

BLAST of Spg036573 vs. ExPASy Swiss-Prot
Match: Q94J16 (Heat stress transcription factor A-4b OS=Oryza sativa subsp. japonica OX=39947 GN=HSFA4B PE=2 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 8.6e-73
Identity = 180/428 (42.06%), Postives = 241/428 (56.31%), Query Frame = 0

Query: 6   GGGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWNPPEFSSVLLPKFFKHSNFSS 65
           GGG  SLPPFL KTY+MVDDPST+++V W+ +  SFVV N PEF   LLPK+FKH+NFSS
Sbjct: 4   GGGGGSLPPFLSKTYEMVDDPSTDAVVGWTPAGTSFVVANQPEFCRDLLPKYFKHNNFSS 63

Query: 66  FIRQLNTYGFRKVDPEQWEFANEDFVKGQPHLMRNIHRRKPVHSHSLQNLHGQGISPPLT 125
           F+RQLNTYGFRKVDPEQWEFANEDF+KGQ H ++NIHRRKP+ SHS    H QG + PLT
Sbjct: 64  FVRQLNTYGFRKVDPEQWEFANEDFIKGQRHRLKNIHRRKPIFSHS---SHSQG-AGPLT 123

Query: 126 EVERKSLKDDIERLKQDKEQHLLELRKNEQEYQEVNLQMQNLKDRFQLVQQEMQSFSSWM 185
           + ERK  +++IERLK D      EL+ N  +   +  +MQ L+++  +V+ + +S  S++
Sbjct: 124 DNERKDYEEEIERLKSDNAALSSELQNNTLKKLNMEKRMQALEEKLFVVEDQQRSLISYV 183

Query: 186 ARLLKKPGLRLDPLPQLETLERKRRLPRVSYNNSEDNVEDNQM-------GTTQTIGREN 245
             ++K PG     + Q +   +KRRLP     + + N ++NQ+          QT  RE+
Sbjct: 184 REIVKAPGFLSSFVQQQDHHRKKRRLPIPISFHEDANTQENQIMPCDLTNSPAQTFYRES 243

Query: 246 MDCSSDPILKTEQF-----ELVETSLTFWEGI-----------IHSYGQ---RVSPL--- 305
            D     +   E F     E     +++ +G+           +HS G+   RVS     
Sbjct: 244 FDKMESSLNSLENFLREASEEFGNDISYDDGVPGPSSTVVLTELHSPGESDPRVSSPPTR 303

Query: 306 ---------DSSSNLELDGSVSHASSPAISCRQVSEEFRCKSPGIDMNLEPMATVAPESV 365
                    DS S+ ++  S S A SP I       + R K   ID+N EP  T   E+ 
Sbjct: 304 MRTSSAGAGDSHSSRDVAESTSCAESPPIPQMHSRVDTRAKVSEIDVNSEPAVT---ETG 363

Query: 366 ASKDQAAGVNAPVPTGVNDVFWQQFLTENPGSSDP-QEVQSARKDSDVINEENRQNDHGK 395
            S+DQ A     V  G ND FWQQFLTE PGSSD  QE QS R+D     +E +  D   
Sbjct: 364 PSRDQPAEEPPAVTPGANDGFWQQFLTEQPGSSDAHQEAQSERRDGGNKVDEMKSGDRQH 423

BLAST of Spg036573 vs. ExPASy Swiss-Prot
Match: Q93VB5 (Heat stress transcription factor A-4d OS=Oryza sativa subsp. japonica OX=39947 GN=HSFA4D PE=1 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 6.0e-58
Identity = 157/418 (37.56%), Postives = 230/418 (55.02%), Query Frame = 0

Query: 6   GGGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWNPPEFSSVLLPKFFKHSNFSS 65
           GGG    PPFL+KTY+MV+D +TN +VSW     SFVVWNP +FS  LLPK+FKH+NFSS
Sbjct: 12  GGGGGGPPPFLIKTYEMVEDAATNHVVSWGPGGASFVVWNPLDFSRDLLPKYFKHNNFSS 71

Query: 66  FIRQLNTYGFRKVDPEQWEFANEDFVKGQPHLMRNIHRRKPVHSHSLQNLHGQGISPPLT 125
           FIRQLNTYGFRK+DPE+WEFANEDF++G  HL++NIHRRKPVHSHSLQN     I+ PL 
Sbjct: 72  FIRQLNTYGFRKIDPERWEFANEDFIRGHTHLLKNIHRRKPVHSHSLQN----QINGPLA 131

Query: 126 EVERKSLKDDIERLKQDKEQHLLELRKNEQEYQEVNLQMQNLKDRFQLVQQEMQSFSSWM 185
           E ER+ L+++I RLK +K   + +L++  Q+   +N QMQ ++ R   ++Q  ++  + +
Sbjct: 132 ESERRELEEEINRLKYEKSILVADLQRQNQQQYVINWQMQAMEGRLVAMEQRQKNIVASL 191

Query: 186 ARLLKKPGLRL-DPLPQLETLERKRRLPRVS-YNNSEDNVEDNQMGTTQTIGRENMDCSS 245
             +L++ G  +   L + +   +KRR+P++  + +     E+ ++   Q IG +      
Sbjct: 192 CEMLQRRGGAVSSSLLESDHFSKKRRVPKMDLFVDDCAAGEEQKVFQFQGIGTDAPAMPP 251

Query: 246 D-PILKTEQFELVETSLTFWEGII---------------HSYG------------QRVSP 305
             P+   E F+ VE SL   E +                H +G               +P
Sbjct: 252 VLPVTNGEAFDRVELSLVSLEKLFQRANDACTAAEEMYSHGHGGTEPSTAICPEEMNTAP 311

Query: 306 LDSSSNLELDGSVSHASSP--AISCRQVSEEFRCKSPGIDMNLE-PMATVAPE-SVASKD 365
           +++  +L+L  S+ H SSP    +   +S E   +SPG   + E PMA +  +  V    
Sbjct: 312 METGIDLQLPASL-HPSSPNTGNAHLHLSTEL-TESPGFVQSPELPMAEIREDIHVTRYP 371

Query: 366 QAAGVNAPVPTG-----------------VNDVFWQQFLTENPGSS-DPQEVQSARKD 372
             A VN+ + +                   NDVFW++FLTE P S  D  E Q + KD
Sbjct: 372 TQADVNSEIASSTDTSQDGTSETEASHGPTNDVFWERFLTETPRSCLDESERQESPKD 423

BLAST of Spg036573 vs. ExPASy Swiss-Prot
Match: Q94BZ5 (Heat stress transcription factor A-5 OS=Arabidopsis thaliana OX=3702 GN=HSFA5 PE=2 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 9.3e-51
Identity = 150/447 (33.56%), Postives = 224/447 (50.11%), Query Frame = 0

Query: 6   GGGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWNPPEFSSVLLPKFFKHSNFSS 65
           G G     PFLVKTY+MVDD ST+ IVSWS+++ SF+VWN  EFS +LLP +FKH+NFSS
Sbjct: 15  GEGAGGPAPFLVKTYEMVDDSSTDQIVSWSANNNSFIVWNHAEFSRLLLPTYFKHNNFSS 74

Query: 66  FIRQLNTYGFRKVDPEQWEFANEDFVKGQPHLMRNIHRRKPVHSHSLQNLHGQGISPPL- 125
           FIRQLNTYGFRK+DPE+WEF N+DF+K Q HL++NIHRRKP+HSHS          PP  
Sbjct: 75  FIRQLNTYGFRKIDPERWEFLNDDFIKDQKHLLKNIHRRKPIHSHS---------HPPAS 134

Query: 126 -TEVERKSLKDDIERLKQDKEQHLLELRKNEQEYQEVNLQMQNLKDRFQLVQQEMQSFSS 185
            T+ ER  L++ +++L ++K     +L K +Q+      Q + + +    ++   +   +
Sbjct: 135 STDQERAVLQEQMDKLSREKAAIEAKLLKFKQQKVVAKHQFEEMTEHVDDMENRQKKLLN 194

Query: 186 WMARLLKKP------GLRLDPLPQLETLERKRRLPRVSYNN--SEDNVEDNQMGTTQ--- 245
           ++   ++ P      G +++ L  +    +KRRLP V  +   SED+  DN  G+++   
Sbjct: 195 FLETAIRNPTFVKNFGKKVEQL-DISAYNKKRRLPEVEQSKPPSEDSHLDNSSGSSRRES 254

Query: 246 ----------------------------TIGRENMDCSS--------DP----------I 305
                                       +I   N + +S        DP           
Sbjct: 255 GNIFHQNFSNKLRLELSPADSDMNMVSHSIQSSNEEGASPKGILSGGDPNTTLTKREGLP 314

Query: 306 LKTEQFELVETSLTFWEGIIHSYGQRVSPLDS--SSNLELDGSVS-----HASSPAISCR 365
              E  EL +T  T    ++ +   RV  L    +S+ E DGS S       +S  +  +
Sbjct: 315 FAPEALELADTG-TCPRRLLLNDNTRVETLQQRLTSSEETDGSFSCHLNLTLASAPLPDK 374

Query: 366 QVSEEFRCKSPGIDMNLEPMATVAPESVASKDQAA----GVNAPVPTGVNDVFWQQFLTE 383
             S+  +      ++N   + T A E    + + A      NA  P  VNDVFW+QFLTE
Sbjct: 375 TASQIAKTTLKSQELNFNSIETSASEKNRGRQEIAVGGSQANAAPPARVNDVFWEQFLTE 434

BLAST of Spg036573 vs. ExPASy TrEMBL
Match: A0A6J1EI67 (heat stress transcription factor A-4c-like OS=Cucurbita moschata OX=3662 GN=LOC111434310 PE=3 SV=1)

HSP 1 Score: 677.2 bits (1746), Expect = 4.7e-191
Identity = 341/394 (86.55%), Postives = 361/394 (91.62%), Query Frame = 0

Query: 1   MDEVQGGGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWNPPEFSSVLLPKFFKH 60
           MDE QG GLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVW P EFSSVLLPKFFKH
Sbjct: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60

Query: 61  SNFSSFIRQLNTYGFRKVDPEQWEFANEDFVKGQPHLMRNIHRRKPVHSHSLQNLHGQGI 120
           SNFSSFIRQLNTYGF+KVDP+QWEFAN+DFV+G+ HLM+NIHRRKPVHSHSLQN HGQG+
Sbjct: 61  SNFSSFIRQLNTYGFKKVDPDQWEFANDDFVRGEQHLMKNIHRRKPVHSHSLQNPHGQGV 120

Query: 121 SPPLTEVERKSLKDDIERLKQDKEQHLLELRKNEQEYQEVNLQMQNLKDRFQLVQQEMQS 180
           SPPLTEVERKS +D+IE LK+DKEQ LLELRK+EQEYQ V LQMQNLKDRFQ VQQ MQ 
Sbjct: 121 SPPLTEVERKSFEDNIETLKRDKEQLLLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQL 180

Query: 181 FSSWMARLLKKPGLRLDPLPQLETLERKRRLPRVSYNNSEDNVEDNQMGTTQTIGRENMD 240
           F S +AR L KPGLRLD LPQLET +RKRRLPRVSYNNSEDN+EDNQMGTTQTI REN D
Sbjct: 181 FISLIARFLHKPGLRLDLLPQLETSDRKRRLPRVSYNNSEDNLEDNQMGTTQTISRENRD 240

Query: 241 CSSDPILKTEQFELVETSLTFWEGIIHSYGQRVSPLDSSSNLELDGSVSHASSPAISCRQ 300
           CS DPILK EQFELVETSLTFWEGIIHSYGQ +SPLDSSSNLEL GSVSHASSPA++CRQ
Sbjct: 241 CSFDPILKEEQFELVETSLTFWEGIIHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300

Query: 301 VSEEFRCKSPGIDMNLEPMATVAPESVASKDQAAGVNAPVPTGVNDVFWQQFLTENPGSS 360
           VSEE RCKSPGIDMNLEPMATVAPESVASKDQAAGV APVPTGVNDVFWQQFLTENPGSS
Sbjct: 301 VSEELRCKSPGIDMNLEPMATVAPESVASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS 360

Query: 361 DPQEVQSARKDSDVINEENRQNDHGKFWWNVRNI 395
           DPQEVQSARKDSDVI EENR +D G FWWN R++
Sbjct: 361 DPQEVQSARKDSDVIIEENRPSDQGNFWWNTRSV 394

BLAST of Spg036573 vs. ExPASy TrEMBL
Match: A0A0A0LV22 (HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G467710 PE=3 SV=1)

HSP 1 Score: 676.0 bits (1743), Expect = 1.1e-190
Identity = 343/394 (87.06%), Postives = 364/394 (92.39%), Query Frame = 0

Query: 1   MDEVQGGGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWNPPEFSSVLLPKFFKH 60
           MDE QGGGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWNP EFSSVLLPKFFKH
Sbjct: 1   MDEAQGGGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWNPLEFSSVLLPKFFKH 60

Query: 61  SNFSSFIRQLNTYGFRKVDPEQWEFANEDFVKGQPHLMRNIHRRKPVHSHSLQNLHGQGI 120
           SNFSSFIRQLNTYGFRKVDPEQWEFANEDFV+G+PHLM+NIHRRKP+HSHSLQNLHGQGI
Sbjct: 61  SNFSSFIRQLNTYGFRKVDPEQWEFANEDFVRGKPHLMKNIHRRKPIHSHSLQNLHGQGI 120

Query: 121 SPPLTEVERKSLKDDIERLKQDKEQHLLELRKNEQEYQEVNLQMQNLKDRFQLVQQEMQS 180
           S PLTEVER S KDDIERLK DKEQ LLEL+K EQEYQ V LQ+QNLKDRFQ VQQEMQ 
Sbjct: 121 S-PLTEVERNSFKDDIERLKLDKEQLLLELQKYEQEYQGVGLQIQNLKDRFQRVQQEMQL 180

Query: 181 FSSWMARLLKKPGLRLDPLPQLETLERKRRLPRVSYNNSEDNVEDNQMGTTQTIGRENMD 240
           F S MARLL+KPGL LD LPQLET ERKRRLPRVSYN SED++EDN +GTTQTIGR++M 
Sbjct: 181 FISLMARLLQKPGLHLDLLPQLETPERKRRLPRVSYNISEDSLEDNHLGTTQTIGRDDMG 240

Query: 241 CSSDPILKTEQFELVETSLTFWEGIIHSYGQRVSPLDSSSNLELDGSVSHASSPAISCRQ 300
           CS DPIL+ EQ EL+ETSLTFWEGIIHSY + VSPLDSSSNLEL GSVSHASSPAISCR 
Sbjct: 241 CSFDPILEKEQLELLETSLTFWEGIIHSYDETVSPLDSSSNLELVGSVSHASSPAISCRL 300

Query: 301 VSEEFRCKSPGIDMNLEPMATVAPESVASKDQAAGVNAPVPTGVNDVFWQQFLTENPGSS 360
           V EEFRCKSPGIDMNLEPMATVAP+SVASKDQAAGVNAP+PTG NDVFWQQFLTENPG+S
Sbjct: 301 VREEFRCKSPGIDMNLEPMATVAPDSVASKDQAAGVNAPLPTGFNDVFWQQFLTENPGAS 360

Query: 361 DPQEVQSARKDSDVINEENRQNDHGKFWWNVRNI 395
           DPQEVQSARKDSDVINEENRQ+DHGKFWWN R++
Sbjct: 361 DPQEVQSARKDSDVINEENRQSDHGKFWWNTRSV 393

BLAST of Spg036573 vs. ExPASy TrEMBL
Match: A0A6J1KEK1 (heat stress transcription factor A-4a-like OS=Cucurbita maxima OX=3661 GN=LOC111495117 PE=3 SV=1)

HSP 1 Score: 669.8 bits (1727), Expect = 7.6e-189
Identity = 338/388 (87.11%), Postives = 357/388 (92.01%), Query Frame = 0

Query: 1   MDEVQGGGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWNPPEFSSVLLPKFFKH 60
           MDE QG GLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVW P EFSSVLLPKFFKH
Sbjct: 1   MDEAQGSGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWKPLEFSSVLLPKFFKH 60

Query: 61  SNFSSFIRQLNTYGFRKVDPEQWEFANEDFVKGQPHLMRNIHRRKPVHSHSLQNLHGQGI 120
           SNFSSFIRQLNTYGF+KV PEQWEFAN+DFV+G+ HLM+NIHRRKPVHSHSLQN HGQG+
Sbjct: 61  SNFSSFIRQLNTYGFKKVHPEQWEFANDDFVRGKQHLMKNIHRRKPVHSHSLQNPHGQGV 120

Query: 121 SPPLTEVERKSLKDDIERLKQDKEQHLLELRKNEQEYQEVNLQMQNLKDRFQLVQQEMQS 180
           SPPLTEVERKS +DDIE LK+DKEQ LLELRK+EQEYQ V LQMQNLKDRFQ VQQ MQ 
Sbjct: 121 SPPLTEVERKSFEDDIETLKRDKEQILLELRKHEQEYQGVVLQMQNLKDRFQCVQQGMQL 180

Query: 181 FSSWMARLLKKPGLRLDPLPQLETLERKRRLPRVSYNNSEDNVEDNQMGTTQTIGRENMD 240
           F S +ARLL KPG RLD LPQLET +RKRRLPRVSYNNSEDN+EDNQMGTTQTI RENMD
Sbjct: 181 FISLIARLLHKPGRRLDLLPQLETSDRKRRLPRVSYNNSEDNIEDNQMGTTQTISRENMD 240

Query: 241 CSSDPILKTEQFELVETSLTFWEGIIHSYGQRVSPLDSSSNLELDGSVSHASSPAISCRQ 300
           CS DPILK EQFELVETSLTFWEGI+HSYGQ +SPLDSSSNLEL GSVSHASSPA++CRQ
Sbjct: 241 CSFDPILKEEQFELVETSLTFWEGIVHSYGQTISPLDSSSNLELGGSVSHASSPAVTCRQ 300

Query: 301 VSEEFRCKSPGIDMNLEPMATVAPESVASKDQAAGVNAPVPTGVNDVFWQQFLTENPGSS 360
           VSEE RCKSPGIDMNLEPMATVAPES+ASKDQAAGV APVPTGVNDVFWQQFLTENPGSS
Sbjct: 301 VSEELRCKSPGIDMNLEPMATVAPESIASKDQAAGVKAPVPTGVNDVFWQQFLTENPGSS 360

Query: 361 DPQEVQSARKDSDVINEENRQNDHGKFW 389
           DPQEVQSARKDSDVI EENR++D G FW
Sbjct: 361 DPQEVQSARKDSDVIIEENRRSDQGNFW 388

BLAST of Spg036573 vs. ExPASy TrEMBL
Match: A0A1S4DVL4 (heat stress transcription factor A-4c OS=Cucumis melo OX=3656 GN=LOC103487107 PE=3 SV=1)

HSP 1 Score: 661.4 bits (1705), Expect = 2.7e-186
Identity = 336/394 (85.28%), Postives = 358/394 (90.86%), Query Frame = 0

Query: 1   MDEVQGGGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWNPPEFSSVLLPKFFKH 60
           MDE QGGGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWNP EFSSVLLPKFFKH
Sbjct: 1   MDEAQGGGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWNPLEFSSVLLPKFFKH 60

Query: 61  SNFSSFIRQLNTYGFRKVDPEQWEFANEDFVKGQPHLMRNIHRRKPVHSHSLQNLHGQGI 120
           SNFSSFIRQLNTYGFRKVDPEQWEFANEDFV+G+PHLM+NIHRRKP+HSHSLQNLHGQGI
Sbjct: 61  SNFSSFIRQLNTYGFRKVDPEQWEFANEDFVRGKPHLMKNIHRRKPIHSHSLQNLHGQGI 120

Query: 121 SPPLTEVERKSLKDDIERLKQDKEQHLLELRKNEQEYQEVNLQMQNLKDRFQLVQQEMQS 180
           S PLTEVER S KD+IERLK DKEQ LLEL+K EQEYQ V LQMQNLKDRFQ VQQ MQ 
Sbjct: 121 S-PLTEVERNSFKDNIERLKLDKEQLLLELQKYEQEYQGVGLQMQNLKDRFQRVQQGMQL 180

Query: 181 FSSWMARLLKKPGLRLDPLPQLETLERKRRLPRVSYNNSEDNVEDNQMGTTQTIGRENMD 240
           F   MARL +KPGLRLD LPQLET ERKRRLPR SYN SED++ED+Q+GTTQ IGRE++ 
Sbjct: 181 FIGLMARLFQKPGLRLDLLPQLETPERKRRLPRASYNISEDSLEDDQLGTTQAIGREDLS 240

Query: 241 CSSDPILKTEQFELVETSLTFWEGIIHSYGQRVSPLDSSSNLELDGSVSHASSPAISCRQ 300
           CS DPIL+ EQ EL+ETSLTFWEGIIHSY Q V PLDSSSNLEL GSVSHASSPAISCR 
Sbjct: 241 CSFDPILEKEQLELLETSLTFWEGIIHSYDQTVIPLDSSSNLELVGSVSHASSPAISCRL 300

Query: 301 VSEEFRCKSPGIDMNLEPMATVAPESVASKDQAAGVNAPVPTGVNDVFWQQFLTENPGSS 360
           V EEFRCKSPGIDMNLEPMATVAP+SVASKDQAAGVNAP+PTG NDVFWQQFLTENPG+S
Sbjct: 301 VREEFRCKSPGIDMNLEPMATVAPDSVASKDQAAGVNAPLPTGFNDVFWQQFLTENPGAS 360

Query: 361 DPQEVQSARKDSDVINEENRQNDHGKFWWNVRNI 395
           DPQEVQSARKDSDVINEEN+Q+DH  FWWN R++
Sbjct: 361 DPQEVQSARKDSDVINEENKQSDHENFWWNTRSV 393

BLAST of Spg036573 vs. ExPASy TrEMBL
Match: A0A5A7UU40 (Heat stress transcription factor A-4c OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold96G002540 PE=3 SV=1)

HSP 1 Score: 641.3 bits (1653), Expect = 2.9e-180
Identity = 329/383 (85.90%), Postives = 350/383 (91.38%), Query Frame = 0

Query: 1   MDEVQGGGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWNPPEFSSVLLPKFFKH 60
           MDE QGGGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWNP EFSSVLLPKFFKH
Sbjct: 1   MDEAQGGGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWNPLEFSSVLLPKFFKH 60

Query: 61  SNFSSFIRQLNTYGFRKVDPEQWEFANEDFVKGQPHLMRNIHRRKPVHSHSLQNLHGQGI 120
           SNFSSFIRQLNTYGFRKVDPEQWEFANEDFV+G+PHLM+NIHRRKP+HSHSLQNLHGQGI
Sbjct: 61  SNFSSFIRQLNTYGFRKVDPEQWEFANEDFVRGKPHLMKNIHRRKPIHSHSLQNLHGQGI 120

Query: 121 SPPLTEVERKSLKDDIERLKQDKEQHLLELRKNEQEYQEVNLQMQNLKDRFQLVQQEMQS 180
           S PLTEVER S KD+IERLK DKEQ LLEL+K EQEYQ V LQMQNLKDRFQ VQQ MQ 
Sbjct: 121 S-PLTEVERNSFKDNIERLKLDKEQLLLELQKYEQEYQGVGLQMQNLKDRFQRVQQGMQL 180

Query: 181 FSSWMARLLKKPGLRLDPLPQLETLERKRRLPRVSYNNSEDNVEDNQMGTTQTIGRENMD 240
           F   MARL +KPGLRLD LPQLET ERKRRLPR SYN SED++ED+Q+GTTQ IGRE++ 
Sbjct: 181 FIGLMARLFQKPGLRLDLLPQLETPERKRRLPRASYNISEDSLEDDQLGTTQAIGREDLS 240

Query: 241 CSSDPILKTEQFELVETSLTFWEGIIHSYGQRVSPLDSSSNLELDGSVSHASSPAISCRQ 300
           CS DPIL+ EQ EL+ETSLTFWEGIIHSY Q V PLDSSSNLEL GSVSHASSPAISCR 
Sbjct: 241 CSFDPILEKEQLELLETSLTFWEGIIHSYDQTVIPLDSSSNLELVGSVSHASSPAISCRL 300

Query: 301 VSEEFRCKSPGIDMNLEPMATVAPESVASKDQAAGVNAPVPTGVNDVFWQQFLTENPGSS 360
           V EEFRCKSPGIDMNLEPMATVAP+SVASKDQAAGVNAP+PTG NDVFWQQFLTENPG+S
Sbjct: 301 VREEFRCKSPGIDMNLEPMATVAPDSVASKDQAAGVNAPLPTGFNDVFWQQFLTENPGAS 360

Query: 361 DPQEVQSARKDSDVINEENRQND 384
           DPQEVQSARKDSDVINEEN+Q++
Sbjct: 361 DPQEVQSARKDSDVINEENKQSE 382

BLAST of Spg036573 vs. TAIR 10
Match: AT4G18880.1 (heat shock transcription factor A4A )

HSP 1 Score: 332.0 bits (850), Expect = 7.2e-91
Identity = 192/400 (48.00%), Postives = 259/400 (64.75%), Query Frame = 0

Query: 1   MDEVQGG-GLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWNPPEFSSVLLPKFFK 60
           MDE   G   +SLPPFL KTY+MVDD S++SIVSWS S+KSF+VWNPPEFS  LLP+FFK
Sbjct: 1   MDENNHGVSSSSLPPFLTKTYEMVDDSSSDSIVSWSQSNKSFIVWNPPEFSRDLLPRFFK 60

Query: 61  HSNFSSFIRQLNTYGFRKVDPEQWEFANEDFVKGQPHLMRNIHRRKPVHSHSLQNLHGQG 120
           H+NFSSFIRQLNTYGFRK DPEQWEFAN+DFV+GQPHLM+NIHRRKPVHSHSL NL  Q 
Sbjct: 61  HNNFSSFIRQLNTYGFRKADPEQWEFANDDFVRGQPHLMKNIHRRKPVHSHSLPNLQAQ- 120

Query: 121 ISPPLTEVERKSLKDDIERLKQDKEQHLLELRKNEQEYQEVNLQMQNLKDRFQLVQQEMQ 180
              PLT+ ER  + + IERL ++KE  L EL K ++E +   +Q++ LK+R Q +++  +
Sbjct: 121 -LNPLTDSERVRMNNQIERLTKEKEGLLEELHKQDEEREVFEMQVKELKERLQHMEKRQK 180

Query: 181 SFSSWMARLLKKPGLRLDPLPQL-ETLERKRRLPRVSYNNSEDNVEDNQMGTTQTIGREN 240
           +  S+++++L+KPGL L+  P + ET ERKRR PR+ +   E  +E+N+   T  + RE 
Sbjct: 181 TMVSFVSQVLEKPGLALNLSPCVPETNERKRRFPRIEFFPDEPMLEENK---TCVVVREE 240

Query: 241 MDCSSDPILKTEQFELVETSLTFWEGIIHSYGQRVSPLDSSSNLELDGSVSHASSPAISC 300
              S     +  Q E +E+S+  WE ++    + +    S   L++D S +   SP +SC
Sbjct: 241 GSTSPSSHTREHQVEQLESSIAIWENLVSDSCESMLQSRSMMTLDVDESSTFPESPPLSC 300

Query: 301 RQVSEEFRCKSPG----IDMNLEPMATVAPESVASKDQAAGVNAPVPTGVNDVFWQQFLT 360
            Q+S + R KSP     IDMN EP  +    +VA+         P   G ND FWQQF +
Sbjct: 301 IQLSVDSRLKSPPSPRIIDMNCEPDGSKEQNTVAAPP------PPPVAGANDGFWQQFFS 360

Query: 361 ENPGSSDPQEVQSARKDSDVINEENRQNDHGKFWWNVRNI 395
           ENPGS++ +EVQ  RKD    +++       K WWN RN+
Sbjct: 361 ENPGSTEQREVQLERKD----DKDKAGVRTEKCWWNSRNV 385

BLAST of Spg036573 vs. TAIR 10
Match: AT5G45710.1 (winged-helix DNA-binding transcription factor family protein )

HSP 1 Score: 308.5 bits (789), Expect = 8.5e-84
Identity = 189/395 (47.85%), Postives = 247/395 (62.53%), Query Frame = 0

Query: 1   MDEVQGGGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWNPPEFSSVLLPKFFKH 60
           MDE   GG +SLPPFL KTY+MVDD S++S+V+WS ++KSF+V NP EFS  LLP+FFKH
Sbjct: 1   MDE-NNGGSSSLPPFLTKTYEMVDDSSSDSVVAWSENNKSFIVKNPAEFSRDLLPRFFKH 60

Query: 61  SNFSSFIRQLNTYGFRKVDPEQWEFANEDFVKGQPHLMRNIHRRKPVHSHSLQNLHGQGI 120
            NFSSFIRQLNTYGFRKVDPE+WEF N+DFV+G+P+LM+NIHRRKPVHSHSL NL  Q  
Sbjct: 61  KNFSSFIRQLNTYGFRKVDPEKWEFLNDDFVRGRPYLMKNIHRRKPVHSHSLVNLQAQN- 120

Query: 121 SPPLTEVERKSLKDDIERLKQDKEQHLLELRKNEQEYQEVNLQMQNLKDRFQLVQQEMQS 180
             PLTE ER+S++D IERLK +KE  L EL+  EQE +E  LQ+  LKDR Q ++Q  +S
Sbjct: 121 --PLTESERRSMEDQIERLKNEKEGLLAELQNQEQERKEFELQVTTLKDRLQHMEQHQKS 180

Query: 181 FSSWMARLLKKPGLRLDPLPQLETLERKRRLPRVSYNNSEDNVEDNQMGTTQTIGRENMD 240
             ++++++L KPGL L+    LE  ER++R             ++N +  + +       
Sbjct: 181 IVAYVSQVLGKPGLSLN----LENHERRKR-----------RFQENSLPPSSS------- 240

Query: 241 CSSDPILKTEQFELVETSLTFWEGIIHSYGQRVSPLDSSSNLELDGSVSHASSPAISCRQ 300
                    EQ E +E+SLTFWE ++    ++ S L SSS ++ D + S  S        
Sbjct: 241 -------HIEQVEKLESSLTFWENLVSESCEK-SGLQSSS-MDHDAAESSLS-------- 300

Query: 301 VSEEFRCKSPGIDMNLEPMATVAPESVASKDQAAGVNAPVP-TGVNDVFWQQFLTENPGS 360
              + R KS  IDMN EP  T              V AP P TGVND FW+Q LTENPGS
Sbjct: 301 -IGDTRPKSSKIDMNSEPPVT--------------VTAPAPKTGVNDDFWEQCLTENPGS 337

Query: 361 SDPQEVQSARKDSDVINEENRQNDHGKFWWNVRNI 395
           ++ QEVQS R+D    N  N+  +   +WWN  N+
Sbjct: 361 TEQQEVQSERRDVGNDNNGNKIGNQRTYWWNSGNV 337

BLAST of Spg036573 vs. TAIR 10
Match: AT4G13980.1 (winged-helix DNA-binding transcription factor family protein )

HSP 1 Score: 202.6 bits (514), Expect = 6.6e-52
Identity = 150/447 (33.56%), Postives = 224/447 (50.11%), Query Frame = 0

Query: 6   GGGLTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWNPPEFSSVLLPKFFKHSNFSS 65
           G G     PFLVKTY+MVDD ST+ IVSWS+++ SF+VWN  EFS +LLP +FKH+NFSS
Sbjct: 15  GEGAGGPAPFLVKTYEMVDDSSTDQIVSWSANNNSFIVWNHAEFSRLLLPTYFKHNNFSS 74

Query: 66  FIRQLNTYGFRKVDPEQWEFANEDFVKGQPHLMRNIHRRKPVHSHSLQNLHGQGISPPL- 125
           FIRQLNTYGFRK+DPE+WEF N+DF+K Q HL++NIHRRKP+HSHS          PP  
Sbjct: 75  FIRQLNTYGFRKIDPERWEFLNDDFIKDQKHLLKNIHRRKPIHSHS---------HPPAS 134

Query: 126 -TEVERKSLKDDIERLKQDKEQHLLELRKNEQEYQEVNLQMQNLKDRFQLVQQEMQSFSS 185
            T+ ER  L++ +++L ++K     +L K +Q+      Q + + +    ++   +   +
Sbjct: 135 STDQERAVLQEQMDKLSREKAAIEAKLLKFKQQKVVAKHQFEEMTEHVDDMENRQKKLLN 194

Query: 186 WMARLLKKP------GLRLDPLPQLETLERKRRLPRVSYNN--SEDNVEDNQMGTTQ--- 245
           ++   ++ P      G +++ L  +    +KRRLP V  +   SED+  DN  G+++   
Sbjct: 195 FLETAIRNPTFVKNFGKKVEQL-DISAYNKKRRLPEVEQSKPPSEDSHLDNSSGSSRRES 254

Query: 246 ----------------------------TIGRENMDCSS--------DP----------I 305
                                       +I   N + +S        DP           
Sbjct: 255 GNIFHQNFSNKLRLELSPADSDMNMVSHSIQSSNEEGASPKGILSGGDPNTTLTKREGLP 314

Query: 306 LKTEQFELVETSLTFWEGIIHSYGQRVSPLDS--SSNLELDGSVS-----HASSPAISCR 365
              E  EL +T  T    ++ +   RV  L    +S+ E DGS S       +S  +  +
Sbjct: 315 FAPEALELADTG-TCPRRLLLNDNTRVETLQQRLTSSEETDGSFSCHLNLTLASAPLPDK 374

Query: 366 QVSEEFRCKSPGIDMNLEPMATVAPESVASKDQAA----GVNAPVPTGVNDVFWQQFLTE 383
             S+  +      ++N   + T A E    + + A      NA  P  VNDVFW+QFLTE
Sbjct: 375 TASQIAKTTLKSQELNFNSIETSASEKNRGRQEIAVGGSQANAAPPARVNDVFWEQFLTE 434

BLAST of Spg036573 vs. TAIR 10
Match: AT5G16820.1 (heat shock factor 3 )

HSP 1 Score: 194.1 bits (492), Expect = 2.3e-49
Identity = 107/225 (47.56%), Postives = 147/225 (65.33%), Query Frame = 0

Query: 9   LTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWNPPEFSSVLLPKFFKHSNFSSFIR 68
           + S+PPFL KTYDMVDDP TN +VSWSS + SFVVW+ PEFS VLLPK+FKH+NFSSF+R
Sbjct: 22  VNSVPPFLSKTYDMVDDPLTNEVVSWSSGNNSFVVWSAPEFSKVLLPKYFKHNNFSSFVR 81

Query: 69  QLNTYGFRKVDPEQWEFANEDFVKGQPHLMRNIHRRKPVH-SHSLQNLHGQGIS-PPLTE 128
           QLNTYGFRKVDP++WEFANE F++G+  L+++I RRKP H   + Q    Q  S     E
Sbjct: 82  QLNTYGFRKVDPDRWEFANEGFLRGRKQLLKSIVRRKPSHVQQNQQQTQVQSSSVGACVE 141

Query: 129 VERKSLKDDIERLKQDKEQHLLELRKNEQEYQEVNLQMQNLKDRFQLVQQEMQSFSSWMA 188
           V +  +++++ERLK+DK   + EL +  Q+ Q    Q+QN+  + Q+++Q  Q   S++A
Sbjct: 142 VGKFGIEEEVERLKRDKNVLMQELVRLRQQQQATENQLQNVGQKVQVMEQRQQQMMSFLA 201

Query: 189 RLLKKPGL--------RLDPLPQLETLERKRRLPRVSYNNSEDNV 224
           + ++ PG           D   Q+    +KRRLP     N  DNV
Sbjct: 202 KAVQSPGFLNQLVQQNNNDGNRQIPGSNKKRRLPVDEQENRGDNV 246

BLAST of Spg036573 vs. TAIR 10
Match: AT5G16820.2 (heat shock factor 3 )

HSP 1 Score: 194.1 bits (492), Expect = 2.3e-49
Identity = 107/225 (47.56%), Postives = 147/225 (65.33%), Query Frame = 0

Query: 9   LTSLPPFLVKTYDMVDDPSTNSIVSWSSSDKSFVVWNPPEFSSVLLPKFFKHSNFSSFIR 68
           + S+PPFL KTYDMVDDP TN +VSWSS + SFVVW+ PEFS VLLPK+FKH+NFSSF+R
Sbjct: 22  VNSVPPFLSKTYDMVDDPLTNEVVSWSSGNNSFVVWSAPEFSKVLLPKYFKHNNFSSFVR 81

Query: 69  QLNTYGFRKVDPEQWEFANEDFVKGQPHLMRNIHRRKPVH-SHSLQNLHGQGIS-PPLTE 128
           QLNTYGFRKVDP++WEFANE F++G+  L+++I RRKP H   + Q    Q  S     E
Sbjct: 82  QLNTYGFRKVDPDRWEFANEGFLRGRKQLLKSIVRRKPSHVQQNQQQTQVQSSSVGACVE 141

Query: 129 VERKSLKDDIERLKQDKEQHLLELRKNEQEYQEVNLQMQNLKDRFQLVQQEMQSFSSWMA 188
           V +  +++++ERLK+DK   + EL +  Q+ Q    Q+QN+  + Q+++Q  Q   S++A
Sbjct: 142 VGKFGIEEEVERLKRDKNVLMQELVRLRQQQQATENQLQNVGQKVQVMEQRQQQMMSFLA 201

Query: 189 RLLKKPGL--------RLDPLPQLETLERKRRLPRVSYNNSEDNV 224
           + ++ PG           D   Q+    +KRRLP     N  DNV
Sbjct: 202 KAVQSPGFLNQLVQQNNNDGNRQIPGSNKKRRLPVDEQENRGDNV 246

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6583940.14.7e-19377.11Heat stress transcription factor A-4a, partial [Cucurbita argyrosperma subsp. so... [more]
XP_022927503.19.8e-19186.55heat stress transcription factor A-4c-like [Cucurbita moschata] >XP_022927504.1 ... [more]
XP_011657567.12.2e-19087.06heat stress transcription factor A-4c [Cucumis sativus] >XP_031736076.1 heat str... [more]
XP_038895068.12.9e-19086.55heat stress transcription factor A-4a [Benincasa hispida] >XP_038895070.1 heat s... [more]
XP_023519250.11.8e-18985.53heat stress transcription factor A-4a-like [Cucurbita pepo subsp. pepo] >XP_0235... [more]
Match NameE-valueIdentityDescription
O494031.0e-8948.00Heat stress transcription factor A-4a OS=Arabidopsis thaliana OX=3702 GN=HSFA4A ... [more]
Q9FK721.2e-8247.85Heat stress transcription factor A-4c OS=Arabidopsis thaliana OX=3702 GN=HSFA4C ... [more]
Q94J168.6e-7342.06Heat stress transcription factor A-4b OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q93VB56.0e-5837.56Heat stress transcription factor A-4d OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q94BZ59.3e-5133.56Heat stress transcription factor A-5 OS=Arabidopsis thaliana OX=3702 GN=HSFA5 PE... [more]
Match NameE-valueIdentityDescription
A0A6J1EI674.7e-19186.55heat stress transcription factor A-4c-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A0A0LV221.1e-19087.06HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G467710 ... [more]
A0A6J1KEK17.6e-18987.11heat stress transcription factor A-4a-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A1S4DVL42.7e-18685.28heat stress transcription factor A-4c OS=Cucumis melo OX=3656 GN=LOC103487107 PE... [more]
A0A5A7UU402.9e-18085.90Heat stress transcription factor A-4c OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
Match NameE-valueIdentityDescription
AT4G18880.17.2e-9148.00heat shock transcription factor A4A [more]
AT5G45710.18.5e-8447.85winged-helix DNA-binding transcription factor family protein [more]
AT4G13980.16.6e-5233.56winged-helix DNA-binding transcription factor family protein [more]
AT5G16820.12.3e-4947.56heat shock factor 3 [more]
AT5G16820.22.3e-4947.56heat shock factor 3 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 129..177
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 355..378
NoneNo IPR availablePANTHERPTHR10015:SF161HEAT STRESS TRANSCRIPTION FACTOR A-4Ccoord: 1..392
IPR000232Heat shock factor (HSF)-type, DNA-bindingPRINTSPR00056HSFDOMAINcoord: 15..38
score: 46.07
coord: 66..78
score: 84.18
coord: 53..65
score: 72.09
IPR000232Heat shock factor (HSF)-type, DNA-bindingSMARTSM00415hsfneu3coord: 11..104
e-value: 2.2E-61
score: 219.9
IPR000232Heat shock factor (HSF)-type, DNA-bindingPFAMPF00447HSF_DNA-bindcoord: 15..104
e-value: 4.0E-31
score: 107.5
IPR000232Heat shock factor (HSF)-type, DNA-bindingPROSITEPS00434HSF_DOMAINcoord: 54..78
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 9..105
e-value: 3.6E-37
score: 129.0
IPR027725Heat shock transcription factor familyPANTHERPTHR10015HEAT SHOCK TRANSCRIPTION FACTORcoord: 1..392
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 11..104

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg036573.1Spg036573.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034605 cellular response to heat
biological_process GO:0006357 regulation of transcription by RNA polymerase II
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
molecular_function GO:0043565 sequence-specific DNA binding