Spg035871 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg035871 vs. NCBI nr
Match: XP_023541502.1 (uncharacterized protein LOC111801664 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023541503.1 uncharacterized protein LOC111801664 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023541504.1 uncharacterized protein LOC111801664 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023541505.1 uncharacterized protein LOC111801664 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023541506.1 uncharacterized protein LOC111801664 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023541507.1 uncharacterized protein LOC111801664 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2216.8 bits (5743), Expect = 0.0e+00
Identity = 1198/1478 (81.06%), Postives = 1293/1478 (87.48%), Query Frame = 0

Query: 1    MVEDVVPKLESSNSCCKAWKNEYIKLDEKRNALRQAIKFLEQQITKIQAEKLNLQKGYEE 60
            M++D VP  ES+NSCCK WK++   ++EKRNALRQA+K L+QQI +IQAE LNL+KGYEE
Sbjct: 1    MLDDFVPNPESANSCCKRWKDKCTVVEEKRNALRQAVKLLQQQINRIQAENLNLKKGYEE 60

Query: 61   EKARASIEREGKDKESAIRVSLEREILDLKSQISSLRQNDVNAVNVRGEVEHLNALVAEG 120
            EKA ASIEREGK+KESAIRVSLEREILDLKS ISSLRQNDV+AV V  EVE LNALVAEG
Sbjct: 61   EKAGASIEREGKEKESAIRVSLEREILDLKSHISSLRQNDVDAVKVCREVEQLNALVAEG 120

Query: 121  KKEICQLKELLGTEKRRTDVERKNAEARKEEAAQALKTVKIERSKASDLRKFHKTEMDKV 180
            KKEI  L ELL TEKR+TD ERKNAE RKEEAAQALKTV+IERSKA DLRK HKTE+DKV
Sbjct: 121  KKEISHLNELLETEKRKTDAERKNAEVRKEEAAQALKTVRIERSKACDLRKLHKTELDKV 180

Query: 181  NECRQQLEILKKEYEETKLKLASETSKLIEVKKDLEIEKKRTFKERERADSEMSKAEASR 240
             E RQQLE+LKKEYEETKLKLASETSKLIEVKKDLEIEK+RT KERERA+SEMSKA ASR
Sbjct: 181  KESRQQLEMLKKEYEETKLKLASETSKLIEVKKDLEIEKRRTSKERERANSEMSKAHASR 240

Query: 241  IQAEANRKQVEEEKSRAENLFQQLKRKTCKIEELQKQVKEHQTLKNFIESCCGQHDKKRD 300
            +QAEANRKQ EEE+S+AENLFQQL+RKTCKIEELQKQVKE QTLK FIESCCGQHD+K D
Sbjct: 241  VQAEANRKQAEEEQSKAENLFQQLERKTCKIEELQKQVKELQTLKTFIESCCGQHDEKTD 300

Query: 301  SKAVEKNDKPWLEMIQRNADELKLVFEFLKAKEVNIMHKMDGDLAIMKEKPVDSNMMKSS 360
             KAVEKNDK  LEMIQ+NA+ELKL FEF+K KEVNIMHKMDGDLAIMKEKPVDSN+MKSS
Sbjct: 301  GKAVEKNDKSLLEMIQKNANELKLAFEFMKDKEVNIMHKMDGDLAIMKEKPVDSNVMKSS 360

Query: 361  ELKNHLEIYRRKAMDEQCRADKLSLELEEKKRKVEELQKKLREFKSSRKLVDASSVSFEH 420
            ELK HLEIYR+KAMDEQCRADKL+LELEEKKRKVEELQK LRE KSSRKLVDAS+VSFEH
Sbjct: 361  ELKKHLEIYRKKAMDEQCRADKLALELEEKKRKVEELQKNLRELKSSRKLVDASAVSFEH 420

Query: 421  AMSSERAEMKLLKKKLKFEKTRLKHAREVANLEKTHRSIIQQELGRFKLEFVQLSNHLDN 480
            AMSSERAEMKLLKKKLKFEKTRLKHAREVANLE THRSIIQ ELGRFKL+FVQLSN+LDN
Sbjct: 421  AMSSERAEMKLLKKKLKFEKTRLKHAREVANLENTHRSIIQHELGRFKLKFVQLSNYLDN 480

Query: 481  LHKFASTGSKDNDDLEKTKNAENLQSLYSKKNLHAIEPFQTWMPDTFRRTTPQHGTPLFP 540
            LHKFASTG+K +DDLEKTKNAENL+SLY++KNLHAIEPF+TW+P+TFR+TTPQH  PL P
Sbjct: 481  LHKFASTGAKGSDDLEKTKNAENLRSLYAEKNLHAIEPFKTWLPETFRQTTPQHDAPLLP 540

Query: 541  LSGGNHITSLSGIESWLESHPGDSNRKMLQSCAVNSSTASFSDGQLVGSQEKAGICLTAA 600
            LSGGNH+TSLSGIES LE+HP +S+RKM QSCAVNSSTASFSDGQL GSQEKAG+CLTAA
Sbjct: 541  LSGGNHVTSLSGIESRLEAHPVNSDRKMFQSCAVNSSTASFSDGQLAGSQEKAGLCLTAA 600

Query: 601  KLVGENLNMQPKISNVSGEVSKMKSNENLAIMAENSVRSPIKNHVGRANEKQQKRKRTVE 660
            KLVGENL M+PKISNVSGEVS+MK  EN A MAENSVRSPIKNHVGRANEKQQKRKRT+E
Sbjct: 601  KLVGENLIMKPKISNVSGEVSEMKDIEN-ARMAENSVRSPIKNHVGRANEKQQKRKRTIE 660

Query: 661  TVESIEYLYHESKKMHSQIEEKLSLLHALNSPTEKPLE---------------------K 720
             VE+IE LYHES+K+HSQIEEKLSLLHALNSPTEKPLE                     K
Sbjct: 661  AVENIECLYHESRKIHSQIEEKLSLLHALNSPTEKPLEKSGHVISNVFQDPSADKKARKK 720

Query: 721  RKTSGRKKTRVQHLLDANEMKLNKVDTEVCTFQSIDRQPSQPFSKHTDSCQPCLEEPNNS 780
            RKT  RKKTR Q LLD NEM+L+KV+ EV   ++  RQPSQP SK TD+ QPC EE NNS
Sbjct: 721  RKTLCRKKTREQ-LLDDNEMELSKVNIEVRALENFGRQPSQPVSKLTDNLQPCSEELNNS 780

Query: 781  VISELQSLGTFGNIADGDYMKLLELDSAADEECYRRAMEMPLSPSLPDIYIPGAETSALN 840
             ISELQ+ GT GNI+DGDYMKLL+LDSAADEECYRRAMEMPLSPSLPDIYIPGAETS LN
Sbjct: 781  AISELQTPGTLGNISDGDYMKLLDLDSAADEECYRRAMEMPLSPSLPDIYIPGAETSTLN 840

Query: 841  DLEPLVDELHKELPNEEEGQPQLHGHDVMDVEIKSNCTQSCNSGLLGDSHGSKRQLG--L 900
            + EPL+DE H      EEGQPQLH +DVMDVEIKSN  + CNSGLLGD H SKR L    
Sbjct: 841  EFEPLLDERH------EEGQPQLHSYDVMDVEIKSNYPRYCNSGLLGDIHSSKRHLDPCF 900

Query: 901  WQGSRGSDLCDIVQAEKNCLDQIGVIVGMPGTNVPLSGCEGVGTSEIKSGTPDNSIPDFC 960
             QGS GSDLCDIV AE+NCLDQIG+ V  PGTNVPLSGCEGVG SE+KSGT DNSIPDFC
Sbjct: 901  MQGSHGSDLCDIVHAEENCLDQIGITVEKPGTNVPLSGCEGVGASEMKSGTLDNSIPDFC 960

Query: 961  VLFSYTKDCHSISRIFSATTACIKRSSLTSHKEWMVQEILASLNMEHKLLPKEKTCVFFS 1020
            VLFS TKDC SISRIFSAT AC KRSSLTS K WMVQEILASLNMEHKLLP EK CVFFS
Sbjct: 961  VLFSNTKDCRSISRIFSATRACSKRSSLTSKKGWMVQEILASLNMEHKLLPMEKACVFFS 1020

Query: 1021 LLVLNFTVVAGHKYGNFLNCNTCLDSFSGHICEAMLDVEIRSLFTELLCLDELLALIEDF 1080
            LL+LNF VVA HKYGNFLNCN+CLDSFSGHICEAMLDVEIRSLFTELLCLDELL+LIEDF
Sbjct: 1021 LLLLNFNVVAVHKYGNFLNCNSCLDSFSGHICEAMLDVEIRSLFTELLCLDELLSLIEDF 1080

Query: 1081 IIDGRVLSCTDASFETLTEGILRVNIPIDGVNRTLSLAPASTNYLIAGSSILASISKAVH 1140
            IIDGR+LSC DAS ET  EG+LRVNI +DGVNR LSL PAS NYLIAGSSILASISK VH
Sbjct: 1081 IIDGRILSCIDASLETSIEGVLRVNISVDGVNRALSLTPASRNYLIAGSSILASISKVVH 1140

Query: 1141 RTGLLWEVSYSILRNRRCESSLVLTMLHIFAHIGGDQFFSLKGYSTLRAVLKSIIIHLE- 1200
            RTG LWEVSYSILR  R ESSLVLTMLHIFAHIGGDQFFSL+GYSTL AVLKSII HLE 
Sbjct: 1141 RTGFLWEVSYSILRICRYESSLVLTMLHIFAHIGGDQFFSLEGYSTLMAVLKSIITHLEV 1200

Query: 1201 VRSSDDATFTPPKSNCRTEFVQCANCPFSDEVMSMPMVVSFLLQFIHKNISNEIMAGDLE 1260
            V SSDDA+FTPPK NCRTEFVQCA+CPFS   MSMPM VSFLL+ +HKN     +A DLE
Sbjct: 1201 VGSSDDASFTPPKGNCRTEFVQCAHCPFSANSMSMPMAVSFLLRLVHKN----TLAEDLE 1260

Query: 1261 NPTSSLNSDSLFEKNIANRIPSKNSSGKLVHPASYLDCDASCCLKKYKVSDDESHSLFNP 1320
            NPT SLN +SL EKNIAN+IP KN SG+ +HPA YLDCDASCCLKKY+VSDDES SLFNP
Sbjct: 1261 NPTGSLNPESLSEKNIANQIPCKNLSGQEIHPALYLDCDASCCLKKYRVSDDESWSLFNP 1320

Query: 1321 ILCDVTDAISLVELLACYMTWNWTFVNIISQLLELLKSSVKESLPIVILLGQLGRLGVAA 1380
             LC++TDAISLVELLACYM WNWTF NIISQLLE LKSSVKESLP VILLGQLGR GVAA
Sbjct: 1321 TLCEITDAISLVELLACYMGWNWTFANIISQLLEFLKSSVKESLPFVILLGQLGRFGVAA 1380

Query: 1381 GGFEDGGVKILRSNLAAFLCLDSTIKSGLRVQIAIVSALLGLLPYDFETIVQDRVSYPAS 1440
            GGFEDGGVKILRSNL+AFL LD+TIKSGL VQIAIVSALLGLLP+DFETI+QD+VSYPA 
Sbjct: 1381 GGFEDGGVKILRSNLSAFLYLDTTIKSGLCVQIAIVSALLGLLPFDFETIIQDKVSYPA- 1440

Query: 1441 SSSQYVEVKLIKTWFSLLSPKQKELSCNILQAAVCNVS 1455
            SS+QYVEV LIKTWFS LSPKQKELSCN LQ AVC+VS
Sbjct: 1441 SSNQYVEVNLIKTWFSFLSPKQKELSCNTLQVAVCSVS 1465

BLAST of Spg035871 vs. NCBI nr
Match: XP_022954739.1 (restin homolog isoform X1 [Cucurbita moschata] >XP_022954740.1 restin homolog isoform X1 [Cucurbita moschata] >XP_022954741.1 restin homolog isoform X1 [Cucurbita moschata] >XP_022954742.1 restin homolog isoform X1 [Cucurbita moschata] >XP_022954743.1 restin homolog isoform X1 [Cucurbita moschata])

HSP 1 Score: 2216.4 bits (5742), Expect = 0.0e+00
Identity = 1198/1478 (81.06%), Postives = 1293/1478 (87.48%), Query Frame = 0

Query: 1    MVEDVVPKLESSNSCCKAWKNEYIKLDEKRNALRQAIKFLEQQITKIQAEKLNLQKGYEE 60
            M++D VP  ES+NSCCK WK++  +++EKRNALRQA+K L+QQI KIQAE LNL+KGYEE
Sbjct: 1    MLDDFVPNPESANSCCKRWKDKCTEVEEKRNALRQAVKLLQQQINKIQAENLNLKKGYEE 60

Query: 61   EKARASIEREGKDKESAIRVSLEREILDLKSQISSLRQNDVNAVNVRGEVEHLNALVAEG 120
            +KA ASIEREGK+KESAIRVSLEREILDLKS ISSLRQNDV+AV V  EVE LNALVAEG
Sbjct: 61   DKAGASIEREGKEKESAIRVSLEREILDLKSHISSLRQNDVDAVEVCREVEQLNALVAEG 120

Query: 121  KKEICQLKELLGTEKRRTDVERKNAEARKEEAAQALKTVKIERSKASDLRKFHKTEMDKV 180
            KKEI  L ELL TEKR+TD ERKNAE RKEEAAQALKTV+IERSKASDLRK HK E+DKV
Sbjct: 121  KKEISHLNELLETEKRKTDAERKNAEVRKEEAAQALKTVRIERSKASDLRKLHKNELDKV 180

Query: 181  NECRQQLEILKKEYEETKLKLASETSKLIEVKKDLEIEKKRTFKERERADSEMSKAEASR 240
             ECRQQLE+LKKEYEETKLKLASETSKL EVKKDLEIEK+RT KERERA+SEMSKA  SR
Sbjct: 181  KECRQQLEMLKKEYEETKLKLASETSKLNEVKKDLEIEKRRTSKERERANSEMSKAHVSR 240

Query: 241  IQAEANRKQVEEEKSRAENLFQQLKRKTCKIEELQKQVKEHQTLKNFIESCCGQHDKKRD 300
            IQAEANRKQ EEE+S+AENL QQL RKTCKIEELQKQVKE QTLK FIESCCGQHD+K D
Sbjct: 241  IQAEANRKQAEEEQSKAENLLQQLDRKTCKIEELQKQVKELQTLKTFIESCCGQHDEKTD 300

Query: 301  SKAVEKNDKPWLEMIQRNADELKLVFEFLKAKEVNIMHKMDGDLAIMKEKPVDSNMMKSS 360
             KAVEKN KPWLE+IQ+NA+E KL FEFLK KEVNIMHKMDGDLAIMKEKP+DSNMMKSS
Sbjct: 301  GKAVEKNVKPWLEVIQKNANEFKLAFEFLKDKEVNIMHKMDGDLAIMKEKPLDSNMMKSS 360

Query: 361  ELKNHLEIYRRKAMDEQCRADKLSLELEEKKRKVEELQKKLREFKSSRKLVDASSVSFEH 420
            ELK HLEIYR+KAMDEQ RADKL+LELEEKKRKVE+LQK LRE KSSRKLVDAS+VSFEH
Sbjct: 361  ELKKHLEIYRKKAMDEQYRADKLALELEEKKRKVEKLQKNLRELKSSRKLVDASAVSFEH 420

Query: 421  AMSSERAEMKLLKKKLKFEKTRLKHAREVANLEKTHRSIIQQELGRFKLEFVQLSNHLDN 480
            AMSSERAEMKLLKKKLKFEKTRLKHAREVANLE THRSIIQ ELG FKL+FVQLSN+LDN
Sbjct: 421  AMSSERAEMKLLKKKLKFEKTRLKHAREVANLENTHRSIIQHELGSFKLKFVQLSNYLDN 480

Query: 481  LHKFASTGSKDNDDLEKTKNAENLQSLYSKKNLHAIEPFQTWMPDTFRRTTPQHGTPLFP 540
            LHKFASTG+K NDDLEKTKNAENL+SLY++KNLHAIEPF+TW+PDTFR+TTPQH  PL P
Sbjct: 481  LHKFASTGAKGNDDLEKTKNAENLRSLYAEKNLHAIEPFKTWLPDTFRQTTPQHDAPLLP 540

Query: 541  LSGGNHITSLSGIESWLESHPGDSNRKMLQSCAVNSSTASFSDGQLVGSQEKAGICLTAA 600
            LSGGNH+TSLSGIES LE+HP +S+RKM QSCAVNSSTASFSDGQL GS+EKAG+CLTAA
Sbjct: 541  LSGGNHVTSLSGIESRLEAHPVNSDRKMFQSCAVNSSTASFSDGQLAGSREKAGLCLTAA 600

Query: 601  KLVGENLNMQPKISNVSGEVSKMKSNENLAIMAENSVRSPIKNHVGRANEKQQKRKRTVE 660
            KLVGENL M+PKISNVSGEVS+MK NEN A MAENSVRSPIKNHVGRANEKQQKRKRT+E
Sbjct: 601  KLVGENLIMKPKISNVSGEVSEMKDNEN-ARMAENSVRSPIKNHVGRANEKQQKRKRTIE 660

Query: 661  TVESIEYLYHESKKMHSQIEEKLSLLHALNSPTEKPLE---------------------K 720
             VE+IE LYHES+K+HSQIEEKLSLLHALNSPTEKPL+                     K
Sbjct: 661  AVENIECLYHESRKIHSQIEEKLSLLHALNSPTEKPLDKSGHVISNVFQDPSADKKARKK 720

Query: 721  RKTSGRKKTRVQHLLDANEMKLNKVDTEVCTFQSIDRQPSQPFSKHTDSCQPCLEEPNNS 780
            RKT  +KKTR Q LLD NEM+L+KV+ EVC  +S  RQPSQP SK TD+ QPCLEE NNS
Sbjct: 721  RKTLCQKKTREQ-LLDDNEMELSKVNIEVCALESFGRQPSQPVSKLTDNLQPCLEELNNS 780

Query: 781  VISELQSLGTFGNIADGDYMKLLELDSAADEECYRRAMEMPLSPSLPDIYIPGAETSALN 840
             ISELQ+LGT GNI+DGDYMKLL+LDSAADEECYRRAMEMPLSPSLPDIYIPGAETS LN
Sbjct: 781  AISELQTLGTLGNISDGDYMKLLDLDSAADEECYRRAMEMPLSPSLPDIYIPGAETSTLN 840

Query: 841  DLEPLVDELHKELPNEEEGQPQLHGHDVMDVEIKSNCTQSCNSGLLGDSHGSKRQLG--L 900
            + EPL+DE H      EEGQPQLH +DVMDVEIKSN TQ CNSGLLGD H SK  L    
Sbjct: 841  EFEPLLDERH------EEGQPQLHSYDVMDVEIKSNHTQYCNSGLLGDIHSSKHHLDPCF 900

Query: 901  WQGSRGSDLCDIVQAEKNCLDQIGVIVGMPGTNVPLSGCEGVGTSEIKSGTPDNSIPDFC 960
             QGS GSDLCDIVQA++N L+QIGV V MPGTNVPLSGCEGVG SEIKSGT DNSIPDFC
Sbjct: 901  MQGSHGSDLCDIVQAKENYLNQIGVTVEMPGTNVPLSGCEGVGASEIKSGTLDNSIPDFC 960

Query: 961  VLFSYTKDCHSISRIFSATTACIKRSSLTSHKEWMVQEILASLNMEHKLLPKEKTCVFFS 1020
            VLFS  KDC SISRIFSAT AC KRSSLT+ KEWMVQEILASLNMEHKLLP EK CVFFS
Sbjct: 961  VLFSNIKDCRSISRIFSATRACSKRSSLTNKKEWMVQEILASLNMEHKLLPMEKACVFFS 1020

Query: 1021 LLVLNFTVVAGHKYGNFLNCNTCLDSFSGHICEAMLDVEIRSLFTELLCLDELLALIEDF 1080
            LL+LNF VVA HKYGNFLNCNTCLDSFSGHICEAMLDVEIRSLFTE LCLDELL+LIEDF
Sbjct: 1021 LLLLNFNVVAVHKYGNFLNCNTCLDSFSGHICEAMLDVEIRSLFTESLCLDELLSLIEDF 1080

Query: 1081 IIDGRVLSCTDASFETLTEGILRVNIPIDGVNRTLSLAPASTNYLIAGSSILASISKAVH 1140
            IIDGR+LSC DAS ET  EG+LRVNI +DGVNR LSL PASTNYLIAGSSILASISKAV 
Sbjct: 1081 IIDGRILSCIDASLETSIEGVLRVNISVDGVNRALSLTPASTNYLIAGSSILASISKAVD 1140

Query: 1141 RTGLLWEVSYSILRNRRCESSLVLTMLHIFAHIGGDQFFSLKGYSTLRAVLKSIIIHLE- 1200
            RTG LWEVSYSILR  R ESSLVLTMLHIFAHIGGDQFFSL+GYSTL AVLKSII HLE 
Sbjct: 1141 RTGFLWEVSYSILRICRYESSLVLTMLHIFAHIGGDQFFSLEGYSTLMAVLKSIITHLEV 1200

Query: 1201 VRSSDDATFTPPKSNCRTEFVQCANCPFSDEVMSMPMVVSFLLQFIHKNISNEIMAGDLE 1260
            V SSDDA+FTPPK NCRTEFVQCA+CPFS  +MSMPM VSFLL+ +HKN     +A DLE
Sbjct: 1201 VGSSDDASFTPPKGNCRTEFVQCAHCPFSANIMSMPMAVSFLLRLVHKN----ALAEDLE 1260

Query: 1261 NPTSSLNSDSLFEKNIANRIPSKNSSGKLVHPASYLDCDASCCLKKYKVSDDESHSLFNP 1320
            NPT SLN +SL EKNIA +IP KN SG+ VHPA YLDCDASCCLKKY+VSDDES SLFNP
Sbjct: 1261 NPTGSLNPESLSEKNIAYQIPCKNLSGQEVHPALYLDCDASCCLKKYRVSDDESWSLFNP 1320

Query: 1321 ILCDVTDAISLVELLACYMTWNWTFVNIISQLLELLKSSVKESLPIVILLGQLGRLGVAA 1380
             LC++TDAISLVELLACYM WNWTF NIISQLLE LKSSVKESLP VILLGQLGR GVAA
Sbjct: 1321 SLCEITDAISLVELLACYMGWNWTFANIISQLLEFLKSSVKESLPFVILLGQLGRFGVAA 1380

Query: 1381 GGFEDGGVKILRSNLAAFLCLDSTIKSGLRVQIAIVSALLGLLPYDFETIVQDRVSYPAS 1440
            GGFEDGGVKILRSNL+AFL LD+TIKSGL VQIAIVSALLGLLP++FETI+QD+VSYPA 
Sbjct: 1381 GGFEDGGVKILRSNLSAFLYLDTTIKSGLCVQIAIVSALLGLLPFEFETIIQDKVSYPA- 1440

Query: 1441 SSSQYVEVKLIKTWFSLLSPKQKELSCNILQAAVCNVS 1455
            SS+ YVEV LIKTWFS LSPKQKELSCN LQ AVC+VS
Sbjct: 1441 SSNPYVEVNLIKTWFSFLSPKQKELSCNTLQVAVCSVS 1465

BLAST of Spg035871 vs. NCBI nr
Match: XP_022994711.1 (protein MLP1-like [Cucurbita maxima] >XP_022994712.1 protein MLP1-like [Cucurbita maxima] >XP_022994713.1 protein MLP1-like [Cucurbita maxima] >XP_022994714.1 protein MLP1-like [Cucurbita maxima] >XP_022994715.1 protein MLP1-like [Cucurbita maxima])

HSP 1 Score: 2215.3 bits (5739), Expect = 0.0e+00
Identity = 1196/1478 (80.92%), Postives = 1287/1478 (87.08%), Query Frame = 0

Query: 1    MVEDVVPKLESSNSCCKAWKNEYIKLDEKRNALRQAIKFLEQQITKIQAEKLNLQKGYEE 60
            M++D VP  ES+NSCCK WK++  +++EKRNALRQA+K L+QQI +IQAE LNL+KGYEE
Sbjct: 1    MLDDFVPNPESANSCCKRWKDKCTEVEEKRNALRQAVKLLQQQINRIQAENLNLKKGYEE 60

Query: 61   EKARASIEREGKDKESAIRVSLEREILDLKSQISSLRQNDVNAVNVRGEVEHLNALVAEG 120
            EKA ASIEREGK+KESAIRVSLEREILDLKS ISSLRQNDV+AV V  EVEHLNALVAEG
Sbjct: 61   EKAGASIEREGKEKESAIRVSLEREILDLKSHISSLRQNDVDAVKVCREVEHLNALVAEG 120

Query: 121  KKEICQLKELLGTEKRRTDVERKNAEARKEEAAQALKTVKIERSKASDLRKFHKTEMDKV 180
            KKEI  L ELL TEKR+TD ERKNAE RKEEAAQ LK+V+IERSK SDLRK HKTE+DKV
Sbjct: 121  KKEISHLNELLETEKRKTDAERKNAEVRKEEAAQTLKSVRIERSKGSDLRKLHKTELDKV 180

Query: 181  NECRQQLEILKKEYEETKLKLASETSKLIEVKKDLEIEKKRTFKERERADSEMSKAEASR 240
             ECRQQLE+LKKEYEETKLKL SETSKLIEVKK LEIEK+RT KERERA+SEMSKA ASR
Sbjct: 181  KECRQQLEMLKKEYEETKLKLESETSKLIEVKKGLEIEKRRTSKERERANSEMSKAHASR 240

Query: 241  IQAEANRKQVEEEKSRAENLFQQLKRKTCKIEELQKQVKEHQTLKNFIESCCGQHDKKRD 300
            +QAEANRKQ EEE+S+AENLFQQL+RKTCKIEELQKQVKE QTLK FIESCCGQHD+K D
Sbjct: 241  VQAEANRKQAEEEQSKAENLFQQLERKTCKIEELQKQVKELQTLKTFIESCCGQHDEKTD 300

Query: 301  SKAVEKNDKPWLEMIQRNADELKLVFEFLKAKEVNIMHKMDGDLAIMKEKPVDSNMMKSS 360
             KAVEKN KPWLE+IQ+NA+ELKL FEFLK KEVNIMHKMDGDL IMKEKPVDSN+MKSS
Sbjct: 301  GKAVEKNVKPWLEVIQKNANELKLAFEFLKDKEVNIMHKMDGDLVIMKEKPVDSNIMKSS 360

Query: 361  ELKNHLEIYRRKAMDEQCRADKLSLELEEKKRKVEELQKKLREFKSSRKLVDASSVSFEH 420
            ELK HLEIYR+KAMDEQCRADKL+LELEEKKRKVE+LQK LRE KSSRKLVDAS+VSFEH
Sbjct: 361  ELKKHLEIYRKKAMDEQCRADKLALELEEKKRKVEKLQKNLRELKSSRKLVDASAVSFEH 420

Query: 421  AMSSERAEMKLLKKKLKFEKTRLKHAREVANLEKTHRSIIQQELGRFKLEFVQLSNHLDN 480
            AMSSERAEMKLLKKKLKFEKTRLKHAREVANLE TH SIIQ ELGRFKL+FVQLSN+LDN
Sbjct: 421  AMSSERAEMKLLKKKLKFEKTRLKHAREVANLENTHHSIIQHELGRFKLKFVQLSNYLDN 480

Query: 481  LHKFASTGSKDNDDLEKTKNAENLQSLYSKKNLHAIEPFQTWMPDTFRRTTPQHGTPLFP 540
            LHKFASTG+K NDDLEKTKNAENL+SLY+ KNLHAIEPF+TW+PDTFR+TTPQH  PL P
Sbjct: 481  LHKFASTGAKGNDDLEKTKNAENLRSLYADKNLHAIEPFKTWLPDTFRQTTPQHDAPLLP 540

Query: 541  LSGGNHITSLSGIESWLESHPGDSNRKMLQSCAVNSSTASFSDGQLVGSQEKAGICLTAA 600
            LSGGNH+TSLSGIES LE+HP +S+RKM QSCAVNSSTASFSDGQL GSQEKAG+CLTAA
Sbjct: 541  LSGGNHVTSLSGIESRLEAHPVNSDRKMFQSCAVNSSTASFSDGQLAGSQEKAGLCLTAA 600

Query: 601  KLVGENLNMQPKISNVSGEVSKMKSNENLAIMAENSVRSPIKNHVGRANEKQQKRKRTVE 660
            KLVGENL M+PKISNVSGEVS+MK NE +A MAENSVRSPIKNHVGRANEKQQKRKRT+E
Sbjct: 601  KLVGENLIMKPKISNVSGEVSEMKDNE-IARMAENSVRSPIKNHVGRANEKQQKRKRTIE 660

Query: 661  TVESIEYLYHESKKMHSQIEEKLSLLHALNSPTEKPLE---------------------K 720
             VE+IE LYHES+K+HSQIEEKLSLLHALNSPTEKPLE                     K
Sbjct: 661  AVENIECLYHESRKIHSQIEEKLSLLHALNSPTEKPLEKRGHVISNVFQDPSADKKARKK 720

Query: 721  RKTSGRKKTRVQHLLDANEMKLNKVDTEVCTFQSIDRQPSQPFSKHTDSCQPCLEEPNNS 780
            RKT   KKTR Q LLD NEM+  +V+ EV   +S  RQPSQP SK TD+ QPC EE NNS
Sbjct: 721  RKTLCPKKTREQ-LLDDNEME-TQVNIEVRALESFGRQPSQPVSKLTDNLQPCSEELNNS 780

Query: 781  VISELQSLGTFGNIADGDYMKLLELDSAADEECYRRAMEMPLSPSLPDIYIPGAETSALN 840
             ISELQ+LGT GNI+DGDYMKLL+LDSAADEECYRRAMEMPLSPSLPDIYIPGAETS LN
Sbjct: 781  AISELQTLGTLGNISDGDYMKLLDLDSAADEECYRRAMEMPLSPSLPDIYIPGAETSTLN 840

Query: 841  DLEPLVDELHKELPNEEEGQPQLHGHDVMDVEIKSNCTQSCNSGLLGDSHGSKRQLG--L 900
            + EPL+DE H      EEGQPQLH +DVMDVEIKSN TQ CNSGLLGD H SKR L    
Sbjct: 841  EFEPLLDERH------EEGQPQLHSYDVMDVEIKSNYTQHCNSGLLGDIHSSKRHLDPCF 900

Query: 901  WQGSRGSDLCDIVQAEKNCLDQIGVIVGMPGTNVPLSGCEGVGTSEIKSGTPDNSIPDFC 960
             QG  GSDLCDIV AE+NCLDQIG+ V  PGTNVPLSGCEGVG S IKSGT DNSIPDFC
Sbjct: 901  MQGRHGSDLCDIVHAEENCLDQIGITVEKPGTNVPLSGCEGVGASAIKSGTLDNSIPDFC 960

Query: 961  VLFSYTKDCHSISRIFSATTACIKRSSLTSHKEWMVQEILASLNMEHKLLPKEKTCVFFS 1020
            VLFS TKDC SISRIFSAT AC KRSSLTS KEWMVQEILASLNMEHKLLP EK CVFFS
Sbjct: 961  VLFSNTKDCRSISRIFSATKACSKRSSLTSKKEWMVQEILASLNMEHKLLPMEKACVFFS 1020

Query: 1021 LLVLNFTVVAGHKYGNFLNCNTCLDSFSGHICEAMLDVEIRSLFTELLCLDELLALIEDF 1080
            LL+LNF VVA HKYGNFLNCNT LDSFSGHICEAMLDVEIRSLFTELLCLDELLALIEDF
Sbjct: 1021 LLLLNFNVVAVHKYGNFLNCNTYLDSFSGHICEAMLDVEIRSLFTELLCLDELLALIEDF 1080

Query: 1081 IIDGRVLSCTDASFETLTEGILRVNIPIDGVNRTLSLAPASTNYLIAGSSILASISKAVH 1140
            IIDGR+LSC DAS ET  EG+LRVNI +DGVNR LSL PASTNYLIAGSSILASISKAVH
Sbjct: 1081 IIDGRILSCIDASLETSIEGVLRVNISVDGVNRALSLTPASTNYLIAGSSILASISKAVH 1140

Query: 1141 RTGLLWEVSYSILRNRRCESSLVLTMLHIFAHIGGDQFFSLKGYSTLRAVLKSIIIHLE- 1200
            RTG LWEVSYSILR  R ESSLVLTMLHIFAHIGGDQFFSL+GYSTL AVLKSII HLE 
Sbjct: 1141 RTGFLWEVSYSILRICRYESSLVLTMLHIFAHIGGDQFFSLEGYSTLMAVLKSIITHLEV 1200

Query: 1201 VRSSDDATFTPPKSNCRTEFVQCANCPFSDEVMSMPMVVSFLLQFIHKNISNEIMAGDLE 1260
            V SSDDA+FTPPK NCRTEFVQCA CPFS  +MSMPM VSFLL+ IHKN     +A DLE
Sbjct: 1201 VGSSDDASFTPPKGNCRTEFVQCAYCPFSANIMSMPMAVSFLLRLIHKN----ALAEDLE 1260

Query: 1261 NPTSSLNSDSLFEKNIANRIPSKNSSGKLVHPASYLDCDASCCLKKYKVSDDESHSLFNP 1320
            NPT S+N +SL EKNIAN+IP +N SG+ VHPA YLDCDASCCLKKY+VSDD+S SLFNP
Sbjct: 1261 NPTGSINPESLSEKNIANQIPCENLSGQEVHPALYLDCDASCCLKKYRVSDDDSWSLFNP 1320

Query: 1321 ILCDVTDAISLVELLACYMTWNWTFVNIISQLLELLKSSVKESLPIVILLGQLGRLGVAA 1380
             LCD+TDAISLVELLACYM WNWTF NIISQLLE LKSSVKESLP VILLGQLGR GVA 
Sbjct: 1321 TLCDITDAISLVELLACYMGWNWTFANIISQLLEFLKSSVKESLPFVILLGQLGRFGVAT 1380

Query: 1381 GGFEDGGVKILRSNLAAFLCLDSTIKSGLRVQIAIVSALLGLLPYDFETIVQDRVSYPAS 1440
            GGFEDGGVKILRSNL+AFLCLD+TIKSGL VQIA+VSALLGLLP+DFETI+QD V YPA 
Sbjct: 1381 GGFEDGGVKILRSNLSAFLCLDTTIKSGLCVQIAVVSALLGLLPFDFETIIQDEVGYPA- 1440

Query: 1441 SSSQYVEVKLIKTWFSLLSPKQKELSCNILQAAVCNVS 1455
            S +QY EV LIKTWFS LSPKQKELSCN LQ AVC+VS
Sbjct: 1441 SLNQYGEVNLIKTWFSFLSPKQKELSCNTLQVAVCSVS 1464

BLAST of Spg035871 vs. NCBI nr
Match: XP_023519446.1 (uncharacterized protein LOC111782859 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023519447.1 uncharacterized protein LOC111782859 isoform X2 [Cucurbita pepo subsp. pepo] >XP_023519448.1 uncharacterized protein LOC111782859 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023519449.1 uncharacterized protein LOC111782859 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023519450.1 uncharacterized protein LOC111782859 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023519451.1 uncharacterized protein LOC111782859 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2205.3 bits (5713), Expect = 0.0e+00
Identity = 1186/1478 (80.24%), Postives = 1290/1478 (87.28%), Query Frame = 0

Query: 1    MVEDVVPKLESSNSCCKAWKNEYIKLDEKRNALRQAIKFLEQQITKIQAEKLNLQKGYEE 60
            MV DVV K ESSNSCCK WK+ Y KL+EKR ALRQA+K LE+QI KIQAE LNL++GYE+
Sbjct: 1    MVGDVVSKPESSNSCCKVWKDMYTKLEEKRIALRQAVKLLEEQIRKIQAENLNLKEGYEK 60

Query: 61   EKARASIEREGKDKESAIRVSLEREILDLKSQISSLRQNDVNAVNVRGEVEHLNALVAEG 120
            EKARAS+ERE KDKESAIRVSLEREI DLKSQISSLRQNDV AVNVRGEV+HLN LVAEG
Sbjct: 61   EKARASVERESKDKESAIRVSLEREISDLKSQISSLRQNDVGAVNVRGEVDHLNVLVAEG 120

Query: 121  KKEICQLKELLGTEKRRTDVERKNAEARKEEAAQALKTVKIERSKASDLRKFHKTEMDKV 180
            KK+I QLKELL TEKRRTD ERKNAEARKEEAAQALKT+KIERSKASDL+K HKTEMDKV
Sbjct: 121  KKKISQLKELLETEKRRTDAERKNAEARKEEAAQALKTMKIERSKASDLKKLHKTEMDKV 180

Query: 181  NECRQQLEILKKEYEETKLKLASETSKLIEVKKDLEIEKKRTFKERERADSEMSKAEASR 240
            NECRQQL +L+KEYEET LKLASETSKL EV KDLEIEK+RTFKE++RADSEMSKA+A R
Sbjct: 181  NECRQQLGVLEKEYEETNLKLASETSKLTEVMKDLEIEKQRTFKEKKRADSEMSKAQALR 240

Query: 241  IQAEANRKQVEEEKSRAENLFQQLKRKTCKIEELQKQVKEHQTLKNFIESCCGQHDKKRD 300
            +Q E   KQV EEKSRAENLFQQ +RKTCKI++LQKQVKE +TLK FIESCCGQ  K+ +
Sbjct: 241  MQTEVTMKQVGEEKSRAENLFQQSERKTCKIKKLQKQVKELKTLKKFIESCCGQPVKRTN 300

Query: 301  SKAVEKNDKPWLEMIQRNADELKLVFEFLKAKEVNIMHKMDGDLAIMKEKPVDSNMMKSS 360
            SK V+KNDKPWLEMIQRN +ELKL FEF+KAKEVNI HKMD DLAIMKEK V SNMMKSS
Sbjct: 301  SKDVKKNDKPWLEMIQRNENELKLAFEFVKAKEVNIKHKMDEDLAIMKEKTVSSNMMKSS 360

Query: 361  ELKNHLEIYRRKAMDEQCRADKLSLELEEKKRKVEELQKKLREFKSSRKLVDASSVSFEH 420
            ELKNHLEIYRRKAMDEQCRADKLSLELEEK RK+EELQK LREFKSSRKL DAS+VSFEH
Sbjct: 361  ELKNHLEIYRRKAMDEQCRADKLSLELEEKNRKIEELQKNLREFKSSRKLADASTVSFEH 420

Query: 421  AMSSERAEMKLLKKKLKFEKTRLKHAREVANLEKTHRSIIQQELGRFKLEFVQLSNHLDN 480
            AMSSERAEMKLLKKKLKFEKTRLKHAR+VANLEK HRS+IQQELGRFKLEFVQLSNHLD+
Sbjct: 421  AMSSERAEMKLLKKKLKFEKTRLKHARQVANLEKNHRSVIQQELGRFKLEFVQLSNHLDD 480

Query: 481  LHKFASTGSKDNDDLEKTKNAENLQSLYSKKNLHAIEPFQTWMPDTFRRTTPQHGTPLFP 540
            LHKF+STG+KDNDD EKT NAE LQS YSKKNL AIE FQ WMPDTFR+ TP HG PL P
Sbjct: 481  LHKFSSTGTKDNDDSEKTMNAEKLQSSYSKKNLRAIEAFQAWMPDTFRQATPHHGAPLLP 540

Query: 541  LSGGNHITSLSGIESWLESHPGDSNRKMLQSCAVNSSTASFSDGQLVGSQEKAGICLTAA 600
             S GNHITSLSGIES LES PGDSNRKMLQSCAVNSSTASFSDGQLVGSQEKAG+ LTA 
Sbjct: 541  SSVGNHITSLSGIESRLESFPGDSNRKMLQSCAVNSSTASFSDGQLVGSQEKAGLRLTAT 600

Query: 601  KLVGENLNMQPKISNVSGEVSKMKSNENLAIMAENSVRSPIKNHVGRANEKQQKRKRTVE 660
            KL GEN NMQP+ISN+S EV KMKSNENLA+MA NSVRS IKN+VGRANEKQ KRKRT+E
Sbjct: 601  KLAGENFNMQPRISNLSSEVGKMKSNENLAMMAGNSVRSHIKNNVGRANEKQGKRKRTIE 660

Query: 661  TVESIEYLYHESKKMHSQIEEKLSLLHALNSPTEKPLE---------------------K 720
            TVESI+YLYHESKKMHSQIEEKLSLLHALNSP EKPL+                     K
Sbjct: 661  TVESIDYLYHESKKMHSQIEEKLSLLHALNSPAEKPLDKSEHVISNVLQDSCADKKIRKK 720

Query: 721  RKTSGRKKTRVQHLLDANEMKLNKVDTEVCTFQSIDRQPSQPFSKHTDSCQPCLEEPNNS 780
            RK   +KK +VQHLLD +EMKLNKVDTEVC  +SI  +PSQP SK  D+CQPC+EE N  
Sbjct: 721  RKALCQKKLKVQHLLDNSEMKLNKVDTEVCAPKSIGIKPSQPVSKLMDNCQPCVEELNTR 780

Query: 781  VISELQSLGTFGNIADGDYMKLLELDSAADEECYRRAMEMPLSPSLPDIYIPGAETSALN 840
            VISELQSL TFGNIA+ DYMKLL+LDSAADEECYRRA+EMPLSPSLP+IYI GAETSALN
Sbjct: 781  VISELQSLETFGNIANVDYMKLLDLDSAADEECYRRAIEMPLSPSLPNIYISGAETSALN 840

Query: 841  DLEPLVDELHKELPNEEEGQPQLHGHDVMDVEIKSNCTQSCNSGLLGDSHGSKRQLG--L 900
            + EPLVDELHKELP+E EGQP+ H ++V+DVEIKSN TQSC   LLGD H SKRQL   L
Sbjct: 841  EFEPLVDELHKELPDEREGQPKTHSYNVIDVEIKSNYTQSCEFDLLGDIHSSKRQLDPCL 900

Query: 901  WQGSRGSDLCDIVQAEKNCLDQIGVIVGMPGTNVPLSGCEGVGTSEIKSGTPDNSIPDFC 960
             QG + +DL D+VQA  NCLDQ+GVIVGMPGTNV LSGCEGVG SEIKSGT  NS PDFC
Sbjct: 901  IQGRQENDLFDVVQAGNNCLDQVGVIVGMPGTNVSLSGCEGVGASEIKSGTLGNSNPDFC 960

Query: 961  VLFSYTKDCHSISRIFSATTACIKRSSLTSHKEWMVQEILASLNMEHKLLPKEKTCVFFS 1020
            VLFS +KDCHSI +IFSAT AC+KRSS+ + KEWMVQEILASLNMEH+L+PKEKTCVFFS
Sbjct: 961  VLFSNSKDCHSILKIFSATRACVKRSSIITQKEWMVQEILASLNMEHELVPKEKTCVFFS 1020

Query: 1021 LLVLNFTVVAGHKYGNFLNCNTCLDSFSGHICEAMLDVEIRSLFTELLCLDELLALIEDF 1080
            LL+LNFTVVA HKYGNFLNC+TCLDSFSGHICEAMLDV IRSLFT+LLCLD LLAL+EDF
Sbjct: 1021 LLLLNFTVVAVHKYGNFLNCHTCLDSFSGHICEAMLDVAIRSLFTKLLCLDALLALMEDF 1080

Query: 1081 IIDGRVLSCTDASFETLTEGILRVNIPIDGVNRTLSLAPASTNYLIAGSSILASISKAVH 1140
            +IDGRVLSCTDASFETLT+G+LRVNIPID VNRTLSL PAST+YLIAGSSILASISKAVH
Sbjct: 1081 LIDGRVLSCTDASFETLTQGVLRVNIPIDSVNRTLSLTPASTDYLIAGSSILASISKAVH 1140

Query: 1141 RTGLLWEVSYSILRNRRCESSLVLTMLHIFAHIGGDQFFSLKGYSTLRAVLKSIIIHLE- 1200
            RTGLLWE+SY ILR+ R ESSL+LT+LHIFAHIGGD FFSL+ YS LRAVLKSII HLE 
Sbjct: 1141 RTGLLWEISYRILRSCRYESSLMLTILHIFAHIGGDHFFSLEVYSNLRAVLKSIITHLET 1200

Query: 1201 VRSSDDATFTPPKSNCRTEFVQCANCPFSDEVMSMPMVVSFLLQFIHKNISNEIMAGDLE 1260
            V SS+DATFTP K NCR EFVQCANCPFS+E MSMPMVVSFLL+ + KNISNEIM  DLE
Sbjct: 1201 VGSSNDATFTPLKRNCRAEFVQCANCPFSEEGMSMPMVVSFLLRLLQKNISNEIMDEDLE 1260

Query: 1261 NPTSSLNSDSLFEKNIANRIPSKNSSGKLVHPASYLDCDASCCLKKYKVSDDESHSLFNP 1320
            NPTSSLN +SLF++N+AN+IP KNSSGK VHP+ YLDCDASCCLKK+KVSDDE   LFNP
Sbjct: 1261 NPTSSLNLESLFKRNLANQIPCKNSSGKEVHPSVYLDCDASCCLKKFKVSDDEPRFLFNP 1320

Query: 1321 ILCDVTDAISLVELLACYMTWNWTFVNIISQLLELLKSSVKESLPIVILLGQLGRLGVAA 1380
             LCDVTDAISLVELLA YM WNWTF NII QL+ELLKSSVK+   IVILLGQLGR GV A
Sbjct: 1321 TLCDVTDAISLVELLAWYMGWNWTFANIIPQLMELLKSSVKKGFAIVILLGQLGRFGVDA 1380

Query: 1381 GGFEDGGVKILRSNLAAFLCLDSTIKSGLRVQIAIVSALLGLLPYDFETIVQDRVSYPAS 1440
            GGFEDGGVKILRSNL++FLCLD+TIKSGL VQIA VS+LLGLLP+DFETIVQD+V Y A 
Sbjct: 1381 GGFEDGGVKILRSNLSSFLCLDTTIKSGLPVQIATVSSLLGLLPFDFETIVQDKVRYRA- 1440

Query: 1441 SSSQYVEVKLIKTWFSLLSPKQKELSCNILQAAVCNVS 1455
            SS QY EV LIK WFSLLSPKQKELSCNILQ A CNVS
Sbjct: 1441 SSYQYAEVNLIKMWFSLLSPKQKELSCNILQVAACNVS 1477

BLAST of Spg035871 vs. NCBI nr
Match: KAG6573415.1 (hypothetical protein SDJN03_27302, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 2201.8 bits (5704), Expect = 0.0e+00
Identity = 1190/1476 (80.62%), Postives = 1288/1476 (87.26%), Query Frame = 0

Query: 1    MVEDVVPKLESSNSCCKAWKNEYIKLDEKRNALRQAIKFLEQQITKIQAEKLNLQKGYEE 60
            M++D VP  ES+NSCCK WK++  +++EKRNALRQA+K L+QQI KIQAE LNL+KGYEE
Sbjct: 1    MLDDFVPNPESANSCCKRWKDKCTEVEEKRNALRQAVKLLQQQINKIQAENLNLKKGYEE 60

Query: 61   EKARASIEREGKDKESAIRVSLEREILDLKSQISSLRQNDVNAVNVRGEVEHLNALVAEG 120
            +KA ASIEREGK+KESAIRVSLEREILDLKS ISSLRQNDV+AV V  EVE LNALVAEG
Sbjct: 61   DKAGASIEREGKEKESAIRVSLEREILDLKSHISSLRQNDVDAVEVCREVEQLNALVAEG 120

Query: 121  KKEICQLKELLGTEKRRTDVERKNAEARKEEAAQALKTVKIERSKASDLRKFHKTEMDKV 180
            KKEI  L ELL TEKR+TD ERKNAE RKEEAAQALKTV+IERSKASDL K HK E+DKV
Sbjct: 121  KKEISHLNELLETEKRKTDAERKNAEVRKEEAAQALKTVRIERSKASDLWKLHKNELDKV 180

Query: 181  NECRQQLEILKKEYEETKLKLASETSKLIEVKKDLEIEKKRTFKERERADSEMSKAEASR 240
             ECRQQLE+LKKEYEETKLKLASETSKL EVKKDLEIEK+RT KERERA+SEMSKA  SR
Sbjct: 181  KECRQQLEMLKKEYEETKLKLASETSKLNEVKKDLEIEKRRTSKERERANSEMSKAHVSR 240

Query: 241  IQAEANRKQVEEEKSRAENLFQQLKRKTCKIEELQKQVKEHQTLKNFIESCCGQHDKKRD 300
            IQAEANRKQ EEE+S+AENL QQL RKTCKIEELQKQVKE QTLK FIESCCGQHD+K D
Sbjct: 241  IQAEANRKQAEEEQSKAENLLQQLDRKTCKIEELQKQVKELQTLKTFIESCCGQHDEKTD 300

Query: 301  SKAVEKNDKPWLEMIQRNADELKLVFEFLKAKEVNIMHKMDGDLAIMKEKPVDSNMMKSS 360
             KAVEKN KPWLE+IQ+NA+E KL FEFLK KEVNIMHKMDGDLAIMKEKP+DSNMMKSS
Sbjct: 301  GKAVEKNVKPWLEVIQKNANEFKLAFEFLKDKEVNIMHKMDGDLAIMKEKPLDSNMMKSS 360

Query: 361  ELKNHLEIYRRKAMDEQCRADKLSLELEEKKRKVEELQKKLREFKSSRKLVDASSVSFEH 420
            ELK HLEIYR+KAMDEQ RADKL+LELEEKKRK E+LQK LRE KSSRKLVDAS+VSFEH
Sbjct: 361  ELKKHLEIYRKKAMDEQYRADKLALELEEKKRKFEKLQKNLRELKSSRKLVDASAVSFEH 420

Query: 421  AMSSERAEMKLLKKKLKFEKTRLKHAREVANLEKTHRSIIQQELGRFKLEFVQLSNHLDN 480
            AMSSERAEMKLLKKKLKFEKTRLKHAREVANLE THRSIIQ ELG FKL+FVQLSN+LDN
Sbjct: 421  AMSSERAEMKLLKKKLKFEKTRLKHAREVANLENTHRSIIQHELGSFKLKFVQLSNYLDN 480

Query: 481  LHKFASTGSKDNDDLEKTKNAENLQSLYSKKNLHAIEPFQTWMPDTFRRTTPQHGTPLFP 540
            LHKFASTG+K NDDLEKTKNAENL+SLY++KNLHAIEPF+TW+PDTFR+TTPQH  PL P
Sbjct: 481  LHKFASTGAKGNDDLEKTKNAENLRSLYAEKNLHAIEPFKTWLPDTFRQTTPQHDAPLLP 540

Query: 541  LSGGNHITSLSGIESWLESHPGDSNRKMLQSCAVNSSTASFSDGQLVGSQEKAGICLTAA 600
            LSGGNH+TSLSGIES LE+HP +S+RKM QSCAVNSSTASFSDGQL GS+EKAG+CLTAA
Sbjct: 541  LSGGNHVTSLSGIESRLEAHPVNSDRKMFQSCAVNSSTASFSDGQLAGSREKAGLCLTAA 600

Query: 601  KLVGENLNMQPKISNVSGEVSKMKSNENLAIMAENSVRSPIKNHVGRANEKQQKRKRTVE 660
            KLVGENL M+PKISNV GEVS+MK NEN A MAENSVRSPIKNHVGRANEKQQKRKRT+E
Sbjct: 601  KLVGENLIMKPKISNVFGEVSEMKDNEN-ARMAENSVRSPIKNHVGRANEKQQKRKRTIE 660

Query: 661  TVESIEYLYHESKKMHSQIEEKLSLLHALNSPTEKPLE---------------------K 720
             VE+IE LYHES+K+HSQIEEKLSLLHALNSPTEKPL+                     K
Sbjct: 661  AVENIECLYHESRKIHSQIEEKLSLLHALNSPTEKPLDKSGHVISNVFQDPSADKKARKK 720

Query: 721  RKTSGRKKTRVQHLLDANEMKLNKVDTEVCTFQSIDRQPSQPFSKHTDSCQPCLEEPNNS 780
            RKT  +KKTR Q LLD NEM+L+KV+ EVC  +S  RQPSQP SK TD+ QPCLEE NNS
Sbjct: 721  RKTLCQKKTREQ-LLDDNEMELSKVNIEVCALESFGRQPSQPVSKLTDNLQPCLEELNNS 780

Query: 781  VISELQSLGTFGNIADGDYMKLLELDSAADEECYRRAMEMPLSPSLPDIYIPGAETSALN 840
             ISELQ+LGT GNI+DGDYMKLL+LDSAADEECYRRAMEMPLSPSLPDIYIPGAETS LN
Sbjct: 781  AISELQTLGTLGNISDGDYMKLLDLDSAADEECYRRAMEMPLSPSLPDIYIPGAETSTLN 840

Query: 841  DLEPLVDELHKELPNEEEGQPQLHGHDVMDVEIKSNCTQSCNSGLLGDSHGSKRQLG--L 900
            + EPL+DE H      EEGQPQLH +DVMDVEIKSN TQ CNSGLLGD H SK  L    
Sbjct: 841  EFEPLLDERH------EEGQPQLHSYDVMDVEIKSNHTQYCNSGLLGDIHSSKHHLDPCF 900

Query: 901  WQGSRGSDLCDIVQAEKNCLDQIGVIVGMPGTNVPLSGCEGVGTSEIKSGTPDNSIPDFC 960
             QGS GSDLCDIVQA++N L+QIGV V +PGTNVPLSGCEGVG SEIKSGT DNSIPDFC
Sbjct: 901  MQGSHGSDLCDIVQAKENYLNQIGVTVELPGTNVPLSGCEGVGASEIKSGTLDNSIPDFC 960

Query: 961  VLFSYTKDCHSISRIFSATTACIKRSSLTSHKEWMVQEILASLNMEHKLLPKEKTCVFFS 1020
            VLFS  KDC SISRIFSAT AC KRSSLT+ KEWMV+EILASLNMEHKLLP EK CVFFS
Sbjct: 961  VLFSNIKDCRSISRIFSATRACSKRSSLTNKKEWMVREILASLNMEHKLLPMEKACVFFS 1020

Query: 1021 LLVLNFTVVAGHKYGNFLNCNTCLDSFSGHICEAMLDVEIRSLFTELLCLDELLALIEDF 1080
            LL+LNF VVA HKYGNFLNCNTCLDSFSGHICEAMLDVEIRSLFTE LCLDELL+LIEDF
Sbjct: 1021 LLLLNFNVVAVHKYGNFLNCNTCLDSFSGHICEAMLDVEIRSLFTESLCLDELLSLIEDF 1080

Query: 1081 IIDGRVLSCTDASFETLTEGILRVNIPIDGVNRTLSLAPASTNYLIAGSSILASISKAVH 1140
            IIDGR+LSC DAS ET  EG+LRV+I +DGVNR LSL PASTNYLIAGSSILASISKAV 
Sbjct: 1081 IIDGRILSCIDASLETSIEGVLRVDISVDGVNRALSLTPASTNYLIAGSSILASISKAVD 1140

Query: 1141 RTGLLWEVSYSILRNRRCESSLVLTMLHIFAHIGGDQFFSLKGYSTLRAVLKSIIIHLE- 1200
            RTG LWEVSYSILR  R ESSLVLTMLHIFAHIGGDQFFSL+GYSTL AVLKSII HLE 
Sbjct: 1141 RTGFLWEVSYSILRICRYESSLVLTMLHIFAHIGGDQFFSLEGYSTLMAVLKSIITHLEV 1200

Query: 1201 VRSSDDATFTPPKSNCRTEFVQCANCPFSDEVMSMPMVVSFLLQFIHKNISNEIMAGDLE 1260
            V SSDDA+FTPPK NCRTEFVQCA+CPFS  +MSMPM VSFLL+ +HKN     +A DLE
Sbjct: 1201 VGSSDDASFTPPKGNCRTEFVQCAHCPFSANIMSMPMAVSFLLRLVHKN----ALAEDLE 1260

Query: 1261 NPTSSLNSDSLFEKNIANRIPSKNSSGKLVHPASYLDCDASCCLKKYKVSDDESHSLFNP 1320
            NPT SLN +SL EKNIA +IP KN SG+ VHPA YLDCDASCCLKKY+VSDDES SLFNP
Sbjct: 1261 NPTGSLNPESLSEKNIAYQIPCKNLSGQEVHPALYLDCDASCCLKKYRVSDDESWSLFNP 1320

Query: 1321 ILCDVTDAISLVELLACYMTWNWTFVNIISQLLELLKSSVKESLPIVILLGQLGRLGVAA 1380
             LC++TDAISLVELLACYM WNWTF NIISQLLE LKSSVKESLP VILLGQLGR GVAA
Sbjct: 1321 SLCEITDAISLVELLACYMGWNWTFANIISQLLEFLKSSVKESLPFVILLGQLGRFGVAA 1380

Query: 1381 GGFEDGGVKILRSNLAAFLCLDSTIKSGLRVQIAIVSALLGLLPYDFETIVQDRVSYPAS 1440
            GGFEDGGVKILRSNL+AFL LD+TIKSGL VQIAIVSALLGLLP++FETI+QD+VSYPA 
Sbjct: 1381 GGFEDGGVKILRSNLSAFLYLDTTIKSGLCVQIAIVSALLGLLPFEFETIIQDKVSYPA- 1440

Query: 1441 SSSQYVEVKLIKTWFSLLSPKQKELSCNILQAAVCN 1453
            SS+ YVEV LIKTWFS LSPKQKELSCN LQ AVC+
Sbjct: 1441 SSNPYVEVNLIKTWFSFLSPKQKELSCNTLQVAVCS 1463

BLAST of Spg035871 vs. ExPASy TrEMBL
Match: A0A6J1GRR8 (restin homolog isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456908 PE=4 SV=1)

HSP 1 Score: 2216.4 bits (5742), Expect = 0.0e+00
Identity = 1198/1478 (81.06%), Postives = 1293/1478 (87.48%), Query Frame = 0

Query: 1    MVEDVVPKLESSNSCCKAWKNEYIKLDEKRNALRQAIKFLEQQITKIQAEKLNLQKGYEE 60
            M++D VP  ES+NSCCK WK++  +++EKRNALRQA+K L+QQI KIQAE LNL+KGYEE
Sbjct: 1    MLDDFVPNPESANSCCKRWKDKCTEVEEKRNALRQAVKLLQQQINKIQAENLNLKKGYEE 60

Query: 61   EKARASIEREGKDKESAIRVSLEREILDLKSQISSLRQNDVNAVNVRGEVEHLNALVAEG 120
            +KA ASIEREGK+KESAIRVSLEREILDLKS ISSLRQNDV+AV V  EVE LNALVAEG
Sbjct: 61   DKAGASIEREGKEKESAIRVSLEREILDLKSHISSLRQNDVDAVEVCREVEQLNALVAEG 120

Query: 121  KKEICQLKELLGTEKRRTDVERKNAEARKEEAAQALKTVKIERSKASDLRKFHKTEMDKV 180
            KKEI  L ELL TEKR+TD ERKNAE RKEEAAQALKTV+IERSKASDLRK HK E+DKV
Sbjct: 121  KKEISHLNELLETEKRKTDAERKNAEVRKEEAAQALKTVRIERSKASDLRKLHKNELDKV 180

Query: 181  NECRQQLEILKKEYEETKLKLASETSKLIEVKKDLEIEKKRTFKERERADSEMSKAEASR 240
             ECRQQLE+LKKEYEETKLKLASETSKL EVKKDLEIEK+RT KERERA+SEMSKA  SR
Sbjct: 181  KECRQQLEMLKKEYEETKLKLASETSKLNEVKKDLEIEKRRTSKERERANSEMSKAHVSR 240

Query: 241  IQAEANRKQVEEEKSRAENLFQQLKRKTCKIEELQKQVKEHQTLKNFIESCCGQHDKKRD 300
            IQAEANRKQ EEE+S+AENL QQL RKTCKIEELQKQVKE QTLK FIESCCGQHD+K D
Sbjct: 241  IQAEANRKQAEEEQSKAENLLQQLDRKTCKIEELQKQVKELQTLKTFIESCCGQHDEKTD 300

Query: 301  SKAVEKNDKPWLEMIQRNADELKLVFEFLKAKEVNIMHKMDGDLAIMKEKPVDSNMMKSS 360
             KAVEKN KPWLE+IQ+NA+E KL FEFLK KEVNIMHKMDGDLAIMKEKP+DSNMMKSS
Sbjct: 301  GKAVEKNVKPWLEVIQKNANEFKLAFEFLKDKEVNIMHKMDGDLAIMKEKPLDSNMMKSS 360

Query: 361  ELKNHLEIYRRKAMDEQCRADKLSLELEEKKRKVEELQKKLREFKSSRKLVDASSVSFEH 420
            ELK HLEIYR+KAMDEQ RADKL+LELEEKKRKVE+LQK LRE KSSRKLVDAS+VSFEH
Sbjct: 361  ELKKHLEIYRKKAMDEQYRADKLALELEEKKRKVEKLQKNLRELKSSRKLVDASAVSFEH 420

Query: 421  AMSSERAEMKLLKKKLKFEKTRLKHAREVANLEKTHRSIIQQELGRFKLEFVQLSNHLDN 480
            AMSSERAEMKLLKKKLKFEKTRLKHAREVANLE THRSIIQ ELG FKL+FVQLSN+LDN
Sbjct: 421  AMSSERAEMKLLKKKLKFEKTRLKHAREVANLENTHRSIIQHELGSFKLKFVQLSNYLDN 480

Query: 481  LHKFASTGSKDNDDLEKTKNAENLQSLYSKKNLHAIEPFQTWMPDTFRRTTPQHGTPLFP 540
            LHKFASTG+K NDDLEKTKNAENL+SLY++KNLHAIEPF+TW+PDTFR+TTPQH  PL P
Sbjct: 481  LHKFASTGAKGNDDLEKTKNAENLRSLYAEKNLHAIEPFKTWLPDTFRQTTPQHDAPLLP 540

Query: 541  LSGGNHITSLSGIESWLESHPGDSNRKMLQSCAVNSSTASFSDGQLVGSQEKAGICLTAA 600
            LSGGNH+TSLSGIES LE+HP +S+RKM QSCAVNSSTASFSDGQL GS+EKAG+CLTAA
Sbjct: 541  LSGGNHVTSLSGIESRLEAHPVNSDRKMFQSCAVNSSTASFSDGQLAGSREKAGLCLTAA 600

Query: 601  KLVGENLNMQPKISNVSGEVSKMKSNENLAIMAENSVRSPIKNHVGRANEKQQKRKRTVE 660
            KLVGENL M+PKISNVSGEVS+MK NEN A MAENSVRSPIKNHVGRANEKQQKRKRT+E
Sbjct: 601  KLVGENLIMKPKISNVSGEVSEMKDNEN-ARMAENSVRSPIKNHVGRANEKQQKRKRTIE 660

Query: 661  TVESIEYLYHESKKMHSQIEEKLSLLHALNSPTEKPLE---------------------K 720
             VE+IE LYHES+K+HSQIEEKLSLLHALNSPTEKPL+                     K
Sbjct: 661  AVENIECLYHESRKIHSQIEEKLSLLHALNSPTEKPLDKSGHVISNVFQDPSADKKARKK 720

Query: 721  RKTSGRKKTRVQHLLDANEMKLNKVDTEVCTFQSIDRQPSQPFSKHTDSCQPCLEEPNNS 780
            RKT  +KKTR Q LLD NEM+L+KV+ EVC  +S  RQPSQP SK TD+ QPCLEE NNS
Sbjct: 721  RKTLCQKKTREQ-LLDDNEMELSKVNIEVCALESFGRQPSQPVSKLTDNLQPCLEELNNS 780

Query: 781  VISELQSLGTFGNIADGDYMKLLELDSAADEECYRRAMEMPLSPSLPDIYIPGAETSALN 840
             ISELQ+LGT GNI+DGDYMKLL+LDSAADEECYRRAMEMPLSPSLPDIYIPGAETS LN
Sbjct: 781  AISELQTLGTLGNISDGDYMKLLDLDSAADEECYRRAMEMPLSPSLPDIYIPGAETSTLN 840

Query: 841  DLEPLVDELHKELPNEEEGQPQLHGHDVMDVEIKSNCTQSCNSGLLGDSHGSKRQLG--L 900
            + EPL+DE H      EEGQPQLH +DVMDVEIKSN TQ CNSGLLGD H SK  L    
Sbjct: 841  EFEPLLDERH------EEGQPQLHSYDVMDVEIKSNHTQYCNSGLLGDIHSSKHHLDPCF 900

Query: 901  WQGSRGSDLCDIVQAEKNCLDQIGVIVGMPGTNVPLSGCEGVGTSEIKSGTPDNSIPDFC 960
             QGS GSDLCDIVQA++N L+QIGV V MPGTNVPLSGCEGVG SEIKSGT DNSIPDFC
Sbjct: 901  MQGSHGSDLCDIVQAKENYLNQIGVTVEMPGTNVPLSGCEGVGASEIKSGTLDNSIPDFC 960

Query: 961  VLFSYTKDCHSISRIFSATTACIKRSSLTSHKEWMVQEILASLNMEHKLLPKEKTCVFFS 1020
            VLFS  KDC SISRIFSAT AC KRSSLT+ KEWMVQEILASLNMEHKLLP EK CVFFS
Sbjct: 961  VLFSNIKDCRSISRIFSATRACSKRSSLTNKKEWMVQEILASLNMEHKLLPMEKACVFFS 1020

Query: 1021 LLVLNFTVVAGHKYGNFLNCNTCLDSFSGHICEAMLDVEIRSLFTELLCLDELLALIEDF 1080
            LL+LNF VVA HKYGNFLNCNTCLDSFSGHICEAMLDVEIRSLFTE LCLDELL+LIEDF
Sbjct: 1021 LLLLNFNVVAVHKYGNFLNCNTCLDSFSGHICEAMLDVEIRSLFTESLCLDELLSLIEDF 1080

Query: 1081 IIDGRVLSCTDASFETLTEGILRVNIPIDGVNRTLSLAPASTNYLIAGSSILASISKAVH 1140
            IIDGR+LSC DAS ET  EG+LRVNI +DGVNR LSL PASTNYLIAGSSILASISKAV 
Sbjct: 1081 IIDGRILSCIDASLETSIEGVLRVNISVDGVNRALSLTPASTNYLIAGSSILASISKAVD 1140

Query: 1141 RTGLLWEVSYSILRNRRCESSLVLTMLHIFAHIGGDQFFSLKGYSTLRAVLKSIIIHLE- 1200
            RTG LWEVSYSILR  R ESSLVLTMLHIFAHIGGDQFFSL+GYSTL AVLKSII HLE 
Sbjct: 1141 RTGFLWEVSYSILRICRYESSLVLTMLHIFAHIGGDQFFSLEGYSTLMAVLKSIITHLEV 1200

Query: 1201 VRSSDDATFTPPKSNCRTEFVQCANCPFSDEVMSMPMVVSFLLQFIHKNISNEIMAGDLE 1260
            V SSDDA+FTPPK NCRTEFVQCA+CPFS  +MSMPM VSFLL+ +HKN     +A DLE
Sbjct: 1201 VGSSDDASFTPPKGNCRTEFVQCAHCPFSANIMSMPMAVSFLLRLVHKN----ALAEDLE 1260

Query: 1261 NPTSSLNSDSLFEKNIANRIPSKNSSGKLVHPASYLDCDASCCLKKYKVSDDESHSLFNP 1320
            NPT SLN +SL EKNIA +IP KN SG+ VHPA YLDCDASCCLKKY+VSDDES SLFNP
Sbjct: 1261 NPTGSLNPESLSEKNIAYQIPCKNLSGQEVHPALYLDCDASCCLKKYRVSDDESWSLFNP 1320

Query: 1321 ILCDVTDAISLVELLACYMTWNWTFVNIISQLLELLKSSVKESLPIVILLGQLGRLGVAA 1380
             LC++TDAISLVELLACYM WNWTF NIISQLLE LKSSVKESLP VILLGQLGR GVAA
Sbjct: 1321 SLCEITDAISLVELLACYMGWNWTFANIISQLLEFLKSSVKESLPFVILLGQLGRFGVAA 1380

Query: 1381 GGFEDGGVKILRSNLAAFLCLDSTIKSGLRVQIAIVSALLGLLPYDFETIVQDRVSYPAS 1440
            GGFEDGGVKILRSNL+AFL LD+TIKSGL VQIAIVSALLGLLP++FETI+QD+VSYPA 
Sbjct: 1381 GGFEDGGVKILRSNLSAFLYLDTTIKSGLCVQIAIVSALLGLLPFEFETIIQDKVSYPA- 1440

Query: 1441 SSSQYVEVKLIKTWFSLLSPKQKELSCNILQAAVCNVS 1455
            SS+ YVEV LIKTWFS LSPKQKELSCN LQ AVC+VS
Sbjct: 1441 SSNPYVEVNLIKTWFSFLSPKQKELSCNTLQVAVCSVS 1465

BLAST of Spg035871 vs. ExPASy TrEMBL
Match: A0A6J1JWM7 (protein MLP1-like OS=Cucurbita maxima OX=3661 GN=LOC111490360 PE=4 SV=1)

HSP 1 Score: 2215.3 bits (5739), Expect = 0.0e+00
Identity = 1196/1478 (80.92%), Postives = 1287/1478 (87.08%), Query Frame = 0

Query: 1    MVEDVVPKLESSNSCCKAWKNEYIKLDEKRNALRQAIKFLEQQITKIQAEKLNLQKGYEE 60
            M++D VP  ES+NSCCK WK++  +++EKRNALRQA+K L+QQI +IQAE LNL+KGYEE
Sbjct: 1    MLDDFVPNPESANSCCKRWKDKCTEVEEKRNALRQAVKLLQQQINRIQAENLNLKKGYEE 60

Query: 61   EKARASIEREGKDKESAIRVSLEREILDLKSQISSLRQNDVNAVNVRGEVEHLNALVAEG 120
            EKA ASIEREGK+KESAIRVSLEREILDLKS ISSLRQNDV+AV V  EVEHLNALVAEG
Sbjct: 61   EKAGASIEREGKEKESAIRVSLEREILDLKSHISSLRQNDVDAVKVCREVEHLNALVAEG 120

Query: 121  KKEICQLKELLGTEKRRTDVERKNAEARKEEAAQALKTVKIERSKASDLRKFHKTEMDKV 180
            KKEI  L ELL TEKR+TD ERKNAE RKEEAAQ LK+V+IERSK SDLRK HKTE+DKV
Sbjct: 121  KKEISHLNELLETEKRKTDAERKNAEVRKEEAAQTLKSVRIERSKGSDLRKLHKTELDKV 180

Query: 181  NECRQQLEILKKEYEETKLKLASETSKLIEVKKDLEIEKKRTFKERERADSEMSKAEASR 240
             ECRQQLE+LKKEYEETKLKL SETSKLIEVKK LEIEK+RT KERERA+SEMSKA ASR
Sbjct: 181  KECRQQLEMLKKEYEETKLKLESETSKLIEVKKGLEIEKRRTSKERERANSEMSKAHASR 240

Query: 241  IQAEANRKQVEEEKSRAENLFQQLKRKTCKIEELQKQVKEHQTLKNFIESCCGQHDKKRD 300
            +QAEANRKQ EEE+S+AENLFQQL+RKTCKIEELQKQVKE QTLK FIESCCGQHD+K D
Sbjct: 241  VQAEANRKQAEEEQSKAENLFQQLERKTCKIEELQKQVKELQTLKTFIESCCGQHDEKTD 300

Query: 301  SKAVEKNDKPWLEMIQRNADELKLVFEFLKAKEVNIMHKMDGDLAIMKEKPVDSNMMKSS 360
             KAVEKN KPWLE+IQ+NA+ELKL FEFLK KEVNIMHKMDGDL IMKEKPVDSN+MKSS
Sbjct: 301  GKAVEKNVKPWLEVIQKNANELKLAFEFLKDKEVNIMHKMDGDLVIMKEKPVDSNIMKSS 360

Query: 361  ELKNHLEIYRRKAMDEQCRADKLSLELEEKKRKVEELQKKLREFKSSRKLVDASSVSFEH 420
            ELK HLEIYR+KAMDEQCRADKL+LELEEKKRKVE+LQK LRE KSSRKLVDAS+VSFEH
Sbjct: 361  ELKKHLEIYRKKAMDEQCRADKLALELEEKKRKVEKLQKNLRELKSSRKLVDASAVSFEH 420

Query: 421  AMSSERAEMKLLKKKLKFEKTRLKHAREVANLEKTHRSIIQQELGRFKLEFVQLSNHLDN 480
            AMSSERAEMKLLKKKLKFEKTRLKHAREVANLE TH SIIQ ELGRFKL+FVQLSN+LDN
Sbjct: 421  AMSSERAEMKLLKKKLKFEKTRLKHAREVANLENTHHSIIQHELGRFKLKFVQLSNYLDN 480

Query: 481  LHKFASTGSKDNDDLEKTKNAENLQSLYSKKNLHAIEPFQTWMPDTFRRTTPQHGTPLFP 540
            LHKFASTG+K NDDLEKTKNAENL+SLY+ KNLHAIEPF+TW+PDTFR+TTPQH  PL P
Sbjct: 481  LHKFASTGAKGNDDLEKTKNAENLRSLYADKNLHAIEPFKTWLPDTFRQTTPQHDAPLLP 540

Query: 541  LSGGNHITSLSGIESWLESHPGDSNRKMLQSCAVNSSTASFSDGQLVGSQEKAGICLTAA 600
            LSGGNH+TSLSGIES LE+HP +S+RKM QSCAVNSSTASFSDGQL GSQEKAG+CLTAA
Sbjct: 541  LSGGNHVTSLSGIESRLEAHPVNSDRKMFQSCAVNSSTASFSDGQLAGSQEKAGLCLTAA 600

Query: 601  KLVGENLNMQPKISNVSGEVSKMKSNENLAIMAENSVRSPIKNHVGRANEKQQKRKRTVE 660
            KLVGENL M+PKISNVSGEVS+MK NE +A MAENSVRSPIKNHVGRANEKQQKRKRT+E
Sbjct: 601  KLVGENLIMKPKISNVSGEVSEMKDNE-IARMAENSVRSPIKNHVGRANEKQQKRKRTIE 660

Query: 661  TVESIEYLYHESKKMHSQIEEKLSLLHALNSPTEKPLE---------------------K 720
             VE+IE LYHES+K+HSQIEEKLSLLHALNSPTEKPLE                     K
Sbjct: 661  AVENIECLYHESRKIHSQIEEKLSLLHALNSPTEKPLEKRGHVISNVFQDPSADKKARKK 720

Query: 721  RKTSGRKKTRVQHLLDANEMKLNKVDTEVCTFQSIDRQPSQPFSKHTDSCQPCLEEPNNS 780
            RKT   KKTR Q LLD NEM+  +V+ EV   +S  RQPSQP SK TD+ QPC EE NNS
Sbjct: 721  RKTLCPKKTREQ-LLDDNEME-TQVNIEVRALESFGRQPSQPVSKLTDNLQPCSEELNNS 780

Query: 781  VISELQSLGTFGNIADGDYMKLLELDSAADEECYRRAMEMPLSPSLPDIYIPGAETSALN 840
             ISELQ+LGT GNI+DGDYMKLL+LDSAADEECYRRAMEMPLSPSLPDIYIPGAETS LN
Sbjct: 781  AISELQTLGTLGNISDGDYMKLLDLDSAADEECYRRAMEMPLSPSLPDIYIPGAETSTLN 840

Query: 841  DLEPLVDELHKELPNEEEGQPQLHGHDVMDVEIKSNCTQSCNSGLLGDSHGSKRQLG--L 900
            + EPL+DE H      EEGQPQLH +DVMDVEIKSN TQ CNSGLLGD H SKR L    
Sbjct: 841  EFEPLLDERH------EEGQPQLHSYDVMDVEIKSNYTQHCNSGLLGDIHSSKRHLDPCF 900

Query: 901  WQGSRGSDLCDIVQAEKNCLDQIGVIVGMPGTNVPLSGCEGVGTSEIKSGTPDNSIPDFC 960
             QG  GSDLCDIV AE+NCLDQIG+ V  PGTNVPLSGCEGVG S IKSGT DNSIPDFC
Sbjct: 901  MQGRHGSDLCDIVHAEENCLDQIGITVEKPGTNVPLSGCEGVGASAIKSGTLDNSIPDFC 960

Query: 961  VLFSYTKDCHSISRIFSATTACIKRSSLTSHKEWMVQEILASLNMEHKLLPKEKTCVFFS 1020
            VLFS TKDC SISRIFSAT AC KRSSLTS KEWMVQEILASLNMEHKLLP EK CVFFS
Sbjct: 961  VLFSNTKDCRSISRIFSATKACSKRSSLTSKKEWMVQEILASLNMEHKLLPMEKACVFFS 1020

Query: 1021 LLVLNFTVVAGHKYGNFLNCNTCLDSFSGHICEAMLDVEIRSLFTELLCLDELLALIEDF 1080
            LL+LNF VVA HKYGNFLNCNT LDSFSGHICEAMLDVEIRSLFTELLCLDELLALIEDF
Sbjct: 1021 LLLLNFNVVAVHKYGNFLNCNTYLDSFSGHICEAMLDVEIRSLFTELLCLDELLALIEDF 1080

Query: 1081 IIDGRVLSCTDASFETLTEGILRVNIPIDGVNRTLSLAPASTNYLIAGSSILASISKAVH 1140
            IIDGR+LSC DAS ET  EG+LRVNI +DGVNR LSL PASTNYLIAGSSILASISKAVH
Sbjct: 1081 IIDGRILSCIDASLETSIEGVLRVNISVDGVNRALSLTPASTNYLIAGSSILASISKAVH 1140

Query: 1141 RTGLLWEVSYSILRNRRCESSLVLTMLHIFAHIGGDQFFSLKGYSTLRAVLKSIIIHLE- 1200
            RTG LWEVSYSILR  R ESSLVLTMLHIFAHIGGDQFFSL+GYSTL AVLKSII HLE 
Sbjct: 1141 RTGFLWEVSYSILRICRYESSLVLTMLHIFAHIGGDQFFSLEGYSTLMAVLKSIITHLEV 1200

Query: 1201 VRSSDDATFTPPKSNCRTEFVQCANCPFSDEVMSMPMVVSFLLQFIHKNISNEIMAGDLE 1260
            V SSDDA+FTPPK NCRTEFVQCA CPFS  +MSMPM VSFLL+ IHKN     +A DLE
Sbjct: 1201 VGSSDDASFTPPKGNCRTEFVQCAYCPFSANIMSMPMAVSFLLRLIHKN----ALAEDLE 1260

Query: 1261 NPTSSLNSDSLFEKNIANRIPSKNSSGKLVHPASYLDCDASCCLKKYKVSDDESHSLFNP 1320
            NPT S+N +SL EKNIAN+IP +N SG+ VHPA YLDCDASCCLKKY+VSDD+S SLFNP
Sbjct: 1261 NPTGSINPESLSEKNIANQIPCENLSGQEVHPALYLDCDASCCLKKYRVSDDDSWSLFNP 1320

Query: 1321 ILCDVTDAISLVELLACYMTWNWTFVNIISQLLELLKSSVKESLPIVILLGQLGRLGVAA 1380
             LCD+TDAISLVELLACYM WNWTF NIISQLLE LKSSVKESLP VILLGQLGR GVA 
Sbjct: 1321 TLCDITDAISLVELLACYMGWNWTFANIISQLLEFLKSSVKESLPFVILLGQLGRFGVAT 1380

Query: 1381 GGFEDGGVKILRSNLAAFLCLDSTIKSGLRVQIAIVSALLGLLPYDFETIVQDRVSYPAS 1440
            GGFEDGGVKILRSNL+AFLCLD+TIKSGL VQIA+VSALLGLLP+DFETI+QD V YPA 
Sbjct: 1381 GGFEDGGVKILRSNLSAFLCLDTTIKSGLCVQIAVVSALLGLLPFDFETIIQDEVGYPA- 1440

Query: 1441 SSSQYVEVKLIKTWFSLLSPKQKELSCNILQAAVCNVS 1455
            S +QY EV LIKTWFS LSPKQKELSCN LQ AVC+VS
Sbjct: 1441 SLNQYGEVNLIKTWFSFLSPKQKELSCNTLQVAVCSVS 1464

BLAST of Spg035871 vs. ExPASy TrEMBL
Match: A0A6J1EFZ6 (myosin heavy chain, non-muscle-like OS=Cucurbita moschata OX=3662 GN=LOC111433978 PE=4 SV=1)

HSP 1 Score: 2182.9 bits (5655), Expect = 0.0e+00
Identity = 1178/1478 (79.70%), Postives = 1288/1478 (87.14%), Query Frame = 0

Query: 1    MVEDVVPKLESSNSCCKAWKNEYIKLDEKRNALRQAIKFLEQQITKIQAEKLNLQKGYEE 60
            MV DVV K ESSNSCCK WK+ Y KL+EKR ALRQA+K LE+QI KIQAE LNL++GYE+
Sbjct: 1    MVGDVVSKPESSNSCCKVWKDMYTKLEEKRIALRQAVKLLEEQIRKIQAENLNLKEGYEK 60

Query: 61   EKARASIEREGKDKESAIRVSLEREILDLKSQISSLRQNDVNAVNVRGEVEHLNALVAEG 120
            EKARASIERE KDKESAIRVSLEREI DLKSQISSLRQNDV AVNVRGEV+HLN LVAEG
Sbjct: 61   EKARASIERESKDKESAIRVSLEREISDLKSQISSLRQNDVEAVNVRGEVDHLNVLVAEG 120

Query: 121  KKEICQLKELLGTEKRRTDVERKNAEARKEEAAQALKTVKIERSKASDLRKFHKTEMDKV 180
            KK+I QLKELL TEKRRTD ERKNAEARKEEAAQALKT+KIERSKASDL+K HKTEMDKV
Sbjct: 121  KKKISQLKELLETEKRRTDAERKNAEARKEEAAQALKTMKIERSKASDLKKLHKTEMDKV 180

Query: 181  NECRQQLEILKKEYEETKLKLASETSKLIEVKKDLEIEKKRTFKERERADSEMSKAEASR 240
            NECRQQL +L+KEYEETKLKLASETSKL EV KDLEIEK+RTFKE++RADSEMSKA+ASR
Sbjct: 181  NECRQQLGMLEKEYEETKLKLASETSKLTEVMKDLEIEKQRTFKEKKRADSEMSKAQASR 240

Query: 241  IQAEANRKQVEEEKSRAENLFQQLKRKTCKIEELQKQVKEHQTLKNFIESCCGQHDKKRD 300
            +Q E   KQV EEKSRAENLFQQL+RKTCKI++L+KQVKE +TLK FIESCCGQ  K+ +
Sbjct: 241  MQTEVTVKQVGEEKSRAENLFQQLERKTCKIKKLRKQVKELKTLKKFIESCCGQPVKRTN 300

Query: 301  SKAVEKNDKPWLEMIQRNADELKLVFEFLKAKEVNIMHKMDGDLAIMKEKPVDSNMMKSS 360
            SK V+KNDKPWLEMIQRN +ELKL FE +KAKEVNI +KMD DLAIMKEK V+SNMMK+S
Sbjct: 301  SKDVKKNDKPWLEMIQRNENELKLAFECVKAKEVNINYKMDEDLAIMKEKTVNSNMMKAS 360

Query: 361  ELKNHLEIYRRKAMDEQCRADKLSLELEEKKRKVEELQKKLREFKSSRKLVDASSVSFEH 420
            ELKNHLEIYRRKAMDEQCRADKLSLELEEK RK+EELQK LREFKSSRKL DAS+VSFEH
Sbjct: 361  ELKNHLEIYRRKAMDEQCRADKLSLELEEKNRKIEELQKNLREFKSSRKLADASAVSFEH 420

Query: 421  AMSSERAEMKLLKKKLKFEKTRLKHAREVANLEKTHRSIIQQELGRFKLEFVQLSNHLDN 480
            AMSSERAEMKLLKKKLKFEKTRLKHAR+VANLEK HRS+IQQELGRFKLEFVQLSNHLD+
Sbjct: 421  AMSSERAEMKLLKKKLKFEKTRLKHARQVANLEKNHRSVIQQELGRFKLEFVQLSNHLDD 480

Query: 481  LHKFASTGSKDNDDLEKTKNAENLQSLYSKKNLHAIEPFQTWMPDTFRRTTPQHGTPLFP 540
            LHKF+STG+KDNDD EKT NAE LQS YSKKNL AIE FQ WMPD FR+ TP HG PL P
Sbjct: 481  LHKFSSTGTKDNDDSEKTMNAEKLQSSYSKKNLRAIEAFQAWMPDNFRQATPHHGAPLLP 540

Query: 541  LSGGNHITSLSGIESWLESHPGDSNRKMLQSCAVNSSTASFSDGQLVGSQEKAGICLTAA 600
             S GNHITSLSGIES LES PGDSNRKMLQSCAVNSSTASFSDGQLVGSQEKAG+ LTA 
Sbjct: 541  SSVGNHITSLSGIESRLESFPGDSNRKMLQSCAVNSSTASFSDGQLVGSQEKAGLRLTAT 600

Query: 601  KLVGENLNMQPKISNVSGEVSKMKSNENLAIMAENSVRSPIKNHVGRANEKQQKRKRTVE 660
            KL GEN NMQP+ISN+S EVSKMKSNENLA+MA NSVRS IKN VGRANEKQ KRKRT+E
Sbjct: 601  KLAGENFNMQPRISNLSSEVSKMKSNENLAMMAGNSVRSHIKNSVGRANEKQGKRKRTIE 660

Query: 661  TVESIEYLYHESKKMHSQIEEKLSLLHALNSPTEKPLE---------------------K 720
            TVESI+YLYHESKKMHSQIEEKLSLLHALNSPTEK L+                     K
Sbjct: 661  TVESIDYLYHESKKMHSQIEEKLSLLHALNSPTEKALDKSEHVISNVLQDSCADKKIRKK 720

Query: 721  RKTSGRKKTRVQHLLDANEMKLNKVDTEVCTFQSIDRQPSQPFSKHTDSCQPCLEEPNNS 780
            RK   +KK +VQHLLD +EMKLNKVDTEVC  +SI  +PSQP SK  D+CQPC+EE N  
Sbjct: 721  RKALCQKKLKVQHLLDNSEMKLNKVDTEVCAPKSIGIKPSQPVSKLMDNCQPCVEELNTH 780

Query: 781  VISELQSLGTFGNIADGDYMKLLELDSAADEECYRRAMEMPLSPSLPDIYIPGAETSALN 840
            VISELQSL TFGNIA+ DYMKLL+LDSAADEECYRRA+EMPLSPSLP+IYI GAETSA N
Sbjct: 781  VISELQSLETFGNIANVDYMKLLDLDSAADEECYRRAIEMPLSPSLPNIYISGAETSASN 840

Query: 841  DLEPLVDELHKELPNEEEGQPQLHGHDVMDVEIKSNCTQSCNSGLLGDSHGSKRQLG--L 900
            + EPLVDELHKELP+E EGQP+ H ++V+DVEIKSN TQSC+  LL D H SK QL   L
Sbjct: 841  EFEPLVDELHKELPDEREGQPKTHSYNVIDVEIKSNYTQSCDFDLLADIHSSKCQLDPCL 900

Query: 901  WQGSRGSDLCDIVQAEKNCLDQIGVIVGMPGTNVPLSGCEGVGTSEIKSGTPDNSIPDFC 960
             QG + +DL D+VQA  NCLDQ+GVIVGMPGTNV LSGCE VG SEIKSGT  NS PDFC
Sbjct: 901  IQGRQENDLFDVVQAGNNCLDQVGVIVGMPGTNVSLSGCEEVGASEIKSGTLGNSNPDFC 960

Query: 961  VLFSYTKDCHSISRIFSATTACIKRSSLTSHKEWMVQEILASLNMEHKLLPKEKTCVFFS 1020
            VLFS +KDCHSI +IFSAT AC+KRSS+ + KEWMVQEILASLNMEH+L+PKEKTCVFFS
Sbjct: 961  VLFSNSKDCHSILKIFSATRACVKRSSIITQKEWMVQEILASLNMEHELVPKEKTCVFFS 1020

Query: 1021 LLVLNFTVVAGHKYGNFLNCNTCLDSFSGHICEAMLDVEIRSLFTELLCLDELLALIEDF 1080
            LL+LNFTVVA HKYGNFLNC+TCLDSFSGHICEAMLDV IRSLFT+LLCLD LLAL+EDF
Sbjct: 1021 LLLLNFTVVAVHKYGNFLNCHTCLDSFSGHICEAMLDVAIRSLFTKLLCLDALLALMEDF 1080

Query: 1081 IIDGRVLSCTDASFETLTEGILRVNIPIDGVNRTLSLAPASTNYLIAGSSILASISKAVH 1140
            +IDGRVLS TDASFETLT+G+LRVNIPID VNRTLSL PAST+YLIAGSSILASISKAVH
Sbjct: 1081 LIDGRVLSFTDASFETLTQGVLRVNIPIDSVNRTLSLTPASTDYLIAGSSILASISKAVH 1140

Query: 1141 RTGLLWEVSYSILRNRRCESSLVLTMLHIFAHIGGDQFFSLKGYSTLRAVLKSIIIHLE- 1200
            RTG+LWE+SY ILR+ R ESSL+LT+LHIFAHIGGDQFFSL+ YS LRAVLKSII HLE 
Sbjct: 1141 RTGILWEISYRILRSCRYESSLMLTILHIFAHIGGDQFFSLEVYSNLRAVLKSIITHLET 1200

Query: 1201 VRSSDDATFTPPKSNCRTEFVQCANCPFSDEVMSMPMVVSFLLQFIHKNISNEIMAGDLE 1260
            V SS+DATFTP K NCR EFVQCANCPFS+E M MPMVVSFLL+ + KNISNEIM  DLE
Sbjct: 1201 VGSSNDATFTPLKRNCRAEFVQCANCPFSEEGMPMPMVVSFLLRLLQKNISNEIMDEDLE 1260

Query: 1261 NPTSSLNSDSLFEKNIANRIPSKNSSGKLVHPASYLDCDASCCLKKYKVSDDESHSLFNP 1320
            N TSSLN +SLF++N+AN+IP KNSSGK VHP+ YLDCDASCCLKK+KVSDDE   LFNP
Sbjct: 1261 NSTSSLNLESLFKRNLANQIPCKNSSGKEVHPSVYLDCDASCCLKKFKVSDDEPRFLFNP 1320

Query: 1321 ILCDVTDAISLVELLACYMTWNWTFVNIISQLLELLKSSVKESLPIVILLGQLGRLGVAA 1380
             LCDVTDAISLVELLA YM WNWTF NII QL+ELLKSSVK+   IVILLGQLGR GV A
Sbjct: 1321 TLCDVTDAISLVELLAWYMGWNWTFANIIPQLMELLKSSVKKGFAIVILLGQLGRFGVVA 1380

Query: 1381 GGFEDGGVKILRSNLAAFLCLDSTIKSGLRVQIAIVSALLGLLPYDFETIVQDRVSYPAS 1440
            GGF+DGGVKILRSNL++FLCLD+TIKSGL VQIA VS+LLGLLP+DFETIVQD+V Y A 
Sbjct: 1381 GGFDDGGVKILRSNLSSFLCLDTTIKSGLPVQIATVSSLLGLLPFDFETIVQDKVRYRA- 1440

Query: 1441 SSSQYVEVKLIKTWFSLLSPKQKELSCNILQAAVCNVS 1455
            S +QY EV LIKTWFSLLSPKQKELSCNILQ A CNVS
Sbjct: 1441 SPNQYAEVNLIKTWFSLLSPKQKELSCNILQVAACNVS 1477

BLAST of Spg035871 vs. ExPASy TrEMBL
Match: A0A6J1KH58 (uncharacterized protein LOC111495215 OS=Cucurbita maxima OX=3661 GN=LOC111495215 PE=4 SV=1)

HSP 1 Score: 2172.5 bits (5628), Expect = 0.0e+00
Identity = 1176/1478 (79.57%), Postives = 1286/1478 (87.01%), Query Frame = 0

Query: 1    MVEDVVPKLESSNSCCKAWKNEYIKLDEKRNALRQAIKFLEQQITKIQAEKLNLQKGYEE 60
            MV DVV K ESSNSCCK WK+ Y KL+EKR ALRQA+K LE+QI KIQAE LNL++GYE+
Sbjct: 1    MVGDVVSKPESSNSCCKVWKDMYTKLEEKRIALRQAVKLLEEQIRKIQAENLNLKEGYEK 60

Query: 61   EKARASIEREGKDKESAIRVSLEREILDLKSQISSLRQNDVNAVNVRGEVEHLNALVAEG 120
            EKARASIERE KDKESAIRVSLEREI DLKSQISSLRQNDV AVNV GEV+HLN LVAEG
Sbjct: 61   EKARASIERESKDKESAIRVSLEREISDLKSQISSLRQNDVEAVNVHGEVDHLNVLVAEG 120

Query: 121  KKEICQLKELLGTEKRRTDVERKNAEARKEEAAQALKTVKIERSKASDLRKFHKTEMDKV 180
            KK+I QLKELL TEKRRTD ERKNAEARKEEAAQALKT+KIERSKASDL+K HKTEMDKV
Sbjct: 121  KKKISQLKELLETEKRRTDAERKNAEARKEEAAQALKTMKIERSKASDLKKLHKTEMDKV 180

Query: 181  NECRQQLEILKKEYEETKLKLASETSKLIEVKKDLEIEKKRTFKERERADSEMSKAEASR 240
            NE RQQL +L+KEYEETKLKLAS+TSKL EV KDLEIEK+RTFKE++RADSEMSKA+ASR
Sbjct: 181  NEFRQQLGMLEKEYEETKLKLASKTSKLTEVMKDLEIEKQRTFKEKKRADSEMSKAQASR 240

Query: 241  IQAEANRKQVEEEKSRAENLFQQLKRKTCKIEELQKQVKEHQTLKNFIESCCGQHDKKRD 300
            +Q E   KQV EEKS+AENLFQQL+RKTCKI++LQKQVKE +TLK FIESCCGQ  K+ +
Sbjct: 241  MQTEVTMKQVGEEKSKAENLFQQLERKTCKIKKLQKQVKEFKTLKKFIESCCGQPIKRTN 300

Query: 301  SKAVEKNDKPWLEMIQRNADELKLVFEFLKAKEVNIMHKMDGDLAIMKEKPVDSNMMKSS 360
            SK V+KNDKPWLEMIQRN +ELKL FE++KAKEVNI HKMD DLAIMKEK V+SNMMKSS
Sbjct: 301  SKDVKKNDKPWLEMIQRNENELKLAFEYVKAKEVNIKHKMDEDLAIMKEKTVNSNMMKSS 360

Query: 361  ELKNHLEIYRRKAMDEQCRADKLSLELEEKKRKVEELQKKLREFKSSRKLVDASSVSFEH 420
            ELKNHLEIYRRKAMDEQCRADKLSLELEEK RK+EELQK LR FKSSRKL DAS+VSFEH
Sbjct: 361  ELKNHLEIYRRKAMDEQCRADKLSLELEEKNRKIEELQKNLRGFKSSRKLADASAVSFEH 420

Query: 421  AMSSERAEMKLLKKKLKFEKTRLKHAREVANLEKTHRSIIQQELGRFKLEFVQLSNHLDN 480
            AMSSERAEMKLLKKKLKFEKTRLKHAR+VANLEK HRS+IQQELGRFKLEFVQLSNHLD+
Sbjct: 421  AMSSERAEMKLLKKKLKFEKTRLKHARQVANLEKNHRSVIQQELGRFKLEFVQLSNHLDD 480

Query: 481  LHKFASTGSKDNDDLEKTKNAENLQSLYSKKNLHAIEPFQTWMPDTFRRTTPQHGTPLFP 540
            LHKF+STG+KDNDD EKT NAE LQ  Y KKNL AIE FQ WMPDTFR+ TP HG PL P
Sbjct: 481  LHKFSSTGTKDNDDSEKTMNAEKLQRSYPKKNLRAIEAFQAWMPDTFRQATPHHGAPLLP 540

Query: 541  LSGGNHITSLSGIESWLESHPGDSNRKMLQSCAVNSSTASFSDGQLVGSQEKAGICLTAA 600
             S GNHITSLSGIES LES PGDSNRKMLQSCAVNSSTASFSDGQLVGSQE  G  LTA 
Sbjct: 541  SSVGNHITSLSGIESRLESFPGDSNRKMLQSCAVNSSTASFSDGQLVGSQEN-GFRLTAT 600

Query: 601  KLVGENLNMQPKISNVSGEVSKMKSNENLAIMAENSVRSPIKNHVGRANEKQQKRKRTVE 660
            KL GEN NMQP+ISN+S EVSKMKSNENLA+MA NSVRS IKN++GRANEKQ KRKRT+E
Sbjct: 601  KLAGENFNMQPRISNLSSEVSKMKSNENLAMMAGNSVRSHIKNNIGRANEKQGKRKRTIE 660

Query: 661  TVESIEYLYHESKKMHSQIEEKLSLLHALNSPTEKPLE---------------------K 720
            TVESI+YLYHESKKMHSQIEEKLSLLHALNSPTEKPL+                     K
Sbjct: 661  TVESIDYLYHESKKMHSQIEEKLSLLHALNSPTEKPLDKSEHVISNVLQDSCADKKIRKK 720

Query: 721  RKTSGRKKTRVQHLLDANEMKLNKVDTEVCTFQSIDRQPSQPFSKHTDSCQPCLEEPNNS 780
            RK   +KK +VQHLLD +EMKLNKVDTEVC  +SI  +PSQP SK  D+CQPC+EE N  
Sbjct: 721  RKALCQKKLKVQHLLDNSEMKLNKVDTEVCAPKSIGIKPSQPVSKLMDNCQPCVEELNTY 780

Query: 781  VISELQSLGTFGNIADGDYMKLLELDSAADEECYRRAMEMPLSPSLPDIYIPGAETSALN 840
            V SELQ+L TFGNIA+ DYMKLL+LDSAADEECYRRA+EMPLSP LP+IYI GAETSALN
Sbjct: 781  VRSELQTLETFGNIANVDYMKLLDLDSAADEECYRRAIEMPLSP-LPNIYIYGAETSALN 840

Query: 841  DLEPLVDELHKELPNEEEGQPQLHGHDVMDVEIKSNCTQSCNSGLLGDSHGSKRQLG--L 900
            + EPLVDELHKELP+E EGQP+ H + V+DVEIKSN TQSC+  LLGD H SKRQL   L
Sbjct: 841  EFEPLVDELHKELPDEREGQPKTHSYTVIDVEIKSNYTQSCDFDLLGDIHSSKRQLDPCL 900

Query: 901  WQGSRGSDLCDIVQAEKNCLDQIGVIVGMPGTNVPLSGCEGVGTSEIKSGTPDNSIPDFC 960
             QG + +DL DIVQA  NCLDQ+GVIVGMPGTNV LSGCEGVG SEIKSGT  NS PDFC
Sbjct: 901  IQGRQENDLFDIVQAGNNCLDQVGVIVGMPGTNVSLSGCEGVGASEIKSGTLGNSNPDFC 960

Query: 961  VLFSYTKDCHSISRIFSATTACIKRSSLTSHKEWMVQEILASLNMEHKLLPKEKTCVFFS 1020
            V+FS + DCHSI +IFSAT AC+KRSS+ + KEWMVQEILASLNMEH+L+PKEKTCVFFS
Sbjct: 961  VIFSNSNDCHSILKIFSATRACVKRSSIITQKEWMVQEILASLNMEHELVPKEKTCVFFS 1020

Query: 1021 LLVLNFTVVAGHKYGNFLNCNTCLDSFSGHICEAMLDVEIRSLFTELLCLDELLALIEDF 1080
            LL+LNFTVVA HKYGNFLNC+TCLDSFSGHICEAMLDV IRSLFT+LLCLD LLAL+EDF
Sbjct: 1021 LLLLNFTVVAVHKYGNFLNCHTCLDSFSGHICEAMLDVAIRSLFTKLLCLDALLALMEDF 1080

Query: 1081 IIDGRVLSCTDASFETLTEGILRVNIPIDGVNRTLSLAPASTNYLIAGSSILASISKAVH 1140
            +IDG+VLSCTDASFETLT+G+LRVNIPID VNRTLSL PAST+YLIAGSSILASISKAVH
Sbjct: 1081 LIDGQVLSCTDASFETLTQGVLRVNIPIDSVNRTLSLTPASTDYLIAGSSILASISKAVH 1140

Query: 1141 RTGLLWEVSYSILRNRRCESSLVLTMLHIFAHIGGDQFFSLKGYSTLRAVLKSIIIHLE- 1200
            RTGLLWE+SY ILR+ R ESSL+LT+LHIFAHIGGDQFFSL+ YS LRAVLKSII HLE 
Sbjct: 1141 RTGLLWEISYRILRSCRYESSLMLTILHIFAHIGGDQFFSLEVYSNLRAVLKSIITHLET 1200

Query: 1201 VRSSDDATFTPPKSNCRTEFVQCANCPFSDEVMSMPMVVSFLLQFIHKNISNEIMAGDLE 1260
            V SS+DATFTP K NCR EFVQCANCPFS+E MSMPMVVSFLLQ + KNISNEIM  DLE
Sbjct: 1201 VGSSNDATFTPLKRNCRAEFVQCANCPFSEEGMSMPMVVSFLLQLLPKNISNEIMDEDLE 1260

Query: 1261 NPTSSLNSDSLFEKNIANRIPSKNSSGKLVHPASYLDCDASCCLKKYKVSDDESHSLFNP 1320
            NPTSSLN +SLF++N+AN+IP KNSSGK VHP+ YLDCDASCCLKK+KVSDDE   LFNP
Sbjct: 1261 NPTSSLNLESLFKRNLANQIPCKNSSGKEVHPSVYLDCDASCCLKKFKVSDDEPRFLFNP 1320

Query: 1321 ILCDVTDAISLVELLACYMTWNWTFVNIISQLLELLKSSVKESLPIVILLGQLGRLGVAA 1380
             LCDVTDAISLVELLA YM WNWTF NII QL+ELLKSSV +   IVILLGQLGR GV A
Sbjct: 1321 TLCDVTDAISLVELLAWYMGWNWTFANIIPQLMELLKSSVTKGFAIVILLGQLGRFGVDA 1380

Query: 1381 GGFEDGGVKILRSNLAAFLCLDSTIKSGLRVQIAIVSALLGLLPYDFETIVQDRVSYPAS 1440
            GGFE+GGVKILRSNL++FLCLD+TIKSGL VQIA VS+LLGLLP+DFETIVQD+V   A 
Sbjct: 1381 GGFENGGVKILRSNLSSFLCLDTTIKSGLPVQIATVSSLLGLLPFDFETIVQDKVRCRA- 1440

Query: 1441 SSSQYVEVKLIKTWFSLLSPKQKELSCNILQAAVCNVS 1455
            SS+QYVEV LIK WFSLLSPKQKELSCNILQ A CNVS
Sbjct: 1441 SSNQYVEVNLIKMWFSLLSPKQKELSCNILQVAACNVS 1475

BLAST of Spg035871 vs. ExPASy TrEMBL
Match: A0A6J1GT99 (restin homolog isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111456908 PE=4 SV=1)

HSP 1 Score: 2150.9 bits (5572), Expect = 0.0e+00
Identity = 1162/1434 (81.03%), Postives = 1255/1434 (87.52%), Query Frame = 0

Query: 1    MVEDVVPKLESSNSCCKAWKNEYIKLDEKRNALRQAIKFLEQQITKIQAEKLNLQKGYEE 60
            M++D VP  ES+NSCCK WK++  +++EKRNALRQA+K L+QQI KIQAE LNL+KGYEE
Sbjct: 1    MLDDFVPNPESANSCCKRWKDKCTEVEEKRNALRQAVKLLQQQINKIQAENLNLKKGYEE 60

Query: 61   EKARASIEREGKDKESAIRVSLEREILDLKSQISSLRQNDVNAVNVRGEVEHLNALVAEG 120
            +KA ASIEREGK+KESAIRVSLEREILDLKS ISSLRQNDV+AV V  EVE LNALVAEG
Sbjct: 61   DKAGASIEREGKEKESAIRVSLEREILDLKSHISSLRQNDVDAVEVCREVEQLNALVAEG 120

Query: 121  KKEICQLKELLGTEKRRTDVERKNAEARKEEAAQALKTVKIERSKASDLRKFHKTEMDKV 180
            KKEI  L ELL TEKR+TD ERKNAE RKEEAAQALKTV+IERSKASDLRK HK E+DKV
Sbjct: 121  KKEISHLNELLETEKRKTDAERKNAEVRKEEAAQALKTVRIERSKASDLRKLHKNELDKV 180

Query: 181  NECRQQLEILKKEYEETKLKLASETSKLIEVKKDLEIEKKRTFKERERADSEMSKAEASR 240
             ECRQQLE+LKKEYEETKLKLASETSKL EVKKDLEIEK+RT KERERA+SEMSKA  SR
Sbjct: 181  KECRQQLEMLKKEYEETKLKLASETSKLNEVKKDLEIEKRRTSKERERANSEMSKAHVSR 240

Query: 241  IQAEANRKQVEEEKSRAENLFQQLKRKTCKIEELQKQVKEHQTLKNFIESCCGQHDKKRD 300
            IQAEANRKQ EEE+S+AENL QQL RKTCKIEELQKQVKE QTLK FIESCCGQHD+K D
Sbjct: 241  IQAEANRKQAEEEQSKAENLLQQLDRKTCKIEELQKQVKELQTLKTFIESCCGQHDEKTD 300

Query: 301  SKAVEKNDKPWLEMIQRNADELKLVFEFLKAKEVNIMHKMDGDLAIMKEKPVDSNMMKSS 360
             KAVEKN KPWLE+IQ+NA+E KL FEFLK KEVNIMHKMDGDLAIMKEKP+DSNMMKSS
Sbjct: 301  GKAVEKNVKPWLEVIQKNANEFKLAFEFLKDKEVNIMHKMDGDLAIMKEKPLDSNMMKSS 360

Query: 361  ELKNHLEIYRRKAMDEQCRADKLSLELEEKKRKVEELQKKLREFKSSRKLVDASSVSFEH 420
            ELK HLEIYR+KAMDEQ RADKL+LELEEKKRKVE+LQK LRE KSSRKLVDAS+VSFEH
Sbjct: 361  ELKKHLEIYRKKAMDEQYRADKLALELEEKKRKVEKLQKNLRELKSSRKLVDASAVSFEH 420

Query: 421  AMSSERAEMKLLKKKLKFEKTRLKHAREVANLEKTHRSIIQQELGRFKLEFVQLSNHLDN 480
            AMSSERAEMKLLKKKLKFEKTRLKHAREVANLE THRSIIQ ELG FKL+FVQLSN+LDN
Sbjct: 421  AMSSERAEMKLLKKKLKFEKTRLKHAREVANLENTHRSIIQHELGSFKLKFVQLSNYLDN 480

Query: 481  LHKFASTGSKDNDDLEKTKNAENLQSLYSKKNLHAIEPFQTWMPDTFRRTTPQHGTPLFP 540
            LHKFASTG+K NDDLEKTKNAENL+SLY++KNLHAIEPF+TW+PDTFR+TTPQH  PL P
Sbjct: 481  LHKFASTGAKGNDDLEKTKNAENLRSLYAEKNLHAIEPFKTWLPDTFRQTTPQHDAPLLP 540

Query: 541  LSGGNHITSLSGIESWLESHPGDSNRKMLQSCAVNSSTASFSDGQLVGSQEKAGICLTAA 600
            LSGGNH+TSLSGIES LE+HP +S+RKM QSCAVNSSTASFSDGQL GS+EKAG+CLTAA
Sbjct: 541  LSGGNHVTSLSGIESRLEAHPVNSDRKMFQSCAVNSSTASFSDGQLAGSREKAGLCLTAA 600

Query: 601  KLVGENLNMQPKISNVSGEVSKMKSNENLAIMAENSVRSPIKNHVGRANEKQQKRKRTVE 660
            KLVGENL M+PKISNVSGEVS+MK NEN A MAENSVRSPIKNHVGRANEKQQKRKRT+E
Sbjct: 601  KLVGENLIMKPKISNVSGEVSEMKDNEN-ARMAENSVRSPIKNHVGRANEKQQKRKRTIE 660

Query: 661  TVESIEYLYHESKKMHSQIEEKLSLLHALNSPTEKPLE---------------------K 720
             VE+IE LYHES+K+HSQIEEKLSLLHALNSPTEKPL+                     K
Sbjct: 661  AVENIECLYHESRKIHSQIEEKLSLLHALNSPTEKPLDKSGHVISNVFQDPSADKKARKK 720

Query: 721  RKTSGRKKTRVQHLLDANEMKLNKVDTEVCTFQSIDRQPSQPFSKHTDSCQPCLEEPNNS 780
            RKT  +KKTR Q LLD NEM+L+KV+ EVC  +S  RQPSQP SK TD+ QPCLEE NNS
Sbjct: 721  RKTLCQKKTREQ-LLDDNEMELSKVNIEVCALESFGRQPSQPVSKLTDNLQPCLEELNNS 780

Query: 781  VISELQSLGTFGNIADGDYMKLLELDSAADEECYRRAMEMPLSPSLPDIYIPGAETSALN 840
             ISELQ+LGT GNI+DGDYMKLL+LDSAADEECYRRAMEMPLSPSLPDIYIPGAETS LN
Sbjct: 781  AISELQTLGTLGNISDGDYMKLLDLDSAADEECYRRAMEMPLSPSLPDIYIPGAETSTLN 840

Query: 841  DLEPLVDELHKELPNEEEGQPQLHGHDVMDVEIKSNCTQSCNSGLLGDSHGSKRQLG--L 900
            + EPL+DE H      EEGQPQLH +DVMDVEIKSN TQ CNSGLLGD H SK  L    
Sbjct: 841  EFEPLLDERH------EEGQPQLHSYDVMDVEIKSNHTQYCNSGLLGDIHSSKHHLDPCF 900

Query: 901  WQGSRGSDLCDIVQAEKNCLDQIGVIVGMPGTNVPLSGCEGVGTSEIKSGTPDNSIPDFC 960
             QGS GSDLCDIVQA++N L+QIGV V MPGTNVPLSGCEGVG SEIKSGT DNSIPDFC
Sbjct: 901  MQGSHGSDLCDIVQAKENYLNQIGVTVEMPGTNVPLSGCEGVGASEIKSGTLDNSIPDFC 960

Query: 961  VLFSYTKDCHSISRIFSATTACIKRSSLTSHKEWMVQEILASLNMEHKLLPKEKTCVFFS 1020
            VLFS  KDC SISRIFSAT AC KRSSLT+ KEWMVQEILASLNMEHKLLP EK CVFFS
Sbjct: 961  VLFSNIKDCRSISRIFSATRACSKRSSLTNKKEWMVQEILASLNMEHKLLPMEKACVFFS 1020

Query: 1021 LLVLNFTVVAGHKYGNFLNCNTCLDSFSGHICEAMLDVEIRSLFTELLCLDELLALIEDF 1080
            LL+LNF VVA HKYGNFLNCNTCLDSFSGHICEAMLDVEIRSLFTE LCLDELL+LIEDF
Sbjct: 1021 LLLLNFNVVAVHKYGNFLNCNTCLDSFSGHICEAMLDVEIRSLFTESLCLDELLSLIEDF 1080

Query: 1081 IIDGRVLSCTDASFETLTEGILRVNIPIDGVNRTLSLAPASTNYLIAGSSILASISKAVH 1140
            IIDGR+LSC DAS ET  EG+LRVNI +DGVNR LSL PASTNYLIAGSSILASISKAV 
Sbjct: 1081 IIDGRILSCIDASLETSIEGVLRVNISVDGVNRALSLTPASTNYLIAGSSILASISKAVD 1140

Query: 1141 RTGLLWEVSYSILRNRRCESSLVLTMLHIFAHIGGDQFFSLKGYSTLRAVLKSIIIHLE- 1200
            RTG LWEVSYSILR  R ESSLVLTMLHIFAHIGGDQFFSL+GYSTL AVLKSII HLE 
Sbjct: 1141 RTGFLWEVSYSILRICRYESSLVLTMLHIFAHIGGDQFFSLEGYSTLMAVLKSIITHLEV 1200

Query: 1201 VRSSDDATFTPPKSNCRTEFVQCANCPFSDEVMSMPMVVSFLLQFIHKNISNEIMAGDLE 1260
            V SSDDA+FTPPK NCRTEFVQCA+CPFS  +MSMPM VSFLL+ +HKN     +A DLE
Sbjct: 1201 VGSSDDASFTPPKGNCRTEFVQCAHCPFSANIMSMPMAVSFLLRLVHKN----ALAEDLE 1260

Query: 1261 NPTSSLNSDSLFEKNIANRIPSKNSSGKLVHPASYLDCDASCCLKKYKVSDDESHSLFNP 1320
            NPT SLN +SL EKNIA +IP KN SG+ VHPA YLDCDASCCLKKY+VSDDES SLFNP
Sbjct: 1261 NPTGSLNPESLSEKNIAYQIPCKNLSGQEVHPALYLDCDASCCLKKYRVSDDESWSLFNP 1320

Query: 1321 ILCDVTDAISLVELLACYMTWNWTFVNIISQLLELLKSSVKESLPIVILLGQLGRLGVAA 1380
             LC++TDAISLVELLACYM WNWTF NIISQLLE LKSSVKESLP VILLGQLGR GVAA
Sbjct: 1321 SLCEITDAISLVELLACYMGWNWTFANIISQLLEFLKSSVKESLPFVILLGQLGRFGVAA 1380

Query: 1381 GGFEDGGVKILRSNLAAFLCLDSTIKSGLRVQIAIVSALLGLLPYDFETIVQDR 1411
            GGFEDGGVKILRSNL+AFL LD+TIKSGL VQIAIVSALLGLLP++FETI+QD+
Sbjct: 1381 GGFEDGGVKILRSNLSAFLYLDTTIKSGLCVQIAIVSALLGLLPFEFETIIQDK 1422

BLAST of Spg035871 vs. TAIR 10
Match: AT2G34780.1 (maternal effect embryo arrest 22 )

HSP 1 Score: 427.2 bits (1097), Expect = 5.4e-119
Identity = 455/1486 (30.62%), Postives = 706/1486 (47.51%), Query Frame = 0

Query: 7    PKLESSNSCCKAWKNEYIKLDEKRNALRQAIKFLEQQITKIQAEKLNLQKGYEEEKARAS 66
            P+L S N CC AW+ +YI + ++R+A ++ +  L++ I  + AEK NL++ + E     +
Sbjct: 8    PELASGNPCCLAWQGKYIGMKKRRDAFKEGVTLLQKAIENVNAEKSNLERKFGE----MA 67

Query: 67   IEREGKDKESAIRVSLEREILDLKSQISSLRQNDVNAVNVRGEVEHLNALVAEGK-KEIC 126
             + + K+  S ++ SLE+EI  LK +I SL+Q     +  + E   L    A G+ KEI 
Sbjct: 68   TDGDTKENGSTVKASLEKEISRLKFEIVSLQQKLERNLKEKSEETKLLQDQASGREKEIN 127

Query: 127  QLKELLGTEKRRTDVERKNAEARKEEAAQALKTVKIERSKASDLRKFHKTEMDKVNECRQ 186
            +L++LL  E  R       A++ +EE   A K    E +KA       K  + K  E  Q
Sbjct: 128  ELRDLLKKETLR-------ADSSEEEREHAFK----ELNKA-------KALIVKDEEIEQ 187

Query: 187  QLEILKKEYEETKLKLASETSKLIEVKKDLEIEKKRTFKERERADSEMSKAEASRIQAEA 246
             +  +K+E    K  LASE  K        E E+K+   E+++AD  +S+ E  R  A  
Sbjct: 188  DIPEVKREISLVKNLLASERQK-------TESERKKAESEKKKADKYLSELEVLRNSAHK 247

Query: 247  NRKQVEEEKSRAENLFQQLKRKTCKIEELQKQVKEHQTLKNFIESCCGQHDKKRDSKAVE 306
                +    S  E + +QL        EL+KQ    +TLK           K+ D ++ +
Sbjct: 248  TSSDLLTLTSNLETVKKQL--------ELEKQ----KTLK---------EKKRADMESAK 307

Query: 307  KNDKPWLEMIQRNADELKLVFEFLKAKEVNIMHKMDGDLAIMKEKPVDSNMMKSSELKNH 366
              D+       + A+++   FE ++A+   +  +M+   A  + K  + N  K  E    
Sbjct: 308  ARDQ------MKLAEDVSKKFEIVRARNEELKKEMESQTASSQVKFAE-NSEKLEEKIRL 367

Query: 367  LEIYRRKAMDEQCRADKLSLELEEKKRKVEELQKKLREFKSSRKLVDASSVSFEHAMSSE 426
            LE+ ++ AMD + R D L+ +L+E +   E L+K++ E   S+K +   S+S +     E
Sbjct: 368  LEMNKKTAMDWKSRTDDLTQQLQEAQLVAEGLKKQVHELSLSQKSIKTHSISPQKVRDLE 427

Query: 427  RAEMKLLKKKLKFEKTRLKHAREVANLEKTHRSIIQQELGRFKLEFVQLSNHLDNLHKFA 486
            +AEM+LLKKK+KFE+   KH++ VA  EK  R    +ELGR KLEF  L+N ++ L ++ 
Sbjct: 428  KAEMRLLKKKMKFERNCAKHSQTVAKFEKFRREFQCEELGRLKLEFGSLTNRMNLLDEYF 487

Query: 487  STGSKDNDDLEKTKNAENLQSLYSKKN----LHAIEPFQTWMPDTFRRTTPQHGTPLFPL 546
            ST  +    L K      L +L S+KN     H+    +      ++    +    L   
Sbjct: 488  STDVEGTAGLGKATGCRKLLTLNSQKNRNGEKHSDARCKLVASSGYQEQACKLSAHLISK 547

Query: 547  SGGNHITSLSGIESWLESHPGDSNRKMLQSCAVNSSTASFSDGQLVGSQEKAGICL-TAA 606
            SG     S+SG  S LES  G S +  L S  V SS  SFSDGQL+ SQ +    + T+A
Sbjct: 548  SGRGVSESVSGTISQLESPTGGSRK--LPSSGVISSATSFSDGQLLASQGREQFSVTTSA 607

Query: 607  KLVGENLNMQPKISNVSGEVSKMKSNENLAIMAENSVRSPIKNHVGRANEKQQKRKRTVE 666
            ++  +  N+QP  S++  ++S    N NL ++AEN ++   ++     +E  +KRKR +E
Sbjct: 608  EIAKDKPNIQPTKSSMLQKISDTSKNGNLCLVAENYLQRCQRD----IHENSRKRKRMLE 667

Query: 667  TVESIEYLYHESKKMHSQIEEKLSLLHAL-----NSPTEKP------------------- 726
             V S ++L    KK +  I EK+  L ++     + P+EK                    
Sbjct: 668  AVVSHKHLASGDKKKNLPIGEKMGTLQSMIVGTGSRPSEKEETLVPPDRQGGSSAIDITV 727

Query: 727  LEKRKTSGRKKTRVQHLLDANEMKLNKVDTEVCTFQSIDRQPSQPFSKHTDSCQPCLEEP 786
             +KR+ S +KK  VQ+ L+ N+                 + P     K T     CL   
Sbjct: 728  SKKRRVSCKKKIIVQNSLEFNQS---------------GKTPGNIAGKTT-----CLSTA 787

Query: 787  NNSVISELQSLGTFGNIADGDYMKLLELDSAADEECYRRAMEMPLSPSLPDIYIPGAETS 846
                +  L S     + A  DYMKLLELD+  +E  Y+ A E  LSP LP          
Sbjct: 788  TGHDVKTLFS----EDFAATDYMKLLELDNLEEENYYQMARESLLSPDLPQ--------- 847

Query: 847  ALNDLEPLVDELHKELPNEEEGQPQLHGHDVMDVEIKSNCTQSCNSGLLGDSHGSKRQLG 906
                    VD L  E+ NE++   +      +D+        + NS  L ++        
Sbjct: 848  --------VDFLGCEIMNEDKNPAR-----AIDL-------AASNSMYLRET-------- 907

Query: 907  LWQGSRGSDLCDIVQAEKNCLD--QIGVIVGMPGTNVPLSGCEGVGTSEIKSGTPDNSIP 966
                        I+ +E   L+   I V V MP    PL G                 + 
Sbjct: 908  ------------ILSSESPSLNTQNISVTVEMPPMLKPLHG----------------HLL 967

Query: 967  DFCVLFSYTKDCHSISRIFSATTACIKRSSLTSHKEWMVQEILASLNMEHKLLPKEKTCV 1026
               ++FS  +D +SI  I  AT  C++R    + ++W V  IL+SL ME  LL +E+ CV
Sbjct: 968  KHFIVFSNIEDQNSIIIIIHATNNCLQRCPSVTKEQWAVPAILSSLKMEENLLAQERACV 1027

Query: 1027 FFSLLVLNFTVVAGHKYGNFLNCN--TCLDSFSGHICEAMLDVEIRSLFTELLCLDELLA 1086
            F SLL+ NF++V   K GN LN +  +CLDSFS HI   M D E   + +     +ELL 
Sbjct: 1028 FLSLLLHNFSMVHTTKTGNTLNVDSFSCLDSFSKHIRGVMADTEAGVMLSGF--SEELLC 1087

Query: 1087 LIEDFIIDGRVLSCTDASFETLTEGILRVNIPIDGVNRTLSLAPASTNYLIAGSSILASI 1146
            L++D +   RVL    +S ET  E  L + + ++G N  L    A T+ L+AGS+ILA+I
Sbjct: 1088 LLQDLLSGQRVLFSVKSS-ET-CESDLSIPVTLNGENVALVNKIALTDQLVAGSAILAAI 1147

Query: 1147 SKAVHRTGLLWEVSYSILRNRRCE-SSLVLTMLHIFAHIGGDQFFSLKGYSTLRAVLKSI 1206
              A+ R G + E S+ IL     E +S++LT+LH+FA+I G++      +    AVLK I
Sbjct: 1148 CTALDRIGYICEASFEILHKYSHEKTSVLLTILHVFAYIAGEKMVLSSEHGISIAVLKYI 1207

Query: 1207 IIHLEVR---SSDDATFTPPKSNCRTEFVQCANCPFSDEVMSMPMVVSFLLQFIHKNISN 1266
            ++ LE +   + + ++   P  N          CPFSD   S+  + S L++ + +   +
Sbjct: 1208 VMFLENKHFGTVEGSSRLHPGKN---------KCPFSDRSSSLEAMASKLMEILQEFTES 1267

Query: 1267 EIMAGDLENPTSSLNSDSLFEKNIANRIPSKNSSGKLVHPASYLDCDASCCLKKYKVSDD 1326
              +   L   T SL S  L +                  PA     D  C L +     D
Sbjct: 1268 NTLHKSL---TGSLGSSHLEKTEF--------------RPAHK---DFQCVLTR-----D 1295

Query: 1327 ESHSLFNPILCDVTDAISLVELLACYMTWNWTFVNIISQLLELLKSSVKESLPIVI--LL 1386
            +S +     LCD+   +SLVEL+ACY  W+WT  NI++ LL++L   +  +L + I  LL
Sbjct: 1328 QSIN-----LCDI---LSLVELIACYTAWDWTSANIVAPLLKMLGMPLPMNLSVAIVSLL 1295

Query: 1387 GQLGRLGVAAGGFEDGGVKILRSNLAAFLCLDSTIKSGLRVQIAIVSALLGLLPYDFETI 1446
            GQL  +GV AGG+E+ G+  LR  L+AFL  ++T+K+G  VQIA VS+LL  L   F   
Sbjct: 1388 GQLSSIGVDAGGYENEGISNLRVKLSAFLQCETTLKAGFAVQIATVSSLLKTLQLKFPID 1295

Query: 1447 VQDRVSYPASSSSQYV--EVKLIKTWFSLLSPKQKELSCNILQAAV 1451
             QD+ +    S  Q +   V ++  W SLLS +Q+  +   LQ  V
Sbjct: 1448 FQDKTTMIPGSGDQSLSGSVNVVTKWLSLLSKEQRVFAFEFLQTNV 1295

BLAST of Spg035871 vs. TAIR 10
Match: AT2G34780.2 (maternal effect embryo arrest 22 )

HSP 1 Score: 390.2 bits (1001), Expect = 7.3e-108
Identity = 435/1421 (30.61%), Postives = 667/1421 (46.94%), Query Frame = 0

Query: 72   KDKESAIRVSLEREILDLKSQISSLRQNDVNAVNVRGEVEHLNALVAEGK-KEICQLKEL 131
            K+  S ++ SLE+EI  LK +I SL+Q     +  + E   L    A G+ KEI +L++L
Sbjct: 8    KENGSTVKASLEKEISRLKFEIVSLQQKLERNLKEKSEETKLLQDQASGREKEINELRDL 67

Query: 132  LGTEKRRTDVERKNAEARKEEAAQALKTVKIERSKASDLRKFHKTEMDKVNECRQQLEIL 191
            L  E  R       A++ +EE   A K    E +KA       K  + K  E  Q +  +
Sbjct: 68   LKKETLR-------ADSSEEEREHAFK----ELNKA-------KALIVKDEEIEQDIPEV 127

Query: 192  KKEYEETKLKLASETSKLIEVKKDLEIEKKRTFKERERADSEMSKAEASRIQAEANRKQV 251
            K+E    K  LASE  K        E E+K+   E+++AD  +S+ E  R  A      +
Sbjct: 128  KREISLVKNLLASERQK-------TESERKKAESEKKKADKYLSELEVLRNSAHKTSSDL 187

Query: 252  EEEKSRAENLFQQLKRKTCKIEELQKQVKEHQTLKNFIESCCGQHDKKRDSKAVEKNDKP 311
                S  E + +QL        EL+KQ    +TLK           K+ D ++ +  D+ 
Sbjct: 188  LTLTSNLETVKKQL--------ELEKQ----KTLK---------EKKRADMESAKARDQ- 247

Query: 312  WLEMIQRNADELKLVFEFLKAKEVNIMHKMDGDLAIMKEKPVDSNMMKSSELKNHLEIYR 371
                  + A+++   FE ++A+   +  +M+   A  + K  + N  K  E    LE+ +
Sbjct: 248  -----MKLAEDVSKKFEIVRARNEELKKEMESQTASSQVKFAE-NSEKLEEKIRLLEMNK 307

Query: 372  RKAMDEQCRADKLSLELEEKKRKVEELQKKLREFKSSRKLVDASSVSFEHAMSSERAEMK 431
            + AMD + R D L+ +L+E +   E L+K++ E   S+K +   S+S +     E+AEM+
Sbjct: 308  KTAMDWKSRTDDLTQQLQEAQLVAEGLKKQVHELSLSQKSIKTHSISPQKVRDLEKAEMR 367

Query: 432  LLKKKLKFEKTRLKHAREVANLEKTHRSIIQQELGRFKLEFVQLSNHLDNLHKFASTGSK 491
            LLKKK+KFE+   KH++ VA  EK  R    +ELGR KLEF  L+N ++ L ++ ST  +
Sbjct: 368  LLKKKMKFERNCAKHSQTVAKFEKFRREFQCEELGRLKLEFGSLTNRMNLLDEYFSTDVE 427

Query: 492  DNDDLEKTKNAENLQSLYSKKN----LHAIEPFQTWMPDTFRRTTPQHGTPLFPLSGGNH 551
                L K      L +L S+KN     H+    +      ++    +    L   SG   
Sbjct: 428  GTAGLGKATGCRKLLTLNSQKNRNGEKHSDARCKLVASSGYQEQACKLSAHLISKSGRGV 487

Query: 552  ITSLSGIESWLESHPGDSNRKMLQSCAVNSSTASFSDGQLVGSQEKAGICL-TAAKLVGE 611
              S+SG  S LES  G S +  L S  V SS  SFSDGQL+ SQ +    + T+A++  +
Sbjct: 488  SESVSGTISQLESPTGGSRK--LPSSGVISSATSFSDGQLLASQGREQFSVTTSAEIAKD 547

Query: 612  NLNMQPKISNVSGEVSKMKSNENLAIMAENSVRSPIKNHVGRANEKQQKRKRTVETVESI 671
              N+QP  S++  ++S    N NL ++AEN ++   ++     +E  +KRKR +E V S 
Sbjct: 548  KPNIQPTKSSMLQKISDTSKNGNLCLVAENYLQRCQRD----IHENSRKRKRMLEAVVSH 607

Query: 672  EYLYHESKKMHSQIEEKLSLLHAL-----NSPTEKP-------------------LEKRK 731
            ++L    KK +  I EK+  L ++     + P+EK                     +KR+
Sbjct: 608  KHLASGDKKKNLPIGEKMGTLQSMIVGTGSRPSEKEETLVPPDRQGGSSAIDITVSKKRR 667

Query: 732  TSGRKKTRVQHLLDANEMKLNKVDTEVCTFQSIDRQPSQPFSKHTDSCQPCLEEPNNSVI 791
             S +KK  VQ+ L+ N+                 + P     K T     CL       +
Sbjct: 668  VSCKKKIIVQNSLEFNQS---------------GKTPGNIAGKTT-----CLSTATGHDV 727

Query: 792  SELQSLGTFGNIADGDYMKLLELDSAADEECYRRAMEMPLSPSLPDIYIPGAETSALNDL 851
              L S     + A  DYMKLLELD+  +E  Y+ A E  LSP LP               
Sbjct: 728  KTLFS----EDFAATDYMKLLELDNLEEENYYQMARESLLSPDLPQ-------------- 787

Query: 852  EPLVDELHKELPNEEEGQPQLHGHDVMDVEIKSNCTQSCNSGLLGDSHGSKRQLGLWQGS 911
               VD L  E+ NE++   +      +D+        + NS  L ++             
Sbjct: 788  ---VDFLGCEIMNEDKNPAR-----AIDL-------AASNSMYLRET------------- 847

Query: 912  RGSDLCDIVQAEKNCLD--QIGVIVGMPGTNVPLSGCEGVGTSEIKSGTPDNSIPDFCVL 971
                   I+ +E   L+   I V V MP    PL G                 +    ++
Sbjct: 848  -------ILSSESPSLNTQNISVTVEMPPMLKPLHG----------------HLLKHFIV 907

Query: 972  FSYTKDCHSISRIFSATTACIKRSSLTSHKEWMVQEILASLNMEHKLLPKEKTCVFFSLL 1031
            FS  +D +SI  I  AT  C++R    + ++W V  IL+SL ME  LL +E+ CVF SLL
Sbjct: 908  FSNIEDQNSIIIIIHATNNCLQRCPSVTKEQWAVPAILSSLKMEENLLAQERACVFLSLL 967

Query: 1032 VLNFTVVAGHKYGNFLNCN--TCLDSFSGHICEAMLDVEIRSLFTELLCLDELLALIEDF 1091
            + NF++V   K GN LN +  +CLDSFS HI   M D E   + +     +ELL L++D 
Sbjct: 968  LHNFSMVHTTKTGNTLNVDSFSCLDSFSKHIRGVMADTEAGVMLSGF--SEELLCLLQDL 1027

Query: 1092 IIDGRVLSCTDASFETLTEGILRVNIPIDGVNRTLSLAPASTNYLIAGSSILASISKAVH 1151
            +   RVL    +S ET  E  L + + ++G N  L    A T+ L+AGS+ILA+I  A+ 
Sbjct: 1028 LSGQRVLFSVKSS-ET-CESDLSIPVTLNGENVALVNKIALTDQLVAGSAILAAICTALD 1087

Query: 1152 RTGLLWEVSYSILRNRRCE-SSLVLTMLHIFAHIGGDQFFSLKGYSTLRAVLKSIIIHLE 1211
            R G + E S+ IL     E +S++LT+LH+FA+I G++      +    AVLK I++ LE
Sbjct: 1088 RIGYICEASFEILHKYSHEKTSVLLTILHVFAYIAGEKMVLSSEHGISIAVLKYIVMFLE 1147

Query: 1212 VR---SSDDATFTPPKSNCRTEFVQCANCPFSDEVMSMPMVVSFLLQFIHKNISNEIMAG 1271
             +   + + ++   P  N          CPFSD   S+  + S L++ + +   +  +  
Sbjct: 1148 NKHFGTVEGSSRLHPGKN---------KCPFSDRSSSLEAMASKLMEILQEFTESNTLHK 1207

Query: 1272 DLENPTSSLNSDSLFEKNIANRIPSKNSSGKLVHPASYLDCDASCCLKKYKVSDDESHSL 1331
             L   T SL S  L +                  PA     D  C L +     D+S + 
Sbjct: 1208 SL---TGSLGSSHLEKTEF--------------RPAHK---DFQCVLTR-----DQSIN- 1234

Query: 1332 FNPILCDVTDAISLVELLACYMTWNWTFVNIISQLLELLKSSVKESLPIVI--LLGQLGR 1391
                LCD+   +SLVEL+ACY  W+WT  NI++ LL++L   +  +L + I  LLGQL  
Sbjct: 1268 ----LCDI---LSLVELIACYTAWDWTSANIVAPLLKMLGMPLPMNLSVAIVSLLGQLSS 1234

Query: 1392 LGVAAGGFEDGGVKILRSNLAAFLCLDSTIKSGLRVQIAIVSALLGLLPYDFETIVQDRV 1451
            +GV AGG+E+ G+  LR  L+AFL  ++T+K+G  VQIA VS+LL  L   F    QD+ 
Sbjct: 1328 IGVDAGGYENEGISNLRVKLSAFLQCETTLKAGFAVQIATVSSLLKTLQLKFPIDFQDKT 1234