Spg035789 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg035789 vs. NCBI nr
Match: XP_004135725.2 (berberine bridge enzyme-like 15 [Cucumis sativus] >KAE8653307.1 hypothetical protein Csa_023228 [Cucumis sativus])

HSP 1 Score: 895.6 bits (2313), Expect = 2.1e-256
Identity = 438/534 (82.02%), Postives = 487/534 (91.20%), Query Frame = 0

Query: 11  LLHLFFVALIWF-SSSYSLQ-ESFVECFNSTFYYSSARSIPISEVVFTNESASFSSLLQF 70
           L  LF+++ I   SSS+SLQ +SFVECFNST YY S +SIPIS+VVFTN+SA FSSLLQ 
Sbjct: 13  LFPLFYLSFICLSSSSHSLQADSFVECFNSTSYYYSPQSIPISKVVFTNKSAIFSSLLQS 72

Query: 71  SIRNLRFLNTSSPKPLFLVTPFHESHVQAAVVCAKNNGLQVRVRSGGHDYEGLSYVSTD- 130
           SI+NLRF NTS PKPLFLVTPFH+SHVQAA+VCA   G Q+R+RSGGHDYEG+SYVS+D 
Sbjct: 73  SIKNLRFFNTSLPKPLFLVTPFHQSHVQAAIVCANKKGFQIRLRSGGHDYEGISYVSSDQ 132

Query: 131 APFIVIDLNNLRSIDIDIKTKTASVETGATLGELYYRIAEKSPIHGFPAGSCPTVGVGGH 190
           + FI++DL+N RSIDID+KT+TA+VE GATLGELYYRIAEKSP HGFPAG+CPTVG+GGH
Sbjct: 133 SQFILLDLSNYRSIDIDMKTETATVEAGATLGELYYRIAEKSPTHGFPAGTCPTVGMGGH 192

Query: 191 VSGGGFGTLFRKYGLASDNVIDAKIVDFNGRILDRNSMGEDLFWAIRGGGGASFGVILSW 250
           VSGGGFGTLFRKYGLA+DNVIDAKIVDFNGRI+DRNSMGEDLFWAIRGGGGASFGVILSW
Sbjct: 193 VSGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSW 252

Query: 251 KLKLVYVPSNVTVFLVQRTLEQGAIQLFQKWQTIAHKLDEDLFLHVTAGVIEQNKETPNE 310
           KLKLVYVPSNVTVFLVQ+TLEQGAI LFQKWQTIAHKL EDLFLHVT GVI++  +TPN 
Sbjct: 253 KLKLVYVPSNVTVFLVQKTLEQGAIHLFQKWQTIAHKLHEDLFLHVTIGVIDEQNKTPNM 312

Query: 311 TSKTILLSFDSLFLGPIERLIPLMNSHFPELGLQRNNCTEMSWIQSVLYFAGLSTKSPLE 370
           +SKTIL+SF SLFLGP+ERLIPLMNSHFPELGL+RNNCTEMSWIQSVLYFAG+S ++P E
Sbjct: 313 SSKTILISFVSLFLGPVERLIPLMNSHFPELGLERNNCTEMSWIQSVLYFAGISIEAPPE 372

Query: 371 VLLKRPPLSNI-FFKAKSDFVTSPIPQTGLEALWAKMLEEEASFLILTPYGGKMSQISDS 430
           +LLKRPP+SN+ FFKAKSDFV SPIPQ GLE LW KMLEE ASFLIL+PYGGKM QISD 
Sbjct: 373 ILLKRPPISNVLFFKAKSDFVISPIPQIGLEGLWTKMLEEPASFLILSPYGGKMRQISDL 432

Query: 431 ETPFPHRRGNIFGIQYMVTWENANETYKHLSWIKELYAYMEPYVSKYPRAAYFNYRDLDL 490
           ETPFPHR+GN FGIQY+VTWENANETY+HLSWI+E+Y YMEPYVSKYPRAAY NYRDLDL
Sbjct: 433 ETPFPHRKGNTFGIQYLVTWENANETYRHLSWIREVYDYMEPYVSKYPRAAYLNYRDLDL 492

Query: 491 GRNYGRNTSYEEAKVWGLKYYKSNFDRLVRVKTKFDPSNFFWNEQSIPPSLYHS 541
           GRN GRNTSYEEAKVWGLKY+K+NFDRLVRVKTK DP NFFWNEQSIP S+YHS
Sbjct: 493 GRNCGRNTSYEEAKVWGLKYFKNNFDRLVRVKTKVDPLNFFWNEQSIPTSIYHS 546

BLAST of Spg035789 vs. NCBI nr
Match: XP_008446963.1 (PREDICTED: flavin-dependent oxidoreductase FOX5-like [Cucumis melo])

HSP 1 Score: 880.2 bits (2273), Expect = 9.3e-252
Identity = 437/535 (81.68%), Postives = 481/535 (89.91%), Query Frame = 0

Query: 11  LLHLFFVALIWF-SSSYSLQ-ESFVECFNSTFYYSSARSIPISEVVFTNE-SASFSSLLQ 70
           L  LF++  IW  SSSYSLQ ESF+ECFNST YY S +SIPIS+VVFT++ SA FSSLLQ
Sbjct: 12  LFLLFYLPFIWLSSSSYSLQAESFLECFNSTSYYYSPQSIPISKVVFTSKSSALFSSLLQ 71

Query: 71  FSIRNLRFLNTSSPKPLFLVTPFHESHVQAAVVCAKNNGLQVRVRSGGHDYEGLSYVSTD 130
            SI+NLRFLNTS  KPLFLVTPFH+SHVQ+A+VCA   GLQ+RVRSGGHDYEG+SYVS+D
Sbjct: 72  SSIKNLRFLNTSLQKPLFLVTPFHQSHVQSAIVCANKKGLQIRVRSGGHDYEGISYVSSD 131

Query: 131 -APFIVIDLNNLRSIDIDIKTKTASVETGATLGELYYRIAEKSPIHGFPAGSCPTVGVGG 190
            + FI++D  N RSIDID+KT+TA VE GATLGELYYRIAEKSPIHGFPAGSCPTVG+GG
Sbjct: 132 QSQFILLDFINFRSIDIDMKTETAIVEAGATLGELYYRIAEKSPIHGFPAGSCPTVGMGG 191

Query: 191 HVSGGGFGTLFRKYGLASDNVIDAKIVDFNGRILDRNSMGEDLFWAIRGGGGASFGVILS 250
           HV GGGFGTLFRKYGLA+DNVIDAKIVD NGRI+DRNSMGEDLFWAIRGGGGASFGVILS
Sbjct: 192 HVGGGGFGTLFRKYGLAADNVIDAKIVDVNGRIMDRNSMGEDLFWAIRGGGGASFGVILS 251

Query: 251 WKLKLVYVPSNVTVFLVQRTLEQGAIQLFQKWQTIAHKLDEDLFLHVTAGVIEQNKETPN 310
           WKLKLVYVPSNVTVF+VQ+TLEQGAI LFQKWQ IAHKL EDLFLHVT GVI++  +TPN
Sbjct: 252 WKLKLVYVPSNVTVFVVQKTLEQGAIHLFQKWQRIAHKLHEDLFLHVTMGVIDEQNKTPN 311

Query: 311 ETSKTILLSFDSLFLGPIERLIPLMNSHFPELGLQRNNCTEMSWIQSVLYFAGLSTKSPL 370
            +SKTIL+SF SLFLG IERLIPLMNSHFPELGL+RNNCTEMSWIQSVL+FAG+S ++P 
Sbjct: 312 MSSKTILISFVSLFLGRIERLIPLMNSHFPELGLERNNCTEMSWIQSVLFFAGISIETPP 371

Query: 371 EVLLKRPPLSN-IFFKAKSDFVTSPIPQTGLEALWAKMLEEEASFLILTPYGGKMSQISD 430
           +VLLKRPP S  +FFKAKSDFV SPIPQ G E LW KMLEEEAS+LILTPYGGKM QISD
Sbjct: 372 KVLLKRPPTSKMLFFKAKSDFVISPIPQIGFEGLWTKMLEEEASYLILTPYGGKMRQISD 431

Query: 431 SETPFPHRRGNIFGIQYMVTWENANETYKHLSWIKELYAYMEPYVSKYPRAAYFNYRDLD 490
            +TPFPHR+GNIFGIQYMVTWENANETY+HLSWI+E+Y YMEPYVSK+PRAAY NYRDLD
Sbjct: 432 FDTPFPHRKGNIFGIQYMVTWENANETYRHLSWIREVYDYMEPYVSKHPRAAYLNYRDLD 491

Query: 491 LGRNYGRNTSYEEAKVWGLKYYKSNFDRLVRVKTKFDPSNFFWNEQSIPPSLYHS 541
           LGRN GRNTSYEEAKVWGLKYYKSNFDRLVRVKTK DP NFFWNEQSIP S+YHS
Sbjct: 492 LGRNCGRNTSYEEAKVWGLKYYKSNFDRLVRVKTKVDPLNFFWNEQSIPTSVYHS 546

BLAST of Spg035789 vs. NCBI nr
Match: XP_022139883.1 (berberine bridge enzyme-like 13 [Momordica charantia])

HSP 1 Score: 875.2 bits (2260), Expect = 3.0e-250
Identity = 440/547 (80.44%), Postives = 483/547 (88.30%), Query Frame = 0

Query: 1   MMGMLLQWRV-LLHLFFVALIWF--SSSYSLQESFVECFNSTFYYSSARS---IPISEVV 60
           MMG+L  WR  L  LFFV  I+F  SS YS  +SF  CFNST YY S+ S   IPISEV+
Sbjct: 1   MMGILQYWRAHLFFLFFVVSIFFPSSSDYSQPQSFQXCFNSTSYYYSSHSIPIIPISEVL 60

Query: 61  FTNESASFSSLLQFSIRNLRFLNTSSPKPLFLVTPFHESHVQAAVVCAKNNGLQVRVRSG 120
           FTN+SASFSS+LQFSIRN RF NTSSPKPLFLVTPFHE HVQAA+VCA   GLQVRVRSG
Sbjct: 61  FTNKSASFSSVLQFSIRNFRFFNTSSPKPLFLVTPFHEFHVQAAIVCANKKGLQVRVRSG 120

Query: 121 GHDYEGLSYVSTDAPFIVIDLNNLRSIDIDIKTKTASVETGATLGELYYRIAEKSPIHGF 180
           GHDYEGLSY S+ APFIVIDL +LR+IDIDIKT+TASVETGATLGELYYRIAEKSPIHGF
Sbjct: 121 GHDYEGLSYTSSQAPFIVIDLIHLRAIDIDIKTETASVETGATLGELYYRIAEKSPIHGF 180

Query: 181 PAGSCPTVGVGGHVSGGGFGTLFRKYGLASDNVIDAKIVDFNGRILDRNSMGEDLFWAIR 240
           PAGSCPTVGVGGHVSGGGFGTLFRKYGLA+DN+IDAKIVDF GRI+DRNSMGEDLFWAIR
Sbjct: 181 PAGSCPTVGVGGHVSGGGFGTLFRKYGLAADNIIDAKIVDFKGRIMDRNSMGEDLFWAIR 240

Query: 241 GGGGASFGVILSWKLKLVYVPSNVTVFLVQRTLEQGAIQLFQKWQTIAHKLDEDLFLHVT 300
           GGG ASFGVILSWKLKLV VPS VTVFLVQ+TLEQGA QLFQKWQTIA KLDE LFLHV 
Sbjct: 241 GGGXASFGVILSWKLKLVSVPSKVTVFLVQKTLEQGATQLFQKWQTIADKLDEHLFLHVI 300

Query: 301 AGVIEQNKETPNETS-KTILLSFDSLFLGPIERLIPLMNSHFPELGLQRNNCTEMSWIQS 360
            GV E   ++P E S KTILLSF+SLFLGPI++L  +M++HFPELGLQR N TEMSWIQS
Sbjct: 301 IGVTENQNKSPKEPSTKTILLSFESLFLGPIDKLSLIMSTHFPELGLQRENYTEMSWIQS 360

Query: 361 VLYFAGLSTKSPLEVLLKRPPLSNIFFKAKSDFVTSPIPQTGLEALWAKMLEEEASFLIL 420
           VL+F GLST++PLE+LL+RPPLS+IFFKAKSD+VTSPIPQTGLEALW+K+LEEEAS LIL
Sbjct: 361 VLFFTGLSTEAPLEILLERPPLSDIFFKAKSDYVTSPIPQTGLEALWSKVLEEEASLLIL 420

Query: 421 TPYGGKMSQISDSETPFPHRRGNIFGIQYMVTWENANETYKHLSWIKELYAYMEPYVSKY 480
           TPYGGKMSQIS+SETPFPHRRG IFGIQYMVTWEN NET KHLSW++E+Y  ME YVSK 
Sbjct: 421 TPYGGKMSQISESETPFPHRRGIIFGIQYMVTWENGNETEKHLSWMREVYTCMELYVSKC 480

Query: 481 PRAAYFNYRDLDLGRNYGRNTSYEEAKVWGLKYYKSNFDRLVRVKTKFDPSNFFWNEQSI 540
           PR+AY +YRDLDLGRNYGRN SYEEAKVWGLKY+K NF+RLVRVKTK DPSN FWNEQSI
Sbjct: 481 PRSAYLSYRDLDLGRNYGRNRSYEEAKVWGLKYFKGNFERLVRVKTKVDPSNLFWNEQSI 540

BLAST of Spg035789 vs. NCBI nr
Match: XP_038895849.1 (berberine bridge enzyme-like 8 [Benincasa hispida])

HSP 1 Score: 848.2 bits (2190), Expect = 3.9e-242
Identity = 429/532 (80.64%), Postives = 466/532 (87.59%), Query Frame = 0

Query: 11  LLHLFFVALIWF-SSSYSLQESFVECFNSTFYYSSARSIPISEVVFTNESASFSSLLQFS 70
           L  LFFV+LIW  SSS SLQE F+ECFNST Y  S  SIPISEVVFTNESASFSSLLQ S
Sbjct: 9   LFLLFFVSLIWLCSSSDSLQERFLECFNSTSY--SKHSIPISEVVFTNESASFSSLLQLS 68

Query: 71  IRNLRFLNTSSPKPLFLVTPFHESHVQAAVVCAKNNGLQVRVRSGGHDYEGLSYVSTDAP 130
           IRNLRFL T+ PKPLFLVTPFHESHVQAA+VCA+  GLQVRVRSGGHDYEGLSYVS+ AP
Sbjct: 69  IRNLRFLTTTMPKPLFLVTPFHESHVQAAIVCARKKGLQVRVRSGGHDYEGLSYVSSQAP 128

Query: 131 FIVIDLNNLRSIDIDIKTKTASVETGATLGELYYRIAEKSPIHGFPAGSCPTVGVGGHVS 190
           FIVIDL NL SI IDI+T+T SVETGATLGELYY+IAEKSPIHGFPAGSCPTVGVGGHVS
Sbjct: 129 FIVIDLINLHSITIDIETETTSVETGATLGELYYKIAEKSPIHGFPAGSCPTVGVGGHVS 188

Query: 191 GGGFGTLFRKYGLASDNVIDAKIVDFNGRILDRNSMGEDLFWAIRGGGGASFGVILSWKL 250
           GGGFGTLFRKYGLA+DNVIDAKIVDFNGRI+DRNSMGEDLFWAIRGGGGASFGVILSWKL
Sbjct: 189 GGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKL 248

Query: 251 KLVYVPSNVTVFLVQRTLEQGAIQLFQKWQTIAHKLDEDLFLHVTAGVIEQNKETPNETS 310
           KLV VPS VTVF VQRTLEQGA  LFQKWQTIAHKLD+D+FLHVT  VI     T   + 
Sbjct: 249 KLVSVPSIVTVFNVQRTLEQGATHLFQKWQTIAHKLDQDIFLHVTTKVI-----TNFSSK 308

Query: 311 KTILLSFDSLFLGPIERLIPLMNSHFPELGLQRNNCTEMSWIQSVLYFAGLSTKSPLEVL 370
           KTI LSF SLFLGPIERLIP+M +HF ELGL+RN+CTEMSWIQSVL+FA  S ++PLEVL
Sbjct: 309 KTIRLSFTSLFLGPIERLIPIMKTHFSELGLKRNDCTEMSWIQSVLFFADFSIETPLEVL 368

Query: 371 LKR--PPLSNIFFKAKSDFVTSPIPQTGLEALWAKMLEEEASFLILTPYGGKMSQISDSE 430
           ++R  PP+S+ FF AKSD+VTSPI Q GLE LW K+LEEE S LILTPYGGKMSQIS+S+
Sbjct: 369 MERSSPPISDAFFTAKSDYVTSPISQNGLEGLWTKLLEEEKSELILTPYGGKMSQISESQ 428

Query: 431 TPFPHRRGNIFGIQYMVTWENANETYKHLSWIKELYAYMEPYVSKYPRAAYFNYRDLDLG 490
           TPFPHRRG+IFGIQY+ TW+NANET KHLSWI+E+Y YMEPYVSK PRAAY NYRDLDLG
Sbjct: 429 TPFPHRRGSIFGIQYLATWDNANETEKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDLG 488

Query: 491 RNYGRNTSYEEAKVWGLKYYKSNFDRLVRVKTKFDPSNFFWNEQSIPPSLYH 540
           RNY +NTSYEEAKVWGLKY+  NF+RLVRVKTK DP NFFWNEQSI P LYH
Sbjct: 489 RNYRKNTSYEEAKVWGLKYFSDNFERLVRVKTKVDPLNFFWNEQSI-PLLYH 532

BLAST of Spg035789 vs. NCBI nr
Match: XP_022140178.1 (berberine bridge enzyme-like 8 [Momordica charantia])

HSP 1 Score: 835.1 bits (2156), Expect = 3.4e-238
Identity = 420/542 (77.49%), Postives = 466/542 (85.98%), Query Frame = 0

Query: 2   MGMLLQWRVLLHLFFVALIWFSSSYSLQESFVECFNSTFYYSSARSIPISEVVFTNESAS 61
           MG+L   R+  HLF +     SSS  LQESF+ECFNST Y  S  S+PISEVVFTNESAS
Sbjct: 1   MGILHWGRI--HLFLIFFHCVSSSDCLQESFMECFNSTTY--SKHSVPISEVVFTNESAS 60

Query: 62  FSSLLQFSIRNLRFLNTSSPKPLFLVTPFHESHVQAAVVCAKNNGLQVRVRSGGHDYEGL 121
           FSSLL+ SIRNLRFL  SSPKP FLVTPF  SHVQAAVVCAK   LQVRVRSGGHDYEGL
Sbjct: 61  FSSLLRLSIRNLRFLTNSSPKPQFLVTPFRASHVQAAVVCAKKKSLQVRVRSGGHDYEGL 120

Query: 122 SYVSTDAPFIVIDLNNLRSIDIDIKTKTASVETGATLGELYYRIAEKSPIHGFPAGSCPT 181
           SYVS+  PFIVIDL NL SI IDI+T+TASVETGATLGE+YYRIAEKSPIHGFPAGSCPT
Sbjct: 121 SYVSSQTPFIVIDLINLSSIHIDIETETASVETGATLGEVYYRIAEKSPIHGFPAGSCPT 180

Query: 182 VGVGGHVSGGGFGTLFRKYGLASDNVIDAKIVDFNGRILDRNSMGEDLFWAIRGGGGASF 241
           VGVGGHVSGGGFGTLFRKYGLA+DNVIDAKIVDFNGRI DRNSMGEDLFWAIRGGGGASF
Sbjct: 181 VGVGGHVSGGGFGTLFRKYGLAADNVIDAKIVDFNGRIRDRNSMGEDLFWAIRGGGGASF 240

Query: 242 GVILSWKLKLVYVPSNVTVFLVQRTLEQGAIQLFQKWQTIAHKLDEDLFLHVTAGVIEQN 301
           GVILSWKLKLV VP +VTVF V RTL+QGA  LFQKWQ IAHK+D+DLFLHVT  V++  
Sbjct: 241 GVILSWKLKLVSVPPDVTVFNVHRTLDQGATHLFQKWQKIAHKMDQDLFLHVTTKVVDHK 300

Query: 302 ---KETPNETSKTILLSFDSLFLGPIERLIPLMNSHFPELGLQRNNCTEMSWIQSVLYFA 361
                +   +SKTI LSF SLFLGPIERLIPLMN+HF ELGL+RN+CTEMSWIQSVL+FA
Sbjct: 301 TKLSSSKEASSKTIKLSFTSLFLGPIERLIPLMNTHFHELGLKRNDCTEMSWIQSVLFFA 360

Query: 362 GLSTKSPLEVLLKR-PPLSNIFFKAKSDFVTSPIPQTGLEALWAKMLEEEASFLILTPYG 421
            LST++P E+L++R PP+S+ FF AKSD+VTSPI QTGLEALW+K+LEEE S LILTPYG
Sbjct: 361 DLSTETPPEILMERSPPISDTFFTAKSDYVTSPISQTGLEALWSKLLEEEKSELILTPYG 420

Query: 422 GKMSQISDSETPFPHRRGNIFGIQYMVTWENANETYKHLSWIKELYAYMEPYVSKYPRAA 481
           GKM QIS+SETPFPHRRGNI+GIQY+  WENANET KHLSW++E+Y+YM+P+VSKYPR A
Sbjct: 421 GKMDQISESETPFPHRRGNIYGIQYLAAWENANETEKHLSWMREVYSYMQPFVSKYPRGA 480

Query: 482 YFNYRDLDLGRNYGRNTSYEEAKVWGLKYYKSNFDRLVRVKTKFDPSNFFWNEQSIPPSL 540
           Y NYRDLDLGRNY RNTSY+EAKVWGLKY+K NF+RLVRVKTK DP NFFWNEQSIP   
Sbjct: 481 YLNYRDLDLGRNYERNTSYDEAKVWGLKYFKGNFERLVRVKTKVDPLNFFWNEQSIPLCH 538

BLAST of Spg035789 vs. ExPASy Swiss-Prot
Match: O64743 (Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV=1)

HSP 1 Score: 556.2 bits (1432), Expect = 4.0e-157
Identity = 277/531 (52.17%), Postives = 370/531 (69.68%), Query Frame = 0

Query: 14  LFFVALIWFS---SSYSLQESFVECFNSTFYYSSARSIPISEVVFT--NESASFSSLLQF 73
           LF V L+  S   SS +LQ+ FV+C       +S  S PI+   F+    +  F   L+ 
Sbjct: 12  LFLVTLLLISVPLSSSTLQQDFVKCLVD----NSDVSFPITASFFSPDQNATLFKEELES 71

Query: 74  SIRNLRFLNTSSPKPLFLVTPFHESHVQAAVVCAKNNGLQVRVRSGGHDYEGLSYVSTD- 133
           + +NLR+L  S+PKP+F+  P +E+HVQAAVVCAK   L +R+RSGGHDYEGLS+V+ D 
Sbjct: 72  TAQNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDE 131

Query: 134 APFIVIDLNNLRSIDIDIKTKTASVETGATLGELYYRIAEKSPIHGFPAGSCPTVGVGGH 193
            PF+++DL+ LR +D+D+ + +A    GAT+GE+YYRI EKS  HGFPAG C ++G+GGH
Sbjct: 132 TPFVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGH 191

Query: 194 VSGGGFGTLFRKYGLASDNVIDAKIVDFNGRILDRNSMGEDLFWAIRGGGGASFGVILSW 253
           + GG +G++ RK+GL +DNV+DA+IVD NG+ILDR +MGED+FWAIRGGGG SFGVIL+W
Sbjct: 192 LVGGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAW 251

Query: 254 KLKLVYVPSNVTVFLVQRTLEQGAIQLFQKWQTIAHKLDEDLFLHVTAGVIEQNKETPNE 313
           K+KLV VP+ VTVF V +TLEQ   ++  KW+ IA KLD+DLF+ V   +I    +T   
Sbjct: 252 KIKLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRV---IISPASKTTKP 311

Query: 314 TSKTILLSFDSLFLGPIERLIPLMNSHFPELGLQRNNCTEMSWIQSVLYFAGLSTKSPLE 373
            ++TI +S+ + FLG   RL+ +M   FPELGL + +CTEMSWI+SV+Y AG    +  E
Sbjct: 312 GNRTISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPE 371

Query: 374 VLLKRPPLSNIFFKAKSDFVTSPIPQTGLEALWAKMLEEEASFLILTPYGGKMSQISDSE 433
            LL    L    FKAKSDFV  PIP  GLE LW + LEE++   I  PYGG MS+IS+SE
Sbjct: 372 ALLAGKSLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLTIWNPYGGMMSRISESE 431

Query: 434 TPFPHRRGNIFGIQYMVTWENAN-ETYKHLSWIKELYAYMEPYVSKYPRAAYFNYRDLDL 493
            PFPHR G +F IQ++ TW++      +H+ WI+E+Y+YME YVSK PR AY NYRDLDL
Sbjct: 432 IPFPHRNGTLFKIQWLSTWQDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDL 491

Query: 494 GRNYGRNTSYEEAKVWGLKYYKSNFDRLVRVKTKFDPSNFFWNEQSIPPSL 538
           G N G      +A+ WG KYYK NF+RLV++K +FDP NFF +EQS+P  +
Sbjct: 492 GTNEGET----DAREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPTKI 531

BLAST of Spg035789 vs. ExPASy Swiss-Prot
Match: Q93ZA3 (Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=1 SV=1)

HSP 1 Score: 553.5 bits (1425), Expect = 2.6e-156
Identity = 276/533 (51.78%), Postives = 373/533 (69.98%), Query Frame = 0

Query: 15  FFVALIWFSSSY------SLQESFVECFNSTFYYSSARSIPISEVVFTNE--SASFSSLL 74
           F V L+  S SY      ++Q+ FV C       +S  S P+    FT++  ++SF   L
Sbjct: 12  FLVTLLLLSLSYIPLSFSTIQQDFVMCLVD----NSDASFPMDSSFFTHDLNASSFKLAL 71

Query: 75  QFSIRNLRFLNTSSPKPLFLVTPFHESHVQAAVVCAKNNGLQVRVRSGGHDYEGLSYVS- 134
           + S +NLR+L  S+PKP F+  P +E+HVQAAV+CAK   L +R+RSGGHDYEGLSYVS 
Sbjct: 72  ETSAQNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSE 131

Query: 135 TDAPFIVIDLNNLRSIDIDIKTKTASVETGATLGELYYRIAEKSPIHGFPAGSCPTVGVG 194
            +  F+++DL+ LR I +DI++ +A V  GA++GE+YYRI EKS IHGFPAG C ++G+G
Sbjct: 132 METAFVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIG 191

Query: 195 GHVSGGGFGTLFRKYGLASDNVIDAKIVDFNGRILDRNSMGEDLFWAIRGGGGASFGVIL 254
           GH+ GG +G++ RK+GL +DNV+DA+IVD +G+IL+R +MGED+FWAIRGGGG SFGVIL
Sbjct: 192 GHIIGGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVIL 251

Query: 255 SWKLKLVYVPSNVTVFLVQRTLEQGAIQLFQKWQTIAHKLDEDLFLHVTAGVIEQNKETP 314
           +WK+KLV VP  VTVF V RTLEQ   +L  KWQ +A KLDEDLF+ V   +I+   +TP
Sbjct: 252 AWKIKLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRV---IIQPTSKTP 311

Query: 315 NETSKTILLSFDSLFLGPIERLIPLMNSHFPELGLQRNNCTEMSWIQSVLYFAGLSTKSP 374
               +TI  S+   FLG   RL+ +M   FP+LGL + +C E SWI+SV+Y AG  + +P
Sbjct: 312 KSKERTISTSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAP 371

Query: 375 LEVLLKRPPLSNIFFKAKSDFVTSPIPQTGLEALWAKMLEEEASFLILTPYGGKMSQISD 434
            E LL    L   +FKAKSD+V  PIP  GLE LW K+LEE++   I  PYGG M++I +
Sbjct: 372 SEALLDGKSLFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEEDSPLTIWNPYGGMMAKIPE 431

Query: 435 SETPFPHRRGNIFGIQYMVTWENANET-YKHLSWIKELYAYMEPYVSKYPRAAYFNYRDL 494
           +ETPFPHR G +F IQ++  W++   +  KH+ W++E+Y+YME YVSK PR+AY NYRDL
Sbjct: 432 TETPFPHRSGTLFKIQWLTLWQDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDL 491

Query: 495 DLGRNYGRNTSYEEAKVWGLKYYKSNFDRLVRVKTKFDPSNFFWNEQSIPPSL 538
           DLG N G+ +   +A+ WG +Y+K NF+RLV +K KFDP NFF +EQSIP  L
Sbjct: 492 DLGMN-GKGS---DAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIPTEL 533

BLAST of Spg035789 vs. ExPASy Swiss-Prot
Match: Q9FKV0 (Berberine bridge enzyme-like 24 OS=Arabidopsis thaliana OX=3702 GN=At5g44380 PE=2 SV=1)

HSP 1 Score: 542.0 bits (1395), Expect = 7.9e-153
Identity = 271/535 (50.65%), Postives = 372/535 (69.53%), Query Frame = 0

Query: 11  LLHLFFVALIWFSSSYSLQESFVECFNSTFYYSSARSIPISEVVFT--NESASFSSLLQF 70
           +L+  F  +   SSS S+Q+ F+ C     +     S P+ + +FT     + F+ +L+ 
Sbjct: 17  VLYFSFYCITPTSSSASIQDQFINCVKRNTHV----SFPLEKTLFTPAKNVSLFNQVLES 76

Query: 71  SIRNLRFLNTSSPKPLFLVTPFHESHVQAAVVCAKNNGLQVRVRSGGHDYEGLSYVS-TD 130
           + +NL+FL  S PKP F+  P H+S VQA+++C+K  G+  RVRSGGHD+E LSYVS  +
Sbjct: 77  TAQNLQFLAKSMPKPGFIFRPIHQSQVQASIICSKKLGIHFRVRSGGHDFEALSYVSRIE 136

Query: 131 APFIVIDLNNLRSIDIDIKTKTASVETGATLGELYYRIAEKSPIHGFPAGSCPTVGVGGH 190
            PFI++DL+ L+ I++DI++ +A V+ GATLGELYYRIAEKS IHGFPAG C +VG+GG+
Sbjct: 137 KPFILLDLSKLKQINVDIESNSAWVQPGATLGELYYRIAEKSKIHGFPAGLCTSVGIGGY 196

Query: 191 VSGGGFGTLFRKYGLASDNVIDAKIVDFNGRILDRNSMGEDLFWAIRGGGGASFGVILSW 250
           ++GGG+GTL RKYGLA DNV+D K+VD NG++LDR +MGEDLFWAIRGGGGASFG++L+W
Sbjct: 197 MTGGGYGTLMRKYGLAGDNVLDVKMVDANGKLLDRAAMGEDLFWAIRGGGGASFGIVLAW 256

Query: 251 KLKLVYVPSNVTVFLVQRTLEQGA-IQLFQKWQTIAHKLDEDLFLHVTAGVIEQNKETPN 310
           K+KLV VP  VTVF V +TLEQ A ++   KWQ I+ K+ E++ + V        +   N
Sbjct: 257 KIKLVPVPKTVTVFTVTKTLEQDARLKTISKWQQISSKIIEEIHIRVVL------RAAGN 316

Query: 311 ETSKTILLSFDSLFLGPIERLIPLMNSHFPELGLQRNNCTEMSWIQSVLYFAGLSTKSPL 370
           + +KT+ +++   FLG    L+ +M   FPELGL + +CTEMSWI++ L+  G  T SP+
Sbjct: 317 DGNKTVTMTYLGQFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHGGFPTGSPI 376

Query: 371 EVLLK-RPPLSNIFFKAKSDFVTSPIPQTGLEALWAKMLEEEASFLILTPYGGKMSQISD 430
           E+LL+ + PL   +FKA SDFV  PIP  GL+ ++ +++E   +FL  TPYGG MS+I +
Sbjct: 377 EILLQLKSPLGKDYFKATSDFVKEPIPVIGLKGIFKRLIEGNTTFLNWTPYGGMMSKIPE 436

Query: 431 SETPFPHRRGNIFGIQYMVTW-ENANETYKHLSWIKELYAYMEPYVSKYPRAAYFNYRDL 490
           S  PFPHR G +F I Y   W EN   + + ++WIKE+Y YM PYVS  PR AY NYRDL
Sbjct: 437 SAIPFPHRNGTLFKILYYANWLENDKTSSRKINWIKEIYNYMAPYVSSNPRQAYVNYRDL 496

Query: 491 DLGRNYGR-NTSYEEAKVWGLKYYKSNFDRLVRVKTKFDPSNFFWNEQSIPPSLY 539
           D G+N      ++ EAK+WG KY+K NFDRLV++KTK DP NFF +EQSIPP  Y
Sbjct: 497 DFGQNKNNAKVNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQSIPPMPY 541

BLAST of Spg035789 vs. ExPASy Swiss-Prot
Match: Q9FKU9 (Berberine bridge enzyme-like 25 OS=Arabidopsis thaliana OX=3702 GN=At5g44390 PE=2 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 5.1e-152
Identity = 273/524 (52.10%), Postives = 368/524 (70.23%), Query Frame = 0

Query: 20  IWFSSSYSLQESFVECFNSTFYYSSARSIPISEVVFTNE--SASFSSLLQFSIRNLRFLN 79
           I  +SS SLQ+ F++C     Y ++     + +  FT E  ++ F+ +L+ + +N R+L 
Sbjct: 25  ITLTSSTSLQDDFIKC----LYRNTNVRFTLDKTFFTPERNASIFTEVLESTAQNQRYLT 84

Query: 80  TSSPKPLFLVTPFHESHVQAAVVCAKNNGLQVRVRSGGHDYEGLSYVS-TDAPFIVIDLN 139
            + PKP F+  P HESHVQA+V+C+K   +  RVRSGGHDYEG+SYVS  + PF++IDL+
Sbjct: 85  KTMPKPGFIFKPVHESHVQASVICSKKLEIHFRVRSGGHDYEGVSYVSQIEKPFVLIDLS 144

Query: 140 NLRSIDIDIKTKTASVETGATLGELYYRIAEKSPIHGFPAGSCPTVGVGGHVSGGGFGTL 199
            LR I++DIK  +A VE GAT+GELYYRIAEKS  HGFPAG  P++G+GGH++GG +G+L
Sbjct: 145 KLRQINVDIKDTSAWVEAGATVGELYYRIAEKSKFHGFPAGVYPSLGIGGHITGGAYGSL 204

Query: 200 FRKYGLASDNVIDAKIVDFNGRILDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVYVPS 259
            RKYGLA+DNV+DAKIVD NG++LDR SMGEDLFWAIRGG G SFG+ILSWK+KLV VP 
Sbjct: 205 MRKYGLAADNVLDAKIVDANGKLLDRASMGEDLFWAIRGGSGGSFGIILSWKIKLVPVPE 264

Query: 260 NVTVFLVQRTLEQG-AIQLFQKWQTIAHKLDEDLFLHVTAGVIEQNKETPNETSKTILLS 319
            +TVF V +T EQ  + ++  KWQ IA  L ++LFL V   V      + N+ +KT+ ++
Sbjct: 265 TLTVFTVTKTFEQDRSFKILSKWQEIADNLVDELFLRVFFTV------SGNKANKTVTMA 324

Query: 320 FDSLFLGPIERLIPLMNSHFPELGLQRNNCTEMSWIQSVLYFAGLSTK--SPLEVLLK-R 379
           +   FLG    L+ +M   FPELGL + +C EMSWI S++Y +G  T    P+E+LL+ +
Sbjct: 325 YIGQFLGEKGTLMEVMKKDFPELGLTQKDCIEMSWIDSIIYNSGFPTNPPPPIEILLQAK 384

Query: 380 PPLSNIFFKAKSDFVTSPIPQTGLEALWAKMLEEEASFLILTPYGGKMSQISDSETPFPH 439
            P+  ++FK KSDF   PIP  GLE ++ K+LEE+A+ +I TPYGGKM +I +SE PFPH
Sbjct: 385 SPIGKVYFKGKSDFAKKPIPVLGLEGMFKKLLEEDAALVIWTPYGGKMDKIPESEIPFPH 444

Query: 440 RRGNIFGIQYMVTWENANE-TYKHLSWIKELYAYMEPYVSKYPRAAYFNYRDLDLGRNYG 499
           R G  F IQY  +W ++ +   +   WI+ELY YM PYVS  PR AY NYRDLDLG+N  
Sbjct: 445 RNGTNFMIQYYRSWSDSEKRPNRRTKWIRELYGYMTPYVSSNPRQAYVNYRDLDLGQNKD 504

Query: 500 RNTS-YEEAKVWGLKYYKSNFDRLVRVKTKFDPSNFFWNEQSIP 535
            + S + EAK+WG  Y+K NF+RLVR+K+K DP NFF +EQSIP
Sbjct: 505 NSKSNFIEAKIWGANYFKDNFNRLVRIKSKVDPDNFFRHEQSIP 538

BLAST of Spg035789 vs. ExPASy Swiss-Prot
Match: Q9SA85 (Berberine bridge enzyme-like 8 OS=Arabidopsis thaliana OX=3702 GN=At1g30700 PE=2 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 5.1e-152
Identity = 269/533 (50.47%), Postives = 370/533 (69.42%), Query Frame = 0

Query: 6   LQWRVLLHLFFVALIWFSSSYSL-QESFVECFNSTFYYSSARSIPISEVVFTNESASFSS 65
           +++ ++L LFFV  IW SSS S   E+F +C  S    +S    PIS  +F + + S+SS
Sbjct: 1   MKYALILVLFFVVFIWQSSSSSANSETFTQCLTS----NSDPKHPISPAIFFSGNGSYSS 60

Query: 66  LLQFSIRNLRFLNTSSPKPLFLVTPFHESHVQAAVVCAKNNGLQVRVRSGGHDYEGLSYV 125
           +LQ +IRNLRF  TS+PKP  ++   HESHVQAA+ C K + LQ+++RSGGHDY+GLSYV
Sbjct: 61  VLQANIRNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYV 120

Query: 126 S-TDAPFIVIDLNNLRSIDIDIKTKTASVETGATLGELYYRIAEKSPIHGFPAGSCPTVG 185
           + +  PF V+D+ NLRS+D+D+ +KTA V+TGA LGE+YY I EKS    +PAG CPTVG
Sbjct: 121 TYSGKPFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVG 180

Query: 186 VGGHVSGGGFGTLFRKYGLASDNVIDAKIVDFNGRILDRNSMGEDLFWAIRGGGGASFGV 245
           VGGH+SGGG+G + RKYGL  DN IDA++VD NG+ILDR  MGEDL+WAI GGGG S+GV
Sbjct: 181 VGGHISGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGV 240

Query: 246 ILSWKLKLVYVPSNVTVFLVQRTLEQGAIQLFQKWQTIAHKLDEDLFLHVTAGVIEQNKE 305
           +L++K+ LV VP NVTVF + RTLEQ A  +  +WQ +A KL ++LF+     V+     
Sbjct: 241 VLAYKINLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTV- 300

Query: 306 TPNETSKTILLSFDSLFLGPIERLIPLMNSHFPELGLQRNNCTEMSWIQSVLYFAGLSTK 365
               + KT+  +F ++FLG    L+ ++N  FPELGL R++CTE SWIQSVL++  +   
Sbjct: 301 ---SSQKTVRTTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVG 360

Query: 366 SPLEVLLKRPPLSNIFFKAKSDFVTSPIPQTGLEALWAKMLEEEASFLILTPYGGKMSQI 425
           S   +LL+R    N + K KSD+V  PI +TGLE++W KM+E E   +   PYGG+M +I
Sbjct: 361 SSETLLLQRNQPVN-YLKRKSDYVREPISRTGLESIWKKMIELEIPTMAFNPYGGEMGRI 420

Query: 426 SDSETPFPHRRGNIFGIQYMVTWENANETYKHLSWIKELYAYMEPYVSKYPRAAYFNYRD 485
           S + TPFP+R GN++ IQY   W +   T +++   ++LY +M P+VSK PR ++FNYRD
Sbjct: 421 SSTVTPFPYRAGNLWKIQYGANWRDETLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRD 480

Query: 486 LDLGRN--YGRNTSYEEAKVWGLKYYKSNFDRLVRVKTKFDPSNFFWNEQSIP 535
           +DLG N   G+ +SY E K +G KY+  NF+RLV++KT+ D  NFF NEQSIP
Sbjct: 481 VDLGINSHNGKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIP 524

BLAST of Spg035789 vs. ExPASy TrEMBL
Match: A0A1S3BH69 (flavin-dependent oxidoreductase FOX5-like OS=Cucumis melo OX=3656 GN=LOC103489514 PE=3 SV=1)

HSP 1 Score: 880.2 bits (2273), Expect = 4.5e-252
Identity = 437/535 (81.68%), Postives = 481/535 (89.91%), Query Frame = 0

Query: 11  LLHLFFVALIWF-SSSYSLQ-ESFVECFNSTFYYSSARSIPISEVVFTNE-SASFSSLLQ 70
           L  LF++  IW  SSSYSLQ ESF+ECFNST YY S +SIPIS+VVFT++ SA FSSLLQ
Sbjct: 12  LFLLFYLPFIWLSSSSYSLQAESFLECFNSTSYYYSPQSIPISKVVFTSKSSALFSSLLQ 71

Query: 71  FSIRNLRFLNTSSPKPLFLVTPFHESHVQAAVVCAKNNGLQVRVRSGGHDYEGLSYVSTD 130
            SI+NLRFLNTS  KPLFLVTPFH+SHVQ+A+VCA   GLQ+RVRSGGHDYEG+SYVS+D
Sbjct: 72  SSIKNLRFLNTSLQKPLFLVTPFHQSHVQSAIVCANKKGLQIRVRSGGHDYEGISYVSSD 131

Query: 131 -APFIVIDLNNLRSIDIDIKTKTASVETGATLGELYYRIAEKSPIHGFPAGSCPTVGVGG 190
            + FI++D  N RSIDID+KT+TA VE GATLGELYYRIAEKSPIHGFPAGSCPTVG+GG
Sbjct: 132 QSQFILLDFINFRSIDIDMKTETAIVEAGATLGELYYRIAEKSPIHGFPAGSCPTVGMGG 191

Query: 191 HVSGGGFGTLFRKYGLASDNVIDAKIVDFNGRILDRNSMGEDLFWAIRGGGGASFGVILS 250
           HV GGGFGTLFRKYGLA+DNVIDAKIVD NGRI+DRNSMGEDLFWAIRGGGGASFGVILS
Sbjct: 192 HVGGGGFGTLFRKYGLAADNVIDAKIVDVNGRIMDRNSMGEDLFWAIRGGGGASFGVILS 251

Query: 251 WKLKLVYVPSNVTVFLVQRTLEQGAIQLFQKWQTIAHKLDEDLFLHVTAGVIEQNKETPN 310
           WKLKLVYVPSNVTVF+VQ+TLEQGAI LFQKWQ IAHKL EDLFLHVT GVI++  +TPN
Sbjct: 252 WKLKLVYVPSNVTVFVVQKTLEQGAIHLFQKWQRIAHKLHEDLFLHVTMGVIDEQNKTPN 311

Query: 311 ETSKTILLSFDSLFLGPIERLIPLMNSHFPELGLQRNNCTEMSWIQSVLYFAGLSTKSPL 370
            +SKTIL+SF SLFLG IERLIPLMNSHFPELGL+RNNCTEMSWIQSVL+FAG+S ++P 
Sbjct: 312 MSSKTILISFVSLFLGRIERLIPLMNSHFPELGLERNNCTEMSWIQSVLFFAGISIETPP 371

Query: 371 EVLLKRPPLSN-IFFKAKSDFVTSPIPQTGLEALWAKMLEEEASFLILTPYGGKMSQISD 430
           +VLLKRPP S  +FFKAKSDFV SPIPQ G E LW KMLEEEAS+LILTPYGGKM QISD
Sbjct: 372 KVLLKRPPTSKMLFFKAKSDFVISPIPQIGFEGLWTKMLEEEASYLILTPYGGKMRQISD 431

Query: 431 SETPFPHRRGNIFGIQYMVTWENANETYKHLSWIKELYAYMEPYVSKYPRAAYFNYRDLD 490
            +TPFPHR+GNIFGIQYMVTWENANETY+HLSWI+E+Y YMEPYVSK+PRAAY NYRDLD
Sbjct: 432 FDTPFPHRKGNIFGIQYMVTWENANETYRHLSWIREVYDYMEPYVSKHPRAAYLNYRDLD 491

Query: 491 LGRNYGRNTSYEEAKVWGLKYYKSNFDRLVRVKTKFDPSNFFWNEQSIPPSLYHS 541
           LGRN GRNTSYEEAKVWGLKYYKSNFDRLVRVKTK DP NFFWNEQSIP S+YHS
Sbjct: 492 LGRNCGRNTSYEEAKVWGLKYYKSNFDRLVRVKTKVDPLNFFWNEQSIPTSVYHS 546

BLAST of Spg035789 vs. ExPASy TrEMBL
Match: A0A6J1CGQ8 (berberine bridge enzyme-like 13 OS=Momordica charantia OX=3673 GN=LOC111010686 PE=3 SV=1)

HSP 1 Score: 875.2 bits (2260), Expect = 1.4e-250
Identity = 440/547 (80.44%), Postives = 483/547 (88.30%), Query Frame = 0

Query: 1   MMGMLLQWRV-LLHLFFVALIWF--SSSYSLQESFVECFNSTFYYSSARS---IPISEVV 60
           MMG+L  WR  L  LFFV  I+F  SS YS  +SF  CFNST YY S+ S   IPISEV+
Sbjct: 1   MMGILQYWRAHLFFLFFVVSIFFPSSSDYSQPQSFQXCFNSTSYYYSSHSIPIIPISEVL 60

Query: 61  FTNESASFSSLLQFSIRNLRFLNTSSPKPLFLVTPFHESHVQAAVVCAKNNGLQVRVRSG 120
           FTN+SASFSS+LQFSIRN RF NTSSPKPLFLVTPFHE HVQAA+VCA   GLQVRVRSG
Sbjct: 61  FTNKSASFSSVLQFSIRNFRFFNTSSPKPLFLVTPFHEFHVQAAIVCANKKGLQVRVRSG 120

Query: 121 GHDYEGLSYVSTDAPFIVIDLNNLRSIDIDIKTKTASVETGATLGELYYRIAEKSPIHGF 180
           GHDYEGLSY S+ APFIVIDL +LR+IDIDIKT+TASVETGATLGELYYRIAEKSPIHGF
Sbjct: 121 GHDYEGLSYTSSQAPFIVIDLIHLRAIDIDIKTETASVETGATLGELYYRIAEKSPIHGF 180

Query: 181 PAGSCPTVGVGGHVSGGGFGTLFRKYGLASDNVIDAKIVDFNGRILDRNSMGEDLFWAIR 240
           PAGSCPTVGVGGHVSGGGFGTLFRKYGLA+DN+IDAKIVDF GRI+DRNSMGEDLFWAIR
Sbjct: 181 PAGSCPTVGVGGHVSGGGFGTLFRKYGLAADNIIDAKIVDFKGRIMDRNSMGEDLFWAIR 240

Query: 241 GGGGASFGVILSWKLKLVYVPSNVTVFLVQRTLEQGAIQLFQKWQTIAHKLDEDLFLHVT 300
           GGG ASFGVILSWKLKLV VPS VTVFLVQ+TLEQGA QLFQKWQTIA KLDE LFLHV 
Sbjct: 241 GGGXASFGVILSWKLKLVSVPSKVTVFLVQKTLEQGATQLFQKWQTIADKLDEHLFLHVI 300

Query: 301 AGVIEQNKETPNETS-KTILLSFDSLFLGPIERLIPLMNSHFPELGLQRNNCTEMSWIQS 360
            GV E   ++P E S KTILLSF+SLFLGPI++L  +M++HFPELGLQR N TEMSWIQS
Sbjct: 301 IGVTENQNKSPKEPSTKTILLSFESLFLGPIDKLSLIMSTHFPELGLQRENYTEMSWIQS 360

Query: 361 VLYFAGLSTKSPLEVLLKRPPLSNIFFKAKSDFVTSPIPQTGLEALWAKMLEEEASFLIL 420
           VL+F GLST++PLE+LL+RPPLS+IFFKAKSD+VTSPIPQTGLEALW+K+LEEEAS LIL
Sbjct: 361 VLFFTGLSTEAPLEILLERPPLSDIFFKAKSDYVTSPIPQTGLEALWSKVLEEEASLLIL 420

Query: 421 TPYGGKMSQISDSETPFPHRRGNIFGIQYMVTWENANETYKHLSWIKELYAYMEPYVSKY 480
           TPYGGKMSQIS+SETPFPHRRG IFGIQYMVTWEN NET KHLSW++E+Y  ME YVSK 
Sbjct: 421 TPYGGKMSQISESETPFPHRRGIIFGIQYMVTWENGNETEKHLSWMREVYTCMELYVSKC 480

Query: 481 PRAAYFNYRDLDLGRNYGRNTSYEEAKVWGLKYYKSNFDRLVRVKTKFDPSNFFWNEQSI 540
           PR+AY +YRDLDLGRNYGRN SYEEAKVWGLKY+K NF+RLVRVKTK DPSN FWNEQSI
Sbjct: 481 PRSAYLSYRDLDLGRNYGRNRSYEEAKVWGLKYFKGNFERLVRVKTKVDPSNLFWNEQSI 540

BLAST of Spg035789 vs. ExPASy TrEMBL
Match: A0A6J1CHF0 (berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111010911 PE=3 SV=1)

HSP 1 Score: 835.1 bits (2156), Expect = 1.7e-238
Identity = 420/542 (77.49%), Postives = 466/542 (85.98%), Query Frame = 0

Query: 2   MGMLLQWRVLLHLFFVALIWFSSSYSLQESFVECFNSTFYYSSARSIPISEVVFTNESAS 61
           MG+L   R+  HLF +     SSS  LQESF+ECFNST Y  S  S+PISEVVFTNESAS
Sbjct: 1   MGILHWGRI--HLFLIFFHCVSSSDCLQESFMECFNSTTY--SKHSVPISEVVFTNESAS 60

Query: 62  FSSLLQFSIRNLRFLNTSSPKPLFLVTPFHESHVQAAVVCAKNNGLQVRVRSGGHDYEGL 121
           FSSLL+ SIRNLRFL  SSPKP FLVTPF  SHVQAAVVCAK   LQVRVRSGGHDYEGL
Sbjct: 61  FSSLLRLSIRNLRFLTNSSPKPQFLVTPFRASHVQAAVVCAKKKSLQVRVRSGGHDYEGL 120

Query: 122 SYVSTDAPFIVIDLNNLRSIDIDIKTKTASVETGATLGELYYRIAEKSPIHGFPAGSCPT 181
           SYVS+  PFIVIDL NL SI IDI+T+TASVETGATLGE+YYRIAEKSPIHGFPAGSCPT
Sbjct: 121 SYVSSQTPFIVIDLINLSSIHIDIETETASVETGATLGEVYYRIAEKSPIHGFPAGSCPT 180

Query: 182 VGVGGHVSGGGFGTLFRKYGLASDNVIDAKIVDFNGRILDRNSMGEDLFWAIRGGGGASF 241
           VGVGGHVSGGGFGTLFRKYGLA+DNVIDAKIVDFNGRI DRNSMGEDLFWAIRGGGGASF
Sbjct: 181 VGVGGHVSGGGFGTLFRKYGLAADNVIDAKIVDFNGRIRDRNSMGEDLFWAIRGGGGASF 240

Query: 242 GVILSWKLKLVYVPSNVTVFLVQRTLEQGAIQLFQKWQTIAHKLDEDLFLHVTAGVIEQN 301
           GVILSWKLKLV VP +VTVF V RTL+QGA  LFQKWQ IAHK+D+DLFLHVT  V++  
Sbjct: 241 GVILSWKLKLVSVPPDVTVFNVHRTLDQGATHLFQKWQKIAHKMDQDLFLHVTTKVVDHK 300

Query: 302 ---KETPNETSKTILLSFDSLFLGPIERLIPLMNSHFPELGLQRNNCTEMSWIQSVLYFA 361
                +   +SKTI LSF SLFLGPIERLIPLMN+HF ELGL+RN+CTEMSWIQSVL+FA
Sbjct: 301 TKLSSSKEASSKTIKLSFTSLFLGPIERLIPLMNTHFHELGLKRNDCTEMSWIQSVLFFA 360

Query: 362 GLSTKSPLEVLLKR-PPLSNIFFKAKSDFVTSPIPQTGLEALWAKMLEEEASFLILTPYG 421
            LST++P E+L++R PP+S+ FF AKSD+VTSPI QTGLEALW+K+LEEE S LILTPYG
Sbjct: 361 DLSTETPPEILMERSPPISDTFFTAKSDYVTSPISQTGLEALWSKLLEEEKSELILTPYG 420

Query: 422 GKMSQISDSETPFPHRRGNIFGIQYMVTWENANETYKHLSWIKELYAYMEPYVSKYPRAA 481
           GKM QIS+SETPFPHRRGNI+GIQY+  WENANET KHLSW++E+Y+YM+P+VSKYPR A
Sbjct: 421 GKMDQISESETPFPHRRGNIYGIQYLAAWENANETEKHLSWMREVYSYMQPFVSKYPRGA 480

Query: 482 YFNYRDLDLGRNYGRNTSYEEAKVWGLKYYKSNFDRLVRVKTKFDPSNFFWNEQSIPPSL 540
           Y NYRDLDLGRNY RNTSY+EAKVWGLKY+K NF+RLVRVKTK DP NFFWNEQSIP   
Sbjct: 481 YLNYRDLDLGRNYERNTSYDEAKVWGLKYFKGNFERLVRVKTKVDPLNFFWNEQSIPLCH 538

BLAST of Spg035789 vs. ExPASy TrEMBL
Match: A0A6J1KH65 (berberine bridge enzyme-like 15 OS=Cucurbita maxima OX=3661 GN=LOC111495709 PE=3 SV=1)

HSP 1 Score: 831.2 bits (2146), Expect = 2.4e-237
Identity = 416/530 (78.49%), Postives = 466/530 (87.92%), Query Frame = 0

Query: 14  LFFVALIWFSS---SYSLQESFVECFNSTFYYSSARSIPISEVVFTNESASFSSLLQFSI 73
           LF++ LIW SS   S++LQ+SFVECF+S   YSS +SIPIS+VVFTNESASFSSLL  SI
Sbjct: 5   LFYLPLIWLSSSSPSHALQQSFVECFSS---YSSPQSIPISKVVFTNESASFSSLLHQSI 64

Query: 74  RNLRFLNTSSPKPLFLVTPFHESHVQAAVVCAKNNGLQVRVRSGGHDYEGLSYVSTDAPF 133
           RNLRFLNTSS KPLFLVTPFHESHVQAAVVCAK  GLQ+RVRSGGHDYEGLSY+S+ + F
Sbjct: 65  RNLRFLNTSS-KPLFLVTPFHESHVQAAVVCAKKKGLQLRVRSGGHDYEGLSYLSSQSQF 124

Query: 134 IVIDLNNLRSIDIDIKTKTASVETGATLGELYYRIAEKSPIHGFPAGSCPTVGVGGHVSG 193
           IV+DL NLRSID+DI+T+TASVETGATLGE YYRIAEKS IHGFPAGSCPTVGVGGH+SG
Sbjct: 125 IVLDLTNLRSIDVDIETETASVETGATLGEFYYRIAEKSLIHGFPAGSCPTVGVGGHISG 184

Query: 194 GGFGTLFRKYGLASDNVIDAKIVDFNGRILDRNSMGEDLFWAIRGGGGASFGVILSWKLK 253
           GGFGTLFRK+GLA+D VIDAKIVDFNGRI+DRN+MGE+LFWAIRGGGGASFGVILSWKLK
Sbjct: 185 GGFGTLFRKHGLAADKVIDAKIVDFNGRIMDRNTMGEELFWAIRGGGGASFGVILSWKLK 244

Query: 254 LVYVPSNVTVFLVQRTLEQGAIQLFQKWQTIAHKLDEDLFLHVTAGVIEQNKETPNETSK 313
           LV +P  +T+F VQ+T +QGA+QLFQKWQ IA+KLDEDLFLHVT GV EQ          
Sbjct: 245 LVSLPPKLTIFHVQKTPKQGAMQLFQKWQRIAYKLDEDLFLHVTIGVQEQ---------- 304

Query: 314 TILLSFDSLFLGPIERLIPLMNSHFPELGLQRNNCTEMSWIQSVLYFAGLSTKSPLEVLL 373
           TI LSF +LFLGPIE+LIPLMN+ FPELGLQR N TEM+WIQS+L+FAG   ++P ++LL
Sbjct: 305 TISLSFVALFLGPIEKLIPLMNTQFPELGLQRENYTEMNWIQSILFFAGFPIQTPPQILL 364

Query: 374 KRPPLSNIFFKAKSDFVTSPIPQTGLEALWAKMLEEEASFLILTPYGGKMSQISDSETPF 433
           K+ PLSN FFKAKSDFVT PIPQ GLE LW K+LEEE S+LILTPYGGKMSQIS+ ETPF
Sbjct: 365 KK-PLSNAFFKAKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGKMSQISELETPF 424

Query: 434 PHRRGNIFGIQYMVTWENANETYKHLSWIKELYAYMEPYVSKYPRAAYFNYRDLDLGRNY 493
           PHR+GNIFGIQYMV+WEN +E+YKHLSWI+E+YAYMEPYVSK PR AY NYRDLDLGRNY
Sbjct: 425 PHRKGNIFGIQYMVSWENGSESYKHLSWIREVYAYMEPYVSKDPRGAYLNYRDLDLGRNY 484

Query: 494 GRNTSYEEAKVWGLKYYKSNFDRLVRVKTKFDPSNFFWNEQSIPPSLYHS 541
           GRN  YEEAKVWGLKYYKSNF+RLVRVKTK DPSNFFWNEQSIP SLY S
Sbjct: 485 GRNVRYEEAKVWGLKYYKSNFERLVRVKTKVDPSNFFWNEQSIPTSLYRS 519

BLAST of Spg035789 vs. ExPASy TrEMBL
Match: A0A6J1EGS9 (berberine bridge enzyme-like 15 OS=Cucurbita moschata OX=3662 GN=LOC111434055 PE=3 SV=1)

HSP 1 Score: 827.0 bits (2135), Expect = 4.5e-236
Identity = 415/530 (78.30%), Postives = 467/530 (88.11%), Query Frame = 0

Query: 14  LFFVALIWFSS---SYSLQESFVECFNSTFYYSSARSIPISEVVFTNESASFSSLLQFSI 73
           LF++ LIW SS   S++LQ+SFVECF+S   YSS +SIPIS+VVFTNESASFSSLLQ SI
Sbjct: 5   LFYLPLIWLSSSSPSHALQQSFVECFSS---YSSPQSIPISKVVFTNESASFSSLLQQSI 64

Query: 74  RNLRFLNTSSPKPLFLVTPFHESHVQAAVVCAKNNGLQVRVRSGGHDYEGLSYVSTDAPF 133
           RNLRFLNTSS KPLFLVTPFHESHVQAAVVCA+  GLQ+RVRSGGHDYEGLSYVS+ + F
Sbjct: 65  RNLRFLNTSS-KPLFLVTPFHESHVQAAVVCAQKKGLQLRVRSGGHDYEGLSYVSSQSQF 124

Query: 134 IVIDLNNLRSIDIDIKTKTASVETGATLGELYYRIAEKSPIHGFPAGSCPTVGVGGHVSG 193
           I++DL+NL SIDIDI+T+TASVETGATLGELYYRIAEKS IHGFPAGSCPTVGVGGH+SG
Sbjct: 125 ILLDLSNLHSIDIDIETETASVETGATLGELYYRIAEKSLIHGFPAGSCPTVGVGGHISG 184

Query: 194 GGFGTLFRKYGLASDNVIDAKIVDFNGRILDRNSMGEDLFWAIRGGGGASFGVILSWKLK 253
           GGFGTLFRK+GLA+DNVIDAKIVDFNGRI+DRN+MGE+LFWAIRGGGGASFGVILSWKLK
Sbjct: 185 GGFGTLFRKHGLAADNVIDAKIVDFNGRIMDRNTMGEELFWAIRGGGGASFGVILSWKLK 244

Query: 254 LVYVPSNVTVFLVQRTLEQGAIQLFQKWQTIAHKLDEDLFLHVTAGVIEQNKETPNETSK 313
           LV +P  +T+F VQ+T +QGAIQLFQKWQ IA+KLDEDLFLHVT GV EQ          
Sbjct: 245 LVCLPPKLTIFHVQKTPKQGAIQLFQKWQRIAYKLDEDLFLHVTIGVQEQ---------- 304

Query: 314 TILLSFDSLFLGPIERLIPLMNSHFPELGLQRNNCTEMSWIQSVLYFAGLSTKSPLEVLL 373
           TI LSF +LFLGPIE+LIPLMN+ FPEL LQR N TEM+WIQS+L+FAG   ++P ++LL
Sbjct: 305 TISLSFVALFLGPIEKLIPLMNTQFPELVLQRENYTEMNWIQSILFFAGFPIQTPPQILL 364

Query: 374 KRPPLSNIFFKAKSDFVTSPIPQTGLEALWAKMLEEEASFLILTPYGGKMSQISDSETPF 433
           K+  LSN FFKAKSDFVT PIPQ GLE LW K+LEEE S+LILTPYGGKMSQIS+ ETPF
Sbjct: 365 KK-SLSNAFFKAKSDFVTYPIPQNGLEGLWNKVLEEEGSYLILTPYGGKMSQISELETPF 424

Query: 434 PHRRGNIFGIQYMVTWENANETYKHLSWIKELYAYMEPYVSKYPRAAYFNYRDLDLGRNY 493
           PHR+GNIFGIQYMV+WEN +E+YKHLSWI+E+YAYMEPYVSK PR AY NYRDLDLGRNY
Sbjct: 425 PHRKGNIFGIQYMVSWENGSESYKHLSWIREVYAYMEPYVSKDPRGAYLNYRDLDLGRNY 484

Query: 494 GRNTSYEEAKVWGLKYYKSNFDRLVRVKTKFDPSNFFWNEQSIPPSLYHS 541
           GRN  YEEA+VWGLKYY+SNF+RLVRVKTK DP NFFWNEQSIPPSLY S
Sbjct: 485 GRNVRYEEAEVWGLKYYRSNFERLVRVKTKVDPFNFFWNEQSIPPSLYRS 519

BLAST of Spg035789 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 556.2 bits (1432), Expect = 2.9e-158
Identity = 277/531 (52.17%), Postives = 370/531 (69.68%), Query Frame = 0

Query: 14  LFFVALIWFS---SSYSLQESFVECFNSTFYYSSARSIPISEVVFT--NESASFSSLLQF 73
           LF V L+  S   SS +LQ+ FV+C       +S  S PI+   F+    +  F   L+ 
Sbjct: 12  LFLVTLLLISVPLSSSTLQQDFVKCLVD----NSDVSFPITASFFSPDQNATLFKEELES 71

Query: 74  SIRNLRFLNTSSPKPLFLVTPFHESHVQAAVVCAKNNGLQVRVRSGGHDYEGLSYVSTD- 133
           + +NLR+L  S+PKP+F+  P +E+HVQAAVVCAK   L +R+RSGGHDYEGLS+V+ D 
Sbjct: 72  TAQNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDE 131

Query: 134 APFIVIDLNNLRSIDIDIKTKTASVETGATLGELYYRIAEKSPIHGFPAGSCPTVGVGGH 193
            PF+++DL+ LR +D+D+ + +A    GAT+GE+YYRI EKS  HGFPAG C ++G+GGH
Sbjct: 132 TPFVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGH 191

Query: 194 VSGGGFGTLFRKYGLASDNVIDAKIVDFNGRILDRNSMGEDLFWAIRGGGGASFGVILSW 253
           + GG +G++ RK+GL +DNV+DA+IVD NG+ILDR +MGED+FWAIRGGGG SFGVIL+W
Sbjct: 192 LVGGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAW 251

Query: 254 KLKLVYVPSNVTVFLVQRTLEQGAIQLFQKWQTIAHKLDEDLFLHVTAGVIEQNKETPNE 313
           K+KLV VP+ VTVF V +TLEQ   ++  KW+ IA KLD+DLF+ V   +I    +T   
Sbjct: 252 KIKLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRV---IISPASKTTKP 311

Query: 314 TSKTILLSFDSLFLGPIERLIPLMNSHFPELGLQRNNCTEMSWIQSVLYFAGLSTKSPLE 373
            ++TI +S+ + FLG   RL+ +M   FPELGL + +CTEMSWI+SV+Y AG    +  E
Sbjct: 312 GNRTISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPE 371

Query: 374 VLLKRPPLSNIFFKAKSDFVTSPIPQTGLEALWAKMLEEEASFLILTPYGGKMSQISDSE 433
            LL    L    FKAKSDFV  PIP  GLE LW + LEE++   I  PYGG MS+IS+SE
Sbjct: 372 ALLAGKSLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLTIWNPYGGMMSRISESE 431

Query: 434 TPFPHRRGNIFGIQYMVTWENAN-ETYKHLSWIKELYAYMEPYVSKYPRAAYFNYRDLDL 493
            PFPHR G +F IQ++ TW++      +H+ WI+E+Y+YME YVSK PR AY NYRDLDL
Sbjct: 432 IPFPHRNGTLFKIQWLSTWQDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDL 491

Query: 494 GRNYGRNTSYEEAKVWGLKYYKSNFDRLVRVKTKFDPSNFFWNEQSIPPSL 538
           G N G      +A+ WG KYYK NF+RLV++K +FDP NFF +EQS+P  +
Sbjct: 492 GTNEGET----DAREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPTKI 531

BLAST of Spg035789 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 553.5 bits (1425), Expect = 1.9e-157
Identity = 276/533 (51.78%), Postives = 373/533 (69.98%), Query Frame = 0

Query: 15  FFVALIWFSSSY------SLQESFVECFNSTFYYSSARSIPISEVVFTNE--SASFSSLL 74
           F V L+  S SY      ++Q+ FV C       +S  S P+    FT++  ++SF   L
Sbjct: 12  FLVTLLLLSLSYIPLSFSTIQQDFVMCLVD----NSDASFPMDSSFFTHDLNASSFKLAL 71

Query: 75  QFSIRNLRFLNTSSPKPLFLVTPFHESHVQAAVVCAKNNGLQVRVRSGGHDYEGLSYVS- 134
           + S +NLR+L  S+PKP F+  P +E+HVQAAV+CAK   L +R+RSGGHDYEGLSYVS 
Sbjct: 72  ETSAQNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSE 131

Query: 135 TDAPFIVIDLNNLRSIDIDIKTKTASVETGATLGELYYRIAEKSPIHGFPAGSCPTVGVG 194
            +  F+++DL+ LR I +DI++ +A V  GA++GE+YYRI EKS IHGFPAG C ++G+G
Sbjct: 132 METAFVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIG 191

Query: 195 GHVSGGGFGTLFRKYGLASDNVIDAKIVDFNGRILDRNSMGEDLFWAIRGGGGASFGVIL 254
           GH+ GG +G++ RK+GL +DNV+DA+IVD +G+IL+R +MGED+FWAIRGGGG SFGVIL
Sbjct: 192 GHIIGGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVIL 251

Query: 255 SWKLKLVYVPSNVTVFLVQRTLEQGAIQLFQKWQTIAHKLDEDLFLHVTAGVIEQNKETP 314
           +WK+KLV VP  VTVF V RTLEQ   +L  KWQ +A KLDEDLF+ V   +I+   +TP
Sbjct: 252 AWKIKLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRV---IIQPTSKTP 311

Query: 315 NETSKTILLSFDSLFLGPIERLIPLMNSHFPELGLQRNNCTEMSWIQSVLYFAGLSTKSP 374
               +TI  S+   FLG   RL+ +M   FP+LGL + +C E SWI+SV+Y AG  + +P
Sbjct: 312 KSKERTISTSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAP 371

Query: 375 LEVLLKRPPLSNIFFKAKSDFVTSPIPQTGLEALWAKMLEEEASFLILTPYGGKMSQISD 434
            E LL    L   +FKAKSD+V  PIP  GLE LW K+LEE++   I  PYGG M++I +
Sbjct: 372 SEALLDGKSLFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEEDSPLTIWNPYGGMMAKIPE 431

Query: 435 SETPFPHRRGNIFGIQYMVTWENANET-YKHLSWIKELYAYMEPYVSKYPRAAYFNYRDL 494
           +ETPFPHR G +F IQ++  W++   +  KH+ W++E+Y+YME YVSK PR+AY NYRDL
Sbjct: 432 TETPFPHRSGTLFKIQWLTLWQDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDL 491

Query: 495 DLGRNYGRNTSYEEAKVWGLKYYKSNFDRLVRVKTKFDPSNFFWNEQSIPPSL 538
           DLG N G+ +   +A+ WG +Y+K NF+RLV +K KFDP NFF +EQSIP  L
Sbjct: 492 DLGMN-GKGS---DAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIPTEL 533

BLAST of Spg035789 vs. TAIR 10
Match: AT5G44380.1 (FAD-binding Berberine family protein )

HSP 1 Score: 542.0 bits (1395), Expect = 5.6e-154
Identity = 271/535 (50.65%), Postives = 372/535 (69.53%), Query Frame = 0

Query: 11  LLHLFFVALIWFSSSYSLQESFVECFNSTFYYSSARSIPISEVVFT--NESASFSSLLQF 70
           +L+  F  +   SSS S+Q+ F+ C     +     S P+ + +FT     + F+ +L+ 
Sbjct: 17  VLYFSFYCITPTSSSASIQDQFINCVKRNTHV----SFPLEKTLFTPAKNVSLFNQVLES 76

Query: 71  SIRNLRFLNTSSPKPLFLVTPFHESHVQAAVVCAKNNGLQVRVRSGGHDYEGLSYVS-TD 130
           + +NL+FL  S PKP F+  P H+S VQA+++C+K  G+  RVRSGGHD+E LSYVS  +
Sbjct: 77  TAQNLQFLAKSMPKPGFIFRPIHQSQVQASIICSKKLGIHFRVRSGGHDFEALSYVSRIE 136

Query: 131 APFIVIDLNNLRSIDIDIKTKTASVETGATLGELYYRIAEKSPIHGFPAGSCPTVGVGGH 190
            PFI++DL+ L+ I++DI++ +A V+ GATLGELYYRIAEKS IHGFPAG C +VG+GG+
Sbjct: 137 KPFILLDLSKLKQINVDIESNSAWVQPGATLGELYYRIAEKSKIHGFPAGLCTSVGIGGY 196

Query: 191 VSGGGFGTLFRKYGLASDNVIDAKIVDFNGRILDRNSMGEDLFWAIRGGGGASFGVILSW 250
           ++GGG+GTL RKYGLA DNV+D K+VD NG++LDR +MGEDLFWAIRGGGGASFG++L+W
Sbjct: 197 MTGGGYGTLMRKYGLAGDNVLDVKMVDANGKLLDRAAMGEDLFWAIRGGGGASFGIVLAW 256

Query: 251 KLKLVYVPSNVTVFLVQRTLEQGA-IQLFQKWQTIAHKLDEDLFLHVTAGVIEQNKETPN 310
           K+KLV VP  VTVF V +TLEQ A ++   KWQ I+ K+ E++ + V        +   N
Sbjct: 257 KIKLVPVPKTVTVFTVTKTLEQDARLKTISKWQQISSKIIEEIHIRVVL------RAAGN 316

Query: 311 ETSKTILLSFDSLFLGPIERLIPLMNSHFPELGLQRNNCTEMSWIQSVLYFAGLSTKSPL 370
           + +KT+ +++   FLG    L+ +M   FPELGL + +CTEMSWI++ L+  G  T SP+
Sbjct: 317 DGNKTVTMTYLGQFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHGGFPTGSPI 376

Query: 371 EVLLK-RPPLSNIFFKAKSDFVTSPIPQTGLEALWAKMLEEEASFLILTPYGGKMSQISD 430
           E+LL+ + PL   +FKA SDFV  PIP  GL+ ++ +++E   +FL  TPYGG MS+I +
Sbjct: 377 EILLQLKSPLGKDYFKATSDFVKEPIPVIGLKGIFKRLIEGNTTFLNWTPYGGMMSKIPE 436

Query: 431 SETPFPHRRGNIFGIQYMVTW-ENANETYKHLSWIKELYAYMEPYVSKYPRAAYFNYRDL 490
           S  PFPHR G +F I Y   W EN   + + ++WIKE+Y YM PYVS  PR AY NYRDL
Sbjct: 437 SAIPFPHRNGTLFKILYYANWLENDKTSSRKINWIKEIYNYMAPYVSSNPRQAYVNYRDL 496

Query: 491 DLGRNYGR-NTSYEEAKVWGLKYYKSNFDRLVRVKTKFDPSNFFWNEQSIPPSLY 539
           D G+N      ++ EAK+WG KY+K NFDRLV++KTK DP NFF +EQSIPP  Y
Sbjct: 497 DFGQNKNNAKVNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQSIPPMPY 541

BLAST of Spg035789 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 539.3 bits (1388), Expect = 3.6e-153
Identity = 269/533 (50.47%), Postives = 370/533 (69.42%), Query Frame = 0

Query: 6   LQWRVLLHLFFVALIWFSSSYSL-QESFVECFNSTFYYSSARSIPISEVVFTNESASFSS 65
           +++ ++L LFFV  IW SSS S   E+F +C  S    +S    PIS  +F + + S+SS
Sbjct: 1   MKYALILVLFFVVFIWQSSSSSANSETFTQCLTS----NSDPKHPISPAIFFSGNGSYSS 60

Query: 66  LLQFSIRNLRFLNTSSPKPLFLVTPFHESHVQAAVVCAKNNGLQVRVRSGGHDYEGLSYV 125
           +LQ +IRNLRF  TS+PKP  ++   HESHVQAA+ C K + LQ+++RSGGHDY+GLSYV
Sbjct: 61  VLQANIRNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYV 120

Query: 126 S-TDAPFIVIDLNNLRSIDIDIKTKTASVETGATLGELYYRIAEKSPIHGFPAGSCPTVG 185
           + +  PF V+D+ NLRS+D+D+ +KTA V+TGA LGE+YY I EKS    +PAG CPTVG
Sbjct: 121 TYSGKPFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVG 180

Query: 186 VGGHVSGGGFGTLFRKYGLASDNVIDAKIVDFNGRILDRNSMGEDLFWAIRGGGGASFGV 245
           VGGH+SGGG+G + RKYGL  DN IDA++VD NG+ILDR  MGEDL+WAI GGGG S+GV
Sbjct: 181 VGGHISGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGV 240

Query: 246 ILSWKLKLVYVPSNVTVFLVQRTLEQGAIQLFQKWQTIAHKLDEDLFLHVTAGVIEQNKE 305
           +L++K+ LV VP NVTVF + RTLEQ A  +  +WQ +A KL ++LF+     V+     
Sbjct: 241 VLAYKINLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTV- 300

Query: 306 TPNETSKTILLSFDSLFLGPIERLIPLMNSHFPELGLQRNNCTEMSWIQSVLYFAGLSTK 365
               + KT+  +F ++FLG    L+ ++N  FPELGL R++CTE SWIQSVL++  +   
Sbjct: 301 ---SSQKTVRTTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVG 360

Query: 366 SPLEVLLKRPPLSNIFFKAKSDFVTSPIPQTGLEALWAKMLEEEASFLILTPYGGKMSQI 425
           S   +LL+R    N + K KSD+V  PI +TGLE++W KM+E E   +   PYGG+M +I
Sbjct: 361 SSETLLLQRNQPVN-YLKRKSDYVREPISRTGLESIWKKMIELEIPTMAFNPYGGEMGRI 420

Query: 426 SDSETPFPHRRGNIFGIQYMVTWENANETYKHLSWIKELYAYMEPYVSKYPRAAYFNYRD 485
           S + TPFP+R GN++ IQY   W +   T +++   ++LY +M P+VSK PR ++FNYRD
Sbjct: 421 SSTVTPFPYRAGNLWKIQYGANWRDETLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRD 480

Query: 486 LDLGRN--YGRNTSYEEAKVWGLKYYKSNFDRLVRVKTKFDPSNFFWNEQSIP 535
           +DLG N   G+ +SY E K +G KY+  NF+RLV++KT+ D  NFF NEQSIP
Sbjct: 481 VDLGINSHNGKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIP 524

BLAST of Spg035789 vs. TAIR 10
Match: AT5G44390.1 (FAD-binding Berberine family protein )

HSP 1 Score: 539.3 bits (1388), Expect = 3.6e-153
Identity = 273/524 (52.10%), Postives = 368/524 (70.23%), Query Frame = 0

Query: 20  IWFSSSYSLQESFVECFNSTFYYSSARSIPISEVVFTNE--SASFSSLLQFSIRNLRFLN 79
           I  +SS SLQ+ F++C     Y ++     + +  FT E  ++ F+ +L+ + +N R+L 
Sbjct: 25  ITLTSSTSLQDDFIKC----LYRNTNVRFTLDKTFFTPERNASIFTEVLESTAQNQRYLT 84

Query: 80  TSSPKPLFLVTPFHESHVQAAVVCAKNNGLQVRVRSGGHDYEGLSYVS-TDAPFIVIDLN 139
            + PKP F+  P HESHVQA+V+C+K   +  RVRSGGHDYEG+SYVS  + PF++IDL+
Sbjct: 85  KTMPKPGFIFKPVHESHVQASVICSKKLEIHFRVRSGGHDYEGVSYVSQIEKPFVLIDLS 144

Query: 140 NLRSIDIDIKTKTASVETGATLGELYYRIAEKSPIHGFPAGSCPTVGVGGHVSGGGFGTL 199
            LR I++DIK  +A VE GAT+GELYYRIAEKS  HGFPAG  P++G+GGH++GG +G+L
Sbjct: 145 KLRQINVDIKDTSAWVEAGATVGELYYRIAEKSKFHGFPAGVYPSLGIGGHITGGAYGSL 204

Query: 200 FRKYGLASDNVIDAKIVDFNGRILDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVYVPS 259
            RKYGLA+DNV+DAKIVD NG++LDR SMGEDLFWAIRGG G SFG+ILSWK+KLV VP 
Sbjct: 205 MRKYGLAADNVLDAKIVDANGKLLDRASMGEDLFWAIRGGSGGSFGIILSWKIKLVPVPE 264

Query: 260 NVTVFLVQRTLEQG-AIQLFQKWQTIAHKLDEDLFLHVTAGVIEQNKETPNETSKTILLS 319
            +TVF V +T EQ  + ++  KWQ IA  L ++LFL V   V      + N+ +KT+ ++
Sbjct: 265 TLTVFTVTKTFEQDRSFKILSKWQEIADNLVDELFLRVFFTV------SGNKANKTVTMA 324

Query: 320 FDSLFLGPIERLIPLMNSHFPELGLQRNNCTEMSWIQSVLYFAGLSTK--SPLEVLLK-R 379
           +   FLG    L+ +M   FPELGL + +C EMSWI S++Y +G  T    P+E+LL+ +
Sbjct: 325 YIGQFLGEKGTLMEVMKKDFPELGLTQKDCIEMSWIDSIIYNSGFPTNPPPPIEILLQAK 384

Query: 380 PPLSNIFFKAKSDFVTSPIPQTGLEALWAKMLEEEASFLILTPYGGKMSQISDSETPFPH 439
            P+  ++FK KSDF   PIP  GLE ++ K+LEE+A+ +I TPYGGKM +I +SE PFPH
Sbjct: 385 SPIGKVYFKGKSDFAKKPIPVLGLEGMFKKLLEEDAALVIWTPYGGKMDKIPESEIPFPH 444

Query: 440 RRGNIFGIQYMVTWENANE-TYKHLSWIKELYAYMEPYVSKYPRAAYFNYRDLDLGRNYG 499
           R G  F IQY  +W ++ +   +   WI+ELY YM PYVS  PR AY NYRDLDLG+N  
Sbjct: 445 RNGTNFMIQYYRSWSDSEKRPNRRTKWIRELYGYMTPYVSSNPRQAYVNYRDLDLGQNKD 504

Query: 500 RNTS-YEEAKVWGLKYYKSNFDRLVRVKTKFDPSNFFWNEQSIP 535
            + S + EAK+WG  Y+K NF+RLVR+K+K DP NFF +EQSIP
Sbjct: 505 NSKSNFIEAKIWGANYFKDNFNRLVRIKSKVDPDNFFRHEQSIP 538