Spg034543 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg034543
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionAluminum-activated malate transporter
Locationscaffold4: 12850579 .. 12854745 (-)
RNA-Seq ExpressionSpg034543
SyntenySpg034543
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCCATTTGAGCATTCAAGGAGTGTAAACACTTTGGTCCTCTCTTCTCTTCCATTGCCAGACAGAATTTTGAAGCCTTTGAGGTGCAAAAAAGCAAAGCACATAGAAAAAGTTGATCTTGATTATTTGTTGTTGTATTGAAAACAATGGCAAAAGCAGGAAATTTGGAATGGAGGGTGAATGTGGGTGATGGGACAAGAGAGATTTTAAGGCCAGATGTCCCATTTGTGCAGAAAATGGGGATGAGGGTGAAGGGTTTTATTTGGGGATTGTTTGTGAAGGCTTGGAAGTTCTTGGAAAAGGCTTGGGGAATAGCAAATTCTGAGCCTAAGAAGGCTGTTCATGGGCTTAAAGTGGGGCTGGCTCTGACCCTTGTGTCCATCTTTTACTACATGAGACCTTTGTATGAAGGAGTTGGAGGGAATGCCATGTGGGCCATTATGACTGTTGTTGTCACTTTTGAATCCACTGTGGGTTAGTTTTCCCAAACCCCTTTCTCCTCCTTATAAGCTTATCAAATTTTATCCCTTCTTTTCAACTCATGATATATTAATAGTTTACATGTGTTAGTCTTCGATGAATCAATTTGGAAATTATTTTAACTGTTTCTAGGAAGTCTTTTAGGGGATGTTTGGGACTCGGGATTAGAATGGAATGGATTGCATTGGAATCTCCAATGTTTGTTGGGCCGGATTTAAATTGGAATGAGACCACTTTTTGGATTCCAATGTGAATTCTTACCATGGAAGGGTTGGAATCCACCATTCTAATCCATTCCAACACTTTTTTATAAAGTTGGTGGGACTCGTCATTTCTCTTTCTATTTTCCTTATAATTTTGGTGGACCCCACTATTTTTCTCTTCATTTTTTTTTTCATAATTTTGATGGTTCTCATCATTATTGTCTCCATTTTCTCATAATTTATTTCTTCATTAATATATATTTAATTAATAATAATAGAATATTCTTTAAAAAATAATAAAAATAACAATAGTAGAATATATATAAAATTTTATAATTTTAAGATAAGAAAAGTATATTTTAGATGATGGTTAAAATATCATCTTGATCCCTATATTTTTTATTTTGTTTTCTTTTAGTCCCTATATTTTCAAATGTCTAATTTTAGTGATCGTACAAGCTTAAAATTTCGCCCTATAATGAGTTTATTGTTAAACTATCTTAAAAAAAAATAGACTAAGTGTAACAATTTTCTTGTTTCTTTAAACATCATATTGAGAAATGATGAACTGATGTCTCAAGAAATAGGGATAAATCATAGAAATTGAAAGCAAACCCTGAATTATGAAGATTAAAATGATGATTTAACCAAACATTTTAAATACATATAACAATGGATTAAGTTTTTGAGTTTAATAGTAATTTAATTGTGGGTGTTTTAACGCGGGAAAGTTATTGCTCCACTTTGCAGGCGCAACGTTTTACAAATGTGTAAATAGAGTCATAGGAACATCTCTTGCTGGGTGTTTGGGCATTGGAGTTCATTGGGTTGCAGCTGAATCTGGCGATAGATTTGAGCCAATTATTCTTGGGATCTCACTTTTTCTTCTAGGTAAACCATTCTTACAACATTTTGGGTCTCCAAAACTTTTACGAATATTTCATGACAACAACTCTCATATTGGCTCAATGTTTTCATGCATGCAGCTTCAGCAGCCACCTTCTCTCGGTTCATTCCATCAGTGAAGACGAGATTCGACTATGGCACCATGATCTTCATCCTCACCTTCTGTTTAGTTTCGATCTCTGGTTATCGAGTCGATAAGCTGTTCGAGCTGGCCCGTACTCGGCTCAGCACCATCGCCATTGGGACATCGCTGTGTATTTTCGTCAGCATGCTTTTCTGCCCCATGTGGGCTGGCAGTCAGCTTCATAGCCTCATTTCCCGCAACTTGGATAAACTTGCCCATGCCTTGGATGGTAAATTCCTATTCCTCCCTTCTTCATTCATATATATTTTGCCCGGGGTCGAGTTAGAAATTTGTTTATAACGAGCAACTTCTAACTTTTTATACATAGTATTTCAATTTTGAAACTTTATGGTTGAAAATTAAAGGAAAAATTTTTAAATCTTCAATAGTTTAGTACGATTTTTAAAATTAAAATAATTAAACTAGAAGGAAAATTGCCTTTTATCCTTCTTTCTTCGCTTTGCCCTTTCGTCTATTTATTAACTCATATATATGTATATATATTTACCTCGGTCATAATCTTCTTCAATTCAGGTTGTGTTTCTGAGTACTTCAAAAACGATGATGATCCAGAGATTAAAAATGAAGATCAGGAGGGTCATGATTCCAAAGTTGAAGGCTATAAGTGTGTGCTTAATTCAAAGGCATCAGAAGAATCTATGGTAAACAAATAGAGACTCTCTTTTAATTCCTTCTCCATACCCTATAAATGTTATTAAAAACTAAGAAATATCATCATGATGCCTTGCCAAAGTGCGACATGTAGTCTCGAATAAGAGGTCAGAAGTTCAAATCCACCCATCCCTAATTGTTGCGTTGAACTCAAAAAAAGAAAAAAAAATCATCATGATGCCTTAATATGGTACCAAAACAAGATATCTTCAATTGATACCAATAGCTTAGGTATTGAGCTATATAAAGAAATTAATGGCATGTTTAGAAGTGGTTTTAAAATGGTTAAAATCATTTTGTCATGTGCATTTAATCATTTAAAATCAATTTTAATGGTAGGTAAATTCACTTCAAAAGTGTAAAATCAAACATTAGAATGATTTTGAATTATTAAATACATGTTTTGGAGTGATTTTGAATATAACAAAAGTGATATTAAATTTGTGAGCTCATACAAGTGCTCAAACAATATATCCAAGATTAAGTACACATAGACCTATCAATTTAAGATTCTACAACAAGGATTTCAAAGATTAAATATTTGAACCAACACATATCATAACTTATTATTCCAAGTTTTTGGGTTGAATAATAGTTTAATAACAATTATGATTTCAAGATTTAACGTATCATAATCTAACTTATCATTTTAAAGCTACTTTTTCTGGATTTCTTAAGCTTTTAGCTCTAACAAACGTGGTACTTTTGTAACTTGCAGGCGAACTTTGCAAGATGGGAACCTGCACATGGGCGTTTTAGTTTCAGACACCCATGGAAAAAATATCTTGAGATTGGAGTAGTAATGCGGAAATGTGCATATTGCATTGAGGCTCTTCATGGCTGCCTAAACTCAGAGATTAAGGTCAATTTTCTAATATAAATCCTATCTTTTTATGAGCCCATTTTTCAAAATAATCAAACTTACCTCATAACCGATCTAGAAGAATCTTCGGACTGATCATCACTAAAATTTTGATCGACATCTATTGAAAAGAAAAAAAATTCTCTTCTGATCTCTTCCTTTTTGCGTTCTTCTTCTTACTTGTCAAAAACATAATTGTAGTCAACAGAAGATTCCAATTGACCAATTAAGGGTAAATTTTGTCACATTTGATCTAATTCTCTTGCTAATTTTGGACACTCATGCAGGCACCAGACTCTTTAAAAATGCATTTGGCTGAACCATGCAAGGCACTGAGCAGCTCTTCATCCAAAGTCTTGAAGGAACTATCCGTTGTGATCAAGAAGATGAAAAAATCGACAAAGATAGACTTGGAAGTATCAGATATGAACATTGCTGTGCAAGAACTTCAAAATGCCATAAAGTCACTTCCAAGCCCTCATATGGGAGTCTCATCAGCAGAGCCAGAGGGGGCCAATCAAGATCCTAAACCAACAAATCCATCTCTAATGGAGCTTCTTCCACTGGCCACATTGGTGTCACTATTGATAGAAACTGTGTCGAGAATCGAACATGTCGTTAATGCAGTCGAAGCGTTAGCGGACGTTGCTAATTTCGATACCGAAGAGGAGAAGAAGAAGCCACCTTCAGATAACCATAACCATCATGTAGCCATGAGGGTCTTTCCAGAGGCCTTAGTTCATTAGATCATCAAAATGGTTGGTTATATCATGACTTGTAAACTGAAATCTCCCTTTCTACTTCAGAAACCATAGTCATATTGCCTTTATGGATACAACTTATTTAGAATTCTGGAGTGTTTTTTCCCCTTTTTTGGGAAATACCATGATGACTACACTGTATAGACTCAAATACTAATATG

mRNA sequence

ATGGCAAAAGCAGGAAATTTGGAATGGAGGGTGAATGTGGGTGATGGGACAAGAGAGATTTTAAGGCCAGATGTCCCATTTGTGCAGAAAATGGGGATGAGGGTGAAGGGTTTTATTTGGGGATTGTTTGTGAAGGCTTGGAAGTTCTTGGAAAAGGCTTGGGGAATAGCAAATTCTGAGCCTAAGAAGGCTGTTCATGGGCTTAAAGTGGGGCTGGCTCTGACCCTTGTGTCCATCTTTTACTACATGAGACCTTTGTATGAAGGAGTTGGAGGGAATGCCATGTGGGCCATTATGACTGTTGTTGTCACTTTTGAATCCACTGTGGGCGCAACGTTTTACAAATGTGTAAATAGAGTCATAGGAACATCTCTTGCTGGGTGTTTGGGCATTGGAGTTCATTGGGTTGCAGCTGAATCTGGCGATAGATTTGAGCCAATTATTCTTGGGATCTCACTTTTTCTTCTAGCTTCAGCAGCCACCTTCTCTCGGTTCATTCCATCAGTGAAGACGAGATTCGACTATGGCACCATGATCTTCATCCTCACCTTCTGTTTAGTTTCGATCTCTGGTTATCGAGTCGATAAGCTGTTCGAGCTGGCCCGTACTCGGCTCAGCACCATCGCCATTGGGACATCGCTGTGTATTTTCGTCAGCATGCTTTTCTGCCCCATGTGGGCTGGCAGTCAGCTTCATAGCCTCATTTCCCGCAACTTGGATAAACTTGCCCATGCCTTGGATGGTTGTGTTTCTGAGTACTTCAAAAACGATGATGATCCAGAGATTAAAAATGAAGATCAGGAGGGTCATGATTCCAAAGTTGAAGGCTATAAGTGTGTGCTTAATTCAAAGGCATCAGAAGAATCTATGGCGAACTTTGCAAGATGGGAACCTGCACATGGGCGTTTTAGTTTCAGACACCCATGGAAAAAATATCTTGAGATTGGAGTAGTAATGCGGAAATGTGCATATTGCATTGAGGCTCTTCATGGCTGCCTAAACTCAGAGATTAAGGCACCAGACTCTTTAAAAATGCATTTGGCTGAACCATGCAAGGCACTGAGCAGCTCTTCATCCAAAGTCTTGAAGGAACTATCCGTTGTGATCAAGAAGATGAAAAAATCGACAAAGATAGACTTGGAAGTATCAGATATGAACATTGCTGTGCAAGAACTTCAAAATGCCATAAAGTCACTTCCAAGCCCTCATATGGGAGTCTCATCAGCAGAGCCAGAGGGGGCCAATCAAGATCCTAAACCAACAAATCCATCTCTAATGGAGCTTCTTCCACTGGCCACATTGGTGTCACTATTGATAGAAACTGTGTCGAGAATCGAACATGTCGTTAATGCAGTCGAAGCGTTAGCGGACGTTGCTAATTTCGATACCGAAGAGGAGAAGAAGAAGCCACCTTCAGATAACCATAACCATCATGTAGCCATGAGGGTCTTTCCAGAGGCCTTAGTTCATTAG

Coding sequence (CDS)

ATGGCAAAAGCAGGAAATTTGGAATGGAGGGTGAATGTGGGTGATGGGACAAGAGAGATTTTAAGGCCAGATGTCCCATTTGTGCAGAAAATGGGGATGAGGGTGAAGGGTTTTATTTGGGGATTGTTTGTGAAGGCTTGGAAGTTCTTGGAAAAGGCTTGGGGAATAGCAAATTCTGAGCCTAAGAAGGCTGTTCATGGGCTTAAAGTGGGGCTGGCTCTGACCCTTGTGTCCATCTTTTACTACATGAGACCTTTGTATGAAGGAGTTGGAGGGAATGCCATGTGGGCCATTATGACTGTTGTTGTCACTTTTGAATCCACTGTGGGCGCAACGTTTTACAAATGTGTAAATAGAGTCATAGGAACATCTCTTGCTGGGTGTTTGGGCATTGGAGTTCATTGGGTTGCAGCTGAATCTGGCGATAGATTTGAGCCAATTATTCTTGGGATCTCACTTTTTCTTCTAGCTTCAGCAGCCACCTTCTCTCGGTTCATTCCATCAGTGAAGACGAGATTCGACTATGGCACCATGATCTTCATCCTCACCTTCTGTTTAGTTTCGATCTCTGGTTATCGAGTCGATAAGCTGTTCGAGCTGGCCCGTACTCGGCTCAGCACCATCGCCATTGGGACATCGCTGTGTATTTTCGTCAGCATGCTTTTCTGCCCCATGTGGGCTGGCAGTCAGCTTCATAGCCTCATTTCCCGCAACTTGGATAAACTTGCCCATGCCTTGGATGGTTGTGTTTCTGAGTACTTCAAAAACGATGATGATCCAGAGATTAAAAATGAAGATCAGGAGGGTCATGATTCCAAAGTTGAAGGCTATAAGTGTGTGCTTAATTCAAAGGCATCAGAAGAATCTATGGCGAACTTTGCAAGATGGGAACCTGCACATGGGCGTTTTAGTTTCAGACACCCATGGAAAAAATATCTTGAGATTGGAGTAGTAATGCGGAAATGTGCATATTGCATTGAGGCTCTTCATGGCTGCCTAAACTCAGAGATTAAGGCACCAGACTCTTTAAAAATGCATTTGGCTGAACCATGCAAGGCACTGAGCAGCTCTTCATCCAAAGTCTTGAAGGAACTATCCGTTGTGATCAAGAAGATGAAAAAATCGACAAAGATAGACTTGGAAGTATCAGATATGAACATTGCTGTGCAAGAACTTCAAAATGCCATAAAGTCACTTCCAAGCCCTCATATGGGAGTCTCATCAGCAGAGCCAGAGGGGGCCAATCAAGATCCTAAACCAACAAATCCATCTCTAATGGAGCTTCTTCCACTGGCCACATTGGTGTCACTATTGATAGAAACTGTGTCGAGAATCGAACATGTCGTTAATGCAGTCGAAGCGTTAGCGGACGTTGCTAATTTCGATACCGAAGAGGAGAAGAAGAAGCCACCTTCAGATAACCATAACCATCATGTAGCCATGAGGGTCTTTCCAGAGGCCTTAGTTCATTAG

Protein sequence

MAKAGNLEWRVNVGDGTREILRPDVPFVQKMGMRVKGFIWGLFVKAWKFLEKAWGIANSEPKKAVHGLKVGLALTLVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNRVIGTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTRFDYGTMIFILTFCLVSISGYRVDKLFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLHSLISRNLDKLAHALDGCVSEYFKNDDDPEIKNEDQEGHDSKVEGYKCVLNSKASEESMANFARWEPAHGRFSFRHPWKKYLEIGVVMRKCAYCIEALHGCLNSEIKAPDSLKMHLAEPCKALSSSSSKVLKELSVVIKKMKKSTKIDLEVSDMNIAVQELQNAIKSLPSPHMGVSSAEPEGANQDPKPTNPSLMELLPLATLVSLLIETVSRIEHVVNAVEALADVANFDTEEEKKKPPSDNHNHHVAMRVFPEALVH
Homology
BLAST of Spg034543 vs. NCBI nr
Match: XP_038906852.1 (aluminum-activated malate transporter 10 [Benincasa hispida])

HSP 1 Score: 812.0 bits (2096), Expect = 2.8e-231
Identity = 411/488 (84.22%), Postives = 446/488 (91.39%), Query Frame = 0

Query: 1   MAKAGNLEWRVNVGDGTREILRPDVPFVQKMGMRVKGFIWG-LFVKAWKFLEKAWGIANS 60
           MA +GNLEWRVN+GDGT EILRP+   + K+ +R+KGFIWG LFVK WKFLEKAWGIANS
Sbjct: 1   MASSGNLEWRVNMGDGTTEILRPEASILGKIMLRIKGFIWGALFVKPWKFLEKAWGIANS 60

Query: 61  EPKKAVHGLKVGLALTLVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNR 120
           EPKKA+HGLKVGLALT+VSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNR
Sbjct: 61  EPKKAIHGLKVGLALTIVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNR 120

Query: 121 VIGTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTRFDYGTMI 180
           VIGTSLAGCLGIGVHW+AAESGD+FEPIILGISLFLLASA TFSRFIPSVK+RFDYG MI
Sbjct: 121 VIGTSLAGCLGIGVHWIAAESGDKFEPIILGISLFLLASATTFSRFIPSVKSRFDYGAMI 180

Query: 181 FILTFCLVSISGYRVDKLFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLHSLISRNL 240
           F+LTFCLVSIS YRV+KLFELARTR+STIAIGTSLCIFVSMLFCP+WAGSQL +L +RNL
Sbjct: 181 FVLTFCLVSISSYRVNKLFELARTRISTIAIGTSLCIFVSMLFCPIWAGSQLQTLTARNL 240

Query: 241 DKLAHALDGCVSEYFKNDDDPEIKNEDQEGHDSKVEGYKCVLNSKASEESMANFARWEPA 300
           DKLAH+LDGCVSEYF+NDD PE+KNE+QE H SKVEGYKCVLNSKASEESMANFARWEPA
Sbjct: 241 DKLAHSLDGCVSEYFENDDTPEMKNEEQENHSSKVEGYKCVLNSKASEESMANFARWEPA 300

Query: 301 HGRFSFRHPWKKYLEIGVVMRKCAYCIEALHGCLNSEIKAPDSLKMHLAEPCKALSSSSS 360
           HGRF FRHPWKKYLE+G VMRK AYCIEALHGCLNSEI+AP+SLK+HLAEPCK LSSSSS
Sbjct: 301 HGRFGFRHPWKKYLEVGGVMRKSAYCIEALHGCLNSEIQAPNSLKLHLAEPCKELSSSSS 360

Query: 361 KVLKELSVVIKKMKKSTKIDLEVSDMNIAVQELQNAIKSLPSPHMGVSSAEPEGANQDPK 420
           KVLKELS+VIKKMKKSTKID  VSDMNIA+QELQNAIKSL  P M VS +E E  N +  
Sbjct: 361 KVLKELSIVIKKMKKSTKIDFLVSDMNIALQELQNAIKSL--PQMEVSLSEQE-ENNEKA 420

Query: 421 PTNPSLMELLPLATLVSLLIETVSRIEHVVNAVEALADVANFDTEEEKKKPPSDNHNHHV 480
              P LMELLPLATLVSLLIET SRIEHVVNAVE LA+VANFD+EEEKKKP  DNH+H+V
Sbjct: 421 TITPPLMELLPLATLVSLLIETTSRIEHVVNAVETLANVANFDSEEEKKKPSLDNHDHNV 480

Query: 481 AMRVFPEA 488
           AMRVFP+A
Sbjct: 481 AMRVFPQA 485

BLAST of Spg034543 vs. NCBI nr
Match: XP_011658401.1 (aluminum-activated malate transporter 10 [Cucumis sativus] >KGN46529.1 hypothetical protein Csa_005000 [Cucumis sativus])

HSP 1 Score: 796.2 bits (2055), Expect = 1.6e-226
Identity = 409/496 (82.46%), Postives = 447/496 (90.12%), Query Frame = 0

Query: 1   MAKAGNLEWRVNVGDGTREILRPDVPFVQKMGMRVKGFIW-GLFVKAWKFLEKAWGIANS 60
           M   GNLEWRVN+GDGT EILRP+  F++ + MR+K FIW GL +KAWKFLEKAWGIANS
Sbjct: 1   MTSNGNLEWRVNMGDGTTEILRPEASFMKNIMMRIKNFIWGGLLLKAWKFLEKAWGIANS 60

Query: 61  EPKKAVHGLKVGLALTLVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNR 120
           EPKKAVHGLKVGLALT+VSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNR
Sbjct: 61  EPKKAVHGLKVGLALTIVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNR 120

Query: 121 VIGTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTRFDYGTMI 180
           VIGTSLAGCLGIGVHW+AAESGD+FEPIILGISLFLLAS  TFSRFIPSVK+RFDYG MI
Sbjct: 121 VIGTSLAGCLGIGVHWIAAESGDKFEPIILGISLFLLASVTTFSRFIPSVKSRFDYGAMI 180

Query: 181 FILTFCLVSISGYRVDKLFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLHSLISRNL 240
           F+LTFCLVSISGYRV+KLFELARTR+STIAIGTSLCIFVSMLFCP+WAGSQL SL +RNL
Sbjct: 181 FVLTFCLVSISGYRVEKLFELARTRISTIAIGTSLCIFVSMLFCPIWAGSQLQSLTARNL 240

Query: 241 DKLAHALDGCVSEYFKNDDDPE--IKNEDQEGHDSKVEGYKCVLNSKASEESMANFARWE 300
           DKLAH+LDGCVSEYF+NDDD E    NED++ ++SKVEGYKCVLNSKASEESMANFARWE
Sbjct: 241 DKLAHSLDGCVSEYFENDDDTEEMKNNEDEKNNNSKVEGYKCVLNSKASEESMANFARWE 300

Query: 301 PAHGRFSFRHPWKKYLEIGVVMRKCAYCIEALHGCLNSEIKAPDSLKMHLAEPCKALSSS 360
           PAHGRF FRHPWKKYLE+G VMRK AYCIEALHGCLNSEI+AP+SLK+HLAEPCKALSSS
Sbjct: 301 PAHGRFGFRHPWKKYLEVGGVMRKSAYCIEALHGCLNSEIQAPNSLKLHLAEPCKALSSS 360

Query: 361 SSKVLKELSVVIKKMKKSTKIDLEVSDMNIAVQELQNAIKSLPSPHMGV--SSAEPEGAN 420
           SS+VLKELS+VIKKMKKSTKID  VS+MN+AVQELQNAIKS PS  M V  S  E E  N
Sbjct: 361 SSEVLKELSIVIKKMKKSTKIDFLVSNMNVAVQELQNAIKSFPSTQMEVSLSEQEEEANN 420

Query: 421 QDPK---PTNPSLMELLPLATLVSLLIETVSRIEHVVNAVEALADVANFDTEEEKKKP-P 480
           +D K    T P LM+LLPLATLVSLLIET SRIEHVVNAVE LA+VAN+D+E+EKKKP  
Sbjct: 421 EDHKAATTTIPPLMKLLPLATLVSLLIETTSRIEHVVNAVETLANVANYDSEDEKKKPSS 480

Query: 481 SDNHNHHVAMRVFPEA 488
           SDNH+H+VAMRVFPEA
Sbjct: 481 SDNHDHNVAMRVFPEA 496

BLAST of Spg034543 vs. NCBI nr
Match: XP_008459754.1 (PREDICTED: aluminum-activated malate transporter 10 [Cucumis melo])

HSP 1 Score: 793.1 bits (2047), Expect = 1.4e-225
Identity = 411/498 (82.53%), Postives = 448/498 (89.96%), Query Frame = 0

Query: 1   MAKAGNLEWRVNVGDGTREILRPDVPFVQKMGMRVKGFIW-GLFVKAWKFLEKAWGIANS 60
           MA  GNLEWRVN+GDGT EILRP   F++ + MR+K FIW GL +KAWKFLEKAWGIANS
Sbjct: 1   MASNGNLEWRVNMGDGTTEILRPQASFMKNIMMRIKDFIWGGLLLKAWKFLEKAWGIANS 60

Query: 61  EPKKAVHGLKVGLALTLVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNR 120
           EPKK VHGLKVGLALT+VSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNR
Sbjct: 61  EPKKVVHGLKVGLALTIVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNR 120

Query: 121 VIGTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTRFDYGTMI 180
           VIGTSLAGCLGIGVHW+AAESGD+FEPIILGISLFLLASAATFSRFIPSVK+RFDYG MI
Sbjct: 121 VIGTSLAGCLGIGVHWIAAESGDKFEPIILGISLFLLASAATFSRFIPSVKSRFDYGAMI 180

Query: 181 FILTFCLVSISGYRVDKLFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLHSLISRNL 240
           F+LTFCLVSISGYRV+KL ELARTR+STIAIGTSLCIFVSMLFCP+WAGSQL SL +RNL
Sbjct: 181 FVLTFCLVSISGYRVEKLLELARTRISTIAIGTSLCIFVSMLFCPIWAGSQLQSLTARNL 240

Query: 241 DKLAHALDGCVSEYFKNDDDP-EIKNEDQEGHDSKVEGYKCVLNSKASEESMANFARWEP 300
           DKLA +LDGCV EYF+NDDD  E+KNEDQ+ ++SKVEGYKCVLNSKASEESMANFARWEP
Sbjct: 241 DKLALSLDGCVCEYFENDDDTGEMKNEDQKNNNSKVEGYKCVLNSKASEESMANFARWEP 300

Query: 301 AHGRFSFRHPWKKYLEIGVVMRKCAYCIEALHGCLNSEIKAPDSLKMHLAEPCKALSSSS 360
           AHGRF FRHPWKKYLE+G VMRK AYCIEALHGCLNSEI+AP+SLK+HLAEPCKALSSSS
Sbjct: 301 AHGRFGFRHPWKKYLEVGGVMRKSAYCIEALHGCLNSEIQAPNSLKLHLAEPCKALSSSS 360

Query: 361 SKVLKELSVVIKKMKKSTKIDLEVSDMNIAVQELQNAIKSLPSPHMGVSSAE---PEGAN 420
           S+VLKELS+VIKKMKKSTKID  VS+MNIAVQELQNAIKSLPS  M VS +E    E  N
Sbjct: 361 SQVLKELSIVIKKMKKSTKIDFLVSNMNIAVQELQNAIKSLPSTQMEVSLSEQDHQEANN 420

Query: 421 QD-----PKPTNPSLMELLPLATLVSLLIETVSRIEHVVNAVEALADVANFDTEEEKKKP 480
           +D        T P LMELLPLATLVSLLIET SRIEHVVNAVE LA+VA++D+E+EKKKP
Sbjct: 421 EDHHKATTAATIPPLMELLPLATLVSLLIETTSRIEHVVNAVETLANVADYDSEDEKKKP 480

Query: 481 -PSDNHNHHVAMRVFPEA 488
             SDNH+H+VAMRVFPEA
Sbjct: 481 SSSDNHDHNVAMRVFPEA 498

BLAST of Spg034543 vs. NCBI nr
Match: XP_022960200.1 (aluminum-activated malate transporter 10 [Cucurbita moschata])

HSP 1 Score: 791.2 bits (2042), Expect = 5.1e-225
Identity = 404/487 (82.96%), Postives = 433/487 (88.91%), Query Frame = 0

Query: 1   MAKAGNLEWRVNVGDGTREILRPDVPFVQKMGMRVKGFIWGLFVKAWKFLEKAWGIANSE 60
           MA  G+LEWRVNVGDG  E+LRP+  FV +M M VKGF+WGLFVK W FLEKAWGIANSE
Sbjct: 1   MASGGSLEWRVNVGDGATEVLRPEGSFVGRMVMGVKGFVWGLFVKGWMFLEKAWGIANSE 60

Query: 61  PKKAVHGLKVGLALTLVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNRV 120
           PKKAVHGLKVGLAL++VS+FYYMRPLYEG+GGNAMWAIMTVVVTFESTVGATFYKCVNRV
Sbjct: 61  PKKAVHGLKVGLALSIVSVFYYMRPLYEGIGGNAMWAIMTVVVTFESTVGATFYKCVNRV 120

Query: 121 IGTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTRFDYGTMIF 180
           IGTSLAG LGIGVHWVAAESGDRFEPIILGISLFLLASA TFSRFIPS+KT+FDYG MIF
Sbjct: 121 IGTSLAGGLGIGVHWVAAESGDRFEPIILGISLFLLASATTFSRFIPSIKTKFDYGAMIF 180

Query: 181 ILTFCLVSISGYRVDKLFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLHSLISRNLD 240
           ILTFCLVSIS YRVDKLFELA TRL+TIAIGTSLCI VSMLFCPMWAGSQLHSL+ RNLD
Sbjct: 181 ILTFCLVSISSYRVDKLFELAHTRLTTIAIGTSLCIIVSMLFCPMWAGSQLHSLVFRNLD 240

Query: 241 KLAHALDGCVSEYFKNDDDPEIKNEDQEGHDSKVEGYKCVLNSKASEESMANFARWEPAH 300
           KLA  LDGCVSEYFKN DDPE++NE+QE HDSK + YKCVLNSKASEESMANFARWEPAH
Sbjct: 241 KLADGLDGCVSEYFKNVDDPEMENENQECHDSKAQSYKCVLNSKASEESMANFARWEPAH 300

Query: 301 GRFSFRHPWKKYLEIGVVMRKCAYCIEALHGCLNSEIKAPDSLKMHLAEPCKALSSSSSK 360
           GRF FRHPWKKYLE+GV MRKCAYCIEALH CLNSEIKAP+SLK+ L EPCKALS+SSSK
Sbjct: 301 GRFGFRHPWKKYLEVGVAMRKCAYCIEALHDCLNSEIKAPNSLKLLLVEPCKALSTSSSK 360

Query: 361 VLKELSVVIKKMKKSTKIDLEVSDMNIAVQELQNAIKSLPSPHMGVSSAEPEGANQDPKP 420
           VLKELS+VIK MKKSTKIDLEVSDMNIAVQELQ AI S PS     ++ E E A      
Sbjct: 361 VLKELSIVIKTMKKSTKIDLEVSDMNIAVQELQAAIASFPSTQ--TANEEHEAA------ 420

Query: 421 TNPSLMELLPLATLVSLLIETVSRIEHVVNAVEALADVANFDTEEEKKKPPSDNHNHHVA 480
           T P LMELLPLATLVSLLIET SRIEHVVNAVE LA+VANFD+EEEKK+   DN++HHVA
Sbjct: 421 TIPPLMELLPLATLVSLLIETASRIEHVVNAVETLANVANFDSEEEKKRSSLDNNDHHVA 479

Query: 481 MRVFPEA 488
           MRVFPEA
Sbjct: 481 MRVFPEA 479

BLAST of Spg034543 vs. NCBI nr
Match: XP_023513501.1 (aluminum-activated malate transporter 10 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 790.4 bits (2040), Expect = 8.8e-225
Identity = 403/487 (82.75%), Postives = 433/487 (88.91%), Query Frame = 0

Query: 1   MAKAGNLEWRVNVGDGTREILRPDVPFVQKMGMRVKGFIWGLFVKAWKFLEKAWGIANSE 60
           MA  G+LEWRVNVGDG  E+LRP+  FV +M M VKGF+WGLFVK W FLEKAWGIANSE
Sbjct: 1   MASGGSLEWRVNVGDGATEVLRPEGSFVGRMVMGVKGFVWGLFVKGWMFLEKAWGIANSE 60

Query: 61  PKKAVHGLKVGLALTLVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNRV 120
           PKKAVHGLKVGLAL++VS+FYYMRPLYEG+GGNAMWAIMTVVVTFESTVGATFYKCVNRV
Sbjct: 61  PKKAVHGLKVGLALSIVSVFYYMRPLYEGIGGNAMWAIMTVVVTFESTVGATFYKCVNRV 120

Query: 121 IGTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTRFDYGTMIF 180
           IGTSLAG LGIGVHWVAAESGDRFEPI+LGISLFLLASA TFSRFIPS+KT+FDYG MIF
Sbjct: 121 IGTSLAGGLGIGVHWVAAESGDRFEPIVLGISLFLLASATTFSRFIPSIKTKFDYGAMIF 180

Query: 181 ILTFCLVSISGYRVDKLFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLHSLISRNLD 240
           ILTFCLVSIS YRVDKLFELA TRL+TIAIGTSLCI VSMLFCPMWAGSQLHSL+ RNLD
Sbjct: 181 ILTFCLVSISSYRVDKLFELAHTRLTTIAIGTSLCIIVSMLFCPMWAGSQLHSLVFRNLD 240

Query: 241 KLAHALDGCVSEYFKNDDDPEIKNEDQEGHDSKVEGYKCVLNSKASEESMANFARWEPAH 300
           KLA  LDGC+SEYFKN DDPE++NEDQE HDSK + YKCVLNSKASEESMANFARWEPAH
Sbjct: 241 KLADGLDGCLSEYFKNVDDPEMENEDQECHDSKAQSYKCVLNSKASEESMANFARWEPAH 300

Query: 301 GRFSFRHPWKKYLEIGVVMRKCAYCIEALHGCLNSEIKAPDSLKMHLAEPCKALSSSSSK 360
           GRF FRHPWKKYLE+GV MRKCAYCIEALH CLNSEIKAP+SLK+ L EPCKALS+SSSK
Sbjct: 301 GRFGFRHPWKKYLEVGVAMRKCAYCIEALHDCLNSEIKAPNSLKLLLLEPCKALSTSSSK 360

Query: 361 VLKELSVVIKKMKKSTKIDLEVSDMNIAVQELQNAIKSLPSPHMGVSSAEPEGANQDPKP 420
           VLKELS+VIK MKKSTKIDLEVSDMNIAVQELQ AI S PS     ++ E E A      
Sbjct: 361 VLKELSIVIKTMKKSTKIDLEVSDMNIAVQELQAAIASFPSTQ--TANEEHEAA------ 420

Query: 421 TNPSLMELLPLATLVSLLIETVSRIEHVVNAVEALADVANFDTEEEKKKPPSDNHNHHVA 480
           T P LMELLPLATLVSLLIET SRIEHVVNAVE LA+VANFD+EEEKK+   DN++HHVA
Sbjct: 421 TIPPLMELLPLATLVSLLIETASRIEHVVNAVETLANVANFDSEEEKKRSCLDNNDHHVA 479

Query: 481 MRVFPEA 488
           MRVFPEA
Sbjct: 481 MRVFPEA 479

BLAST of Spg034543 vs. ExPASy Swiss-Prot
Match: O23086 (Aluminum-activated malate transporter 10 OS=Arabidopsis thaliana OX=3702 GN=ALMT10 PE=3 SV=2)

HSP 1 Score: 517.3 bits (1331), Expect = 1.9e-145
Identity = 275/489 (56.24%), Postives = 356/489 (72.80%), Query Frame = 0

Query: 3   KAGNLEWRVNVGDGTREILRPDVPFVQKMGMRVKGFIWGLFV-KAWKFLEKAWGIANSEP 62
           +AG LEWR++V +GT E L P     +++ + +K  +  + + +  KF+ KAW I   +P
Sbjct: 5   EAGKLEWRISVDNGTTERLVPRSGLSKRIFLWLKDLVMKVIMERVAKFMRKAWRIGADDP 64

Query: 63  KKAVHGLKVGLALTLVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNRVI 122
            K VH LKVGLAL+LVSIFYYMRPLY+GVGGNAMWAIMTVVV FES VGATF KCVNRV+
Sbjct: 65  AKVVHCLKVGLALSLVSIFYYMRPLYDGVGGNAMWAIMTVVVVFESNVGATFCKCVNRVV 124

Query: 123 GTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTRFDYGTMIFI 182
            T LAG LGI VHWVA +SG + E  ++G S+FL A AAT+SRF+PS K RFDYG MIFI
Sbjct: 125 ATILAGSLGIAVHWVATQSG-KAEVFVIGCSVFLFAFAATYSRFVPSFKARFDYGAMIFI 184

Query: 183 LTFCLVSISGYRVDKLFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLHSLISRNLDK 242
           LTF LVS+ GYRVDKL ELA+ R+STIAIGTS+CI +++ FCP+WAGSQLH LI RNL+K
Sbjct: 185 LTFSLVSVGGYRVDKLVELAQQRVSTIAIGTSICIIITVFFCPIWAGSQLHRLIERNLEK 244

Query: 243 LAHALDGCVSEYFKNDDDPEIKNEDQEGHDSKVEGYKCVLNSKASEESM----------- 302
           LA +LDGCV+EYFK ++    +NED E  + K++G+KCVLNSK +EE+M           
Sbjct: 245 LADSLDGCVAEYFKENEVSTNRNED-ENTNMKLQGFKCVLNSKGTEEAMPLIRFSGFSFS 304

Query: 303 -ANFARWEPAHGRFSFRHPWKKYLEIGVVMRKCAYCIEALHGCLNSEIKAPDSLKMHLAE 362
            AN ARWEPAHG F+FRHPWK Y++IG  MR+CAYC+E L  C+N E +APD +K H  E
Sbjct: 305 QANLARWEPAHGSFNFRHPWKLYVKIGAAMRRCAYCLENLSICINYETEAPDQVKNHFGE 364

Query: 363 PCKALSSSSSKVLKELSVVIKKMKKSTKIDLEVSDMNIAVQELQNAIKSLP---SPHMGV 422
            C  LSS+SSK+L+EL+ ++K  +KS+K+D  V DMN AVQELQ  +K++P   +    V
Sbjct: 365 ACMKLSSASSKILRELADMMKNTRKSSKMDFLVFDMNSAVQELQETLKNVPIETNKPEEV 424

Query: 423 SSAEPEGANQDPKPTNPSLMELLPLATLVSLLIETVSRIEHVVNAVEALADVANFDTEEE 476
            S E    + + + T+ SL E+LP+ATLVSLLIE  +RI+  V AV+ LA++A+F  E++
Sbjct: 425 PSEEENKVDSEERTTSMSLHEVLPVATLVSLLIENAARIQTAVEAVDELANLADF--EQD 484

BLAST of Spg034543 vs. ExPASy Swiss-Prot
Match: Q9SJE8 (Aluminum-activated malate transporter 2 OS=Arabidopsis thaliana OX=3702 GN=ALMT2 PE=2 SV=2)

HSP 1 Score: 352.1 bits (902), Expect = 1.0e-95
Identity = 188/419 (44.87%), Postives = 269/419 (64.20%), Query Frame = 0

Query: 56  IANSEPKKAVHGLKVGLALTLVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYK 115
           +   +P++ VH  KVGLAL LVS FYY +PLY+  G NAMWA+MTVVV FE +VGAT  K
Sbjct: 14  VGKEDPRRVVHAFKVGLALALVSSFYYYQPLYDNFGVNAMWAVMTVVVVFEFSVGATLGK 73

Query: 116 CVNRVIGTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTRFDY 175
            +NR + T +AG LGIG H +A+ SG   EPI+L I +F+LA+ +TF RF P VK R+DY
Sbjct: 74  GLNRAVATLVAGGLGIGAHHLASLSGPTVEPILLAIFVFVLAALSTFVRFFPRVKARYDY 133

Query: 176 GTMIFILTFCLVSISGYRVDKLFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLHSLI 235
           G +IFILTF L+S+SG+R D++ +LA  RLST+ +G   C+ +S+  CP+WAG  LHSL+
Sbjct: 134 GVLIFILTFALISVSGFREDEILDLAHKRLSTVIMGGVSCVLISIFVCPVWAGQDLHSLL 193

Query: 236 SRNLDKLAHALDGCVSEYFKNDDDPEIKNEDQEGHDSKVEGYKCVLNSKASEESMANFAR 295
           + N D L+H L     EYF+  +D +IK  + E     +E YK VLNSK++EE++ANFA+
Sbjct: 194 ASNFDTLSHFLQEFGDEYFEATEDGDIK--EVEKRRRNLERYKSVLNSKSNEEALANFAK 253

Query: 296 WEPAHGRFSFRHPWKKYLEIGVVMRKCAYCIEALHGCLNSEIKAPDSLKMHLAEPCKALS 355
           WEP HG+F FRHPW++YL +G ++R+ AY I+AL+  +NS+++ P  +K  + EP + +S
Sbjct: 254 WEPRHGQFRFRHPWRQYLAVGALLRQSAYRIDALNSNINSDMQIPMDIKKKIEEPLRRMS 313

Query: 356 SSSSKVLKELSVVIKKMKKSTKIDLEVSDMNIAVQELQNAIKSLPSPHMGVSSAEPEGAN 415
           S S K +KE+S+ +K M  S+  D+ V +   A + L   +KS              G  
Sbjct: 314 SESGKSMKEVSISLKNMTISSSFDIHVVNSQSACKTLSTLLKS--------------GIL 373

Query: 416 QDPKPTNPSLMELLPLATLVSLLIETVSRIEHVVNAVEALADVANFDTEEEKKKPPSDN 475
            D +P     ++++ L T VSLLI+ V+  E +  +V  LA  A F   + KKKP   N
Sbjct: 374 NDVEP-----LQMISLMTTVSLLIDIVNLTEKISESVHELASAAKF---KNKKKPSKSN 408

BLAST of Spg034543 vs. ExPASy Swiss-Prot
Match: Q9SJE9 (Aluminum-activated malate transporter 1 OS=Arabidopsis thaliana OX=3702 GN=ALMT1 PE=1 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 8.0e-88
Identity = 185/441 (41.95%), Postives = 269/441 (61.00%), Query Frame = 0

Query: 56  IANSEPKKAVHGLKVGLALTLVSIFYYMR---PLYEGVGGNAMWAIMTVVVTFESTVGAT 115
           + N +P++ +H  KVGLAL LVS FYY +   P  +  G NAMWA+MTVVV FE +VGAT
Sbjct: 14  VGNEDPRRIIHAFKVGLALVLVSSFYYYQPFGPFTDYFGINAMWAVMTVVVVFEFSVGAT 73

Query: 116 FYKCVNRVIGTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTR 175
             K +NR + T +AG LGIG H +A  SG   EPI+L + +F+ A+ +TF RF P VKT+
Sbjct: 74  LGKGLNRGVATLVAGGLGIGAHQLARLSGATVEPILLVMLVFVQAALSTFVRFFPWVKTK 133

Query: 176 FDYGTMIFILTFCLVSISGYRVDKLFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLH 235
           FDYG +IFILTF L+S+SG+R +++ +LA +RLST+ IG   CI +S+  CP+WAG  LH
Sbjct: 134 FDYGILIFILTFALISLSGFRDEEIMDLAESRLSTVVIGGVSCILISIFVCPVWAGQDLH 193

Query: 236 SLISRNLDKLAHALDGCVSEYFKNDDDPEIKNEDQEGHDSKVEGYKCVLNSKASEESMAN 295
           SL++ N D L+H L     EYF+  +  + K    E     +E YK VL+SK+ EE++AN
Sbjct: 194 SLLASNFDTLSHFLQDFGDEYFEAREKGDYK--VVEKRKKNLERYKSVLDSKSDEEALAN 253

Query: 296 FARWEPAHGRFSFRHPWKKYLEIGVVMRKCAYCIEALHGCLNSEIKAPDSLKMHLAEPCK 355
           +A WEP HG+F FRHPWK+Y+ +G ++R+CAY I+AL+  +NS+ + P  +K  L  P +
Sbjct: 254 YAEWEPPHGQFRFRHPWKQYVAVGALLRQCAYRIDALNSYINSDFQIPVDIKKKLETPLR 313

Query: 356 ALSSSSSKVLKELSVVIKKMKKSTKIDLEVSDMNIAVQELQNAIKSLPSPHMGVSSAEPE 415
            +SS S   +KE+S+ +K+M KS+  D+ VS+   A + L   +KS              
Sbjct: 314 RMSSESGNSMKEMSISLKQMIKSSSSDIHVSNSQAACKSLSTLLKS-------------- 373

Query: 416 GANQDPKPTNPSLMELLPLATLVSLLIETVSRIEHVVNAVEALADVANF----------- 475
           G   D +P     ++++ L T VS+LI+ V+  E +  +V  LA  A F           
Sbjct: 374 GILNDVEP-----LQMISLMTTVSMLIDIVNLTEKISESVHELASAARFKNKMRPTVLYE 433

Query: 476 --DTEEEKKKPPSDNH-NHHV 480
             D+    +  P D+H +HHV
Sbjct: 434 KSDSGSIGRAMPIDSHEDHHV 433

BLAST of Spg034543 vs. ExPASy Swiss-Prot
Match: Q9SRM9 (Aluminum-activated malate transporter 8 OS=Arabidopsis thaliana OX=3702 GN=ALMT8 PE=3 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 1.4e-87
Identity = 181/412 (43.93%), Postives = 258/412 (62.62%), Query Frame = 0

Query: 57  ANSEPKKAVHGLKVGLALTLVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKC 116
           A  +P++ +H +KVG+ALTLVS+ YY+RPLY   G   MWAI+TVVV FE TVG T  K 
Sbjct: 38  AKDDPRRIIHSMKVGVALTLVSLLYYVRPLYISFGVTGMWAILTVVVVFEFTVGGTLSKG 97

Query: 117 VNRVIGTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTRFDYG 176
           +NR   T +AG LG+G   +A   G + EPI+LGI +F L +AATFSRF P +K R+DYG
Sbjct: 98  LNRGFATLIAGALGVGAVHLARFFGHQGEPIVLGILVFSLGAAATFSRFFPRIKQRYDYG 157

Query: 177 TMIFILTFCLVSISGYRVDKLFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLHSLIS 236
            +IFILTF  V+ISGYR D++  +A  RLSTI IG ++CI VS+  CP+WAG  LH +I+
Sbjct: 158 ALIFILTFSFVAISGYRTDEILIMAYQRLSTILIGGTICILVSIFICPVWAGEDLHKMIA 217

Query: 237 RNLDKLAHALDGCVSEYFKNDDDPEIKNEDQEGHDSKVEGYKCVLNSKASEESMANFARW 296
            N++KLA  L+G   EYF+    PE  +++     S V  YK +L SK++E+S+AN ARW
Sbjct: 218 NNINKLAKYLEGFEGEYFQ----PEKISKET---SSCVREYKSILTSKSTEDSLANLARW 277

Query: 297 EPAHGRFSFRHPWKKYLEIGVVMRKCAYCIEALHGCLNSEIKAPDSLKMHLAEPCKALSS 356
           EP HGRF  RHPWKKYL+I  ++R+CA  +E L+G + S  KAP   +  + EP   +S 
Sbjct: 278 EPGHGRFRLRHPWKKYLKIAGLVRQCAVHLEILNGYVLSNDKAPQEFESKIQEPITTMSR 337

Query: 357 SSSKVLKELSVVIKKMKKSTKIDLEVSDMNIAVQELQNAIKSLPSPHMGVSSAEPEGANQ 416
              + LK ++  IK M+  +      + +N  +   + AIK+L    + + S+ PE    
Sbjct: 338 EVGEALKAIAKSIKTMRNDS------ACVNAHIDNSKKAIKNL---KIALKSSYPE---- 397

Query: 417 DPKPTNPSLMELLPLATLVSLLIETVSRIEHVVNAVEALADVANFDTEEEKK 469
               T   L+E++P  T+ S+LIE V+ +E +  AVE  + +A+F    + K
Sbjct: 398 ----TYKDLLEIIPGVTMASILIEVVNCVEKIYEAVEEFSGLAHFKETLDSK 425

BLAST of Spg034543 vs. ExPASy Swiss-Prot
Match: Q9XIN1 (Aluminum-activated malate transporter 7 OS=Arabidopsis thaliana OX=3702 GN=ALMT7 PE=3 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 1.5e-86
Identity = 180/437 (41.19%), Postives = 259/437 (59.27%), Query Frame = 0

Query: 56  IANSEPKKAVHGLKVGLALTLVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYK 115
           +A  +P++ VH  KVGL L LVS FYY +PLY+  G NAMWA+MTVVV FE +VGAT  K
Sbjct: 14  VAKEDPRRVVHSFKVGLVLALVSSFYYYQPLYDSFGVNAMWAVMTVVVVFEFSVGATLGK 73

Query: 116 CVNRVIGTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTRFDY 175
            +NRV  T  AG LGIG H +A+ SG   EPI+L + +F+ A+ +TF RF P VK R+DY
Sbjct: 74  GLNRVAATLFAGGLGIGAHHLASMSGPTGEPILLAVFVFVQAALSTFVRFFPRVKARYDY 133

Query: 176 GTMIFILTFCLVSISGYRVDKLFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLHSLI 235
             +IFILTF L+S+SG+R +++ +L   R+ST+ IG   C+ +S+  CP+WAG  LHSLI
Sbjct: 134 SLLIFILTFALISVSGFREEQVVKLTHKRISTVIIGGLSCVIISIFVCPVWAGQDLHSLI 193

Query: 236 SRNLDKLAHALDGCVSEYFKND--------------------------DDPEIKNEDQEG 295
           + N +KL+  L G    Y  +D                          ++   K  D+  
Sbjct: 194 ASNFEKLSFFLLGNSFHYVSSDLNSITLLRKIKSWRLADFGDKYCEVVENDGAKEVDKRK 253

Query: 296 HDSKVEGYKCVLNSKASEESMANFARWEPAHGRFSFRHPWKKYLEIGVVMRKCAYCIEAL 355
            D   + YK VLNSK++EES+ANFA+WEP HG+F FRHPWK+YL +G ++R+CAY I AL
Sbjct: 254 KD--FDNYKSVLNSKSNEESLANFAKWEPGHGQFRFRHPWKQYLAVGELIRQCAYRIHAL 313

Query: 356 HGCLNSEIKAPDSLKMHLAEPCKALSSSSSKVLKELSVVIKKMKKSTKIDLEVSDMNIAV 415
           +  LN++ +    +K  L EP + +S  S K +KE+S+ +KKM K +  DL V +   A 
Sbjct: 314 NSYLNADNQVSVDIKKKLGEPLRRMSLESGKAMKEMSISLKKMTKPSSSDLHVQNAKSAC 373

Query: 416 QELQNAIKSLPSPHMGVSSAEPEGANQDPKPTNPSLMELLPLATLVSLLIETVSRIEHVV 467
           + L N + S              G  ++ +P     +EL+ L T +SLLI+ ++  E ++
Sbjct: 374 KSLTNLLNS--------------GILKEVEP-----LELVSLLTAISLLIDIINLTEKIL 429

BLAST of Spg034543 vs. ExPASy TrEMBL
Match: A0A0A0KCJ2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G106810 PE=3 SV=1)

HSP 1 Score: 796.2 bits (2055), Expect = 7.7e-227
Identity = 409/496 (82.46%), Postives = 447/496 (90.12%), Query Frame = 0

Query: 1   MAKAGNLEWRVNVGDGTREILRPDVPFVQKMGMRVKGFIW-GLFVKAWKFLEKAWGIANS 60
           M   GNLEWRVN+GDGT EILRP+  F++ + MR+K FIW GL +KAWKFLEKAWGIANS
Sbjct: 1   MTSNGNLEWRVNMGDGTTEILRPEASFMKNIMMRIKNFIWGGLLLKAWKFLEKAWGIANS 60

Query: 61  EPKKAVHGLKVGLALTLVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNR 120
           EPKKAVHGLKVGLALT+VSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNR
Sbjct: 61  EPKKAVHGLKVGLALTIVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNR 120

Query: 121 VIGTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTRFDYGTMI 180
           VIGTSLAGCLGIGVHW+AAESGD+FEPIILGISLFLLAS  TFSRFIPSVK+RFDYG MI
Sbjct: 121 VIGTSLAGCLGIGVHWIAAESGDKFEPIILGISLFLLASVTTFSRFIPSVKSRFDYGAMI 180

Query: 181 FILTFCLVSISGYRVDKLFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLHSLISRNL 240
           F+LTFCLVSISGYRV+KLFELARTR+STIAIGTSLCIFVSMLFCP+WAGSQL SL +RNL
Sbjct: 181 FVLTFCLVSISGYRVEKLFELARTRISTIAIGTSLCIFVSMLFCPIWAGSQLQSLTARNL 240

Query: 241 DKLAHALDGCVSEYFKNDDDPE--IKNEDQEGHDSKVEGYKCVLNSKASEESMANFARWE 300
           DKLAH+LDGCVSEYF+NDDD E    NED++ ++SKVEGYKCVLNSKASEESMANFARWE
Sbjct: 241 DKLAHSLDGCVSEYFENDDDTEEMKNNEDEKNNNSKVEGYKCVLNSKASEESMANFARWE 300

Query: 301 PAHGRFSFRHPWKKYLEIGVVMRKCAYCIEALHGCLNSEIKAPDSLKMHLAEPCKALSSS 360
           PAHGRF FRHPWKKYLE+G VMRK AYCIEALHGCLNSEI+AP+SLK+HLAEPCKALSSS
Sbjct: 301 PAHGRFGFRHPWKKYLEVGGVMRKSAYCIEALHGCLNSEIQAPNSLKLHLAEPCKALSSS 360

Query: 361 SSKVLKELSVVIKKMKKSTKIDLEVSDMNIAVQELQNAIKSLPSPHMGV--SSAEPEGAN 420
           SS+VLKELS+VIKKMKKSTKID  VS+MN+AVQELQNAIKS PS  M V  S  E E  N
Sbjct: 361 SSEVLKELSIVIKKMKKSTKIDFLVSNMNVAVQELQNAIKSFPSTQMEVSLSEQEEEANN 420

Query: 421 QDPK---PTNPSLMELLPLATLVSLLIETVSRIEHVVNAVEALADVANFDTEEEKKKP-P 480
           +D K    T P LM+LLPLATLVSLLIET SRIEHVVNAVE LA+VAN+D+E+EKKKP  
Sbjct: 421 EDHKAATTTIPPLMKLLPLATLVSLLIETTSRIEHVVNAVETLANVANYDSEDEKKKPSS 480

Query: 481 SDNHNHHVAMRVFPEA 488
           SDNH+H+VAMRVFPEA
Sbjct: 481 SDNHDHNVAMRVFPEA 496

BLAST of Spg034543 vs. ExPASy TrEMBL
Match: A0A1S3CB02 (aluminum-activated malate transporter 10 OS=Cucumis melo OX=3656 GN=LOC103498794 PE=3 SV=1)

HSP 1 Score: 793.1 bits (2047), Expect = 6.6e-226
Identity = 411/498 (82.53%), Postives = 448/498 (89.96%), Query Frame = 0

Query: 1   MAKAGNLEWRVNVGDGTREILRPDVPFVQKMGMRVKGFIW-GLFVKAWKFLEKAWGIANS 60
           MA  GNLEWRVN+GDGT EILRP   F++ + MR+K FIW GL +KAWKFLEKAWGIANS
Sbjct: 1   MASNGNLEWRVNMGDGTTEILRPQASFMKNIMMRIKDFIWGGLLLKAWKFLEKAWGIANS 60

Query: 61  EPKKAVHGLKVGLALTLVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNR 120
           EPKK VHGLKVGLALT+VSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNR
Sbjct: 61  EPKKVVHGLKVGLALTIVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNR 120

Query: 121 VIGTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTRFDYGTMI 180
           VIGTSLAGCLGIGVHW+AAESGD+FEPIILGISLFLLASAATFSRFIPSVK+RFDYG MI
Sbjct: 121 VIGTSLAGCLGIGVHWIAAESGDKFEPIILGISLFLLASAATFSRFIPSVKSRFDYGAMI 180

Query: 181 FILTFCLVSISGYRVDKLFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLHSLISRNL 240
           F+LTFCLVSISGYRV+KL ELARTR+STIAIGTSLCIFVSMLFCP+WAGSQL SL +RNL
Sbjct: 181 FVLTFCLVSISGYRVEKLLELARTRISTIAIGTSLCIFVSMLFCPIWAGSQLQSLTARNL 240

Query: 241 DKLAHALDGCVSEYFKNDDDP-EIKNEDQEGHDSKVEGYKCVLNSKASEESMANFARWEP 300
           DKLA +LDGCV EYF+NDDD  E+KNEDQ+ ++SKVEGYKCVLNSKASEESMANFARWEP
Sbjct: 241 DKLALSLDGCVCEYFENDDDTGEMKNEDQKNNNSKVEGYKCVLNSKASEESMANFARWEP 300

Query: 301 AHGRFSFRHPWKKYLEIGVVMRKCAYCIEALHGCLNSEIKAPDSLKMHLAEPCKALSSSS 360
           AHGRF FRHPWKKYLE+G VMRK AYCIEALHGCLNSEI+AP+SLK+HLAEPCKALSSSS
Sbjct: 301 AHGRFGFRHPWKKYLEVGGVMRKSAYCIEALHGCLNSEIQAPNSLKLHLAEPCKALSSSS 360

Query: 361 SKVLKELSVVIKKMKKSTKIDLEVSDMNIAVQELQNAIKSLPSPHMGVSSAE---PEGAN 420
           S+VLKELS+VIKKMKKSTKID  VS+MNIAVQELQNAIKSLPS  M VS +E    E  N
Sbjct: 361 SQVLKELSIVIKKMKKSTKIDFLVSNMNIAVQELQNAIKSLPSTQMEVSLSEQDHQEANN 420

Query: 421 QD-----PKPTNPSLMELLPLATLVSLLIETVSRIEHVVNAVEALADVANFDTEEEKKKP 480
           +D        T P LMELLPLATLVSLLIET SRIEHVVNAVE LA+VA++D+E+EKKKP
Sbjct: 421 EDHHKATTAATIPPLMELLPLATLVSLLIETTSRIEHVVNAVETLANVADYDSEDEKKKP 480

Query: 481 -PSDNHNHHVAMRVFPEA 488
             SDNH+H+VAMRVFPEA
Sbjct: 481 SSSDNHDHNVAMRVFPEA 498

BLAST of Spg034543 vs. ExPASy TrEMBL
Match: A0A6J1H8F3 (aluminum-activated malate transporter 10 OS=Cucurbita moschata OX=3662 GN=LOC111461009 PE=3 SV=1)

HSP 1 Score: 791.2 bits (2042), Expect = 2.5e-225
Identity = 404/487 (82.96%), Postives = 433/487 (88.91%), Query Frame = 0

Query: 1   MAKAGNLEWRVNVGDGTREILRPDVPFVQKMGMRVKGFIWGLFVKAWKFLEKAWGIANSE 60
           MA  G+LEWRVNVGDG  E+LRP+  FV +M M VKGF+WGLFVK W FLEKAWGIANSE
Sbjct: 1   MASGGSLEWRVNVGDGATEVLRPEGSFVGRMVMGVKGFVWGLFVKGWMFLEKAWGIANSE 60

Query: 61  PKKAVHGLKVGLALTLVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNRV 120
           PKKAVHGLKVGLAL++VS+FYYMRPLYEG+GGNAMWAIMTVVVTFESTVGATFYKCVNRV
Sbjct: 61  PKKAVHGLKVGLALSIVSVFYYMRPLYEGIGGNAMWAIMTVVVTFESTVGATFYKCVNRV 120

Query: 121 IGTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTRFDYGTMIF 180
           IGTSLAG LGIGVHWVAAESGDRFEPIILGISLFLLASA TFSRFIPS+KT+FDYG MIF
Sbjct: 121 IGTSLAGGLGIGVHWVAAESGDRFEPIILGISLFLLASATTFSRFIPSIKTKFDYGAMIF 180

Query: 181 ILTFCLVSISGYRVDKLFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLHSLISRNLD 240
           ILTFCLVSIS YRVDKLFELA TRL+TIAIGTSLCI VSMLFCPMWAGSQLHSL+ RNLD
Sbjct: 181 ILTFCLVSISSYRVDKLFELAHTRLTTIAIGTSLCIIVSMLFCPMWAGSQLHSLVFRNLD 240

Query: 241 KLAHALDGCVSEYFKNDDDPEIKNEDQEGHDSKVEGYKCVLNSKASEESMANFARWEPAH 300
           KLA  LDGCVSEYFKN DDPE++NE+QE HDSK + YKCVLNSKASEESMANFARWEPAH
Sbjct: 241 KLADGLDGCVSEYFKNVDDPEMENENQECHDSKAQSYKCVLNSKASEESMANFARWEPAH 300

Query: 301 GRFSFRHPWKKYLEIGVVMRKCAYCIEALHGCLNSEIKAPDSLKMHLAEPCKALSSSSSK 360
           GRF FRHPWKKYLE+GV MRKCAYCIEALH CLNSEIKAP+SLK+ L EPCKALS+SSSK
Sbjct: 301 GRFGFRHPWKKYLEVGVAMRKCAYCIEALHDCLNSEIKAPNSLKLLLVEPCKALSTSSSK 360

Query: 361 VLKELSVVIKKMKKSTKIDLEVSDMNIAVQELQNAIKSLPSPHMGVSSAEPEGANQDPKP 420
           VLKELS+VIK MKKSTKIDLEVSDMNIAVQELQ AI S PS     ++ E E A      
Sbjct: 361 VLKELSIVIKTMKKSTKIDLEVSDMNIAVQELQAAIASFPSTQ--TANEEHEAA------ 420

Query: 421 TNPSLMELLPLATLVSLLIETVSRIEHVVNAVEALADVANFDTEEEKKKPPSDNHNHHVA 480
           T P LMELLPLATLVSLLIET SRIEHVVNAVE LA+VANFD+EEEKK+   DN++HHVA
Sbjct: 421 TIPPLMELLPLATLVSLLIETASRIEHVVNAVETLANVANFDSEEEKKRSSLDNNDHHVA 479

Query: 481 MRVFPEA 488
           MRVFPEA
Sbjct: 481 MRVFPEA 479

BLAST of Spg034543 vs. ExPASy TrEMBL
Match: A0A6J1KS06 (aluminum-activated malate transporter 10 OS=Cucurbita maxima OX=3661 GN=LOC111498132 PE=3 SV=1)

HSP 1 Score: 782.3 bits (2019), Expect = 1.2e-222
Identity = 401/487 (82.34%), Postives = 431/487 (88.50%), Query Frame = 0

Query: 1   MAKAGNLEWRVNVGDGTREILRPDVPFVQKMGMRVKGFIWGLFVKAWKFLEKAWGIANSE 60
           MA  G+LEWRVNVGDG  E+LRP+  FV +M M VKGF+WGLF+K W FLEKAWGIANSE
Sbjct: 1   MASGGSLEWRVNVGDGATEVLRPEGSFVSRMVMGVKGFVWGLFMKGWMFLEKAWGIANSE 60

Query: 61  PKKAVHGLKVGLALTLVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNRV 120
           PKKAVHGLKVGLAL++VS+FYYMRPLYEG+GGNAMWAIMTVVVTFES+VGATFYKCVNRV
Sbjct: 61  PKKAVHGLKVGLALSIVSVFYYMRPLYEGIGGNAMWAIMTVVVTFESSVGATFYKCVNRV 120

Query: 121 IGTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTRFDYGTMIF 180
           IGTSLAG LGIGVHWVAAESGDRFEPIILGISLFLLASA TFSRFIPS+KT+FDYG MIF
Sbjct: 121 IGTSLAGGLGIGVHWVAAESGDRFEPIILGISLFLLASATTFSRFIPSIKTKFDYGAMIF 180

Query: 181 ILTFCLVSISGYRVDKLFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLHSLISRNLD 240
           ILTFCLVSIS YRVDKLFELA TRL+TIAIGTSLCI +SMLFCPMWAGSQLHSL+ RNLD
Sbjct: 181 ILTFCLVSISSYRVDKLFELAHTRLTTIAIGTSLCIIISMLFCPMWAGSQLHSLVFRNLD 240

Query: 241 KLAHALDGCVSEYFKNDDDPEIKNEDQEGHDSKVEGYKCVLNSKASEESMANFARWEPAH 300
           KLA  LDGCVSEYFKN DDPE++NEDQE HDSK + YKCVLNSKASEESMANFARWEPAH
Sbjct: 241 KLADGLDGCVSEYFKNVDDPEMENEDQECHDSKAQSYKCVLNSKASEESMANFARWEPAH 300

Query: 301 GRFSFRHPWKKYLEIGVVMRKCAYCIEALHGCLNSEIKAPDSLKMHLAEPCKALSSSSSK 360
           GRF FRHPWK+YLE+GV MRKCAYCIEALH CLNSEIKAP+SLK+ L EPCKALS+SSSK
Sbjct: 301 GRFGFRHPWKQYLEVGVAMRKCAYCIEALHDCLNSEIKAPNSLKLLLVEPCKALSTSSSK 360

Query: 361 VLKELSVVIKKMKKSTKIDLEVSDMNIAVQELQNAIKSLPSPHMGVSSAEPEGANQDPKP 420
           VLKELS+VIK MKKSTKIDLEVSDMNIAVQELQ AI S PS     ++ E E A      
Sbjct: 361 VLKELSIVIKTMKKSTKIDLEVSDMNIAVQELQAAIASFPSTQ--TANEEHEAA------ 420

Query: 421 TNPSLMELLPLATLVSLLIETVSRIEHVVNAVEALADVANFDTEEEKKKPPSDNHNHHVA 480
           T P LMELLPLATLVSLLIET SRIEHVVNAVE LA+VANFD+EEEKK+   DN N HVA
Sbjct: 421 TIPPLMELLPLATLVSLLIETASRIEHVVNAVETLANVANFDSEEEKKRSCLDN-NDHVA 478

Query: 481 MRVFPEA 488
           MRVFPEA
Sbjct: 481 MRVFPEA 478

BLAST of Spg034543 vs. ExPASy TrEMBL
Match: A0A6J1DX41 (aluminum-activated malate transporter 10 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111025265 PE=3 SV=1)

HSP 1 Score: 719.5 bits (1856), Expect = 9.2e-204
Identity = 377/491 (76.78%), Postives = 416/491 (84.73%), Query Frame = 0

Query: 1   MAKAGNLEWRVNVGDGTREILRPDVPFVQKMGMRVKGFIWGLFVKAWKFLEKAWGIANSE 60
           MA AGNLEWRVN+GDGT EIL  +  F  K+ M +K  I  LFVK  KFLEKA GIA SE
Sbjct: 1   MASAGNLEWRVNLGDGTTEILEAEASFGHKLVMGIKSLIMELFVKCRKFLEKAGGIAKSE 60

Query: 61  PKKAVHGLKVGLALTLVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNRV 120
           PKK VHGLKVGLALTLVSIFYYMRPLYEG+GG+AMWAIMTVVVTFE +VGA+ YKCVNRV
Sbjct: 61  PKKGVHGLKVGLALTLVSIFYYMRPLYEGLGGSAMWAIMTVVVTFEYSVGASLYKCVNRV 120

Query: 121 IGTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTRFDYGTMIF 180
           +GTSLAG LGIGVHWVAAE GDRFEPIILGISLFLLASAATFSRFIPSVK+RFDYG MIF
Sbjct: 121 VGTSLAGGLGIGVHWVAAECGDRFEPIILGISLFLLASAATFSRFIPSVKSRFDYGAMIF 180

Query: 181 ILTFCLVSISGYRVDKLFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLHSLISRNLD 240
           +LTFCLVSIS YRVD+L ELARTR+ TIA+GT++C+ VSML CP+WAG QLHSL++RN+D
Sbjct: 181 VLTFCLVSISSYRVDELLELARTRILTIAVGTAICLVVSMLVCPVWAGGQLHSLLARNID 240

Query: 241 KLAHALDGCVSEYFKNDDDPEIKNEDQEGHDSKVEGYKCVLNSKASEESMANFARWEPAH 300
           KLA A+ GC+SEYFK+ D PE+KNED+E H SK++ YKCVLNSKASEESMANFARWEPAH
Sbjct: 241 KLADAIHGCISEYFKDKDCPEMKNEDREDHASKIQAYKCVLNSKASEESMANFARWEPAH 300

Query: 301 GRFSFRHPWKKYLEIGVVMRKCAYCIEALHGCLNSEIKAPDSLKMHLAEPCKALSSSSSK 360
           GRF FRHPWK YLE+GV MR+CAYCIEAL+G L+SEIKAP+SLK+HLA PCKALSS  SK
Sbjct: 301 GRFGFRHPWKFYLEVGVAMRRCAYCIEALNGRLDSEIKAPESLKLHLATPCKALSSCCSK 360

Query: 361 VLKELSVVIKKMKKSTKIDLEVSDMNIAVQELQNAIKSLPSPHMGVSSAEPEGANQDPKP 420
           V KELS VIK MKKS KID EVSDMN AVQELQN IKSLP   M VS AEP GAN +   
Sbjct: 361 VFKELSTVIKTMKKSNKIDFEVSDMNFAVQELQNTIKSLP---MAVSLAEPNGANGE--A 420

Query: 421 TNPSLMELLPLATLVSLLIETVSRIEHVVNAVEALADVANFD-TEEEKKK--PPSDNH-N 480
           T P LMELL LATLVSLLIET SRIEHVVNAVE LADVA FD  ++EKKK  P SDN  +
Sbjct: 421 TIPPLMELLSLATLVSLLIETASRIEHVVNAVEKLADVAKFDFADDEKKKSNPSSDNRDH 480

Query: 481 HHVAMRVFPEA 488
           HH AM+VFPEA
Sbjct: 481 HHAAMKVFPEA 486

BLAST of Spg034543 vs. TAIR 10
Match: AT4G00910.1 (Aluminium activated malate transporter family protein )

HSP 1 Score: 517.3 bits (1331), Expect = 1.3e-146
Identity = 275/489 (56.24%), Postives = 356/489 (72.80%), Query Frame = 0

Query: 3   KAGNLEWRVNVGDGTREILRPDVPFVQKMGMRVKGFIWGLFV-KAWKFLEKAWGIANSEP 62
           +AG LEWR++V +GT E L P     +++ + +K  +  + + +  KF+ KAW I   +P
Sbjct: 5   EAGKLEWRISVDNGTTERLVPRSGLSKRIFLWLKDLVMKVIMERVAKFMRKAWRIGADDP 64

Query: 63  KKAVHGLKVGLALTLVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKCVNRVI 122
            K VH LKVGLAL+LVSIFYYMRPLY+GVGGNAMWAIMTVVV FES VGATF KCVNRV+
Sbjct: 65  AKVVHCLKVGLALSLVSIFYYMRPLYDGVGGNAMWAIMTVVVVFESNVGATFCKCVNRVV 124

Query: 123 GTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTRFDYGTMIFI 182
            T LAG LGI VHWVA +SG + E  ++G S+FL A AAT+SRF+PS K RFDYG MIFI
Sbjct: 125 ATILAGSLGIAVHWVATQSG-KAEVFVIGCSVFLFAFAATYSRFVPSFKARFDYGAMIFI 184

Query: 183 LTFCLVSISGYRVDKLFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLHSLISRNLDK 242
           LTF LVS+ GYRVDKL ELA+ R+STIAIGTS+CI +++ FCP+WAGSQLH LI RNL+K
Sbjct: 185 LTFSLVSVGGYRVDKLVELAQQRVSTIAIGTSICIIITVFFCPIWAGSQLHRLIERNLEK 244

Query: 243 LAHALDGCVSEYFKNDDDPEIKNEDQEGHDSKVEGYKCVLNSKASEESM----------- 302
           LA +LDGCV+EYFK ++    +NED E  + K++G+KCVLNSK +EE+M           
Sbjct: 245 LADSLDGCVAEYFKENEVSTNRNED-ENTNMKLQGFKCVLNSKGTEEAMPLIRFSGFSFS 304

Query: 303 -ANFARWEPAHGRFSFRHPWKKYLEIGVVMRKCAYCIEALHGCLNSEIKAPDSLKMHLAE 362
            AN ARWEPAHG F+FRHPWK Y++IG  MR+CAYC+E L  C+N E +APD +K H  E
Sbjct: 305 QANLARWEPAHGSFNFRHPWKLYVKIGAAMRRCAYCLENLSICINYETEAPDQVKNHFGE 364

Query: 363 PCKALSSSSSKVLKELSVVIKKMKKSTKIDLEVSDMNIAVQELQNAIKSLP---SPHMGV 422
            C  LSS+SSK+L+EL+ ++K  +KS+K+D  V DMN AVQELQ  +K++P   +    V
Sbjct: 365 ACMKLSSASSKILRELADMMKNTRKSSKMDFLVFDMNSAVQELQETLKNVPIETNKPEEV 424

Query: 423 SSAEPEGANQDPKPTNPSLMELLPLATLVSLLIETVSRIEHVVNAVEALADVANFDTEEE 476
            S E    + + + T+ SL E+LP+ATLVSLLIE  +RI+  V AV+ LA++A+F  E++
Sbjct: 425 PSEEENKVDSEERTTSMSLHEVLPVATLVSLLIENAARIQTAVEAVDELANLADF--EQD 484

BLAST of Spg034543 vs. TAIR 10
Match: AT1G08440.1 (Aluminium activated malate transporter family protein )

HSP 1 Score: 352.1 bits (902), Expect = 7.4e-97
Identity = 188/419 (44.87%), Postives = 269/419 (64.20%), Query Frame = 0

Query: 56  IANSEPKKAVHGLKVGLALTLVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYK 115
           +   +P++ VH  KVGLAL LVS FYY +PLY+  G NAMWA+MTVVV FE +VGAT  K
Sbjct: 14  VGKEDPRRVVHAFKVGLALALVSSFYYYQPLYDNFGVNAMWAVMTVVVVFEFSVGATLGK 73

Query: 116 CVNRVIGTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTRFDY 175
            +NR + T +AG LGIG H +A+ SG   EPI+L I +F+LA+ +TF RF P VK R+DY
Sbjct: 74  GLNRAVATLVAGGLGIGAHHLASLSGPTVEPILLAIFVFVLAALSTFVRFFPRVKARYDY 133

Query: 176 GTMIFILTFCLVSISGYRVDKLFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLHSLI 235
           G +IFILTF L+S+SG+R D++ +LA  RLST+ +G   C+ +S+  CP+WAG  LHSL+
Sbjct: 134 GVLIFILTFALISVSGFREDEILDLAHKRLSTVIMGGVSCVLISIFVCPVWAGQDLHSLL 193

Query: 236 SRNLDKLAHALDGCVSEYFKNDDDPEIKNEDQEGHDSKVEGYKCVLNSKASEESMANFAR 295
           + N D L+H L     EYF+  +D +IK  + E     +E YK VLNSK++EE++ANFA+
Sbjct: 194 ASNFDTLSHFLQEFGDEYFEATEDGDIK--EVEKRRRNLERYKSVLNSKSNEEALANFAK 253

Query: 296 WEPAHGRFSFRHPWKKYLEIGVVMRKCAYCIEALHGCLNSEIKAPDSLKMHLAEPCKALS 355
           WEP HG+F FRHPW++YL +G ++R+ AY I+AL+  +NS+++ P  +K  + EP + +S
Sbjct: 254 WEPRHGQFRFRHPWRQYLAVGALLRQSAYRIDALNSNINSDMQIPMDIKKKIEEPLRRMS 313

Query: 356 SSSSKVLKELSVVIKKMKKSTKIDLEVSDMNIAVQELQNAIKSLPSPHMGVSSAEPEGAN 415
           S S K +KE+S+ +K M  S+  D+ V +   A + L   +KS              G  
Sbjct: 314 SESGKSMKEVSISLKNMTISSSFDIHVVNSQSACKTLSTLLKS--------------GIL 373

Query: 416 QDPKPTNPSLMELLPLATLVSLLIETVSRIEHVVNAVEALADVANFDTEEEKKKPPSDN 475
            D +P     ++++ L T VSLLI+ V+  E +  +V  LA  A F   + KKKP   N
Sbjct: 374 NDVEP-----LQMISLMTTVSLLIDIVNLTEKISESVHELASAAKF---KNKKKPSKSN 408

BLAST of Spg034543 vs. TAIR 10
Match: AT1G08430.1 (aluminum-activated malate transporter 1 )

HSP 1 Score: 325.9 bits (834), Expect = 5.7e-89
Identity = 185/441 (41.95%), Postives = 269/441 (61.00%), Query Frame = 0

Query: 56  IANSEPKKAVHGLKVGLALTLVSIFYYMR---PLYEGVGGNAMWAIMTVVVTFESTVGAT 115
           + N +P++ +H  KVGLAL LVS FYY +   P  +  G NAMWA+MTVVV FE +VGAT
Sbjct: 14  VGNEDPRRIIHAFKVGLALVLVSSFYYYQPFGPFTDYFGINAMWAVMTVVVVFEFSVGAT 73

Query: 116 FYKCVNRVIGTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTR 175
             K +NR + T +AG LGIG H +A  SG   EPI+L + +F+ A+ +TF RF P VKT+
Sbjct: 74  LGKGLNRGVATLVAGGLGIGAHQLARLSGATVEPILLVMLVFVQAALSTFVRFFPWVKTK 133

Query: 176 FDYGTMIFILTFCLVSISGYRVDKLFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLH 235
           FDYG +IFILTF L+S+SG+R +++ +LA +RLST+ IG   CI +S+  CP+WAG  LH
Sbjct: 134 FDYGILIFILTFALISLSGFRDEEIMDLAESRLSTVVIGGVSCILISIFVCPVWAGQDLH 193

Query: 236 SLISRNLDKLAHALDGCVSEYFKNDDDPEIKNEDQEGHDSKVEGYKCVLNSKASEESMAN 295
           SL++ N D L+H L     EYF+  +  + K    E     +E YK VL+SK+ EE++AN
Sbjct: 194 SLLASNFDTLSHFLQDFGDEYFEAREKGDYK--VVEKRKKNLERYKSVLDSKSDEEALAN 253

Query: 296 FARWEPAHGRFSFRHPWKKYLEIGVVMRKCAYCIEALHGCLNSEIKAPDSLKMHLAEPCK 355
           +A WEP HG+F FRHPWK+Y+ +G ++R+CAY I+AL+  +NS+ + P  +K  L  P +
Sbjct: 254 YAEWEPPHGQFRFRHPWKQYVAVGALLRQCAYRIDALNSYINSDFQIPVDIKKKLETPLR 313

Query: 356 ALSSSSSKVLKELSVVIKKMKKSTKIDLEVSDMNIAVQELQNAIKSLPSPHMGVSSAEPE 415
            +SS S   +KE+S+ +K+M KS+  D+ VS+   A + L   +KS              
Sbjct: 314 RMSSESGNSMKEMSISLKQMIKSSSSDIHVSNSQAACKSLSTLLKS-------------- 373

Query: 416 GANQDPKPTNPSLMELLPLATLVSLLIETVSRIEHVVNAVEALADVANF----------- 475
           G   D +P     ++++ L T VS+LI+ V+  E +  +V  LA  A F           
Sbjct: 374 GILNDVEP-----LQMISLMTTVSMLIDIVNLTEKISESVHELASAARFKNKMRPTVLYE 433

Query: 476 --DTEEEKKKPPSDNH-NHHV 480
             D+    +  P D+H +HHV
Sbjct: 434 KSDSGSIGRAMPIDSHEDHHV 433

BLAST of Spg034543 vs. TAIR 10
Match: AT3G11680.1 (Aluminium activated malate transporter family protein )

HSP 1 Score: 325.1 bits (832), Expect = 9.7e-89
Identity = 181/412 (43.93%), Postives = 258/412 (62.62%), Query Frame = 0

Query: 57  ANSEPKKAVHGLKVGLALTLVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYKC 116
           A  +P++ +H +KVG+ALTLVS+ YY+RPLY   G   MWAI+TVVV FE TVG T  K 
Sbjct: 38  AKDDPRRIIHSMKVGVALTLVSLLYYVRPLYISFGVTGMWAILTVVVVFEFTVGGTLSKG 97

Query: 117 VNRVIGTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTRFDYG 176
           +NR   T +AG LG+G   +A   G + EPI+LGI +F L +AATFSRF P +K R+DYG
Sbjct: 98  LNRGFATLIAGALGVGAVHLARFFGHQGEPIVLGILVFSLGAAATFSRFFPRIKQRYDYG 157

Query: 177 TMIFILTFCLVSISGYRVDKLFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLHSLIS 236
            +IFILTF  V+ISGYR D++  +A  RLSTI IG ++CI VS+  CP+WAG  LH +I+
Sbjct: 158 ALIFILTFSFVAISGYRTDEILIMAYQRLSTILIGGTICILVSIFICPVWAGEDLHKMIA 217

Query: 237 RNLDKLAHALDGCVSEYFKNDDDPEIKNEDQEGHDSKVEGYKCVLNSKASEESMANFARW 296
            N++KLA  L+G   EYF+    PE  +++     S V  YK +L SK++E+S+AN ARW
Sbjct: 218 NNINKLAKYLEGFEGEYFQ----PEKISKET---SSCVREYKSILTSKSTEDSLANLARW 277

Query: 297 EPAHGRFSFRHPWKKYLEIGVVMRKCAYCIEALHGCLNSEIKAPDSLKMHLAEPCKALSS 356
           EP HGRF  RHPWKKYL+I  ++R+CA  +E L+G + S  KAP   +  + EP   +S 
Sbjct: 278 EPGHGRFRLRHPWKKYLKIAGLVRQCAVHLEILNGYVLSNDKAPQEFESKIQEPITTMSR 337

Query: 357 SSSKVLKELSVVIKKMKKSTKIDLEVSDMNIAVQELQNAIKSLPSPHMGVSSAEPEGANQ 416
              + LK ++  IK M+  +      + +N  +   + AIK+L    + + S+ PE    
Sbjct: 338 EVGEALKAIAKSIKTMRNDS------ACVNAHIDNSKKAIKNL---KIALKSSYPE---- 397

Query: 417 DPKPTNPSLMELLPLATLVSLLIETVSRIEHVVNAVEALADVANFDTEEEKK 469
               T   L+E++P  T+ S+LIE V+ +E +  AVE  + +A+F    + K
Sbjct: 398 ----TYKDLLEIIPGVTMASILIEVVNCVEKIYEAVEEFSGLAHFKETLDSK 425

BLAST of Spg034543 vs. TAIR 10
Match: AT2G27240.1 (Aluminium activated malate transporter family protein )

HSP 1 Score: 321.6 bits (823), Expect = 1.1e-87
Identity = 180/437 (41.19%), Postives = 259/437 (59.27%), Query Frame = 0

Query: 56  IANSEPKKAVHGLKVGLALTLVSIFYYMRPLYEGVGGNAMWAIMTVVVTFESTVGATFYK 115
           +A  +P++ VH  KVGL L LVS FYY +PLY+  G NAMWA+MTVVV FE +VGAT  K
Sbjct: 14  VAKEDPRRVVHSFKVGLVLALVSSFYYYQPLYDSFGVNAMWAVMTVVVVFEFSVGATLGK 73

Query: 116 CVNRVIGTSLAGCLGIGVHWVAAESGDRFEPIILGISLFLLASAATFSRFIPSVKTRFDY 175
            +NRV  T  AG LGIG H +A+ SG   EPI+L + +F+ A+ +TF RF P VK R+DY
Sbjct: 74  GLNRVAATLFAGGLGIGAHHLASMSGPTGEPILLAVFVFVQAALSTFVRFFPRVKARYDY 133

Query: 176 GTMIFILTFCLVSISGYRVDKLFELARTRLSTIAIGTSLCIFVSMLFCPMWAGSQLHSLI 235
             +IFILTF L+S+SG+R +++ +L   R+ST+ IG   C+ +S+  CP+WAG  LHSLI
Sbjct: 134 SLLIFILTFALISVSGFREEQVVKLTHKRISTVIIGGLSCVIISIFVCPVWAGQDLHSLI 193

Query: 236 SRNLDKLAHALDGCVSEYFKND--------------------------DDPEIKNEDQEG 295
           + N +KL+  L G    Y  +D                          ++   K  D+  
Sbjct: 194 ASNFEKLSFFLLGNSFHYVSSDLNSITLLRKIKSWRLADFGDKYCEVVENDGAKEVDKRK 253

Query: 296 HDSKVEGYKCVLNSKASEESMANFARWEPAHGRFSFRHPWKKYLEIGVVMRKCAYCIEAL 355
            D   + YK VLNSK++EES+ANFA+WEP HG+F FRHPWK+YL +G ++R+CAY I AL
Sbjct: 254 KD--FDNYKSVLNSKSNEESLANFAKWEPGHGQFRFRHPWKQYLAVGELIRQCAYRIHAL 313

Query: 356 HGCLNSEIKAPDSLKMHLAEPCKALSSSSSKVLKELSVVIKKMKKSTKIDLEVSDMNIAV 415
           +  LN++ +    +K  L EP + +S  S K +KE+S+ +KKM K +  DL V +   A 
Sbjct: 314 NSYLNADNQVSVDIKKKLGEPLRRMSLESGKAMKEMSISLKKMTKPSSSDLHVQNAKSAC 373

Query: 416 QELQNAIKSLPSPHMGVSSAEPEGANQDPKPTNPSLMELLPLATLVSLLIETVSRIEHVV 467
           + L N + S              G  ++ +P     +EL+ L T +SLLI+ ++  E ++
Sbjct: 374 KSLTNLLNS--------------GILKEVEP-----LELVSLLTAISLLIDIINLTEKIL 429

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038906852.12.8e-23184.22aluminum-activated malate transporter 10 [Benincasa hispida][more]
XP_011658401.11.6e-22682.46aluminum-activated malate transporter 10 [Cucumis sativus] >KGN46529.1 hypotheti... [more]
XP_008459754.11.4e-22582.53PREDICTED: aluminum-activated malate transporter 10 [Cucumis melo][more]
XP_022960200.15.1e-22582.96aluminum-activated malate transporter 10 [Cucurbita moschata][more]
XP_023513501.18.8e-22582.75aluminum-activated malate transporter 10 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
O230861.9e-14556.24Aluminum-activated malate transporter 10 OS=Arabidopsis thaliana OX=3702 GN=ALMT... [more]
Q9SJE81.0e-9544.87Aluminum-activated malate transporter 2 OS=Arabidopsis thaliana OX=3702 GN=ALMT2... [more]
Q9SJE98.0e-8841.95Aluminum-activated malate transporter 1 OS=Arabidopsis thaliana OX=3702 GN=ALMT1... [more]
Q9SRM91.4e-8743.93Aluminum-activated malate transporter 8 OS=Arabidopsis thaliana OX=3702 GN=ALMT8... [more]
Q9XIN11.5e-8641.19Aluminum-activated malate transporter 7 OS=Arabidopsis thaliana OX=3702 GN=ALMT7... [more]
Match NameE-valueIdentityDescription
A0A0A0KCJ27.7e-22782.46Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G106810 PE=3 SV=1[more]
A0A1S3CB026.6e-22682.53aluminum-activated malate transporter 10 OS=Cucumis melo OX=3656 GN=LOC103498794... [more]
A0A6J1H8F32.5e-22582.96aluminum-activated malate transporter 10 OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1KS061.2e-22282.34aluminum-activated malate transporter 10 OS=Cucurbita maxima OX=3661 GN=LOC11149... [more]
A0A6J1DX419.2e-20476.78aluminum-activated malate transporter 10 isoform X1 OS=Momordica charantia OX=36... [more]
Match NameE-valueIdentityDescription
AT4G00910.11.3e-14656.24Aluminium activated malate transporter family protein [more]
AT1G08440.17.4e-9744.87Aluminium activated malate transporter family protein [more]
AT1G08430.15.7e-8941.95aluminum-activated malate transporter 1 [more]
AT3G11680.19.7e-8943.93Aluminium activated malate transporter family protein [more]
AT2G27240.11.1e-8741.19Aluminium activated malate transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020966Aluminum-activated malate transporterPFAMPF11744ALMTcoord: 412..456
e-value: 4.2E-5
score: 22.6
coord: 54..400
e-value: 4.3E-154
score: 513.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 400..424
NoneNo IPR availablePANTHERPTHR31086ALUMINUM-ACTIVATED MALATE TRANSPORTER 10coord: 5..472
NoneNo IPR availablePANTHERPTHR31086:SF4ALUMINUM ACTIVATED MALATE TRANSPORTER FAMILY PROTEINcoord: 5..472

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg034543.1Spg034543.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034220 ion transmembrane transport
biological_process GO:0015743 malate transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009705 plant-type vacuole membrane