Spg034331 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg034331
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Hydroxymethylbilane hydrolyase [cyclizing]
Location	scaffold12: 21155910 .. 21156452 (+)
RNA-Seq Expression	Spg034331
Synteny	Spg034331

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCCGGACAAGGGAGTGTGCGAGGAAGCTTGTGGAGAGAAGGGAGGATGGAAAATTGGATGCCATTGTGTTTACAAGTACTCGGGAACTGGAGGGGCTGGTTAAAAGCTTGAGTGATTTGGGATTAGAGTGGGAGATGATGAGAAAGAGATGGTCGGAAACGGTGGTGGCCACGCATGGGTTGATAACGGTGGTGGGAGCAGAGAGTCTCAGAATCAAGGTTGACTTGGTGAGTTCTCTAAATTTGATAGTTTCAATGGTGTGATTGATGTTTTTCATTCGAGATGGTAGAGCTTAGAACAGAACCCTGTGTAAGTGCATGAACATTCTTAAATATGCCATTGTGTTTACAAGTACTCGGGAACTGGAGGGGCTGGTTAAAAGCTTGAGTGATTTGGGATTAGAGTGGGAGATGATGAGAAAGAGATGGTCGGAAACGGTGGTGGCCACGCATGGGTTGATAACGGTGGTGGGAGCAGAGAGTCTCAGAATCAAGGTTGACTTGGTGAGTTCTCTAAATTTGATAGTTTCAATGGTGTGA

mRNA sequence

ATGGGCCGGACAAGGGAGTGTGCGAGGAAGCTTGTGGAGAGAAGGGAGGATGGAAAATTGGATGCCATTGTGTTTACAAGTACTCGGGAACTGGAGGGGCTGGTTAAAAGCTTGAGTGATTTGGGATTAGAGTGGGAGATGATGAGAAAGAGATGGTCGGAAACGGTGGTGGCCACGCATGGGTTGATAACGGTGGTGGGAGCAGAGAGTCTCAGAATCAAGGTTGACTTGTGCATGAACATTCTTAAATATGCCATTGTGTTTACAAGTACTCGGGAACTGGAGGGGCTGGTTAAAAGCTTGAGTGATTTGGGATTAGAGTGGGAGATGATGAGAAAGAGATGGTCGGAAACGGTGGTGGCCACGCATGGGTTGATAACGGTGGTGGGAGCAGAGAGTCTCAGAATCAAGGTTGACTTGGTGAGTTCTCTAAATTTGATAGTTTCAATGGTGTGA

Coding sequence (CDS)

ATGGGCCGGACAAGGGAGTGTGCGAGGAAGCTTGTGGAGAGAAGGGAGGATGGAAAATTGGATGCCATTGTGTTTACAAGTACTCGGGAACTGGAGGGGCTGGTTAAAAGCTTGAGTGATTTGGGATTAGAGTGGGAGATGATGAGAAAGAGATGGTCGGAAACGGTGGTGGCCACGCATGGGTTGATAACGGTGGTGGGAGCAGAGAGTCTCAGAATCAAGGTTGACTTGTGCATGAACATTCTTAAATATGCCATTGTGTTTACAAGTACTCGGGAACTGGAGGGGCTGGTTAAAAGCTTGAGTGATTTGGGATTAGAGTGGGAGATGATGAGAAAGAGATGGTCGGAAACGGTGGTGGCCACGCATGGGTTGATAACGGTGGTGGGAGCAGAGAGTCTCAGAATCAAGGTTGACTTGGTGAGTTCTCTAAATTTGATAGTTTCAATGGTGTGA

Protein sequence

MGRTRECARKLVERREDGKLDAIVFTSTRELEGLVKSLSDLGLEWEMMRKRWSETVVATHGLITVVGAESLRIKVDLCMNILKYAIVFTSTRELEGLVKSLSDLGLEWEMMRKRWSETVVATHGLITVVGAESLRIKVDLVSSLNLIVSMV

Homology

[Close]

BLAST of Spg034331 vs. NCBI nr
Match: XP_008444040.1 (PREDICTED: uncharacterized protein LOC103487492 [Cucumis melo] >KAA0050031.1 Tetrapyrrole biosynthesis, uroporphyrinogen III synthase [Cucumis melo var. makuwa])

HSP 1 Score: 105.1 bits (261), Expect = 5.2e-19
Identity = 51/72 (70.83%), Postives = 59/72 (81.94%), Query Frame = 0


Query: 6   ECARKLVERREDGKLDAIVFTSTRELEGLVKSLSDLGLEWEMMRKRWSETVVATHGLITV 65
           +CARKLVER +D KLDAIVFTST E+EGL+KSL  LGLEW+MM+KRW E VVA HG +T 
Sbjct: 213 DCARKLVERGKDEKLDAIVFTSTGEVEGLLKSLEHLGLEWDMMKKRWPEMVVAAHGPVTA 272

Query: 66  VGAESLRIKVDL 78
            GAE L +KVDL
Sbjct: 273 AGAERLGVKVDL 284

[Close]

BLAST of Spg034331 vs. NCBI nr
Match: XP_023530963.1 (uncharacterized protein LOC111793350 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 104.0 bits (258), Expect = 1.2e-18
Identity = 50/72 (69.44%), Postives = 58/72 (80.56%), Query Frame = 0


Query: 6   ECARKLVERREDGKLDAIVFTSTRELEGLVKSLSDLGLEWEMMRKRWSETVVATHGLITV 65
           ECARKL ER ED KLDAIVFTST E+EGL+KSL  LGL+WEMMRK+W E V+A HG +T 
Sbjct: 213 ECARKLAERGEDEKLDAIVFTSTGEVEGLLKSLRALGLQWEMMRKKWPEMVLAAHGPVTA 272

Query: 66  VGAESLRIKVDL 78
            GAE L +K+DL
Sbjct: 273 AGAERLGVKIDL 284

[Close]

BLAST of Spg034331 vs. NCBI nr
Match: KAG6587847.1 (hypothetical protein SDJN03_16412, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035661.1 hypothetical protein SDJN02_02459, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 103.6 bits (257), Expect = 1.5e-18
Identity = 50/72 (69.44%), Postives = 58/72 (80.56%), Query Frame = 0


Query: 6   ECARKLVERREDGKLDAIVFTSTRELEGLVKSLSDLGLEWEMMRKRWSETVVATHGLITV 65
           ECARKL ER ED KLDAIVFTST E+EGL+KSL  LGL+WEMMRK+W E V+A HG +T 
Sbjct: 213 ECARKLAERGEDEKLDAIVFTSTGEVEGLLKSLRALGLKWEMMRKKWPEMVLAAHGPVTA 272

Query: 66  VGAESLRIKVDL 78
            GAE L +K+DL
Sbjct: 273 AGAERLGVKIDL 284

[Close]

BLAST of Spg034331 vs. NCBI nr
Match: XP_022926996.1 (uncharacterized protein LOC111433956 [Cucurbita moschata])

HSP 1 Score: 103.6 bits (257), Expect = 1.5e-18
Identity = 50/72 (69.44%), Postives = 58/72 (80.56%), Query Frame = 0


Query: 6   ECARKLVERREDGKLDAIVFTSTRELEGLVKSLSDLGLEWEMMRKRWSETVVATHGLITV 65
           ECARKL ER ED KLDAIVFTST E+EGL+KSL  LGL+WEMMRK+W E V+A HG +T 
Sbjct: 213 ECARKLAERGEDEKLDAIVFTSTGEVEGLLKSLRALGLKWEMMRKKWPEMVLAAHGPVTA 272

Query: 66  VGAESLRIKVDL 78
            GAE L +K+DL
Sbjct: 273 AGAERLGVKIDL 284

[Close]

BLAST of Spg034331 vs. NCBI nr
Match: TYK03635.1 (Tetrapyrrole biosynthesis, uroporphyrinogen III synthase [Cucumis melo var. makuwa])

HSP 1 Score: 103.6 bits (257), Expect = 1.5e-18
Identity = 50/72 (69.44%), Postives = 59/72 (81.94%), Query Frame = 0


Query: 6   ECARKLVERREDGKLDAIVFTSTRELEGLVKSLSDLGLEWEMMRKRWSETVVATHGLITV 65
           +CARKLVER +D KLDAIVFTST E+EGL+KSL  LGLEW++M+KRW E VVA HG +T 
Sbjct: 213 DCARKLVERGKDEKLDAIVFTSTGEVEGLLKSLEHLGLEWDIMKKRWPEMVVAAHGPVTA 272

Query: 66  VGAESLRIKVDL 78
            GAE L +KVDL
Sbjct: 273 AGAERLGVKVDL 284

[Close]

BLAST of Spg034331 vs. ExPASy TrEMBL
Match: A0A5A7U8V3 (Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold675G00050 PE=3 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 2.5e-19
Identity = 51/72 (70.83%), Postives = 59/72 (81.94%), Query Frame = 0


Query: 6   ECARKLVERREDGKLDAIVFTSTRELEGLVKSLSDLGLEWEMMRKRWSETVVATHGLITV 65
           +CARKLVER +D KLDAIVFTST E+EGL+KSL  LGLEW+MM+KRW E VVA HG +T 
Sbjct: 213 DCARKLVERGKDEKLDAIVFTSTGEVEGLLKSLEHLGLEWDMMKKRWPEMVVAAHGPVTA 272

Query: 66  VGAESLRIKVDL 78
            GAE L +KVDL
Sbjct: 273 AGAERLGVKVDL 284

[Close]

BLAST of Spg034331 vs. ExPASy TrEMBL
Match: A0A1S3BA78 (Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucumis melo OX=3656 GN=LOC103487492 PE=3 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 2.5e-19
Identity = 51/72 (70.83%), Postives = 59/72 (81.94%), Query Frame = 0


Query: 6   ECARKLVERREDGKLDAIVFTSTRELEGLVKSLSDLGLEWEMMRKRWSETVVATHGLITV 65
           +CARKLVER +D KLDAIVFTST E+EGL+KSL  LGLEW+MM+KRW E VVA HG +T 
Sbjct: 213 DCARKLVERGKDEKLDAIVFTSTGEVEGLLKSLEHLGLEWDMMKKRWPEMVVAAHGPVTA 272

Query: 66  VGAESLRIKVDL 78
            GAE L +KVDL
Sbjct: 273 AGAERLGVKVDL 284

[Close]

BLAST of Spg034331 vs. ExPASy TrEMBL
Match: A0A5D3BX13 (Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1369G00630 PE=3 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 7.4e-19
Identity = 50/72 (69.44%), Postives = 59/72 (81.94%), Query Frame = 0


Query: 6   ECARKLVERREDGKLDAIVFTSTRELEGLVKSLSDLGLEWEMMRKRWSETVVATHGLITV 65
           +CARKLVER +D KLDAIVFTST E+EGL+KSL  LGLEW++M+KRW E VVA HG +T 
Sbjct: 213 DCARKLVERGKDEKLDAIVFTSTGEVEGLLKSLEHLGLEWDIMKKRWPEMVVAAHGPVTA 272

Query: 66  VGAESLRIKVDL 78
            GAE L +KVDL
Sbjct: 273 AGAERLGVKVDL 284

[Close]

BLAST of Spg034331 vs. ExPASy TrEMBL
Match: A0A6J1EGG3 (Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucurbita moschata OX=3662 GN=LOC111433956 PE=3 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 7.4e-19
Identity = 50/72 (69.44%), Postives = 58/72 (80.56%), Query Frame = 0


Query: 6   ECARKLVERREDGKLDAIVFTSTRELEGLVKSLSDLGLEWEMMRKRWSETVVATHGLITV 65
           ECARKL ER ED KLDAIVFTST E+EGL+KSL  LGL+WEMMRK+W E V+A HG +T 
Sbjct: 213 ECARKLAERGEDEKLDAIVFTSTGEVEGLLKSLRALGLKWEMMRKKWPEMVLAAHGPVTA 272

Query: 66  VGAESLRIKVDL 78
            GAE L +K+DL
Sbjct: 273 AGAERLGVKIDL 284

[Close]

BLAST of Spg034331 vs. ExPASy TrEMBL
Match: A0A0A0LZ08 (Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucumis sativus OX=3659 GN=Csa_1G266180 PE=3 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 1.3e-18
Identity = 49/72 (68.06%), Postives = 60/72 (83.33%), Query Frame = 0


Query: 6   ECARKLVERREDGKLDAIVFTSTRELEGLVKSLSDLGLEWEMMRKRWSETVVATHGLITV 65
           +CARKLVER +D KLDAIVFTST E+EGL+KSL+ LGLEW++M++RW E VVA HG +T 
Sbjct: 213 DCARKLVERGKDEKLDAIVFTSTGEVEGLLKSLAHLGLEWDVMKRRWPEMVVAAHGPVTA 272

Query: 66  VGAESLRIKVDL 78
            GAE L +KVDL
Sbjct: 273 AGAERLGVKVDL 284

The following BLAST results are available for this feature:

BLAST of Spg034331 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008444040.1	5.2e-19	70.83	PREDICTED: uncharacterized protein LOC103487492 [Cucumis melo] >KAA0050031.1 Tet...	[more]
XP_023530963.1	1.2e-18	69.44	uncharacterized protein LOC111793350 [Cucurbita pepo subsp. pepo]	[more]
KAG6587847.1	1.5e-18	69.44	hypothetical protein SDJN03_16412, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022926996.1	1.5e-18	69.44	uncharacterized protein LOC111433956 [Cucurbita moschata]	[more]
TYK03635.1	1.5e-18	69.44	Tetrapyrrole biosynthesis, uroporphyrinogen III synthase [Cucumis melo var. maku...	[more]

BLAST of Spg034331 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Spg034331 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7U8V3	2.5e-19	70.83	Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A1S3BA78	2.5e-19	70.83	Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucumis melo OX=3656 GN=LOC1034874...	[more]
A0A5D3BX13	7.4e-19	69.44	Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A6J1EGG3	7.4e-19	69.44	Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A0A0LZ08	1.3e-18	68.06	Hydroxymethylbilane hydrolyase [cyclizing] OS=Cucumis sativus OX=3659 GN=Csa_1G2...	[more]

BLAST of Spg034331 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036108	Tetrapyrrole biosynthesis, uroporphyrinogen III synthase superfamily	GENE3D	3.40.50.10090		coord: 3..80 e-value: 1.2E-5 score: 27.0
IPR036108	Tetrapyrrole biosynthesis, uroporphyrinogen III synthase superfamily	SUPERFAMILY	69618	HemD-like	coord: 8..78
None	No IPR available	PANTHER	PTHR38020	UROPORPHYRINOGEN-III SYNTHASE	coord: 5..77 coord: 85..143

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg034331.1	Spg034331.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006782	protoporphyrinogen IX biosynthetic process
biological_process	GO:0033014	tetrapyrrole biosynthetic process
molecular_function	GO:0004852	uroporphyrinogen-III synthase activity