Spg033528 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg033528
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionMethyltransf_11 domain-containing protein
Locationscaffold5: 2648806 .. 2650503 (+)
RNA-Seq ExpressionSpg033528
SyntenySpg033528
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGACTTGGGACGGGAGAATTCGGAGGATGAGGGTTCCGAAGAGGGTCTGGTTAATTTTACAAAAATCTATGGGGCTCCTCGTGGGTGGAAATCACACATCGTCCAGGTTTTGTACTCGCTAATGTTGTTTTGGTTTCTCAATTTGGCTTTGTTTCTCTGTGTTGTGTTGTTTTGAGGAAAGTATTCCTGTTTTGTTCGTCTTTGACGACGATAAACGAACTCTGAGCGGGTTGGGATTGGCGATTGGAGAGATGAGTTTGAAGGCTGTGAAATGGCAGATCGTTCATGGGGCAATGGCGAGGCGTGTTGTCGTTCGAACTTTTATTTTCGCCTTAGCTGTTTCGACTGTTCCTTTGTTGCATATTTTGATGGGTGCGGATTTTGGAGTGATTCCGTCGGTGATCTTTCGCGATTGTGTTGTGATGTCTGATGATTCGAATGTGAATGCGAAAGTTTCTCGAGGTTCGTACTTGTTTCGAGGTCACTTTCTGAATCCGATTTGGGTGCCATTTGTAGCGGTACACTGTGAAGAACATATGAATCTGACGACTAATGTCGTTACTGAGTTAATAGAGAAGAAACTGTTGAACCACAGTGCGAAATCTCTCTGCGTTGGAGAGGGATCGGGCTCCGCCGTTCTGGCACTGAGGGACATGGGATTCAGTGATGTTATGGGTGTTGGTCAACACCGATTTTTCTCGTTAAGGAGAAAACAATTCGTTTATGAACTGGACTTTGAGGATCGATCCTTTGATTTCGTTTTCTCTAGAGATCTAGACAGGTATTCAGTACCTGCACTTTTGGTGCTCGAGATCGAGCGTGTGCTTAAACCTGGCGGAATTGGGGCTGTCATTGTGGGTACAAGTGGTTCAATGCCCAATAATTTGATTAGAGCTGCTACCCCAGTGTCATCTTTGCTGAAAACTTCCACTGTGATGCATGTTGGCCATGTTAATAACTTAACTCTGGTTGTATTTAAGAAGAAACCTGAAGAATTTCACCAATTTGAGCGTCGCCTGTCGTCTGAATGTCGCTCTCTCACAAGGAACAGACCTTTAATTCAAAAAATGGAGCCTCTTGTGAAGGAAAAACCTGTGGGATTTGACGAAAAGCTATCTTATTTGCCGAAGCTCGTAGCTGCTTCCAATGGGGAAAAGTTGGTATATGTTAATATTGGTACAGGGAAGCGCCTGAATAGCACCGACACAGATTGGTTTCCACCCTCTTATCCTGTGGATCGCAGAGACTTCAATGTTTATTTTGTTGATCATGACATGTCTGCTCTCGCCACCCATATCCACAATCCGGGAGTCACGTTTGTTTACCATCCTGGCCTGGCTGGAACTGATCAGACAACAGATAACGACGGCAGTGCCGACGACGAAGACGAAGAACCATACGTTGACGACGAGTTTGATTTCCTGTCTTGGTTCAAGGAAACTGTGCAGTATGCCGATTTCGTCGTCTTGAAAATGGATGCAGGGAAGGAAGAACTGAAGTTTCTATCAGATTTGTTCGAAAGTGGGATTATTTGCTGGGTGGACGAGGTGTTTCTGAGCTGCAGAGATGATGGGGTTGATGAAGATGGTGAGCTGAAAAGAGACTGTATGGATCTGTTCAACGATTTGAGAAACAATGGTGTCTATGTCCATCAATGGTTTCTGGATGCTCCTTCCACCGACAATGTGAAGTAG

mRNA sequence

ATGGGGACTTGGGACGGGAGAATTCGGAGGATGAGGGTTCCGAAGAGGGTCTGGTTAATTTTACAAAAATCTATGGGGCTCCTCGTGGGTGGAAATCACACATCGTCCAGTATTCCTGTTTTGTTCGTCTTTGACGACGATAAACGAACTCTGAGCGGGTTGGGATTGGCGATTGGAGAGATGAGTTTGAAGGCTGTGAAATGGCAGATCGTTCATGGGGCAATGGCGAGGCGTGTTGTCGTTCGAACTTTTATTTTCGCCTTAGCTGTTTCGACTGTTCCTTTGTTGCATATTTTGATGGGTGCGGATTTTGGAGTGATTCCGTCGGTGATCTTTCGCGATTGTGTTGTGATGTCTGATGATTCGAATGTGAATGCGAAAGTTTCTCGAGGTTCGTACTTGTTTCGAGGTCACTTTCTGAATCCGATTTGGGTGCCATTTGTAGCGGTACACTGTGAAGAACATATGAATCTGACGACTAATGTCGTTACTGAGTTAATAGAGAAGAAACTGTTGAACCACAGTGCGAAATCTCTCTGCGTTGGAGAGGGATCGGGCTCCGCCGTTCTGGCACTGAGGGACATGGGATTCAGTGATGTTATGGGTGTTGGTCAACACCGATTTTTCTCGTTAAGGAGAAAACAATTCGTTTATGAACTGGACTTTGAGGATCGATCCTTTGATTTCGTTTTCTCTAGAGATCTAGACAGGTATTCAGTACCTGCACTTTTGGTGCTCGAGATCGAGCGTGTGCTTAAACCTGGCGGAATTGGGGCTGTCATTGTGGGTACAAGTGGTTCAATGCCCAATAATTTGATTAGAGCTGCTACCCCAGTGTCATCTTTGCTGAAAACTTCCACTGTGATGCATGTTGGCCATGTTAATAACTTAACTCTGGTTGTATTTAAGAAGAAACCTGAAGAATTTCACCAATTTGAGCGTCGCCTGTCGTCTGAATGTCGCTCTCTCACAAGGAACAGACCTTTAATTCAAAAAATGGAGCCTCTTGTGAAGGAAAAACCTGTGGGATTTGACGAAAAGCTATCTTATTTGCCGAAGCTCGTAGCTGCTTCCAATGGGGAAAAGTTGGTATATGTTAATATTGGTACAGGGAAGCGCCTGAATAGCACCGACACAGATTGGTTTCCACCCTCTTATCCTGTGGATCGCAGAGACTTCAATGTTTATTTTGTTGATCATGACATGTCTGCTCTCGCCACCCATATCCACAATCCGGGAGTCACGTTTGTTTACCATCCTGGCCTGGCTGGAACTGATCAGACAACAGATAACGACGGCAGTGCCGACGACGAAGACGAAGAACCATACGTTGACGACGAGTTTGATTTCCTGTCTTGGTTCAAGGAAACTGTGCAGTATGCCGATTTCGTCGTCTTGAAAATGGATGCAGGGAAGGAAGAACTGAAGTTTCTATCAGATTTGTTCGAAAGTGGGATTATTTGCTGGGTGGACGAGGTGTTTCTGAGCTGCAGAGATGATGGGGTTGATGAAGATGGTGAGCTGAAAAGAGACTGTATGGATCTGTTCAACGATTTGAGAAACAATGGTGTCTATGTCCATCAATGGTTTCTGGATGCTCCTTCCACCGACAATGTGAAGTAG

Coding sequence (CDS)

ATGGGGACTTGGGACGGGAGAATTCGGAGGATGAGGGTTCCGAAGAGGGTCTGGTTAATTTTACAAAAATCTATGGGGCTCCTCGTGGGTGGAAATCACACATCGTCCAGTATTCCTGTTTTGTTCGTCTTTGACGACGATAAACGAACTCTGAGCGGGTTGGGATTGGCGATTGGAGAGATGAGTTTGAAGGCTGTGAAATGGCAGATCGTTCATGGGGCAATGGCGAGGCGTGTTGTCGTTCGAACTTTTATTTTCGCCTTAGCTGTTTCGACTGTTCCTTTGTTGCATATTTTGATGGGTGCGGATTTTGGAGTGATTCCGTCGGTGATCTTTCGCGATTGTGTTGTGATGTCTGATGATTCGAATGTGAATGCGAAAGTTTCTCGAGGTTCGTACTTGTTTCGAGGTCACTTTCTGAATCCGATTTGGGTGCCATTTGTAGCGGTACACTGTGAAGAACATATGAATCTGACGACTAATGTCGTTACTGAGTTAATAGAGAAGAAACTGTTGAACCACAGTGCGAAATCTCTCTGCGTTGGAGAGGGATCGGGCTCCGCCGTTCTGGCACTGAGGGACATGGGATTCAGTGATGTTATGGGTGTTGGTCAACACCGATTTTTCTCGTTAAGGAGAAAACAATTCGTTTATGAACTGGACTTTGAGGATCGATCCTTTGATTTCGTTTTCTCTAGAGATCTAGACAGGTATTCAGTACCTGCACTTTTGGTGCTCGAGATCGAGCGTGTGCTTAAACCTGGCGGAATTGGGGCTGTCATTGTGGGTACAAGTGGTTCAATGCCCAATAATTTGATTAGAGCTGCTACCCCAGTGTCATCTTTGCTGAAAACTTCCACTGTGATGCATGTTGGCCATGTTAATAACTTAACTCTGGTTGTATTTAAGAAGAAACCTGAAGAATTTCACCAATTTGAGCGTCGCCTGTCGTCTGAATGTCGCTCTCTCACAAGGAACAGACCTTTAATTCAAAAAATGGAGCCTCTTGTGAAGGAAAAACCTGTGGGATTTGACGAAAAGCTATCTTATTTGCCGAAGCTCGTAGCTGCTTCCAATGGGGAAAAGTTGGTATATGTTAATATTGGTACAGGGAAGCGCCTGAATAGCACCGACACAGATTGGTTTCCACCCTCTTATCCTGTGGATCGCAGAGACTTCAATGTTTATTTTGTTGATCATGACATGTCTGCTCTCGCCACCCATATCCACAATCCGGGAGTCACGTTTGTTTACCATCCTGGCCTGGCTGGAACTGATCAGACAACAGATAACGACGGCAGTGCCGACGACGAAGACGAAGAACCATACGTTGACGACGAGTTTGATTTCCTGTCTTGGTTCAAGGAAACTGTGCAGTATGCCGATTTCGTCGTCTTGAAAATGGATGCAGGGAAGGAAGAACTGAAGTTTCTATCAGATTTGTTCGAAAGTGGGATTATTTGCTGGGTGGACGAGGTGTTTCTGAGCTGCAGAGATGATGGGGTTGATGAAGATGGTGAGCTGAAAAGAGACTGTATGGATCTGTTCAACGATTTGAGAAACAATGGTGTCTATGTCCATCAATGGTTTCTGGATGCTCCTTCCACCGACAATGTGAAGTAG

Protein sequence

MGTWDGRIRRMRVPKRVWLILQKSMGLLVGGNHTSSSIPVLFVFDDDKRTLSGLGLAIGEMSLKAVKWQIVHGAMARRVVVRTFIFALAVSTVPLLHILMGADFGVIPSVIFRDCVVMSDDSNVNAKVSRGSYLFRGHFLNPIWVPFVAVHCEEHMNLTTNVVTELIEKKLLNHSAKSLCVGEGSGSAVLALRDMGFSDVMGVGQHRFFSLRRKQFVYELDFEDRSFDFVFSRDLDRYSVPALLVLEIERVLKPGGIGAVIVGTSGSMPNNLIRAATPVSSLLKTSTVMHVGHVNNLTLVVFKKKPEEFHQFERRLSSECRSLTRNRPLIQKMEPLVKEKPVGFDEKLSYLPKLVAASNGEKLVYVNIGTGKRLNSTDTDWFPPSYPVDRRDFNVYFVDHDMSALATHIHNPGVTFVYHPGLAGTDQTTDNDGSADDEDEEPYVDDEFDFLSWFKETVQYADFVVLKMDAGKEELKFLSDLFESGIICWVDEVFLSCRDDGVDEDGELKRDCMDLFNDLRNNGVYVHQWFLDAPSTDNVK
Homology
BLAST of Spg033528 vs. NCBI nr
Match: XP_038904424.1 (uncharacterized protein LOC120090789 [Benincasa hispida])

HSP 1 Score: 815.1 bits (2104), Expect = 3.7e-232
Identity = 406/480 (84.58%), Postives = 437/480 (91.04%), Query Frame = 0

Query: 61  MSLKAVKWQIVHGAMARRVVVRTFIFALAVSTVPLLHILMGADFGVIPSVIFRDCVVMSD 120
           M+LKAVKWQIVHG +ARRVVVR F  ALAVSTVPLLHIL GADFGVIPSVIFRDC V S 
Sbjct: 1   MNLKAVKWQIVHGVLARRVVVRIFFLALAVSTVPLLHILTGADFGVIPSVIFRDCAVKS- 60

Query: 121 DSNVNAKVSRGSYLFRGHFLNPIWVPFVAVHCEEHMNLTTNVVTELIEKKLLNHSAKSLC 180
              V AK SRGSYLF+GHFLNPIWVPF A+HCE+ MNLTTNVV EL+EKKLLNH+AK LC
Sbjct: 61  -GYVEAKDSRGSYLFQGHFLNPIWVPFAAMHCEDSMNLTTNVVAELMEKKLLNHTAKCLC 120

Query: 181 VGEGSGSAVLALRDMGFSDVMGVGQHRFFSLRRKQFVYELDFEDRSFDFVFSRDLDRYSV 240
           VGEGSGSAVLALRD+GFSDV+GVGQHRFFSLRRKQ VYELDF+D SFDFVFSRDLDRYSV
Sbjct: 121 VGEGSGSAVLALRDIGFSDVIGVGQHRFFSLRRKQLVYELDFKDGSFDFVFSRDLDRYSV 180

Query: 241 PALLVLEIERVLKPGGIGAVIVGTSGSMPNNLIRAATPVSSLLKTSTVMHVGHVNNLTLV 300
           PALLVLEIERVL+PGGIGAVIV TSGSMPNNLIRAATPVSSLLKTSTVMHVGHVNNLTLV
Sbjct: 181 PALLVLEIERVLRPGGIGAVIVATSGSMPNNLIRAATPVSSLLKTSTVMHVGHVNNLTLV 240

Query: 301 VFKKKPEEFHQFERRLSSECRSLTRNRPLIQKMEPLVKEKPVGFDEKLSYLPKLVAASNG 360
           VFKKK EEF   E  +SSECRSLTRN+PLI K+EP VK KPV FD+KLSYLPKLV  SNG
Sbjct: 241 VFKKKLEEFRHLEPHISSECRSLTRNKPLIPKIEPFVKGKPVEFDKKLSYLPKLVDVSNG 300

Query: 361 EKLVYVNIGTGKRLNSTDTDWFPPSYPVDRRDFNVYFVDHDMSALATHIHNPGVTFVYHP 420
           EKLVYVNIG GKR+N T+TDWFPPSYPVDRRDFNVYFVDHDMSALAT+IHNPGVTFVYHP
Sbjct: 301 EKLVYVNIGPGKRMNHTNTDWFPPSYPVDRRDFNVYFVDHDMSALATYIHNPGVTFVYHP 360

Query: 421 GLAGTDQTTDNDGSADDEDEEPYVDDEFDFLSWFKETVQYADFVVLKMDAGKEELKFLSD 480
           GLAGTDQTT+NDG+ADDEDEEPY+DDEFDFLSWFKETVQ++DFVVLKMDAGKEELKFLSD
Sbjct: 361 GLAGTDQTTENDGAADDEDEEPYIDDEFDFLSWFKETVQHSDFVVLKMDAGKEELKFLSD 420

Query: 481 LFESGIICWVDEVFLSCRDDGVDEDGELK-RDCMDLFNDLRNNGVYVHQWFLDAPSTDNV 540
           LFESG+ICWVDEVFLSCRD+  +EDGELK R C+DL+ DLRN+GVYVHQWFLDA  +  +
Sbjct: 421 LFESGVICWVDEVFLSCRDEVDEEDGELKNRGCVDLYKDLRNSGVYVHQWFLDAAPSKKI 478

BLAST of Spg033528 vs. NCBI nr
Match: XP_016899642.1 (PREDICTED: uncharacterized protein LOC107990602 [Cucumis melo] >KAA0044016.1 Methyltransferase type 11 [Cucumis melo var. makuwa] >TYK25124.1 Methyltransferase type 11 [Cucumis melo var. makuwa])

HSP 1 Score: 806.2 bits (2081), Expect = 1.7e-229
Identity = 398/474 (83.97%), Postives = 436/474 (91.98%), Query Frame = 0

Query: 61  MSLKAVKWQIVHGAMARRVVVRTFIFALAVSTVPLLHILMGADFGVIPSVIFRDCVVMSD 120
           M+LKAVKWQI+HGA+ARR+VVR F+ AL VS VPLLHI MGADFGVIPSVIFRDC V   
Sbjct: 1   MNLKAVKWQIIHGALARRIVVRIFLLALTVSAVPLLHIFMGADFGVIPSVIFRDCGVKYG 60

Query: 121 DSNVNAKVSRGSYLFRGHFLNPIWVPFVAVHCEEHMNLTTNVVTELIEKKLLNHSAKSLC 180
           D  + AKVSRGSY+F+GHFLNPIWVPFVA+HCEE+ NLTTNVV EL+EKKLLNHSAKSLC
Sbjct: 61  D--MEAKVSRGSYMFQGHFLNPIWVPFVAMHCEEYKNLTTNVVAELMEKKLLNHSAKSLC 120

Query: 181 VGEGSGSAVLALRDMGFSDVMGVGQHRFFSLRRKQFVYELDFEDRSFDFVFSRDLDRYSV 240
           VGEGSGSAVLALRD+GFSDV+GV QHRFFSLRRKQFVYELDF+   FDFVFSRDLDR+SV
Sbjct: 121 VGEGSGSAVLALRDIGFSDVIGVDQHRFFSLRRKQFVYELDFKSGYFDFVFSRDLDRHSV 180

Query: 241 PALLVLEIERVLKPGGIGAVIVGTSGSMPNNLIRAATPVSSLLKTSTVMHVGHVNNLTLV 300
           PALLVLEIERVL+PGGIGAVIV +SGSMPNNLIRAATPVSSLLKTSTVMHVGHVNNLTLV
Sbjct: 181 PALLVLEIERVLRPGGIGAVIVSSSGSMPNNLIRAATPVSSLLKTSTVMHVGHVNNLTLV 240

Query: 301 VFKKKPEEFHQFERRLSSECRSLTRNRPLIQKMEPLVKEKPVGFDEKLSYLPKLVAASNG 360
           VFKKK EE    ERR+SSECRSLTRN+PLI+K+EPLVKE PVGFD+KLSYLPK V  S+G
Sbjct: 241 VFKKKLEEHRHLERRMSSECRSLTRNKPLIRKLEPLVKEGPVGFDKKLSYLPKFVDVSSG 300

Query: 361 EKLVYVNIGTGKRLNSTDTDWFPPSYPVDRRDFNVYFVDHDMSALATHIHNPGVTFVYHP 420
           ++L+YVNIGTGKRLN T+TDWFPPSYPVDRRDFNVYFVDHDMS LATHIHNPGVTFVYHP
Sbjct: 301 KRLIYVNIGTGKRLNHTNTDWFPPSYPVDRRDFNVYFVDHDMSYLATHIHNPGVTFVYHP 360

Query: 421 GLAGTDQTTDNDGSADDEDEEPYVDDEFDFLSWFKETVQYADFVVLKMDAGKEELKFLSD 480
            LAG DQTTD+D +ADDEDEEPY+DDEFDFLSWFKETVQ++DFVVLKMDAGKEELKFLSD
Sbjct: 361 ALAGIDQTTDSDDAADDEDEEPYIDDEFDFLSWFKETVQHSDFVVLKMDAGKEELKFLSD 420

Query: 481 LFESGIICWVDEVFLSCRDDGVDEDGEL-KRDCMDLFNDLRNNGVYVHQWFLDA 534
           LFESG+ICWVDEVFLSCRD   +EDG+L KR+CMDL+ DLRN+GVYVHQWFLDA
Sbjct: 421 LFESGVICWVDEVFLSCRDGVDEEDGDLKKRECMDLYKDLRNSGVYVHQWFLDA 472

BLAST of Spg033528 vs. NCBI nr
Match: KAG6596469.1 (hypothetical protein SDJN03_09649, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 805.8 bits (2080), Expect = 2.2e-229
Identity = 401/477 (84.07%), Postives = 433/477 (90.78%), Query Frame = 0

Query: 61  MSLKAVKWQIVHGAMARRVVVRTFIFALAVSTVPLLHILMGADFGVIPSVIFRDCVVMSD 120
           MSLK +KWQIVHGA+ARRV+VRTF+FALAVS VPLLHIL GA+FG IPSVIFRDC V  D
Sbjct: 1   MSLKGMKWQIVHGALARRVIVRTFLFALAVSAVPLLHILTGANFGEIPSVIFRDCAVKCD 60

Query: 121 DSNVNAKVSRGSYLFRGHFLNPIWVPFVAVHCEEHMNLTTNVVTELIEKKLLNHSAKSLC 180
             N NAK SRGSYLF+GHFLNP+WVPFV +HCEEHMNLTTNVV EL+EKKLLNH  KSLC
Sbjct: 61  YVNSNAKDSRGSYLFQGHFLNPVWVPFVGMHCEEHMNLTTNVVIELMEKKLLNHGVKSLC 120

Query: 181 VGEGSGSAVLALRDMGFSDVMGVGQHRFFSLRRKQFVYELDFEDRSFDFVFSRDLDRYSV 240
           VGE S SAVLALRDMGFSDVMGVGQ  FFSLRRKQFVY+LDF+DRSFDFVFSRDLDR+S 
Sbjct: 121 VGEESSSAVLALRDMGFSDVMGVGQRPFFSLRRKQFVYKLDFKDRSFDFVFSRDLDRHSA 180

Query: 241 PALLVLEIERVLKPGGIGAVIVGTSGSMPNNLIRAATPVSSLLKTSTVMHVGHVNNLTLV 300
           PALLVLEIERVL+PGGIGAVIVG +GS+PNNLIRAATPVSSLLKTS+VMHV HVNN  LV
Sbjct: 181 PALLVLEIERVLRPGGIGAVIVGATGSLPNNLIRAATPVSSLLKTSSVMHVRHVNNSILV 240

Query: 301 VFKKKPEEFHQFERRLSSECRSLTRNRPLIQKMEPLVKEKPVGFDEKLSYLPKLVAASNG 360
           VFKKK EEF + E R SSECRSLTRN+PLI KMEPLVK KPVGFD+K+SYLPKLV ASN 
Sbjct: 241 VFKKKLEEFGRLEPRFSSECRSLTRNKPLIPKMEPLVKVKPVGFDKKVSYLPKLVDASNW 300

Query: 361 EKLVYVNIGTGKRLNSTDTDWFPPSYPVDRRDFNVYFVDHDMSALATHIHNPGVTFVYHP 420
           EK VYVNIGTGKRLN T+TDWFPPSYP+DRRDFNVYFVDHDMSAL THIH PG+TFVYHP
Sbjct: 301 EKFVYVNIGTGKRLNYTNTDWFPPSYPMDRRDFNVYFVDHDMSALTTHIHKPGITFVYHP 360

Query: 421 GLAGTDQTTDNDGSADDEDEEPYVD-DEFDFLSWFKETVQYADFVVLKMDAGKEELKFLS 480
           GLAGTDQTTDND   ++EDEEPYVD ++FDFLSWFKETVQY+DFVVLKMDAGKEELKFLS
Sbjct: 361 GLAGTDQTTDNDNDTNNEDEEPYVDNNDFDFLSWFKETVQYSDFVVLKMDAGKEELKFLS 420

Query: 481 DLFESGIICWVDEVFLSCRDDGVDEDGELKRDCMDLFNDLRNNGVYVHQWFLDAPST 537
           DLFESG+IC VDE+FLSCR DGVDEDGELKRDCMDLF DLRN+GVYVHQWFLDAPS+
Sbjct: 421 DLFESGVICRVDELFLSCR-DGVDEDGELKRDCMDLFKDLRNSGVYVHQWFLDAPSS 476

BLAST of Spg033528 vs. NCBI nr
Match: XP_023528754.1 (uncharacterized protein LOC111791594 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 804.7 bits (2077), Expect = 5.0e-229
Identity = 402/477 (84.28%), Postives = 439/477 (92.03%), Query Frame = 0

Query: 61  MSLKAVKWQIVHGAMARRVVVRTFIFALAVSTVPLLHILMGADFGVIPSVIFRDCVVMSD 120
           MSLKAVKWQI HGA+ARRVV+RTF+ ALAVSTVPLLHIL G DFG    VIFRDCVV S 
Sbjct: 1   MSLKAVKWQIAHGALARRVVIRTFLLALAVSTVPLLHILTGTDFG----VIFRDCVVKSG 60

Query: 121 DSNVNAKVSRGSYLFRGHFLNPIWVPFVAVHCEEHMNLTTNVVTELIEKKLLNHSAKSLC 180
           D  V A+VSRGSY+F+GHFLNPIW PFVA+HCEE+MNLTTNVV EL+EKKLL+HSAKSLC
Sbjct: 61  D--VEARVSRGSYMFQGHFLNPIWEPFVAIHCEENMNLTTNVVAELMEKKLLDHSAKSLC 120

Query: 181 VGEGSGSAVLALRDMGFSDVMGVGQHRFFSLRRKQFVYELDFEDRSFDFVFSRDLDRYSV 240
           VGEGSGSAVLALRDMGF+DV+GVGQHRFFSLRRK FVYELDF+D+ FDFVFSRDLDRYSV
Sbjct: 121 VGEGSGSAVLALRDMGFNDVIGVGQHRFFSLRRKHFVYELDFKDQYFDFVFSRDLDRYSV 180

Query: 241 PALLVLEIERVLKPGGIGAVIVGTSGSMPNNLIRAATPVSSLLKTSTVMHVGHVNNLTLV 300
           PALLVLEIERV++PGGIGAVIVGT+GS+PNNLIRAA PVSSLLK STVMHVGHVNNLTLV
Sbjct: 181 PALLVLEIERVIRPGGIGAVIVGTTGSVPNNLIRAAIPVSSLLKASTVMHVGHVNNLTLV 240

Query: 301 VFKKKPEEFHQFERRLSSECRSLTRNRPLIQKMEPLVKEKPVGFDEKLSYLPKLVAASNG 360
           VFKKK EEF   E R S ECRSLTRN+PLI KMEPLVK KPVGFD+KL+YLPKLV ASNG
Sbjct: 241 VFKKKLEEFAHLEPRPSFECRSLTRNKPLIPKMEPLVKAKPVGFDKKLAYLPKLVNASNG 300

Query: 361 EKLVYVNIGTGKRLNSTDTDWFPPSYPVDRRDFNVYFVDHDMSALATHIHNPGVTFVYHP 420
           EKLVYVNIGTGKRLN T+ DWFPPSYPVDRRDFNVYFVD+DMS+LA HIH PGVTFVYHP
Sbjct: 301 EKLVYVNIGTGKRLNYTNRDWFPPSYPVDRRDFNVYFVDYDMSSLAAHIHMPGVTFVYHP 360

Query: 421 GLAGTDQTTDNDGSADDEDEEPYVDDEFDFLSWFKETVQYADFVVLKMDAGKEELKFLSD 480
           GLAGTD+TTDNDG+ D+++EEPY+DDEFDFLSWFKETVQ+A+FVVLKMDAGKEELKFLSD
Sbjct: 361 GLAGTDRTTDNDGADDEDEEEPYIDDEFDFLSWFKETVQHANFVVLKMDAGKEELKFLSD 420

Query: 481 LFESGIICWVDEVFLSCRDDGVDEDGEL-KRDCMDLFNDLRNNGVYVHQWFLDAPST 537
           LFES +ICWVDE+FLSCR DGVDEDGE+ KRDCMDLF DLRN+GVYVHQWFLDAPS+
Sbjct: 421 LFESRVICWVDELFLSCR-DGVDEDGEVKKRDCMDLFKDLRNSGVYVHQWFLDAPSS 470

BLAST of Spg033528 vs. NCBI nr
Match: XP_004137970.1 (uncharacterized protein LOC101213900 [Cucumis sativus] >KGN58954.1 hypothetical protein Csa_001707 [Cucumis sativus])

HSP 1 Score: 803.5 bits (2074), Expect = 1.1e-228
Identity = 400/475 (84.21%), Postives = 437/475 (92.00%), Query Frame = 0

Query: 61  MSLKAVKWQIVHGAMARRVVVRTFIFALAVSTVPLLHILMGADFGVIPSVIFRDCVVMSD 120
           M+LKAVKWQI+HGA+ARR+VVR F+ ALAVS VPLLHI MGADFGVIPSVIFRDC V   
Sbjct: 1   MNLKAVKWQIIHGALARRIVVRIFLLALAVSAVPLLHIFMGADFGVIPSVIFRDCAVKYG 60

Query: 121 DSNVNAKVSRGSYLFRGHFLNPIWVPFVAVHCEEHMNLTTNVVTELIEKKLLNHSAKSLC 180
           D  V AKVSRGSY+F+GHFLN IWVPFVA+HCEE+ NLTTNVV EL+EKKLLNH+AKSLC
Sbjct: 61  D--VEAKVSRGSYMFQGHFLNSIWVPFVAMHCEEYKNLTTNVVAELMEKKLLNHTAKSLC 120

Query: 181 VGEGSGSAVLALRDMGFSDVMGVGQHRFFSLRRKQFVYELDFEDRSFDFVFSRDLDRYSV 240
           VGEGSGSAVLALRD+GFSDV+GVGQHRFFSLRRKQFVYELDF+   FDFVFSRDLDRYSV
Sbjct: 121 VGEGSGSAVLALRDIGFSDVIGVGQHRFFSLRRKQFVYELDFKSGYFDFVFSRDLDRYSV 180

Query: 241 PALLVLEIERVLKPGGIGAVIVGTSGSMPNNLIRAATPVSSLLKTSTVMHVGHVNNLTLV 300
           PALLVLEIERVL+PGGIGAVIV TS SMPNNLIRAATPVSSLLKTSTVMHVGHVNNLTLV
Sbjct: 181 PALLVLEIERVLRPGGIGAVIVSTSESMPNNLIRAATPVSSLLKTSTVMHVGHVNNLTLV 240

Query: 301 VFKKKPEEF-HQFERRLSSECRSLTRNRPLIQKMEPLVKEKPVGFDEKLSYLPKLVAASN 360
           VFKKK EE+ H  E RLSSECRSLTRN+PLI K+EPLVKE+PVGFD+KLSYLPK V  S+
Sbjct: 241 VFKKKFEEYRHLEEPRLSSECRSLTRNKPLIPKLEPLVKERPVGFDKKLSYLPKFVDVSS 300

Query: 361 GEKLVYVNIGTGKRLNSTDTDWFPPSYPVDRRDFNVYFVDHDMSALATHIHNPGVTFVYH 420
           G++L+YVNIGTGKRLN T+TDWFPPSYPV RRDFNVYFVDHDMS+LATHIHNPGVTFVYH
Sbjct: 301 GKRLIYVNIGTGKRLNHTNTDWFPPSYPVARRDFNVYFVDHDMSSLATHIHNPGVTFVYH 360

Query: 421 PGLAGTDQTTDNDGSADDEDEEPYVDDEFDFLSWFKETVQYADFVVLKMDAGKEELKFLS 480
           P LAGTDQTTD+D +ADDEDEEPY+DDEFDFLSWFKETVQ++DFVVLKMDAGKEELKFLS
Sbjct: 361 PALAGTDQTTDSDDAADDEDEEPYIDDEFDFLSWFKETVQHSDFVVLKMDAGKEELKFLS 420

Query: 481 DLFESGIICWVDEVFLSCRDDGVDEDGEL-KRDCMDLFNDLRNNGVYVHQWFLDA 534
           DLFESG+ICWVDEVFLSCRD   +EDG+L KR+C DL+ DLRN+GVYVHQWFLDA
Sbjct: 421 DLFESGVICWVDEVFLSCRDGVDEEDGDLKKRECTDLYKDLRNSGVYVHQWFLDA 473

BLAST of Spg033528 vs. ExPASy TrEMBL
Match: A0A5A7TPK3 (Methyltransferase type 11 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002490 PE=4 SV=1)

HSP 1 Score: 806.2 bits (2081), Expect = 8.2e-230
Identity = 398/474 (83.97%), Postives = 436/474 (91.98%), Query Frame = 0

Query: 61  MSLKAVKWQIVHGAMARRVVVRTFIFALAVSTVPLLHILMGADFGVIPSVIFRDCVVMSD 120
           M+LKAVKWQI+HGA+ARR+VVR F+ AL VS VPLLHI MGADFGVIPSVIFRDC V   
Sbjct: 1   MNLKAVKWQIIHGALARRIVVRIFLLALTVSAVPLLHIFMGADFGVIPSVIFRDCGVKYG 60

Query: 121 DSNVNAKVSRGSYLFRGHFLNPIWVPFVAVHCEEHMNLTTNVVTELIEKKLLNHSAKSLC 180
           D  + AKVSRGSY+F+GHFLNPIWVPFVA+HCEE+ NLTTNVV EL+EKKLLNHSAKSLC
Sbjct: 61  D--MEAKVSRGSYMFQGHFLNPIWVPFVAMHCEEYKNLTTNVVAELMEKKLLNHSAKSLC 120

Query: 181 VGEGSGSAVLALRDMGFSDVMGVGQHRFFSLRRKQFVYELDFEDRSFDFVFSRDLDRYSV 240
           VGEGSGSAVLALRD+GFSDV+GV QHRFFSLRRKQFVYELDF+   FDFVFSRDLDR+SV
Sbjct: 121 VGEGSGSAVLALRDIGFSDVIGVDQHRFFSLRRKQFVYELDFKSGYFDFVFSRDLDRHSV 180

Query: 241 PALLVLEIERVLKPGGIGAVIVGTSGSMPNNLIRAATPVSSLLKTSTVMHVGHVNNLTLV 300
           PALLVLEIERVL+PGGIGAVIV +SGSMPNNLIRAATPVSSLLKTSTVMHVGHVNNLTLV
Sbjct: 181 PALLVLEIERVLRPGGIGAVIVSSSGSMPNNLIRAATPVSSLLKTSTVMHVGHVNNLTLV 240

Query: 301 VFKKKPEEFHQFERRLSSECRSLTRNRPLIQKMEPLVKEKPVGFDEKLSYLPKLVAASNG 360
           VFKKK EE    ERR+SSECRSLTRN+PLI+K+EPLVKE PVGFD+KLSYLPK V  S+G
Sbjct: 241 VFKKKLEEHRHLERRMSSECRSLTRNKPLIRKLEPLVKEGPVGFDKKLSYLPKFVDVSSG 300

Query: 361 EKLVYVNIGTGKRLNSTDTDWFPPSYPVDRRDFNVYFVDHDMSALATHIHNPGVTFVYHP 420
           ++L+YVNIGTGKRLN T+TDWFPPSYPVDRRDFNVYFVDHDMS LATHIHNPGVTFVYHP
Sbjct: 301 KRLIYVNIGTGKRLNHTNTDWFPPSYPVDRRDFNVYFVDHDMSYLATHIHNPGVTFVYHP 360

Query: 421 GLAGTDQTTDNDGSADDEDEEPYVDDEFDFLSWFKETVQYADFVVLKMDAGKEELKFLSD 480
            LAG DQTTD+D +ADDEDEEPY+DDEFDFLSWFKETVQ++DFVVLKMDAGKEELKFLSD
Sbjct: 361 ALAGIDQTTDSDDAADDEDEEPYIDDEFDFLSWFKETVQHSDFVVLKMDAGKEELKFLSD 420

Query: 481 LFESGIICWVDEVFLSCRDDGVDEDGEL-KRDCMDLFNDLRNNGVYVHQWFLDA 534
           LFESG+ICWVDEVFLSCRD   +EDG+L KR+CMDL+ DLRN+GVYVHQWFLDA
Sbjct: 421 LFESGVICWVDEVFLSCRDGVDEEDGDLKKRECMDLYKDLRNSGVYVHQWFLDA 472

BLAST of Spg033528 vs. ExPASy TrEMBL
Match: A0A1S4DUH7 (uncharacterized protein LOC107990602 OS=Cucumis melo OX=3656 GN=LOC107990602 PE=4 SV=1)

HSP 1 Score: 806.2 bits (2081), Expect = 8.2e-230
Identity = 398/474 (83.97%), Postives = 436/474 (91.98%), Query Frame = 0

Query: 61  MSLKAVKWQIVHGAMARRVVVRTFIFALAVSTVPLLHILMGADFGVIPSVIFRDCVVMSD 120
           M+LKAVKWQI+HGA+ARR+VVR F+ AL VS VPLLHI MGADFGVIPSVIFRDC V   
Sbjct: 1   MNLKAVKWQIIHGALARRIVVRIFLLALTVSAVPLLHIFMGADFGVIPSVIFRDCGVKYG 60

Query: 121 DSNVNAKVSRGSYLFRGHFLNPIWVPFVAVHCEEHMNLTTNVVTELIEKKLLNHSAKSLC 180
           D  + AKVSRGSY+F+GHFLNPIWVPFVA+HCEE+ NLTTNVV EL+EKKLLNHSAKSLC
Sbjct: 61  D--MEAKVSRGSYMFQGHFLNPIWVPFVAMHCEEYKNLTTNVVAELMEKKLLNHSAKSLC 120

Query: 181 VGEGSGSAVLALRDMGFSDVMGVGQHRFFSLRRKQFVYELDFEDRSFDFVFSRDLDRYSV 240
           VGEGSGSAVLALRD+GFSDV+GV QHRFFSLRRKQFVYELDF+   FDFVFSRDLDR+SV
Sbjct: 121 VGEGSGSAVLALRDIGFSDVIGVDQHRFFSLRRKQFVYELDFKSGYFDFVFSRDLDRHSV 180

Query: 241 PALLVLEIERVLKPGGIGAVIVGTSGSMPNNLIRAATPVSSLLKTSTVMHVGHVNNLTLV 300
           PALLVLEIERVL+PGGIGAVIV +SGSMPNNLIRAATPVSSLLKTSTVMHVGHVNNLTLV
Sbjct: 181 PALLVLEIERVLRPGGIGAVIVSSSGSMPNNLIRAATPVSSLLKTSTVMHVGHVNNLTLV 240

Query: 301 VFKKKPEEFHQFERRLSSECRSLTRNRPLIQKMEPLVKEKPVGFDEKLSYLPKLVAASNG 360
           VFKKK EE    ERR+SSECRSLTRN+PLI+K+EPLVKE PVGFD+KLSYLPK V  S+G
Sbjct: 241 VFKKKLEEHRHLERRMSSECRSLTRNKPLIRKLEPLVKEGPVGFDKKLSYLPKFVDVSSG 300

Query: 361 EKLVYVNIGTGKRLNSTDTDWFPPSYPVDRRDFNVYFVDHDMSALATHIHNPGVTFVYHP 420
           ++L+YVNIGTGKRLN T+TDWFPPSYPVDRRDFNVYFVDHDMS LATHIHNPGVTFVYHP
Sbjct: 301 KRLIYVNIGTGKRLNHTNTDWFPPSYPVDRRDFNVYFVDHDMSYLATHIHNPGVTFVYHP 360

Query: 421 GLAGTDQTTDNDGSADDEDEEPYVDDEFDFLSWFKETVQYADFVVLKMDAGKEELKFLSD 480
            LAG DQTTD+D +ADDEDEEPY+DDEFDFLSWFKETVQ++DFVVLKMDAGKEELKFLSD
Sbjct: 361 ALAGIDQTTDSDDAADDEDEEPYIDDEFDFLSWFKETVQHSDFVVLKMDAGKEELKFLSD 420

Query: 481 LFESGIICWVDEVFLSCRDDGVDEDGEL-KRDCMDLFNDLRNNGVYVHQWFLDA 534
           LFESG+ICWVDEVFLSCRD   +EDG+L KR+CMDL+ DLRN+GVYVHQWFLDA
Sbjct: 421 LFESGVICWVDEVFLSCRDGVDEEDGDLKKRECMDLYKDLRNSGVYVHQWFLDA 472

BLAST of Spg033528 vs. ExPASy TrEMBL
Match: A0A0A0LDG5 (Methyltransf_11 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G739000 PE=4 SV=1)

HSP 1 Score: 803.5 bits (2074), Expect = 5.3e-229
Identity = 400/475 (84.21%), Postives = 437/475 (92.00%), Query Frame = 0

Query: 61  MSLKAVKWQIVHGAMARRVVVRTFIFALAVSTVPLLHILMGADFGVIPSVIFRDCVVMSD 120
           M+LKAVKWQI+HGA+ARR+VVR F+ ALAVS VPLLHI MGADFGVIPSVIFRDC V   
Sbjct: 1   MNLKAVKWQIIHGALARRIVVRIFLLALAVSAVPLLHIFMGADFGVIPSVIFRDCAVKYG 60

Query: 121 DSNVNAKVSRGSYLFRGHFLNPIWVPFVAVHCEEHMNLTTNVVTELIEKKLLNHSAKSLC 180
           D  V AKVSRGSY+F+GHFLN IWVPFVA+HCEE+ NLTTNVV EL+EKKLLNH+AKSLC
Sbjct: 61  D--VEAKVSRGSYMFQGHFLNSIWVPFVAMHCEEYKNLTTNVVAELMEKKLLNHTAKSLC 120

Query: 181 VGEGSGSAVLALRDMGFSDVMGVGQHRFFSLRRKQFVYELDFEDRSFDFVFSRDLDRYSV 240
           VGEGSGSAVLALRD+GFSDV+GVGQHRFFSLRRKQFVYELDF+   FDFVFSRDLDRYSV
Sbjct: 121 VGEGSGSAVLALRDIGFSDVIGVGQHRFFSLRRKQFVYELDFKSGYFDFVFSRDLDRYSV 180

Query: 241 PALLVLEIERVLKPGGIGAVIVGTSGSMPNNLIRAATPVSSLLKTSTVMHVGHVNNLTLV 300
           PALLVLEIERVL+PGGIGAVIV TS SMPNNLIRAATPVSSLLKTSTVMHVGHVNNLTLV
Sbjct: 181 PALLVLEIERVLRPGGIGAVIVSTSESMPNNLIRAATPVSSLLKTSTVMHVGHVNNLTLV 240

Query: 301 VFKKKPEEF-HQFERRLSSECRSLTRNRPLIQKMEPLVKEKPVGFDEKLSYLPKLVAASN 360
           VFKKK EE+ H  E RLSSECRSLTRN+PLI K+EPLVKE+PVGFD+KLSYLPK V  S+
Sbjct: 241 VFKKKFEEYRHLEEPRLSSECRSLTRNKPLIPKLEPLVKERPVGFDKKLSYLPKFVDVSS 300

Query: 361 GEKLVYVNIGTGKRLNSTDTDWFPPSYPVDRRDFNVYFVDHDMSALATHIHNPGVTFVYH 420
           G++L+YVNIGTGKRLN T+TDWFPPSYPV RRDFNVYFVDHDMS+LATHIHNPGVTFVYH
Sbjct: 301 GKRLIYVNIGTGKRLNHTNTDWFPPSYPVARRDFNVYFVDHDMSSLATHIHNPGVTFVYH 360

Query: 421 PGLAGTDQTTDNDGSADDEDEEPYVDDEFDFLSWFKETVQYADFVVLKMDAGKEELKFLS 480
           P LAGTDQTTD+D +ADDEDEEPY+DDEFDFLSWFKETVQ++DFVVLKMDAGKEELKFLS
Sbjct: 361 PALAGTDQTTDSDDAADDEDEEPYIDDEFDFLSWFKETVQHSDFVVLKMDAGKEELKFLS 420

Query: 481 DLFESGIICWVDEVFLSCRDDGVDEDGEL-KRDCMDLFNDLRNNGVYVHQWFLDA 534
           DLFESG+ICWVDEVFLSCRD   +EDG+L KR+C DL+ DLRN+GVYVHQWFLDA
Sbjct: 421 DLFESGVICWVDEVFLSCRDGVDEEDGDLKKRECTDLYKDLRNSGVYVHQWFLDA 473

BLAST of Spg033528 vs. ExPASy TrEMBL
Match: A0A6J1H8W6 (uncharacterized protein LOC111461131 OS=Cucurbita moschata OX=3662 GN=LOC111461131 PE=4 SV=1)

HSP 1 Score: 801.6 bits (2069), Expect = 2.0e-228
Identity = 399/477 (83.65%), Postives = 433/477 (90.78%), Query Frame = 0

Query: 61  MSLKAVKWQIVHGAMARRVVVRTFIFALAVSTVPLLHILMGADFGVIPSVIFRDCVVMSD 120
           MSLK +KWQIVHGA+ARRV+VRTF+FALAVS VPLLHIL GA+FG IP VIFRDC V  D
Sbjct: 1   MSLKGMKWQIVHGALARRVIVRTFLFALAVSAVPLLHILTGANFGEIPPVIFRDCAVKCD 60

Query: 121 DSNVNAKVSRGSYLFRGHFLNPIWVPFVAVHCEEHMNLTTNVVTELIEKKLLNHSAKSLC 180
             N NAK SRGSYLF+GHFLNP+WVPFV +HCEEHMNLTTNVV EL+EKKLLNH  KSLC
Sbjct: 61  YVNSNAKDSRGSYLFQGHFLNPVWVPFVGMHCEEHMNLTTNVVIELMEKKLLNHGVKSLC 120

Query: 181 VGEGSGSAVLALRDMGFSDVMGVGQHRFFSLRRKQFVYELDFEDRSFDFVFSRDLDRYSV 240
           VGE S SAVLALRDMGFSDVMGVGQ  FFSLRRKQFVY+LDF+DRSFDFVFSRDLDR+S 
Sbjct: 121 VGEESSSAVLALRDMGFSDVMGVGQRPFFSLRRKQFVYKLDFKDRSFDFVFSRDLDRHSA 180

Query: 241 PALLVLEIERVLKPGGIGAVIVGTSGSMPNNLIRAATPVSSLLKTSTVMHVGHVNNLTLV 300
           PALLVLEIERVL+PGGIGAVIVG +GS+PNNLIRAATPVSSLLKTS+VMHV HVNN  LV
Sbjct: 181 PALLVLEIERVLRPGGIGAVIVGATGSLPNNLIRAATPVSSLLKTSSVMHVRHVNNSILV 240

Query: 301 VFKKKPEEFHQFERRLSSECRSLTRNRPLIQKMEPLVKEKPVGFDEKLSYLPKLVAASNG 360
           VFKKK EEF + E R SSECRSLTRN+PLI KMEPLVK KPVGFD+K+SYLPKLV ASN 
Sbjct: 241 VFKKKLEEFGRLEPRFSSECRSLTRNKPLIPKMEPLVKVKPVGFDKKVSYLPKLVDASNW 300

Query: 361 EKLVYVNIGTGKRLNSTDTDWFPPSYPVDRRDFNVYFVDHDMSALATHIHNPGVTFVYHP 420
           EK VYVNIGTGKRL+ T+TDWFPPSYP+DRRDFNVYFVDHDMSALATHIH PG+TFVYHP
Sbjct: 301 EKFVYVNIGTGKRLDYTNTDWFPPSYPMDRRDFNVYFVDHDMSALATHIHKPGITFVYHP 360

Query: 421 GLAGTDQTTDNDGSADDEDEEPYVD-DEFDFLSWFKETVQYADFVVLKMDAGKEELKFLS 480
           GLAGTDQTTDND   ++EDEEPYVD ++FDFLSWFKETVQ++DFVVLKMDAGKEELKFLS
Sbjct: 361 GLAGTDQTTDNDNDTNNEDEEPYVDNNDFDFLSWFKETVQHSDFVVLKMDAGKEELKFLS 420

Query: 481 DLFESGIICWVDEVFLSCRDDGVDEDGELKRDCMDLFNDLRNNGVYVHQWFLDAPST 537
           DLFESG+IC VDE+FLSCR DGVDEDGELKRDCMDLF DLRN+GVYVHQWFLDAPS+
Sbjct: 421 DLFESGVICRVDELFLSCR-DGVDEDGELKRDCMDLFKDLRNSGVYVHQWFLDAPSS 476

BLAST of Spg033528 vs. ExPASy TrEMBL
Match: A0A6J1J7J6 (uncharacterized protein LOC111481971 OS=Cucurbita maxima OX=3661 GN=LOC111481971 PE=4 SV=1)

HSP 1 Score: 798.9 bits (2062), Expect = 1.3e-227
Identity = 399/477 (83.65%), Postives = 439/477 (92.03%), Query Frame = 0

Query: 61  MSLKAVKWQIVHGAMARRVVVRTFIFALAVSTVPLLHILMGADFGVIPSVIFRDCVVMSD 120
           MSL+AVKWQI HGA+ARRVV+RTF+ ALAVSTVPL+HIL GADFG    VIFRDCVV S 
Sbjct: 1   MSLQAVKWQIAHGALARRVVIRTFLLALAVSTVPLVHILTGADFG----VIFRDCVVKSG 60

Query: 121 DSNVNAKVSRGSYLFRGHFLNPIWVPFVAVHCEEHMNLTTNVVTELIEKKLLNHSAKSLC 180
           D  V A+VSRGSY+F+GHFLNPIW PFVA+HCEE+MNLTTNVV EL+EKKLL+HSAKSLC
Sbjct: 61  D--VEARVSRGSYMFQGHFLNPIWEPFVAIHCEENMNLTTNVVAELMEKKLLDHSAKSLC 120

Query: 181 VGEGSGSAVLALRDMGFSDVMGVGQHRFFSLRRKQFVYELDFEDRSFDFVFSRDLDRYSV 240
           VGEGSGSAVLALRDMGF+DV+GVGQHRFFSLRRK FVYELDF+D+ FDFVFSRDLDRYSV
Sbjct: 121 VGEGSGSAVLALRDMGFNDVIGVGQHRFFSLRRKHFVYELDFKDQYFDFVFSRDLDRYSV 180

Query: 241 PALLVLEIERVLKPGGIGAVIVGTSGSMPNNLIRAATPVSSLLKTSTVMHVGHVNNLTLV 300
           PALLVLEIERV++PGGIGAVIVGTS S+PNNLIRAA PVSSLLK STVMHVGHVNNLTLV
Sbjct: 181 PALLVLEIERVIRPGGIGAVIVGTSDSVPNNLIRAAIPVSSLLKASTVMHVGHVNNLTLV 240

Query: 301 VFKKKPEEFHQFERRLSSECRSLTRNRPLIQKMEPLVKEKPVGFDEKLSYLPKLVAASNG 360
           VFKKK EEF   E R S +CRSLTRN+PLI KMEPLVK KPVGFD+KL+YLPKLV ASNG
Sbjct: 241 VFKKKLEEFAHLEPRPSFDCRSLTRNKPLIPKMEPLVKAKPVGFDKKLAYLPKLVNASNG 300

Query: 361 EKLVYVNIGTGKRLNSTDTDWFPPSYPVDRRDFNVYFVDHDMSALATHIHNPGVTFVYHP 420
           EKLVYVNIGTGKRLN T+ DWFPPSYPVDRRDFNVYFVD+DMS+LA HIH PGVTFVYHP
Sbjct: 301 EKLVYVNIGTGKRLNYTNIDWFPPSYPVDRRDFNVYFVDYDMSSLAAHIHMPGVTFVYHP 360

Query: 421 GLAGTDQTTDNDGSADDEDEEPYVDDEFDFLSWFKETVQYADFVVLKMDAGKEELKFLSD 480
           GLAGTD+TTDNDG+ D+++EEPY+DDEFDFLSWFKETVQ+A+FVVLKMDAGKEELKFLSD
Sbjct: 361 GLAGTDRTTDNDGADDEDEEEPYMDDEFDFLSWFKETVQHANFVVLKMDAGKEELKFLSD 420

Query: 481 LFESGIICWVDEVFLSCRDDGVDEDGEL-KRDCMDLFNDLRNNGVYVHQWFLDAPST 537
           LFESG+ICWVDE+FLSC  DGVDEDGE+ KRDCMDL+ DLRN+GVYVHQWFLDAPS+
Sbjct: 421 LFESGVICWVDELFLSC-GDGVDEDGEVKKRDCMDLYKDLRNSGVYVHQWFLDAPSS 470

BLAST of Spg033528 vs. TAIR 10
Match: AT3G53400.1 (BEST Arabidopsis thaliana protein match is: conserved peptide upstream open reading frame 47 (TAIR:AT5G03190.1); Has 285 Blast hits to 285 proteins in 23 species: Archae - 0; Bacteria - 0; Metazoa - 1; Fungi - 0; Plants - 279; Viruses - 0; Other Eukaryotes - 5 (source: NCBI BLink). )

HSP 1 Score: 364.8 bits (935), Expect = 1.2e-100
Identity = 209/483 (43.27%), Postives = 290/483 (60.04%), Query Frame = 0

Query: 61  MSLKAVKWQIVHGAMARRVVVRTFIFALAVSTVPLLHILMGADFGVIPSVIFRDCVVMSD 120
           M  + +K +++  +  RRV++R  +   A S V +L  L GA  G     I        D
Sbjct: 1   MGFEVLKLKLMPSSFRRRVILRALVIVCAFSVVSVLRNLAGAYEG---ETINNTQPCKVD 60

Query: 121 DSNVNAK------VSRGSYLFRGHFLNPIWVPFVAVHCEEHMNLTTNVVTELIEKKLLNH 180
           +  VN         S    LF   FL P+W    +  C++++ LTT VV EL    LL++
Sbjct: 61  ECAVNFAFLGPFLFSGNGLLFSNRFLKPVWNYLESDKCKDNIYLTTQVVRELTGMNLLSN 120

Query: 181 SAKSLCVGEGSGSAVLALRDMGFSDVMGVGQHRFFSLRRKQFVYELDFEDRSFDFVFSRD 240
            +K+LC+G  S SAVLA+   G SDV        F+ + ++F  EL +ED SF FVFS D
Sbjct: 121 DSKALCIGRRSVSAVLAMNRQGISDVSVAYMPPVFAFKHRKFTSELHYEDASFGFVFSMD 180

Query: 241 LDRYSVPALLVLEIERVLKPGGIGAVIVG-TSGSMPNNLIRAATPVSSLLKTSTVMHVGH 300
           L+  +VPA LV EIER+LKPGG GA++VG TSGS  N L+R+ +PVSSLLK S+V+HV  
Sbjct: 181 LESVAVPASLVYEIERILKPGGTGAMLVGTTSGSDSNELVRSVSPVSSLLKNSSVVHVAS 240

Query: 301 VNNLTLVVFKKKPEE---FHQFERRLSSECRSLTRNRPLIQKMEPLVKEKPVGFDEKLSY 360
           +    LVVFK+  E+     Q      ++C S+  NRP I  +EPL+ EK   F+ ++ Y
Sbjct: 241 LGKQVLVVFKRDGEDSFRLDQTHHDFPADCSSVLNNRPYIGLLEPLLDEKRSDFERRIHY 300

Query: 361 LPKLVAASNGEKLVYVNIGTGKRLNSTDTDWFPPSYPVDRRDFNVYFVDHDMSALATHIH 420
           LP+ +  S+ ++LVY++IG    + +  ++WF PSYP+DR+ FN YFV H+ S L +++ 
Sbjct: 301 LPEFIDLSSRKRLVYIDIGAVDHVKAR-SNWFFPSYPIDRKAFNSYFVHHNTSILTSYVK 360

Query: 421 NPGVTFVYHPGLAGTDQTTDNDGSADDEDEEPYV-DDEFDFLSWFKETVQYADFVVLKMD 480
           +PGVTF+YHPGLA T  T  N G    + EEP+V DD FDFL+WFKET  +ADFVVLKM+
Sbjct: 361 SPGVTFIYHPGLAATKTTIANTG----DHEEPFVEDDSFDFLAWFKETASFADFVVLKMN 420

Query: 481 AGKEELKFLSDLFESGIICWVDEVFLSCRDDGVDEDGELKRDCMDLFNDLRNNGVYVHQW 533
               ELKFLS+L ++G IC VDE+FL C             DC  +   LRN+GV+VHQW
Sbjct: 421 TSDTELKFLSELIKTGAICSVDELFLHCTG---------YSDCTGIIKSLRNSGVFVHQW 466

BLAST of Spg033528 vs. TAIR 10
Match: AT5G03190.2 (conserved peptide upstream open reading frame 47 )

HSP 1 Score: 334.7 bits (857), Expect = 1.4e-91
Identity = 211/538 (39.22%), Postives = 309/538 (57.43%), Query Frame = 0

Query: 1   MGTWDGRIRRMRVPKRVWLILQKSMGLLVGGNHTSSSIPVLFVFDDDKRTLSGLGLAIGE 60
           MGT    ++ +RVP+RVW   Q S+ LLVGGNHTSS                        
Sbjct: 8   MGT---EVKSIRVPRRVWFGSQISIVLLVGGNHTSS------------------------ 67

Query: 61  MSLKAVKWQIVHGAMARRVVVRTFIFALAVSTVPLLHILMGADFGVIPSVIFRDCVVMSD 120
                           R  + R  I A A+S VPLL +          + +F D   + D
Sbjct: 68  ----------------RHALFRALIIASALSVVPLLEL-------TAKNHVFGDHGAV-D 127

Query: 121 DSNVNAKVSRGSYLFRGHFLNPIWVPFVAVHCEEHMNLT-TNVVTELIEKKLLNHSAKSL 180
             ++   V  G  LF    + P W     +  E++  +   ++V EL+  KLL++ AK L
Sbjct: 128 LVDLGRFVIPGPKLFFHKLVQPFW---GKIETEKYPQVVIADLVDELMGLKLLHYDAKIL 187

Query: 181 CVGEGSGSAVLALRDMGFSDVMGVGQHRFFSLRRKQFVYELDFE-DRSFDFVFSRDLDRY 240
           C+G+GS SAV   ++MGFS V GV +H  FS   ++ V EL+   D+SFDFV   D+D  
Sbjct: 188 CIGQGSDSAVSGFKEMGFSVVQGVPKHPLFSFFSRKHVNELELSGDKSFDFVLCGDVDHV 247

Query: 241 SVPALLVLEIERVLKPGGIGAVIVGTSGSMPNNLIRAATPVSSLLKTSTVMHVGHVNNLT 300
           + PALLVLE+ERVLKPGG GAV+V T+    N L+++   V+S LK S ++ V +++  T
Sbjct: 248 ASPALLVLEMERVLKPGGTGAVLVSTNA---NRLVKS---VTSGLKQSEIVRVNNLDKFT 307

Query: 301 LVVFKKKPEE--FHQFERRLSSECRSLTRNRPLIQKMEPLVKEKPVGFDEKLSYLPKLVA 360
           ++VFK+   E  +   + +L  +C+S+  NRP  + MEPL+++KP  F + ++YLPK + 
Sbjct: 308 VIVFKRNVTETAYCIGKSQLPRDCKSVDTNRPYTEFMEPLLEQKPADFPKSVAYLPKFLD 367

Query: 361 ASNGEKLVYVNIGTGKRLNSTDT-DWFPPSYPVDRRDFNVYFVDHDMSALATHIHNPGVT 420
            S  + LVY++IG  + +++  T +WF P YP+D + FNVYFVDH+ S + +++  PGVT
Sbjct: 368 LSLKKSLVYIDIGAAEHMDANLTPNWFFPLYPLDSKAFNVYFVDHNTSVMLSYVKKPGVT 427

Query: 421 FVYHPGLAGTDQTTDNDGSADDEDEEPYVDDE-FDFLSWFKETVQYADFVVLKMDAGKEE 480
           FVYHP LA  + T         E  EP+ +DE FDFL+WF+ET +YADFVVLKM+  + E
Sbjct: 428 FVYHPDLAENNST--GKKITPLEQLEPFPEDERFDFLAWFEETAKYADFVVLKMNTNQVE 475

Query: 481 LKFLSDLFESGIICWVDEVFLSCRDDGVDEDGELKRDCMDLFNDLRNNGVYVHQWFLD 533
           +KFL+ L E+G+IC+VDE+FL C +         K DC+++   LR  GV+VHQW+ D
Sbjct: 488 MKFLTVLLETGVICYVDELFLRCSNH--------KSDCINMLQTLRARGVFVHQWWED 475

BLAST of Spg033528 vs. TAIR 10
Match: AT5G03190.1 (conserved peptide upstream open reading frame 47 )

HSP 1 Score: 325.1 bits (832), Expect = 1.1e-88
Identity = 195/478 (40.79%), Postives = 291/478 (60.88%), Query Frame = 0

Query: 61  MSLKAVKWQIVHGAMARRVVVRTFIFALAVSTVPLLHILMGADFGVIPSVIFRDCVVMSD 120
           M +K +K  I  G+  R  + R  I A A+S VPLL +          + +F D   + D
Sbjct: 1   MGIKILKLNIFRGSTNRHALFRALIIASALSVVPLLEL-------TAKNHVFGDHGAV-D 60

Query: 121 DSNVNAKVSRGSYLFRGHFLNPIWVPFVAVHCEEHMNLT-TNVVTELIEKKLLNHSAKSL 180
             ++   V  G  LF    + P W     +  E++  +   ++V EL+  KLL++ AK L
Sbjct: 61  LVDLGRFVIPGPKLFFHKLVQPFW---GKIETEKYPQVVIADLVDELMGLKLLHYDAKIL 120

Query: 181 CVGEGSGSAVLALRDMGFSDVMGVGQHRFFSLRRKQFVYELDFE-DRSFDFVFSRDLDRY 240
           C+G+GS SAV   ++MGFS V GV +H  FS   ++ V EL+   D+SFDFV   D+D  
Sbjct: 121 CIGQGSDSAVSGFKEMGFSVVQGVPKHPLFSFFSRKHVNELELSGDKSFDFVLCGDVDHV 180

Query: 241 SVPALLVLEIERVLKPGGIGAVIVGTSGSMPNNLIRAATPVSSLLKTSTVMHVGHVNNLT 300
           + PALLVLE+ERVLKPGG GAV+V T+    N L+++   V+S LK S ++ V +++  T
Sbjct: 181 ASPALLVLEMERVLKPGGTGAVLVSTNA---NRLVKS---VTSGLKQSEIVRVNNLDKFT 240

Query: 301 LVVFKKKPEE--FHQFERRLSSECRSLTRNRPLIQKMEPLVKEKPVGFDEKLSYLPKLVA 360
           ++VFK+   E  +   + +L  +C+S+  NRP  + MEPL+++KP  F + ++YLPK + 
Sbjct: 241 VIVFKRNVTETAYCIGKSQLPRDCKSVDTNRPYTEFMEPLLEQKPADFPKSVAYLPKFLD 300

Query: 361 ASNGEKLVYVNIGTGKRLNSTDT-DWFPPSYPVDRRDFNVYFVDHDMSALATHIHNPGVT 420
            S  + LVY++IG  + +++  T +WF P YP+D + FNVYFVDH+ S + +++  PGVT
Sbjct: 301 LSLKKSLVYIDIGAAEHMDANLTPNWFFPLYPLDSKAFNVYFVDHNTSVMLSYVKKPGVT 360

Query: 421 FVYHPGLAGTDQTTDNDGSADDEDEEPYVDDE-FDFLSWFKETVQYADFVVLKMDAGKEE 480
           FVYHP LA  + T         E  EP+ +DE FDFL+WF+ET +YADFVVLKM+  + E
Sbjct: 361 FVYHPDLAENNST--GKKITPLEQLEPFPEDERFDFLAWFEETAKYADFVVLKMNTNQVE 420

Query: 481 LKFLSDLFESGIICWVDEVFLSCRDDGVDEDGELKRDCMDLFNDLRNNGVYVHQWFLD 533
           +KFL+ L E+G+IC+VDE+FL C +         K DC+++   LR  GV+VHQW+ D
Sbjct: 421 MKFLTVLLETGVICYVDELFLRCSNH--------KSDCINMLQTLRARGVFVHQWWED 451

BLAST of Spg033528 vs. TAIR 10
Match: AT5G01710.1 (methyltransferases )

HSP 1 Score: 122.5 bits (306), Expect = 1.1e-27
Identity = 109/421 (25.89%), Postives = 178/421 (42.28%), Query Frame = 0

Query: 160 TNVVTELIEKKLLNHSAKSLCVGEGSGSAVLALRDMGFSDVMGVGQHRFFSLRRKQFVYE 219
           +++  +LI    L+  +K+LCV    G  V +LR++G  + +G+ +     L  +   + 
Sbjct: 95  SSIFQDLIADGYLSPESKTLCVETAIGQEVHSLREIGVKNSVGISKKASRPLVVRGEGHA 154

Query: 220 LDFEDRSFDFVFSRD--LDRYSVPALLVLEIERVLKPGGIGAVIVGTSGSMPNNLIRAAT 279
           + FED +FDFVFS    L +         EI R LKP G   V VG + +   N      
Sbjct: 155 IPFEDNAFDFVFSGGDRLGKSLKQLEFADEITRTLKPEGFAVVHVGATDTYSFNSFLDLF 214

Query: 280 PVSSLLKTSTV----MHVGHVNNLTLVVFKKKPEEFHQFERRLSS--ECRSLTRNRPLIQ 339
               L+K   +      + H+    +  + +     H       S  +C      R LI+
Sbjct: 215 NSCRLVKMRDIDGFDPSMPHIREFVIQKYSEIDGGGHHHRGGGDSGGKCWIPGYKRDLIR 274

Query: 340 KMEPLVKEKPV-------GFDEKLSYLPKLVAASNGEKLVYVNIGTGKRLNSTDTDWFPP 399
             EPL++E+P+          + + Y+P +V      + VYV++G  +   S+   WF  
Sbjct: 275 DAEPLIQEEPLKPWITLKRNIKNIKYVPSMVDIRFKSRYVYVDVG-ARSYGSSIGSWFKK 334

Query: 400 SYPVDRRDFNVYFVDHD--------------MSALATHIHNPGVTFV--YHPGL------ 459
            YP   + F+V+ ++ D              +   A  + N  ++F   + PG       
Sbjct: 335 EYPKQNKTFDVFAIEADKAFHEEYKIKKKVNLLPYAAWVRNETLSFEINHDPGKEVEAKA 394

Query: 460 ----AGTDQTTDNDGSADDEDEEPYVDDEFDFLSWFKETVQYADFVVLKMDAGKEELKFL 519
                G  Q      S+D   E   +   FDF  W K++V+  DFVV+KMD    E   +
Sbjct: 395 KGRGMGRIQPVKKSDSSDLAGEVNLIQG-FDFADWLKKSVRERDFVVMKMDVEGTEFDLI 454

Query: 520 SDLFESGIICWVDEVFLSCR---------DDGVDEDGELKRDCMDLFNDLRNNGVYVHQW 531
             L ++G IC +DE+FL C               +  +    C++LFN LR  GV VHQW
Sbjct: 455 PRLIKTGAICLIDELFLECHYNRWQRCCPGQRSQKYNKTYNQCLELFNSLRQRGVLVHQW 513

BLAST of Spg033528 vs. TAIR 10
Match: AT1G24480.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 63.9 bits (154), Expect = 4.5e-10
Identity = 49/147 (33.33%), Postives = 74/147 (50.34%), Query Frame = 0

Query: 165 ELIEKKLLNHSAKSLCVGEGSGSAVLALRDMGFSDVMGVGQHRFFSLRRKQFVYELDFED 224
           +L  + LL+  +K LC+G   G  V AL+ +G +D +G+    +  L  K   +   F+D
Sbjct: 84  DLKRQGLLSKDSKCLCLGARVGQEVEALKRVGVNDSVGMDLVPYPPLVVKGDFHHQPFDD 143

Query: 225 RSFDFVFSRDLDRYSVPALLVLEIERVLKPGGIGAVIVG---TSGSMPNNLIRAATPVSS 284
            +FDF FS   D    P   V EIER L+PGG+  + V     S     N + +   +  
Sbjct: 144 ETFDFEFSNVFDHALYPDKFVGEIERTLRPGGLCVLHVALSTRSDKYSANDLFSVEALVK 203

Query: 285 LLKTSTVMHVGHVNNL---TLVVFKKK 306
           L + S V+HV +V+     T VVF+KK
Sbjct: 204 LFRQSEVVHVRNVDGFGLDTEVVFRKK 230

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038904424.13.7e-23284.58uncharacterized protein LOC120090789 [Benincasa hispida][more]
XP_016899642.11.7e-22983.97PREDICTED: uncharacterized protein LOC107990602 [Cucumis melo] >KAA0044016.1 Met... [more]
KAG6596469.12.2e-22984.07hypothetical protein SDJN03_09649, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023528754.15.0e-22984.28uncharacterized protein LOC111791594 [Cucurbita pepo subsp. pepo][more]
XP_004137970.11.1e-22884.21uncharacterized protein LOC101213900 [Cucumis sativus] >KGN58954.1 hypothetical ... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5A7TPK38.2e-23083.97Methyltransferase type 11 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A1S4DUH78.2e-23083.97uncharacterized protein LOC107990602 OS=Cucumis melo OX=3656 GN=LOC107990602 PE=... [more]
A0A0A0LDG55.3e-22984.21Methyltransf_11 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G73... [more]
A0A6J1H8W62.0e-22883.65uncharacterized protein LOC111461131 OS=Cucurbita moschata OX=3662 GN=LOC1114611... [more]
A0A6J1J7J61.3e-22783.65uncharacterized protein LOC111481971 OS=Cucurbita maxima OX=3661 GN=LOC111481971... [more]
Match NameE-valueIdentityDescription
AT3G53400.11.2e-10043.27BEST Arabidopsis thaliana protein match is: conserved peptide upstream open read... [more]
AT5G03190.21.4e-9139.22conserved peptide upstream open reading frame 47 [more]
AT5G03190.11.1e-8840.79conserved peptide upstream open reading frame 47 [more]
AT5G01710.11.1e-2725.89methyltransferases [more]
AT1G24480.14.5e-1033.33S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 151..366
e-value: 1.9E-6
score: 29.4
NoneNo IPR availablePANTHERPTHR47291:SF1PEPTIDE UPSTREAM PROTEINcoord: 61..530
NoneNo IPR availablePANTHERPTHR47291PEPTIDE UPSTREAM PROTEINcoord: 61..530
IPR013216Methyltransferase type 11PFAMPF08241Methyltransf_11coord: 181..257
e-value: 5.3E-6
score: 27.0
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 164..269

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg033528.1Spg033528.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008168 methyltransferase activity