Spg031003 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg031003
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionReverse transcriptase
Locationscaffold10: 21766631 .. 21768461 (-)
RNA-Seq ExpressionSpg031003
SyntenySpg031003
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAAACAGAGCACAGGATTTTCGAGCAAAGCAGGAGGATCTCTGGTTTTCTCTGATGTTTTGAGCATTCTGGGGTGTACAAGTTGAATCCTAAGCTCTATTTGGTATGGTTGGAAATATTATTCAATTATCTACAACTTTCAGGAAGAATTCGTGAGCCAAAAATGACTTGAATTAGGTTTAATTTCAGGAGGAAGATAAAGGGTAACAGAGAAGCTCGAATCTGTGTTGTCTGATTTCGGGGCATTTCTGGACAAATCGGTTGGGAATCTCATTTTCATTTCTTCAGGTAAGTTGTAGAGTGTTTGAAAATGAAGAGTTGGATGTGTATTTCATTAATTTTGGTTAAGTATTGAGGAAGTTATGGGTGTTGGAAGCTTGGGTATAGAATCTGGAAAATCTGGAAAGTAGAATGTGAATGTGATTCTTAATCCAATTGTTTAAAAATTCATGAGCATGGAAAGACAAAGCTATCTATGGTTCTAATGCTCGGTTTTGGTTATTTGACAGGCCAAGAGGAAATAGGTCGAGTCTACAAACCGGGGAACTAGATAAGACAGTGAGTGACAAAATGAATTTCGAAAATGTTCTTAAACGGTTTGTTCATGATTATATGTTTTACATGCTTTCCTATGAATGATACATGGTTTTTATGAACGGTTTCAAGCATGAGTTTTGAGCTCAGATTTTCTAATGAGGTTTATATACGAGCACGTGTCTAATGACTTTATGAAAAGTAGCTGAAACGATATCTTTTAAAGCAAAGTATGAATTAGCAAGTACTTCTAAATGATTTTCGGTTTTCCACGAGCAAGCTGAGTAAGCTTAAGTTTTATGAAAGATAACGATAAGCAGGCATGTTTTAATGTTTTCATGTGATTTTCTGAGATTTCTATTGACTACATGACTGAGATATTGAGCCTGAGGCTATATGGTACCGTGTGCACACAGGTTATATTCCGTTGTCGACGTTGAGTGTACTCCGTGACAACGATGCTGTCGTGAGTGCTAGGCGGGCCCCACTACGACAAAGACGATGGGAGTGCTGGGCGGGCCCCACTACATCGTAGGACTAGTAAACGTTGGTTGTACTGGGCGTGCCCTACACAACGTAGATCGGTCATGTTAGTTAAAACGTTTGATATACTTGATATGCCTTGCTAGATTCTTAATGACGGACTTGCGAAATGTTTTAGAAAGCCTTTATCCTTACACGTTTATGTTAAACGCTTTGACTGAGCATATTTATATGTTTAAAGGTTTATCACGTGTTTTGACTTCCAGATTTAGAGTTTTAAAATTAGTCACTCACTGGGCTTCGTAGCTCATTCTTTCAAAATGTTTTCCACTTTTCAGGTAGAGATTGAGCTCCCGGTGCCTGATACACTGCCAACGTCTGCTGAAAGTTCTAGAACAAGCTCCAGTACGTGGGTGGAGTTGTATATTGAGTCTGTTCATATTGTAAATGTTTTGTAAGGATTGGATAATATAGTCCTAGAGGTCGTGGCTGTATAAACCCTAGTTGTATATGTTTTTGGTTGTGATATCGATACCAGATTATTTTCCAAACCGAGTTATGTCAGGGCGGCACCTCGTGTATGTTAAAAGTTTTTTTTCAGGGATTCCGCTGTGTTCTGTTTCATGTAGAATGGCTTATATCTATGATTAGTATGTTTATCAGGTTCAATGTTTCAGTAGGGTCAACAGGGATCGTTAGAGGTGACGATGTCTGTTGGCTTCACGCTGTCTTTCGGGCTAAGCTAGCAGGTGGTCCGGGAGGGGGTGTGACAGTTAGGAACTCAAAGTTCGGCCCAATGAGAGTTCTTTAG

mRNA sequence

ATGGAAAACAGAGCACAGGATTTTCGAGCAAAGCAGGAGGATCTCTGGCCAAGAGGAAATAGGTCGAGTCTACAAACCGGGGAACTAGATAAGACACCTGAGGCTATATGGTACCGTGTGCACACAGGTTATATTCCGTTGTCGACGTTGAGTGTACTCCGTGACAACGATGCTGTCGTAGAGATTGAGCTCCCGGTGCCTGATACACTGCCAACGTCTGCTGAAAGTTCTAGAACAAGCTCCAGTACGTGGGTGGAGTTGTATATTGAGTCTGTTCATATTGTAAATGTTTTGTTCAATGTTTCAGTAGGGTCAACAGGGATCGTTAGAGGTGACGATGTCTGTTGGCTTCACGCTGTCTTTCGGGCTAAGCTAGCAGGTGGTCCGGGAGGGGGTGTGACAGTTAGGAACTCAAAGTTCGGCCCAATGAGAGTTCTTTAG

Coding sequence (CDS)

ATGGAAAACAGAGCACAGGATTTTCGAGCAAAGCAGGAGGATCTCTGGCCAAGAGGAAATAGGTCGAGTCTACAAACCGGGGAACTAGATAAGACACCTGAGGCTATATGGTACCGTGTGCACACAGGTTATATTCCGTTGTCGACGTTGAGTGTACTCCGTGACAACGATGCTGTCGTAGAGATTGAGCTCCCGGTGCCTGATACACTGCCAACGTCTGCTGAAAGTTCTAGAACAAGCTCCAGTACGTGGGTGGAGTTGTATATTGAGTCTGTTCATATTGTAAATGTTTTGTTCAATGTTTCAGTAGGGTCAACAGGGATCGTTAGAGGTGACGATGTCTGTTGGCTTCACGCTGTCTTTCGGGCTAAGCTAGCAGGTGGTCCGGGAGGGGGTGTGACAGTTAGGAACTCAAAGTTCGGCCCAATGAGAGTTCTTTAG

Protein sequence

MENRAQDFRAKQEDLWPRGNRSSLQTGELDKTPEAIWYRVHTGYIPLSTLSVLRDNDAVVEIELPVPDTLPTSAESSRTSSSTWVELYIESVHIVNVLFNVSVGSTGIVRGDDVCWLHAVFRAKLAGGPGGGVTVRNSKFGPMRVL
Homology
BLAST of Spg031003 vs. NCBI nr
Match: KAA0062409.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 167.2 bits (422), Expect = 1.1e-37
Identity = 90/138 (65.22%), Postives = 101/138 (73.19%), Query Frame = 0

Query: 14  DLWPRGNRSSLQTGELDKTPEAIWYRVHTGYIPLSTLSVLRDNDAVVEIELPVPDTLPTS 73
           D+  RGNRSSLQ+GELD T             PLSTLSVLRDNDAVVEIELPVPDTLPTS
Sbjct: 58  DVMLRGNRSSLQSGELDTTV----------IFPLSTLSVLRDNDAVVEIELPVPDTLPTS 117

Query: 74  AESSRTSSSTWVELYIESVHI-------------VNVLFNVSVGSTGIVRGDDVCWLHAV 133
           AESS ++SSTW+ELY ESVH+             ++++F+V VGSTGIVRGDDVCWLHAV
Sbjct: 118 AESSESNSSTWLELYFESVHVEMFCYFTNQVMSRLHLVFSVLVGSTGIVRGDDVCWLHAV 177

Query: 134 FRAKLAGGPGGGVTVRNS 139
           FRAK  GGPGGGVT   S
Sbjct: 178 FRAKTVGGPGGGVTTLES 185

BLAST of Spg031003 vs. NCBI nr
Match: TYK26572.1 (putative Retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 167.2 bits (422), Expect = 1.1e-37
Identity = 90/138 (65.22%), Postives = 101/138 (73.19%), Query Frame = 0

Query: 14  DLWPRGNRSSLQTGELDKTPEAIWYRVHTGYIPLSTLSVLRDNDAVVEIELPVPDTLPTS 73
           D+  RGNRSSLQ+GELD T             PLSTLSVLRDNDAVVEIELPVPDTLPTS
Sbjct: 58  DVMLRGNRSSLQSGELDTTV----------IFPLSTLSVLRDNDAVVEIELPVPDTLPTS 117

Query: 74  AESSRTSSSTWVELYIESVHI-------------VNVLFNVSVGSTGIVRGDDVCWLHAV 133
           AESS ++SSTW+ELY ESVH+             ++++F+V VGSTGIVRGDDVCWLHAV
Sbjct: 118 AESSESNSSTWLELYFESVHVEMFCYFTNQVMSRLHLVFSVLVGSTGIVRGDDVCWLHAV 177

Query: 134 FRAKLAGGPGGGVTVRNS 139
           FRAK  GGPGGGVT   S
Sbjct: 178 FRAKTVGGPGGGVTTLES 185

BLAST of Spg031003 vs. NCBI nr
Match: TYK25935.1 (gag/pol protein [Cucumis melo var. makuwa])

HSP 1 Score: 162.5 bits (410), Expect = 2.7e-36
Identity = 86/133 (64.66%), Postives = 94/133 (70.68%), Query Frame = 0

Query: 16  WPRGNRSSLQTGELDKTPEAIWYRVHTGYIPLSTLSVLRDNDAVVEIELPVPDTLPTSAE 75
           WPRGNR  LQTGELDK              PLSTLSVLRDNDAVVEIELP+PDTLPTSAE
Sbjct: 302 WPRGNRPGLQTGELDKIV----------IFPLSTLSVLRDNDAVVEIELPLPDTLPTSAE 361

Query: 76  SSRTSSSTWVELYIESVHIVNVLFNVS----------VGSTGIVRGDDVCWLHAVFRAKL 135
           SS ++SSTW+ELY ESVH+  + +  S           GS GIVRGDDVCWLHA+FRAK 
Sbjct: 362 SSGSNSSTWLELYFESVHVEMLFYKPSDARAASYVCWEGSAGIVRGDDVCWLHAIFRAKT 421

Query: 136 AGGPGGGVTVRNS 139
           AGGPGGGVT   S
Sbjct: 422 AGGPGGGVTPSKS 424

BLAST of Spg031003 vs. NCBI nr
Match: KAA0040194.1 (gag/pol protein [Cucumis melo var. makuwa])

HSP 1 Score: 162.5 bits (410), Expect = 2.7e-36
Identity = 86/133 (64.66%), Postives = 94/133 (70.68%), Query Frame = 0

Query: 16  WPRGNRSSLQTGELDKTPEAIWYRVHTGYIPLSTLSVLRDNDAVVEIELPVPDTLPTSAE 75
           WPRGNR  LQTGELDK              PLSTLSVLRDNDAVVEIELP+PDTLPTSAE
Sbjct: 302 WPRGNRPGLQTGELDKIV----------IFPLSTLSVLRDNDAVVEIELPLPDTLPTSAE 361

Query: 76  SSRTSSSTWVELYIESVHIVNVLFNVS----------VGSTGIVRGDDVCWLHAVFRAKL 135
           SS ++SSTW+ELY ESVH+  + +  S           GS GIVRGDDVCWLHA+FRAK 
Sbjct: 362 SSGSNSSTWLELYFESVHVEMLFYKPSDARAASYVCWEGSAGIVRGDDVCWLHAIFRAKT 421

Query: 136 AGGPGGGVTVRNS 139
           AGGPGGGVT   S
Sbjct: 422 AGGPGGGVTPSKS 424

BLAST of Spg031003 vs. NCBI nr
Match: TYK11835.1 (uncharacterized protein E5676_scaffold152G00520 [Cucumis melo var. makuwa])

HSP 1 Score: 160.6 bits (405), Expect = 1.0e-35
Identity = 90/137 (65.69%), Postives = 99/137 (72.26%), Query Frame = 0

Query: 15  LWPRGNRSSLQTGELDKTPEAIWYRVHTGYIPLSTLSVLRDNDAVVEIELPVPDTLPTSA 74
           L PRGNR SLQTGELDKT              LSTLSVLRDN+AV  IELPVPDTLPTSA
Sbjct: 61  LGPRGNRLSLQTGELDKTV----------IFSLSTLSVLRDNNAV--IELPVPDTLPTSA 120

Query: 75  ESSRTSSSTWVELYIESVHI-------------VNVLFNVSVGSTGIVRGDDVCWLHAVF 134
           ESS ++SSTW+ELY ESVH+             ++++FNVSVGSTGIVRGDDVCWLHAVF
Sbjct: 121 ESSGSNSSTWLELYFESVHVEMFCSFTNQVMSELHLVFNVSVGSTGIVRGDDVCWLHAVF 180

Query: 135 RAKLAGGPGGGVTVRNS 139
           RAK  GGPGGGVT   S
Sbjct: 181 RAKTVGGPGGGVTTWES 185

BLAST of Spg031003 vs. ExPASy TrEMBL
Match: A0A5A7V9N3 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold154G002110 PE=4 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 5.2e-38
Identity = 90/138 (65.22%), Postives = 101/138 (73.19%), Query Frame = 0

Query: 14  DLWPRGNRSSLQTGELDKTPEAIWYRVHTGYIPLSTLSVLRDNDAVVEIELPVPDTLPTS 73
           D+  RGNRSSLQ+GELD T             PLSTLSVLRDNDAVVEIELPVPDTLPTS
Sbjct: 58  DVMLRGNRSSLQSGELDTTV----------IFPLSTLSVLRDNDAVVEIELPVPDTLPTS 117

Query: 74  AESSRTSSSTWVELYIESVHI-------------VNVLFNVSVGSTGIVRGDDVCWLHAV 133
           AESS ++SSTW+ELY ESVH+             ++++F+V VGSTGIVRGDDVCWLHAV
Sbjct: 118 AESSESNSSTWLELYFESVHVEMFCYFTNQVMSRLHLVFSVLVGSTGIVRGDDVCWLHAV 177

Query: 134 FRAKLAGGPGGGVTVRNS 139
           FRAK  GGPGGGVT   S
Sbjct: 178 FRAKTVGGPGGGVTTLES 185

BLAST of Spg031003 vs. ExPASy TrEMBL
Match: A0A5D3DSY7 (Putative Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold313G001810 PE=4 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 5.2e-38
Identity = 90/138 (65.22%), Postives = 101/138 (73.19%), Query Frame = 0

Query: 14  DLWPRGNRSSLQTGELDKTPEAIWYRVHTGYIPLSTLSVLRDNDAVVEIELPVPDTLPTS 73
           D+  RGNRSSLQ+GELD T             PLSTLSVLRDNDAVVEIELPVPDTLPTS
Sbjct: 58  DVMLRGNRSSLQSGELDTTV----------IFPLSTLSVLRDNDAVVEIELPVPDTLPTS 117

Query: 74  AESSRTSSSTWVELYIESVHI-------------VNVLFNVSVGSTGIVRGDDVCWLHAV 133
           AESS ++SSTW+ELY ESVH+             ++++F+V VGSTGIVRGDDVCWLHAV
Sbjct: 118 AESSESNSSTWLELYFESVHVEMFCYFTNQVMSRLHLVFSVLVGSTGIVRGDDVCWLHAV 177

Query: 134 FRAKLAGGPGGGVTVRNS 139
           FRAK  GGPGGGVT   S
Sbjct: 178 FRAKTVGGPGGGVTTLES 185

BLAST of Spg031003 vs. ExPASy TrEMBL
Match: A0A5A7T9P7 (Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1164G00060 PE=4 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 1.3e-36
Identity = 86/133 (64.66%), Postives = 94/133 (70.68%), Query Frame = 0

Query: 16  WPRGNRSSLQTGELDKTPEAIWYRVHTGYIPLSTLSVLRDNDAVVEIELPVPDTLPTSAE 75
           WPRGNR  LQTGELDK              PLSTLSVLRDNDAVVEIELP+PDTLPTSAE
Sbjct: 302 WPRGNRPGLQTGELDKIV----------IFPLSTLSVLRDNDAVVEIELPLPDTLPTSAE 361

Query: 76  SSRTSSSTWVELYIESVHIVNVLFNVS----------VGSTGIVRGDDVCWLHAVFRAKL 135
           SS ++SSTW+ELY ESVH+  + +  S           GS GIVRGDDVCWLHA+FRAK 
Sbjct: 362 SSGSNSSTWLELYFESVHVEMLFYKPSDARAASYVCWEGSAGIVRGDDVCWLHAIFRAKT 421

Query: 136 AGGPGGGVTVRNS 139
           AGGPGGGVT   S
Sbjct: 422 AGGPGGGVTPSKS 424

BLAST of Spg031003 vs. ExPASy TrEMBL
Match: A0A5D3DR18 (Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold190G00320 PE=4 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 1.3e-36
Identity = 86/133 (64.66%), Postives = 94/133 (70.68%), Query Frame = 0

Query: 16  WPRGNRSSLQTGELDKTPEAIWYRVHTGYIPLSTLSVLRDNDAVVEIELPVPDTLPTSAE 75
           WPRGNR  LQTGELDK              PLSTLSVLRDNDAVVEIELP+PDTLPTSAE
Sbjct: 302 WPRGNRPGLQTGELDKIV----------IFPLSTLSVLRDNDAVVEIELPLPDTLPTSAE 361

Query: 76  SSRTSSSTWVELYIESVHIVNVLFNVS----------VGSTGIVRGDDVCWLHAVFRAKL 135
           SS ++SSTW+ELY ESVH+  + +  S           GS GIVRGDDVCWLHA+FRAK 
Sbjct: 362 SSGSNSSTWLELYFESVHVEMLFYKPSDARAASYVCWEGSAGIVRGDDVCWLHAIFRAKT 421

Query: 136 AGGPGGGVTVRNS 139
           AGGPGGGVT   S
Sbjct: 422 AGGPGGGVTPSKS 424

BLAST of Spg031003 vs. ExPASy TrEMBL
Match: A0A5D3CJX3 (CCHC-type domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold152G00520 PE=4 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 4.9e-36
Identity = 90/137 (65.69%), Postives = 99/137 (72.26%), Query Frame = 0

Query: 15  LWPRGNRSSLQTGELDKTPEAIWYRVHTGYIPLSTLSVLRDNDAVVEIELPVPDTLPTSA 74
           L PRGNR SLQTGELDKT              LSTLSVLRDN+AV  IELPVPDTLPTSA
Sbjct: 61  LGPRGNRLSLQTGELDKTV----------IFSLSTLSVLRDNNAV--IELPVPDTLPTSA 120

Query: 75  ESSRTSSSTWVELYIESVHI-------------VNVLFNVSVGSTGIVRGDDVCWLHAVF 134
           ESS ++SSTW+ELY ESVH+             ++++FNVSVGSTGIVRGDDVCWLHAVF
Sbjct: 121 ESSGSNSSTWLELYFESVHVEMFCSFTNQVMSELHLVFNVSVGSTGIVRGDDVCWLHAVF 180

Query: 135 RAKLAGGPGGGVTVRNS 139
           RAK  GGPGGGVT   S
Sbjct: 181 RAKTVGGPGGGVTTWES 185

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0062409.11.1e-3765.22reverse transcriptase [Cucumis melo var. makuwa][more]
TYK26572.11.1e-3765.22putative Retrotransposon protein [Cucumis melo var. makuwa][more]
TYK25935.12.7e-3664.66gag/pol protein [Cucumis melo var. makuwa][more]
KAA0040194.12.7e-3664.66gag/pol protein [Cucumis melo var. makuwa][more]
TYK11835.11.0e-3565.69uncharacterized protein E5676_scaffold152G00520 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5A7V9N35.2e-3865.22Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold15... [more]
A0A5D3DSY75.2e-3865.22Putative Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A5A7T9P71.3e-3664.66Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1164G000... [more]
A0A5D3DR181.3e-3664.66Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold190G0032... [more]
A0A5D3CJX34.9e-3665.69CCHC-type domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
Match NameE-valueIdentityDescription
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg031003.1Spg031003.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015074 DNA integration
biological_process GO:0006278 RNA-dependent DNA biosynthetic process
molecular_function GO:0004518 nuclease activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0003964 RNA-directed DNA polymerase activity