Spg030945 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg030945
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionCRAL-TRIO domain-containing protein
Locationscaffold11: 28625745 .. 28628474 (-)
RNA-Seq ExpressionSpg030945
SyntenySpg030945
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGACTCCTTTATCGAAGTCCGAAGAGCGTAAGGCAACGTCGACGGGAAAGTCAGAGTGGACGGATGAGGAAGATGAGTTCAAGGAGTGTGTTGAAGAACCAAATGGTTCTGTCACGCCCCTGTTCGAAGATAGCTTGGAGAACAATGAGGTTGAGAAACAGAAGGTCGCAATCATGAGAGCTTTCGTTGAAAGAGAAGACTCCACTGCTAAGGTTACTTACTTACTCCTCTTTAATTTACTGATGCGAAAAAGATGGAGAAAATTTCGCTTTCCTGCTTTACTTTTTGTTGCGATTGTTTTGTTTCATTCAGAATCGGTTGGCTTGATTGTGTGTTTGTTCTGTTGTCTCAAATCCGTGCTCTTGTGTTTCTTTTGCAATTTTTGGTTCTTACTGGAAAGATCTCGTGGATTGTTTTGTCGAGAAATTTTTCACCACATCTTGCATCAATGGCCGGATTTGATCTTTTCTATTTTCCTGTTATTCTGTTTATGTTCATTTTGCTCGGGTTCCGTCCAACATTTCCACGAGATCAATATGTTTTTGCCCCTTTTAACTTATTTCAGAGGTAACTATGAATTACGCTCTTGCATTCGCCAAATCTACAGCTCATGGTATTGTCGATGGTGTTGTTAAAAGTAATTTTAAAATCCTGAGGTGAATTGTCCTTTTATTTTTCAATTAATTTGGCCATTGCTTTTACTATAATTGGCCCACAGAAAATTAAATCAGAGTTTTTAAAACCATTTTCAAAATATGTAGGTACAATTTGCAGCTTTTGCGGGCTATTCATATTTTTAAATTATCCAAACCTAGAGGAAGAGCAATTTGGTATAAGAAAATAAAGTGAAATGGAAATTGTGGCCAGTGATTCATCTGTAGGGATATCTGTTATGAAAAGAGAGGGTAAACTTTACCAGACATAGGCCACCTAACTTAACAAGCCATTTATTTCTGGCATTGAAGAACCTGTGCGGATAATTGGATGAATTGCAATTGTAAGATAAACATGAAATTTTCCATACGACTAGCTAATTTCAAGCTGCACAGTGGGTGGGTATTTGCTACAGTTGCTTTTTAGTATTTGCTTTAACTTAAGAAAATCATGTGCATGCAAATAATACTTGGTAACTTTTTTCCTTTTAGCGTCTTTCACATTGTGCATCATGTTTGCAGGATGTGGATGATTTCATGATACGAAGGTTTCTCCGAGCTCGTGATTTAGATATAGAGAAGGCTTCTGCCATGTTTCTGAAGTACTTGAGCTGGAGAAGATCAGCTATTCCTAATGGATTTATATCAGCGTCTGAGATAGCAACCAATCTCGCTCACAACAAGTTGTTTATGCAAGGTGTTGATAAGAAGGGACGTCCAATTGTAGTCGGCTATGGTAACAGGCACAAACAAGGAAATATAGAAGAGTTTATTCGTATGTTATGACTTTGAGCTCATGTGAATCCAAATTTGAAGCCTCTCTTATTTGTTTTACTCAGTTATCACAGATGAGGCTTCTCAGTTTATCTTTTACCTTTTGCAGGTTATGTAATCTTTGCTCTAGAGAAAATATGTTCCAGGTAATTTATGCTCCTCAAATATTTCAATTGCCTTGCATTTTAGTTGATTCCAATGGAAAATAGTACCTTGAAGGAGAGAAAAGGAATCTGGAAAGTCATAAAATATCCTGGAAATTTTAGGAGAATATATCTTTAGGTGAAATCTTGAAAAAGTTAAATCTTCATTCACCATGATTACCCCCAAACATCTTCTAACAACATAGATTACAGAGTCAATGACGAAACCTTTATTATTATTTTTTATTTTTCATTTTGTGAGAAAAAGAGGTTGAAAATAAGTCAATCCTTGTGTAGATTTCAAGTAAACCTTGATTTATGTCTTTTTTCTTCTGTGTTTCAGGATGCCAAGAGGGCAGGAAAAGTTCGTGTGTATTGGCGACCTTCAAGGATGGGGATACTCTCATAGCGATATTCGTGGATATCGAGCATCTCTATCAATCTTGCAGGTTTGACATTTGAACATCAGGAGTCCTTTAAAGCTGTGTTCAAAACATTGACGTATAAAAAATTGTTTGTTAAAAGGGATGCACCACCTCACTATTTTGTAACTCTCAAGACATTAAGAATAGCAGTGATTCACTGTTAGTAAGATTATAGAATTGCATATCAAATGGTGTAACAGTTGTTTGTGATTGTTTTGATCACAGGATTGCTACCCAGAGAGGCTGGGCAAGTTATACATCGTCCATGTCCCTTACATATTCATGACGGCATGGAAGATGGTTTACCCATTTATTGACAAGAAAACCAAGAAGAAGGTAAATTTTCTATTGCGTCTCCTGAAGTAGTTTCCTGTGTACATGAGTAGGGACATCATTTACTATCAGTCAATTACCTAATGCCACAGAATTTGATCTTAAAATAGATGGTCGTTAGAAGTATTTCTAACCAATTAAAAGTAGGTAAAACACACAAGAAGTGACCTTAAAAGGCTTTAATTAATTAAAACTTTTAAAGACGCCTGTCGAGAACCATCGAAAAACCATTCTAAACACAACATTTGGCAGGTTTAAACTGTGTTGTGCTGTGTTTGTAGATAAGCTTTGTGGAGGACAAGAAGCTGAGATCCACACTGCTTAGTGACATTGATGAGAGCCAGCTCCCTGATGTATATGGGGGCAAACTTTCGCTAGTTCCAATCCAAGACTGCTAA

mRNA sequence

ATGGCGACTCCTTTATCGAAGTCCGAAGAGCGTAAGGCAACGTCGACGGGAAAGTCAGAGTGGACGGATGAGGAAGATGAGTTCAAGGAGTGTGTTGAAGAACCAAATGGTTCTGTCACGCCCCTGTTCGAAGATAGCTTGGAGAACAATGAGGTTGAGAAACAGAAGGTCGCAATCATGAGAGCTTTCGTTGAAAGAGAAGACTCCACTGCTAAGGTAACTATGAATTACGCTCTTGCATTCGCCAAATCTACAGCTCATGGTATTGTCGATGGTGATGTGGATGATTTCATGATACGAAGGTTTCTCCGAGCTCGTGATTTAGATATAGAGAAGGCTTCTGCCATGTTTCTGAAGTACTTGAGCTGGAGAAGATCAGCTATTCCTAATGGATTTATATCAGCGTCTGAGATAGCAACCAATCTCGCTCACAACAAGTTGTTTATGCAAGGTGTTGATAAGAAGGGACGTCCAATTGTAGTCGGCTATGGTTATGTAATCTTTGCTCTAGAGAAAATATGTTCCAGGATGCCAAGAGGGCAGGAAAAGTTCGTGTGTATTGGCGACCTTCAAGGATGGGGATACTCTCATAGCGATATTCGTGGATATCGAGCATCTCTATCAATCTTGCAGGATTGCTACCCAGAGAGGCTGGGCAAGTTATACATCGTCCATGTCCCTTACATATTCATGACGGCATGGAAGATGGTTTACCCATTTATTGACAAGAAAACCAAGAAGAAGATAAGCTTTGTGGAGGACAAGAAGCTGAGATCCACACTGCTTAGTGACATTGATGAGAGCCAGCTCCCTGATGTATATGGGGGCAAACTTTCGCTAGTTCCAATCCAAGACTGCTAA

Coding sequence (CDS)

ATGGCGACTCCTTTATCGAAGTCCGAAGAGCGTAAGGCAACGTCGACGGGAAAGTCAGAGTGGACGGATGAGGAAGATGAGTTCAAGGAGTGTGTTGAAGAACCAAATGGTTCTGTCACGCCCCTGTTCGAAGATAGCTTGGAGAACAATGAGGTTGAGAAACAGAAGGTCGCAATCATGAGAGCTTTCGTTGAAAGAGAAGACTCCACTGCTAAGGTAACTATGAATTACGCTCTTGCATTCGCCAAATCTACAGCTCATGGTATTGTCGATGGTGATGTGGATGATTTCATGATACGAAGGTTTCTCCGAGCTCGTGATTTAGATATAGAGAAGGCTTCTGCCATGTTTCTGAAGTACTTGAGCTGGAGAAGATCAGCTATTCCTAATGGATTTATATCAGCGTCTGAGATAGCAACCAATCTCGCTCACAACAAGTTGTTTATGCAAGGTGTTGATAAGAAGGGACGTCCAATTGTAGTCGGCTATGGTTATGTAATCTTTGCTCTAGAGAAAATATGTTCCAGGATGCCAAGAGGGCAGGAAAAGTTCGTGTGTATTGGCGACCTTCAAGGATGGGGATACTCTCATAGCGATATTCGTGGATATCGAGCATCTCTATCAATCTTGCAGGATTGCTACCCAGAGAGGCTGGGCAAGTTATACATCGTCCATGTCCCTTACATATTCATGACGGCATGGAAGATGGTTTACCCATTTATTGACAAGAAAACCAAGAAGAAGATAAGCTTTGTGGAGGACAAGAAGCTGAGATCCACACTGCTTAGTGACATTGATGAGAGCCAGCTCCCTGATGTATATGGGGGCAAACTTTCGCTAGTTCCAATCCAAGACTGCTAA

Protein sequence

MATPLSKSEERKATSTGKSEWTDEEDEFKECVEEPNGSVTPLFEDSLENNEVEKQKVAIMRAFVEREDSTAKVTMNYALAFAKSTAHGIVDGDVDDFMIRRFLRARDLDIEKASAMFLKYLSWRRSAIPNGFISASEIATNLAHNKLFMQGVDKKGRPIVVGYGYVIFALEKICSRMPRGQEKFVCIGDLQGWGYSHSDIRGYRASLSILQDCYPERLGKLYIVHVPYIFMTAWKMVYPFIDKKTKKKISFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQDC
Homology
BLAST of Spg030945 vs. NCBI nr
Match: KAG6597698.1 (Phosphatidylinositol transfer protein 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 478.4 bits (1230), Expect = 4.3e-131
Identity = 249/302 (82.45%), Postives = 258/302 (85.43%), Query Frame = 0

Query: 1   MATPLSKSEERKATSTGKSEWT---DEEDEFKECVEEPNGSVTPLFEDSLENNEVEKQKV 60
           MATPLS+SEER  TS  KS WT   +EEDEFKEC+EEPNGSVTP+FEDSLE+NEVEKQKV
Sbjct: 1   MATPLSRSEERNVTSMEKSAWTEQEEEEDEFKECLEEPNGSVTPVFEDSLESNEVEKQKV 60

Query: 61  AIMRAFVEREDSTAKVTMNYALAFAKSTAHGIVDGDVDDFMIRRFLRARDLDIEKASAMF 120
            IMRAFVEREDS+AK                    DVDDFMIRRFLRARDLDIEKASAMF
Sbjct: 61  VIMRAFVEREDSSAK--------------------DVDDFMIRRFLRARDLDIEKASAMF 120

Query: 121 LKYLSWRRSAIPNGFISASEIATNLAHNKLFMQGVDKKGRPIVVGYG------------- 180
           LKYLSWR+SAIPNGFISASEI+TNLAHNKLFMQGVDKKGRPIVVGYG             
Sbjct: 121 LKYLSWRKSAIPNGFISASEISTNLAHNKLFMQGVDKKGRPIVVGYGNRHKQGNIEEFIR 180

Query: 181 YVIFALEKICSRMPRGQEKFVCIGDLQGWGYSHSDIRGYRASLSILQDCYPERLGKLYIV 240
           YVIFALEKICSRMPRGQEKFVCIGDLQGWGYS+SDIRGYRASLSILQDCYPERLGKLYIV
Sbjct: 181 YVIFALEKICSRMPRGQEKFVCIGDLQGWGYSNSDIRGYRASLSILQDCYPERLGKLYIV 240

Query: 241 HVPYIFMTAWKMVYPFIDKKTKKKISFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQ 287
           HVPYIFMTAWKMVYPFIDK TKKKI FVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQ
Sbjct: 241 HVPYIFMTAWKMVYPFIDKNTKKKIKFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQ 282

BLAST of Spg030945 vs. NCBI nr
Match: XP_023539819.1 (phosphatidylinositol transfer protein 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 478.4 bits (1230), Expect = 4.3e-131
Identity = 249/302 (82.45%), Postives = 258/302 (85.43%), Query Frame = 0

Query: 1   MATPLSKSEERKATSTGKSEWT---DEEDEFKECVEEPNGSVTPLFEDSLENNEVEKQKV 60
           MATPLS+SEER  TS  KS WT   +EEDEFKEC+EEPNGSVTP+FEDSLE+NEVEKQKV
Sbjct: 1   MATPLSRSEERNVTSMEKSAWTEQEEEEDEFKECLEEPNGSVTPVFEDSLESNEVEKQKV 60

Query: 61  AIMRAFVEREDSTAKVTMNYALAFAKSTAHGIVDGDVDDFMIRRFLRARDLDIEKASAMF 120
            IMRAFVEREDS+AK                    DVDDFMIRRFLRARDLDIEKASAMF
Sbjct: 61  VIMRAFVEREDSSAK--------------------DVDDFMIRRFLRARDLDIEKASAMF 120

Query: 121 LKYLSWRRSAIPNGFISASEIATNLAHNKLFMQGVDKKGRPIVVGYG------------- 180
           LKYLSWR+SAIPNGFISASEI+TNLAHNKLFMQGVDKKGRPIVVGYG             
Sbjct: 121 LKYLSWRKSAIPNGFISASEISTNLAHNKLFMQGVDKKGRPIVVGYGNRHKQGNMEEFIR 180

Query: 181 YVIFALEKICSRMPRGQEKFVCIGDLQGWGYSHSDIRGYRASLSILQDCYPERLGKLYIV 240
           YVIFALEKICSRMPRGQEKFVCIGDLQGWGYS+SDIRGYRASLSILQDCYPERLGKLYIV
Sbjct: 181 YVIFALEKICSRMPRGQEKFVCIGDLQGWGYSNSDIRGYRASLSILQDCYPERLGKLYIV 240

Query: 241 HVPYIFMTAWKMVYPFIDKKTKKKISFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQ 287
           HVPYIFMTAWKMVYPFIDK TKKKI FVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQ
Sbjct: 241 HVPYIFMTAWKMVYPFIDKNTKKKIKFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQ 282

BLAST of Spg030945 vs. NCBI nr
Match: KAG7029143.1 (Random slug protein 5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 477.2 bits (1227), Expect = 9.6e-131
Identity = 248/302 (82.12%), Postives = 258/302 (85.43%), Query Frame = 0

Query: 1   MATPLSKSEERKATSTGKSEWT---DEEDEFKECVEEPNGSVTPLFEDSLENNEVEKQKV 60
           MATPLS+SEER  TS  KS WT   +EEDEFKEC+EEPNGSVTP+FEDSLE+NEVEKQKV
Sbjct: 1   MATPLSRSEERNVTSMEKSAWTEQEEEEDEFKECLEEPNGSVTPVFEDSLESNEVEKQKV 60

Query: 61  AIMRAFVEREDSTAKVTMNYALAFAKSTAHGIVDGDVDDFMIRRFLRARDLDIEKASAMF 120
            IMRAFVEREDS+AK                    DVDDFMIRRFLRARDLDIEKASAMF
Sbjct: 61  VIMRAFVEREDSSAK--------------------DVDDFMIRRFLRARDLDIEKASAMF 120

Query: 121 LKYLSWRRSAIPNGFISASEIATNLAHNKLFMQGVDKKGRPIVVGYG------------- 180
           LKYLSWR+SAIPNGFISASEI+TNLAHNKLFMQGVDKKGRPIVVGYG             
Sbjct: 121 LKYLSWRKSAIPNGFISASEISTNLAHNKLFMQGVDKKGRPIVVGYGNRHKQGNIEEFIR 180

Query: 181 YVIFALEKICSRMPRGQEKFVCIGDLQGWGYSHSDIRGYRASLSILQDCYPERLGKLYIV 240
           YVIFALEKICSRMPRGQEKFVCIGDLQGWGYS+SDIRGYRASLSILQDCYPERLGKLYIV
Sbjct: 181 YVIFALEKICSRMPRGQEKFVCIGDLQGWGYSNSDIRGYRASLSILQDCYPERLGKLYIV 240

Query: 241 HVPYIFMTAWKMVYPFIDKKTKKKISFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQ 287
           HVPYIFMTAWKMVYPFIDK TKKKI FVEDKKLRSTLLSDIDESQLPDVYGGKL+LVPIQ
Sbjct: 241 HVPYIFMTAWKMVYPFIDKNTKKKIKFVEDKKLRSTLLSDIDESQLPDVYGGKLALVPIQ 282

BLAST of Spg030945 vs. NCBI nr
Match: XP_022932698.1 (phosphatidylinositol transfer protein 3 [Cucurbita moschata])

HSP 1 Score: 473.4 bits (1217), Expect = 1.4e-129
Identity = 247/301 (82.06%), Postives = 256/301 (85.05%), Query Frame = 0

Query: 1   MATPLSKSEERKATSTGKSEWT---DEEDEFKECVEEPNGSVTPLFEDSLENNEVEKQKV 60
           MATPLS+SEER  TS  KS WT   +EEDEFKEC+EEPNGSVTP+FEDSLE+NEVEKQKV
Sbjct: 1   MATPLSRSEERNVTSMEKSAWTEQEEEEDEFKECLEEPNGSVTPVFEDSLESNEVEKQKV 60

Query: 61  AIMRAFVEREDSTAKVTMNYALAFAKSTAHGIVDGDVDDFMIRRFLRARDLDIEKASAMF 120
            IMRAFVEREDS+AK                    DVDDFMIRRFLRARDLDIEKASAMF
Sbjct: 61  VIMRAFVEREDSSAK--------------------DVDDFMIRRFLRARDLDIEKASAMF 120

Query: 121 LKYLSWRRSAIPNGFISASEIATNLAHNKLFMQGVDKKGRPIVVGYG------------- 180
           LKYLSWR+S IPNGFISASEI+TNLAHNKLFMQGVDKKGRPIVVGYG             
Sbjct: 121 LKYLSWRKSVIPNGFISASEISTNLAHNKLFMQGVDKKGRPIVVGYGNRHKQGNIEEFIR 180

Query: 181 YVIFALEKICSRMPRGQEKFVCIGDLQGWGYSHSDIRGYRASLSILQDCYPERLGKLYIV 240
           YVIFALEKICSRMPRGQEKFVCIGDLQGWGYS+SDIRGYRASLSILQDCYPERLGKLYIV
Sbjct: 181 YVIFALEKICSRMPRGQEKFVCIGDLQGWGYSNSDIRGYRASLSILQDCYPERLGKLYIV 240

Query: 241 HVPYIFMTAWKMVYPFIDKKTKKKISFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQ 286
           HVPYIFMTAWKMVYPFIDK TKKKI FVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQ
Sbjct: 241 HVPYIFMTAWKMVYPFIDKNTKKKIKFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQ 281

BLAST of Spg030945 vs. NCBI nr
Match: XP_022972040.1 (phosphatidylinositol transfer protein 3-like [Cucurbita maxima])

HSP 1 Score: 470.7 bits (1210), Expect = 9.0e-129
Identity = 244/302 (80.79%), Postives = 256/302 (84.77%), Query Frame = 0

Query: 1   MATPLSKSEERKATSTGKSEWT---DEEDEFKECVEEPNGSVTPLFEDSLENNEVEKQKV 60
           MA PLS+SEER  TS  KS WT   +EEDEFKEC+EEPNGSVTP+FEDSLE+NEVEKQKV
Sbjct: 1   MAIPLSRSEERNVTSMEKSAWTEQEEEEDEFKECLEEPNGSVTPVFEDSLESNEVEKQKV 60

Query: 61  AIMRAFVEREDSTAKVTMNYALAFAKSTAHGIVDGDVDDFMIRRFLRARDLDIEKASAMF 120
            IMRAFVEREDS+AK                    DVDDFMIRRFLRARDLD+EKASAMF
Sbjct: 61  VIMRAFVEREDSSAK--------------------DVDDFMIRRFLRARDLDVEKASAMF 120

Query: 121 LKYLSWRRSAIPNGFISASEIATNLAHNKLFMQGVDKKGRPIVVGYG------------- 180
           LKYLSWR++AIPNGFISASEI+TNLAHNKLFMQGVDKKGRPIVVGYG             
Sbjct: 121 LKYLSWRKAAIPNGFISASEISTNLAHNKLFMQGVDKKGRPIVVGYGNRHKQGNIEEFIR 180

Query: 181 YVIFALEKICSRMPRGQEKFVCIGDLQGWGYSHSDIRGYRASLSILQDCYPERLGKLYIV 240
           YVIFALEKICSRMPRGQEKFVCIGDLQGWGYS+SDIRGYRASLSILQDCYPERLGKLYIV
Sbjct: 181 YVIFALEKICSRMPRGQEKFVCIGDLQGWGYSNSDIRGYRASLSILQDCYPERLGKLYIV 240

Query: 241 HVPYIFMTAWKMVYPFIDKKTKKKISFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQ 287
           HVP+IF TAWKMVYPFIDK TKKKI FVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQ
Sbjct: 241 HVPFIFTTAWKMVYPFIDKNTKKKIKFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQ 282

BLAST of Spg030945 vs. ExPASy Swiss-Prot
Match: Q10137 (Sec14 cytosolic factor OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=sec14 PE=4 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 1.3e-16
Identity = 67/215 (31.16%), Postives = 100/215 (46.51%), Query Frame = 0

Query: 94  VDDFMIRRFLRARDLDIEKASAMFLKYLSWRRSAIPNGFISASEIATNLAHNKLFMQ--- 153
           +DD  + RFLRAR  +++++  MF+K   WR+    +  I         A +K + Q   
Sbjct: 48  LDDATLLRFLRARKFNLQQSLEMFIKCEKWRKEFGVDDLIKNFHYDEKEAVSKYYPQFYH 107

Query: 154 GVDKKGRPIVVG------------------------YGYVIFALEKI--CSRMPRGQEKF 213
             D  GRP+ V                         Y Y + AL++   CSR   G  + 
Sbjct: 108 KTDIDGRPVYVEQLGNIDLKKLYQITTPERMMQNLVYEYEMLALKRFPACSRKAGGLIET 167

Query: 214 VC-IGDLQGWGYS--HSDIRGYRASLSILQDCYPERLGKLYIVHVPYIFMTAWKMVYPFI 273
            C I DL+G G +  HS     R + SI QD YPER+GK Y+++ P+ F +A+ ++  F+
Sbjct: 168 SCTIMDLKGVGITSIHSVYSYIRQASSISQDYYPERMGKFYVINAPWGFSSAFNLIKGFL 227

Query: 274 DKKTKKKISFVEDKKLRSTLLSDIDESQLPDVYGG 277
           D+ T KKI  +     +S LL  I    LP   GG
Sbjct: 228 DEATVKKIHIL-GSNYKSALLEQIPADNLPAKLGG 261

BLAST of Spg030945 vs. ExPASy Swiss-Prot
Match: Q55CU8 (Phosphatidylinositol transfer protein 3 OS=Dictyostelium discoideum OX=44689 GN=pitC PE=2 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 1.1e-15
Identity = 79/300 (26.33%), Postives = 131/300 (43.67%), Query Frame = 0

Query: 1   MATPLSKSEERKATSTGKSEWTDEEDEFKECVEEPNGSVTPLFEDSLENNEVEKQKVAIM 60
           M +  S S+   A +TG SE  D   +    + +      PL       N  EKQ    +
Sbjct: 1   MESTASTSDSASAITTGLSE-VDINQQSPPIILKEGEIFDPL------KNLNEKQ----L 60

Query: 61  RAFVEREDSTAKVTMNYALAFAKSTAHGIVDGDVDDFMIRRFLRARDLDIEKASAMFLKY 120
            AF E + + + +T    +AF              D    R+LRAR+  + K+  M    
Sbjct: 61  EAFKEIKSNFSDLTDPTDIAFC------------TDMCFLRYLRARNYIVSKSEKMLRDT 120

Query: 121 LSWRRSAIPNGFISASEIATNLAHNKLFMQGVDKKGRPIV---------------VGYGY 180
           L WR+   P       +I    +   +++   DKKGRPI+               + +  
Sbjct: 121 LEWRKKFRPQDIQLGGDIREIGSAGCVYVNKRDKKGRPIIFAVPRNDTLKNVPSELKFKN 180

Query: 181 VIFALEKICSRM--PRGQEKFVCIGDLQGWGYSHSDIRGYRASLSILQDCYPERLGKLYI 240
           +++ LE+  SRM  P+G E+F  I D + +G  + D++    ++  L D  PER+G+   
Sbjct: 181 LVYWLEQGFSRMDEPKGIEQFCFIVDYKDFGSGNMDMKTNLEAMHFLLDHCPERMGQSLF 240

Query: 241 VHVPYIFMTAWKMVYPFIDKKTKKKISFVEDKKLR-----STLLSDIDESQLPDVYGGKL 279
           +  P +F  AWK++ PF+++ T  K+ F+  KK+      + LL  +D   L    GG L
Sbjct: 241 LDPPALFWFAWKIISPFLNEVTLSKVRFINSKKVDGKRTFAELLEYVDIENLEQNLGGNL 277

BLAST of Spg030945 vs. ExPASy Swiss-Prot
Match: P33324 (CRAL-TRIO domain-containing protein YKL091C OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YKL091C PE=1 SV=2)

HSP 1 Score: 80.9 bits (198), Expect = 2.6e-14
Identity = 69/221 (31.22%), Postives = 99/221 (44.80%), Query Frame = 0

Query: 94  VDDFMIRRFLRARDLDIEKASAMFLKYLSWRRSAIPNGFI------SASEIATNLAHNKL 153
           +DD  + RFLRAR  DI  +  MF++   WR     N  I        +E    +   K+
Sbjct: 49  LDDSTLLRFLRARKFDINASVEMFVETERWREEYGANTIIEDYENNKEAEDKERIKLAKM 108

Query: 154 FMQ---GVDKKGRPIV------------------------VGYGYVIFALEKI--CSRMP 213
           + Q    VDK GRP+                         +   Y +FA  ++  CSR  
Sbjct: 109 YPQYYHHVDKDGRPLYFEELGGINLKKMYKITTEKQMLRNLVKEYELFATYRVPACSRRA 168

Query: 214 RGQEKFVC-IGDLQGWGYSHS-DIRGYRASLS-ILQDCYPERLGKLYIVHVPYIFMTAWK 273
               +  C + DL+G   S++  +  Y   ++ I Q+ YPER+GK YI+H P+ F T +K
Sbjct: 169 GYLIETSCTVLDLKGISLSNAYHVLSYIKDVADISQNYYPERMGKFYIIHSPFGFSTMFK 228

Query: 274 MVYPFIDKKTKKKISFVEDKKLRSTLLSDIDESQLPDVYGG 277
           MV PF+D  T  KI F+     +  LL  I    LP  YGG
Sbjct: 229 MVKPFLDPVTVSKI-FILGSSYKKELLKQIPIENLPVKYGG 268

BLAST of Spg030945 vs. ExPASy Swiss-Prot
Match: P58875 (SEC14-like protein 2 OS=Bos taurus OX=9913 GN=SEC14L2 PE=1 SV=2)

HSP 1 Score: 77.4 bits (189), Expect = 2.9e-13
Identity = 61/214 (28.50%), Postives = 99/214 (46.26%), Query Frame = 0

Query: 95  DDFMIRRFLRARDLDIEKASAMFLKYLSWRRSAIPNGFISASEIATNLAHNKLFMQGVDK 154
           DD+ + R+LRAR+ +++K+ AM  K++ +R+    +  +S         +    M G D 
Sbjct: 34  DDYFLLRWLRARNFNLQKSEAMLRKHVEFRKQKDIDNIMSWQPPEVVQQYLSGGMCGYDL 93

Query: 155 KGRPI---VVG----YGYVIFA---------------LEKICSR----MPRGQEKFVCIG 214
           +G PI   ++G     G ++ A               L + C R    M +  E    I 
Sbjct: 94  EGSPIWYDIIGPLDAKGLLLSASKQDLFKTKMRDCELLLQECVRQTEKMGKKIEATTLIY 153

Query: 215 DLQGWGYSH---SDIRGYRASLSILQDCYPERLGKLYIVHVPYIFMTAWKMVYPFIDKKT 274
           D +G G  H     +  Y   L + ++ YPE L +L+IV  P +F  A+ +V PF+ + T
Sbjct: 154 DCEGLGLKHLWKPAVEAYGEFLCMFEENYPETLKRLFIVKAPKLFPVAYNLVKPFLSEDT 213

Query: 275 KKKISFVEDKKLRSTLLSDIDESQLPDVYGGKLS 280
           +KKI  V     +  LL  I   QLP  YGG ++
Sbjct: 214 RKKIQ-VLGANWKEVLLKYISPDQLPVEYGGTMT 246

BLAST of Spg030945 vs. ExPASy Swiss-Prot
Match: O76054 (SEC14-like protein 2 OS=Homo sapiens OX=9606 GN=SEC14L2 PE=1 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 2.9e-13
Identity = 56/214 (26.17%), Postives = 96/214 (44.86%), Query Frame = 0

Query: 95  DDFMIRRFLRARDLDIEKASAMFLKYLSWRRSAIPNGFISASEIATNLAHNKLFMQGVDK 154
           DD+ + R+LRAR  D++K+ AM  K++ +R+    +  IS         +    M G D 
Sbjct: 34  DDYFLLRWLRARSFDLQKSEAMLRKHVEFRKQKDIDNIISWQPPEVIQQYLSGGMCGYDL 93

Query: 155 KGRPI---VVG---YGYVIFALEK--------------------ICSRMPRGQEKFVCIG 214
            G P+   ++G      ++F+  K                      +++ R  E    I 
Sbjct: 94  DGCPVWYDIIGPLDAKGLLFSASKQDLLRTKMRECELLLQECAHQTTKLGRKVETITIIY 153

Query: 215 DLQGWGYSH---SDIRGYRASLSILQDCYPERLGKLYIVHVPYIFMTAWKMVYPFIDKKT 274
           D +G G  H     +  Y   L + ++ YPE L +L++V  P +F  A+ ++ PF+ + T
Sbjct: 154 DCEGLGLKHLWKPAVEAYGEFLCMFEENYPETLKRLFVVKAPKLFPVAYNLIKPFLSEDT 213

Query: 275 KKKISFVEDKKLRSTLLSDIDESQLPDVYGGKLS 280
           +KKI  V     +  LL  I   Q+P  YGG ++
Sbjct: 214 RKKI-MVLGANWKEVLLKHISPDQVPVEYGGTMT 246

BLAST of Spg030945 vs. ExPASy TrEMBL
Match: A0A6J1F2H3 (phosphatidylinositol transfer protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111439167 PE=4 SV=1)

HSP 1 Score: 473.4 bits (1217), Expect = 6.7e-130
Identity = 247/301 (82.06%), Postives = 256/301 (85.05%), Query Frame = 0

Query: 1   MATPLSKSEERKATSTGKSEWT---DEEDEFKECVEEPNGSVTPLFEDSLENNEVEKQKV 60
           MATPLS+SEER  TS  KS WT   +EEDEFKEC+EEPNGSVTP+FEDSLE+NEVEKQKV
Sbjct: 1   MATPLSRSEERNVTSMEKSAWTEQEEEEDEFKECLEEPNGSVTPVFEDSLESNEVEKQKV 60

Query: 61  AIMRAFVEREDSTAKVTMNYALAFAKSTAHGIVDGDVDDFMIRRFLRARDLDIEKASAMF 120
            IMRAFVEREDS+AK                    DVDDFMIRRFLRARDLDIEKASAMF
Sbjct: 61  VIMRAFVEREDSSAK--------------------DVDDFMIRRFLRARDLDIEKASAMF 120

Query: 121 LKYLSWRRSAIPNGFISASEIATNLAHNKLFMQGVDKKGRPIVVGYG------------- 180
           LKYLSWR+S IPNGFISASEI+TNLAHNKLFMQGVDKKGRPIVVGYG             
Sbjct: 121 LKYLSWRKSVIPNGFISASEISTNLAHNKLFMQGVDKKGRPIVVGYGNRHKQGNIEEFIR 180

Query: 181 YVIFALEKICSRMPRGQEKFVCIGDLQGWGYSHSDIRGYRASLSILQDCYPERLGKLYIV 240
           YVIFALEKICSRMPRGQEKFVCIGDLQGWGYS+SDIRGYRASLSILQDCYPERLGKLYIV
Sbjct: 181 YVIFALEKICSRMPRGQEKFVCIGDLQGWGYSNSDIRGYRASLSILQDCYPERLGKLYIV 240

Query: 241 HVPYIFMTAWKMVYPFIDKKTKKKISFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQ 286
           HVPYIFMTAWKMVYPFIDK TKKKI FVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQ
Sbjct: 241 HVPYIFMTAWKMVYPFIDKNTKKKIKFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQ 281

BLAST of Spg030945 vs. ExPASy TrEMBL
Match: A0A6J1I7E9 (phosphatidylinositol transfer protein 3-like OS=Cucurbita maxima OX=3661 GN=LOC111470692 PE=4 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 4.3e-129
Identity = 244/302 (80.79%), Postives = 256/302 (84.77%), Query Frame = 0

Query: 1   MATPLSKSEERKATSTGKSEWT---DEEDEFKECVEEPNGSVTPLFEDSLENNEVEKQKV 60
           MA PLS+SEER  TS  KS WT   +EEDEFKEC+EEPNGSVTP+FEDSLE+NEVEKQKV
Sbjct: 1   MAIPLSRSEERNVTSMEKSAWTEQEEEEDEFKECLEEPNGSVTPVFEDSLESNEVEKQKV 60

Query: 61  AIMRAFVEREDSTAKVTMNYALAFAKSTAHGIVDGDVDDFMIRRFLRARDLDIEKASAMF 120
            IMRAFVEREDS+AK                    DVDDFMIRRFLRARDLD+EKASAMF
Sbjct: 61  VIMRAFVEREDSSAK--------------------DVDDFMIRRFLRARDLDVEKASAMF 120

Query: 121 LKYLSWRRSAIPNGFISASEIATNLAHNKLFMQGVDKKGRPIVVGYG------------- 180
           LKYLSWR++AIPNGFISASEI+TNLAHNKLFMQGVDKKGRPIVVGYG             
Sbjct: 121 LKYLSWRKAAIPNGFISASEISTNLAHNKLFMQGVDKKGRPIVVGYGNRHKQGNIEEFIR 180

Query: 181 YVIFALEKICSRMPRGQEKFVCIGDLQGWGYSHSDIRGYRASLSILQDCYPERLGKLYIV 240
           YVIFALEKICSRMPRGQEKFVCIGDLQGWGYS+SDIRGYRASLSILQDCYPERLGKLYIV
Sbjct: 181 YVIFALEKICSRMPRGQEKFVCIGDLQGWGYSNSDIRGYRASLSILQDCYPERLGKLYIV 240

Query: 241 HVPYIFMTAWKMVYPFIDKKTKKKISFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQ 287
           HVP+IF TAWKMVYPFIDK TKKKI FVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQ
Sbjct: 241 HVPFIFTTAWKMVYPFIDKNTKKKIKFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQ 282

BLAST of Spg030945 vs. ExPASy TrEMBL
Match: A0A6J1DXU4 (random slug protein 5 OS=Momordica charantia OX=3673 GN=LOC111025140 PE=4 SV=1)

HSP 1 Score: 447.6 bits (1150), Expect = 3.9e-122
Identity = 230/300 (76.67%), Postives = 251/300 (83.67%), Query Frame = 0

Query: 1   MATPLSKSEERKATSTGKSEWT--DEEDEFKECVEEPNGSVTPLFEDSLENNEVEKQKVA 60
           MATP SK  E KATS  KS+WT  +EEDEF+ECVE+ NGSV+P+ ED L++NE+EK+KVA
Sbjct: 1   MATPQSKIVENKATSMEKSKWTEDEEEDEFEECVEDQNGSVSPVLEDGLDSNELEKRKVA 60

Query: 61  IMRAFVEREDSTAKVTMNYALAFAKSTAHGIVDGDVDDFMIRRFLRARDLDIEKASAMFL 120
           +MRAFVERED +AK                    DVDDFMIRRFLRARDLDIEKASAMFL
Sbjct: 61  LMRAFVEREDPSAK--------------------DVDDFMIRRFLRARDLDIEKASAMFL 120

Query: 121 KYLSWRRSAIPNGFISASEIATNLAHNKLFMQGVDKKGRPIVVGYG-------------Y 180
           KYL+WRRSAIPNGFIS SEI+ N+AHNKLFMQGVDKKGRPIVVG+G             Y
Sbjct: 121 KYLTWRRSAIPNGFISLSEISNNIAHNKLFMQGVDKKGRPIVVGFGGRHKQGNMEEFIRY 180

Query: 181 VIFALEKICSRMPRGQEKFVCIGDLQGWGYSHSDIRGYRASLSILQDCYPERLGKLYIVH 240
           VIF LEKICSRMPRGQEKFVCIGDLQGWGYS+SDIRGYRASLSILQDCYPERLGKLYIVH
Sbjct: 181 VIFVLEKICSRMPRGQEKFVCIGDLQGWGYSNSDIRGYRASLSILQDCYPERLGKLYIVH 240

Query: 241 VPYIFMTAWKMVYPFIDKKTKKKISFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQD 286
           VPYIFMTAWKMVYPFIDKKTKKKISFVEDKKLRSTLLSDIDESQ+PD+YGGKL L+PI+D
Sbjct: 241 VPYIFMTAWKMVYPFIDKKTKKKISFVEDKKLRSTLLSDIDESQVPDIYGGKLQLIPIED 280

BLAST of Spg030945 vs. ExPASy TrEMBL
Match: A0A6J1J0A3 (patellin-4-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111480094 PE=4 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 1.5e-121
Identity = 229/303 (75.58%), Postives = 251/303 (82.84%), Query Frame = 0

Query: 1   MATPLSKSEERKATSTGKSEWT----DEEDEFKECVEEPNGSVTPLFEDSLENNEVEKQK 60
           MAT LS+SEER AT   K EWT    +EE EFKECVEEPNGS+TP+ +DSLE+NE++KQK
Sbjct: 1   MATQLSRSEERNATPKEKLEWTKEEEEEEKEFKECVEEPNGSLTPVIDDSLESNELDKQK 60

Query: 61  VAIMRAFVEREDSTAKVTMNYALAFAKSTAHGIVDGDVDDFMIRRFLRARDLDIEKASAM 120
           +AIMRAFVE EDS+AK                    DVDDFM+RRFLRARDLDIEKASAM
Sbjct: 61  LAIMRAFVEGEDSSAK--------------------DVDDFMLRRFLRARDLDIEKASAM 120

Query: 121 FLKYLSWRRSAIPNGFISASEIATNLAHNKLFMQGVDKKGRPIVVGYG------------ 180
           FLKYLSWRRSAIP GFIS SE++TNLAHNKLFMQGVDKKGRPIVVG+G            
Sbjct: 121 FLKYLSWRRSAIPGGFISVSELSTNLAHNKLFMQGVDKKGRPIVVGFGNRHKQGNLEEFI 180

Query: 181 -YVIFALEKICSRMPRGQEKFVCIGDLQGWGYSHSDIRGYRASLSILQDCYPERLGKLYI 240
            +VIF LEK+CSR+PRGQEKFVCIGDLQGWGYS+SDIRGYRA+LSILQDCYPERLGKLY+
Sbjct: 181 RFVIFFLEKVCSRIPRGQEKFVCIGDLQGWGYSNSDIRGYRAALSILQDCYPERLGKLYL 240

Query: 241 VHVPYIFMTAWKMVYPFIDKKTKKKISFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPI 287
           VHVPYIFMTAWKMVYPFIDKKTK KISFVEDKKL+STLL DI+ESQLPD YGGKLSLVPI
Sbjct: 241 VHVPYIFMTAWKMVYPFIDKKTKNKISFVEDKKLKSTLLDDINESQLPDAYGGKLSLVPI 283

BLAST of Spg030945 vs. ExPASy TrEMBL
Match: A0A6J1GUP1 (patellin-4-like OS=Cucurbita moschata OX=3662 GN=LOC111457645 PE=4 SV=1)

HSP 1 Score: 442.6 bits (1137), Expect = 1.3e-120
Identity = 230/306 (75.16%), Postives = 251/306 (82.03%), Query Frame = 0

Query: 1   MATPLSKSEERKATSTGKSEWTDEED-------EFKECVEEPNGSVTPLFEDSLENNEVE 60
           MA+ LS+S ER AT   KSEWT+EE+       EFKECVEEPNGS+TP+ +DSLE+NE+E
Sbjct: 1   MASQLSRSVERNATPKEKSEWTEEEEEEEEEEKEFKECVEEPNGSLTPVIDDSLESNELE 60

Query: 61  KQKVAIMRAFVEREDSTAKVTMNYALAFAKSTAHGIVDGDVDDFMIRRFLRARDLDIEKA 120
           KQK+AIMRAFVE EDS+AK                    DVDDFM+RRFLRARDLDIEKA
Sbjct: 61  KQKLAIMRAFVEGEDSSAK--------------------DVDDFMLRRFLRARDLDIEKA 120

Query: 121 SAMFLKYLSWRRSAIPNGFISASEIATNLAHNKLFMQGVDKKGRPIVVGYG--------- 180
           SAMFLKYLSWRRSAIP+GFIS SE++TNLAHNKLFMQGVDKKG PIVVG+G         
Sbjct: 121 SAMFLKYLSWRRSAIPDGFISVSELSTNLAHNKLFMQGVDKKGHPIVVGFGNRHKQGNLE 180

Query: 181 ----YVIFALEKICSRMPRGQEKFVCIGDLQGWGYSHSDIRGYRASLSILQDCYPERLGK 240
               +VIFAL+KICSR+PRGQEKFVCIGDLQGWGYS+SDIRGYRA+LSILQD YPERLGK
Sbjct: 181 EFIRFVIFALDKICSRIPRGQEKFVCIGDLQGWGYSNSDIRGYRAALSILQDYYPERLGK 240

Query: 241 LYIVHVPYIFMTAWKMVYPFIDKKTKKKISFVEDKKLRSTLLSDIDESQLPDVYGGKLSL 287
           LYIVHVPYIFMTAWKMVYPFIDKKTK KISFVEDKKL STLL DIDESQLPD YGGKLSL
Sbjct: 241 LYIVHVPYIFMTAWKMVYPFIDKKTKNKISFVEDKKLNSTLLDDIDESQLPDAYGGKLSL 286

BLAST of Spg030945 vs. TAIR 10
Match: AT1G01630.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 315.5 bits (807), Expect = 4.5e-86
Identity = 166/278 (59.71%), Postives = 191/278 (68.71%), Query Frame = 0

Query: 27  EFKECVEEPNGS----VTPLFEDSLENNEVEKQKVAIMRAFVEREDSTAKVTMNYALAFA 86
           E KE  +EP  +      PL ED     E+E+ KV IMRA  +R+D   K          
Sbjct: 2   ENKETKQEPAAAAEQKTVPLIED-----EIERSKVGIMRALCDRQDPETK---------- 61

Query: 87  KSTAHGIVDGDVDDFMIRRFLRARDLDIEKASAMFLKYLSWRRSAIPNGFISASEIATNL 146
                     +VDD MIRRFLRARDLDIEKAS MFL YL+W+RS +P G I  +EIA +L
Sbjct: 62  ----------EVDDLMIRRFLRARDLDIEKASTMFLNYLTWKRSMLPKGHIPEAEIANDL 121

Query: 147 AHNKLFMQGVDKKGRPIVVGYG---------------YVIFALEKICSRMPRGQEKFVCI 206
           +HNK+ MQG DK GRPI V  G               +V++ LEKIC+RMPRGQEKFV I
Sbjct: 122 SHNKMCMQGHDKMGRPIAVAIGNRHNPSKGNPDEFKRFVVYTLEKICARMPRGQEKFVAI 181

Query: 207 GDLQGWGYSHSDIRGYRASLSILQDCYPERLGKLYIVHVPYIFMTAWKMVYPFIDKKTKK 266
           GDLQGWGYS+ DIRGY A+LS LQDCYPERLGKLYIVH PYIFMTAWK++YPFID  TKK
Sbjct: 182 GDLQGWGYSNCDIRGYLAALSTLQDCYPERLGKLYIVHAPYIFMTAWKVIYPFIDANTKK 241

Query: 267 KISFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQD 286
           KI FVE+KKL  TLL DIDESQLPD+YGGKL LVPIQ+
Sbjct: 242 KIVFVENKKLTPTLLEDIDESQLPDIYGGKLPLVPIQE 254

BLAST of Spg030945 vs. TAIR 10
Match: AT1G14820.3 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 130.6 bits (327), Expect = 2.1e-30
Identity = 75/206 (36.41%), Postives = 106/206 (51.46%), Query Frame = 0

Query: 99  IRRFLRARDLDIEKASAMFLKYLSWRRSAI-PNGFISASEIATNLAHNKLFMQGVDKKGR 158
           + RFL AR +D  KA+ MF+ +  WR S + P GFI  SE+   L   K+ +QG  K G 
Sbjct: 31  LMRFLVARSMDPVKAAKMFVDWQKWRASMVPPTGFIPESEVQDELEFRKVCLQGPTKSGH 90

Query: 159 PIVVGY--------------GYVIFALEKICSRMPRGQ----EKFVCIGDLQGWGYSHSD 218
           P+V+                 +V++AL+K  +    G+    EK V + DL    Y + D
Sbjct: 91  PLVLVITSKHFASKDPANFKKFVVYALDKTIASGNNGKEVGGEKLVAVIDLANITYKNLD 150

Query: 219 IRGYRASLSILQDCYPERLGKLYIVHVPYIFMTAWKMVYPFIDKKTKKKISFVEDKKLRS 278
            RG       LQ  YPERL K YI+H+P  F+T WK V  F++K T++KI  V D + + 
Sbjct: 151 ARGLITGFQFLQSYYPERLAKCYILHMPGFFVTVWKFVCRFLEKATQEKIVIVTDGEEQR 210

Query: 279 TLLSDIDESQLPDVYGGKLSLVPIQD 286
               +I    LP+ YGG+  L  IQD
Sbjct: 211 KFEEEIGADALPEEYGGRAKLTAIQD 236

BLAST of Spg030945 vs. TAIR 10
Match: AT1G14820.2 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 130.6 bits (327), Expect = 2.1e-30
Identity = 75/206 (36.41%), Postives = 106/206 (51.46%), Query Frame = 0

Query: 99  IRRFLRARDLDIEKASAMFLKYLSWRRSAI-PNGFISASEIATNLAHNKLFMQGVDKKGR 158
           + RFL AR +D  KA+ MF+ +  WR S + P GFI  SE+   L   K+ +QG  K G 
Sbjct: 18  LMRFLVARSMDPVKAAKMFVDWQKWRASMVPPTGFIPESEVQDELEFRKVCLQGPTKSGH 77

Query: 159 PIVVGY--------------GYVIFALEKICSRMPRGQ----EKFVCIGDLQGWGYSHSD 218
           P+V+                 +V++AL+K  +    G+    EK V + DL    Y + D
Sbjct: 78  PLVLVITSKHFASKDPANFKKFVVYALDKTIASGNNGKEVGGEKLVAVIDLANITYKNLD 137

Query: 219 IRGYRASLSILQDCYPERLGKLYIVHVPYIFMTAWKMVYPFIDKKTKKKISFVEDKKLRS 278
            RG       LQ  YPERL K YI+H+P  F+T WK V  F++K T++KI  V D + + 
Sbjct: 138 ARGLITGFQFLQSYYPERLAKCYILHMPGFFVTVWKFVCRFLEKATQEKIVIVTDGEEQR 197

Query: 279 TLLSDIDESQLPDVYGGKLSLVPIQD 286
               +I    LP+ YGG+  L  IQD
Sbjct: 198 KFEEEIGADALPEEYGGRAKLTAIQD 223

BLAST of Spg030945 vs. TAIR 10
Match: AT1G14820.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 130.6 bits (327), Expect = 2.1e-30
Identity = 75/206 (36.41%), Postives = 106/206 (51.46%), Query Frame = 0

Query: 99  IRRFLRARDLDIEKASAMFLKYLSWRRSAI-PNGFISASEIATNLAHNKLFMQGVDKKGR 158
           + RFL AR +D  KA+ MF+ +  WR S + P GFI  SE+   L   K+ +QG  K G 
Sbjct: 18  LMRFLVARSMDPVKAAKMFVDWQKWRASMVPPTGFIPESEVQDELEFRKVCLQGPTKSGH 77

Query: 159 PIVVGY--------------GYVIFALEKICSRMPRGQ----EKFVCIGDLQGWGYSHSD 218
           P+V+                 +V++AL+K  +    G+    EK V + DL    Y + D
Sbjct: 78  PLVLVITSKHFASKDPANFKKFVVYALDKTIASGNNGKEVGGEKLVAVIDLANITYKNLD 137

Query: 219 IRGYRASLSILQDCYPERLGKLYIVHVPYIFMTAWKMVYPFIDKKTKKKISFVEDKKLRS 278
            RG       LQ  YPERL K YI+H+P  F+T WK V  F++K T++KI  V D + + 
Sbjct: 138 ARGLITGFQFLQSYYPERLAKCYILHMPGFFVTVWKFVCRFLEKATQEKIVIVTDGEEQR 197

Query: 279 TLLSDIDESQLPDVYGGKLSLVPIQD 286
               +I    LP+ YGG+  L  IQD
Sbjct: 198 KFEEEIGADALPEEYGGRAKLTAIQD 223

BLAST of Spg030945 vs. TAIR 10
Match: AT4G36640.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 90.1 bits (222), Expect = 3.1e-18
Identity = 58/204 (28.43%), Postives = 99/204 (48.53%), Query Frame = 0

Query: 96  DFMIRRFLRARDLDIEKASAMFLKYLSWRRSAIPNGF---ISASEIATNLAHNKLFMQGV 155
           D  +RRFL AR+ D+EKA  M  + L WR +  P        A E  T  A    F    
Sbjct: 41  DASLRRFLDARNWDVEKAKKMIQETLKWRSTYKPQEIRWNQVAHEGETGKASRASFH--- 100

Query: 156 DKKGRPIVV-------------GYGYVIFALEKICSRMPRGQEKFVCIGDLQGWGYS-HS 215
           D++GR +++                ++++ LE     +P+GQ++   + D  GW  + + 
Sbjct: 101 DRQGRVVLIMRPAMQNSTSQEGNIRHLVYLLENAIINLPKGQKQMSWLIDFTGWSMAVNP 160

Query: 216 DIRGYRASLSILQDCYPERLGKLYIVHVPYIFMTAWKMVYPFIDKKTKKKISFV--EDKK 275
            ++  R  + ILQ+ YPERLG  ++ + P +F   ++    F+D +T +K+ FV  +DK 
Sbjct: 161 PMKTTREIIHILQNYYPERLGIAFLYNPPRLFQAVYRAAKYFLDPRTAEKVKFVYPKDKA 220

Query: 276 LRSTLLSDIDESQLPDVYGGKLSL 281
               + +  D   LP  +GG+ +L
Sbjct: 221 SDELMTTHFDVENLPKEFGGEATL 241

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6597698.14.3e-13182.45Phosphatidylinositol transfer protein 3, partial [Cucurbita argyrosperma subsp. ... [more]
XP_023539819.14.3e-13182.45phosphatidylinositol transfer protein 3-like [Cucurbita pepo subsp. pepo][more]
KAG7029143.19.6e-13182.12Random slug protein 5 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022932698.11.4e-12982.06phosphatidylinositol transfer protein 3 [Cucurbita moschata][more]
XP_022972040.19.0e-12980.79phosphatidylinositol transfer protein 3-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q101371.3e-1631.16Sec14 cytosolic factor OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX... [more]
Q55CU81.1e-1526.33Phosphatidylinositol transfer protein 3 OS=Dictyostelium discoideum OX=44689 GN=... [more]
P333242.6e-1431.22CRAL-TRIO domain-containing protein YKL091C OS=Saccharomyces cerevisiae (strain ... [more]
P588752.9e-1328.50SEC14-like protein 2 OS=Bos taurus OX=9913 GN=SEC14L2 PE=1 SV=2[more]
O760542.9e-1326.17SEC14-like protein 2 OS=Homo sapiens OX=9606 GN=SEC14L2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1F2H36.7e-13082.06phosphatidylinositol transfer protein 3 OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1I7E94.3e-12980.79phosphatidylinositol transfer protein 3-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1DXU43.9e-12276.67random slug protein 5 OS=Momordica charantia OX=3673 GN=LOC111025140 PE=4 SV=1[more]
A0A6J1J0A31.5e-12175.58patellin-4-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111480094 PE=4 SV=1[more]
A0A6J1GUP11.3e-12075.16patellin-4-like OS=Cucurbita moschata OX=3662 GN=LOC111457645 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G01630.14.5e-8659.71Sec14p-like phosphatidylinositol transfer family protein [more]
AT1G14820.32.1e-3036.41Sec14p-like phosphatidylinositol transfer family protein [more]
AT1G14820.22.1e-3036.41Sec14p-like phosphatidylinositol transfer family protein [more]
AT1G14820.12.1e-3036.41Sec14p-like phosphatidylinositol transfer family protein [more]
AT4G36640.13.1e-1828.43Sec14p-like phosphatidylinositol transfer family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001251CRAL-TRIO lipid binding domainSMARTSM00516sec14_4coord: 141..279
e-value: 4.0E-41
score: 152.6
IPR001251CRAL-TRIO lipid binding domainPFAMPF00650CRAL_TRIOcoord: 165..277
e-value: 4.2E-31
score: 107.8
IPR001251CRAL-TRIO lipid binding domainPROSITEPS50191CRAL_TRIOcoord: 165..282
score: 19.873335
IPR001251CRAL-TRIO lipid binding domainCDDcd00170SEC14coord: 148..277
e-value: 2.1627E-40
score: 135.976
IPR011074CRAL/TRIO, N-terminal domainSMARTSM01100CRAL_TRIO_N_2coord: 95..120
e-value: 5.5E-5
score: 32.6
IPR036865CRAL-TRIO lipid binding domain superfamilyGENE3D3.40.525.10coord: 133..284
e-value: 4.9E-36
score: 126.1
IPR036865CRAL-TRIO lipid binding domain superfamilySUPERFAMILY52087CRAL/TRIO domaincoord: 137..279
NoneNo IPR availableGENE3D1.10.8.20coord: 10..126
e-value: 2.8E-11
score: 45.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..36
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 11..25
NoneNo IPR availablePANTHERPTHR46277:SF12BINDING PROTEIN, PUTATIVE-RELATEDcoord: 93..286
NoneNo IPR availablePANTHERPTHR46277OS03G0850700 PROTEINcoord: 44..73
NoneNo IPR availablePANTHERPTHR46277:SF12BINDING PROTEIN, PUTATIVE-RELATEDcoord: 44..73
NoneNo IPR availablePANTHERPTHR46277OS03G0850700 PROTEINcoord: 93..286
IPR036273CRAL/TRIO, N-terminal domain superfamilySUPERFAMILY46938CRAL/TRIO N-terminal domaincoord: 48..126

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg030945.1Spg030945.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane