Spg030668 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg030668
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionProtein NRT1/ PTR FAMILY 6.1
Locationscaffold11: 28434853 .. 28437697 (-)
RNA-Seq ExpressionSpg030668
SyntenySpg030668
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AACAGGTTTGGAGAAAAATTTTGTTTTGAATTCTTTAGTGAAAATAGTGAGTGGAAGTGGTTATATGAACATAGTGTTTGGTTTTGCTGTTGTGTTTTTTTTTTCCAGGAATTTAGAAGTATGGGAAGTAGAGAAATCAAATCACCTCAAGAGGTGATTGAAATGCCTGGAAGAGTGGATGAGCATTCAGAATCATTTCAGAGGAAGAAGCTTGGGATGTTTTTCGTCGAATCGGACGATCGGAGGACGGCGTTCGGGCGCGGTTACACCGGAGGAACGACTCCGGTTAATATCCGTGGCAAGCCCATTGCTGATCTTTCAAAGACTGGGGGCTGGATTGCAGCCTTCTTCATTTTTGGTAAGCAGCTGCAAAACAATCTTGTTGTTGTTTGTTGTTACTATTTGAACTTGGAAAGGAAAAAAGATGTAAAAGTTCACTCTTGTTTTGTTTACAAGGGAATGAAATGGCAGAGAGAATGGCTTATTTTGGGCTTTCAGTGAACATGGTAGCCTTCATGTTCTATGTGATGCATATGCCATTTACCACTTCATCAAATGCAGTTAACAATTTCCTTGGGATCTCACAAGCATCCTCTGTTCTCGGCGGTTTCCTCGCCGATGCATACCTCGGTCGATATTGGACGATTGCGATCTTCACTACCGTCTATCTCGGGGTACGATACCATCTTATAAATTCTATGAATACAAAGCTGATGAGGACCAAAGATTGAATTGAAATCCTTGGTTTGTACTTGATGCAGGGTTTAACAGGAATAACTCTATGTGCCACAATTAGTGCATTTGTGCCAAACCAACAGAAATGTGATCAGTTAACACTGCTTCTAGGCAAGTGTGAGCCAGCAAAGTCATGGCAAATGACTTACCTTTACGCAGTCCTTTATTTGACAGGGTTTGGGGCTGCAGGTATAAGGCCTTGTGTCTCTTCTTTTGGGGCTGATCAGTTTGATGAAAAGAGCAAGGACTACAAGTCTCACTTGGATCGCTTTTTCAACTTCTTTTACCTTTCTGTCACTATTGGAGCCATTGTGGCCTTCACTGCTGTTGTTTACATCCAAATGAAGCATGGATGGGGGGCTGCTTTTGGCTCCTTAGCCATAGCCATGGGCCTTTCCAACGTCGTCTTCTTTACCGGTACTCCTTTGTACCGGCATCGGTTGCCGGGAGGTAGCCCTCTCACAAGGGTTGCCCAAGTCCTTGTGGCTGCATTCAGGAAGAGACACACCTCGTTTTCGAGCAGCGAGATGGTTGGCTTGTATGAGGTTCCTGGCAAGCAATCCGCTATCAAGGGCAGTGGAAAGATCCTTCACACCGATGATTTCAGGTATGCGATACGATTCACAATTCTTTAACAATTTAACACAGTAAGGGGTCCTATATTCTAAACTTCTACGTCATTTCCTTTTCAACTAGTAACAGCCTATTCGTTGAATCTCAATAAATTTCGAACTTATAAATGAGGTGAAGGATTAACACAAAATTAAAAATATTAAATTTATCGTATTTTATCCCAGTAAGTTTTTAAGTTTAGTGGTGATTTGACCGTCGTTACACATGGATGGCAGATGTTTAGACAAGGCAGCACTACAGCTAAAGGAAGATGGAGGTAATCCAAGTCCATGGAGGCTATGCACAGTGACTCAAGTTGAGGAAGTGAAGATTCTTTTGAAGTTGATTCCAATACCAGCCTGCACAATCATGCTCAATTTGGTCCTAACAGAATATCTCACTCTTTCAGTGCAACAAGCATACACCATGAACACTCACATGGGCCGCCTTAAGCTCCCTGTCACATGCATGCCAGTCTTCCCTGGCCTTAGCATATTCCTCATACTTTCTCTCTACTACTCGGTATTCGTTCCGCTGTCTCGACGCATCACCGGACACCCCCACGGCGCTTCTCAGCTCCAGAGGGTCGGGATTGGTCTAGCGATCTCGATAATCTCGGTTGCCTGGGCCGGGGCATTCGAGAGATACCGAAGGAACTTTGCAATAAAAAATGGATATGAGGCCAGTTTTTTGACCCCTATGCCTAACTTGAGTGCCTTCTGGCTGCTCATTCAGTATTGTCTCATTGGCATAGCAGAAGTGTTCTGCATTGTTGGTTTGCTTGAGTTCTTATATGAGGAAGCCCCGGACGCCATGAAGAGCATTGGATCAGCATACGCCGCTCTTGCGGGCGGCTTGGGTTGCTTCGCGGCGTCGTTGTTAAACAGCATTATCAAATCCGTCACGGGGAACCCGAACGAAAGAATTCCATCTTGGCTTTCTCAAAACATTAACACGGGACGATTCGACTACTTCTACTGGCTTCTCACAGTCATGAGTATCATCAATTTCTGCATCTTTCTGTACTCAGCTCATAGGTACAAATACAGGAAAGATCATGAGGTTGGAGAAGGAATAATGGAAAATGGAAGGCATGATAAAATGTGAGATTCATCCACACTTTTCTTACTTTGATAGAGTGAACTACCATTAGAGTTGATTTTTAGAAATGTAAAAAAGAAATCAAAGCTACTTCTTCTACGGGAGTATTTGATTTCATAGTCGGTAAAAATTGAGAAATTATTCAATATAGCTGTAACACTTTCATATTCATTGCGGCCGAAAATTTCAATCTTCAATTCATACATCTACTAAGGTGAAAGTTGAAATGTGAATCACATTAAATAATTTCCTATCAAAGAATATATATTGTCATAATCATGGGCAGCTGTTGAATTAGAGCTAGCAGTGGAATTTAATGTTGGGACTCCTTTTGTCATTCTATTTACCAACTAAATGTAGTTGGCAATTATGATGAAATTAATTGCTGAGCAGTGAG

mRNA sequence

ATGGGAAGTAGAGAAATCAAATCACCTCAAGAGGTGATTGAAATGCCTGGAAGAGTGGATGAGCATTCAGAATCATTTCAGAGGAAGAAGCTTGGGATGTTTTTCGTCGAATCGGACGATCGGAGGACGGCGTTCGGGCGCGGTTACACCGGAGGAACGACTCCGGTTAATATCCGTGGCAAGCCCATTGCTGATCTTTCAAAGACTGGGGGCTGGATTGCAGCCTTCTTCATTTTTGGGAATGAAATGGCAGAGAGAATGGCTTATTTTGGGCTTTCAGTGAACATGGTAGCCTTCATGTTCTATGTGATGCATATGCCATTTACCACTTCATCAAATGCAGTTAACAATTTCCTTGGGATCTCACAAGCATCCTCTGTTCTCGGCGGTTTCCTCGCCGATGCATACCTCGGTCGATATTGGACGATTGCGATCTTCACTACCGTCTATCTCGGGGGTTTAACAGGAATAACTCTATGTGCCACAATTAGTGCATTTGTGCCAAACCAACAGAAATGTGATCAGTTAACACTGCTTCTAGGCAAGTGTGAGCCAGCAAAGTCATGGCAAATGACTTACCTTTACGCAGTCCTTTATTTGACAGGGTTTGGGGCTGCAGGTATAAGGCCTTGTGTCTCTTCTTTTGGGGCTGATCAGTTTGATGAAAAGAGCAAGGACTACAAGTCTCACTTGGATCGCTTTTTCAACTTCTTTTACCTTTCTGTCACTATTGGAGCCATTGTGGCCTTCACTGCTGTTGTTTACATCCAAATGAAGCATGGATGGGGGGCTGCTTTTGGCTCCTTAGCCATAGCCATGGGCCTTTCCAACGTCGTCTTCTTTACCGGTACTCCTTTGTACCGGCATCGGTTGCCGGGAGGTAGCCCTCTCACAAGGGTTGCCCAAGTCCTTGTGGCTGCATTCAGGAAGAGACACACCTCGTTTTCGAGCAGCGAGATGGTTGGCTTGTATGAGGTTCCTGGCAAGCAATCCGCTATCAAGGGCAGTGGAAAGATCCTTCACACCGATGATTTCAGATGTTTAGACAAGGCAGCACTACAGCTAAAGGAAGATGGAGGTAATCCAAGTCCATGGAGGCTATGCACAGTGACTCAAGTTGAGGAAGTGAAGATTCTTTTGAAGTTGATTCCAATACCAGCCTGCACAATCATGCTCAATTTGGTCCTAACAGAATATCTCACTCTTTCAGTGCAACAAGCATACACCATGAACACTCACATGGGCCGCCTTAAGCTCCCTGTCACATGCATGCCAGTCTTCCCTGGCCTTAGCATATTCCTCATACTTTCTCTCTACTACTCGGTATTCGTTCCGCTGTCTCGACGCATCACCGGACACCCCCACGGCGCTTCTCAGCTCCAGAGGGTCGGGATTGGTCTAGCGATCTCGATAATCTCGGTTGCCTGGGCCGGGGCATTCGAGAGATACCGAAGGAACTTTGCAATAAAAAATGGATATGAGGCCAGTTTTTTGACCCCTATGCCTAACTTGAGTGCCTTCTGGCTGCTCATTCAGTATTGTCTCATTGGCATAGCAGAAGTGTTCTGCATTGTTGGTTTGCTTGAGTTCTTATATGAGGAAGCCCCGGACGCCATGAAGAGCATTGGATCAGCATACGCCGCTCTTGCGGGCGGCTTGGGTTGCTTCGCGGCGTCGTTGTTAAACAGCATTATCAAATCCGTCACGGGGAACCCGAACGAAAGAATTCCATCTTGGCTTTCTCAAAACATTAACACGGGACGATTCGACTACTTCTACTGGCTTCTCACAGTCATGAGTATCATCAATTTCTGCATCTTTCTGTACTCAGCTCATAGGTACAAATACAGGAAAGATCATGAGGTTGGAGAAGGAATAATGGAAAATGGAAGGCATGATAAAATGTGA

Coding sequence (CDS)

ATGGGAAGTAGAGAAATCAAATCACCTCAAGAGGTGATTGAAATGCCTGGAAGAGTGGATGAGCATTCAGAATCATTTCAGAGGAAGAAGCTTGGGATGTTTTTCGTCGAATCGGACGATCGGAGGACGGCGTTCGGGCGCGGTTACACCGGAGGAACGACTCCGGTTAATATCCGTGGCAAGCCCATTGCTGATCTTTCAAAGACTGGGGGCTGGATTGCAGCCTTCTTCATTTTTGGGAATGAAATGGCAGAGAGAATGGCTTATTTTGGGCTTTCAGTGAACATGGTAGCCTTCATGTTCTATGTGATGCATATGCCATTTACCACTTCATCAAATGCAGTTAACAATTTCCTTGGGATCTCACAAGCATCCTCTGTTCTCGGCGGTTTCCTCGCCGATGCATACCTCGGTCGATATTGGACGATTGCGATCTTCACTACCGTCTATCTCGGGGGTTTAACAGGAATAACTCTATGTGCCACAATTAGTGCATTTGTGCCAAACCAACAGAAATGTGATCAGTTAACACTGCTTCTAGGCAAGTGTGAGCCAGCAAAGTCATGGCAAATGACTTACCTTTACGCAGTCCTTTATTTGACAGGGTTTGGGGCTGCAGGTATAAGGCCTTGTGTCTCTTCTTTTGGGGCTGATCAGTTTGATGAAAAGAGCAAGGACTACAAGTCTCACTTGGATCGCTTTTTCAACTTCTTTTACCTTTCTGTCACTATTGGAGCCATTGTGGCCTTCACTGCTGTTGTTTACATCCAAATGAAGCATGGATGGGGGGCTGCTTTTGGCTCCTTAGCCATAGCCATGGGCCTTTCCAACGTCGTCTTCTTTACCGGTACTCCTTTGTACCGGCATCGGTTGCCGGGAGGTAGCCCTCTCACAAGGGTTGCCCAAGTCCTTGTGGCTGCATTCAGGAAGAGACACACCTCGTTTTCGAGCAGCGAGATGGTTGGCTTGTATGAGGTTCCTGGCAAGCAATCCGCTATCAAGGGCAGTGGAAAGATCCTTCACACCGATGATTTCAGATGTTTAGACAAGGCAGCACTACAGCTAAAGGAAGATGGAGGTAATCCAAGTCCATGGAGGCTATGCACAGTGACTCAAGTTGAGGAAGTGAAGATTCTTTTGAAGTTGATTCCAATACCAGCCTGCACAATCATGCTCAATTTGGTCCTAACAGAATATCTCACTCTTTCAGTGCAACAAGCATACACCATGAACACTCACATGGGCCGCCTTAAGCTCCCTGTCACATGCATGCCAGTCTTCCCTGGCCTTAGCATATTCCTCATACTTTCTCTCTACTACTCGGTATTCGTTCCGCTGTCTCGACGCATCACCGGACACCCCCACGGCGCTTCTCAGCTCCAGAGGGTCGGGATTGGTCTAGCGATCTCGATAATCTCGGTTGCCTGGGCCGGGGCATTCGAGAGATACCGAAGGAACTTTGCAATAAAAAATGGATATGAGGCCAGTTTTTTGACCCCTATGCCTAACTTGAGTGCCTTCTGGCTGCTCATTCAGTATTGTCTCATTGGCATAGCAGAAGTGTTCTGCATTGTTGGTTTGCTTGAGTTCTTATATGAGGAAGCCCCGGACGCCATGAAGAGCATTGGATCAGCATACGCCGCTCTTGCGGGCGGCTTGGGTTGCTTCGCGGCGTCGTTGTTAAACAGCATTATCAAATCCGTCACGGGGAACCCGAACGAAAGAATTCCATCTTGGCTTTCTCAAAACATTAACACGGGACGATTCGACTACTTCTACTGGCTTCTCACAGTCATGAGTATCATCAATTTCTGCATCTTTCTGTACTCAGCTCATAGGTACAAATACAGGAAAGATCATGAGGTTGGAGAAGGAATAATGGAAAATGGAAGGCATGATAAAATGTGA

Protein sequence

MGSREIKSPQEVIEMPGRVDEHSESFQRKKLGMFFVESDDRRTAFGRGYTGGTTPVNIRGKPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSNAVNNFLGISQASSVLGGFLADAYLGRYWTIAIFTTVYLGGLTGITLCATISAFVPNQQKCDQLTLLLGKCEPAKSWQMTYLYAVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYLSVTIGAIVAFTAVVYIQMKHGWGAAFGSLAIAMGLSNVVFFTGTPLYRHRLPGGSPLTRVAQVLVAAFRKRHTSFSSSEMVGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQLKEDGGNPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTHMGRLKLPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAGAFERYRRNFAIKNGYEASFLTPMPNLSAFWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMKSIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQNINTGRFDYFYWLLTVMSIINFCIFLYSAHRYKYRKDHEVGEGIMENGRHDKM
Homology
BLAST of Spg030668 vs. NCBI nr
Match: XP_038903063.1 (protein NRT1/ PTR FAMILY 6.1 isoform X1 [Benincasa hispida])

HSP 1 Score: 1214.1 bits (3140), Expect = 0.0e+00
Identity = 603/637 (94.66%), Postives = 622/637 (97.65%), Query Frame = 0

Query: 1   MGSREIKSPQEVIEMP--GRVDEHSESFQRKKLGMFFVESDDRRTAFGRGYTGGTTPVNI 60
           MGSREIKSPQEVIE    GR+DEHSESFQRKKLGMFF+ESDDRRTAFGRGYTGGTTPVNI
Sbjct: 1   MGSREIKSPQEVIETSGLGRLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNI 60

Query: 61  RGKPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSNAVNNF 120
           RGKPIADLSKTGGW+AAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSS+AVNNF
Sbjct: 61  RGKPIADLSKTGGWVAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNF 120

Query: 121 LGISQASSVLGGFLADAYLGRYWTIAIFTTVYLGGLTGITLCATISAFVPNQQKCDQLTL 180
           LGISQASSVLGGFLADAYLGRYWTIAIFTT+YLGGLTGITLCATIS FVPNQ+KCDQL+L
Sbjct: 121 LGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLTGITLCATISTFVPNQEKCDQLSL 180

Query: 181 LLGKCEPAKSWQMTYLYAVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF 240
           LLGKCEPAKSWQMTYLY VLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF
Sbjct: 181 LLGKCEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF 240

Query: 241 YLSVTIGAIVAFTAVVYIQMKHGWGAAFGSLAIAMGLSNVVFFTGTPLYRHRLPGGSPLT 300
           YLSVT+GAI+AFTAVVYIQ+KHGWGAAFGSLAIAMG SNVVFF GTPLYRHRLPGGSPLT
Sbjct: 241 YLSVTVGAIIAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLT 300

Query: 301 RVAQVLVAAFRKRHTSFSSSEMVGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQLKED 360
           RVAQVLVAA+RKR+ SFS+SE VGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQLKED
Sbjct: 301 RVAQVLVAAYRKRNISFSNSEFVGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQLKED 360

Query: 361 GGNPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTHMGRLK 420
           G NPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTH+G LK
Sbjct: 361 GRNPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTHIGHLK 420

Query: 421 LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAG 480
           LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISI+SVAW G
Sbjct: 421 LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIMSVAWGG 480

Query: 481 AFERYRRNFAIKNGYEASFLTPMPNLSAFWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA 540
           AFERYRRN+AI+NGYEASFLTPMPNLSA+WLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA
Sbjct: 481 AFERYRRNYAIENGYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA 540

Query: 541 MKSIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQNINTGRFDYFYWLLTV 600
           MKSIGSAYAALAGGLGCFAASLLNSIIKS+TGNPNER PSWLSQNINTGRFDYFYWLLTV
Sbjct: 541 MKSIGSAYAALAGGLGCFAASLLNSIIKSITGNPNERNPSWLSQNINTGRFDYFYWLLTV 600

Query: 601 MSIINFCIFLYSAHRYKYRKDHEVGEGIMENGRHDKM 636
           MS INFCIFLYSAHRYKYRKDHEVGEGIMENGRHDKM
Sbjct: 601 MSFINFCIFLYSAHRYKYRKDHEVGEGIMENGRHDKM 637

BLAST of Spg030668 vs. NCBI nr
Match: XP_011653004.1 (protein NRT1/ PTR FAMILY 6.1 [Cucumis sativus] >XP_031740724.1 protein NRT1/ PTR FAMILY 6.1 [Cucumis sativus] >KGN52956.1 hypothetical protein Csa_014689 [Cucumis sativus])

HSP 1 Score: 1199.5 bits (3102), Expect = 0.0e+00
Identity = 597/637 (93.72%), Postives = 619/637 (97.17%), Query Frame = 0

Query: 1   MGSREIKSPQEVIEMP--GRVDEHSESFQRKKLGMFFVESDDRRTAFGRGYTGGTTPVNI 60
           MGSREIKS  EV E P  GR+DEHSESFQRKKLGMFF+ESDDRRTAFGRGYTGGTTPVNI
Sbjct: 1   MGSREIKSLHEVNETPVLGRLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNI 60

Query: 61  RGKPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSNAVNNF 120
           RGKPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSS+AVNNF
Sbjct: 61  RGKPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNF 120

Query: 121 LGISQASSVLGGFLADAYLGRYWTIAIFTTVYLGGLTGITLCATISAFVPNQQKCDQLTL 180
           LGISQASSVLGGFLADAYLGRYWTIAIFTT+YLGGL GITLCATIS FVPNQ KCDQL+L
Sbjct: 121 LGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLVGITLCATISTFVPNQAKCDQLSL 180

Query: 181 LLGKCEPAKSWQMTYLYAVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF 240
           LLG+CEPAK WQMTYLY VLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF
Sbjct: 181 LLGRCEPAKPWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF 240

Query: 241 YLSVTIGAIVAFTAVVYIQMKHGWGAAFGSLAIAMGLSNVVFFTGTPLYRHRLPGGSPLT 300
           YLSVT+GAI+AFTAVVYIQ+KHGWGAAFGSLAIAMG SNVVFF GTPLYRHRLPGGSPLT
Sbjct: 241 YLSVTVGAIIAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLT 300

Query: 301 RVAQVLVAAFRKRHTSFSSSEMVGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQLKED 360
           RVAQVLVAA+RKR+ SFS+SE VGL+EVPGKQSAIKGSGKILHTDDFRCLDKAALQLKED
Sbjct: 301 RVAQVLVAAYRKRNASFSNSEFVGLFEVPGKQSAIKGSGKILHTDDFRCLDKAALQLKED 360

Query: 361 GGNPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTHMGRLK 420
           GGNPSPW+LCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTH+GRLK
Sbjct: 361 GGNPSPWKLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTHIGRLK 420

Query: 421 LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAG 480
           LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISI+SVAWAG
Sbjct: 421 LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISILSVAWAG 480

Query: 481 AFERYRRNFAIKNGYEASFLTPMPNLSAFWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA 540
           AFERYRRNFAI++GYEASFL+PMPNLSA+WLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA
Sbjct: 481 AFERYRRNFAIRSGYEASFLSPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA 540

Query: 541 MKSIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQNINTGRFDYFYWLLTV 600
           MKSIGSAYAALAGGLGCFAASLLNSIIKSVTG+PN R PSWLSQNINTGRFDYFYWLLTV
Sbjct: 541 MKSIGSAYAALAGGLGCFAASLLNSIIKSVTGSPNGRNPSWLSQNINTGRFDYFYWLLTV 600

Query: 601 MSIINFCIFLYSAHRYKYRKDHEVGEGIMENGRHDKM 636
           MSIINFCIFLYSAH+YKYRKDHEVGEGIMENG HDKM
Sbjct: 601 MSIINFCIFLYSAHKYKYRKDHEVGEGIMENGMHDKM 637

BLAST of Spg030668 vs. NCBI nr
Match: KAA0044494.1 (protein NRT1/ PTR FAMILY 6.1 [Cucumis melo var. makuwa])

HSP 1 Score: 1198.0 bits (3098), Expect = 0.0e+00
Identity = 596/637 (93.56%), Postives = 619/637 (97.17%), Query Frame = 0

Query: 1   MGSREIKSPQEVIEMP--GRVDEHSESFQRKKLGMFFVESDDRRTAFGRGYTGGTTPVNI 60
           MGSREIKS QEV E P  GR+DEHSESFQRKKLGMFF+ESDDRRTAFGRGYTGGTTPVNI
Sbjct: 1   MGSREIKSLQEVNETPVLGRLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNI 60

Query: 61  RGKPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSNAVNNF 120
           RGKPI DLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFT+SS+AVNNF
Sbjct: 61  RGKPIVDLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTSSSDAVNNF 120

Query: 121 LGISQASSVLGGFLADAYLGRYWTIAIFTTVYLGGLTGITLCATISAFVPNQQKCDQLTL 180
           LGISQASSVLGGFLADAYLGRYWTIAIFTT+YLGGL GITLCATIS FVPNQ KCDQL+L
Sbjct: 121 LGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLVGITLCATISTFVPNQAKCDQLSL 180

Query: 181 LLGKCEPAKSWQMTYLYAVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF 240
           LLG+CEPAKSWQMTYLY VLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF
Sbjct: 181 LLGRCEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF 240

Query: 241 YLSVTIGAIVAFTAVVYIQMKHGWGAAFGSLAIAMGLSNVVFFTGTPLYRHRLPGGSPLT 300
           YLSVT+GAI+AFTAVVYIQ++HGWGAAFGSLAIAMG SNVVFF GTPLYRHRLPGGSPLT
Sbjct: 241 YLSVTVGAIIAFTAVVYIQIQHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLT 300

Query: 301 RVAQVLVAAFRKRHTSFSSSEMVGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQLKED 360
           RVAQVLVAA+RKR+ SFS+SE VGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQLKED
Sbjct: 301 RVAQVLVAAYRKRNASFSNSEFVGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQLKED 360

Query: 361 GGNPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTHMGRLK 420
           GGNPSPW+LCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTH+GRLK
Sbjct: 361 GGNPSPWKLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTHIGRLK 420

Query: 421 LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAG 480
           LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISI+SVAWAG
Sbjct: 421 LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISILSVAWAG 480

Query: 481 AFERYRRNFAIKNGYEASFLTPMPNLSAFWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA 540
           AFERYRRNFA+++GYEASFL+PMPNLSA+WLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA
Sbjct: 481 AFERYRRNFAVRSGYEASFLSPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA 540

Query: 541 MKSIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQNINTGRFDYFYWLLTV 600
           MKSIGSAYAALAGGLGCFAASLLNSIIKSVTG+ N R PSWLSQNINTGRFDYFYWLLTV
Sbjct: 541 MKSIGSAYAALAGGLGCFAASLLNSIIKSVTGSTNGRNPSWLSQNINTGRFDYFYWLLTV 600

Query: 601 MSIINFCIFLYSAHRYKYRKDHEVGEGIMENGRHDKM 636
           MSIINFCIFLYSAHRYKYRKDHEVGEGIMENG HDKM
Sbjct: 601 MSIINFCIFLYSAHRYKYRKDHEVGEGIMENGMHDKM 637

BLAST of Spg030668 vs. NCBI nr
Match: XP_008454154.1 (PREDICTED: protein NRT1/ PTR FAMILY 6.1 [Cucumis melo] >XP_008454155.1 PREDICTED: protein NRT1/ PTR FAMILY 6.1 [Cucumis melo] >XP_008454156.1 PREDICTED: protein NRT1/ PTR FAMILY 6.1 [Cucumis melo] >TYK29622.1 protein NRT1/ PTR FAMILY 6.1 [Cucumis melo var. makuwa])

HSP 1 Score: 1197.6 bits (3097), Expect = 0.0e+00
Identity = 596/637 (93.56%), Postives = 619/637 (97.17%), Query Frame = 0

Query: 1   MGSREIKSPQEVIEMP--GRVDEHSESFQRKKLGMFFVESDDRRTAFGRGYTGGTTPVNI 60
           MGSREIKS QEV E P  GR+DEHSESFQRKKLGMFF+ESDDRRTAFGRGYTGGTTPVNI
Sbjct: 1   MGSREIKSLQEVNETPVLGRLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNI 60

Query: 61  RGKPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSNAVNNF 120
           RGKPI DLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFT+SS+AVNNF
Sbjct: 61  RGKPIVDLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTSSSDAVNNF 120

Query: 121 LGISQASSVLGGFLADAYLGRYWTIAIFTTVYLGGLTGITLCATISAFVPNQQKCDQLTL 180
           LGISQASSVLGGFLADAYLGRYWTIAIFTT+YLGGL GITLCATIS FVPNQ KCDQL+L
Sbjct: 121 LGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLVGITLCATISRFVPNQAKCDQLSL 180

Query: 181 LLGKCEPAKSWQMTYLYAVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF 240
           LLG+CEPAKSWQMTYLY VLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF
Sbjct: 181 LLGRCEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF 240

Query: 241 YLSVTIGAIVAFTAVVYIQMKHGWGAAFGSLAIAMGLSNVVFFTGTPLYRHRLPGGSPLT 300
           YLSVT+GAI+AFTAVVYIQ++HGWGAAFGSLAIAMG SNVVFF GTPLYRHRLPGGSPLT
Sbjct: 241 YLSVTVGAIIAFTAVVYIQIQHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLT 300

Query: 301 RVAQVLVAAFRKRHTSFSSSEMVGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQLKED 360
           RVAQVLVAA+RKR+ SFS+SE VGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQLKED
Sbjct: 301 RVAQVLVAAYRKRNASFSNSEFVGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQLKED 360

Query: 361 GGNPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTHMGRLK 420
           GGNPSPW+LCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTH+GRLK
Sbjct: 361 GGNPSPWKLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTHIGRLK 420

Query: 421 LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAG 480
           LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISI+SVAWAG
Sbjct: 421 LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISILSVAWAG 480

Query: 481 AFERYRRNFAIKNGYEASFLTPMPNLSAFWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA 540
           AFERYRRNFA+++GYEASFL+PMPNLSA+WLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA
Sbjct: 481 AFERYRRNFAVRSGYEASFLSPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA 540

Query: 541 MKSIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQNINTGRFDYFYWLLTV 600
           MKSIGSAYAALAGGLGCFAASLLNSIIKSVTG+ N R PSWLSQNINTGRFDYFYWLLTV
Sbjct: 541 MKSIGSAYAALAGGLGCFAASLLNSIIKSVTGSTNGRNPSWLSQNINTGRFDYFYWLLTV 600

Query: 601 MSIINFCIFLYSAHRYKYRKDHEVGEGIMENGRHDKM 636
           MSIINFCIFLYSAHRYKYRKDHEVGEGIMENG HDKM
Sbjct: 601 MSIINFCIFLYSAHRYKYRKDHEVGEGIMENGMHDKM 637

BLAST of Spg030668 vs. NCBI nr
Match: XP_022972287.1 (protein NRT1/ PTR FAMILY 6.1 [Cucurbita maxima])

HSP 1 Score: 1182.5 bits (3058), Expect = 0.0e+00
Identity = 590/635 (92.91%), Postives = 612/635 (96.38%), Query Frame = 0

Query: 1   MGSREIKSPQEVIEMPGRVDEHSESFQRKKLGMFFVESDDRRTAFGRGYTGGTTPVNIRG 60
           MGS EIKSPQE++EMP R+DE     QRKKLGMFFVESDDRRTAFGRGYTGGTTPVNIRG
Sbjct: 1   MGSTEIKSPQEMVEMPARLDE-----QRKKLGMFFVESDDRRTAFGRGYTGGTTPVNIRG 60

Query: 61  KPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSNAVNNFLG 120
           KPIADLSKTGGW AAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSS+AVNNFLG
Sbjct: 61  KPIADLSKTGGWTAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNFLG 120

Query: 121 ISQASSVLGGFLADAYLGRYWTIAIFTTVYLGGLTGITLCATISAFVPNQQKCDQLTLLL 180
           ISQASSVLGGF+ADAYLGRYWTIAIFTT+YLGGLTGITLCATISAFVPNQ+ CD+L+LLL
Sbjct: 121 ISQASSVLGGFIADAYLGRYWTIAIFTTIYLGGLTGITLCATISAFVPNQKICDELSLLL 180

Query: 181 GKCEPAKSWQMTYLYAVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYL 240
           GKCEPAK+WQMTYLY VLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYL
Sbjct: 181 GKCEPAKAWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYL 240

Query: 241 SVTIGAIVAFTAVVYIQMKHGWGAAFGSLAIAMGLSNVVFFTGTPLYRHRLPGGSPLTRV 300
           SVT GAIVAFTAVVYIQ+KHGWGAAFGSLAIAMG SNVVFF GTPLYRHRLPGGSPLTRV
Sbjct: 241 SVTTGAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLTRV 300

Query: 301 AQVLVAAFRKRHTSFSSSEMVGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQLKEDGG 360
           AQVLVAAFRKR+ SFSSSEMVGLYEV GK SAIKGSGKILHT+DFRCLDKAAL+LK+DGG
Sbjct: 301 AQVLVAAFRKRNASFSSSEMVGLYEVAGKHSAIKGSGKILHTNDFRCLDKAALELKDDGG 360

Query: 361 NPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTHMGRLKLP 420
           NPSPWRLCTVTQVEEVKILLKLIPIPACTI+LNLVLTEYLTLSVQQAYT+NTHMGRLKLP
Sbjct: 361 NPSPWRLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTLSVQQAYTLNTHMGRLKLP 420

Query: 421 VTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAGAF 480
           VTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAG F
Sbjct: 421 VTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAGVF 480

Query: 481 ERYRRNFAIKNGYEASFLTPMPNLSAFWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMK 540
           ERYRRN+AIK+GYEASFLTPMPNLSA+WLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMK
Sbjct: 481 ERYRRNYAIKSGYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMK 540

Query: 541 SIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQNINTGRFDYFYWLLTVMS 600
           SIGS+YAALAGGLGCFAASLLNSIIKSVTGNP E  PSWLSQNIN G+FDYFYWLLTVMS
Sbjct: 541 SIGSSYAALAGGLGCFAASLLNSIIKSVTGNPKEGTPSWLSQNINKGQFDYFYWLLTVMS 600

Query: 601 IINFCIFLYSAHRYKYRKDHEVGEGIMENGRHDKM 636
           IINFCIFLYSAHRYKYRKDHEVGEGIMENGRH  M
Sbjct: 601 IINFCIFLYSAHRYKYRKDHEVGEGIMENGRHGTM 630

BLAST of Spg030668 vs. ExPASy Swiss-Prot
Match: Q9LYR6 (Protein NRT1/ PTR FAMILY 6.1 OS=Arabidopsis thaliana OX=3702 GN=NPF6.1 PE=2 SV=1)

HSP 1 Score: 988.4 bits (2554), Expect = 3.7e-287
Identity = 492/617 (79.74%), Postives = 553/617 (89.63%), Query Frame = 0

Query: 1   MGSREIKSPQEVIEMPGRVDEHSESFQRKKLGMFFVESDDRRTAFGRGYTGGTTPVNIRG 60
           M + EIKSP  V E PG    H     RK+L +FF+ESD+RR A GRGYTGGTTPVNI G
Sbjct: 1   MVASEIKSPVSVPETPGSSSVH----HRKQLSVFFIESDNRRLALGRGYTGGTTPVNIHG 60

Query: 61  KPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSNAVNNFLG 120
           KPIA+LSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMH PF +SSNAVNNFLG
Sbjct: 61  KPIANLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHRPFESSSNAVNNFLG 120

Query: 121 ISQASSVLGGFLADAYLGRYWTIAIFTTVYLGGLTGITLCATISAFVPNQQKCDQLTLLL 180
           ISQASSVLGGFLADAYLGRYWTIAIFTT+YL GL GITL A++  FVP+Q  C QL+LLL
Sbjct: 121 ISQASSVLGGFLADAYLGRYWTIAIFTTMYLVGLIGITLGASLKMFVPDQSNCGQLSLLL 180

Query: 181 GKCEPAKSWQMTYLYAVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYL 240
           G CE AKSWQM YLY VLY+TGFGAAGIRPCVSSFGADQFDEKSKDYK+HLDRFFNFFYL
Sbjct: 181 GNCEEAKSWQMLYLYTVLYITGFGAAGIRPCVSSFGADQFDEKSKDYKTHLDRFFNFFYL 240

Query: 241 SVTIGAIVAFTAVVYIQMKHGWGAAFGSLAIAMGLSNVVFFTGTPLYRHRLPGGSPLTRV 300
           SVT+GAI+AFT VVY+QM+ GWG AFG+LA+AMG+SN +FF GTPLYRHRLPGGSPLTRV
Sbjct: 241 SVTLGAIIAFTLVVYVQMELGWGMAFGTLAVAMGISNALFFAGTPLYRHRLPGGSPLTRV 300

Query: 301 AQVLVAAFRKRHTSFSSSEMVGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQLKEDGG 360
           AQVLVAAFRKR+ +F+SSE +GLYEVPG +SAI GS KI H++DF  LDKAAL+LKEDG 
Sbjct: 301 AQVLVAAFRKRNAAFTSSEFIGLYEVPGLKSAINGSRKIPHSNDFIWLDKAALELKEDGL 360

Query: 361 NPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTHMGRLKLP 420
            PSPW+LCTVTQVEEVKIL++LIPIP CTIML+LVLTEYLTLSVQQAYT+NTH+  LKLP
Sbjct: 361 EPSPWKLCTVTQVEEVKILIRLIPIPTCTIMLSLVLTEYLTLSVQQAYTLNTHIQHLKLP 420

Query: 421 VTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAGAF 480
           VTCMPVFPGLSIFLILSLYYSVFVP++RRITG+PHGASQLQRVGIGLA+SIISVAWAG F
Sbjct: 421 VTCMPVFPGLSIFLILSLYYSVFVPITRRITGNPHGASQLQRVGIGLAVSIISVAWAGLF 480

Query: 481 ERYRRNFAIKNGYEASFLTPMPNLSAFWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMK 540
           E YRR++AI+NG+E +FLT MP+L+A+WLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMK
Sbjct: 481 ENYRRHYAIQNGFEFNFLTQMPDLTAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMK 540

Query: 541 SIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQNINTGRFDYFYWLLTVMS 600
           SIGSAYAALAGGLGCFAA++LN+I+K+ T + + +  SWLSQNINTGRFD  YWLLT++S
Sbjct: 541 SIGSAYAALAGGLGCFAATILNNIVKAATRDSDGK--SWLSQNINTGRFDCLYWLLTLLS 600

Query: 601 IINFCIFLYSAHRYKYR 618
            +NFC+FL+SAHRYKYR
Sbjct: 601 FLNFCVFLWSAHRYKYR 611

BLAST of Spg030668 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 1.8e-116
Identity = 229/568 (40.32%), Postives = 344/568 (60.56%), Query Frame = 0

Query: 56  VNIRGKPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSNAV 115
           V+I   P A+  KTG W A  FI GNE  ER+AY+G+  N+V ++   ++    T++N V
Sbjct: 13  VDIHKNP-ANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNATAANNV 72

Query: 116 NNFLGISQASSVLGGFLADAYLGRYWTIAIFTTVYLGGLTGITLCATISAFVPNQQKCDQ 175
            N+ G    + ++G F+ADAYLGRYWTIA F  +Y+ G+T +TL A++    P     D 
Sbjct: 73  TNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGNCNAD- 132

Query: 176 LTLLLGKCEPAKSWQMTYLYAVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFF 235
                  C P  S Q    +  LY+   G  GI+PCVSSFGADQFDE  ++ K     FF
Sbjct: 133 ------TCHPNSS-QTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFF 192

Query: 236 NFFYLSVTIGAIVAFTAVVYIQMKHGWGAAFGSLAIAMGLSNVVFFTGTPLYRHRLPGGS 295
           N+FY S+ +GA++A T +V+IQM  GWG  FG   +AM ++   FF G+  YR + PGGS
Sbjct: 193 NWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGS 252

Query: 296 PLTRVAQVLVAAFRKRHTSFSSSEMVGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQL 355
           PLTR+ QV+VAAFRK        + + L+E    +S IKGS K++HTD+ +  DKAA++ 
Sbjct: 253 PLTRIFQVIVAAFRKISVKVPEDKSL-LFETADDESNIKGSRKLVHTDNLKFFDKAAVES 312

Query: 356 KEDG---GNPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNT 415
           + D    G  +PWRLC+VTQVEE+K ++ L+P+ A  I+   V ++  T+ V Q  TM+ 
Sbjct: 313 QSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQ 372

Query: 416 HMGR-LKLPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISI 475
           HMG+  ++P   + +F  +S+     +Y    +PL+R+ T +  G +QLQR+GIGL +SI
Sbjct: 373 HMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVVSI 432

Query: 476 ISVAWAGAFERYRRNFA-IKNGYEASFLTPMPNLSAFWLLIQYCLIGIAEVFCIVGLLEF 535
            ++  AG  E  R ++    N Y+   +    ++S FW + QY LIG AEVF  +G LEF
Sbjct: 433 FAMITAGVLEVVRLDYVKTHNAYDQKQI----HMSIFWQIPQYLLIGCAEVFTFIGQLEF 492

Query: 536 LYEEAPDAMKSIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQNINTGRFD 595
            Y++APDAM+S+ SA +     LG + +++L +++  +T    +  P W+  N+N G  D
Sbjct: 493 FYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGK--PGWIPDNLNRGHLD 552

Query: 596 YFYWLLTVMSIINFCIFLYSAHRYKYRK 619
           YF++LL  +S +NF ++L+ + RYKY+K
Sbjct: 553 YFFYLLATLSFLNFLVYLWISKRYKYKK 564

BLAST of Spg030668 vs. ExPASy Swiss-Prot
Match: P46032 (Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 3.8e-114
Identity = 232/566 (40.99%), Postives = 336/566 (59.36%), Query Frame = 0

Query: 56  VNIRGKPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSNAV 115
           V+  G P     KTG W A  FI GNE  ER+AY+G++ N++ ++   +H    +++  V
Sbjct: 30  VDFNGNPPLK-EKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLHQGNVSAATNV 89

Query: 116 NNFLGISQASSVLGGFLADAYLGRYWTIAIFTTVYLGGLTGITLCATISAFVPNQQKCDQ 175
             + G    + ++G  LADAY GRYWTIA F+ +Y  G++ +TL A++ A  P +   D 
Sbjct: 90  TTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKPAECIGD- 149

Query: 176 LTLLLGKCEPAKSWQMTYLYAVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFF 235
                  C  A   Q    +  LYL   G  GI+PCVSSFGADQFD+     +     FF
Sbjct: 150 ------FCPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKASFF 209

Query: 236 NFFYLSVTIGAIVAFTAVVYIQMKHGWGAAFGSLAIAMGLSNVVFFTGTPLYRHRLPGGS 295
           N+FY S+ IGA+V+ + +V+IQ   GWG  FG   + MGL+   FF GTPLYR + PGGS
Sbjct: 210 NWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKPGGS 269

Query: 296 PLTRVAQVLVAAFRKRHTSFSSSEMVGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQL 355
           P+TR++QV+VA+FRK          + LYE   K SAI GS KI HTDD + LDKAA+  
Sbjct: 270 PITRISQVVVASFRKSSVKVPEDATL-LYETQDKNSAIAGSRKIEHTDDCQYLDKAAVIS 329

Query: 356 KED---GGNPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNT 415
           +E+   G   + WRLCTVTQVEE+KIL+++ PI A  I+ + V  +  T+ VQQ   MN 
Sbjct: 330 EEESKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGRAMNC 389

Query: 416 HMGRLKLPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISII 475
            +G  +LP   +  F   S+ + + LY    VPL+R+ TG   G +++QR+GIGL +S++
Sbjct: 390 KIGSFQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGLFVSVL 449

Query: 476 SVAWAGAFERYRRNFAIKNGYEASFLTPMPNLSAFWLLIQYCLIGIAEVFCIVGLLEFLY 535
            +A A   E  R + A   G   S   P+P +S  W + QY ++G AEVF  +G LEF Y
Sbjct: 450 CMAAAAIVEIIRLHMANDLGLVESG-APVP-ISVLWQIPQYFILGAAEVFYFIGQLEFFY 509

Query: 536 EEAPDAMKSIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQNINTGRFDYF 595
           +++PDAM+S+ SA A L   LG + +SL+ +++   T    +    W+S N+N+G  DYF
Sbjct: 510 DQSPDAMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNGQE--GWISDNLNSGHLDYF 569

Query: 596 YWLLTVMSIINFCIFLYSAHRYKYRK 619
           +WLL  +S++N  ++ +SA RYK +K
Sbjct: 570 FWLLAGLSLVNMAVYFFSAARYKQKK 582

BLAST of Spg030668 vs. ExPASy Swiss-Prot
Match: Q9LFB8 (Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 3.2e-113
Identity = 230/567 (40.56%), Postives = 338/567 (59.61%), Query Frame = 0

Query: 56  VNIRGKPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSNAV 115
           ++I  KP A+ +KTG W A  FI G E  ER+AY+G+S N++ ++   M+M   ++S +V
Sbjct: 14  LDIHKKP-ANKNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQMNMENVSASKSV 73

Query: 116 NNFLGISQASSVLGGFLADAYLGRYWTIAIFTTVYLGGLTGITLCATISAFVPNQQKCDQ 175
           +N+ G   A+ ++G F+ADAYLGRYWTIA F  +Y+ G+T +T+ A++    P    C  
Sbjct: 74  SNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVPGLTPT---CS- 133

Query: 176 LTLLLGKCEPAKSWQMTYLYAVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFF 235
                G+   A + Q    +  LYL   G  GI+PCVSSFGADQFD+  +  K     FF
Sbjct: 134 -----GETCHATAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESKSSFF 193

Query: 236 NFFYLSVTIGAIVAFTAVVYIQMKHGWGAAFGSLAIAMGLSNVVFFTGTPLYRHRLPGGS 295
           N+FY  + +GA++A + +V+IQM  GWG   G   +AM ++ V FF G+  YR + PGGS
Sbjct: 194 NWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQKPGGS 253

Query: 296 PLTRVAQVLVAAFRKRHTSFSSSEMVGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQL 355
           PLTR+ QV+VA+ RK        E + LYE    +S+I GS K+ HT      DKAA++ 
Sbjct: 254 PLTRMLQVIVASCRKSKVKIPEDESL-LYENQDAESSIIGSRKLEHTKILTFFDKAAVET 313

Query: 356 KED---GGNPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNT 415
           + D       S W+LCTVTQVEE+K L++L+PI A  I+   V ++  T+ V Q  T++ 
Sbjct: 314 ESDNKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNTLDQ 373

Query: 416 HMG-RLKLPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISI 475
           HMG   K+P   + +F  LS+     +Y  + VP +R+ TGH  G +QLQR+GIGL ISI
Sbjct: 374 HMGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLVISI 433

Query: 476 ISVAWAGAFERYRRNFAIKNGYEASFLTPMPNLSAFWLLIQYCLIGIAEVFCIVGLLEFL 535
            S+  AG  E  R N+   +        PM   + FW + QY L+G AEVF  +G LEF 
Sbjct: 434 FSMVSAGILEVARLNYVQTHNLYNEETIPM---TIFWQVPQYFLVGCAEVFTFIGQLEFF 493

Query: 536 YEEAPDAMKSIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQNINTGRFDY 595
           Y++APDAM+S+ SA +  A   G + ++ L +++  VT +     P W+++N+N G  DY
Sbjct: 494 YDQAPDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGR--PGWIAKNLNNGHLDY 553

Query: 596 FYWLLTVMSIINFCIFLYSAHRYKYRK 619
           F+WLL  +S +NF ++L+ A  Y Y+K
Sbjct: 554 FFWLLAGLSFLNFLVYLWIAKWYTYKK 564

BLAST of Spg030668 vs. ExPASy Swiss-Prot
Match: Q93Z20 (Protein NRT1/ PTR FAMILY 8.5 OS=Arabidopsis thaliana OX=3702 GN=NPF8.5 PE=2 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 1.0e-103
Identity = 221/576 (38.37%), Postives = 328/576 (56.94%), Query Frame = 0

Query: 46  GRGYTGGTTPVNIRGKPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMH 105
           G   T     ++I G P     KTG W A  FI GNE  ER+AY+G++ N++ +    +H
Sbjct: 31  GLSSTAEDGSIDIYGNP-PSKKKTGNWKACPFILGNECCERLAYYGIAKNLITYYTSELH 90

Query: 106 MPFTTSSNAVNNFLGISQASSVLGGFLADAYLGRYWTIAIFTTVYLGGLTGITLCATISA 165
               ++++ V  + G    + ++G  +AD+Y GRYWTIA F+ +Y  G+  +TL A++  
Sbjct: 91  ESNVSAASDVMIWQGTCYITPLIGAVIADSYWGRYWTIASFSAIYFIGMALLTLSASLPV 150

Query: 166 FVPNQQKCDQLTLLLGKCEPAKSWQMTYLYAVLYLTGFGAAGIRPCVSSFGADQFDEKSK 225
             P    C  +   L  C PA + Q    +  LYL   G  GI+PCVSSFGADQFD+   
Sbjct: 151 LKP--AACAGVAAAL--CSPATTVQYAVFFTGLYLIALGTGGIKPCVSSFGADQFDDTDP 210

Query: 226 DYKSHLDRFFNFFYLSVTIGAIVAFTAVVYIQMKHGWGAAFGSLAIAMGLSNVVFFTGTP 285
             +     FFN+FY S+ IG+ ++ T +V++Q   GWG  F    + MG+S   FF GTP
Sbjct: 211 RERVRKASFFNWFYFSINIGSFISSTLLVWVQENVGWGLGFLIPTVFMGVSIASFFIGTP 270

Query: 286 LYRHRLPGGSPLTRVAQVLVAAFRKRHTSFSSSEMVGLYEVPGKQSAIKGSGKILHTDDF 345
           LYR + PGGSP+TRV QVLVAA+RK   +    ++  LYE   K S I GS KI HTD +
Sbjct: 271 LYRFQKPGGSPITRVCQVLVAAYRKLKLNL-PEDISFLYETREKNSMIAGSRKIQHTDGY 330

Query: 346 RCLDKAAL---QLKEDGGNPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTL 405
           + LDKAA+      + G   +PW+LCTVTQVEEVK L+++ PI A  I+ +++ ++  TL
Sbjct: 331 KFLDKAAVISEYESKSGAFSNPWKLCTVTQVEEVKTLIRMFPIWASGIVYSVLYSQISTL 390

Query: 406 SVQQAYTMNTHMGRLKLPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQR 465
            VQQ  +MN  +   ++P     VF  L + + + +Y    VP  RR TG P G + LQR
Sbjct: 391 FVQQGRSMNRIIRSFEIPPASFGVFDTLIVLISIPIYDRFLVPFVRRFTGIPKGLTDLQR 450

Query: 466 VGIGLAISIISVAWAGAFERYRRNFAIKNGYEASFLTPMPNLSAFWLLIQYCLIGIAEVF 525
           +GIGL +S++S+A A   E  R   A        F+     +S FW + QY L+GIAEVF
Sbjct: 451 MGIGLFLSVLSIAAAAIVETVRLQLA------QDFVA----MSIFWQIPQYILMGIAEVF 510

Query: 526 CIVGLLEFLYEEAPDAMKSIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQ 585
             +G +EF Y+E+PDAM+S+ SA A L   +G + +SL+ +++   T    +    W+  
Sbjct: 511 FFIGRVEFFYDESPDAMRSVCSALALLNTAVGSYLSSLILTLVAYFTALGGK--DGWVPD 570

Query: 586 NINTGRFDYFYWLLTVMSIINFCIFLYSAHRYKYRK 619
           ++N G  DYF+WLL  + ++N  ++     ++  +K
Sbjct: 571 DLNKGHLDYFFWLLVSLGLVNIPVYALICVKHTKKK 588

BLAST of Spg030668 vs. ExPASy TrEMBL
Match: A0A0A0KYN3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G007610 PE=3 SV=1)

HSP 1 Score: 1199.5 bits (3102), Expect = 0.0e+00
Identity = 597/637 (93.72%), Postives = 619/637 (97.17%), Query Frame = 0

Query: 1   MGSREIKSPQEVIEMP--GRVDEHSESFQRKKLGMFFVESDDRRTAFGRGYTGGTTPVNI 60
           MGSREIKS  EV E P  GR+DEHSESFQRKKLGMFF+ESDDRRTAFGRGYTGGTTPVNI
Sbjct: 1   MGSREIKSLHEVNETPVLGRLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNI 60

Query: 61  RGKPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSNAVNNF 120
           RGKPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSS+AVNNF
Sbjct: 61  RGKPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNF 120

Query: 121 LGISQASSVLGGFLADAYLGRYWTIAIFTTVYLGGLTGITLCATISAFVPNQQKCDQLTL 180
           LGISQASSVLGGFLADAYLGRYWTIAIFTT+YLGGL GITLCATIS FVPNQ KCDQL+L
Sbjct: 121 LGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLVGITLCATISTFVPNQAKCDQLSL 180

Query: 181 LLGKCEPAKSWQMTYLYAVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF 240
           LLG+CEPAK WQMTYLY VLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF
Sbjct: 181 LLGRCEPAKPWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF 240

Query: 241 YLSVTIGAIVAFTAVVYIQMKHGWGAAFGSLAIAMGLSNVVFFTGTPLYRHRLPGGSPLT 300
           YLSVT+GAI+AFTAVVYIQ+KHGWGAAFGSLAIAMG SNVVFF GTPLYRHRLPGGSPLT
Sbjct: 241 YLSVTVGAIIAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLT 300

Query: 301 RVAQVLVAAFRKRHTSFSSSEMVGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQLKED 360
           RVAQVLVAA+RKR+ SFS+SE VGL+EVPGKQSAIKGSGKILHTDDFRCLDKAALQLKED
Sbjct: 301 RVAQVLVAAYRKRNASFSNSEFVGLFEVPGKQSAIKGSGKILHTDDFRCLDKAALQLKED 360

Query: 361 GGNPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTHMGRLK 420
           GGNPSPW+LCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTH+GRLK
Sbjct: 361 GGNPSPWKLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTHIGRLK 420

Query: 421 LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAG 480
           LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISI+SVAWAG
Sbjct: 421 LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISILSVAWAG 480

Query: 481 AFERYRRNFAIKNGYEASFLTPMPNLSAFWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA 540
           AFERYRRNFAI++GYEASFL+PMPNLSA+WLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA
Sbjct: 481 AFERYRRNFAIRSGYEASFLSPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA 540

Query: 541 MKSIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQNINTGRFDYFYWLLTV 600
           MKSIGSAYAALAGGLGCFAASLLNSIIKSVTG+PN R PSWLSQNINTGRFDYFYWLLTV
Sbjct: 541 MKSIGSAYAALAGGLGCFAASLLNSIIKSVTGSPNGRNPSWLSQNINTGRFDYFYWLLTV 600

Query: 601 MSIINFCIFLYSAHRYKYRKDHEVGEGIMENGRHDKM 636
           MSIINFCIFLYSAH+YKYRKDHEVGEGIMENG HDKM
Sbjct: 601 MSIINFCIFLYSAHKYKYRKDHEVGEGIMENGMHDKM 637

BLAST of Spg030668 vs. ExPASy TrEMBL
Match: A0A5A7TN06 (Protein NRT1/ PTR FAMILY 6.1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G002320 PE=3 SV=1)

HSP 1 Score: 1198.0 bits (3098), Expect = 0.0e+00
Identity = 596/637 (93.56%), Postives = 619/637 (97.17%), Query Frame = 0

Query: 1   MGSREIKSPQEVIEMP--GRVDEHSESFQRKKLGMFFVESDDRRTAFGRGYTGGTTPVNI 60
           MGSREIKS QEV E P  GR+DEHSESFQRKKLGMFF+ESDDRRTAFGRGYTGGTTPVNI
Sbjct: 1   MGSREIKSLQEVNETPVLGRLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNI 60

Query: 61  RGKPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSNAVNNF 120
           RGKPI DLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFT+SS+AVNNF
Sbjct: 61  RGKPIVDLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTSSSDAVNNF 120

Query: 121 LGISQASSVLGGFLADAYLGRYWTIAIFTTVYLGGLTGITLCATISAFVPNQQKCDQLTL 180
           LGISQASSVLGGFLADAYLGRYWTIAIFTT+YLGGL GITLCATIS FVPNQ KCDQL+L
Sbjct: 121 LGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLVGITLCATISTFVPNQAKCDQLSL 180

Query: 181 LLGKCEPAKSWQMTYLYAVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF 240
           LLG+CEPAKSWQMTYLY VLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF
Sbjct: 181 LLGRCEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF 240

Query: 241 YLSVTIGAIVAFTAVVYIQMKHGWGAAFGSLAIAMGLSNVVFFTGTPLYRHRLPGGSPLT 300
           YLSVT+GAI+AFTAVVYIQ++HGWGAAFGSLAIAMG SNVVFF GTPLYRHRLPGGSPLT
Sbjct: 241 YLSVTVGAIIAFTAVVYIQIQHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLT 300

Query: 301 RVAQVLVAAFRKRHTSFSSSEMVGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQLKED 360
           RVAQVLVAA+RKR+ SFS+SE VGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQLKED
Sbjct: 301 RVAQVLVAAYRKRNASFSNSEFVGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQLKED 360

Query: 361 GGNPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTHMGRLK 420
           GGNPSPW+LCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTH+GRLK
Sbjct: 361 GGNPSPWKLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTHIGRLK 420

Query: 421 LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAG 480
           LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISI+SVAWAG
Sbjct: 421 LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISILSVAWAG 480

Query: 481 AFERYRRNFAIKNGYEASFLTPMPNLSAFWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA 540
           AFERYRRNFA+++GYEASFL+PMPNLSA+WLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA
Sbjct: 481 AFERYRRNFAVRSGYEASFLSPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA 540

Query: 541 MKSIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQNINTGRFDYFYWLLTV 600
           MKSIGSAYAALAGGLGCFAASLLNSIIKSVTG+ N R PSWLSQNINTGRFDYFYWLLTV
Sbjct: 541 MKSIGSAYAALAGGLGCFAASLLNSIIKSVTGSTNGRNPSWLSQNINTGRFDYFYWLLTV 600

Query: 601 MSIINFCIFLYSAHRYKYRKDHEVGEGIMENGRHDKM 636
           MSIINFCIFLYSAHRYKYRKDHEVGEGIMENG HDKM
Sbjct: 601 MSIINFCIFLYSAHRYKYRKDHEVGEGIMENGMHDKM 637

BLAST of Spg030668 vs. ExPASy TrEMBL
Match: A0A1S3BXF6 (protein NRT1/ PTR FAMILY 6.1 OS=Cucumis melo OX=3656 GN=LOC103494642 PE=3 SV=1)

HSP 1 Score: 1197.6 bits (3097), Expect = 0.0e+00
Identity = 596/637 (93.56%), Postives = 619/637 (97.17%), Query Frame = 0

Query: 1   MGSREIKSPQEVIEMP--GRVDEHSESFQRKKLGMFFVESDDRRTAFGRGYTGGTTPVNI 60
           MGSREIKS QEV E P  GR+DEHSESFQRKKLGMFF+ESDDRRTAFGRGYTGGTTPVNI
Sbjct: 1   MGSREIKSLQEVNETPVLGRLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNI 60

Query: 61  RGKPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSNAVNNF 120
           RGKPI DLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFT+SS+AVNNF
Sbjct: 61  RGKPIVDLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTSSSDAVNNF 120

Query: 121 LGISQASSVLGGFLADAYLGRYWTIAIFTTVYLGGLTGITLCATISAFVPNQQKCDQLTL 180
           LGISQASSVLGGFLADAYLGRYWTIAIFTT+YLGGL GITLCATIS FVPNQ KCDQL+L
Sbjct: 121 LGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLVGITLCATISRFVPNQAKCDQLSL 180

Query: 181 LLGKCEPAKSWQMTYLYAVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF 240
           LLG+CEPAKSWQMTYLY VLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF
Sbjct: 181 LLGRCEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF 240

Query: 241 YLSVTIGAIVAFTAVVYIQMKHGWGAAFGSLAIAMGLSNVVFFTGTPLYRHRLPGGSPLT 300
           YLSVT+GAI+AFTAVVYIQ++HGWGAAFGSLAIAMG SNVVFF GTPLYRHRLPGGSPLT
Sbjct: 241 YLSVTVGAIIAFTAVVYIQIQHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLT 300

Query: 301 RVAQVLVAAFRKRHTSFSSSEMVGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQLKED 360
           RVAQVLVAA+RKR+ SFS+SE VGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQLKED
Sbjct: 301 RVAQVLVAAYRKRNASFSNSEFVGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQLKED 360

Query: 361 GGNPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTHMGRLK 420
           GGNPSPW+LCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTH+GRLK
Sbjct: 361 GGNPSPWKLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTHIGRLK 420

Query: 421 LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAG 480
           LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISI+SVAWAG
Sbjct: 421 LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISILSVAWAG 480

Query: 481 AFERYRRNFAIKNGYEASFLTPMPNLSAFWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA 540
           AFERYRRNFA+++GYEASFL+PMPNLSA+WLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA
Sbjct: 481 AFERYRRNFAVRSGYEASFLSPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA 540

Query: 541 MKSIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQNINTGRFDYFYWLLTV 600
           MKSIGSAYAALAGGLGCFAASLLNSIIKSVTG+ N R PSWLSQNINTGRFDYFYWLLTV
Sbjct: 541 MKSIGSAYAALAGGLGCFAASLLNSIIKSVTGSTNGRNPSWLSQNINTGRFDYFYWLLTV 600

Query: 601 MSIINFCIFLYSAHRYKYRKDHEVGEGIMENGRHDKM 636
           MSIINFCIFLYSAHRYKYRKDHEVGEGIMENG HDKM
Sbjct: 601 MSIINFCIFLYSAHRYKYRKDHEVGEGIMENGMHDKM 637

BLAST of Spg030668 vs. ExPASy TrEMBL
Match: A0A5D3E115 (Protein NRT1/ PTR FAMILY 6.1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G002340 PE=3 SV=1)

HSP 1 Score: 1197.6 bits (3097), Expect = 0.0e+00
Identity = 596/637 (93.56%), Postives = 619/637 (97.17%), Query Frame = 0

Query: 1   MGSREIKSPQEVIEMP--GRVDEHSESFQRKKLGMFFVESDDRRTAFGRGYTGGTTPVNI 60
           MGSREIKS QEV E P  GR+DEHSESFQRKKLGMFF+ESDDRRTAFGRGYTGGTTPVNI
Sbjct: 1   MGSREIKSLQEVNETPVLGRLDEHSESFQRKKLGMFFIESDDRRTAFGRGYTGGTTPVNI 60

Query: 61  RGKPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSNAVNNF 120
           RGKPI DLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFT+SS+AVNNF
Sbjct: 61  RGKPIVDLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTSSSDAVNNF 120

Query: 121 LGISQASSVLGGFLADAYLGRYWTIAIFTTVYLGGLTGITLCATISAFVPNQQKCDQLTL 180
           LGISQASSVLGGFLADAYLGRYWTIAIFTT+YLGGL GITLCATIS FVPNQ KCDQL+L
Sbjct: 121 LGISQASSVLGGFLADAYLGRYWTIAIFTTIYLGGLVGITLCATISRFVPNQAKCDQLSL 180

Query: 181 LLGKCEPAKSWQMTYLYAVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF 240
           LLG+CEPAKSWQMTYLY VLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF
Sbjct: 181 LLGRCEPAKSWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFF 240

Query: 241 YLSVTIGAIVAFTAVVYIQMKHGWGAAFGSLAIAMGLSNVVFFTGTPLYRHRLPGGSPLT 300
           YLSVT+GAI+AFTAVVYIQ++HGWGAAFGSLAIAMG SNVVFF GTPLYRHRLPGGSPLT
Sbjct: 241 YLSVTVGAIIAFTAVVYIQIQHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLT 300

Query: 301 RVAQVLVAAFRKRHTSFSSSEMVGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQLKED 360
           RVAQVLVAA+RKR+ SFS+SE VGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQLKED
Sbjct: 301 RVAQVLVAAYRKRNASFSNSEFVGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQLKED 360

Query: 361 GGNPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTHMGRLK 420
           GGNPSPW+LCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTH+GRLK
Sbjct: 361 GGNPSPWKLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTHIGRLK 420

Query: 421 LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAG 480
           LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISI+SVAWAG
Sbjct: 421 LPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISILSVAWAG 480

Query: 481 AFERYRRNFAIKNGYEASFLTPMPNLSAFWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA 540
           AFERYRRNFA+++GYEASFL+PMPNLSA+WLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA
Sbjct: 481 AFERYRRNFAVRSGYEASFLSPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDA 540

Query: 541 MKSIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQNINTGRFDYFYWLLTV 600
           MKSIGSAYAALAGGLGCFAASLLNSIIKSVTG+ N R PSWLSQNINTGRFDYFYWLLTV
Sbjct: 541 MKSIGSAYAALAGGLGCFAASLLNSIIKSVTGSTNGRNPSWLSQNINTGRFDYFYWLLTV 600

Query: 601 MSIINFCIFLYSAHRYKYRKDHEVGEGIMENGRHDKM 636
           MSIINFCIFLYSAHRYKYRKDHEVGEGIMENG HDKM
Sbjct: 601 MSIINFCIFLYSAHRYKYRKDHEVGEGIMENGMHDKM 637

BLAST of Spg030668 vs. ExPASy TrEMBL
Match: A0A6J1I4E7 (protein NRT1/ PTR FAMILY 6.1 OS=Cucurbita maxima OX=3661 GN=LOC111470868 PE=3 SV=1)

HSP 1 Score: 1182.5 bits (3058), Expect = 0.0e+00
Identity = 590/635 (92.91%), Postives = 612/635 (96.38%), Query Frame = 0

Query: 1   MGSREIKSPQEVIEMPGRVDEHSESFQRKKLGMFFVESDDRRTAFGRGYTGGTTPVNIRG 60
           MGS EIKSPQE++EMP R+DE     QRKKLGMFFVESDDRRTAFGRGYTGGTTPVNIRG
Sbjct: 1   MGSTEIKSPQEMVEMPARLDE-----QRKKLGMFFVESDDRRTAFGRGYTGGTTPVNIRG 60

Query: 61  KPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSNAVNNFLG 120
           KPIADLSKTGGW AAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSS+AVNNFLG
Sbjct: 61  KPIADLSKTGGWTAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSDAVNNFLG 120

Query: 121 ISQASSVLGGFLADAYLGRYWTIAIFTTVYLGGLTGITLCATISAFVPNQQKCDQLTLLL 180
           ISQASSVLGGF+ADAYLGRYWTIAIFTT+YLGGLTGITLCATISAFVPNQ+ CD+L+LLL
Sbjct: 121 ISQASSVLGGFIADAYLGRYWTIAIFTTIYLGGLTGITLCATISAFVPNQKICDELSLLL 180

Query: 181 GKCEPAKSWQMTYLYAVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYL 240
           GKCEPAK+WQMTYLY VLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYL
Sbjct: 181 GKCEPAKAWQMTYLYTVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYL 240

Query: 241 SVTIGAIVAFTAVVYIQMKHGWGAAFGSLAIAMGLSNVVFFTGTPLYRHRLPGGSPLTRV 300
           SVT GAIVAFTAVVYIQ+KHGWGAAFGSLAIAMG SNVVFF GTPLYRHRLPGGSPLTRV
Sbjct: 241 SVTTGAIVAFTAVVYIQIKHGWGAAFGSLAIAMGFSNVVFFLGTPLYRHRLPGGSPLTRV 300

Query: 301 AQVLVAAFRKRHTSFSSSEMVGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQLKEDGG 360
           AQVLVAAFRKR+ SFSSSEMVGLYEV GK SAIKGSGKILHT+DFRCLDKAAL+LK+DGG
Sbjct: 301 AQVLVAAFRKRNASFSSSEMVGLYEVAGKHSAIKGSGKILHTNDFRCLDKAALELKDDGG 360

Query: 361 NPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTHMGRLKLP 420
           NPSPWRLCTVTQVEEVKILLKLIPIPACTI+LNLVLTEYLTLSVQQAYT+NTHMGRLKLP
Sbjct: 361 NPSPWRLCTVTQVEEVKILLKLIPIPACTIILNLVLTEYLTLSVQQAYTLNTHMGRLKLP 420

Query: 421 VTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAGAF 480
           VTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAG F
Sbjct: 421 VTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAGVF 480

Query: 481 ERYRRNFAIKNGYEASFLTPMPNLSAFWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMK 540
           ERYRRN+AIK+GYEASFLTPMPNLSA+WLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMK
Sbjct: 481 ERYRRNYAIKSGYEASFLTPMPNLSAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMK 540

Query: 541 SIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQNINTGRFDYFYWLLTVMS 600
           SIGS+YAALAGGLGCFAASLLNSIIKSVTGNP E  PSWLSQNIN G+FDYFYWLLTVMS
Sbjct: 541 SIGSSYAALAGGLGCFAASLLNSIIKSVTGNPKEGTPSWLSQNINKGQFDYFYWLLTVMS 600

Query: 601 IINFCIFLYSAHRYKYRKDHEVGEGIMENGRHDKM 636
           IINFCIFLYSAHRYKYRKDHEVGEGIMENGRH  M
Sbjct: 601 IINFCIFLYSAHRYKYRKDHEVGEGIMENGRHGTM 630

BLAST of Spg030668 vs. TAIR 10
Match: AT5G13400.1 (Major facilitator superfamily protein )

HSP 1 Score: 988.4 bits (2554), Expect = 2.6e-288
Identity = 492/617 (79.74%), Postives = 553/617 (89.63%), Query Frame = 0

Query: 1   MGSREIKSPQEVIEMPGRVDEHSESFQRKKLGMFFVESDDRRTAFGRGYTGGTTPVNIRG 60
           M + EIKSP  V E PG    H     RK+L +FF+ESD+RR A GRGYTGGTTPVNI G
Sbjct: 1   MVASEIKSPVSVPETPGSSSVH----HRKQLSVFFIESDNRRLALGRGYTGGTTPVNIHG 60

Query: 61  KPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSNAVNNFLG 120
           KPIA+LSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMH PF +SSNAVNNFLG
Sbjct: 61  KPIANLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHRPFESSSNAVNNFLG 120

Query: 121 ISQASSVLGGFLADAYLGRYWTIAIFTTVYLGGLTGITLCATISAFVPNQQKCDQLTLLL 180
           ISQASSVLGGFLADAYLGRYWTIAIFTT+YL GL GITL A++  FVP+Q  C QL+LLL
Sbjct: 121 ISQASSVLGGFLADAYLGRYWTIAIFTTMYLVGLIGITLGASLKMFVPDQSNCGQLSLLL 180

Query: 181 GKCEPAKSWQMTYLYAVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFFNFFYL 240
           G CE AKSWQM YLY VLY+TGFGAAGIRPCVSSFGADQFDEKSKDYK+HLDRFFNFFYL
Sbjct: 181 GNCEEAKSWQMLYLYTVLYITGFGAAGIRPCVSSFGADQFDEKSKDYKTHLDRFFNFFYL 240

Query: 241 SVTIGAIVAFTAVVYIQMKHGWGAAFGSLAIAMGLSNVVFFTGTPLYRHRLPGGSPLTRV 300
           SVT+GAI+AFT VVY+QM+ GWG AFG+LA+AMG+SN +FF GTPLYRHRLPGGSPLTRV
Sbjct: 241 SVTLGAIIAFTLVVYVQMELGWGMAFGTLAVAMGISNALFFAGTPLYRHRLPGGSPLTRV 300

Query: 301 AQVLVAAFRKRHTSFSSSEMVGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQLKEDGG 360
           AQVLVAAFRKR+ +F+SSE +GLYEVPG +SAI GS KI H++DF  LDKAAL+LKEDG 
Sbjct: 301 AQVLVAAFRKRNAAFTSSEFIGLYEVPGLKSAINGSRKIPHSNDFIWLDKAALELKEDGL 360

Query: 361 NPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNTHMGRLKLP 420
            PSPW+LCTVTQVEEVKIL++LIPIP CTIML+LVLTEYLTLSVQQAYT+NTH+  LKLP
Sbjct: 361 EPSPWKLCTVTQVEEVKILIRLIPIPTCTIMLSLVLTEYLTLSVQQAYTLNTHIQHLKLP 420

Query: 421 VTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISIISVAWAGAF 480
           VTCMPVFPGLSIFLILSLYYSVFVP++RRITG+PHGASQLQRVGIGLA+SIISVAWAG F
Sbjct: 421 VTCMPVFPGLSIFLILSLYYSVFVPITRRITGNPHGASQLQRVGIGLAVSIISVAWAGLF 480

Query: 481 ERYRRNFAIKNGYEASFLTPMPNLSAFWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMK 540
           E YRR++AI+NG+E +FLT MP+L+A+WLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMK
Sbjct: 481 ENYRRHYAIQNGFEFNFLTQMPDLTAYWLLIQYCLIGIAEVFCIVGLLEFLYEEAPDAMK 540

Query: 541 SIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQNINTGRFDYFYWLLTVMS 600
           SIGSAYAALAGGLGCFAA++LN+I+K+ T + + +  SWLSQNINTGRFD  YWLLT++S
Sbjct: 541 SIGSAYAALAGGLGCFAATILNNIVKAATRDSDGK--SWLSQNINTGRFDCLYWLLTLLS 600

Query: 601 IINFCIFLYSAHRYKYR 618
            +NFC+FL+SAHRYKYR
Sbjct: 601 FLNFCVFLWSAHRYKYR 611

BLAST of Spg030668 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 421.4 bits (1082), Expect = 1.3e-117
Identity = 229/568 (40.32%), Postives = 344/568 (60.56%), Query Frame = 0

Query: 56  VNIRGKPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSNAV 115
           V+I   P A+  KTG W A  FI GNE  ER+AY+G+  N+V ++   ++    T++N V
Sbjct: 13  VDIHKNP-ANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGNATAANNV 72

Query: 116 NNFLGISQASSVLGGFLADAYLGRYWTIAIFTTVYLGGLTGITLCATISAFVPNQQKCDQ 175
            N+ G    + ++G F+ADAYLGRYWTIA F  +Y+ G+T +TL A++    P     D 
Sbjct: 73  TNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKPGNCNAD- 132

Query: 176 LTLLLGKCEPAKSWQMTYLYAVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFF 235
                  C P  S Q    +  LY+   G  GI+PCVSSFGADQFDE  ++ K     FF
Sbjct: 133 ------TCHPNSS-QTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFF 192

Query: 236 NFFYLSVTIGAIVAFTAVVYIQMKHGWGAAFGSLAIAMGLSNVVFFTGTPLYRHRLPGGS 295
           N+FY S+ +GA++A T +V+IQM  GWG  FG   +AM ++   FF G+  YR + PGGS
Sbjct: 193 NWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGS 252

Query: 296 PLTRVAQVLVAAFRKRHTSFSSSEMVGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQL 355
           PLTR+ QV+VAAFRK        + + L+E    +S IKGS K++HTD+ +  DKAA++ 
Sbjct: 253 PLTRIFQVIVAAFRKISVKVPEDKSL-LFETADDESNIKGSRKLVHTDNLKFFDKAAVES 312

Query: 356 KEDG---GNPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNT 415
           + D    G  +PWRLC+VTQVEE+K ++ L+P+ A  I+   V ++  T+ V Q  TM+ 
Sbjct: 313 QSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQ 372

Query: 416 HMGR-LKLPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISI 475
           HMG+  ++P   + +F  +S+     +Y    +PL+R+ T +  G +QLQR+GIGL +SI
Sbjct: 373 HMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVVSI 432

Query: 476 ISVAWAGAFERYRRNFA-IKNGYEASFLTPMPNLSAFWLLIQYCLIGIAEVFCIVGLLEF 535
            ++  AG  E  R ++    N Y+   +    ++S FW + QY LIG AEVF  +G LEF
Sbjct: 433 FAMITAGVLEVVRLDYVKTHNAYDQKQI----HMSIFWQIPQYLLIGCAEVFTFIGQLEF 492

Query: 536 LYEEAPDAMKSIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQNINTGRFD 595
            Y++APDAM+S+ SA +     LG + +++L +++  +T    +  P W+  N+N G  D
Sbjct: 493 FYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGK--PGWIPDNLNRGHLD 552

Query: 596 YFYWLLTVMSIINFCIFLYSAHRYKYRK 619
           YF++LL  +S +NF ++L+ + RYKY+K
Sbjct: 553 YFFYLLATLSFLNFLVYLWISKRYKYKK 564

BLAST of Spg030668 vs. TAIR 10
Match: AT2G02040.1 (peptide transporter 2 )

HSP 1 Score: 413.7 bits (1062), Expect = 2.7e-115
Identity = 232/566 (40.99%), Postives = 336/566 (59.36%), Query Frame = 0

Query: 56  VNIRGKPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSNAV 115
           V+  G P     KTG W A  FI GNE  ER+AY+G++ N++ ++   +H    +++  V
Sbjct: 30  VDFNGNPPLK-EKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLHQGNVSAATNV 89

Query: 116 NNFLGISQASSVLGGFLADAYLGRYWTIAIFTTVYLGGLTGITLCATISAFVPNQQKCDQ 175
             + G    + ++G  LADAY GRYWTIA F+ +Y  G++ +TL A++ A  P +   D 
Sbjct: 90  TTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKPAECIGD- 149

Query: 176 LTLLLGKCEPAKSWQMTYLYAVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFF 235
                  C  A   Q    +  LYL   G  GI+PCVSSFGADQFD+     +     FF
Sbjct: 150 ------FCPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKASFF 209

Query: 236 NFFYLSVTIGAIVAFTAVVYIQMKHGWGAAFGSLAIAMGLSNVVFFTGTPLYRHRLPGGS 295
           N+FY S+ IGA+V+ + +V+IQ   GWG  FG   + MGL+   FF GTPLYR + PGGS
Sbjct: 210 NWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKPGGS 269

Query: 296 PLTRVAQVLVAAFRKRHTSFSSSEMVGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQL 355
           P+TR++QV+VA+FRK          + LYE   K SAI GS KI HTDD + LDKAA+  
Sbjct: 270 PITRISQVVVASFRKSSVKVPEDATL-LYETQDKNSAIAGSRKIEHTDDCQYLDKAAVIS 329

Query: 356 KED---GGNPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNT 415
           +E+   G   + WRLCTVTQVEE+KIL+++ PI A  I+ + V  +  T+ VQQ   MN 
Sbjct: 330 EEESKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGRAMNC 389

Query: 416 HMGRLKLPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISII 475
            +G  +LP   +  F   S+ + + LY    VPL+R+ TG   G +++QR+GIGL +S++
Sbjct: 390 KIGSFQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGLFVSVL 449

Query: 476 SVAWAGAFERYRRNFAIKNGYEASFLTPMPNLSAFWLLIQYCLIGIAEVFCIVGLLEFLY 535
            +A A   E  R + A   G   S   P+P +S  W + QY ++G AEVF  +G LEF Y
Sbjct: 450 CMAAAAIVEIIRLHMANDLGLVESG-APVP-ISVLWQIPQYFILGAAEVFYFIGQLEFFY 509

Query: 536 EEAPDAMKSIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQNINTGRFDYF 595
           +++PDAM+S+ SA A L   LG + +SL+ +++   T    +    W+S N+N+G  DYF
Sbjct: 510 DQSPDAMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNGQE--GWISDNLNSGHLDYF 569

Query: 596 YWLLTVMSIINFCIFLYSAHRYKYRK 619
           +WLL  +S++N  ++ +SA RYK +K
Sbjct: 570 FWLLAGLSLVNMAVYFFSAARYKQKK 582

BLAST of Spg030668 vs. TAIR 10
Match: AT5G01180.1 (peptide transporter 5 )

HSP 1 Score: 410.6 bits (1054), Expect = 2.3e-114
Identity = 230/567 (40.56%), Postives = 338/567 (59.61%), Query Frame = 0

Query: 56  VNIRGKPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMHMPFTTSSNAV 115
           ++I  KP A+ +KTG W A  FI G E  ER+AY+G+S N++ ++   M+M   ++S +V
Sbjct: 14  LDIHKKP-ANKNKTGTWKACRFILGTECCERLAYYGMSTNLINYLEKQMNMENVSASKSV 73

Query: 116 NNFLGISQASSVLGGFLADAYLGRYWTIAIFTTVYLGGLTGITLCATISAFVPNQQKCDQ 175
           +N+ G   A+ ++G F+ADAYLGRYWTIA F  +Y+ G+T +T+ A++    P    C  
Sbjct: 74  SNWSGTCYATPLIGAFIADAYLGRYWTIASFVVIYIAGMTLLTISASVPGLTPT---CS- 133

Query: 176 LTLLLGKCEPAKSWQMTYLYAVLYLTGFGAAGIRPCVSSFGADQFDEKSKDYKSHLDRFF 235
                G+   A + Q    +  LYL   G  GI+PCVSSFGADQFD+  +  K     FF
Sbjct: 134 -----GETCHATAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESKSSFF 193

Query: 236 NFFYLSVTIGAIVAFTAVVYIQMKHGWGAAFGSLAIAMGLSNVVFFTGTPLYRHRLPGGS 295
           N+FY  + +GA++A + +V+IQM  GWG   G   +AM ++ V FF G+  YR + PGGS
Sbjct: 194 NWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQKPGGS 253

Query: 296 PLTRVAQVLVAAFRKRHTSFSSSEMVGLYEVPGKQSAIKGSGKILHTDDFRCLDKAALQL 355
           PLTR+ QV+VA+ RK        E + LYE    +S+I GS K+ HT      DKAA++ 
Sbjct: 254 PLTRMLQVIVASCRKSKVKIPEDESL-LYENQDAESSIIGSRKLEHTKILTFFDKAAVET 313

Query: 356 KED---GGNPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTLSVQQAYTMNT 415
           + D       S W+LCTVTQVEE+K L++L+PI A  I+   V ++  T+ V Q  T++ 
Sbjct: 314 ESDNKGAAKSSSWKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNTLDQ 373

Query: 416 HMG-RLKLPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQRVGIGLAISI 475
           HMG   K+P   + +F  LS+     +Y  + VP +R+ TGH  G +QLQR+GIGL ISI
Sbjct: 374 HMGPNFKIPSASLSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLVISI 433

Query: 476 ISVAWAGAFERYRRNFAIKNGYEASFLTPMPNLSAFWLLIQYCLIGIAEVFCIVGLLEFL 535
            S+  AG  E  R N+   +        PM   + FW + QY L+G AEVF  +G LEF 
Sbjct: 434 FSMVSAGILEVARLNYVQTHNLYNEETIPM---TIFWQVPQYFLVGCAEVFTFIGQLEFF 493

Query: 536 YEEAPDAMKSIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQNINTGRFDY 595
           Y++APDAM+S+ SA +  A   G + ++ L +++  VT +     P W+++N+N G  DY
Sbjct: 494 YDQAPDAMRSLCSALSLTAIAFGNYLSTFLVTLVTKVTRSGGR--PGWIAKNLNNGHLDY 553

Query: 596 FYWLLTVMSIINFCIFLYSAHRYKYRK 619
           F+WLL  +S +NF ++L+ A  Y Y+K
Sbjct: 554 FFWLLAGLSFLNFLVYLWIAKWYTYKK 564

BLAST of Spg030668 vs. TAIR 10
Match: AT1G62200.1 (Major facilitator superfamily protein )

HSP 1 Score: 379.0 bits (972), Expect = 7.4e-105
Identity = 221/576 (38.37%), Postives = 328/576 (56.94%), Query Frame = 0

Query: 46  GRGYTGGTTPVNIRGKPIADLSKTGGWIAAFFIFGNEMAERMAYFGLSVNMVAFMFYVMH 105
           G   T     ++I G P     KTG W A  FI GNE  ER+AY+G++ N++ +    +H
Sbjct: 31  GLSSTAEDGSIDIYGNP-PSKKKTGNWKACPFILGNECCERLAYYGIAKNLITYYTSELH 90

Query: 106 MPFTTSSNAVNNFLGISQASSVLGGFLADAYLGRYWTIAIFTTVYLGGLTGITLCATISA 165
               ++++ V  + G    + ++G  +AD+Y GRYWTIA F+ +Y  G+  +TL A++  
Sbjct: 91  ESNVSAASDVMIWQGTCYITPLIGAVIADSYWGRYWTIASFSAIYFIGMALLTLSASLPV 150

Query: 166 FVPNQQKCDQLTLLLGKCEPAKSWQMTYLYAVLYLTGFGAAGIRPCVSSFGADQFDEKSK 225
             P    C  +   L  C PA + Q    +  LYL   G  GI+PCVSSFGADQFD+   
Sbjct: 151 LKP--AACAGVAAAL--CSPATTVQYAVFFTGLYLIALGTGGIKPCVSSFGADQFDDTDP 210

Query: 226 DYKSHLDRFFNFFYLSVTIGAIVAFTAVVYIQMKHGWGAAFGSLAIAMGLSNVVFFTGTP 285
             +     FFN+FY S+ IG+ ++ T +V++Q   GWG  F    + MG+S   FF GTP
Sbjct: 211 RERVRKASFFNWFYFSINIGSFISSTLLVWVQENVGWGLGFLIPTVFMGVSIASFFIGTP 270

Query: 286 LYRHRLPGGSPLTRVAQVLVAAFRKRHTSFSSSEMVGLYEVPGKQSAIKGSGKILHTDDF 345
           LYR + PGGSP+TRV QVLVAA+RK   +    ++  LYE   K S I GS KI HTD +
Sbjct: 271 LYRFQKPGGSPITRVCQVLVAAYRKLKLNL-PEDISFLYETREKNSMIAGSRKIQHTDGY 330

Query: 346 RCLDKAAL---QLKEDGGNPSPWRLCTVTQVEEVKILLKLIPIPACTIMLNLVLTEYLTL 405
           + LDKAA+      + G   +PW+LCTVTQVEEVK L+++ PI A  I+ +++ ++  TL
Sbjct: 331 KFLDKAAVISEYESKSGAFSNPWKLCTVTQVEEVKTLIRMFPIWASGIVYSVLYSQISTL 390

Query: 406 SVQQAYTMNTHMGRLKLPVTCMPVFPGLSIFLILSLYYSVFVPLSRRITGHPHGASQLQR 465
            VQQ  +MN  +   ++P     VF  L + + + +Y    VP  RR TG P G + LQR
Sbjct: 391 FVQQGRSMNRIIRSFEIPPASFGVFDTLIVLISIPIYDRFLVPFVRRFTGIPKGLTDLQR 450

Query: 466 VGIGLAISIISVAWAGAFERYRRNFAIKNGYEASFLTPMPNLSAFWLLIQYCLIGIAEVF 525
           +GIGL +S++S+A A   E  R   A        F+     +S FW + QY L+GIAEVF
Sbjct: 451 MGIGLFLSVLSIAAAAIVETVRLQLA------QDFVA----MSIFWQIPQYILMGIAEVF 510

Query: 526 CIVGLLEFLYEEAPDAMKSIGSAYAALAGGLGCFAASLLNSIIKSVTGNPNERIPSWLSQ 585
             +G +EF Y+E+PDAM+S+ SA A L   +G + +SL+ +++   T    +    W+  
Sbjct: 511 FFIGRVEFFYDESPDAMRSVCSALALLNTAVGSYLSSLILTLVAYFTALGGK--DGWVPD 570

Query: 586 NINTGRFDYFYWLLTVMSIINFCIFLYSAHRYKYRK 619
           ++N G  DYF+WLL  + ++N  ++     ++  +K
Sbjct: 571 DLNKGHLDYFFWLLVSLGLVNIPVYALICVKHTKKK 588

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038903063.10.0e+0094.66protein NRT1/ PTR FAMILY 6.1 isoform X1 [Benincasa hispida][more]
XP_011653004.10.0e+0093.72protein NRT1/ PTR FAMILY 6.1 [Cucumis sativus] >XP_031740724.1 protein NRT1/ PTR... [more]
KAA0044494.10.0e+0093.56protein NRT1/ PTR FAMILY 6.1 [Cucumis melo var. makuwa][more]
XP_008454154.10.0e+0093.56PREDICTED: protein NRT1/ PTR FAMILY 6.1 [Cucumis melo] >XP_008454155.1 PREDICTED... [more]
XP_022972287.10.0e+0092.91protein NRT1/ PTR FAMILY 6.1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9LYR63.7e-28779.74Protein NRT1/ PTR FAMILY 6.1 OS=Arabidopsis thaliana OX=3702 GN=NPF6.1 PE=2 SV=1[more]
Q9M3901.8e-11640.32Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1[more]
P460323.8e-11440.99Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1[more]
Q9LFB83.2e-11340.56Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1[more]
Q93Z201.0e-10338.37Protein NRT1/ PTR FAMILY 8.5 OS=Arabidopsis thaliana OX=3702 GN=NPF8.5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KYN30.0e+0093.72Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G007610 PE=3 SV=1[more]
A0A5A7TN060.0e+0093.56Protein NRT1/ PTR FAMILY 6.1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca... [more]
A0A1S3BXF60.0e+0093.56protein NRT1/ PTR FAMILY 6.1 OS=Cucumis melo OX=3656 GN=LOC103494642 PE=3 SV=1[more]
A0A5D3E1150.0e+0093.56Protein NRT1/ PTR FAMILY 6.1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A6J1I4E70.0e+0092.91protein NRT1/ PTR FAMILY 6.1 OS=Cucurbita maxima OX=3661 GN=LOC111470868 PE=3 SV... [more]
Match NameE-valueIdentityDescription
AT5G13400.12.6e-28879.74Major facilitator superfamily protein [more]
AT3G54140.11.3e-11740.32peptide transporter 1 [more]
AT2G02040.12.7e-11540.99peptide transporter 2 [more]
AT5G01180.12.3e-11440.56peptide transporter 5 [more]
AT1G62200.17.4e-10538.37Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 140..571
e-value: 8.0E-81
score: 271.9
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 61..617
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 51..622
e-value: 2.1E-164
score: 549.6
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 66..618
NoneNo IPR availablePANTHERPTHR11654:SF112PROTEIN NRT1/ PTR FAMILY 6.1coord: 61..617
NoneNo IPR availableCDDcd17351MFS_NPFcoord: 75..613
e-value: 0.0
score: 554.54
IPR018456PTR2 family proton/oligopeptide symporter, conserved sitePROSITEPS01022PTR2_1coord: 129..153

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg030668.1Spg030668.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006857 oligopeptide transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity