Spg030409 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg030409
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionAdenylyltransferase and sulfurtransferase MOCS3
Locationscaffold6: 26136551 .. 26137332 (-)
RNA-Seq ExpressionSpg030409
SyntenySpg030409
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTGGAATTCCGAATTCCGTTAGCAGAAGCGCAATTGGACCGATTCGCAATTTAATTTCGTTCAACAAAATACCGTACATTGGCAAAAAGTTAAGCAATGAGAAACATACCTCCGTTGACGACTCTGAGAATCCAGAACACTTCTTGATTATGCCGCTACAAGGAACACCACCATTTGGTTTCCCCGCTATGACTCCGAGGTTGGGAATAAAGACAATAATGACAACATATTGACAAATAATATATACAATTGTAAATATTAGAAATTCCAACAAATAATAATGGGTCTAAAATTATCTTTGCGATACAATGCTCAAAGGACAAAATAACCCTTCTGGAGTCAACTCGAGGAGGTCAGAACTAATTTCGAGCTGACTTTTGCATCGCTTCCTTTCTGCTTCGATGTTCTTTGGGTTATACGTTTCCTATGTTTTAGCGTAGCATTCGTCCCACTCTATTTGTGAACTTAGCTTATCAGCATGCGCTCGAGTACTACAGATTCTTCTCGTATTCTTCAAGAAATCGAGACCTTGAAATCTACCAAGTCTGACTTAGAACGTCGTATTTCGGCTCTTGAATCCCAGCTCCGAGACCTGAACCGTCACCAGAAGGATGGCCTGTCCAATGGCTCTTGCACTGCAGTATCCACTCTTGAGTCCGCCTTCCCTCACGAATTGTCCCCCGATATGATGTACAGATACAGTCGCCACCTCCTTCTTCCTTCCTTCGGAATCCAAGATCTACTTTCATTATCAATTCGTTCTTTTCTGAGCCAATAA

mRNA sequence

ATGATTGGAATTCCGAATTCCGTTAGCAGAAGCGCAATTGGACCGATTCGCAATTTAATTTCGTTCAACAAAATACCGTACATTGGCAAAAAGTTAAGCAATGAGAAACATACCTCCGTTGACGACTCTGAGAATCCAGAACACTTCTTGATTATGCCGCTACAAGGAACACCACCATTTGGTTTCCCCGCTATGACTCCGAGCATGCGCTCGAGTACTACAGATTCTTCTCGTATTCTTCAAGAAATCGAGACCTTGAAATCTACCAAGTCTGACTTAGAACGTCGTATTTCGGCTCTTGAATCCCAGCTCCGAGACCTGAACCGTCACCAGAAGGATGGCCTGTCCAATGGCTCTTGCACTGCAGTATCCACTCTTGAGTCCGCCTTCCCTCACGAATTGTCCCCCGATATGATGTACAGATACAGTCGCCACCTCCTTCTTCCTTCCTTCGGAATCCAAGATCTACTTTCATTATCAATTCGTTCTTTTCTGAGCCAATAA

Coding sequence (CDS)

ATGATTGGAATTCCGAATTCCGTTAGCAGAAGCGCAATTGGACCGATTCGCAATTTAATTTCGTTCAACAAAATACCGTACATTGGCAAAAAGTTAAGCAATGAGAAACATACCTCCGTTGACGACTCTGAGAATCCAGAACACTTCTTGATTATGCCGCTACAAGGAACACCACCATTTGGTTTCCCCGCTATGACTCCGAGCATGCGCTCGAGTACTACAGATTCTTCTCGTATTCTTCAAGAAATCGAGACCTTGAAATCTACCAAGTCTGACTTAGAACGTCGTATTTCGGCTCTTGAATCCCAGCTCCGAGACCTGAACCGTCACCAGAAGGATGGCCTGTCCAATGGCTCTTGCACTGCAGTATCCACTCTTGAGTCCGCCTTCCCTCACGAATTGTCCCCCGATATGATGTACAGATACAGTCGCCACCTCCTTCTTCCTTCCTTCGGAATCCAAGATCTACTTTCATTATCAATTCGTTCTTTTCTGAGCCAATAA

Protein sequence

MIGIPNSVSRSAIGPIRNLISFNKIPYIGKKLSNEKHTSVDDSENPEHFLIMPLQGTPPFGFPAMTPSMRSSTTDSSRILQEIETLKSTKSDLERRISALESQLRDLNRHQKDGLSNGSCTAVSTLESAFPHELSPDMMYRYSRHLLLPSFGIQDLLSLSIRSFLSQ
Homology
BLAST of Spg030409 vs. NCBI nr
Match: XP_022158874.1 (adenylyltransferase and sulfurtransferase MOCS3 [Momordica charantia])

HSP 1 Score: 139.8 bits (351), Expect = 2.1e-29
Identity = 69/86 (80.23%), Postives = 79/86 (91.86%), Query Frame = 0

Query: 69  MRSSTTDSSRILQEIETLKSTKSDLERRISALESQLRDLNRHQKDGLSNGSCTAVSTLES 128
           M  ++T+SSRILQ+IETLKS KSDLERRISALESQLRDLN HQ DG+SNGSCT++ST+ES
Sbjct: 1   MGLTSTESSRILQDIETLKSAKSDLERRISALESQLRDLNHHQNDGVSNGSCTSLSTVES 60

Query: 129 AFPHELSPDMMYRYSRHLLLPSFGIQ 155
            FPH LSPDM+YRYSRHLLLPSFG+Q
Sbjct: 61  GFPHGLSPDMIYRYSRHLLLPSFGVQ 86

BLAST of Spg030409 vs. NCBI nr
Match: XP_023007816.1 (adenylyltransferase and sulfurtransferase MOCS3 [Cucurbita maxima])

HSP 1 Score: 127.1 bits (318), Expect = 1.4e-25
Identity = 63/86 (73.26%), Postives = 75/86 (87.21%), Query Frame = 0

Query: 69  MRSSTTDSSRILQEIETLKSTKSDLERRISALESQLRDLNRHQKDGLSNGSCTAVSTLES 128
           M S+TT+SSRIL EIETLKS +SDL+RRIS+LESQLRDLN HQ +G+S+    +VSTL+S
Sbjct: 1   MASTTTESSRILHEIETLKSARSDLDRRISSLESQLRDLNHHQNNGVSHAPSASVSTLDS 60

Query: 129 AFPHELSPDMMYRYSRHLLLPSFGIQ 155
            FPH LSPDM+YRYSRHLLLPSFG+Q
Sbjct: 61  TFPHGLSPDMIYRYSRHLLLPSFGVQ 86

BLAST of Spg030409 vs. NCBI nr
Match: XP_022932280.1 (adenylyltransferase and sulfurtransferase MOCS3 [Cucurbita moschata])

HSP 1 Score: 125.9 bits (315), Expect = 3.2e-25
Identity = 62/86 (72.09%), Postives = 75/86 (87.21%), Query Frame = 0

Query: 69  MRSSTTDSSRILQEIETLKSTKSDLERRISALESQLRDLNRHQKDGLSNGSCTAVSTLES 128
           M S+TT+SSRIL EIETLKS +SDL+RRIS+LESQLRDLN HQ +G+S+    +VSTL+S
Sbjct: 1   MASTTTESSRILHEIETLKSARSDLDRRISSLESQLRDLNHHQNNGVSHAPSASVSTLDS 60

Query: 129 AFPHELSPDMMYRYSRHLLLPSFGIQ 155
            FPH LSPDM+YRYSRHLLLP+FG+Q
Sbjct: 61  TFPHGLSPDMIYRYSRHLLLPTFGVQ 86

BLAST of Spg030409 vs. NCBI nr
Match: KAG7015532.1 (Adenylyltransferase and sulfurtransferase MOCS3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 125.9 bits (315), Expect = 3.2e-25
Identity = 62/86 (72.09%), Postives = 75/86 (87.21%), Query Frame = 0

Query: 69  MRSSTTDSSRILQEIETLKSTKSDLERRISALESQLRDLNRHQKDGLSNGSCTAVSTLES 128
           M S+TT+SSRIL EIETLKS +SDL+RRIS+LESQLRDLN HQ +G+S+    +VSTL+S
Sbjct: 1   MASTTTESSRILHEIETLKSARSDLDRRISSLESQLRDLNHHQNNGVSHAPSASVSTLDS 60

Query: 129 AFPHELSPDMMYRYSRHLLLPSFGIQ 155
            FPH LSPDM+YRYSRHLLLP+FG+Q
Sbjct: 61  TFPHGLSPDMIYRYSRHLLLPTFGVQ 86

BLAST of Spg030409 vs. NCBI nr
Match: XP_023553239.1 (adenylyltransferase and sulfurtransferase MOCS3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 124.8 bits (312), Expect = 7.1e-25
Identity = 61/86 (70.93%), Postives = 75/86 (87.21%), Query Frame = 0

Query: 69  MRSSTTDSSRILQEIETLKSTKSDLERRISALESQLRDLNRHQKDGLSNGSCTAVSTLES 128
           M S+TT+SSRIL EIETLKS +SDL+RRIS+LESQLRDLN HQ +G+S+    ++STL+S
Sbjct: 1   MASTTTESSRILHEIETLKSARSDLDRRISSLESQLRDLNHHQNNGVSHAPSASLSTLDS 60

Query: 129 AFPHELSPDMMYRYSRHLLLPSFGIQ 155
            FPH LSPDM+YRYSRHLLLP+FG+Q
Sbjct: 61  TFPHGLSPDMIYRYSRHLLLPTFGVQ 86

BLAST of Spg030409 vs. ExPASy Swiss-Prot
Match: Q9ZNW0 (Adenylyltransferase and sulfurtransferase MOCS3 OS=Arabidopsis thaliana OX=3702 GN=MOCS3 PE=2 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.1e-09
Identity = 45/99 (45.45%), Postives = 61/99 (61.62%), Query Frame = 0

Query: 69  MRSSTTDSSRILQEIETLKSTKSDLERRISALESQLRDLNRHQ-KDGLSNG-SCTAVSTL 128
           M S+  DSS I++E+E LK  K+++E RIS LE++L+D    +  D +SNG S      L
Sbjct: 1   MMSNGGDSSEIVRELEELKLKKAEIEHRISTLEAKLQDTAAVELYDAVSNGDSYLTAPEL 60

Query: 129 ESAFPHELSPDMMYRYSRHLLLPSFGIQDLLSLSIRSFL 166
           E    H LSPD +YRYSR LLLPSF ++   +L   S L
Sbjct: 61  E----HGLSPDQIYRYSRQLLLPSFAVEGQSNLLKSSVL 95

BLAST of Spg030409 vs. ExPASy Swiss-Prot
Match: B6TNK6 (Adenylyltransferase and sulfurtransferase MOCS3-1 OS=Zea mays OX=4577 GN=MOCS3-1 PE=2 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 3.8e-05
Identity = 34/98 (34.69%), Postives = 51/98 (52.04%), Query Frame = 0

Query: 79  ILQEIETLKSTKSDLERRISALESQLRD-----LNRHQKDGLSNGSCTAVSTLESAFPHE 138
           I +E++ L++ + +L+ RI  LESQL              G+ + +C      +S   +E
Sbjct: 8   IERELKKLRAEREELDGRIRLLESQLEAGPAGFNGAAAGKGVGDDACGGSGACQSRVGNE 67

Query: 139 LSP------DMMYRYSRHLLLPSFGIQDLLSLSIRSFL 166
            +P      DM+YRYSRHLLLP FG++    LS  S L
Sbjct: 68  FAPDGSLPADMIYRYSRHLLLPDFGVEGQRKLSQSSIL 105

BLAST of Spg030409 vs. ExPASy Swiss-Prot
Match: B4FAT0 (Adenylyltransferase and sulfurtransferase MOCS3-2 OS=Zea mays OX=4577 GN=MOCS3-2 PE=2 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 1.4e-04
Identity = 35/98 (35.71%), Postives = 50/98 (51.02%), Query Frame = 0

Query: 79  ILQEIETLKSTKSDLERRISALESQLR---------DLNRHQKDGLSNGSCTAVSTLESA 138
           I +E++ L++ + +L+ RI  LESQL             +   D    GS    S + + 
Sbjct: 22  IERELKKLRAEREELDGRIRLLESQLEAGPAGFNGAAAGKGVGDDACGGSGACQSRVGNG 81

Query: 139 FPHE--LSPDMMYRYSRHLLLPSFGIQDLLSLSIRSFL 166
           F  +  L  DM+YRYSRHLLLP FG++    LS  S L
Sbjct: 82  FAPDGSLPADMIYRYSRHLLLPDFGVEGQRKLSQSSIL 119

BLAST of Spg030409 vs. ExPASy TrEMBL
Match: A0A6J1E0P9 (Adenylyltransferase and sulfurtransferase MOCS3 OS=Momordica charantia OX=3673 GN=LOC111025340 PE=3 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 1.0e-29
Identity = 69/86 (80.23%), Postives = 79/86 (91.86%), Query Frame = 0

Query: 69  MRSSTTDSSRILQEIETLKSTKSDLERRISALESQLRDLNRHQKDGLSNGSCTAVSTLES 128
           M  ++T+SSRILQ+IETLKS KSDLERRISALESQLRDLN HQ DG+SNGSCT++ST+ES
Sbjct: 1   MGLTSTESSRILQDIETLKSAKSDLERRISALESQLRDLNHHQNDGVSNGSCTSLSTVES 60

Query: 129 AFPHELSPDMMYRYSRHLLLPSFGIQ 155
            FPH LSPDM+YRYSRHLLLPSFG+Q
Sbjct: 61  GFPHGLSPDMIYRYSRHLLLPSFGVQ 86

BLAST of Spg030409 vs. ExPASy TrEMBL
Match: A0A6J1L1Q9 (Adenylyltransferase and sulfurtransferase MOCS3 OS=Cucurbita maxima OX=3661 GN=LOC111500309 PE=3 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 6.9e-26
Identity = 63/86 (73.26%), Postives = 75/86 (87.21%), Query Frame = 0

Query: 69  MRSSTTDSSRILQEIETLKSTKSDLERRISALESQLRDLNRHQKDGLSNGSCTAVSTLES 128
           M S+TT+SSRIL EIETLKS +SDL+RRIS+LESQLRDLN HQ +G+S+    +VSTL+S
Sbjct: 1   MASTTTESSRILHEIETLKSARSDLDRRISSLESQLRDLNHHQNNGVSHAPSASVSTLDS 60

Query: 129 AFPHELSPDMMYRYSRHLLLPSFGIQ 155
            FPH LSPDM+YRYSRHLLLPSFG+Q
Sbjct: 61  TFPHGLSPDMIYRYSRHLLLPSFGVQ 86

BLAST of Spg030409 vs. ExPASy TrEMBL
Match: A0A6J1F1S4 (Adenylyltransferase and sulfurtransferase MOCS3 OS=Cucurbita moschata OX=3662 GN=LOC111438631 PE=3 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 1.5e-25
Identity = 62/86 (72.09%), Postives = 75/86 (87.21%), Query Frame = 0

Query: 69  MRSSTTDSSRILQEIETLKSTKSDLERRISALESQLRDLNRHQKDGLSNGSCTAVSTLES 128
           M S+TT+SSRIL EIETLKS +SDL+RRIS+LESQLRDLN HQ +G+S+    +VSTL+S
Sbjct: 1   MASTTTESSRILHEIETLKSARSDLDRRISSLESQLRDLNHHQNNGVSHAPSASVSTLDS 60

Query: 129 AFPHELSPDMMYRYSRHLLLPSFGIQ 155
            FPH LSPDM+YRYSRHLLLP+FG+Q
Sbjct: 61  TFPHGLSPDMIYRYSRHLLLPTFGVQ 86

BLAST of Spg030409 vs. ExPASy TrEMBL
Match: A0A1S3BLE2 (Adenylyltransferase and sulfurtransferase MOCS3 OS=Cucumis melo OX=3656 GN=LOC103491093 PE=3 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 2.1e-22
Identity = 66/97 (68.04%), Postives = 75/97 (77.32%), Query Frame = 0

Query: 69  MRSSTTDSSRILQEIETLKSTKSDLERRISALESQLRDLNRHQKDGLSNGSCTAVSTLES 128
           M S+T +SSRIL EIETLKS KSDLERRISALESQL +LN+   +G+SN S T+ ST   
Sbjct: 1   MASTTGESSRILHEIETLKSAKSDLERRISALESQLHNLNQPHNNGVSNASSTSPST--- 60

Query: 129 AFPHELSPDMMYRYSRHLLLPSFGIQDLLSLSIRSFL 166
            FPH LSPDM+YRYSRHLLLPSFG+Q  L LS  S L
Sbjct: 61  -FPHGLSPDMIYRYSRHLLLPSFGVQGQLRLSKSSVL 93

BLAST of Spg030409 vs. ExPASy TrEMBL
Match: A0A0A0L690 (Adenylyltransferase and sulfurtransferase MOCS3 OS=Cucumis sativus OX=3659 GN=MOCS3 PE=3 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 4.6e-22
Identity = 65/97 (67.01%), Postives = 74/97 (76.29%), Query Frame = 0

Query: 69  MRSSTTDSSRILQEIETLKSTKSDLERRISALESQLRDLNRHQKDGLSNGSCTAVSTLES 128
           M S+T +SSRIL EIETLKS K DLERRISALESQL +LN+   +G+SN S T+ ST   
Sbjct: 1   MASTTDESSRILHEIETLKSAKFDLERRISALESQLHNLNQPHNNGVSNASSTSPST--- 60

Query: 129 AFPHELSPDMMYRYSRHLLLPSFGIQDLLSLSIRSFL 166
            FPH LSPDM+YRYSRHLLLPSFG+Q  L LS  S L
Sbjct: 61  -FPHALSPDMIYRYSRHLLLPSFGVQGQLRLSKSSIL 93

BLAST of Spg030409 vs. TAIR 10
Match: AT5G55130.2 (co-factor for nitrate, reductase and xanthine dehydrogenase 5 )

HSP 1 Score: 65.5 bits (158), Expect = 4.7e-11
Identity = 42/88 (47.73%), Postives = 57/88 (64.77%), Query Frame = 0

Query: 69  MRSSTTDSSRILQEIETLKSTKSDLERRISALESQLRDLNRHQ-KDGLSNG-SCTAVSTL 128
           M S+  DSS I++E+E LK  K+++E RIS LE++L+D    +  D +SNG S      L
Sbjct: 1   MMSNGGDSSEIVRELEELKLKKAEIEHRISTLEAKLQDTAAVELYDAVSNGDSYLTAPEL 60

Query: 129 ESAFPHELSPDMMYRYSRHLLLPSFGIQ 155
           E    H LSPD +YRYSR LLLPSF ++
Sbjct: 61  E----HGLSPDQIYRYSRQLLLPSFAVE 84

BLAST of Spg030409 vs. TAIR 10
Match: AT5G55130.1 (co-factor for nitrate, reductase and xanthine dehydrogenase 5 )

HSP 1 Score: 64.7 bits (156), Expect = 8.1e-11
Identity = 45/99 (45.45%), Postives = 61/99 (61.62%), Query Frame = 0

Query: 69  MRSSTTDSSRILQEIETLKSTKSDLERRISALESQLRDLNRHQ-KDGLSNG-SCTAVSTL 128
           M S+  DSS I++E+E LK  K+++E RIS LE++L+D    +  D +SNG S      L
Sbjct: 1   MMSNGGDSSEIVRELEELKLKKAEIEHRISTLEAKLQDTAAVELYDAVSNGDSYLTAPEL 60

Query: 129 ESAFPHELSPDMMYRYSRHLLLPSFGIQDLLSLSIRSFL 166
           E    H LSPD +YRYSR LLLPSF ++   +L   S L
Sbjct: 61  E----HGLSPDQIYRYSRQLLLPSFAVEGQSNLLKSSVL 95

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022158874.12.1e-2980.23adenylyltransferase and sulfurtransferase MOCS3 [Momordica charantia][more]
XP_023007816.11.4e-2573.26adenylyltransferase and sulfurtransferase MOCS3 [Cucurbita maxima][more]
XP_022932280.13.2e-2572.09adenylyltransferase and sulfurtransferase MOCS3 [Cucurbita moschata][more]
KAG7015532.13.2e-2572.09Adenylyltransferase and sulfurtransferase MOCS3 [Cucurbita argyrosperma subsp. a... [more]
XP_023553239.17.1e-2570.93adenylyltransferase and sulfurtransferase MOCS3 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9ZNW01.1e-0945.45Adenylyltransferase and sulfurtransferase MOCS3 OS=Arabidopsis thaliana OX=3702 ... [more]
B6TNK63.8e-0534.69Adenylyltransferase and sulfurtransferase MOCS3-1 OS=Zea mays OX=4577 GN=MOCS3-1... [more]
B4FAT01.4e-0435.71Adenylyltransferase and sulfurtransferase MOCS3-2 OS=Zea mays OX=4577 GN=MOCS3-2... [more]
Match NameE-valueIdentityDescription
A0A6J1E0P91.0e-2980.23Adenylyltransferase and sulfurtransferase MOCS3 OS=Momordica charantia OX=3673 G... [more]
A0A6J1L1Q96.9e-2673.26Adenylyltransferase and sulfurtransferase MOCS3 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1F1S41.5e-2572.09Adenylyltransferase and sulfurtransferase MOCS3 OS=Cucurbita moschata OX=3662 GN... [more]
A0A1S3BLE22.1e-2268.04Adenylyltransferase and sulfurtransferase MOCS3 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A0A0L6904.6e-2267.01Adenylyltransferase and sulfurtransferase MOCS3 OS=Cucumis sativus OX=3659 GN=MO... [more]
Match NameE-valueIdentityDescription
AT5G55130.24.7e-1147.73co-factor for nitrate, reductase and xanthine dehydrogenase 5 [more]
AT5G55130.18.1e-1145.45co-factor for nitrate, reductase and xanthine dehydrogenase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 76..110

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg030409.1Spg030409.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006777 Mo-molybdopterin cofactor biosynthetic process
biological_process GO:0002143 tRNA wobble position uridine thiolation
cellular_component GO:0005829 cytosol
molecular_function GO:0005524 ATP binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0061605 molybdopterin-synthase adenylyltransferase activity
molecular_function GO:0061604 molybdopterin-synthase sulfurtransferase activity
molecular_function GO:0004792 thiosulfate sulfurtransferase activity
molecular_function GO:0042292 URM1 activating enzyme activity