Spg030221 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg030221 vs. NCBI nr
Match: XP_022136370.1 (protein ROOT PRIMORDIUM DEFECTIVE 1 [Momordica charantia])

HSP 1 Score: 699.9 bits (1805), Expect = 1.5e-197
Identity = 336/381 (88.19%), Postives = 360/381 (94.49%), Query Frame = 0

Query: 102 TVRLPYRLSQTRAFVDAKVKWVRDPYLDFAVQREKNLRQVISLKNLIILSPFKSLPLSSV 161
           + R+PYRLSQTR FVDAKVKWVRDPYLDFAVQREKNLRQVISLKNLI+ +P KSLPLSSV
Sbjct: 5   STRIPYRLSQTRGFVDAKVKWVRDPYLDFAVQREKNLRQVISLKNLIVSNPSKSLPLSSV 64

Query: 162 SLLKQNLKVPTTTISKFFQLYPSVFIQFQPNLGLHPHVRLTPQALALHKEESTIHNSQPH 221
           SLLKQNLKVPTTT+SKFFQLYPSVF QFQP+ GLHP V+LTPQAL LHKEESTIHNSQPH
Sbjct: 65  SLLKQNLKVPTTTVSKFFQLYPSVFTQFQPSPGLHPLVKLTPQALTLHKEESTIHNSQPH 124

Query: 222 RDDVVKRLAKLLMLTGARKLPLYVVERLQWDLGLPYKFIPTLLADYPEYFQVCSMRDCST 281
           RDDVVKRLAKLLMLTGA +LPLYV+ERLQWDLGLPYKFIPTLLADYPEYFQVC++RDCST
Sbjct: 125 RDDVVKRLAKLLMLTGAGRLPLYVIERLQWDLGLPYKFIPTLLADYPEYFQVCTVRDCST 184

Query: 282 GEQTLAFELLSWRKNLSVSELKKRECLEGNGWSKNGNHIAFPMSFPRGFDLEKKVLNWVE 341
           GEQTLA E+LSWRK LS+SELK+REC +GN WS+ GNHIAFPMS P+GFD EKKV+NWVE
Sbjct: 185 GEQTLALEILSWRKELSISELKRRECPDGNVWSRKGNHIAFPMSLPQGFDFEKKVMNWVE 244

Query: 342 EWQRLPYISPYENAFHLAPNSDQAEKWAVAVLHELLCLMVSKKTEKENIMCLGEYLGFGS 401
           EWQRLPYISPYENAFHLAPNSDQAEKW VAVLHELL L+VSKKTEKENI CLGEYLGFGS
Sbjct: 245 EWQRLPYISPYENAFHLAPNSDQAEKWTVAVLHELLYLLVSKKTEKENIFCLGEYLGFGS 304

Query: 402 RFKKALVHHPGIFYVSNKIRTQTVVLREAYKKDFLVEKHPLMGMRHQYIHLMNKVVRKPT 461
           RFKKALVHHPGIFYVSNKIRTQTVVLREAY+KDFLVEKHPLMGMRHQYIHLMNKVVR+P 
Sbjct: 305 RFKKALVHHPGIFYVSNKIRTQTVVLREAYRKDFLVEKHPLMGMRHQYIHLMNKVVRRPR 364

Query: 462 PGIILASSRGKRQNNSFANKQ 483
           PGIILASSRGKRQNN ++NK+
Sbjct: 365 PGIILASSRGKRQNNPYSNKE 385

BLAST of Spg030221 vs. NCBI nr
Match: XP_022994938.1 (protein WHAT'S THIS FACTOR 1 [Cucurbita maxima])

HSP 1 Score: 693.3 bits (1788), Expect = 1.4e-195
Identity = 336/379 (88.65%), Postives = 355/379 (93.67%), Query Frame = 0

Query: 104 RLPYRLSQTRAFVDAKVKWVRDPYLDFAVQREKNLRQVISLKNLIILSPFKSLPLSSVSL 163
           R P+RL QTRAFVDAKVKWVRDPYLDF VQREKNLRQVISLKNLI+ SPFKS+PLSSVSL
Sbjct: 7   RAPHRLCQTRAFVDAKVKWVRDPYLDFVVQREKNLRQVISLKNLIVSSPFKSVPLSSVSL 66

Query: 164 LKQNLKVPTTTISKFFQLYPSVFIQFQPNLGLHPHVRLTPQALALHKEESTIHNSQPHRD 223
           ++QNLK PTT+ISKFFQLYPSVFIQFQP+LGLHPHV+LT Q+LALHKEE TIHNSQPHRD
Sbjct: 67  MRQNLKAPTTSISKFFQLYPSVFIQFQPSLGLHPHVKLTSQSLALHKEELTIHNSQPHRD 126

Query: 224 DVVKRLAKLLMLTGARKLPLYVVERLQWDLGLPYKFIPTLLADYPEYFQVCSMRDCSTGE 283
           D+VKRLAKLLMLTGA KLPLYV+E+LQWDLGLPYKFIP LLADYPEYFQVCSMRDCSTGE
Sbjct: 127 DIVKRLAKLLMLTGAGKLPLYVIEKLQWDLGLPYKFIPALLADYPEYFQVCSMRDCSTGE 186

Query: 284 QTLAFELLSWRKNLSVSELKKRECLEGNGWSKNGNHIAFPMSFPRGFDLEKKVLNWVEEW 343
           QTLA ELLSWR++LSVSELKKR  LEGNGW +NGNHIAFPMSFPRGFDLEKKVLNWVEEW
Sbjct: 187 QTLALELLSWREDLSVSELKKRHSLEGNGWRRNGNHIAFPMSFPRGFDLEKKVLNWVEEW 246

Query: 344 QRLPYISPYENAFHLAPNSDQAEKWAVAVLHELLCLMVSKKTEKENIMCLGEYLGFGSRF 403
           QRLPYISPYENAFHLAPNSDQAEKW  AVLHELL LMVSKKTEKENI CLGEYLGFGSRF
Sbjct: 247 QRLPYISPYENAFHLAPNSDQAEKWTTAVLHELLYLMVSKKTEKENIFCLGEYLGFGSRF 306

Query: 404 KKALVHHPGIFYVSNKIRTQTVVLREAYKKDFLVEKHPLMGMRHQYIHLMNKVVRKPTPG 463
           KKA+VHHPGIFYVSNKIRTQTVVLREAY K+FLVEKH LMGMRHQYIHLMNK VR+P P 
Sbjct: 307 KKAIVHHPGIFYVSNKIRTQTVVLREAYNKNFLVEKHLLMGMRHQYIHLMNKAVRRPRPR 366

Query: 464 IILASSRGKRQNNSFANKQ 483
           IILASSRGKRQNN  ANK+
Sbjct: 367 IILASSRGKRQNNPSANKE 385

BLAST of Spg030221 vs. NCBI nr
Match: KAG7031364.1 (Protein ROOT PRIMORDIUM DEFECTIVE 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 690.6 bits (1781), Expect = 9.3e-195
Identity = 337/379 (88.92%), Postives = 355/379 (93.67%), Query Frame = 0

Query: 104 RLPYRLSQTRAFVDAKVKWVRDPYLDFAVQREKNLRQVISLKNLIILSPFKSLPLSSVSL 163
           R P+RL QTRAFVDAKVKWVRDPYLDF VQREKNLRQVISLKNLI+ SPFKS+PLSSVSL
Sbjct: 7   RAPHRLCQTRAFVDAKVKWVRDPYLDFVVQREKNLRQVISLKNLIVSSPFKSVPLSSVSL 66

Query: 164 LKQNLKVPTTTISKFFQLYPSVFIQFQPNLGLHPHVRLTPQALALHKEESTIHNSQPHRD 223
           ++QNLKVPTT+ISKF QLYPSVFIQFQP+LGLHPHV+LT QALALHKEESTIHNSQPHRD
Sbjct: 67  MRQNLKVPTTSISKFLQLYPSVFIQFQPSLGLHPHVKLTSQALALHKEESTIHNSQPHRD 126

Query: 224 DVVKRLAKLLMLTGARKLPLYVVERLQWDLGLPYKFIPTLLADYPEYFQVCSMRDCSTGE 283
           DVVKRLAKLLMLTGA KLPLYV+E+LQWDLGLPYKFIPTLLADYPEYFQVCS+RDCSTGE
Sbjct: 127 DVVKRLAKLLMLTGAGKLPLYVIEKLQWDLGLPYKFIPTLLADYPEYFQVCSVRDCSTGE 186

Query: 284 QTLAFELLSWRKNLSVSELKKRECLEGNGWSKNGNHIAFPMSFPRGFDLEKKVLNWVEEW 343
           QTLA ELLSWR++LSVSELKKR  LEGNGW +NG HIAFPMSFPRGFDLEKKVLNWVEEW
Sbjct: 187 QTLALELLSWREDLSVSELKKRHSLEGNGWRRNGIHIAFPMSFPRGFDLEKKVLNWVEEW 246

Query: 344 QRLPYISPYENAFHLAPNSDQAEKWAVAVLHELLCLMVSKKTEKENIMCLGEYLGFGSRF 403
           QRLPYISPYENAFHLAPNSDQAEKW  AVLHELL LMVSKKTEKENI CLGEYLGFGSRF
Sbjct: 247 QRLPYISPYENAFHLAPNSDQAEKWTTAVLHELLYLMVSKKTEKENIFCLGEYLGFGSRF 306

Query: 404 KKALVHHPGIFYVSNKIRTQTVVLREAYKKDFLVEKHPLMGMRHQYIHLMNKVVRKPTPG 463
           KKA+VHHPGIFYVSNKIRTQTVVLREAY K+FLVEKH LMGMRHQYIHLMNK VR+P P 
Sbjct: 307 KKAIVHHPGIFYVSNKIRTQTVVLREAYNKNFLVEKHLLMGMRHQYIHLMNKAVRRPRPR 366

Query: 464 IILASSRGKRQNNSFANKQ 483
           IILASSR KRQNN  ANK+
Sbjct: 367 IILASSRDKRQNNPSANKE 385

BLAST of Spg030221 vs. NCBI nr
Match: XP_023536124.1 (protein WHAT'S THIS FACTOR 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 686.0 bits (1769), Expect = 2.3e-193
Identity = 334/379 (88.13%), Postives = 354/379 (93.40%), Query Frame = 0

Query: 104 RLPYRLSQTRAFVDAKVKWVRDPYLDFAVQREKNLRQVISLKNLIILSPFKSLPLSSVSL 163
           R P+RL QTRAFVDAKVKWVRDPYLDF VQREKNLRQVISLKNLI+ SPFKS+PLSSVSL
Sbjct: 7   RAPHRLCQTRAFVDAKVKWVRDPYLDFVVQREKNLRQVISLKNLIVSSPFKSVPLSSVSL 66

Query: 164 LKQNLKVPTTTISKFFQLYPSVFIQFQPNLGLHPHVRLTPQALALHKEESTIHNSQPHRD 223
           ++QNLKVPTT+ISKF QLYPSVFIQFQP+LGLHPHV++T QALALHKEES IHNSQPHRD
Sbjct: 67  MRQNLKVPTTSISKFLQLYPSVFIQFQPSLGLHPHVKITSQALALHKEESAIHNSQPHRD 126

Query: 224 DVVKRLAKLLMLTGARKLPLYVVERLQWDLGLPYKFIPTLLADYPEYFQVCSMRDCSTGE 283
           DVVKRLAKLLMLTGA KLPLYV+E+LQWDLGLPYKFIP LLADYPEYFQVCS+RD STGE
Sbjct: 127 DVVKRLAKLLMLTGAGKLPLYVIEKLQWDLGLPYKFIPALLADYPEYFQVCSVRDRSTGE 186

Query: 284 QTLAFELLSWRKNLSVSELKKRECLEGNGWSKNGNHIAFPMSFPRGFDLEKKVLNWVEEW 343
           QTLA ELLSWR++LSVSELKKR  LEGNGW +NGNHIAFPMSFPRGFDLEKKVLNWVEEW
Sbjct: 187 QTLALELLSWREDLSVSELKKRHSLEGNGWRRNGNHIAFPMSFPRGFDLEKKVLNWVEEW 246

Query: 344 QRLPYISPYENAFHLAPNSDQAEKWAVAVLHELLCLMVSKKTEKENIMCLGEYLGFGSRF 403
           QRLPYISPYENAFHLAPNSDQAEKW  AVLHELL LMVSKKTEKENI CLGEYLGFGSRF
Sbjct: 247 QRLPYISPYENAFHLAPNSDQAEKWTTAVLHELLYLMVSKKTEKENIFCLGEYLGFGSRF 306

Query: 404 KKALVHHPGIFYVSNKIRTQTVVLREAYKKDFLVEKHPLMGMRHQYIHLMNKVVRKPTPG 463
           KKA+VHHPGIFYVSNKIRTQTVVLREAY K+FLVEKH LMGMRHQYIHLMN+ VR+P P 
Sbjct: 307 KKAIVHHPGIFYVSNKIRTQTVVLREAYNKNFLVEKHLLMGMRHQYIHLMNQAVRRPRPR 366

Query: 464 IILASSRGKRQNNSFANKQ 483
           IILASSRGKRQNN  ANK+
Sbjct: 367 IILASSRGKRQNNPSANKE 385

BLAST of Spg030221 vs. NCBI nr
Match: XP_022941952.1 (protein WHAT'S THIS FACTOR 1 [Cucurbita moschata])

HSP 1 Score: 685.6 bits (1768), Expect = 3.0e-193
Identity = 335/379 (88.39%), Postives = 354/379 (93.40%), Query Frame = 0

Query: 104 RLPYRLSQTRAFVDAKVKWVRDPYLDFAVQREKNLRQVISLKNLIILSPFKSLPLSSVSL 163
           R P+RL QTRAFVDAKVKWVRDPYLDF VQRE+NLRQVISLKNLI+ SPFKS+PLSSVSL
Sbjct: 7   RAPHRLCQTRAFVDAKVKWVRDPYLDFVVQREENLRQVISLKNLIVSSPFKSVPLSSVSL 66

Query: 164 LKQNLKVPTTTISKFFQLYPSVFIQFQPNLGLHPHVRLTPQALALHKEESTIHNSQPHRD 223
           ++QNLKVPTT+ISKF QLYPSVFIQFQP+LGLHPHV+LT QALALHKEESTIHNSQPHRD
Sbjct: 67  MRQNLKVPTTSISKFLQLYPSVFIQFQPSLGLHPHVKLTSQALALHKEESTIHNSQPHRD 126

Query: 224 DVVKRLAKLLMLTGARKLPLYVVERLQWDLGLPYKFIPTLLADYPEYFQVCSMRDCSTGE 283
           DVVKRLAKLLMLTGA KLPLYV+E+LQWDLGLPYKFIPTLLADYPEYFQVCS+RDCSTGE
Sbjct: 127 DVVKRLAKLLMLTGAGKLPLYVIEKLQWDLGLPYKFIPTLLADYPEYFQVCSVRDCSTGE 186

Query: 284 QTLAFELLSWRKNLSVSELKKRECLEGNGWSKNGNHIAFPMSFPRGFDLEKKVLNWVEEW 343
           QTLA ELLSWR++LSVSELKKR  LEGNGW +NG HIAFPMSFPRGFDLEKKVLNWVEEW
Sbjct: 187 QTLALELLSWREDLSVSELKKRHSLEGNGWRRNGIHIAFPMSFPRGFDLEKKVLNWVEEW 246

Query: 344 QRLPYISPYENAFHLAPNSDQAEKWAVAVLHELLCLMVSKKTEKENIMCLGEYLGFGSRF 403
           QRLPYISPYENAFHLAPNSDQAEKW  AVLHELL LMVSKKTEKENI CLGEYLGFGSRF
Sbjct: 247 QRLPYISPYENAFHLAPNSDQAEKWTTAVLHELLYLMVSKKTEKENISCLGEYLGFGSRF 306

Query: 404 KKALVHHPGIFYVSNKIRTQTVVLREAYKKDFLVEKHPLMGMRHQYIHLMNKVVRKPTPG 463
           KKA+VHHPGIFYVSNKIRTQTVVLREAY K+FLVEKH LMGMRHQYI LMNK VR+P P 
Sbjct: 307 KKAIVHHPGIFYVSNKIRTQTVVLREAYNKNFLVEKHLLMGMRHQYIRLMNKAVRRPRPR 366

Query: 464 IILASSRGKRQNNSFANKQ 483
           IILASSR KRQNN  ANK+
Sbjct: 367 IILASSRDKRQNNPSANKE 385

BLAST of Spg030221 vs. ExPASy Swiss-Prot
Match: Q9ZUZ6 (Protein WHAT'S THIS FACTOR 9, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=WTF9 PE=4 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 6.8e-55
Identity = 131/348 (37.64%), Postives = 199/348 (57.18%), Query Frame = 0

Query: 109 LSQTRAFVDAKVKWVRDPYLDFA--VQREKNLRQVISLKNLIILSPFKSLPLSSVSLLKQ 168
           L+Q R +VD  +KW RDPY D    + R   L+ V+SLKN I+  P + +P+S++S   +
Sbjct: 18  LTQKRTYVDVYMKWKRDPYFDNIEHILRSSQLKSVVSLKNCIVQEPNRCIPISAISKKTR 77

Query: 169 NLKVPTTTISKFFQLYPSVFIQF-QPNLGLHPHVRLTPQALALHKEESTIHNSQPHRDDV 228
              V +T I+ F + +PS+F +F  P   L P  RLTP+A  L ++E  ++  Q   DD+
Sbjct: 78  QFDV-STKIAHFLRKFPSIFEEFVGPEYNL-PWFRLTPEATELDRQERVVY--QTSADDL 137

Query: 229 VKRLAKLLMLTGARKLPLYVVERLQWDLGLPYKFIPTLLADYPEYFQVCSMRDCSTGEQT 288
             RL KL++++    LPL +V+ ++W LGLP  ++     +    F+   M D   G + 
Sbjct: 138 RDRLKKLILMSKDNVLPLSIVQGMKWYLGLPDDYLQFPDMNLDSSFRFVDMED---GVKG 197

Query: 289 LAFELLSWRKNLSVSELKKRECLEGNGWSKNGNHIAFPMSFPRGFDLEKKVLNWVEEWQR 348
           LA +     K LSV  L+K    +  G   +   I FP+   +G  L  K+ +W+ E+Q+
Sbjct: 198 LAVDYNGGDKVLSV--LQKNAMKKRRG-EVSLEEIEFPLFPSKGCRLRVKIEDWLMEFQK 257

Query: 349 LPYISPYENAFHLAPNSDQAEKWAVAVLHELLCLMVSKKTEKENIMCLGEYLGFGSRFKK 408
           LPY+SPY++   L P+SD AEK  V  LHELLCL V    E++ ++CL ++ G   +  K
Sbjct: 258 LPYVSPYDDYSCLDPSSDIAEKRVVGFLHELLCLFVEHSAERKKLLCLKKHFGLPQKVHK 317

Query: 409 ALVHHPGIFYVSNKIRTQTVVLREAYKKDFLVEKHPLMGMRHQYIHLM 454
           A   HP IFY+S K +T T +LRE Y+    VE HP++G+R +YI LM
Sbjct: 318 AFERHPQIFYLSMKNKTCTAILREPYRDKASVETHPVLGVRKKYIQLM 355

BLAST of Spg030221 vs. ExPASy Swiss-Prot
Match: B6TTV8 (Protein WHAT'S THIS FACTOR 1, chloroplastic OS=Zea mays OX=4577 GN=WTF1 PE=1 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 4.6e-35
Identity = 115/386 (29.79%), Postives = 188/386 (48.70%), Query Frame = 0

Query: 106 PYRLSQTRAFV--DAKVKWVRDPYLDFAVQREKNLRQVISLKNLIILSPFKSLPLSSVSL 165
           P RL    A V   A VK  ++   D  +QR+K L+ V+ L+N+++  P + + L  +  
Sbjct: 39  PLRLRPRPAAVVAQAAVKRRKEAPFDTVIQRDKKLKLVLKLRNILVAQPDRVMSLRELGR 98

Query: 166 LKQNLKVP-TTTISKFFQLYPSVFIQFQPNLGLHPHVRLTPQALALHKEESTIHNSQPHR 225
            +++L +     +    + +P VF   +  +      RLTP A  L+ +E  + N     
Sbjct: 99  FRRDLGLTRKRRLIALLRRFPGVFDVVEEGV-YSLRFRLTPAAERLYLDELRLRNES--E 158

Query: 226 DDVVKRLAKLLMLTGARKLPLYVVERLQWDLGLPYKFIPTLLADYPEYFQVCSMRDCSTG 285
              V +L KLLM++  +++ +  V  L+ DLGLP +F  T+   YP+YF+V  M      
Sbjct: 159 GLAVAKLRKLLMMSQEKRILIEKVAHLKHDLGLPPEFRDTVCLRYPQYFRVVRM------ 218

Query: 286 EQTLAFELLSWRKNLSVS--ELKKRECLEGNGWSKNGNHIAFPMSF-----PRGFDLEKK 345
           ++  A EL  W   L+VS  EL + E        +N   I  P+ F     P+G  L + 
Sbjct: 219 DRGPALELTHWDPELAVSAAELAEEESRAREAEERN-LIIDRPLKFNRVRLPKGLKLTRG 278

Query: 346 VLNWVEEWQRLPYISPYENAFHLAPNSDQAEKWAVAVLHELLCLMVSKKTEKENIMCLGE 405
               +  ++ +PYISPY +  HL   SD+ EK A  V+HE+L L V K+T  +++    E
Sbjct: 279 EARRIARFKEMPYISPYADFSHLRSGSDEKEKHACGVVHEILSLTVEKRTLVDHLTHFRE 338

Query: 406 YLGFGSRFKKALVHHPGIFYVSNKIRTQTVVLREAYKKDFLVEKHPLMGMRHQYIHLMNK 465
              F    +  ++ HP +FYVS K    +V LREAYK   LVEK+ L+ ++ + +  +  
Sbjct: 339 EFRFSQSLRGMIIRHPDMFYVSFKGDRDSVFLREAYKDSQLVEKNQLVLLKEK-MRALVA 398

Query: 466 VVRKPTPGIILASSRGKRQNNSFANK 482
           V R P    +      +  N S  ++
Sbjct: 399 VPRFPRRAAVGTGEEAEGMNGSLQSR 413

BLAST of Spg030221 vs. ExPASy Swiss-Prot
Match: Q65XL5 (Protein WHAT'S THIS FACTOR 1 homolog, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0571100 PE=2 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 3.3e-33
Identity = 107/368 (29.08%), Postives = 181/368 (49.18%), Query Frame = 0

Query: 118 AKVKWVRDPYLDFAVQREKNLRQVISLKNLIILSPFKSLPLSSVSLLKQNLKVP-TTTIS 177
           A VK  ++   D  +QR+K L+ V+ L+N+++ +P + + L  +   +++L +     + 
Sbjct: 59  AAVKRRKEIPFDNVIQRDKKLKLVLKLRNILVSNPDRVMSLRDLGRFRRDLGLTRKRRLI 118

Query: 178 KFFQLYPSVFIQFQPNLGLHPHVRLTPQALALHKEESTIHNSQPHRDDVVKRLAKLLMLT 237
              + +P VF   +  +      RLTP A  L+ +E  +H         V +L KLLM++
Sbjct: 119 ALLKRFPGVFEVVEEGV-YSLRFRLTPAAERLYLDE--LHLKNESEGLAVTKLRKLLMMS 178

Query: 238 GARKLPLYVVERLQWDLGLPYKFIPTLLADYPEYFQVCSMRDCSTGEQTLAFELLSWRKN 297
             +++ +  +  L+ DLGLP +F  T+   YP+YF+V  M      ++    EL  W   
Sbjct: 179 QDKRILIEKIAHLKNDLGLPPEFRDTICLRYPQYFRVVQM------DRGPGLELTHWDPE 238

Query: 298 LSVSELKKRECLEGNGWSKNGNHIAF--PMSF-----PRGFDLEKKVLNWVEEWQRLPYI 357
           L+VS  +  E  E     +   ++    P+ F     P+G  L +     V +++ +PYI
Sbjct: 239 LAVSAAEVAE-EENRAREEQERNLIIDRPLKFNRVKLPQGLKLSRGEARRVAQFKEMPYI 298

Query: 358 SPYENAFHLAPNSDQAEKWAVAVLHELLCLMVSKKTEKENIMCLGEYLGFGSRFKKALVH 417
           SPY +  HL   S + EK A  V+HE+L L + K+T  +++    E   F    +  L+ 
Sbjct: 299 SPYSDFSHLRSGSAEKEKHACGVVHEILSLTLEKRTLVDHLTHFREEFRFSQSLRGMLIR 358

Query: 418 HPGIFYVSNKIRTQTVVLREAYKKDFLVEKHPLMGMRHQYIHLMNKVVRKPTPGIILASS 477
           HP +FYVS K    +V LREAYK   LVEK  L+ ++ + +  +  V R P  G+   S 
Sbjct: 359 HPDMFYVSLKGDRDSVFLREAYKNSQLVEKSKLVLLKEK-MRALVAVPRFPRRGVPATSE 415

BLAST of Spg030221 vs. ExPASy Swiss-Prot
Match: A0MFS5 (Protein WHAT'S THIS FACTOR 1 homolog, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g01037 PE=3 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 6.2e-32
Identity = 105/355 (29.58%), Postives = 176/355 (49.58%), Query Frame = 0

Query: 116 VDAKVKWVRDPYLDFAVQREKNLRQVISLKNLIILSPFKSLPLSSVSLLKQNLKV-PTTT 175
           V A VK  ++   D  VQR+K L+ V++++ +++  P + + L  +   +++L +     
Sbjct: 72  VRAAVKRRKELTFDSVVQRDKKLKLVLNIRKILVSQPDRMMSLRGLGKYRRDLGLKKRRR 131

Query: 176 ISKFFQLYPSVFIQFQPNLGLHPHVRLTPQALALHKEESTIHNSQPHRDDVVKRLAKLLM 235
                + YP VF +           ++T +A  L+ +E  I N     D +V +L KL+M
Sbjct: 132 FIALLRKYPGVF-EIVEEGAYSLRFKMTSEAERLYLDEMRIRNEL--EDVLVVKLRKLVM 191

Query: 236 LTGARKLPLYVVERLQWDLGLPYKFIPTLLADYPEYFQVCSMRDCSTGEQTLAFELLSWR 295
           ++  +++ L  +  L+ DLGLP +F  T+   YP+YF+V          +  A EL  W 
Sbjct: 192 MSIDKRILLEKISHLKTDLGLPLEFRDTICQRYPQYFRVVPT------PRGPALELTHWD 251

Query: 296 KNLSVSELKKRECLEGNGWSKNGNHIA-FPMSF-----PRGFDLEKKVLNWVEEWQRLPY 355
             L+VS  +  E       S+  N I   P  F     PRG +L K     + +++ + Y
Sbjct: 252 PELAVSAAELSEDDNRTRESEERNLIIDRPPKFNRVKLPRGLNLSKSETRKISQFRDMQY 311

Query: 356 ISPYENAFHLAPNSDQAEKWAVAVLHELLCLMVSKKTEKENIMCLGEYLGFGSRFKKALV 415
           ISPY++  HL   + + EK A  V+HELL L   K+T  +++    E   F  + +  L+
Sbjct: 312 ISPYKDFSHLRSGTLEKEKHACGVIHELLSLTTEKRTLVDHLTHFREEFRFSQQLRGMLI 371

Query: 416 HHPGIFYVSNKIRTQTVVLREAYKKDFLVEKHPLMGMRHQYIHLMNKVVRKPTPG 464
            HP +FYVS K    +V LREAY+   L++K PL  ++ +   L++ V R P  G
Sbjct: 372 RHPDLFYVSLKGERDSVFLREAYRNSELIDKDPLTLVKEKMRALVS-VPRFPRRG 416

BLAST of Spg030221 vs. ExPASy Swiss-Prot
Match: Q689D6 (Protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Arabidopsis thaliana OX=3702 GN=RPD1 PE=1 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 1.2e-27
Identity = 109/368 (29.62%), Postives = 176/368 (47.83%), Query Frame = 0

Query: 106 PYRLSQTRAFVDAKVKWVRDPYLDFAVQREKNLRQVISLKNLIILSPFKSLPLSSVSLLK 165
           P+  S T   +  K   VRD   D  ++ EK +R+V+   +LI+  P  ++ +S +  L 
Sbjct: 33  PFSQSTT---IPKKQDRVRDHGYDNYMEVEKKIRKVVKFHSLILSQPNHTIAISLLDTLA 92

Query: 166 QNLKV--PTTTISKFFQLYPSVFIQFQPNLGLHPHVRLTPQAL-ALHKEESTIHNSQPHR 225
           + L +         F   +P VF  ++  +    + RLT +AL  +  E   + +  P  
Sbjct: 93  RRLGLGFKQHEPGAFLLKFPHVFEIYEHPVQRILYCRLTRKALDQIRHEHEAVLDQIP-- 152

Query: 226 DDVVKRLAKLLMLTGARKLPLYVVERLQWDLGLPYKFIPTLLADYPEYFQVCSMRDCSTG 285
            D V RL KL+M++   ++ L  V   + + GLP  F  +++  +P++F++        G
Sbjct: 153 -DAVTRLRKLVMMSNTGRIRLEHVRIARTEFGLPEDFEYSVILKHPQFFRLID------G 212

Query: 286 EQT--LAFELLSWRKNLSVSELKKRECLE--GNGWSKNGNHIAFPMSFPRGFDLEKKVLN 345
           E+T     E++    NLS+  +++   +E    G        +F ++FP GF + K    
Sbjct: 213 EETRDKYIEIVEKDPNLSICAIERVREIEYRTKGIDAEDVRFSFVVNFPPGFKIGKYFRI 272

Query: 346 WVEEWQRLPYISPYENAFHLAPNSDQA----EKWAVAVLHELLCLMVSKKTEKENIMCLG 405
            V +WQRLPY SPYE+       S +A    EK +VA +HELL L V KK   E I    
Sbjct: 273 AVWKWQRLPYWSPYEDISGYDLRSMEAQNRLEKRSVACIHELLSLTVEKKITLERIAHFR 332

Query: 406 EYLGFGSRFKKALVHHPGIFYVS---NKIRTQTVVLREAYKKDFLVEKHPLMGMRHQYIH 460
             +    + K+ L+ H GIFY+S   N  +  TV LRE YK+  LVE + +   R +   
Sbjct: 333 NVMNLPKKLKEFLLQHQGIFYISTRGNYGKLHTVFLREGYKRGELVEPNDVYLARRRLAE 388

BLAST of Spg030221 vs. ExPASy TrEMBL
Match: A0A6J1C3P8 (protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Momordica charantia OX=3673 GN=LOC111008095 PE=4 SV=1)

HSP 1 Score: 699.9 bits (1805), Expect = 7.4e-198
Identity = 336/381 (88.19%), Postives = 360/381 (94.49%), Query Frame = 0

Query: 102 TVRLPYRLSQTRAFVDAKVKWVRDPYLDFAVQREKNLRQVISLKNLIILSPFKSLPLSSV 161
           + R+PYRLSQTR FVDAKVKWVRDPYLDFAVQREKNLRQVISLKNLI+ +P KSLPLSSV
Sbjct: 5   STRIPYRLSQTRGFVDAKVKWVRDPYLDFAVQREKNLRQVISLKNLIVSNPSKSLPLSSV 64

Query: 162 SLLKQNLKVPTTTISKFFQLYPSVFIQFQPNLGLHPHVRLTPQALALHKEESTIHNSQPH 221
           SLLKQNLKVPTTT+SKFFQLYPSVF QFQP+ GLHP V+LTPQAL LHKEESTIHNSQPH
Sbjct: 65  SLLKQNLKVPTTTVSKFFQLYPSVFTQFQPSPGLHPLVKLTPQALTLHKEESTIHNSQPH 124

Query: 222 RDDVVKRLAKLLMLTGARKLPLYVVERLQWDLGLPYKFIPTLLADYPEYFQVCSMRDCST 281
           RDDVVKRLAKLLMLTGA +LPLYV+ERLQWDLGLPYKFIPTLLADYPEYFQVC++RDCST
Sbjct: 125 RDDVVKRLAKLLMLTGAGRLPLYVIERLQWDLGLPYKFIPTLLADYPEYFQVCTVRDCST 184

Query: 282 GEQTLAFELLSWRKNLSVSELKKRECLEGNGWSKNGNHIAFPMSFPRGFDLEKKVLNWVE 341
           GEQTLA E+LSWRK LS+SELK+REC +GN WS+ GNHIAFPMS P+GFD EKKV+NWVE
Sbjct: 185 GEQTLALEILSWRKELSISELKRRECPDGNVWSRKGNHIAFPMSLPQGFDFEKKVMNWVE 244

Query: 342 EWQRLPYISPYENAFHLAPNSDQAEKWAVAVLHELLCLMVSKKTEKENIMCLGEYLGFGS 401
           EWQRLPYISPYENAFHLAPNSDQAEKW VAVLHELL L+VSKKTEKENI CLGEYLGFGS
Sbjct: 245 EWQRLPYISPYENAFHLAPNSDQAEKWTVAVLHELLYLLVSKKTEKENIFCLGEYLGFGS 304

Query: 402 RFKKALVHHPGIFYVSNKIRTQTVVLREAYKKDFLVEKHPLMGMRHQYIHLMNKVVRKPT 461
           RFKKALVHHPGIFYVSNKIRTQTVVLREAY+KDFLVEKHPLMGMRHQYIHLMNKVVR+P 
Sbjct: 305 RFKKALVHHPGIFYVSNKIRTQTVVLREAYRKDFLVEKHPLMGMRHQYIHLMNKVVRRPR 364

Query: 462 PGIILASSRGKRQNNSFANKQ 483
           PGIILASSRGKRQNN ++NK+
Sbjct: 365 PGIILASSRGKRQNNPYSNKE 385

BLAST of Spg030221 vs. ExPASy TrEMBL
Match: A0A6J1K6G9 (protein WHAT'S THIS FACTOR 1 OS=Cucurbita maxima OX=3661 GN=LOC111490493 PE=4 SV=1)

HSP 1 Score: 693.3 bits (1788), Expect = 7.0e-196
Identity = 336/379 (88.65%), Postives = 355/379 (93.67%), Query Frame = 0

Query: 104 RLPYRLSQTRAFVDAKVKWVRDPYLDFAVQREKNLRQVISLKNLIILSPFKSLPLSSVSL 163
           R P+RL QTRAFVDAKVKWVRDPYLDF VQREKNLRQVISLKNLI+ SPFKS+PLSSVSL
Sbjct: 7   RAPHRLCQTRAFVDAKVKWVRDPYLDFVVQREKNLRQVISLKNLIVSSPFKSVPLSSVSL 66

Query: 164 LKQNLKVPTTTISKFFQLYPSVFIQFQPNLGLHPHVRLTPQALALHKEESTIHNSQPHRD 223
           ++QNLK PTT+ISKFFQLYPSVFIQFQP+LGLHPHV+LT Q+LALHKEE TIHNSQPHRD
Sbjct: 67  MRQNLKAPTTSISKFFQLYPSVFIQFQPSLGLHPHVKLTSQSLALHKEELTIHNSQPHRD 126

Query: 224 DVVKRLAKLLMLTGARKLPLYVVERLQWDLGLPYKFIPTLLADYPEYFQVCSMRDCSTGE 283
           D+VKRLAKLLMLTGA KLPLYV+E+LQWDLGLPYKFIP LLADYPEYFQVCSMRDCSTGE
Sbjct: 127 DIVKRLAKLLMLTGAGKLPLYVIEKLQWDLGLPYKFIPALLADYPEYFQVCSMRDCSTGE 186

Query: 284 QTLAFELLSWRKNLSVSELKKRECLEGNGWSKNGNHIAFPMSFPRGFDLEKKVLNWVEEW 343
           QTLA ELLSWR++LSVSELKKR  LEGNGW +NGNHIAFPMSFPRGFDLEKKVLNWVEEW
Sbjct: 187 QTLALELLSWREDLSVSELKKRHSLEGNGWRRNGNHIAFPMSFPRGFDLEKKVLNWVEEW 246

Query: 344 QRLPYISPYENAFHLAPNSDQAEKWAVAVLHELLCLMVSKKTEKENIMCLGEYLGFGSRF 403
           QRLPYISPYENAFHLAPNSDQAEKW  AVLHELL LMVSKKTEKENI CLGEYLGFGSRF
Sbjct: 247 QRLPYISPYENAFHLAPNSDQAEKWTTAVLHELLYLMVSKKTEKENIFCLGEYLGFGSRF 306

Query: 404 KKALVHHPGIFYVSNKIRTQTVVLREAYKKDFLVEKHPLMGMRHQYIHLMNKVVRKPTPG 463
           KKA+VHHPGIFYVSNKIRTQTVVLREAY K+FLVEKH LMGMRHQYIHLMNK VR+P P 
Sbjct: 307 KKAIVHHPGIFYVSNKIRTQTVVLREAYNKNFLVEKHLLMGMRHQYIHLMNKAVRRPRPR 366

Query: 464 IILASSRGKRQNNSFANKQ 483
           IILASSRGKRQNN  ANK+
Sbjct: 367 IILASSRGKRQNNPSANKE 385

BLAST of Spg030221 vs. ExPASy TrEMBL
Match: A0A6J1FNW3 (protein WHAT'S THIS FACTOR 1 OS=Cucurbita moschata OX=3662 GN=LOC111447163 PE=4 SV=1)

HSP 1 Score: 685.6 bits (1768), Expect = 1.5e-193
Identity = 335/379 (88.39%), Postives = 354/379 (93.40%), Query Frame = 0

Query: 104 RLPYRLSQTRAFVDAKVKWVRDPYLDFAVQREKNLRQVISLKNLIILSPFKSLPLSSVSL 163
           R P+RL QTRAFVDAKVKWVRDPYLDF VQRE+NLRQVISLKNLI+ SPFKS+PLSSVSL
Sbjct: 7   RAPHRLCQTRAFVDAKVKWVRDPYLDFVVQREENLRQVISLKNLIVSSPFKSVPLSSVSL 66

Query: 164 LKQNLKVPTTTISKFFQLYPSVFIQFQPNLGLHPHVRLTPQALALHKEESTIHNSQPHRD 223
           ++QNLKVPTT+ISKF QLYPSVFIQFQP+LGLHPHV+LT QALALHKEESTIHNSQPHRD
Sbjct: 67  MRQNLKVPTTSISKFLQLYPSVFIQFQPSLGLHPHVKLTSQALALHKEESTIHNSQPHRD 126

Query: 224 DVVKRLAKLLMLTGARKLPLYVVERLQWDLGLPYKFIPTLLADYPEYFQVCSMRDCSTGE 283
           DVVKRLAKLLMLTGA KLPLYV+E+LQWDLGLPYKFIPTLLADYPEYFQVCS+RDCSTGE
Sbjct: 127 DVVKRLAKLLMLTGAGKLPLYVIEKLQWDLGLPYKFIPTLLADYPEYFQVCSVRDCSTGE 186

Query: 284 QTLAFELLSWRKNLSVSELKKRECLEGNGWSKNGNHIAFPMSFPRGFDLEKKVLNWVEEW 343
           QTLA ELLSWR++LSVSELKKR  LEGNGW +NG HIAFPMSFPRGFDLEKKVLNWVEEW
Sbjct: 187 QTLALELLSWREDLSVSELKKRHSLEGNGWRRNGIHIAFPMSFPRGFDLEKKVLNWVEEW 246

Query: 344 QRLPYISPYENAFHLAPNSDQAEKWAVAVLHELLCLMVSKKTEKENIMCLGEYLGFGSRF 403
           QRLPYISPYENAFHLAPNSDQAEKW  AVLHELL LMVSKKTEKENI CLGEYLGFGSRF
Sbjct: 247 QRLPYISPYENAFHLAPNSDQAEKWTTAVLHELLYLMVSKKTEKENISCLGEYLGFGSRF 306

Query: 404 KKALVHHPGIFYVSNKIRTQTVVLREAYKKDFLVEKHPLMGMRHQYIHLMNKVVRKPTPG 463
           KKA+VHHPGIFYVSNKIRTQTVVLREAY K+FLVEKH LMGMRHQYI LMNK VR+P P 
Sbjct: 307 KKAIVHHPGIFYVSNKIRTQTVVLREAYNKNFLVEKHLLMGMRHQYIRLMNKAVRRPRPR 366

Query: 464 IILASSRGKRQNNSFANKQ 483
           IILASSR KRQNN  ANK+
Sbjct: 367 IILASSRDKRQNNPSANKE 385

BLAST of Spg030221 vs. ExPASy TrEMBL
Match: A0A5A7T0U1 (Protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold360G00230 PE=4 SV=1)

HSP 1 Score: 674.5 bits (1739), Expect = 3.3e-190
Identity = 325/379 (85.75%), Postives = 356/379 (93.93%), Query Frame = 0

Query: 104 RLPYRLSQTRAFVDAKVKWVRDPYLDFAVQREKNLRQVISLKNLIILSPFKSLPLSSVSL 163
           R+P+R +Q RAFVDAKVKWVRDPYLDFAVQREKNL+QVISLKN+II SP  S+PLSSVSL
Sbjct: 7   RIPHRFTQIRAFVDAKVKWVRDPYLDFAVQREKNLKQVISLKNIIISSPLTSVPLSSVSL 66

Query: 164 LKQNLKVPTTTISKFFQLYPSVFIQFQPNLGLHPHVRLTPQALALHKEESTIHNSQPHRD 223
           LKQNLKVPTTTISKFF+LYPSVFIQFQP+LGLHPHV++T QAL LHKEESTIHNS+PHRD
Sbjct: 67  LKQNLKVPTTTISKFFELYPSVFIQFQPSLGLHPHVKITSQALLLHKEESTIHNSRPHRD 126

Query: 224 DVVKRLAKLLMLTGARKLPLYVVERLQWDLGLPYKFIPTLLADYPEYFQVCSMRDCSTGE 283
           DVVKRLAKLLMLTGA KLPLYV+E+LQWDLGLPY+FIPTLLADYP+YFQVCS++DC TGE
Sbjct: 127 DVVKRLAKLLMLTGAGKLPLYVIEKLQWDLGLPYRFIPTLLADYPDYFQVCSVKDCLTGE 186

Query: 284 QTLAFELLSWRKNLSVSELKKRECLEGNGWSKNGNHIAFPMSFPRGFDLEKKVLNWVEEW 343
           QTLA ELLSWRK+L+VSELKKRE LEGN  S+  N IAFPMSFPRGFDL+KKV+NWVEEW
Sbjct: 187 QTLALELLSWRKDLAVSELKKREYLEGNFGSRKRNRIAFPMSFPRGFDLQKKVMNWVEEW 246

Query: 344 QRLPYISPYENAFHLAPNSDQAEKWAVAVLHELLCLMVSKKTEKENIMCLGEYLGFGSRF 403
           Q LPYISPYENAFHLAPNSDQAEKWAVAVLHELL L +SKKTEKENI CLG+YLGFGSRF
Sbjct: 247 QHLPYISPYENAFHLAPNSDQAEKWAVAVLHELLYLTISKKTEKENIYCLGDYLGFGSRF 306

Query: 404 KKALVHHPGIFYVSNKIRTQTVVLREAYKKDFLVEKHPLMGMRHQYIHLMNKVVRKPTPG 463
           KKA+VHHPGIFYVSNKIRTQTVVLREAYKKDFL+EKHPLMGMRHQY+HLMNKV+RKP PG
Sbjct: 307 KKAIVHHPGIFYVSNKIRTQTVVLREAYKKDFLLEKHPLMGMRHQYLHLMNKVIRKPRPG 366

Query: 464 IILASSRGKRQNNSFANKQ 483
           IILASSRGKR+N + ANK+
Sbjct: 367 IILASSRGKRRNIALANKE 385

BLAST of Spg030221 vs. ExPASy TrEMBL
Match: A0A1S3C4Q9 (protein ROOT PRIMORDIUM DEFECTIVE 1 OS=Cucumis melo OX=3656 GN=LOC103496453 PE=4 SV=1)

HSP 1 Score: 674.5 bits (1739), Expect = 3.3e-190
Identity = 325/379 (85.75%), Postives = 356/379 (93.93%), Query Frame = 0

Query: 104 RLPYRLSQTRAFVDAKVKWVRDPYLDFAVQREKNLRQVISLKNLIILSPFKSLPLSSVSL 163
           R+P+R +Q RAFVDAKVKWVRDPYLDFAVQREKNL+QVISLKN+II SP  S+PLSSVSL
Sbjct: 7   RIPHRFTQIRAFVDAKVKWVRDPYLDFAVQREKNLKQVISLKNIIISSPLTSVPLSSVSL 66

Query: 164 LKQNLKVPTTTISKFFQLYPSVFIQFQPNLGLHPHVRLTPQALALHKEESTIHNSQPHRD 223
           LKQNLKVPTTTISKFF+LYPSVFIQFQP+LGLHPHV++T QAL LHKEESTIHNS+PHRD
Sbjct: 67  LKQNLKVPTTTISKFFELYPSVFIQFQPSLGLHPHVKITSQALLLHKEESTIHNSRPHRD 126

Query: 224 DVVKRLAKLLMLTGARKLPLYVVERLQWDLGLPYKFIPTLLADYPEYFQVCSMRDCSTGE 283
           DVVKRLAKLLMLTGA KLPLYV+E+LQWDLGLPY+FIPTLLADYP+YFQVCS++DC TGE
Sbjct: 127 DVVKRLAKLLMLTGAGKLPLYVIEKLQWDLGLPYRFIPTLLADYPDYFQVCSVKDCLTGE 186

Query: 284 QTLAFELLSWRKNLSVSELKKRECLEGNGWSKNGNHIAFPMSFPRGFDLEKKVLNWVEEW 343
           QTLA ELLSWRK+L+VSELKKRE LEGN  S+  N IAFPMSFPRGFDL+KKV+NWVEEW
Sbjct: 187 QTLALELLSWRKDLAVSELKKREYLEGNFGSRKRNRIAFPMSFPRGFDLQKKVMNWVEEW 246

Query: 344 QRLPYISPYENAFHLAPNSDQAEKWAVAVLHELLCLMVSKKTEKENIMCLGEYLGFGSRF 403
           Q LPYISPYENAFHLAPNSDQAEKWAVAVLHELL L +SKKTEKENI CLG+YLGFGSRF
Sbjct: 247 QHLPYISPYENAFHLAPNSDQAEKWAVAVLHELLYLTISKKTEKENIYCLGDYLGFGSRF 306

Query: 404 KKALVHHPGIFYVSNKIRTQTVVLREAYKKDFLVEKHPLMGMRHQYIHLMNKVVRKPTPG 463
           KKA+VHHPGIFYVSNKIRTQTVVLREAYKKDFL+EKHPLMGMRHQY+HLMNKV+RKP PG
Sbjct: 307 KKAIVHHPGIFYVSNKIRTQTVVLREAYKKDFLLEKHPLMGMRHQYLHLMNKVIRKPRPG 366

Query: 464 IILASSRGKRQNNSFANKQ 483
           IILASSRGKR+N + ANK+
Sbjct: 367 IILASSRGKRRNIALANKE 385

BLAST of Spg030221 vs. TAIR 10
Match: AT4G24320.1 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 453.8 bits (1166), Expect = 1.8e-127
Identity = 236/376 (62.77%), Postives = 283/376 (75.27%), Query Frame = 0

Query: 102 TVRLPYRLSQTRAFVDAKVKWVRDPYLDFAVQREKNLRQVISLKNLIILSPFKSLPLSSV 161
           T +L   L Q R FV+A+VKWV D YLD AVQREKNL+QVISLK+ I+ SP KSLPLSS+
Sbjct: 5   TTKLARALQQRRTFVNARVKWVSDHYLDEAVQREKNLKQVISLKDRIVSSPSKSLPLSSL 64

Query: 162 SLLKQ--NLKVPTTTISKFFQLYPSVFIQFQPNLGLHPHVRLTPQALALHKEESTIHNSQ 221
           SLLK   NL +   T + FFQ YPSVF  FQP+     HVRLTPQAL LHKEE TIH S 
Sbjct: 65  SLLKPLVNLHI---TAAAFFQKYPSVFTTFQPSPSHPLHVRLTPQALTLHKEEETIHLSP 124

Query: 222 PHRDDVVKRLAKLLMLTGARKLPLYVVERLQWDLGLPYKFIPTLLADYPEYFQVCSMRDC 281
           P R+  V+RL K LMLTGA  LPLYV++R ++DLGLP  +I +L+ DYPEYF+V  ++D 
Sbjct: 125 PQRNVTVQRLTKFLMLTGAGSLPLYVLDRFRFDLGLPRDYITSLIGDYPEYFEVTEIKDR 184

Query: 282 STGEQTLAFELLSWRKNLSVSELKKRECLEGNGWSKNGNHIAFPMSFPRGFDLEKKVLNW 341
            TGE+TLA  + S R NL VSE+++RE        K G  I + M+FP+G++L K+V NW
Sbjct: 185 LTGEKTLALTISSRRNNLPVSEMERREATIDGSRVKKGLRIRYSMNFPKGYELHKRVKNW 244

Query: 342 VEEWQRLPYISPYENAFHLAPNSDQAEKWAVAVLHELLCLMVSKKTEKENIMCLGEYLGF 401
           VE+WQ LPYISPYENAFHL   SDQAEKWAVAVLHELL L+VSKKTE +N++CLGEYLGF
Sbjct: 245 VEQWQNLPYISPYENAFHLGSYSDQAEKWAVAVLHELLFLLVSKKTETDNVICLGEYLGF 304

Query: 402 GSRFKKALVHHPGIFYVSNKIRTQTVVLREAYKKDFLVEKHPLMGMRHQYIHLMNKVVRK 461
           G RFKKALVHHPGIFY+S+KIRTQTVVLREAY K FL+EKHPLMG+RHQYI+LM+K    
Sbjct: 305 GMRFKKALVHHPGIFYMSHKIRTQTVVLREAYHKVFLLEKHPLMGIRHQYIYLMSK---- 364

Query: 462 PTPGIILASSRGKRQN 476
                   S RGK+++
Sbjct: 365 --------SGRGKKRD 365

BLAST of Spg030221 vs. TAIR 10
Match: AT1G71850.1 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 316.6 bits (810), Expect = 3.4e-86
Identity = 163/357 (45.66%), Postives = 240/357 (67.23%), Query Frame = 0

Query: 108 RLSQTRAFVDAKV-----KWVRDPYLDFAVQREKNLRQVISLKNLIILSPFKSLPLSSVS 167
           R S  R F D        K+VRD  LD AV+REKNLR ++S+K+LI   P KS+P+S ++
Sbjct: 12  RHSGHRTFADTSAVEDTYKFVRDRGLDHAVEREKNLRPLLSIKDLIRSEPAKSVPISVIT 71

Query: 168 LLKQNLKVPTTTISKFFQLYPSVFIQFQP-NLGLHPHVRLTPQALALHKEESTIHNSQPH 227
             K +L+VP   I +F + +PSVF +F P  +G+HPH+ LTP+ L    +E  ++ S+ +
Sbjct: 72  SQKDSLRVPLRPI-EFIRSFPSVFQEFLPGGIGIHPHISLTPEILNHDADEQLVYGSETY 131

Query: 228 RDDVVKRLAKLLMLTGARKLPLYVVERLQWDLGLPYKFIPTLLADYPEYFQV--CSMRDC 287
           +  +  RL KLLM+    K+PL +++ L+WDLGLP  ++ T++ ++P+YF+V    +R C
Sbjct: 132 KQGLADRLLKLLMINRINKIPLEILDLLKWDLGLPKDYVETMVPEFPDYFRVIKSKLRGC 191

Query: 288 STGEQTLAFELLSWRKNLSVSELKKRECLEGNGWSKNGNHIAFPMSFPRGFDLEKKVLNW 347
           S GE     EL+ W    +VS L+K+      G    G+ IAFPM F  GF ++KK+  W
Sbjct: 192 S-GE----LELVCWSNEHAVSVLEKKARTLRKGEYTKGSAIAFPMKFSNGFVVDKKMKKW 251

Query: 348 VEEWQRLPYISPYENAFHLAPNSDQAEKWAVAVLHELLCLMVSKKTEKENIMCLGEYLGF 407
           +++WQ+LPYISPYENA HL+  SD+++KWA AVLHE++ L VSKK EK+ I+ LGE++G 
Sbjct: 252 IDDWQKLPYISPYENALHLSATSDESDKWAAAVLHEIMNLFVSKKVEKDAILHLGEFMGL 311

Query: 408 GSRFKKALVHHPGIFYVSNKIRTQTVVLREAYKKDFLVEKHPLMGMRHQYIHLMNKV 457
            SRFK+ L +HPGIFY+S+K+RT TVVLR+ YK+  L+E + L+  R++Y+ LMN V
Sbjct: 312 RSRFKRVLHNHPGIFYLSSKLRTHTVVLRDGYKRGMLIESNELVTSRNRYMKLMNTV 362

BLAST of Spg030221 vs. TAIR 10
Match: AT5G48040.1 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 247.7 bits (631), Expect = 1.9e-65
Identity = 142/378 (37.57%), Postives = 220/378 (58.20%), Query Frame = 0

Query: 104 RLPYRLSQTRAFVDAKVKWVRDPYLDFAVQREKNLRQVISLKNLIILSPFKSLPLSSVSL 163
           R  +   Q    V+ K+KWV+D  LD  V REK+LR V +L ++I  SP   LP+  +  
Sbjct: 21  RFDFFYQQRCGLVNVKLKWVKDRELDAVVVREKHLRAVCNLVSVISASPDLRLPIFKLLP 80

Query: 164 LKQNLKVP-TTTISKFFQLYPSVFIQ--FQPNLGLH-PHVRLTPQALALHKEESTIHNSQ 223
            +  L +P    +S F + YP++F++  +  + G   P   LT + + L+ EE  +  S+
Sbjct: 81  HRGQLGLPQELKLSAFIRRYPNIFVEHCYWDSAGTSVPCFGLTRETIDLYYEEVDV--SR 140

Query: 224 PHRDDVVKRLAKLLMLTGARKLPLYVVERLQWDLGLPYKFIPTLLADYPEYFQVCSMRDC 283
            +  DV+ RL KLLMLT  R L L+ ++ L+WDLGLPY +  +L+  +P+ F +  +   
Sbjct: 141 VNERDVLVRLCKLLMLTCERTLSLHSIDHLRWDLGLPYDYRDSLITKHPDLFSLVKLSSD 200

Query: 284 STGEQTLAFELLSWRKNLSVSELKKRECLEGNGWSKNGNHIAFPMSFPRGFDLEKKVLNW 343
             G      +L+ W ++L+VS+++ RE +       N   +AFP+ F RGF L++K + W
Sbjct: 201 LDG-----LKLIHWDEHLAVSQMQLREDV------GNDERMAFPVKFTRGFGLKRKSIEW 260

Query: 344 VEEWQRLPYISPYENAFHLAPNSDQAEKWAVAVLHELLCLMVSKKTEKENIMCLGEYLGF 403
           ++EWQRLPY SPY +A HL P +D +EK  V V HELL L + KKTE++N+  L +    
Sbjct: 261 LQEWQRLPYTSPYVDASHLDPRTDLSEKRNVGVFHELLHLTIGKKTERKNVSNLRKPFAL 320

Query: 404 GSRFKKALVHHPGIFYVSNKIRTQTVVLREAYKKDFLVEKHPLMGMRHQYIHLMNKVVRK 463
             +F K    HPGIFY+S K  TQTV+LREAY +  L+EKHPL+ +R ++ ++MN+    
Sbjct: 321 PQKFTKVFERHPGIFYISMKCDTQTVILREAYDRRHLIEKHPLVEVREKFANMMNEGFLD 380

Query: 464 PTPGIILASSRGKRQNNS 478
            + G+   S     + N+
Sbjct: 381 RSRGLYQKSVEADLEKNN 385

BLAST of Spg030221 vs. TAIR 10
Match: AT2G39120.1 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 216.5 bits (550), Expect = 4.8e-56
Identity = 131/348 (37.64%), Postives = 199/348 (57.18%), Query Frame = 0

Query: 109 LSQTRAFVDAKVKWVRDPYLDFA--VQREKNLRQVISLKNLIILSPFKSLPLSSVSLLKQ 168
           L+Q R +VD  +KW RDPY D    + R   L+ V+SLKN I+  P + +P+S++S   +
Sbjct: 18  LTQKRTYVDVYMKWKRDPYFDNIEHILRSSQLKSVVSLKNCIVQEPNRCIPISAISKKTR 77

Query: 169 NLKVPTTTISKFFQLYPSVFIQF-QPNLGLHPHVRLTPQALALHKEESTIHNSQPHRDDV 228
              V +T I+ F + +PS+F +F  P   L P  RLTP+A  L ++E  ++  Q   DD+
Sbjct: 78  QFDV-STKIAHFLRKFPSIFEEFVGPEYNL-PWFRLTPEATELDRQERVVY--QTSADDL 137

Query: 229 VKRLAKLLMLTGARKLPLYVVERLQWDLGLPYKFIPTLLADYPEYFQVCSMRDCSTGEQT 288
             RL KL++++    LPL +V+ ++W LGLP  ++     +    F+   M D   G + 
Sbjct: 138 RDRLKKLILMSKDNVLPLSIVQGMKWYLGLPDDYLQFPDMNLDSSFRFVDMED---GVKG 197

Query: 289 LAFELLSWRKNLSVSELKKRECLEGNGWSKNGNHIAFPMSFPRGFDLEKKVLNWVEEWQR 348
           LA +     K LSV  L+K    +  G   +   I FP+   +G  L  K+ +W+ E+Q+
Sbjct: 198 LAVDYNGGDKVLSV--LQKNAMKKRRG-EVSLEEIEFPLFPSKGCRLRVKIEDWLMEFQK 257

Query: 349 LPYISPYENAFHLAPNSDQAEKWAVAVLHELLCLMVSKKTEKENIMCLGEYLGFGSRFKK 408
           LPY+SPY++   L P+SD AEK  V  LHELLCL V    E++ ++CL ++ G   +  K
Sbjct: 258 LPYVSPYDDYSCLDPSSDIAEKRVVGFLHELLCLFVEHSAERKKLLCLKKHFGLPQKVHK 317

Query: 409 ALVHHPGIFYVSNKIRTQTVVLREAYKKDFLVEKHPLMGMRHQYIHLM 454
           A   HP IFY+S K +T T +LRE Y+    VE HP++G+R +YI LM
Sbjct: 318 AFERHPQIFYLSMKNKTCTAILREPYRDKASVETHPVLGVRKKYIQLM 355

BLAST of Spg030221 vs. TAIR 10
Match: AT3G58520.1 (Ubiquitin carboxyl-terminal hydrolase family protein )

HSP 1 Score: 212.2 bits (539), Expect = 9.1e-55
Identity = 126/349 (36.10%), Postives = 199/349 (57.02%), Query Frame = 0

Query: 114 AFVDAKVKWVRDPYLDFAVQREKNLRQVISLKNLIILSPFKSLPLSSVSLLKQNLKVPTT 173
           A    ++KWV++  LD  +  E +L+    LK+ I  SP   L   SV+  ++ L + T 
Sbjct: 27  AMTKVRLKWVKNKNLDHVIDTETDLKAACILKDAIKRSPTGFLTAKSVADWQKLLGL-TV 86

Query: 174 TISKFFQLYPSVFIQF-QPNLGLHPHVRLTPQALALHKEESTIHNSQPHRDDVVKRLAKL 233
            + +F + YP++F +F        P  +LT  AL L  +E  IH  Q H  D V+RL ++
Sbjct: 87  PVLRFLRRYPTLFHEFPHARYASLPCFKLTDTALMLDSQEEIIH--QSHEADTVERLCRV 146

Query: 234 LMLTGARKLPLYVVERLQWDLGLPYKFIPTLLADYPEYFQVCSMRDCSTGEQTLAFELLS 293
           LM+  ++ + L  +  L++DLGLP  +  TL+  YP++F  C ++  S G   L  +L+ 
Sbjct: 147 LMMMRSKTVSLRSLHSLKFDLGLPDNYEKTLVMKYPDHF--CFVK-ASNGNPCL--KLVK 206

Query: 294 WRKNLSVSELKKR--------ECLEGNGWSKNGNHIAFPMSFPRGFDLEKKVLNWVEEWQ 353
           WR   + S L+KR        E      + +  + + FPMSFPRG+  +KKV  W++E+Q
Sbjct: 207 WRDEFAFSALQKRNEDNDVSGEESRYREFKRGQSTLTFPMSFPRGYGAQKKVKAWMDEFQ 266

Query: 354 RLPYISPYENAFHLAPNSDQAEKWAVAVLHELLCLMVSKKTEKENIMCLGEYLGFGSRFK 413
           +LPYISPY++  ++ P SD  EK AVAVLHELL L + KKT++  +  +   L    +F 
Sbjct: 267 KLPYISPYDDPSNIDPESDLMEKRAVAVLHELLSLTIHKKTKRNYLRSMRAELNIPHKFT 326

Query: 414 KALVHHPGIFYVSNKIRTQTVVLREAYKKDFLVEKHPLMGMRHQYIHLM 454
           +    +PGIFY+S K +T TV+L+E Y++  LV+ HPL  +R ++ H+M
Sbjct: 327 RLFTRYPGIFYLSLKCKTTTVILKEGYRRGKLVDPHPLTRLRDKFYHVM 367